BBa_K1500008

BBa_K1500008 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1500008

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Kevin Yang

Date created: 2014-10-16 11:00:00

Date modified: 2015-05-08 01:10:46

IPTG-GFP mutated MutY

Details

• Types: DnaRegion
• Roles: engineered_region; Translational_Unit
• Sequences: BBa_K1500008_sequence (Version 1)

Description

The MutY gene cloned into the IPTG-GFP plasmid for characterization. It is involved in G-A mismatch DNA repair. Favors GC to TA Transversions when impaired. This is the dominant negative mutagenic version of the gene that contains a mutation from valine to alanine at the 45th residue.

Notes

NA

Source

It was cloned from wild-type DH5-alpha E. coli sequence and site-directed mutagenesis was performed in the IPTG-GFP plasmid.

Components

Access	Instance	Definition
public	BBa_K1500002	BBa_K1500002
public	BBa_B0032	BBa_B0032
public	BBa_K1500992	BBa_K1500992

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K1500002	1,946	BBa_K1500002
BBa_B0032	955,967	BBa_B0032
BBa_K1500992	974,2026	BBa_K1500992

Other Properties

• igem#sampleStatus: In stock
• igem#status: Available
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1500008/1

Member of these Collections

• iGEM Parts Registry