//s
type: Collection
persistent identity: https://synbiohub.org/public/igem/s
display id: s
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: JAMT
member: BAT2
member: EWOBS
member: BAT2/THI3
member: p(osmY)
member: BAMT
member: pchBA
member: Banana
member: BSMT1
member: 3-MBG
member: GFP genera
member: THI3
member: Wintergree
member: SG
member: BOBS
member: Exponentia
member: ATF1 gener
member: BBa_J45991
member: YYC912
member: SAMT
member: BOG
member: PchA/PchB
member: p(osmY).GF
member: ATF1
member: SBOBS
member: WOG
member: exp. GFP
member: WOBS

SAMT
type: ComponentDefinition
persistent identity: BBa J45001
display id: BBa_J45001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SAM:salicylic acid carboxyl methyltransferase; converts salicylic acid to methyl salicylate (winter
creator: Kate Broadbent
created: 2006-06-06T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _84_
m user id: 0
owner id: 976
owning group id: 84
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: From snapdragon (A. Majus)
mutable notes: lala
mutable provenance: Dutareva
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: double TAA stop codon
sequence annotation: start codon
sequence annotation: SAMT
sequence: https://synbiohub.org/public/igem/BBa_J45001_sequence/1

double TAA stop codon
type: SequenceAnnotation
persistent identity: annotation1880597
display id: annotation1880597
version: 1
top level: SAMT
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_J45001/annotation1880597/range1880597/1

https://synbiohub.org/public/igem/BBa_J45001/annotation1880597/range1880597/1
type: Range
persistent identity: range1880597
display id: range1880597
version: 1
direction: direction/forward
top level: SAMT
start: 1150
end: 1155
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation1880596
display id: annotation1880596
version: 1
top level: SAMT
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J45001/annotation1880596/range1880596/1

https://synbiohub.org/public/igem/BBa_J45001/annotation1880596/range1880596/1
type: Range
persistent identity: range1880596
display id: range1880596
version: 1
direction: direction/forward
top level: SAMT
start: 1
end: 3
orientation: inline

SAMT
type: SequenceAnnotation
persistent identity: annotation1880595
display id: annotation1880595
version: 1
top level: SAMT
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J45001/annotation1880595/range1880595/1

https://synbiohub.org/public/igem/BBa_J45001/annotation1880595/range1880595/1
type: Range
persistent identity: range1880595
display id: range1880595
version: 1
direction: direction/forward
top level: SAMT
start: 1
end: 1151
orientation: inline

THI3
type: ComponentDefinition
persistent identity: BBa J45009
display id: BBa_J45009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ketoisocaproate decarboxylase (THI3); converts alpha-ketoisocaproate to 3-methylbutanal
creator: Veena Venkatachalam
created: 2006-06-06T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _84_
m user id: 0
owner id: 642
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: dfsd
mutable notes: dfd
mutable provenance: dfd
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: THI3
sequence annotation: a->g to eliminate EcoRI, to do
sequence annotation: t->c to eliminate EcoRI, to do
sequence annotation: stop codon
sequence annotation: start codon
sequence annotation: t->c to eliminate SpeI, to do
sequence: https://synbiohub.org/public/igem/BBa_J45009_sequence/1

THI3
type: SequenceAnnotation
persistent identity: annotation1961298
display id: annotation1961298
version: 1
top level: THI3
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J45009/annotation1961298/range1961298/1

https://synbiohub.org/public/igem/BBa_J45009/annotation1961298/range1961298/1
type: Range
persistent identity: range1961298
display id: range1961298
version: 1
direction: direction/forward
top level: THI3
start: 1
end: 1830
orientation: inline

a->g to eliminate EcoRI, to do
type: SequenceAnnotation
persistent identity: annotation1891629
display id: annotation1891629
version: 1
top level: THI3
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_J45009/annotation1891629/range1891629/1

https://synbiohub.org/public/igem/BBa_J45009/annotation1891629/range1891629/1
type: Range
persistent identity: range1891629
display id: range1891629
version: 1
direction: direction/forward
top level: THI3
start: 906
end: 906
orientation: inline

t->c to eliminate EcoRI, to do
type: SequenceAnnotation
persistent identity: annotation1891628
display id: annotation1891628
version: 1
top level: THI3
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_J45009/annotation1891628/range1891628/1

https://synbiohub.org/public/igem/BBa_J45009/annotation1891628/range1891628/1
type: Range
persistent identity: range1891628
display id: range1891628
version: 1
direction: direction/forward
top level: THI3
start: 6
end: 6
orientation: inline

a->g to eliminate EcoRI, to do
type: SequenceAnnotation
persistent identity: annotation1891630
display id: annotation1891630
version: 1
top level: THI3
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_J45009/annotation1891630/range1891630/1

https://synbiohub.org/public/igem/BBa_J45009/annotation1891630/range1891630/1
type: Range
persistent identity: range1891630
display id: range1891630
version: 1
direction: direction/forward
top level: THI3
start: 1605
end: 1605
orientation: inline

stop codon
type: SequenceAnnotation
persistent identity: annotation1961304
display id: annotation1961304
version: 1
top level: THI3
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_J45009/annotation1961304/range1961304/1

https://synbiohub.org/public/igem/BBa_J45009/annotation1961304/range1961304/1
type: Range
persistent identity: range1961304
display id: range1961304
version: 1
direction: direction/forward
top level: THI3
start: 1828
end: 1830
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation1961303
display id: annotation1961303
version: 1
top level: THI3
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J45009/annotation1961303/range1961303/1

https://synbiohub.org/public/igem/BBa_J45009/annotation1961303/range1961303/1
type: Range
persistent identity: range1961303
display id: range1961303
version: 1
direction: direction/forward
top level: THI3
start: 1
end: 3
orientation: inline

a->g to eliminate EcoRI, to do
type: SequenceAnnotation
persistent identity: annotation1891631
display id: annotation1891631
version: 1
top level: THI3
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_J45009/annotation1891631/range1891631/1

https://synbiohub.org/public/igem/BBa_J45009/annotation1891631/range1891631/1
type: Range
persistent identity: range1891631
display id: range1891631
version: 1
direction: direction/forward
top level: THI3
start: 1791
end: 1791
orientation: inline

t->c to eliminate SpeI, to do
type: SequenceAnnotation
persistent identity: annotation1891632
display id: annotation1891632
version: 1
top level: THI3
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_J45009/annotation1891632/range1891632/1

https://synbiohub.org/public/igem/BBa_J45009/annotation1891632/range1891632/1
type: Range
persistent identity: range1891632
display id: range1891632
version: 1
direction: direction/forward
top level: THI3
start: 699
end: 699
orientation: inline

3-MBG
type: ComponentDefinition
persistent identity: BBa J45400
display id: BBa_J45400
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 3-methylbutanal generator
creator: MIT IGEM 2006
created: 2006-08-20T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _84_
m user id: 0
owner id: 1147
owning group id: 84
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This device will make isoamyl alcohol. It codes for an enzymatic pathway that takes in leucine and converts it to isoamyl alcohol.
mutable notes: Out of time constraints, we did not get rid of the EcoRI cut sites in THI3. So...don't use EcoRI when making assemblies with this part!
mutable provenance: The coding regions (BAT2 and THI3) in this part came from S. cerevisae.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_J45008
component: BBa_B0030
component: BBa_R0011
component: BBa_J45009
sequence annotation: BBa_J45008
sequence annotation: BBa_B0030
sequence annotation: BBa_R0011
sequence annotation: BBa_J45009
sequence: https://synbiohub.org/public/igem/BBa_J45400_sequence/1

BBa_J45008
type: Component
persistent identity: component1960893
display id: component1960893
version: 1
top level: 3-MBG
access: public
definition: BAT2

BBa_B0030
type: Component
persistent identity: component1960888
display id: component1960888
version: 1
top level: 3-MBG
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_R0011
type: Component
persistent identity: component1960882
display id: component1960882
version: 1
top level: 3-MBG
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_J45009
type: Component
persistent identity: component1960902
display id: component1960902
version: 1
top level: 3-MBG
access: public
definition: THI3

BBa_B0030
type: Component
persistent identity: component1960895
display id: component1960895
version: 1
top level: 3-MBG
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_J45008
type: SequenceAnnotation
persistent identity: annotation1960893
display id: annotation1960893
version: 1
top level: 3-MBG
location: https://synbiohub.org/public/igem/BBa_J45400/annotation1960893/range1960893/1
component: BBa_J45008

https://synbiohub.org/public/igem/BBa_J45400/annotation1960893/range1960893/1
type: Range
persistent identity: range1960893
display id: range1960893
version: 1
direction: direction/reverse
top level: 3-MBG
start: 85
end: 1218
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1960888
display id: annotation1960888
version: 1
top level: 3-MBG
location: https://synbiohub.org/public/igem/BBa_J45400/annotation1960888/range1960888/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_J45400/annotation1960888/range1960888/1
type: Range
persistent identity: range1960888
display id: range1960888
version: 1
direction: direction/forward
top level: 3-MBG
start: 64
end: 78
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation1960882
display id: annotation1960882
version: 1
top level: 3-MBG
location: https://synbiohub.org/public/igem/BBa_J45400/annotation1960882/range1960882/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_J45400/annotation1960882/range1960882/1
type: Range
persistent identity: range1960882
display id: range1960882
version: 1
direction: direction/forward
top level: 3-MBG
start: 1
end: 54
orientation: inline

BBa_J45009
type: SequenceAnnotation
persistent identity: annotation1960902
display id: annotation1960902
version: 1
top level: 3-MBG
location: https://synbiohub.org/public/igem/BBa_J45400/annotation1960902/range1960902/1
component: BBa_J45009

https://synbiohub.org/public/igem/BBa_J45400/annotation1960902/range1960902/1
type: Range
persistent identity: range1960902
display id: range1960902
version: 1
direction: direction/reverse
top level: 3-MBG
start: 1248
end: 3077
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1960895
display id: annotation1960895
version: 1
top level: 3-MBG
location: https://synbiohub.org/public/igem/BBa_J45400/annotation1960895/range1960895/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_J45400/annotation1960895/range1960895/1
type: Range
persistent identity: range1960895
display id: range1960895
version: 1
direction: direction/forward
top level: 3-MBG
start: 1227
end: 1241
orientation: inline

WOG
type: ComponentDefinition
persistent identity: BBa J45120
display id: BBa_J45120
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Wintergreen odor generator
creator: MIT IGEM 2006
created: 2006-08-09T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _84_
m user id: 0
owner id: 1147
owning group id: 84
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is the wintergreen generating device hooked up to a constitutive promoter with a different RBS (B0032, different from 45100).
mutable notes: N/A
mutable provenance: Built off biobricks R0040 and 45119.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_J45004
component: BBa_B0010
component: BBa_R0040
component: BBa_B0012
component: BBa_B0032
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_B0032
sequence annotation: BBa_J45004
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_J45120_sequence/1

BBa_J45004
type: Component
persistent identity: component1894776
display id: component1894776
version: 1
top level: WOG
access: public
definition: BSMT1

BBa_B0010
type: Component
persistent identity: component1894777
display id: component1894777
version: 1
top level: WOG
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0040
type: Component
persistent identity: component1894766
display id: component1894766
version: 1
top level: WOG
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0012
type: Component
persistent identity: component1894779
display id: component1894779
version: 1
top level: WOG
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0032
type: Component
persistent identity: component1894771
display id: component1894771
version: 1
top level: WOG
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1894777
display id: annotation1894777
version: 1
top level: WOG
location: https://synbiohub.org/public/igem/BBa_J45120/annotation1894777/range1894777/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J45120/annotation1894777/range1894777/1
type: Range
persistent identity: range1894777
display id: range1894777
version: 1
direction: direction/forward
top level: WOG
start: 1164
end: 1243
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1894779
display id: annotation1894779
version: 1
top level: WOG
location: https://synbiohub.org/public/igem/BBa_J45120/annotation1894779/range1894779/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J45120/annotation1894779/range1894779/1
type: Range
persistent identity: range1894779
display id: range1894779
version: 1
direction: direction/forward
top level: WOG
start: 1252
end: 1292
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1894771
display id: annotation1894771
version: 1
top level: WOG
location: https://synbiohub.org/public/igem/BBa_J45120/annotation1894771/range1894771/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_J45120/annotation1894771/range1894771/1
type: Range
persistent identity: range1894771
display id: range1894771
version: 1
direction: direction/forward
top level: WOG
start: 63
end: 75
orientation: inline

