BBa_J100092

BBa_J100092 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J100092

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Natalie Spach

Date created: 2012-09-12 11:00:00

Date modified: 2015-08-31 04:08:22

Constitutive promoter for M1-162

Details

• Types: DnaRegion
• Roles: Regulatory; promoter
• Sequences: BBa_J100092_sequence (Version 1)

Description

Constitutive promoter for M1-162

Notes

We had to cut out several sections of the promoter to make it smaller than 70 nucleotides (including the sticky ends).
We removed these segments of the promoter sequence: TGTACAGTACTTCAATTTGTTTAAAC, and CAGGAAACAGCT
These two sequences were associated with the ribosomal binding sites and the messenger RNA stabilizing regions, which were not essential to have in our promoter because the RFP gene (the gene our promoter is transcribing) contains all the necessary genetic information to undergo translation and transcription after transcription has been initiated.

Source

From lacZ gene, in lac operon from e. coli bacteria

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
-10	39,44	non_covalent_binding_site, feature/binding
-35	16,21	non_covalent_binding_site, feature/binding

Other Properties

• igem#experience: Works
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J100092/1

Member of these Collections

• iGEM Parts Registry