Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: John Heil
Date created: 2009-10-19 11:00:00
Date modified: 2015-05-08 01:11:37
Nuclease construct, no sense promoter.
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K241000_sequence (Version 1) |
Description
To tightly repress the nuclease genes (T7 gene 6, PmeI) an antisense promoter and the cI repressor are used.The promoter for expression of the integrase plasmid (the sense promoter) is not on this part. However, the design calls for the Lambda Pr promoter.
The part is designed to selectively degrade DNA in vivo.
Notes
The main design consideration was to have maximal repression of the toxic nuclease genes.Source
Synthetic, Phage T7, Phage Lambda, Pseudomonas mendocina.| Access | Instance | Definition |
| public public public public public public public | BBa_B0034 BBa_K093001 BBa_K093013 BBa_K093004 BBa_R0011 BBa_K093006 BBa_B0015 | BBa_B0034 T7Gene6 RBS-PmeIR rPlac+TT lacI+pL RBS-cI BBa_B0015 |