BBa_J45004
type: SequenceAnnotation
persistent identity: annotation1894776
display id: annotation1894776
version: 1
top level: WOG
location: https://synbiohub.org/public/igem/BBa_J45120/annotation1894776/range1894776/1
component: BBa_J45004

https://synbiohub.org/public/igem/BBa_J45120/annotation1894776/range1894776/1
type: Range
persistent identity: range1894776
display id: range1894776
version: 1
direction: direction/reverse
top level: WOG
start: 82
end: 1155
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1894766
display id: annotation1894766
version: 1
top level: WOG
location: https://synbiohub.org/public/igem/BBa_J45120/annotation1894766/range1894766/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_J45120/annotation1894766/range1894766/1
type: Range
persistent identity: range1894766
display id: range1894766
version: 1
direction: direction/forward
top level: WOG
start: 1
end: 54
orientation: inline

PchA/PchB
type: ComponentDefinition
persistent identity: BBa J45319
display id: BBa_J45319
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PchA & PchB enzyme generator
creator: Andr?? Green II
created: 2006-10-27T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _84_
m user id: 0
owner id: 977
owning group id: 84
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This part is an intermediate to the protein generator BBa_45300. This part is almost identical to BBa_J45299 except that is uses as its RBS B0032 instead of B0030.
mutable notes: coming
mutable provenance: coming
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0032
component: BBa_J45017
component: BBa_B0010
component: BBa_B0012
sequence annotation: BBa_B0032
sequence annotation: BBa_B0010
sequence annotation: BBa_J45017
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_J45319_sequence/1

BBa_B0032
type: Component
persistent identity: component1960931
display id: component1960931
version: 1
top level: PchA/PchB
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_J45017
type: Component
persistent identity: component1960938
display id: component1960938
version: 1
top level: PchA/PchB
access: public
definition: pchBA

BBa_B0010
type: Component
persistent identity: component1960939
display id: component1960939
version: 1
top level: PchA/PchB
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1960941
display id: component1960941
version: 1
top level: PchA/PchB
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1960931
display id: annotation1960931
version: 1
top level: PchA/PchB
location: https://synbiohub.org/public/igem/BBa_J45319/annotation1960931/range1960931/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_J45319/annotation1960931/range1960931/1
type: Range
persistent identity: range1960931
display id: range1960931
version: 1
direction: direction/forward
top level: PchA/PchB
start: 1
end: 13
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1960939
display id: annotation1960939
version: 1
top level: PchA/PchB
location: https://synbiohub.org/public/igem/BBa_J45319/annotation1960939/range1960939/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J45319/annotation1960939/range1960939/1
type: Range
persistent identity: range1960939
display id: range1960939
version: 1
direction: direction/forward
top level: PchA/PchB
start: 1764
end: 1843
orientation: inline

BBa_J45017
type: SequenceAnnotation
persistent identity: annotation1960938
display id: annotation1960938
version: 1
top level: PchA/PchB
location: https://synbiohub.org/public/igem/BBa_J45319/annotation1960938/range1960938/1
component: BBa_J45017

https://synbiohub.org/public/igem/BBa_J45319/annotation1960938/range1960938/1
type: Range
persistent identity: range1960938
display id: range1960938
version: 1
direction: direction/reverse
top level: PchA/PchB
start: 20
end: 1755
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1960941
display id: annotation1960941
version: 1
top level: PchA/PchB
location: https://synbiohub.org/public/igem/BBa_J45319/annotation1960941/range1960941/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J45319/annotation1960941/range1960941/1
type: Range
persistent identity: range1960941
display id: range1960941
version: 1
direction: direction/forward
top level: PchA/PchB
start: 1852
end: 1892
orientation: inline

JAMT
type: ComponentDefinition
persistent identity: BBa J45003
display id: BBa_J45003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SAM:jasmonic acid carboxyl methyltransferase; converts jasmonic acid to jasmine odor
creator: Stephen Payne
created: 2006-06-06T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: true
experience: experience/None
group u list: _84_
m user id: 0
owner id: 642
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: This ORF encodes the enzyme responsible for catalyzing the following reaction: Jasmonic Acid--->Methyl Jasmonate It is initially found in ''Arabidopsis thaliana'' but has been transferred successfully to the E. coli genome by Seo, et al. The product, methyl jasmonate has a jasmine smell to it.
mutable notes: Km: 38.5 ??M; Kcat:25 S−1 for Jasmonic Acid--->Methyl Jasmonate with the aid of JAMT: Jasmonic acid carboxyl methyltransferase
mutable provenance: If the part becomes physically available, it would probably be donated by Seo, et al.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start codon
sequence annotation: stop codon
sequence annotation: JAMT
sequence: https://synbiohub.org/public/igem/BBa_J45003_sequence/1

start codon
type: SequenceAnnotation
persistent identity: annotation1961373
display id: annotation1961373
version: 1
top level: JAMT
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J45003/annotation1961373/range1961373/1

https://synbiohub.org/public/igem/BBa_J45003/annotation1961373/range1961373/1
type: Range
persistent identity: range1961373
display id: range1961373
version: 1
direction: direction/forward
top level: JAMT
start: 1
end: 3
orientation: inline

stop codon
type: SequenceAnnotation
persistent identity: annotation1961374
display id: annotation1961374
version: 1
top level: JAMT
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_J45003/annotation1961374/range1961374/1

https://synbiohub.org/public/igem/BBa_J45003/annotation1961374/range1961374/1
type: Range
persistent identity: range1961374
display id: range1961374
version: 1
direction: direction/forward
top level: JAMT
start: 1168
end: 1170
orientation: inline

JAMT
type: SequenceAnnotation
persistent identity: annotation1961372
display id: annotation1961372
version: 1
top level: JAMT
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J45003/annotation1961372/range1961372/1

https://synbiohub.org/public/igem/BBa_J45003/annotation1961372/range1961372/1
type: Range
persistent identity: range1961372
display id: range1961372
version: 1
direction: direction/forward
top level: JAMT
start: 1
end: 1168
orientation: inline

p(osmY).GF
type: ComponentDefinition
persistent identity: BBa J45995
display id: BBa_J45995
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Stationary phase dependent GFP generator
creator: Stephen Payne
created: 2006-10-30T12:00:00Z
modified: 2015-08-31T04:08:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _84_
m user id: 0
owner id: 642
owning group id: 84
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part is a composite part consisting of <biobrick>J45992</biobrick/> (a stationary phase promoter) and <biobrick>E0840</biobrick/> (a GFP generator). Thus, this device creates green fluorescent protein in stationary phase.
mutable notes: This part was mainly designed to test whether J45250 would smell like banana only in stationary phase.
mutable provenance: J45992 was PCRed out of the E. coli genome, while E0840 was constructed at the Registry of Standard Biological Parts.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_J45992
component: BBa_B0010
component: BBa_B0030
component: BBa_E0040
component: BBa_B0012
sequence annotation: BBa_B0012
sequence annotation: BBa_J45992
sequence annotation: BBa_E0040
sequence annotation: BBa_B0030
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_J45995_sequence/1

BBa_J45992
type: Component
persistent identity: component1909181
display id: component1909181
version: 1
top level: p(osmY).GF
access: public
definition: p(osmY)

BBa_B0010
type: Component
persistent identity: component1909186
display id: component1909186
version: 1
top level: p(osmY).GF
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0030
type: Component
persistent identity: component1909183
display id: component1909183
version: 1
top level: p(osmY).GF
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_E0040
type: Component
persistent identity: component1909185
display id: component1909185
version: 1
top level: p(osmY).GF
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0012
type: Component
persistent identity: component1909188
display id: component1909188
version: 1
top level: p(osmY).GF
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1909188
display id: annotation1909188
version: 1
top level: p(osmY).GF
location: https://synbiohub.org/public/igem/BBa_J45995/annotation1909188/range1909188/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J45995/annotation1909188/range1909188/1
type: Range
persistent identity: range1909188
display id: range1909188
version: 1
direction: direction/forward
top level: p(osmY).GF
start: 1045
end: 1085
orientation: inline

BBa_J45992
type: SequenceAnnotation
persistent identity: annotation1909181
display id: annotation1909181
version: 1
top level: p(osmY).GF
location: https://synbiohub.org/public/igem/BBa_J45995/annotation1909181/range1909181/1
component: BBa_J45992

https://synbiohub.org/public/igem/BBa_J45995/annotation1909181/range1909181/1
type: Range
persistent identity: range1909181
display id: range1909181
version: 1
direction: direction/reverse
top level: p(osmY).GF
start: 1
end: 199
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1909185
display id: annotation1909185
version: 1
top level: p(osmY).GF
location: https://synbiohub.org/public/igem/BBa_J45995/annotation1909185/range1909185/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_J45995/annotation1909185/range1909185/1
type: Range
persistent identity: range1909185
display id: range1909185
version: 1
direction: direction/reverse
top level: p(osmY).GF
start: 229
end: 948
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1909183
display id: annotation1909183
version: 1
top level: p(osmY).GF
location: https://synbiohub.org/public/igem/BBa_J45995/annotation1909183/range1909183/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_J45995/annotation1909183/range1909183/1
type: Range
persistent identity: range1909183
display id: range1909183
version: 1
direction: direction/forward
top level: p(osmY).GF
start: 208
end: 222
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1909186
display id: annotation1909186
version: 1
top level: p(osmY).GF
location: https://synbiohub.org/public/igem/BBa_J45995/annotation1909186/range1909186/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J45995/annotation1909186/range1909186/1
type: Range
persistent identity: range1909186
display id: range1909186
version: 1
direction: direction/forward
top level: p(osmY).GF
start: 957
end: 1036
orientation: inline

Exponentia
type: ComponentDefinition
persistent identity: BBa J45994
display id: BBa_J45994
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Exponential phase transcriptional control device
creator: Reshma Shetty
created: 2008-01-29T12:00:00Z
modified: 2015-08-31T04:08:50Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _84_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: This composite regulator device produces a transcriptional signal in early growth phase but not in stationary phase.
mutable notes: None.
mutable provenance: It is a composite part of <partinfo>BBa_J45992</partinfo> and <partinfo>BBa_Q04401</partinfo>.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_Q04401
component: BBa_J45992
sequence annotation: BBa_J45992
sequence annotation: BBa_Q04401
sequence: https://synbiohub.org/public/igem/BBa_J45994_sequence/1

BBa_Q04401
type: Component
persistent identity: component1960526
display id: component1960526
version: 1
top level: Exponentia
access: public
definition: https://synbiohub.org/public/igem/BBa_Q04401/1

BBa_J45992
type: Component
persistent identity: component1960525
display id: component1960525
version: 1
top level: Exponentia
access: public
definition: p(osmY)

BBa_J45992
type: SequenceAnnotation
persistent identity: annotation1960525
display id: annotation1960525
version: 1
top level: Exponentia
location: https://synbiohub.org/public/igem/BBa_J45994/annotation1960525/range1960525/1
component: BBa_J45992

https://synbiohub.org/public/igem/BBa_J45994/annotation1960525/range1960525/1
type: Range
persistent identity: range1960525
display id: range1960525
version: 1
direction: direction/reverse
top level: Exponentia
start: 1
end: 199
orientation: inline

BBa_Q04401
type: SequenceAnnotation
persistent identity: annotation1960526
display id: annotation1960526
version: 1
top level: Exponentia
location: https://synbiohub.org/public/igem/BBa_J45994/annotation1960526/range1960526/1
component: BBa_Q04401

https://synbiohub.org/public/igem/BBa_J45994/annotation1960526/range1960526/1
type: Range
persistent identity: range1960526
display id: range1960526
version: 1
direction: direction/reverse
top level: Exponentia
start: 208
end: 1109
orientation: inline

