BBa_K113005

BBa_K113005 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K113005

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Wang Jinyu

Date created: 2008-10-25 11:00:00

Date modified: 2015-05-08 01:09:20

pLac->tetR->pT7->tetR operator ->GFP

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_K113005_sequence (Version 1)

Description

Whole construct of repressor test for right side. ---HKUSTers/2008

pLac + tetR + Terminator + pT7 + tetR operator + GFP + Terminator

The expression of tetR can be regulated by lac operon. When tetR is produced, it can bind to tetR operator so that the expression of GFP will be turned off. It is a test designed to check the effiency of the repressor tetR.

Notes

We combined BBa_K113003 and BBa_K113004 by fusion PCR. Since the efficency is very low, we tried to use standard assembly to ligate them together.

Source

BBa_K113003 BBa_K113004

Components

Access	Instance	Definition
public	BBa_K113003	BBa_K113003
public	BBa_B0034	BBa_B0034
public	BBa_B0010	BBa_B0010
public	BBa_B0012	BBa_B0012
public	BBa_K113004	BBa_K113004

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K113003	1,903	BBa_K113003
BBa_B0034	993,1004	BBa_B0034
BBa_B0010	1739,1818	BBa_B0010
BBa_B0012	1827,1867	BBa_B0012
BBa_K113004	912,1867	BBa_K113004

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K113005/1

Member of these Collections

• iGEM Parts Registry