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B-H-R plas

BBa_K125020 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K125020

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Margaret Ruzicka

Date created: 2008-10-27 12:00:00

Date modified: 2015-05-08 01:09:44

lac promoter/rbs/rep/oriV/lac promoter/rbs/aadA/oriT

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Details

Types	DnaRegion
Roles	sequence_feature Temporary
Sequences	BBa_K125020_sequence (Version 1)

Description

This is an intermediate construction step for the construction of [[Parts:BBa_K125010|the broad-host-range plasmid derived from RSF1010]]. The coding region was inserted into pSB1A3.

Notes

I am having trouble inserting this part into the Base Vector so the construct will be tested while still in pSB1A3.

Source

This part was derived from RSF1010 and RP4.

Components

Access	Instance	Definition
public public public public public public public public public	BBa_I14032 BBa_B0034 BBa_K125510 BBa_K125340 BBa_I14032 BBa_B0034 BBa_K125310 BBa_K125500 BBa_K125320	P(Lac) IQ BBa_B0034 Rep OriV P(Lac) IQ BBa_B0034 slr2016 SS BBa_K125500 OriT-RP4

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_I14032 BBa_B0034 BBa_K125510 BBa_K125340 BBa_I14032 BBa_B0034 BBa_K125310 BBa_K125500 BBa_K125320	1,37 46,57 66,3450 3459,3882 3891,3927 3936,3947 3956,4008 4017,4734 4743,4841	P(Lac) IQ BBa_B0034 Rep OriV P(Lac) IQ BBa_B0034 slr2016 SS BBa_K125500 OriT-RP4

Other Properties

igem#experience	None
igem#sampleStatus	It's complicated
igem#status	Planning
synbiohub#ownedBy	user/james
synbiohub#ownedBy	user/myers
synbiohub#topLevel	BBa_K125020/1