Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jens Sivk??r Pettersen
Date created: 2015-09-13 11:00:00
Date modified: 2015-09-14 05:44:44
Yhbj fused to T25 domain of CyaA with cAMP-induced RFP reporter
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1638038_sequence (Version 1) |
Description
The RNase adapter protein YhbJ fused to the T25-domain of the adenylate cyclase, CyaA, from Bordetella pertussis. This construct can be used in the two-hybrid screening of protein-protein interactions between YhbJ and another x protein fused to the T18 domain. If a random set of peptide aptamers is conjugated to the T18-domain, one can screen for specific peptide aptamers against YhbJ.Notes
.Source
.| Access | Instance | Definition |
| public public | BBa_K861173 BBa_K1638026 | BBa_K861173 BBa_K1638026 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K861173 BBa_K1638026 | 1,1000 1009,2732 | BBa_K861173 BBa_K1638026 |