Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Shoujie Sun
Date created: 2014-10-05 11:00:00
Date modified: 2015-05-08 01:10:04
CsgE, CsgF, CsgG, the outer membrane secrete device for curli fiber
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1361992_sequence (Version 1) |
Description
This part is for specific transport of CsgA(BBa_K1361999) and CsgB(BBa_K1361997) into extracellular matrix.CsgE is a small periplasmic protein, is proposed to direct CsgA to the CsgG secretion complex and helps mediate secretion specificity via an N-terminal sequence in the mature CsgA.
CsgF is a membrane-associated protein, required for CsgB surface exposure, and is crucial for efficient CsgA polymerization.
CsgG is a canal protein at outer membrane that is required for secretion of CsgA and CsgB into the extracellular milieu.
Notes
The CsgEFG was PCRed as a whole, despite there may be some regulation sequence between them. The EcoRI loci within CsgA sequence has been replaced from GAATTC to GAATTT.Source
PCR on genomic DNA from E coli. DH5alpha strain. And site-directed mutation by overlap PCR primers.| Sequence Annotation | Location | Component / Role(s) |
| CsgE CsgF CsgG start stop start stop start stop | 1,390 415,831 858,1691 1,3 388,390 415,417 829,831 858,860 1689,1691 | CDS feature/cds feature/cds CDS feature/cds CDS start_codon feature/start stop_codon feature/stop start_codon feature/start feature/stop stop_codon start_codon feature/start stop_codon feature/stop |