BBa_K243025

BBa_K243025 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K243025

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Freiburg Bioware09

Date created: 2009-10-17 11:00:00

Date modified: 2015-05-08 01:11:37

Strep-FluA-Middle Linker-Fok_i

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_K243025_sequence (Version 1)

Description

This combination of parts use the benefits of an Strep-tag and is linked with a FluroescineA tag. The MiddleLinker (GlySerGlyGly)x2 produce a certain distance between the FluA tag and the protein domain Fok_i.

Notes

The linker length and the choice of the purification tag in combination with the anticalin tag allowed us several combination possibilities, which we had to prove to achieve the best combination.

Source

Combined by serial clonig steps.

Components

Access	Instance	Definition
public	BBa_K157012	BBa_K157012
public	BBa_B0105	Scar 25
public	BBa_K157004	BBa_K157004
public	BBa_B0105	Scar 25
public	BBa_K243005	MiddleLink
public	BBa_B0105	Scar 25
public	BBa_K243001	Fok_i

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K157012	1,24	BBa_K157012
BBa_B0105	25,30	Scar 25
BBa_K157004	31,552	BBa_K157004
BBa_B0105	553,558	Scar 25
BBa_K243005	559,582	MiddleLink
BBa_B0105	583,588	Scar 25
BBa_K243001	589,1167	Fok_i

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K243025/1

Member of these Collections

• iGEM Parts Registry