BBa_K131001

BBa_K131001 Version 1

Component

This part has been discontinued.

Source: http://parts.igem.org/Part:BBa_K131001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Kevin McLeod

Date created: 2008-07-21 11:00:00

Date modified: 2015-05-08 01:09:47

ColE2 SOS Promoter

Details

• Types: DnaRegion
• Roles: Regulatory; promoter
• Sequences: BBa_K131001_sequence (Version 1)

Description

This promoter normally represses the ColE2 activity gene, ceaB, in the ColE2 operon.

To activate this promoter would require a RecA positive strain, as the protein LexA represses the promoter. To induce this promoter, an intercalating agent, mitomycin C, was utilized to induce the SOS response, and thus the expression of the genes under control of this promoter.

Notes

Lab strains of E. coli, such as Top10 from Invitrogen, are deficient in RecA so we required a RecA positive strain to induce this promoter. We used BL21 from Novagen.

Source

This part comes from the ColE2 operon, which came from a plasmid.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
-35 Signal	218,224	feature/misc, sequence_feature
-15 Signal	241,246	sequence_feature, feature/misc
Region of Dyad Symmetry	136,161	sequence_feature, feature/misc
SOS Box	252,267	feature/misc, sequence_feature

Other Properties

• igem#experience: None
• igem#sampleStatus: Discontinued
• igem#status: Deleted
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K131001/1

Member of these Collections

• //chassis/prokaryote/ecoli
• //direction/forward
• //promoter
• //regulation/negative
• //rnap/prokaryote/ecoli/sigma70
• iGEM Parts Registry