EWOBS
type: ComponentDefinition
persistent identity: BBa J45500
display id: BBa_J45500
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Exponential phase wintergreen odor biosynthetic system
creator: MIT iGEM 2006
created: 2006-06-19T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_84_
m user id: 0
owner id: 2
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: prkA from Synechococcus PCC 7942
mutable notes: ...
mutable provenance: NCBI
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_J45320
component: BBa_J45181
sequence annotation: BBa_J45181
sequence annotation: BBa_J45320
sequence: https://synbiohub.org/public/igem/BBa_J45500_sequence/1

BBa_J45320
type: Component
persistent identity: component2291006
display id: component2291006
version: 1
top level: EWOBS
access: public
definition: SG

BBa_J45181
type: Component
persistent identity: component2291026
display id: component2291026
version: 1
top level: EWOBS
access: public
definition: Wintergree

BBa_J45181
type: SequenceAnnotation
persistent identity: annotation2291026
display id: annotation2291026
version: 1
top level: EWOBS
location: https://synbiohub.org/public/igem/BBa_J45500/annotation2291026/range2291026/1
component: BBa_J45181

https://synbiohub.org/public/igem/BBa_J45500/annotation2291026/range2291026/1
type: Range
persistent identity: range2291026
display id: range2291026
version: 1
direction: direction/reverse
top level: EWOBS
start: 1964
end: 4310
orientation: inline

BBa_J45320
type: SequenceAnnotation
persistent identity: annotation2291006
display id: annotation2291006
version: 1
top level: EWOBS
location: https://synbiohub.org/public/igem/BBa_J45500/annotation2291006/range2291006/1
component: BBa_J45320

https://synbiohub.org/public/igem/BBa_J45500/annotation2291006/range2291006/1
type: Range
persistent identity: range2291006
display id: range2291006
version: 1
direction: direction/reverse
top level: EWOBS
start: 1
end: 1955
orientation: inline

BAMT
type: ComponentDefinition
persistent identity: BBa J45002
display id: BBa_J45002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SAM:benzoic acid carboxyl methyltransferase; converts benzoic acid to methyl benzoate (floral odor)
creator: Andr?? Green II
created: 2006-06-06T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _84_
m user id: 0
owner id: 977
owning group id: 84
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: BAMT catalyzes the transfer of a methyl group from SAM (S-adenosyl methyltransferase) to benzoic acid, creating methyl benzoate. Methyl benzoate produces a "pleasant" smell.
mutable notes: none as of yet
mutable provenance: Genbank accession number(s): AF198492 This part is from A. majus.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: putative start codon 1
sequence annotation: double TAA stop codon
sequence annotation: BAMT
sequence annotation: putative start codon 2
sequence annotation: A -> G to eliminate SpeI site
sequence: https://synbiohub.org/public/igem/BBa_J45002_sequence/1

putative start codon 1
type: SequenceAnnotation
persistent identity: annotation1880475
display id: annotation1880475
version: 1
top level: BAMT
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J45002/annotation1880475/range1880475/1

https://synbiohub.org/public/igem/BBa_J45002/annotation1880475/range1880475/1
type: Range
persistent identity: range1880475
display id: range1880475
version: 1
direction: direction/forward
top level: BAMT
start: 1
end: 3
orientation: inline

double TAA stop codon
type: SequenceAnnotation
persistent identity: annotation1880495
display id: annotation1880495
version: 1
top level: BAMT
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_J45002/annotation1880495/range1880495/1

https://synbiohub.org/public/igem/BBa_J45002/annotation1880495/range1880495/1
type: Range
persistent identity: range1880495
display id: range1880495
version: 1
direction: direction/forward
top level: BAMT
start: 1093
end: 1098
orientation: inline

BAMT
type: SequenceAnnotation
persistent identity: annotation1880494
display id: annotation1880494
version: 1
top level: BAMT
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J45002/annotation1880494/range1880494/1

https://synbiohub.org/public/igem/BBa_J45002/annotation1880494/range1880494/1
type: Range
persistent identity: range1880494
display id: range1880494
version: 1
direction: direction/forward
top level: BAMT
start: 1
end: 1095
orientation: inline

putative start codon 2
type: SequenceAnnotation
persistent identity: annotation1880476
display id: annotation1880476
version: 1
top level: BAMT
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J45002/annotation1880476/range1880476/1

https://synbiohub.org/public/igem/BBa_J45002/annotation1880476/range1880476/1
type: Range
persistent identity: range1880476
display id: range1880476
version: 1
direction: direction/forward
top level: BAMT
start: 10
end: 12
orientation: inline

A -> G to eliminate SpeI site
type: SequenceAnnotation
persistent identity: annotation1880506
display id: annotation1880506
version: 1
top level: BAMT
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_J45002/annotation1880506/range1880506/1

https://synbiohub.org/public/igem/BBa_J45002/annotation1880506/range1880506/1
type: Range
persistent identity: range1880506
display id: range1880506
version: 1
direction: direction/forward
top level: BAMT
start: 702
end: 702
orientation: inline

BOG
type: ComponentDefinition
persistent identity: BBa J45250
display id: BBa_J45250
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Stationary-phase-dependent banana odor generator
creator: Andr?? Green II
created: 2006-10-27T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _84_
m user id: 0
owner id: 977
owning group id: 84
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part is an ATF1-1148 protein generator under control by the osmY stationary phase promoter. It is almost identical to J45270, except that: 1) The promoter is the truncated version of osmY and 2) it uses the B0030 RBS instead of B0032 (which is in J45270).
mutable notes: coming
mutable provenance: coming
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_J45992
component: BBa_B0010
component: BBa_B0030
component: BBa_B0012
component: BBa_J45014
sequence annotation: BBa_B0010
sequence annotation: BBa_J45014
sequence annotation: BBa_B0030
sequence annotation: BBa_J45992
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_J45250_sequence/1

BBa_J45992
type: Component
persistent identity: component1908072
display id: component1908072
version: 1
top level: BOG
access: public
definition: p(osmY)

BBa_B0010
type: Component
persistent identity: component1908081
display id: component1908081
version: 1
top level: BOG
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0030
type: Component
persistent identity: component1908074
display id: component1908074
version: 1
top level: BOG
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_B0012
type: Component
persistent identity: component1908083
display id: component1908083
version: 1
top level: BOG
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_J45014
type: Component
persistent identity: component1908080
display id: component1908080
version: 1
top level: BOG
access: public
definition: ATF1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1908081
display id: annotation1908081
version: 1
top level: BOG
location: https://synbiohub.org/public/igem/BBa_J45250/annotation1908081/range1908081/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J45250/annotation1908081/range1908081/1
type: Range
persistent identity: range1908081
display id: range1908081
version: 1
direction: direction/forward
top level: BOG
start: 1818
end: 1897
orientation: inline

BBa_J45014
type: SequenceAnnotation
persistent identity: annotation1908080
display id: annotation1908080
version: 1
top level: BOG
location: https://synbiohub.org/public/igem/BBa_J45250/annotation1908080/range1908080/1
component: BBa_J45014

https://synbiohub.org/public/igem/BBa_J45250/annotation1908080/range1908080/1
type: Range
persistent identity: range1908080
display id: range1908080
version: 1
direction: direction/reverse
top level: BOG
start: 229
end: 1809
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1908074
display id: annotation1908074
version: 1
top level: BOG
location: https://synbiohub.org/public/igem/BBa_J45250/annotation1908074/range1908074/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_J45250/annotation1908074/range1908074/1
type: Range
persistent identity: range1908074
display id: range1908074
version: 1
direction: direction/forward
top level: BOG
start: 208
end: 222
orientation: inline

BBa_J45992
type: SequenceAnnotation
persistent identity: annotation1908072
display id: annotation1908072
version: 1
top level: BOG
location: https://synbiohub.org/public/igem/BBa_J45250/annotation1908072/range1908072/1
component: BBa_J45992

https://synbiohub.org/public/igem/BBa_J45250/annotation1908072/range1908072/1
type: Range
persistent identity: range1908072
display id: range1908072
version: 1
direction: direction/reverse
top level: BOG
start: 1
end: 199
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1908083
display id: annotation1908083
version: 1
top level: BOG
location: https://synbiohub.org/public/igem/BBa_J45250/annotation1908083/range1908083/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J45250/annotation1908083/range1908083/1
type: Range
persistent identity: range1908083
display id: range1908083
version: 1
direction: direction/forward
top level: BOG
start: 1906
end: 1946
orientation: inline

BSMT1
type: ComponentDefinition
persistent identity: BBa J45004
display id: BBa_J45004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SAM:benzoic acid/salicylic acid carboxyl methyltransferase I; converts salicylic acid to methyl sali
creator: Boyuan Zhu
created: 2006-06-06T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _84_
m user id: 0
owner id: 974
owning group id: 84
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Codes for BSMT enzyme to convert Benzoic acid/salicylic acid to methyl benzoate and methyl salicylate. This part is phBSMT1 from Petunia x hybrida.
mutable notes: Transport issues are being considered.
mutable provenance: Species: Petunia x hybrida from Genbank; (also available: A. thaliana and N. suaveolens)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start codon
sequence annotation: BMST1
sequence annotation: stop codon
sequence: https://synbiohub.org/public/igem/BBa_J45004_sequence/1

start codon
type: SequenceAnnotation
persistent identity: annotation1880519
display id: annotation1880519
version: 1
top level: BSMT1
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J45004/annotation1880519/range1880519/1

https://synbiohub.org/public/igem/BBa_J45004/annotation1880519/range1880519/1
type: Range
persistent identity: range1880519
display id: range1880519
version: 1
direction: direction/forward
top level: BSMT1
start: 1
end: 3
orientation: inline

BMST1
type: SequenceAnnotation
persistent identity: annotation1880517
display id: annotation1880517
version: 1
top level: BSMT1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J45004/annotation1880517/range1880517/1

https://synbiohub.org/public/igem/BBa_J45004/annotation1880517/range1880517/1
type: Range
persistent identity: range1880517
display id: range1880517
version: 1
direction: direction/forward
top level: BSMT1
start: 1
end: 1074
orientation: inline

stop codon
type: SequenceAnnotation
persistent identity: annotation1880520
display id: annotation1880520
version: 1
top level: BSMT1
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_J45004/annotation1880520/range1880520/1

https://synbiohub.org/public/igem/BBa_J45004/annotation1880520/range1880520/1
type: Range
persistent identity: range1880520
display id: range1880520
version: 1
direction: direction/forward
top level: BSMT1
start: 1072
end: 1074
orientation: inline

GFP genera
type: ComponentDefinition
persistent identity: BBa E0840
display id: BBa_E0840
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP generator
creator: Jennifer Braff
created: 2004-10-17T11:00:00Z
modified: 2015-08-31T04:07:26Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: B0030.E0040.B0015
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0010
component: BBa_B0030
component: BBa_E0040
component: BBa_B0012
sequence annotation: BBa_B0030
sequence annotation: BBa_E0040
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_E0840_sequence/1

BBa_B0010
type: Component
persistent identity: component1249247
display id: component1249247
version: 1
top level: GFP genera
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0030
type: Component
persistent identity: component1249239
display id: component1249239
version: 1
top level: GFP genera
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_E0040
type: Component
persistent identity: component1249242
display id: component1249242
version: 1
top level: GFP genera
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0012
type: Component
persistent identity: component1249257
display id: component1249257
version: 1
top level: GFP genera
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1249239
display id: annotation1249239
version: 1
top level: GFP genera
location: https://synbiohub.org/public/igem/BBa_E0840/annotation1249239/range1249239/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_E0840/annotation1249239/range1249239/1
type: Range
persistent identity: range1249239
display id: range1249239
version: 1
direction: direction/forward
top level: GFP genera
start: 1
end: 15
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1249242
display id: annotation1249242
version: 1
top level: GFP genera
location: https://synbiohub.org/public/igem/BBa_E0840/annotation1249242/range1249242/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_E0840/annotation1249242/range1249242/1
type: Range
persistent identity: range1249242
display id: range1249242
version: 1
direction: direction/forward
top level: GFP genera
start: 22
end: 741
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1249257
display id: annotation1249257
version: 1
top level: GFP genera
location: https://synbiohub.org/public/igem/BBa_E0840/annotation1249257/range1249257/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_E0840/annotation1249257/range1249257/1
type: Range
persistent identity: range1249257
display id: range1249257
version: 1
direction: direction/forward
top level: GFP genera
start: 838
end: 878
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1249247
display id: annotation1249247
version: 1
top level: GFP genera
location: https://synbiohub.org/public/igem/BBa_E0840/annotation1249247/range1249247/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_E0840/annotation1249247/range1249247/1
type: Range
persistent identity: range1249247
display id: range1249247
version: 1
direction: direction/forward
top level: GFP genera
start: 750
end: 829
orientation: inline

