Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kao Jung Chang
Date created: 2014-10-07 11:00:00
Date modified: 2016-02-12 09:00:45
Dispersin generator circuit
| Types | DnaRegion |
| Roles | Conjugation oriT |
| Sequences | BBa_K1510214_sequence (Version 1) |
Description
he Dispersin gene codes for an enzyme which catalyzes the hydrolysis of the extracellular matrix produced by Gram negative bacteria.With strong constitutive promoter,together with ribosomal binding site and signal sequence yebF, make dispersin work more accurately.Notes
To fit igem part standard, the circuit is constructed in backbone pSB1C3, chloramphenicol with EcoR1 and Xba1 in front of the part, and Spe1, Pst1 behind the part.Source
The promoter, J23100, comes from the 2014 distributed igem kit. The signal sequence, yebF, encoding signal protein exporting recombinant protein extracellular, is from E.coli K-12. By designing primer, we obtain it together with its naturally occurring ribosomal binding site using PCR and extraction.The dispersin is from Lyon INSA igem team. Finally, the terminator B0015 is from 2014 igem distribution.| Access | Instance | Definition |
| public public public public public | BBa_J23100 BBa_K1510009 BBa_B0015 BBa_K802001 BBa_B0015 | BBa_J23100 BBa_K1510009 BBa_B0015 BBa_K802001 BBa_B0015 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23100 BBa_K1510009 BBa_B0015 BBa_K802001 BBa_B0015 | 1,35 44,584 1804,1916 593,1916 1925,2053 | BBa_J23100 BBa_K1510009 BBa_B0015 BBa_K802001 BBa_B0015 |