BBa_K1361005

BBa_K1361005 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1361005
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Shoujie Sun
Date created: 2014-10-05 11:00:00
Date modified: 2015-05-08 01:10:04

CsgE, CsgF, CsgG, the outer membrane secrete device for curli fiber, at relatively low constitutive



    • Details

    Types
    DnaRegion

    Roles
    Generator

    engineered_region

    Sequences BBa_K1361005_sequence (Version 1)

    Description

    This part is for specific transport of CsgA(BBa_K1361999) and CsgB(BBa_K1361997) into extracellular matrix. CsgEFG(BBa_K1361992) was placed after a relatively weak constitutive promoter.

    Notes

    The CsgEFG was PCRed as a whole. The EcoRI loci within CsgA sequence has been replaced from GAATTC to GAATTT.

    Source

    PCR on genomic DNA from E coli. DH5alpha strain. And site-directed mutation by overlap PCR primers.

    Access Instance Definition
    public
    public
    		BBa_K608006
    BBa_K1361992
    		BBa_K608006
    BBa_K1361992

    Sequence Annotation Location Component / Role(s)
    BBa_K608006
    BBa_K1361992
    		1,56
    63,1753
    		BBa_K608006
    BBa_K1361992

    igem#sampleStatus
    It's complicated
    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K1361005/1
     

    Attachments

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