EWOBS
type: ComponentDefinition
persistent identity: BBa J45800
display id: BBa_J45800
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Exponential phase wintergreen odor biosynthetic system
creator: MIT iGEM 2006
created: 2006-10-31T12:00:00Z
modified: 2015-08-31T04:08:50Z
discontinued: false
dominant: false
experience: experience/None
group u list: _84_
m user id: 0
owner id: 2
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: This system designed by the MIT 2006 iGEM team generates mint smell by biosynthesizing salicylic acid from chassis-produced metabolites (chorismate) and then converts salicylic acid into methyl salicylate (wintergreen scent). It uses a stationary phase promoter followed by an inverter (see <bbpart>BBa_J45996<bbpart> for details) to only express the necessary enzymes during exponential phase.
mutable notes: We recommend use of strain <bbpart>BBa_J45999</bbpart> as it does not produce indole. Indole is responsible for the foul smell of wt e.coli, and masks the wintergreen scent produced by J45800.
mutable provenance: This part is composed of <bbpart>BBa_J45320</bbpart> and <bbpart>BBa_J45180</bbpart>
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_J45320
component: BBa_J45180
sequence annotation: BBa_J45180
sequence annotation: BBa_J45320
sequence: https://synbiohub.org/public/igem/BBa_J45800_sequence/1

BBa_J45320
type: Component
persistent identity: component2291428
display id: component2291428
version: 1
top level: EWOBS
access: public
definition: SG

BBa_J45180
type: Component
persistent identity: component2291446
display id: component2291446
version: 1
top level: EWOBS
access: public
definition: https://synbiohub.org/public/igem/BBa_J45180/1

BBa_J45180
type: SequenceAnnotation
persistent identity: annotation2291446
display id: annotation2291446
version: 1
top level: EWOBS
location: https://synbiohub.org/public/igem/BBa_J45800/annotation2291446/range2291446/1
component: BBa_J45180

https://synbiohub.org/public/igem/BBa_J45800/annotation2291446/range2291446/1
type: Range
persistent identity: range2291446
display id: range2291446
version: 1
direction: direction/reverse
top level: EWOBS
start: 1964
end: 4168
orientation: inline

BBa_J45320
type: SequenceAnnotation
persistent identity: annotation2291428
display id: annotation2291428
version: 1
top level: EWOBS
location: https://synbiohub.org/public/igem/BBa_J45800/annotation2291428/range2291428/1
component: BBa_J45320

https://synbiohub.org/public/igem/BBa_J45800/annotation2291428/range2291428/1
type: Range
persistent identity: range2291428
display id: range2291428
version: 1
direction: direction/reverse
top level: EWOBS
start: 1
end: 1955
orientation: inline

BSMT1
type: ComponentDefinition
persistent identity: BBa J45119
display id: BBa_J45119
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Wintergreen odor enzyme (BMST1) generator
creator: Boyuan Zhu
created: 2006-08-09T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _84_
m user id: 0
owner id: 974
owning group id: 84
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This composite part include a ribosome binding site (B0032 different from J45099), the BSMT coding region, and a terminator. This part is attached to different promoters.
mutable notes: Used different RBS.
mutable provenance: Derived from the early part J45098 and sister to J45099.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_J45004
component: BBa_B0032
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_J45004
sequence annotation: BBa_B0010
sequence annotation: BBa_B0032
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_J45119_sequence/1

BBa_J45004
type: Component
persistent identity: component1894758
display id: component1894758
version: 1
top level: BSMT1
access: public
definition: BSMT1

BBa_B0032
type: Component
persistent identity: component1894753
display id: component1894753
version: 1
top level: BSMT1
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_B0012
type: Component
persistent identity: component1894761
display id: component1894761
version: 1
top level: BSMT1
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component1894759
display id: component1894759
version: 1
top level: BSMT1
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_J45004
type: SequenceAnnotation
persistent identity: annotation1894758
display id: annotation1894758
version: 1
top level: BSMT1
location: https://synbiohub.org/public/igem/BBa_J45119/annotation1894758/range1894758/1
component: BBa_J45004

https://synbiohub.org/public/igem/BBa_J45119/annotation1894758/range1894758/1
type: Range
persistent identity: range1894758
display id: range1894758
version: 1
direction: direction/reverse
top level: BSMT1
start: 20
end: 1093
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1894759
display id: annotation1894759
version: 1
top level: BSMT1
location: https://synbiohub.org/public/igem/BBa_J45119/annotation1894759/range1894759/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J45119/annotation1894759/range1894759/1
type: Range
persistent identity: range1894759
display id: range1894759
version: 1
direction: direction/forward
top level: BSMT1
start: 1102
end: 1181
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1894753
display id: annotation1894753
version: 1
top level: BSMT1
location: https://synbiohub.org/public/igem/BBa_J45119/annotation1894753/range1894753/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_J45119/annotation1894753/range1894753/1
type: Range
persistent identity: range1894753
display id: range1894753
version: 1
direction: direction/forward
top level: BSMT1
start: 1
end: 13
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1894761
display id: annotation1894761
version: 1
top level: BSMT1
location: https://synbiohub.org/public/igem/BBa_J45119/annotation1894761/range1894761/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J45119/annotation1894761/range1894761/1
type: Range
persistent identity: range1894761
display id: range1894761
version: 1
direction: direction/forward
top level: BSMT1
start: 1190
end: 1230
orientation: inline

BBa_J45991
type: ComponentDefinition
persistent identity: BBa J45991
display id: BBa_J45991
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: fd
creator: Boyuan Zhu
created: 2006-07-06T11:00:00Z
modified: 2015-08-31T04:08:50Z
discontinued: true
dominant: false
experience: experience/None
group u list: _84_
m user id: 0
owner id: 974
owning group id: 84
sample status: Discontinued
status: status/Deleted
bookmark: true
mutable description: dfa
mutable notes: dfa
mutable provenance: dfa
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000110
role: partType/Temporary
sequence: https://synbiohub.org/public/igem/BBa_J45991_sequence/1

p(osmY)
type: ComponentDefinition
persistent identity: BBa J45993
display id: BBa_J45993
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Minimal stationary phase osmY promoter
creator: Stephen Payne
created: 2006-07-10T11:00:00Z
modified: 2015-08-31T04:08:50Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _84_
m user id: 0
owner id: 642
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: osmY's promoter is active in stationary phase and under high osmotic pressure conditions.
mutable notes: Truncated Forward Primer: 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GGCT TAT GTT TTC GCT GAT ATC - 3' Total Length: 49 bp Annealing Length: 21 bp GC Content: 38.1% Melting Temperature: 49.5 degrees C hairpin deltaG: -2.66 kcal/mol self dimer deltaG: -99.97 kcal/mol Reverse Primer: 5'-GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TGT TAA ATA TAG ATC ACA ATT TTG- 3' Total Length: 54 bp Annealing Length: 25 bp GC Content: 20.0% Melting Temperature: 46.4 degrees C hairpin deltaG: -2.86 kcal/mol self dimer deltaG: -99.44 kcal/mol heterodimer deltaG with Conserved Forward Primer: -99.97 kcal/mol
mutable provenance: This part was PCRed out of E. coli's genome.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_J45993_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation1883647
display id: annotation1883647
version: 1
top level: p(osmY)
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J45993/annotation1883647/range1883647/1

https://synbiohub.org/public/igem/BBa_J45993/annotation1883647/range1883647/1
type: Range
persistent identity: range1883647
display id: range1883647
version: 1
direction: direction/forward
top level: p(osmY)
start: 46
end: 51
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1883648
display id: annotation1883648
version: 1
top level: p(osmY)
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J45993/annotation1883648/range1883648/1

https://synbiohub.org/public/igem/BBa_J45993/annotation1883648/range1883648/1
type: Range
persistent identity: range1883648
display id: range1883648
version: 1
direction: direction/forward
top level: p(osmY)
start: 22
end: 27
orientation: inline

YYC912
type: ComponentDefinition
persistent identity: BBa J45999
display id: BBa_J45999
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: E. coli strain YYC912; Odor-free chassis
creator: MIT iGEM 2006
created: 2006-10-30T12:00:00Z
modified: 2015-08-31T04:08:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _84_
m user id: 0
owner id: 126
owning group id: 70
sample status: No part sequence
status: status/Unavailable
bookmark: true
mutable description: Escherichia coli strain YYC912. Biosafety level 1 organism.
mutable notes: None.
mutable provenance: This strain was obtained from the E.coli Genetic Resources: CGSC, The Coli Genetic Stock Center http://cgsc.biology.yale.edu/. Strain YYC912.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Cell
role: SO:0000340

exp. GFP
type: ComponentDefinition
persistent identity: BBa J45996
display id: BBa_J45996
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Exponential phase dependent GFP generator
creator: Stephen Payne
created: 2006-10-30T12:00:00Z
modified: 2015-08-31T04:08:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _84_
m user id: 0
owner id: 642
owning group id: 84
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This composite part consists of <bbrick>J45992</bbrick> (a stationary phase promoter), <bbrick>Q04401</bbrick> (an inverter), and E0840 (a green fluorescent protein generator). This device expresses GFP in the exponential phase of cell growth.
mutable notes: This device was constructed to test whether the J45180 device would work.
mutable provenance: J45992 was PCRed out of the E. coli genome, while Q04401 and E0840 were developed by others in the Registry.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0010
component: BBa_B0012
component: BBa_E0040
component: BBa_J45992
component: BBa_B0030
component: BBa_Q04401
sequence annotation: BBa_B0010
sequence annotation: BBa_Q04401
sequence annotation: BBa_E0040
sequence annotation: BBa_B0030
sequence annotation: BBa_J45992
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_J45996_sequence/1

BBa_B0010
type: Component
persistent identity: component1909221
display id: component1909221
version: 1
top level: exp. GFP
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1909223
display id: component1909223
version: 1
top level: exp. GFP
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E0040
type: Component
persistent identity: component1909220
display id: component1909220
version: 1
top level: exp. GFP
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_J45992
type: Component
persistent identity: component1909215
display id: component1909215
version: 1
top level: exp. GFP
access: public
definition: p(osmY)

BBa_B0030
type: Component
persistent identity: component1909218
display id: component1909218
version: 1
top level: exp. GFP
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_Q04401
type: Component
persistent identity: component1909216
display id: component1909216
version: 1
top level: exp. GFP
access: public
definition: https://synbiohub.org/public/igem/BBa_Q04401/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1909221
display id: annotation1909221
version: 1
top level: exp. GFP
location: https://synbiohub.org/public/igem/BBa_J45996/annotation1909221/range1909221/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J45996/annotation1909221/range1909221/1
type: Range
persistent identity: range1909221
display id: range1909221
version: 1
direction: direction/forward
top level: exp. GFP
start: 1867
end: 1946
orientation: inline

BBa_Q04401
type: SequenceAnnotation
persistent identity: annotation1909216
display id: annotation1909216
version: 1
top level: exp. GFP
location: https://synbiohub.org/public/igem/BBa_J45996/annotation1909216/range1909216/1
component: BBa_Q04401

https://synbiohub.org/public/igem/BBa_J45996/annotation1909216/range1909216/1
type: Range
persistent identity: range1909216
display id: range1909216
version: 1
direction: direction/reverse
top level: exp. GFP
start: 208
end: 1109
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1909220
display id: annotation1909220
version: 1
top level: exp. GFP
location: https://synbiohub.org/public/igem/BBa_J45996/annotation1909220/range1909220/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_J45996/annotation1909220/range1909220/1
type: Range
persistent identity: range1909220
display id: range1909220
version: 1
direction: direction/reverse
top level: exp. GFP
start: 1139
end: 1858
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1909218
display id: annotation1909218
version: 1
top level: exp. GFP
location: https://synbiohub.org/public/igem/BBa_J45996/annotation1909218/range1909218/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_J45996/annotation1909218/range1909218/1
type: Range
persistent identity: range1909218
display id: range1909218
version: 1
direction: direction/forward
top level: exp. GFP
start: 1118
end: 1132
orientation: inline

