BBa_K165061

BBa_K165061 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K165061

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Aaron Glieberman

Date created: 2008-10-29 12:00:00

Date modified: 2015-05-08 01:10:56

pRS304* yeast shuttle vector, TRP1 selection

Details

• Types: DnaRegion
• Roles: plasmid; Plasmid
• Sequences: BBa_K165061_sequence (Version 1)

Description

This is a vector specifically designed for integration into a yeast genome at the LEU2 gene.


The final construction step of a Biobrick device can be made into this vector but cutting with the following scheme:


The system works in a yeast strain that has a mutation in the LEU2 gene, meaning that it is incapable of producing leucine on its own. Untransformed yeast cells with this mutation must be grown in media that contains leucine in order to be viable. However, after a transformation protocol in which competent yeast cells are treated with the pRS304* plasmid

Yeast integration

Notes

No design considerations as of yet.

Source

This is a modification of the Sikorski vector was described in R. S. Sikorski and P. Hieter, Genetics, 122: 19-27 (1989).

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K165061/1

Member of these Collections

• iGEM Parts Registry