Our terminator is based on strong, Rho-independent terminator of gyrase gene, which is type II topoisomerase (EC 5.99.1.3). DNA gyrase uses the energy of ATP hydrolysis to introduce negative supercoils or relaxes positive supercoils. Negative DNA supercoiling is essential in vivo to compact the genome, relieve torsional strain during replication, and promote local melting for vital processes such as transcript initation by RNA polymerase. Gyrase gene is an housekeeping gene and because of that it must have efficient terminator.
Terminator of gyrase (EC 5.99.1.3) gene.
T7 gene 1 specific transcriptional terminator
Extracted from genomic DNA of ''S. cerevisiae'' using PCR.
T1-motni, terminator for Aspergillus niger
T1-motni is terminator that works in the fungus Aspergillus niger
Compatible with the Plug'n'Play assembly standard
This is the B0010 part extracted by PCR from the doulbe terminator B0012. We didn't manage to extract it directly from the registry and we discovered that others had the same problem; for this reason we are submitting it once again, confirmed by sequencing and by experience...ready to use! The characterization has been done both with T7 and Coli RNApolymerases.
I don't have any design considerations, anyway it is useful to use TBE1X instead of TAE1X to make screening gels of such small fragments.
This part comes from B0012 contained in E0840, a double terminator in which B0010 is the second one. We used an extraction PCR.
Reverse of bi-directional terminator B0051. (E.coli derived, genbank U24206 bases 2694-2727. between converging yciA and tonA genes.)
Terminator to be placed downstream of multiple cloning site in new pSB series plasmids. Similar to BBa_B0051.
identical to E. coli MG1655 between tonB and yciA
Bidirectional transcriptional terminator cloned in the reverse direction of B0011. 22 bp stem-loop
Artificial terminator, estimated %T~=80% # 8bp stem, 6nt loop # Bidirectional, estimated reverse %T~>90%
Bidirectional, with the reverse estimated to be more effective than the forward. Has a polyA tail of 6 residues.
modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs
hGH poly A, terminator for mammalian cells
From plasmid N-terminal pFLAG-CMV from Sigma-Aldrich
Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 6 residues.
Strong transcriptional terminator consisting of a 20 bp stem-loop from Salmonella phage P22.
Artificial terminator, estimated %T~=55 * 6bp stem, 4nt loop * Bidirectional, estimated reverse %T~<20
Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 4 residues.
Reverse of B0053, E.coli transcriptional terminator from the His operon.
T3-motni, terminator for Aspergillus niger
T3-motni is a terminator for use in Aspergillus niger.
Transcription terminator for the E.coli RNA polymerase.
Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
This is the exact region between the end of the ORF of STE2-W and the beginning of BST1-C
This sequence was taken from the yeast genome
SV40 poly A, terminator for mammalian cells
SV40 poly A, terminator for use in mammalian cells
The lambda t1 is a strong transcriptional terminator with 99% efficiency in vivo.
Terminator in Bgl operon that is antiterminated by bglG
Artificial terminator, estimated %T~=70% *8bp stem, 6nt loop *Bidirectional, estimated reverse %T~=80%
Bidirectional, with the reverse estimated to be more effective than the forward. Has a polyA tail of 5 residues.
modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pair
trpC terminator for Aspergillus nidulans
TtrpC is a terminator of tryptophan synthase of Aspergillus nidulans.
From the plasmid p71 from the Center for Microbial Biotechnology at the Technical University of Denmark plasmid collection.
Artificial terminator, estimated %T~>90% * 8bp stem, 6nt loop * Bidirectional, estimated reverse %T~>90%
Bidirectional, with the reverse estimated to be more effective than the forward. Has a polyA tail of 4 residues
Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.
In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.
Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
Artificial terminator, estimated %T~<10% 8bp stem, 6nt loop Bidirectional, estimated reverse %T~=40%
Bidirectional, with the reverse estimated to be more effective than the forward. Has a polyA tail of 3 residues.
This part is the reverse of terminator B0013, a bacteriophage T7 terminator which was engineered to be bidirectional. Contains a poly A insertion upstream of a 20 bp stem-loop.
Note: The submitted version of this part has a mutation in the BioBrick suffix: The NdeI restriction site has been lost. However, the part can still be used, because the SpeI and PstI restriction sites are functional.
T7 terminator for protein expression in E. coli or in vitro. Part is provided in R.F.C. 25 assembly format.