BBa_J45992
type: SequenceAnnotation
persistent identity: annotation1909215
display id: annotation1909215
version: 1
top level: exp. GFP
location: https://synbiohub.org/public/igem/BBa_J45996/annotation1909215/range1909215/1
component: BBa_J45992

https://synbiohub.org/public/igem/BBa_J45996/annotation1909215/range1909215/1
type: Range
persistent identity: range1909215
display id: range1909215
version: 1
direction: direction/reverse
top level: exp. GFP
start: 1
end: 199
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1909223
display id: annotation1909223
version: 1
top level: exp. GFP
location: https://synbiohub.org/public/igem/BBa_J45996/annotation1909223/range1909223/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J45996/annotation1909223/range1909223/1
type: Range
persistent identity: range1909223
display id: range1909223
version: 1
direction: direction/forward
top level: exp. GFP
start: 1955
end: 1995
orientation: inline

Wintergree
type: ComponentDefinition
persistent identity: BBa J45181
display id: BBa_J45181
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Exponential phase dependent wintergreen odor generator
creator: Stephen Payne
created: 2007-02-10T12:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _84_
m user id: 0
owner id: 642
owning group id: 84
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part converts salicylic acid into methyl salicylate (a mint-smelling compound) in the exponential phase of cell growth. The part consists of a stationary phase promoter (J45992), connected to an inverter (Q04401) as well as the methyl salicylate protein generator (J45119). This part replaces the defective part J45180, which used the truncated version of the stationary phase promoter (J45993). This part did not function as it was designed due to an imbalance between the strength of the stationary phase promoter and the strength of the promoter in the inverter (R0040).
mutable notes: We needed the full osmY promoter strength to be relatively comparable to the R0040 promoter strength.
mutable provenance: Yes. It comes from many subparts.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_J45119
component: BBa_J45994
sequence annotation: BBa_J45994
sequence annotation: BBa_J45119
sequence: https://synbiohub.org/public/igem/BBa_J45181_sequence/1

BBa_J45119
type: Component
persistent identity: component2260490
display id: component2260490
version: 1
top level: Wintergree
access: public
definition: BSMT1

BBa_J45994
type: Component
persistent identity: component2260476
display id: component2260476
version: 1
top level: Wintergree
access: public
definition: Exponentia

BBa_J45994
type: SequenceAnnotation
persistent identity: annotation2260476
display id: annotation2260476
version: 1
top level: Wintergree
location: https://synbiohub.org/public/igem/BBa_J45181/annotation2260476/range2260476/1
component: BBa_J45994

https://synbiohub.org/public/igem/BBa_J45181/annotation2260476/range2260476/1
type: Range
persistent identity: range2260476
display id: range2260476
version: 1
direction: direction/reverse
top level: Wintergree
start: 1
end: 1109
orientation: inline

BBa_J45119
type: SequenceAnnotation
persistent identity: annotation2260490
display id: annotation2260490
version: 1
top level: Wintergree
location: https://synbiohub.org/public/igem/BBa_J45181/annotation2260490/range2260490/1
component: BBa_J45119

https://synbiohub.org/public/igem/BBa_J45181/annotation2260490/range2260490/1
type: Range
persistent identity: range2260490
display id: range2260490
version: 1
direction: direction/reverse
top level: Wintergree
start: 1118
end: 2347
orientation: inline

Banana
type: ComponentDefinition
persistent identity: BBa J45200
display id: BBa_J45200
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Banana odor generator
creator: Andr???? Green II
created: 2006-10-19T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _84_
m user id: 0
owner id: 977
owning group id: 84
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Protein generator converting PoPS to the protein ATF1. ATFI encodes the enzyme alcohol acetyltransferase I (AATase I), which produces acetate esters from acetyl CoA and alcohols.
mutable notes: This part is the ATF sequence BBa_45J006 with a single point mutation at base pair 1148 (T -> C). Despite the mutation, the enzyme is still functional (kinetic studies have not been done to determine realitive functionality).
mutable provenance: This part was cloned from S. cerevisiae.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0012
component: BBa_B0010
component: BBa_J45014
component: BBa_R0040
component: BBa_B0030
sequence annotation: BBa_B0030
sequence annotation: BBa_R0040
sequence annotation: BBa_B0010
sequence annotation: BBa_J45014
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_J45200_sequence/1

BBa_B0012
type: Component
persistent identity: component1960601
display id: component1960601
version: 1
top level: Banana
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component1960599
display id: component1960599
version: 1
top level: Banana
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_J45014
type: Component
persistent identity: component1960598
display id: component1960598
version: 1
top level: Banana
access: public
definition: ATF1

BBa_R0040
type: Component
persistent identity: component1960587
display id: component1960587
version: 1
top level: Banana
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0030
type: Component
persistent identity: component1960592
display id: component1960592
version: 1
top level: Banana
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1960592
display id: annotation1960592
version: 1
top level: Banana
location: https://synbiohub.org/public/igem/BBa_J45200/annotation1960592/range1960592/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_J45200/annotation1960592/range1960592/1
type: Range
persistent identity: range1960592
display id: range1960592
version: 1
direction: direction/forward
top level: Banana
start: 63
end: 77
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1960587
display id: annotation1960587
version: 1
top level: Banana
location: https://synbiohub.org/public/igem/BBa_J45200/annotation1960587/range1960587/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_J45200/annotation1960587/range1960587/1
type: Range
persistent identity: range1960587
display id: range1960587
version: 1
direction: direction/forward
top level: Banana
start: 1
end: 54
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1960599
display id: annotation1960599
version: 1
top level: Banana
location: https://synbiohub.org/public/igem/BBa_J45200/annotation1960599/range1960599/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J45200/annotation1960599/range1960599/1
type: Range
persistent identity: range1960599
display id: range1960599
version: 1
direction: direction/forward
top level: Banana
start: 1673
end: 1752
orientation: inline

BBa_J45014
type: SequenceAnnotation
persistent identity: annotation1960598
display id: annotation1960598
version: 1
top level: Banana
location: https://synbiohub.org/public/igem/BBa_J45200/annotation1960598/range1960598/1
component: BBa_J45014

https://synbiohub.org/public/igem/BBa_J45200/annotation1960598/range1960598/1
type: Range
persistent identity: range1960598
display id: range1960598
version: 1
direction: direction/reverse
top level: Banana
start: 84
end: 1664
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1960601
display id: annotation1960601
version: 1
top level: Banana
location: https://synbiohub.org/public/igem/BBa_J45200/annotation1960601/range1960601/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J45200/annotation1960601/range1960601/1
type: Range
persistent identity: range1960601
display id: range1960601
version: 1
direction: direction/forward
top level: Banana
start: 1761
end: 1801
orientation: inline

ATF1
type: ComponentDefinition
persistent identity: BBa J45014
display id: BBa_J45014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: alcohol acetyltransferase I; converts isoamyl alcohol to isoamyl acetate (banana odor)
creator: Andr?? Green II
created: 2006-07-30T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _84_
m user id: 0
owner id: 977
owning group id: 84
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This part is the ATF sequence (BBa_45J006) with a single point mutation at base pair 1176 (T -> C).
mutable notes: It has a single point mutation at base pair 1176 (T -> C).
mutable provenance: This part comes from BBa_45J006.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: ATF1
sequence annotation: a->g to eliminate EcoRI site
sequence annotation: t -> c
sequence annotation: double TAA stop codon
sequence: https://synbiohub.org/public/igem/BBa_J45014_sequence/1

ATF1
type: SequenceAnnotation
persistent identity: annotation1961300
display id: annotation1961300
version: 1
top level: ATF1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J45014/annotation1961300/range1961300/1

https://synbiohub.org/public/igem/BBa_J45014/annotation1961300/range1961300/1
type: Range
persistent identity: range1961300
display id: range1961300
version: 1
direction: direction/forward
top level: ATF1
start: 1
end: 1581
orientation: inline

a->g to eliminate EcoRI site
type: SequenceAnnotation
persistent identity: annotation1961305
display id: annotation1961305
version: 1
top level: ATF1
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_J45014/annotation1961305/range1961305/1

https://synbiohub.org/public/igem/BBa_J45014/annotation1961305/range1961305/1
type: Range
persistent identity: range1961305
display id: range1961305
version: 1
direction: direction/forward
top level: ATF1
start: 414
end: 414
orientation: inline

t -> c
type: SequenceAnnotation
persistent identity: annotation1961301
display id: annotation1961301
version: 1
top level: ATF1
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J45014/annotation1961301/range1961301/1

https://synbiohub.org/public/igem/BBa_J45014/annotation1961301/range1961301/1
type: Range
persistent identity: range1961301
display id: range1961301
version: 1
direction: direction/forward
top level: ATF1
start: 1148
end: 1148
orientation: inline

double TAA stop codon
type: SequenceAnnotation
persistent identity: annotation1961302
display id: annotation1961302
version: 1
top level: ATF1
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_J45014/annotation1961302/range1961302/1

https://synbiohub.org/public/igem/BBa_J45014/annotation1961302/range1961302/1
type: Range
persistent identity: range1961302
display id: range1961302
version: 1
direction: direction/forward
top level: ATF1
start: 1576
end: 1581
orientation: inline

SAMT
type: ComponentDefinition
persistent identity: BBa J45005
display id: BBa_J45005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SAM:salicylic acid carboxyl methyltransferase; converts salicylic acid to methyl salicylate (winterg
creator: Kate Broadbent
created: 2006-06-06T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: false
experience: experience/None
group u list: _84_
m user id: 0
owner id: 976
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: This is the SAMT enzyme from C. Breweri
mutable notes: we need an MTA or to sequence the coding region for this enzyme
mutable provenance: Genbank: AF133053, Eran Pichersky
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: SAMT
sequence annotation: double TAA stop codon
sequence annotation: start codon
sequence: https://synbiohub.org/public/igem/BBa_J45005_sequence/1

SAMT
type: SequenceAnnotation
persistent identity: annotation1880525
display id: annotation1880525
version: 1
top level: SAMT
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J45005/annotation1880525/range1880525/1

https://synbiohub.org/public/igem/BBa_J45005/annotation1880525/range1880525/1
type: Range
persistent identity: range1880525
display id: range1880525
version: 1
direction: direction/forward
top level: SAMT
start: 1
end: 1083
orientation: inline

double TAA stop codon
type: SequenceAnnotation
persistent identity: annotation1880522
display id: annotation1880522
version: 1
top level: SAMT
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_J45005/annotation1880522/range1880522/1

https://synbiohub.org/public/igem/BBa_J45005/annotation1880522/range1880522/1
type: Range
persistent identity: range1880522
display id: range1880522
version: 1
direction: direction/forward
top level: SAMT
start: 1078
end: 1083
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation1880479
display id: annotation1880479
version: 1
top level: SAMT
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J45005/annotation1880479/range1880479/1

https://synbiohub.org/public/igem/BBa_J45005/annotation1880479/range1880479/1
type: Range
persistent identity: range1880479
display id: range1880479
version: 1
direction: direction/forward
top level: SAMT
start: 1
end: 3
orientation: inline

SG
type: ComponentDefinition
persistent identity: BBa J45320
display id: BBa_J45320
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Salicylate generator
creator: Andr?? Green II
created: 2006-10-19T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _84_
m user id: 0
owner id: 977
owning group id: 84
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Protein generator converting PoPS to the prot BSMT. The BSMT enzyme converts benzoic acid/salicylic acid to methyl benzoate and methyl salicylate.
mutable notes: None
mutable provenance: This part is phBSMT1 from Petunia x hybrida (GenBank). See part design and discussion for more information.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_R0011
component: BBa_J45017
component: BBa_B0010
component: BBa_B0012
component: BBa_B0032
sequence annotation: BBa_R0011
sequence annotation: BBa_B0032
sequence annotation: BBa_J45017
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_J45320_sequence/1

