Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kevin Yang
Date created: 2014-10-16 11:00:00
Date modified: 2015-05-08 01:10:46
IPTG-GFP MutL
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1500006_sequence (Version 1) |
Description
The nonmutated, wild-type MutL gene cloned into the IPTG-GFP plasmid for characterization of mutation rate, verification of fucntionality, etc. MutL, long with MutS and MutH, initiates repair cascade by recognizing base mismatches/indels of up to 4 bp (dam-directed mismatch repair). It requires the mutation E32K to gain its active dominant negative mutagenic form.Notes
NASource
MutL from BBa_K1500997 was cut out and inserted downstream into the IPTG-GFP backbone plasmid (K1500006).| Access | Instance | Definition |
| public public public | BBa_K1500002 BBa_B0032 BBa_K1500997 | BBa_K1500002 BBa_B0032 BBa_K1500997 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1500002 BBa_B0032 BBa_K1500997 | 1,946 955,967 974,2821 | BBa_K1500002 BBa_B0032 BBa_K1500997 |