Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Coline Sivelle
Date created: 2016-10-12 11:00:00
Date modified: 2016-10-14 09:02:50
Dimerization system FRB/FKBP linked to two subunit of tripartite GFP (GFP 10 & GFP 11)
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K2039002_sequence (Version 1) |
Description
This part is the association of part K2039000 and K2039001.The FRB/FKBP12 system is an inducible system. Originally found in mammals, these two proteins form an heterodimer when rapamycin is added in the middle, it is particularly used in protein interaction studies. The FRB sequence has been modified in order to dimerize with a non toxic rapamycin analog (rapalog). The mutations introduced are : T2098L, K2095P, W2101F. It has also been codon optimized with Jcat plateforme to be expressed in bacteria. FRB* protein is linked to GFP 11. The tripartite split-GFP is composed of two times of 20 amino-acids long GFP tags (GFP 10 and GFP 11) and a third complementary subsection (GFP 1-9).
Notes
Codon optomization for E. coliSource
Composite part| Access | Instance | Definition |
| public public | BBa_K2039001 BBa_K2039000 | BBa_K2039001 BBa_K2039000 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K2039001 BBa_K2039000 | 1,701 710,1440 | BBa_K2039001 BBa_K2039000 |