BBa_R0011
type: Component
persistent identity: component1960945
display id: component1960945
version: 1
top level: SG
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_J45017
type: Component
persistent identity: component1960958
display id: component1960958
version: 1
top level: SG
access: public
definition: pchBA

BBa_B0010
type: Component
persistent identity: component1960959
display id: component1960959
version: 1
top level: SG
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1960961
display id: component1960961
version: 1
top level: SG
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0032
type: Component
persistent identity: component1960951
display id: component1960951
version: 1
top level: SG
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation1960945
display id: annotation1960945
version: 1
top level: SG
location: https://synbiohub.org/public/igem/BBa_J45320/annotation1960945/range1960945/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_J45320/annotation1960945/range1960945/1
type: Range
persistent identity: range1960945
display id: range1960945
version: 1
direction: direction/forward
top level: SG
start: 1
end: 54
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1960951
display id: annotation1960951
version: 1
top level: SG
location: https://synbiohub.org/public/igem/BBa_J45320/annotation1960951/range1960951/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_J45320/annotation1960951/range1960951/1
type: Range
persistent identity: range1960951
display id: range1960951
version: 1
direction: direction/forward
top level: SG
start: 64
end: 76
orientation: inline

BBa_J45017
type: SequenceAnnotation
persistent identity: annotation1960958
display id: annotation1960958
version: 1
top level: SG
location: https://synbiohub.org/public/igem/BBa_J45320/annotation1960958/range1960958/1
component: BBa_J45017

https://synbiohub.org/public/igem/BBa_J45320/annotation1960958/range1960958/1
type: Range
persistent identity: range1960958
display id: range1960958
version: 1
direction: direction/reverse
top level: SG
start: 83
end: 1818
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1960959
display id: annotation1960959
version: 1
top level: SG
location: https://synbiohub.org/public/igem/BBa_J45320/annotation1960959/range1960959/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J45320/annotation1960959/range1960959/1
type: Range
persistent identity: range1960959
display id: range1960959
version: 1
direction: direction/forward
top level: SG
start: 1827
end: 1906
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1960961
display id: annotation1960961
version: 1
top level: SG
location: https://synbiohub.org/public/igem/BBa_J45320/annotation1960961/range1960961/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J45320/annotation1960961/range1960961/1
type: Range
persistent identity: range1960961
display id: range1960961
version: 1
direction: direction/forward
top level: SG
start: 1915
end: 1955
orientation: inline

BAT2/THI3
type: ComponentDefinition
persistent identity: BBa J45398
display id: BBa_J45398
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BAT2 & THI3 enzyme generator
creator: Andr?? Green II
created: 2006-10-27T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _84_
m user id: 0
owner id: 977
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: This part is an intermediate to the protein generator BBa_45400.
mutable notes: coming
mutable provenance: coming
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_J45009
component: BBa_B0030
component: BBa_J45008
sequence annotation: BBa_B0030
sequence annotation: BBa_J45009
sequence annotation: BBa_J45008
sequence: https://synbiohub.org/public/igem/BBa_J45398_sequence/1

BBa_J45009
type: Component
persistent identity: component1960841
display id: component1960841
version: 1
top level: BAT2/THI3
access: public
definition: THI3

BBa_B0030
type: Component
persistent identity: component1960834
display id: component1960834
version: 1
top level: BAT2/THI3
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_B0030
type: Component
persistent identity: component1960827
display id: component1960827
version: 1
top level: BAT2/THI3
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_J45008
type: Component
persistent identity: component1960832
display id: component1960832
version: 1
top level: BAT2/THI3
access: public
definition: BAT2

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1960834
display id: annotation1960834
version: 1
top level: BAT2/THI3
location: https://synbiohub.org/public/igem/BBa_J45398/annotation1960834/range1960834/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_J45398/annotation1960834/range1960834/1
type: Range
persistent identity: range1960834
display id: range1960834
version: 1
direction: direction/forward
top level: BAT2/THI3
start: 1164
end: 1178
orientation: inline

BBa_J45009
type: SequenceAnnotation
persistent identity: annotation1960841
display id: annotation1960841
version: 1
top level: BAT2/THI3
location: https://synbiohub.org/public/igem/BBa_J45398/annotation1960841/range1960841/1
component: BBa_J45009

https://synbiohub.org/public/igem/BBa_J45398/annotation1960841/range1960841/1
type: Range
persistent identity: range1960841
display id: range1960841
version: 1
direction: direction/reverse
top level: BAT2/THI3
start: 1185
end: 3014
orientation: inline

BBa_J45008
type: SequenceAnnotation
persistent identity: annotation1960832
display id: annotation1960832
version: 1
top level: BAT2/THI3
location: https://synbiohub.org/public/igem/BBa_J45398/annotation1960832/range1960832/1
component: BBa_J45008

https://synbiohub.org/public/igem/BBa_J45398/annotation1960832/range1960832/1
type: Range
persistent identity: range1960832
display id: range1960832
version: 1
direction: direction/reverse
top level: BAT2/THI3
start: 22
end: 1155
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1960827
display id: annotation1960827
version: 1
top level: BAT2/THI3
location: https://synbiohub.org/public/igem/BBa_J45398/annotation1960827/range1960827/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_J45398/annotation1960827/range1960827/1
type: Range
persistent identity: range1960827
display id: range1960827
version: 1
direction: direction/forward
top level: BAT2/THI3
start: 1
end: 15
orientation: inline

BOBS
type: ComponentDefinition
persistent identity: BBa J45900
display id: BBa_J45900
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Banana odor biosynthetic system
creator: Stephen Payne
created: 2008-02-04T12:00:00Z
modified: 2015-08-31T04:08:50Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _84_
m user id: 0
owner id: 642
owning group id: 84
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part does not work.
mutable notes: This part has EcoRI and SpeI sites in it. Use caution when conducting standard assembly with the part.
mutable provenance: This part was made via standard assembly of J45200 and J45400.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_J45008
component: BBa_J45009
component: BBa_B0012
component: BBa_B0030
component: BBa_R0011
component: BBa_R0040
component: BBa_B0010
component: BBa_J45014
sequence annotation: BBa_B0012
sequence annotation: BBa_J45009
sequence annotation: BBa_J45008
sequence annotation: BBa_B0010
sequence annotation: BBa_J45014
sequence annotation: BBa_R0011
sequence annotation: BBa_B0030
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_J45900_sequence/1

BBa_J45008
type: Component
persistent identity: component1960853
display id: component1960853
version: 1
top level: BOBS
access: public
definition: BAT2

BBa_J45009
type: Component
persistent identity: component1960862
display id: component1960862
version: 1
top level: BOBS
access: public
definition: THI3

BBa_B0012
type: Component
persistent identity: component1960878
display id: component1960878
version: 1
top level: BOBS
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0030
type: Component
persistent identity: component1960848
display id: component1960848
version: 1
top level: BOBS
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_R0011
type: Component
persistent identity: component1960842
display id: component1960842
version: 1
top level: BOBS
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_B0030
type: Component
persistent identity: component1960855
display id: component1960855
version: 1
top level: BOBS
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_R0040
type: Component
persistent identity: component1960864
display id: component1960864
version: 1
top level: BOBS
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0010
type: Component
persistent identity: component1960876
display id: component1960876
version: 1
top level: BOBS
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_J45014
type: Component
persistent identity: component1960875
display id: component1960875
version: 1
top level: BOBS
access: public
definition: ATF1

BBa_B0030
type: Component
persistent identity: component1960869
display id: component1960869
version: 1
top level: BOBS
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1960878
display id: annotation1960878
version: 1
top level: BOBS
location: https://synbiohub.org/public/igem/BBa_J45900/annotation1960878/range1960878/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J45900/annotation1960878/range1960878/1
type: Range
persistent identity: range1960878
display id: range1960878
version: 1
direction: direction/forward
top level: BOBS
start: 4846
end: 4886
orientation: inline

BBa_J45009
type: SequenceAnnotation
persistent identity: annotation1960862
display id: annotation1960862
version: 1
top level: BOBS
location: https://synbiohub.org/public/igem/BBa_J45900/annotation1960862/range1960862/1
component: BBa_J45009

https://synbiohub.org/public/igem/BBa_J45900/annotation1960862/range1960862/1
type: Range
persistent identity: range1960862
display id: range1960862
version: 1
direction: direction/reverse
top level: BOBS
start: 1248
end: 3077
orientation: inline

BBa_J45008
type: SequenceAnnotation
persistent identity: annotation1960853
display id: annotation1960853
version: 1
top level: BOBS
location: https://synbiohub.org/public/igem/BBa_J45900/annotation1960853/range1960853/1
component: BBa_J45008

https://synbiohub.org/public/igem/BBa_J45900/annotation1960853/range1960853/1
type: Range
persistent identity: range1960853
display id: range1960853
version: 1
direction: direction/reverse
top level: BOBS
start: 85
end: 1218
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1960876
display id: annotation1960876
version: 1
top level: BOBS
location: https://synbiohub.org/public/igem/BBa_J45900/annotation1960876/range1960876/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J45900/annotation1960876/range1960876/1
type: Range
persistent identity: range1960876
display id: range1960876
version: 1
direction: direction/forward
top level: BOBS
start: 4758
end: 4837
orientation: inline

BBa_J45014
type: SequenceAnnotation
persistent identity: annotation1960875
display id: annotation1960875
version: 1
top level: BOBS
location: https://synbiohub.org/public/igem/BBa_J45900/annotation1960875/range1960875/1
component: BBa_J45014

https://synbiohub.org/public/igem/BBa_J45900/annotation1960875/range1960875/1
type: Range
persistent identity: range1960875
display id: range1960875
version: 1
direction: direction/reverse
top level: BOBS
start: 3169
end: 4749
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation1960842
display id: annotation1960842
version: 1
top level: BOBS
location: https://synbiohub.org/public/igem/BBa_J45900/annotation1960842/range1960842/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_J45900/annotation1960842/range1960842/1
type: Range
persistent identity: range1960842
display id: range1960842
version: 1
direction: direction/forward
top level: BOBS
start: 1
end: 54
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1960869
display id: annotation1960869
version: 1
top level: BOBS
location: https://synbiohub.org/public/igem/BBa_J45900/annotation1960869/range1960869/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_J45900/annotation1960869/range1960869/1
type: Range
persistent identity: range1960869
display id: range1960869
version: 1
direction: direction/forward
top level: BOBS
start: 3148
end: 3162
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1960848
display id: annotation1960848
version: 1
top level: BOBS
location: https://synbiohub.org/public/igem/BBa_J45900/annotation1960848/range1960848/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_J45900/annotation1960848/range1960848/1
type: Range
persistent identity: range1960848
display id: range1960848
version: 1
direction: direction/forward
top level: BOBS
start: 64
end: 78
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1960864
display id: annotation1960864
version: 1
top level: BOBS
location: https://synbiohub.org/public/igem/BBa_J45900/annotation1960864/range1960864/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_J45900/annotation1960864/range1960864/1
type: Range
persistent identity: range1960864
display id: range1960864
version: 1
direction: direction/forward
top level: BOBS
start: 3086
end: 3139
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1960855
display id: annotation1960855
version: 1
top level: BOBS
location: https://synbiohub.org/public/igem/BBa_J45900/annotation1960855/range1960855/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_J45900/annotation1960855/range1960855/1
type: Range
persistent identity: range1960855
display id: range1960855
version: 1
direction: direction/forward
top level: BOBS
start: 1227
end: 1241
orientation: inline

