BBa_I766677

BBa_I766677 Version 1

Component

Source: http://parts.igem.org/Part:BBa_I766677

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Lauren Jann

Date created: 2007-08-15 11:00:00

Date modified: 2015-08-31 04:08:10

YopH-RR under strong constitutive promoter

Details

• Types: DnaRegion
• Roles: engineered_region; Generator
• Sequences: BBa_I766677_sequence (Version 1)

Description

This device will consititutively express YopH, a pathogen derived reversible inhibitor (phosphatase) of Map Kinase Fus3, under the pAdh promoter. The effector protein is fused to a RR zipper, and will be recruited to elements possessing a matching EE zipper. The effector is fused to mCherry (RFP) on the N-terminus.

Notes

Device was created using a method of combinatorial cloning using the type IIs restriction endonuclease AarI. Each device contains adaptor regions in between coding regions (RFP, effectors, and zippers) for this method.

Source

composite part

Components

Access	Instance	Definition
public	BBa_I766556	BBa_I766556
public	BBa_I766101	BBa_I766101
public	BBa_I766007	BBa_I766007
public	BBa_I766103	BBa_I766103
public	BBa_I766600	BBa_I766600

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_I766556	1,1501	BBa_I766556
BBa_I766101	1508,2227	BBa_I766101
BBa_I766007	2236,3648	BBa_I766007
BBa_I766103	3657,3818	BBa_I766103
BBa_I766600	3827,4044	BBa_I766600

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_I766677/1

Member of these Collections

• iGEM Parts Registry