Submit
Login or Register
View
HisTag
BBa_J18909
Version 1
Component
Source:
http://parts.igem.org/Part:BBa_J18909
Generated By:
https://synbiohub.org/public/igem/igem2sbol/1
Created by: Raik Gruenberg
Date created: 2010-01-15 12:00:00
Date modified: 2015-08-31 04:08:35
His tag (6xHis
E. coli - optimized)
Download
Download SBOL File
Download COMBINE Archive
Download GenBank
Download GFF3
Download FASTA
Search
Find Uses
Find Twins
Send
Send to Benchling
Send to ICE
Back
VisBOL
Zoom
Truncate Labels
PolyHis
Details
Types
DnaRegion
Roles
tag
Tag
Sequences
BBa_J18909_sequence (Version 1)
Description
Hexahistidine affinity tag.
Binds zinc, nickel, or cobalt affinity resins.
=Notes=
This part is identical to BBa_I757013 (which does not seem to be available though).
=References=
Wikipedia article:
http://en.wikipedia.org/wiki/Polyhistidine-tag
Notes
Simple repetition of the His codon most frequent in E. coli -- a more balanced codon-choice may be better.
Source
Synthetic DNA
Sequence Annotations
Sequence Annotation
Location
Component
/ Role(s)
PolyHis
1,18
tag
feature/tag
Other Properties
igem#experience
None
igem#sampleStatus
It's complicated
igem#status
Planning
synbiohub#ownedBy
user/james
synbiohub#ownedBy
user/myers
synbiohub#topLevel
BBa_J18909/1
Member of these Collections
//proteindomain/affinity
iGEM Parts Registry
Attachments
Sequence Visualization
bc0371da-68ed-4cd9-b538-67e6da90b424
Component Sankey
53107406-aaf2-405d-b76f-73b249dafeb0
Component Bar
91adbbe4-fac4-4fd6-a6d6-b23efc59e787