Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Marco V. Lincango Y.
Date created: 2016-10-17 11:00:00
Date modified: 2016-10-18 01:09:27
spisPink poly his tag
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K2004013_sequence (Version 1) |
Description
In this way, a brief step to confirm the chromoprotein expression is purifying it, so to facilitate this process we fuse a improved version of the sequence (BBa_K2004004) that codifies to pink chromoprotein with another registered part, the poly his tag (BBa_K844000). The last version of this chromoprotein(BBa_K1684000), designed by the previous Biosint team, was analyzed according to its sequence and all the parameter that must meet to be part of a standardized biobrick. This device (BBa_K2004013) allows to test the fusion, because contains a constitutive promoter and a RBS upstream and the double terminator downstream.Notes
It is a improved and correct version of the protein spispink, to be able to use like a biobrick.Source
Expressed in Stylophora pistillata, was evolving independently from the rest of coral chromoproteins.| Access | Instance | Definition |
| public public public public public | BBa_J23101 BBa_J61101 BBa_K2004004 BBa_K844000 BBa_B0015 | BBa_J23101 BBa_J61101 BBa_K2004004 BBa_K844000 BBa_B0015 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23101 BBa_J61101 BBa_K2004004 BBa_K844000 BBa_B0015 | 1,35 44,55 62,733 742,777 786,914 | BBa_J23101 BBa_J61101 BBa_K2004004 BBa_K844000 BBa_B0015 |