BBa_I715050

BBa_I715050 Version 1

Component

This part has been discontinued.

Please consider using BBa_I715049 as a replacement.

Source:
http://parts.igem.org/Part:BBa_I715050
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Amanda Valencia
Date created: 2007-10-23 11:00:00
Date modified: 2015-08-31 04:07:50

Hin + pT7 -> RFP1 + GFP1 medium initial orientation



VisBOL
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Truncate Labels
hixCBBa_B0012BBa_B0010GFP2hixCBBa_B0012BBa_B0010RFP2hixCGFP1BBa_B0034RFP2hixCRFP1BBa_B0034BBa_R0085BBa_B0012BBa_B0010Hin LVABBa_B0030LacI
Types
DnaRegion

Roles
engineered_region

Device

Sequences BBa_I715050_sequence (Version 1)

Description

Part is composed of a Hin recombinase expression casette driven by pLac and series of flippable units that include halves of GFP and RFP: T7prom-RBS-RFP1-hixC-RFP2-RBS-GFP1-hixC-RFP2-TT-hixC-GFP2-TT-hixC. The corresponding part without Hin does not glow. With Hin, most colonies do not glow, but green and red colonies appear. Evidence suggests that this is a result of Hin-mediated recombination.

Notes

None

Source

Ligation of existing parts

igem#experience
None
 
igem#sampleStatus
Discontinued
igem#status
Deleted
 
isReplacedBy
BBa_I715049/1
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_I715050/1