BBa_K1162014

BBa_K1162014 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1162014
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ryan Putman
Date created: 2013-09-16 11:00:00
Date modified: 2015-05-08 01:09:32

protmoter+rbs+N-terminal 10x His-Tag+GFP with no translational stop codon



    • Details

    Types
    DnaRegion

    Roles
    engineered_region

    Composite

    Sequences BBa_K1162014_sequence (Version 1)

    Description

    N-terminal 10x His-Tag fused with GFP (no translational stop codon) to allow for N-terminal HT-GFP tagged protein fusion. RFC 23 allows for in frame fusion on the C-terminal end of GFP.

    Notes

    RFC 23. ATG(Met) at start on coding region.

    Source

    GFP is synthetic form of GFP from a Jellyfish (Aequorea victoria).

    Access Instance Definition
    public
    public
    		BBa_K1162010
    BBa_K1162011
    		BBa_K1162010
    BBa_K1162011

    Sequence Annotation Location Component / Role(s)
    BBa_K1162010
    BBa_K1162011
    		1,259
    266,982
    		BBa_K1162010
    BBa_K1162011

    igem#sampleStatus
    It's complicated
    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K1162014/1
     

    Attachments

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