BAT2
type: ComponentDefinition
persistent identity: BBa J45008
display id: BBa_J45008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: branched-chain amino acid transaminase (BAT2); converts leucine to alpha-ketoisocaproate
creator: Veena Venkatachalam
created: 2006-06-06T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _84_
m user id: 0
owner id: 642
owning group id: 84
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: fd;lsfas
mutable notes: dakfdas;
mutable provenance: dkfkas
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BAT2
sequence annotation: double TAA stop codon
sequence annotation: start codon
sequence annotation: a->g to eliminate SpeI
sequence: https://synbiohub.org/public/igem/BBa_J45008_sequence/1

BAT2
type: SequenceAnnotation
persistent identity: annotation1961299
display id: annotation1961299
version: 1
top level: BAT2
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J45008/annotation1961299/range1961299/1

https://synbiohub.org/public/igem/BBa_J45008/annotation1961299/range1961299/1
type: Range
persistent identity: range1961299
display id: range1961299
version: 1
direction: direction/forward
top level: BAT2
start: 1
end: 1134
orientation: inline

double TAA stop codon
type: SequenceAnnotation
persistent identity: annotation1883625
display id: annotation1883625
version: 1
top level: BAT2
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_J45008/annotation1883625/range1883625/1

https://synbiohub.org/public/igem/BBa_J45008/annotation1883625/range1883625/1
type: Range
persistent identity: range1883625
display id: range1883625
version: 1
direction: direction/forward
top level: BAT2
start: 1129
end: 1134
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation1883624
display id: annotation1883624
version: 1
top level: BAT2
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J45008/annotation1883624/range1883624/1

https://synbiohub.org/public/igem/BBa_J45008/annotation1883624/range1883624/1
type: Range
persistent identity: range1883624
display id: range1883624
version: 1
direction: direction/forward
top level: BAT2
start: 1
end: 3
orientation: inline

a->g to eliminate SpeI
type: SequenceAnnotation
persistent identity: annotation1894553
display id: annotation1894553
version: 1
top level: BAT2
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_J45008/annotation1894553/range1894553/1

https://synbiohub.org/public/igem/BBa_J45008/annotation1894553/range1894553/1
type: Range
persistent identity: range1894553
display id: range1894553
version: 1
direction: direction/forward
top level: BAT2
start: 771
end: 771
orientation: inline

ATF1 gener
type: ComponentDefinition
persistent identity: BBa J45199
display id: BBa_J45199
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Banana odor enzyme (ATF1) generator
creator: Andr?? Green II
created: 2006-10-27T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _84_
m user id: 0
owner id: 977
owning group id: 84
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This part is an intermediate to the protein generator BBa_45200.
mutable notes: coming
mutable provenance: coming
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0010
component: BBa_B0030
component: BBa_J45014
component: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_B0030
sequence annotation: BBa_J45014
sequence: https://synbiohub.org/public/igem/BBa_J45199_sequence/1

BBa_B0010
type: Component
persistent identity: component1908016
display id: component1908016
version: 1
top level: ATF1 gener
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0030
type: Component
persistent identity: component1908009
display id: component1908009
version: 1
top level: ATF1 gener
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_J45014
type: Component
persistent identity: component1908015
display id: component1908015
version: 1
top level: ATF1 gener
access: public
definition: ATF1

BBa_B0012
type: Component
persistent identity: component1908018
display id: component1908018
version: 1
top level: ATF1 gener
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1908016
display id: annotation1908016
version: 1
top level: ATF1 gener
location: https://synbiohub.org/public/igem/BBa_J45199/annotation1908016/range1908016/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J45199/annotation1908016/range1908016/1
type: Range
persistent identity: range1908016
display id: range1908016
version: 1
direction: direction/forward
top level: ATF1 gener
start: 1611
end: 1690
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1908018
display id: annotation1908018
version: 1
top level: ATF1 gener
location: https://synbiohub.org/public/igem/BBa_J45199/annotation1908018/range1908018/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J45199/annotation1908018/range1908018/1
type: Range
persistent identity: range1908018
display id: range1908018
version: 1
direction: direction/forward
top level: ATF1 gener
start: 1699
end: 1739
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1908009
display id: annotation1908009
version: 1
top level: ATF1 gener
location: https://synbiohub.org/public/igem/BBa_J45199/annotation1908009/range1908009/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_J45199/annotation1908009/range1908009/1
type: Range
persistent identity: range1908009
display id: range1908009
version: 1
direction: direction/forward
top level: ATF1 gener
start: 1
end: 15
orientation: inline

BBa_J45014
type: SequenceAnnotation
persistent identity: annotation1908015
display id: annotation1908015
version: 1
top level: ATF1 gener
location: https://synbiohub.org/public/igem/BBa_J45199/annotation1908015/range1908015/1
component: BBa_J45014

https://synbiohub.org/public/igem/BBa_J45199/annotation1908015/range1908015/1
type: Range
persistent identity: range1908015
display id: range1908015
version: 1
direction: direction/reverse
top level: ATF1 gener
start: 22
end: 1602
orientation: inline

SBOBS
type: ComponentDefinition
persistent identity: BBa J45600
display id: BBa_J45600
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Stationary phase banana odor biosynthetic system
creator: MIT iGEM 2006
created: 2006-10-30T12:00:00Z
modified: 2015-08-31T04:08:50Z
discontinued: false
dominant: false
experience: experience/None
group u list: _84_
m user id: 0
owner id: 2
owning group id: 84
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This system designed by the MIT 2006 iGEM team generates mint smell by biosynthesizing salicylic acid from chassis-produced metabolites (chorismate) and then converts salicylic acid into methyl salicylate (wintergreen scent).
mutable notes: We recommend use of strain <bbpart>BBa_J45999</bbpart> as it does not produce indole. Indole is responsible for the foul smell of wt e.coli, and masks the wintergreen scent produced by J45600.
mutable provenance: This system is composed of devices <bbpart>J45120</bbpart> and <bbpart>J45320</bbpart>.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_B0012
component: BBa_B0010
component: BBa_J45008
component: BBa_R0011
component: BBa_B0030
component: BBa_J45014
component: BBa_J45009
component: BBa_J45992
sequence annotation: BBa_B0012
sequence annotation: BBa_J45009
sequence annotation: BBa_R0011
sequence annotation: BBa_B0010
sequence annotation: BBa_J45014
sequence annotation: BBa_J45992
sequence annotation: BBa_J45008
sequence annotation: BBa_B0030
sequence: https://synbiohub.org/public/igem/BBa_J45600_sequence/1

BBa_B0012
type: Component
persistent identity: component1961073
display id: component1961073
version: 1
top level: SBOBS
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component1961071
display id: component1961071
version: 1
top level: SBOBS
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_J45008
type: Component
persistent identity: component1961088
display id: component1961088
version: 1
top level: SBOBS
access: public
definition: BAT2

BBa_R0011
type: Component
persistent identity: component1961077
display id: component1961077
version: 1
top level: SBOBS
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_B0030
type: Component
persistent identity: component1961064
display id: component1961064
version: 1
top level: SBOBS
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_J45014
type: Component
persistent identity: component1961070
display id: component1961070
version: 1
top level: SBOBS
access: public
definition: ATF1

BBa_B0030
type: Component
persistent identity: component1961083
display id: component1961083
version: 1
top level: SBOBS
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_J45009
type: Component
persistent identity: component1961097
display id: component1961097
version: 1
top level: SBOBS
access: public
definition: THI3

BBa_J45992
type: Component
persistent identity: component1961062
display id: component1961062
version: 1
top level: SBOBS
access: public
definition: p(osmY)

BBa_B0030
type: Component
persistent identity: component1961090
display id: component1961090
version: 1
top level: SBOBS
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1961073
display id: annotation1961073
version: 1
top level: SBOBS
location: https://synbiohub.org/public/igem/BBa_J45600/annotation1961073/range1961073/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J45600/annotation1961073/range1961073/1
type: Range
persistent identity: range1961073
display id: range1961073
version: 1
direction: direction/forward
top level: SBOBS
start: 1906
end: 1946
orientation: inline

BBa_J45009
type: SequenceAnnotation
persistent identity: annotation1961097
display id: annotation1961097
version: 1
top level: SBOBS
location: https://synbiohub.org/public/igem/BBa_J45600/annotation1961097/range1961097/1
component: BBa_J45009

https://synbiohub.org/public/igem/BBa_J45600/annotation1961097/range1961097/1
type: Range
persistent identity: range1961097
display id: range1961097
version: 1
direction: direction/reverse
top level: SBOBS
start: 3202
end: 5031
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation1961077
display id: annotation1961077
version: 1
top level: SBOBS
location: https://synbiohub.org/public/igem/BBa_J45600/annotation1961077/range1961077/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_J45600/annotation1961077/range1961077/1
type: Range
persistent identity: range1961077
display id: range1961077
version: 1
direction: direction/forward
top level: SBOBS
start: 1955
end: 2008
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1961071
display id: annotation1961071
version: 1
top level: SBOBS
location: https://synbiohub.org/public/igem/BBa_J45600/annotation1961071/range1961071/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J45600/annotation1961071/range1961071/1
type: Range
persistent identity: range1961071
display id: range1961071
version: 1
direction: direction/forward
top level: SBOBS
start: 1818
end: 1897
orientation: inline

BBa_J45014
type: SequenceAnnotation
persistent identity: annotation1961070
display id: annotation1961070
version: 1
top level: SBOBS
location: https://synbiohub.org/public/igem/BBa_J45600/annotation1961070/range1961070/1
component: BBa_J45014

https://synbiohub.org/public/igem/BBa_J45600/annotation1961070/range1961070/1
type: Range
persistent identity: range1961070
display id: range1961070
version: 1
direction: direction/reverse
top level: SBOBS
start: 229
end: 1809
orientation: inline

BBa_J45992
type: SequenceAnnotation
persistent identity: annotation1961062
display id: annotation1961062
version: 1
top level: SBOBS
location: https://synbiohub.org/public/igem/BBa_J45600/annotation1961062/range1961062/1
component: BBa_J45992

https://synbiohub.org/public/igem/BBa_J45600/annotation1961062/range1961062/1
type: Range
persistent identity: range1961062
display id: range1961062
version: 1
direction: direction/reverse
top level: SBOBS
start: 1
end: 199
orientation: inline

BBa_J45008
type: SequenceAnnotation
persistent identity: annotation1961088
display id: annotation1961088
version: 1
top level: SBOBS
location: https://synbiohub.org/public/igem/BBa_J45600/annotation1961088/range1961088/1
component: BBa_J45008

https://synbiohub.org/public/igem/BBa_J45600/annotation1961088/range1961088/1
type: Range
persistent identity: range1961088
display id: range1961088
version: 1
direction: direction/reverse
top level: SBOBS
start: 2039
end: 3172
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1961090
display id: annotation1961090
version: 1
top level: SBOBS
location: https://synbiohub.org/public/igem/BBa_J45600/annotation1961090/range1961090/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_J45600/annotation1961090/range1961090/1
type: Range
persistent identity: range1961090
display id: range1961090
version: 1
direction: direction/forward
top level: SBOBS
start: 3181
end: 3195
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1961083
display id: annotation1961083
version: 1
top level: SBOBS
location: https://synbiohub.org/public/igem/BBa_J45600/annotation1961083/range1961083/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_J45600/annotation1961083/range1961083/1
type: Range
persistent identity: range1961083
display id: range1961083
version: 1
direction: direction/forward
top level: SBOBS
start: 2018
end: 2032
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1961064
display id: annotation1961064
version: 1
top level: SBOBS
location: https://synbiohub.org/public/igem/BBa_J45600/annotation1961064/range1961064/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_J45600/annotation1961064/range1961064/1
type: Range
persistent identity: range1961064
display id: range1961064
version: 1
direction: direction/forward
top level: SBOBS
start: 208
end: 222
orientation: inline

