Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Maxence Pfeiffer
Date created: 2016-10-11 11:00:00
Date modified: 2016-10-14 12:58:32
Part expressing the dimerization protein FKBP and GFP10 (subunit of tripartite GFP)
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K2039000_sequence (Version 1) |
Description
This part is the complementary part of Bba_K2039001.The FRB/FKBP12 system is an inducible system. Originally found in mammals, these two proteins form an heterodimer when rapamycin is added in the middle, it is particularly used in protein interaction studies. The FRB sequence has been optimized in order to be expressed in bacteria: mutations (T2098L, K2095P, W2101F) for dimerization with rapalog and optimization by Jcat plateforme.
FKBP protein is linked to GFP 10.
The tripartite split-GFP is composed of two times of 20 amino-acids long GFP tags (GFP 10 and GFP 11) and a third complementary subsection (GFP 1-9).
Notes
No issuesSource
Synthetic| Access | Instance | Definition |
| public public public public | BBa_J23119 BBa_B0030 BBa_K2039003 BBa_B0015 | BBa_J23119 BBa_B0030 BBa_K2039003 BBa_B0015 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23119 BBa_B0030 BBa_K2039003 BBa_B0015 | 1,35 44,58 67,594 603,731 | BBa_J23119 BBa_B0030 BBa_K2039003 BBa_B0015 |