View

BBa_J31012

BBa_J31012 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J31012

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Karmella Haynes

Date created: 2006-11-10 12:00:00

Date modified: 2015-08-31 04:08:45

Tet resistance expression cassette

Back

Details

Types	DnaRegion
Roles	engineered_region Reporter
Sequences	BBa_J31012_sequence (Version 1)

Description

Double terminator BBa_B0015 (TT) placed upstream of pBad, RBS and the Tet^R coding region. TT is placed upstream of the promoter to block read-through transcription. This cassette will be inserted into various plasmids of different copy numbers to gauge cell survival on increasing concentrations of tetracycline vs. the number of Tet+ plasmids in a cell.

Notes

HixC-pBad-HixC-RBS-TetF-HixC BBa_J44012 was cut with XbaI/ PstI and inserted to the right of double terminator BBa_B0015 (SpeI/ PstI).

Source

Double terminator BBa_B0015 and HixC-pBad-HixC-RBS-TetF-HixC BBa_J44012

Components

Access	Instance	Definition
public public public public public public public public	BBa_B0010 BBa_B0012 BBa_J44000 BBa_I13453 BBa_J44000 BBa_B0030 BBa_J31007 BBa_J44000	BBa_B0010 BBa_B0012 hixC BBa_I13453 hixC BBa_B0030 tetA(C)f hixC

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_B0010 BBa_B0012 BBa_J44000 BBa_I13453 BBa_J44000 BBa_B0030 BBa_J31007 BBa_J44000	1,80 89,129 138,163 172,301 310,335 344,358 365,1555 1564,1589	BBa_B0010 BBa_B0012 hixC BBa_I13453 hixC BBa_B0030 tetA(C)f hixC

Other Properties

igem#experience	Works
igem#sampleStatus	Not in stock
igem#status	Unavailable
synbiohub#ownedBy	user/james
synbiohub#ownedBy	user/myers
synbiohub#topLevel	BBa_J31012/1