Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jesse Campbell, Brandon Grieshaber, Sara Pearson, Sachii Polpitya Arachchige
Date created: 2014-02-05 12:00:00
Date modified: 2015-08-31 04:08:28
Ammeline Biosensor with Riboswitch M6C (T92C)
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_J119337_sequence (Version 1) |
Description
The construct is a biosensor for ammeline. The P5 promoter drives a riboswitch (M6C from http://www.pnas.org/content/early/2010/01/21/0911209107) that allows translation in the presence of ammeline (made by demethylation of melamine) of a RFP reporter gene. Mutation of T to C at position 92 nt.Notes
None.Source
Mutagenesis of BBa_J119329 with inverse PCR followed by BsaI Golden Gate Assembly.| Sequence Annotation | Location | Component / Role(s) |
| P5 promoter M6C Ammeline riboswitch RFP T to C mutation | 19,55 73,188 183,866 92,92 | promoter feature/promoter feature/rbs ribosome_entry_site CDS feature/cds feature/mutation sequence_alteration |