Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kevin Yang
Date created: 2014-10-16 11:00:00
Date modified: 2015-05-08 01:10:46
ParoF--mCherry--dam
| Types | DnaRegion |
| Roles | engineered_region Translational_Unit |
| Sequences | BBa_K1500013_sequence (Version 1) |
Description
Dam is involved in methylating DNA, overexpression causes 6.4 * 10^-4 /// 6.4 * 10^4 mutation rate increase, mostly catalyzing transversions. It requires no mutations and simple overexpression is sufficient to cause its mutagenic activity. In this plasmid, it is placed downstream of the tyrosine-repressible promoter ParoF and reporter gene mCherry to fulfill our goals to improving an established system of directed evolution.Notes
NASource
Dam was cut from K1500994 and inserted into ParoF--mCherry backbone registry plasmid K1500001.| Access | Instance | Definition |
| public public public | BBa_K1500001 BBa_B0032 BBa_K1500994 | BBa_K1500001 BBa_B0032 BBa_K1500994 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1500001 BBa_B0032 BBa_K1500994 | 1,950 959,971 978,1814 | BBa_K1500001 BBa_B0032 BBa_K1500994 |