Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: M??nica Victoria Guti??rrez Salazar
Date created: 2015-09-06 11:00:00
Date modified: 2015-12-16 01:55:00
35S:Gal4BD-DronpaK:T35S - 35S:DronpaN-VP16:T35S
| Types | DnaRegion |
| Roles | Device engineered_region |
| Sequences | BBa_K1742012_sequence (Version 1) |
Description
Translational unit composed by the promoter 35S, the T35s terminator, the CDS Dronpa(145K), Dronpa(145N), the binding domain Gal4 and VP16. The CDS works as a toggle switch activated with green light and deactivated with blue light. Dronpa(145K) changes its conformation with light, being able to dimerize with Dronpa(145N), acting as a transcription factor.Notes
Do a PCR to obtain the Dronpa(145N)Source
Synthesis| Access | Instance | Definition |
| public public public public public | BBa_K1467101 BBa_K105007 BBa_K1742002 BBa_K1742003 BBa_K105001 | BBa_K1467101 BBa_K105007 BBa_K1742002 BBa_K1742003 BBa_K105001 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1467101 BBa_K105007 BBa_K1742002 BBa_K1742003 BBa_K105001 | 1,1432 1441,1878 1885,2577 2586,3326 3335,3610 | BBa_K1467101 BBa_K105007 BBa_K1742002 BBa_K1742003 BBa_K105001 |