BBa_K2100053

BBa_K2100053 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K2100053

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Wangui Mbuguiro

Date created: 2016-10-18 11:00:00

Date modified: 2016-10-24 04:38:01

Sensor 142-5p

Details

• Types: DnaRegion
• Roles: sequence_feature; DNA
• Sequences: BBa_K2100053_sequence (Version 1)

Description

This part contains two main transcriptional units. One encoding hef1a-mKate adjacent to 4 miRNA target sites. The other encodes hef1a-BFP. mKate production signifies the amount of repression occurring due to miRNA activity. BFP can be used as transfection marker.

Notes

Courtesy of Jeremy Gam of MIT's Weiss Lab

Source

human genome

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
miR-142-5p target site	2,93	sequence_feature, feature/misc
hEF1A	1461,2634	promoter, feature/promoter
eBFP	2690,3409	feature/protein, CDS
hEF1A	9030,10203	feature/promoter, promoter
mKate	10268,10972	feature/promoter, promoter

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2100053/1

Member of these Collections

• iGEM Parts Registry