nprb

BBa_K2029000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K2029000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Max Schelski, Niklas Schmacke

Date created: 2016-08-28 11:00:00

Date modified: 2016-08-29 12:12:46

P43_RBS_nprb

Details

• Types: DnaRegion
• Roles: Composite; engineered_region
• Sequences: BBa_K2029000_sequence (Version 1)

Description

Part consisting of the P43 promotor, RBS and coding sequence of the nprb signal peptide which induces secretion in B. subtilis. This part can be used to produce and secrete proteins in B. subtilis.

Notes

standard iGEM cloning site was replaced with analoge for scarless cloning

Source

p43_RBS_nprb sequence was taken from the p43NMK plasmid: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1169017/

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
p43 promotor	1,280	feature/promoter, promoter
RBS	281,290	feature/rbs, ribosome_entry_site
start codon	300,302	start_codon, feature/start
nprb signal peptide	303,380	feature/misc, sequence_feature

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2029000/1

Member of these Collections

• //promoter
• //rbs/prokaryote/constitutive
• iGEM Parts Registry