Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Tom Knight
Date created: 2004-05-25 11:00:00
Date modified: 2015-05-08 01:14:55
pSB1A2 (Replaced by pSB1A3)
| Types | DnaRegion |
| Roles | Plasmid_Backbone plasmid_vector |
| Sequences | pSB1A2_sequence (Version 1) |
Description
pSB1A2 is a high copy number plasmid carrying ampicillin resistance.The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell).
pSB1A2 has one terminator upstream of its MCS, which is oriented to prevent transcription from *inside* the MCS from reading out into any DNA outside the MCS. The orientation of the pBLA promoter driving the AmpR gene is such that relatively little transcription should be coming into the MCS from upstream. Ideally, a future version of the standard biobrick vectors would have terminators bracketing an MCS that were 100% efficient in terminating both into and out of the MCS region.
| Sequence Annotation | Location | Component / Role(s) |
| Prefix Suffix VR ORI rep (pMB1) (rev) APr (rev) pBLA (rev) VF2 terminator | 2058,2079 1,21 79,98 198,198 183,797 957,1817 1852,1852 1940,1959 2029,2048 | feature/BioBrick engineered_region engineered_region feature/BioBrick feature/primer_binding primer_binding_site sequence_feature feature/misc feature/cds CDS feature/cds CDS sequence_feature feature/misc feature/primer_binding primer_binding_site stem_loop feature/stem_loop |