p(osmY)
type: ComponentDefinition
persistent identity: BBa J45992
display id: BBa_J45992
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Full-length stationary phase osmY promoter
creator: Stephen Payne
created: 2006-07-10T11:00:00Z
modified: 2015-08-31T04:08:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _84_
m user id: 0
owner id: 642
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: osmY's promoter is active in stationary phase and under high osmotic pressure conditions.
mutable notes: Truncated Forward Primer: 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GGCT TAT GTT TTC GCT GAT ATC - 3' Total Length: 49 bp Annealing Length: 21 bp GC Content: 38.1% Melting Temperature: 49.5 degrees C hairpin deltaG: -2.66 kcal/mol self dimer deltaG: -99.97 kcal/mol Full Forward Primer: 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GCTG GCA CAG GAA CGT TAT C - 3' Total Length: 47 bp Annealing Length: 19 bp GC Content: 52.6% Melting Temperature: 53.4 degrees C hairpin deltaG: -3.99 kcal/mol self dimer deltaG: -98.3 kcal/mol Reverse Primer: 5'-GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TGT TAA ATA TAG ATC ACA ATT TTG- 3' Total Length: 54 bp Annealing Length: 25 bp GC Content: 20.0% Melting Temperature: 46.4 degrees C hairpin deltaG: -2.86 kcal/mol self dimer deltaG: -99.44 kcal/mol heterodimer deltaG with Forward Primer: -99.44 kcal/mol heterodimer deltaG with Conserved Forward Primer: -99.97 kcal/mol
mutable provenance: This part was PCRed out of E. coli's genome.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_J45992_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation1883646
display id: annotation1883646
version: 1
top level: p(osmY)
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J45992/annotation1883646/range1883646/1

https://synbiohub.org/public/igem/BBa_J45992/annotation1883646/range1883646/1
type: Range
persistent identity: range1883646
display id: range1883646
version: 1
direction: direction/forward
top level: p(osmY)
start: 164
end: 169
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1883645
display id: annotation1883645
version: 1
top level: p(osmY)
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J45992/annotation1883645/range1883645/1

https://synbiohub.org/public/igem/BBa_J45992/annotation1883645/range1883645/1
type: Range
persistent identity: range1883645
display id: range1883645
version: 1
direction: direction/forward
top level: p(osmY)
start: 188
end: 193
orientation: inline

pchBA
type: ComponentDefinition
persistent identity: BBa J45017
display id: BBa_J45017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: isochorismate pyruvate-lyase and isochorismate synthase (pchBA); converts chorismate to salicylate
creator: MIT IGEM 2006
created: 2006-08-20T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _84_
m user id: 0
owner id: 1147
owning group id: 84
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: pchBA codes for proteins used to generate salicylic acid. It doesn't make a fusion protein, even though the coding regions overlap; the RBS for pchA is located at the end of the coding region of pchB.
mutable notes: n/a
mutable provenance: pchBA is found in pseudomonas. The plasmid was kindly sent to the MIT iGEM team by Dr. Cornelia Riemmann from the Universite de Lausanne.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: pchA start codon
sequence annotation: pchB stop codon
sequence annotation: g -> a to eliminate PstI site
sequence annotation: PchA
sequence annotation: pchB start codon
sequence annotation: pchA double TAA stop codon
sequence annotation: PchB
sequence: https://synbiohub.org/public/igem/BBa_J45017_sequence/1

pchA start codon
type: SequenceAnnotation
persistent identity: annotation1897454
display id: annotation1897454
version: 1
top level: pchBA
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J45017/annotation1897454/range1897454/1

https://synbiohub.org/public/igem/BBa_J45017/annotation1897454/range1897454/1
type: Range
persistent identity: range1897454
display id: range1897454
version: 1
direction: direction/forward
top level: pchBA
start: 303
end: 305
orientation: inline

pchB stop codon
type: SequenceAnnotation
persistent identity: annotation1897456
display id: annotation1897456
version: 1
top level: pchBA
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_J45017/annotation1897456/range1897456/1

https://synbiohub.org/public/igem/BBa_J45017/annotation1897456/range1897456/1
type: Range
persistent identity: range1897456
display id: range1897456
version: 1
direction: direction/forward
top level: pchBA
start: 304
end: 306
orientation: inline

g -> a to eliminate PstI site
type: SequenceAnnotation
persistent identity: annotation1897453
display id: annotation1897453
version: 1
top level: pchBA
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_J45017/annotation1897453/range1897453/1

https://synbiohub.org/public/igem/BBa_J45017/annotation1897453/range1897453/1
type: Range
persistent identity: range1897453
display id: range1897453
version: 1
direction: direction/forward
top level: pchBA
start: 440
end: 440
orientation: inline

PchA
type: SequenceAnnotation
persistent identity: annotation1961297
display id: annotation1961297
version: 1
top level: pchBA
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J45017/annotation1961297/range1961297/1

https://synbiohub.org/public/igem/BBa_J45017/annotation1961297/range1961297/1
type: Range
persistent identity: range1961297
display id: range1961297
version: 1
direction: direction/forward
top level: pchBA
start: 303
end: 1736
orientation: inline

pchB start codon
type: SequenceAnnotation
persistent identity: annotation1897455
display id: annotation1897455
version: 1
top level: pchBA
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J45017/annotation1897455/range1897455/1

https://synbiohub.org/public/igem/BBa_J45017/annotation1897455/range1897455/1
type: Range
persistent identity: range1897455
display id: range1897455
version: 1
direction: direction/forward
top level: pchBA
start: 1
end: 3
orientation: inline

pchA double TAA stop codon
type: SequenceAnnotation
persistent identity: annotation1897457
display id: annotation1897457
version: 1
top level: pchBA
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_J45017/annotation1897457/range1897457/1

https://synbiohub.org/public/igem/BBa_J45017/annotation1897457/range1897457/1
type: Range
persistent identity: range1897457
display id: range1897457
version: 1
direction: direction/forward
top level: pchBA
start: 1731
end: 1736
orientation: inline

PchB
type: SequenceAnnotation
persistent identity: annotation1961296
display id: annotation1961296
version: 1
top level: pchBA
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J45017/annotation1961296/range1961296/1

https://synbiohub.org/public/igem/BBa_J45017/annotation1961296/range1961296/1
type: Range
persistent identity: range1961296
display id: range1961296
version: 1
direction: direction/forward
top level: pchBA
start: 1
end: 306
orientation: inline

WOBS
type: ComponentDefinition
persistent identity: BBa J45700
display id: BBa_J45700
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Wintergreen odor biosynthetic system
creator: MIT iGEM 2006
created: 2006-10-30T12:00:00Z
modified: 2015-08-31T04:08:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _84_
m user id: 0
owner id: 2
owning group id: 84
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This system designed by the MIT 2006 iGEM team and generates mint smell by biosynthesizing salicylic acid from chassis-produced metabolites (chorismate) and then converts salicylic acid into methyl salicylate (wintergreen scent).
mutable notes: We recommend use of strain <bbpart>BBa_J45999</bbpart> as it does not produce indole. Indole is responsible for the foul smell of wt e.coli, and masks the wintergreen scent produced by J45600.
mutable provenance: This system is composed of devices <bbpart>J45120</bbpart> and <bbpart>J45320</bbpart>.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_R0011
component: BBa_B0010
component: BBa_B0032
component: BBa_J45004
component: BBa_J45017
component: BBa_B0012
component: BBa_R0040
sequence annotation: BBa_B0012
sequence annotation: BBa_R0040
sequence annotation: BBa_J45004
sequence annotation: BBa_B0032
sequence annotation: BBa_B0010
sequence annotation: BBa_R0011
sequence annotation: BBa_J45017
sequence: https://synbiohub.org/public/igem/BBa_J45700_sequence/1

BBa_R0011
type: Component
persistent identity: component1960983
display id: component1960983
version: 1
top level: WOBS
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_B0010
type: Component
persistent identity: component1960997
display id: component1960997
version: 1
top level: WOBS
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0032
type: Component
persistent identity: component1960971
display id: component1960971
version: 1
top level: WOBS
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_J45004
type: Component
persistent identity: component1960976
display id: component1960976
version: 1
top level: WOBS
access: public
definition: BSMT1

BBa_J45017
type: Component
persistent identity: component1960996
display id: component1960996
version: 1
top level: WOBS
access: public
definition: pchBA

BBa_B0012
type: Component
persistent identity: component1960999
display id: component1960999
version: 1
top level: WOBS
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0032
type: Component
persistent identity: component1960989
display id: component1960989
version: 1
top level: WOBS
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_B0010
type: Component
persistent identity: component1960977
display id: component1960977
version: 1
top level: WOBS
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1960979
display id: component1960979
version: 1
top level: WOBS
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0040
type: Component
persistent identity: component1960966
display id: component1960966
version: 1
top level: WOBS
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1960999
display id: annotation1960999
version: 1
top level: WOBS
location: https://synbiohub.org/public/igem/BBa_J45700/annotation1960999/range1960999/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J45700/annotation1960999/range1960999/1
type: Range
persistent identity: range1960999
display id: range1960999
version: 1
direction: direction/forward
top level: WOBS
start: 3215
end: 3255
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1960966
display id: annotation1960966
version: 1
top level: WOBS
location: https://synbiohub.org/public/igem/BBa_J45700/annotation1960966/range1960966/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_J45700/annotation1960966/range1960966/1
type: Range
persistent identity: range1960966
display id: range1960966
version: 1
direction: direction/forward
top level: WOBS
start: 1
end: 54
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1960979
display id: annotation1960979
version: 1
top level: WOBS
location: https://synbiohub.org/public/igem/BBa_J45700/annotation1960979/range1960979/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J45700/annotation1960979/range1960979/1
type: Range
persistent identity: range1960979
display id: range1960979
version: 1
direction: direction/forward
top level: WOBS
start: 1252
end: 1292
orientation: inline

BBa_J45004
type: SequenceAnnotation
persistent identity: annotation1960976
display id: annotation1960976
version: 1
top level: WOBS
location: https://synbiohub.org/public/igem/BBa_J45700/annotation1960976/range1960976/1
component: BBa_J45004

https://synbiohub.org/public/igem/BBa_J45700/annotation1960976/range1960976/1
type: Range
persistent identity: range1960976
display id: range1960976
version: 1
direction: direction/reverse
top level: WOBS
start: 82
end: 1155
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1960989
display id: annotation1960989
version: 1
top level: WOBS
location: https://synbiohub.org/public/igem/BBa_J45700/annotation1960989/range1960989/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_J45700/annotation1960989/range1960989/1
type: Range
persistent identity: range1960989
display id: range1960989
version: 1
direction: direction/forward
top level: WOBS
start: 1364
end: 1376
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1960997
display id: annotation1960997
version: 1
top level: WOBS
location: https://synbiohub.org/public/igem/BBa_J45700/annotation1960997/range1960997/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J45700/annotation1960997/range1960997/1
type: Range
persistent identity: range1960997
display id: range1960997
version: 1
direction: direction/forward
top level: WOBS
start: 3127
end: 3206
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1960977
display id: annotation1960977
version: 1
top level: WOBS
location: https://synbiohub.org/public/igem/BBa_J45700/annotation1960977/range1960977/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J45700/annotation1960977/range1960977/1
type: Range
persistent identity: range1960977
display id: range1960977
version: 1
direction: direction/forward
top level: WOBS
start: 1164
end: 1243
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation1960983
display id: annotation1960983
version: 1
top level: WOBS
location: https://synbiohub.org/public/igem/BBa_J45700/annotation1960983/range1960983/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_J45700/annotation1960983/range1960983/1
type: Range
persistent identity: range1960983
display id: range1960983
version: 1
direction: direction/forward
top level: WOBS
start: 1301
end: 1354
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1960971
display id: annotation1960971
version: 1
top level: WOBS
location: https://synbiohub.org/public/igem/BBa_J45700/annotation1960971/range1960971/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_J45700/annotation1960971/range1960971/1
type: Range
persistent identity: range1960971
display id: range1960971
version: 1
direction: direction/forward
top level: WOBS
start: 63
end: 75
orientation: inline

BBa_J45017
type: SequenceAnnotation
persistent identity: annotation1960996
display id: annotation1960996
version: 1
top level: WOBS
location: https://synbiohub.org/public/igem/BBa_J45700/annotation1960996/range1960996/1
component: BBa_J45017

https://synbiohub.org/public/igem/BBa_J45700/annotation1960996/range1960996/1
type: Range
persistent identity: range1960996
display id: range1960996
version: 1
direction: direction/reverse
top level: WOBS
start: 1383
end: 3118
orientation: inline