Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Daniel Bui and Aaditya Shidham
Date created: 2010-05-31 11:00:00
Date modified: 2015-05-08 01:14:01
CDS for Fnr-LH28 protein, activates narGp in presence of nitrate.
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_M33002_sequence (Version 1) |
Description
The fnr gene naturally is activated under anaerobic conditions during E. coli nitrogen respiration. The part above contains a single missense LH28 mutation to allow it to operate under aerobic conditions. Cytoplasmic Fnr-LH28 protein created by the gene can bind to a regulatory region upstream of the nar operon as an activator, facilitating the reduction of nitrate to nitrite.Notes
The coding sequence was checked for restriction enzyme sites to ensure that it is Assembly Standard 10-compliant.Source
The part comes from the E. coli genome.| Sequence Annotation | Location | Component / Role(s) |
| fnr-LH28 Start Codon T to A LH28 Mutation Stop Codon | 1,756 1,3 83,83 751,756 | feature/cds CDS feature/start start_codon feature/mutation sequence_alteration stop_codon feature/stop |