BBa_K1510002

BBa_K1510002 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1510002

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Yen-An Chang

Date created: 2014-10-05 11:00:00

Date modified: 2015-05-08 01:10:47

PnlmC (S.mutans)

Details

• Types: DnaRegion
• Roles: Regulatory; promoter
• Sequences: BBa_K1510002_sequence (Version 1)

Description

This part is derived from Streptococcus mutans UA159, a gram positive bacterium. The main function of this promoter is to sense a quorum sensing chemical, competence-stimulating peptide (CSP), and trigger down string gene.

Notes

Since there is no accurate description of actual position of nlmC promoter in previous study, to ensure the function of this promoter, we amplified the sequence from two comE binding site( used for detecting CSP) to the last nucleotide before gene nlmC. As a result, the gene sequence consists of two comE binding site, nlmC promoter and RBS.

Source

Streptococcus mutans UA159

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
comE binding site	11,21	feature/binding, non_covalent_binding_site
comE binding site	32,42	feature/binding, non_covalent_binding_site
-35 extend region	48,53	feature/binding, non_covalent_binding_site
-10 extend region	69,76	feature/binding, non_covalent_binding_site

Other Properties

• igem#sampleStatus: In stock
• igem#status: Available
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1510002/1

Member of these Collections

• iGEM 2018 Distribution Plate 5
• iGEM 2018 Distribution Plate 5 Well 6P
• iGEM 2018 Part Library
• iGEM 2018 Plasmid pSB1C3
• iGEM Parts Registry