Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Yusuke Banno
Date created: 2015-09-09 11:00:00
Date modified: 2015-09-17 08:36:00
mRNA circularize efficiently generator (inside complementarityⅠ
)
| Types | DnaRegion |
| Roles | engineered_region Generator |
| Sequences | BBa_K1859024_sequence (Version 1) |
Description
This device is all-in-one mRNA circularization device. If you transfer this device into E. coli, you can get a protein (its sequence is ???-HHHHHH - amino acid derived from RBS sequence - HisRFP ??? GGSGGS - amino acid derived from ribozyme sequence ??? HHHHHH - amino acid derived from RBS sequence -???). Two types of the sequence derived from td gene are complementarity sequences with each other. So we are hoping for an improvement of the efficiency of circularization.HHHHHH－GGSGGS
Notes
This part includes complementary sequences.Source
T4 phage| Access | Instance | Definition |
| public public public public public public | BBa_R0010 BBa_K1859003 BBa_B0034 BBa_K1332002 BBa_K1859004 BBa_B0015 | LacI BBa_K1859003 BBa_B0034 BBa_K1332002 BBa_K1859004 BBa_B0015 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_R0010 BBa_K1859003 BBa_B0034 BBa_K1332002 BBa_K1859004 BBa_B0015 | 1,200 209,411 420,431 440,1142 1151,1280 1289,1417 | LacI BBa_K1859003 BBa_B0034 BBa_K1332002 BBa_K1859004 BBa_B0015 |