Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
Date created: 2016-08-28 11:00:00
Date modified: 2016-10-12 01:00:47
pTet GFP + UNS
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K2066023_sequence (Version 1) |
Description
This part is essentially R0040 driving expression of GFP. It is K1493504 on a UNS backbone. TetR is a repressor molecule that can bind to the pTET promoter and block the transcription of GFP. Used this part with BBa_K2066022 to look at aTc induction.Notes
Designed this part to put K1493504 on the UNS backbone.Source
Sequence was synthesized using parts from the iGEM registry as well as UNS spacer sequences from Torella et. al (2013)| Access | Instance | Definition |
| public public public public public public public public | BBa_K2066018 BBa_R0040 BBa_K2066529 BBa_K2066527 BBa_E0040 BBa_K2066529 BBa_B0015 BBa_K2066019 | BBa_K2066018 p(tetR) BBa_K2066529 BBa_K2066527 GFP BBa_K2066529 BBa_B0015 BBa_K2066019 |