* taken from pET3a expression plasmid * added two STOP to 5' * conservative choice of part boundary, could probably be shortened
Bi-directional Terminator derived from pBR322.
Tadashi Nakamura, Shuhei Yasumoto, Takahiro Saka, Saya Kakuda
Engineered 'cyc100 promoter' of Saccharomyces cerevisiae with Kozak sequence attached downstream.
Registry parts BBa_K105027 (promoter) and BBa_J63003 (Kozak).
Artificial terminator, estimated %T~=25% * 6bp stem, 4nt loop * Bidirectional, estimated reverse %T~<10%
Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 3 residues. Designed to be a terminator with low efficiency.
This part contains a bidirectional, high efficiency terminator, in the BioBytes universal plasmid pAB.See the BioBytes RFC for details on the BioBytes assembly method.
A terminator with long sticky ends can be prepared from this plasmid and assembled downstream of a gene using the Biobytes method. The addition of one DNA segment with the BioBytes method takes only 20 minutes. This ability to easily 'mix and match' terminators and genes allows rapid testing of different terminator designs.
The terminator is derived from part b1006. pAB is entered as part K187000.
Terminator from the E.coli ribosonal RNA rrnC operon. This terminator occurs in the reverse orientation upstream of the multiple cloning site of the pSB**1-pSB**3 series of plasmids.
The T0 transcriptional terminator terminates transcription with 80-90% efficiency.
eukaryotic -- derived from SV40 early poly A signal sequence
It is cloned in BioBrick vector pSB1AK3.
BGH poly A, terminator for use in mammalian cells
Reverse of B0052, E.coli transcriptional terminator from the ribosomal RNA rrnC operon.
Terminator of Yeast mitochondrial gene Q0255
This is the terminator region for Q0255 gene. It is selected from Yeast mito-chromosome, and we want to test it in our experiment.
This is obtained from Saccharomyces cerevisiae S288C mito-chromosome region. http://www.yeastgenome.org/
We modelled the RNA secondary structure of Q0255 3'UTR region, and found that the secondary structure of this region is very similar to bacterial terminator sequence.
Transcriptional terminator consisting of a 64 bp stem-loop (B0010), cloned onto the antisense strand.
Antiquity. thought to be T1 transcriptional terminator from E. coli RRNB operon.
Uni-directional terminator. Reverse of part B0012. 20 bp stem-loop.
Bi-directional Terminator from pSMART HCKAN. E.coli derived, genbank U24206 bases 2694-2727 between yciA and tonA genes (converging transcripts)
Artificial terminator, estimated %T~>90% *8bp stem, 6nt loop *Bidirectional, estimated reverse %T~>90%
Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 9 residues.
Terminator to be placed upstream of multiple cloning site in new pSB series plasmids.
Strong transcriptional terminator consisting of a 20 bp stem-loop that has been engineered to be bidirectional.
Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Subsequently mutations were introduced to make the terminator bi-directional (i.e., AAAAAA insertion on 5' side of the stem loop). Additional mutations were introduced on the 3' side of the stem loop to increase the number of T's and eliminate any promoter -10 site that might be present to avoid initiation of transcription of whatever is downstream. Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
trkD, a functional Kup (formerly TrkD) system took up Cs+ with a moderate rate and affinity
Escherichia coli cells which contain a functional Kup (formerly TrkD) system took up Cs' with a moderate rate and affinity. Kup is a separate K+ uptake system with relatively little discrimination in the transport of the cations K+, Rb+, and Cs'. Regardless of the presence or absence of Kup, K+-replete cells took up Cs'primarily by a very low affinity mode, proportional to the ratio of the Cs' and K+ concentrations in the medium. In our project, trkD is used to collect Cs-137 when recN is activated by radiation and start the transcription of trkD,in order to absorb this kind of radioactive source like Cs-137
Artifical terminator, estimated %T~=80 6bp stem, 4nt loop Bidirectional, estimated reverse %T=40
Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 5 residues.
T2-motni, terminator for Aspergillus niger
T2-motni is a terminator for use in Aspergillus niger
Transcriptional terminator consisting of a 64 bp stem-loop.
Terminator of Yeast mitochondrial gene COX3
This is the terminator region for COX3 gene. It is selected from Yeast mito-chromosome, and we want to test it in our experiment.
We modelled the RNA secondary structure of COX3 3'UTR region, and found that the secondary structure of this region is very similar to bacterial terminator sequence.
Reverse Bi-directional terminator from B0050 (derived from pBR322).