Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: KUO, CHIA-CHUN
Date created: 2010-10-21 11:00:00
Date modified: 2015-05-08 01:12:05
Constitutive promoter + 37℃
induced RBS + tetR + double terminator
| Types | DnaRegion |
| Roles | engineered_region Generator |
| Sequences | BBa_K332031_sequence (Version 1) |
Description
we took advantage of the interaction between 37?? C induced RBS, tetR gene and pTet promoter to create the temperature regulating circuit.The following is a description of our prescribed strategy.
First, we use the constitutive promoter, 37?? C induced RBS (K115002), the tetR gene and terminators.
We will call this part the ???Regulator???.
We speculate that when the ???Regulator??? and ???Production??? sections are combined, we can achieve a high bacteria growth rate and close to no target protein producing rate at T>37??C, and low bacteria growth rate and high target protein producing rate at T<37??C.
Notes
To regulate following gene by temperatureSource
iGEM08_TUDelft| Access | Instance | Definition |
| public public public public | BBa_J23101 BBa_K115002 BBa_C0040 BBa_J61048 | BBa_J23101 BBa_K115002 tetR BBa_J61048 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23101 BBa_K115002 BBa_C0040 BBa_J61048 | 1,35 44,95 102,786 795,907 | BBa_J23101 BBa_K115002 tetR BBa_J61048 |