| Types | DnaRegion
|
| Roles | promoter
Regulatory
|
Description
This promoter is found in parts in over 33 genes within E. coli. These genes contain the identifiers TATCCC ( ) GGATA, with the () containing any number of base pairs, dependent on which gene the promoter codes for. We chose to use the () specific to the mod F gene and test its effects on the pClone red gene within E. coli.
Notes
We added 1 base pair of G prior to the 3' to 5' sticky end so that the designated number of base pairs between the promoter and transcription initiation site would be met, as 5 were necessary and we only had 4 with the stick ends.
Source
http://www.karger.com/Article/FullText/354311
