//rnap/prokaryote/subtilis/sigmab
type: Collection
persistent identity: rnap prokaryote subtilis sigmab
display id: rnap_prokaryote_subtilis_sigmab
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: Pctc
member: P43
member: PgsiB

//rnap/prokaryote/subtilis
type: Collection
persistent identity: rnap prokaryote subtilis
display id: rnap_prokaryote_subtilis
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: //rnap/prokaryote/subtilis/sigmaa
member: //rnap/prokaryote/subtilis/sigmab

//rnap/prokaryote/subtilis/sigmaa
type: Collection
persistent identity: rnap prokaryote subtilis sigmaa
display id: rnap_prokaryote_subtilis_sigmaa
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: BBa_K823002
member: BBa_K823001
member: BBa_K823003
member: Pveg
member: P43
member: BBa_K1074013
member: BBa_K823000
member: BBa_K090504
member: Pxyl
member: BBa_K090501
member: Ph-s
member: BBa_K780003

BBa_K823003
type: ComponentDefinition
persistent identity: BBa K823003
display id: BBa_K823003
version: 1
was generated by: iGEM to SBOL conversion
description: Pveg
created: 2012-07-15T11:00:00Z
modified: 2015-05-08T01:13:29Z
mutable provenance: Bacillus subtilis
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Pveg is a strong, constitutive promoter of Bacillus subtilis
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 0
owner id: 12081
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: no considerations
star: false
creator: Korinna Kraft
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: promoter
sequence: https://synbiohub.org/public/igem/BBa_K823003_sequence/1

promoter
type: SequenceAnnotation
persistent identity: annotation2190180
display id: annotation2190180
version: 1
top level: BBa_K823003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K823003/annotation2190180/range2190180/1

https://synbiohub.org/public/igem/BBa_K823003/annotation2190180/range2190180/1
type: Range
persistent identity: range2190180
display id: range2190180
version: 1
top level: BBa_K823003
direction: direction/forward
start: 1
end: 237
orientation: inline

BBa_K090504
type: ComponentDefinition
persistent identity: BBa K090504
display id: BBa_K090504
version: 1
was generated by: iGEM to SBOL conversion
description: Gram-Positive Strong Constitutive Promoter
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:08:37Z
mutable provenance: We PCRed this part out of the pAD43-25 gram positive vector, acquired from the Bacillus Genetic Stock Center (bgsc.org), catalog number ECE166.
owned by: james
owned by: myers
mutable description: This is a strong constitutive (always-on) promoter for use with gram-positive organisms. It requires a downstream gram-positive ribosomal binding site and coding sequence to function. We only have experience using this part in B. subtilis, but it should function in most gram-positive organisms. It has not been tested and will most likely not work in E. coli. The promoter comes from the cis-regulatory region of the uracil phosphatase in Bacillus cereus. We functionally annotated the promoter ourselves using the DBTBS database motif search feature. (dbtbs.hgc.jp)
discontinued: false
dominant: false
group u list: _188_
m user id: 0
owner id: 3501
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: This part generates high expression in gram-positive organisms, and it may be possible to generate deleterious effects on the cell if used to express certain protein products.
star: false
creator: Daniel Goodman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: putative ComK binding region
sequence annotation: putative SigA -35
sequence annotation: putative SigA -10
sequence: https://synbiohub.org/public/igem/BBa_K090504_sequence/1

putative ComK binding region
type: SequenceAnnotation
persistent identity: annotation1991955
display id: annotation1991955
version: 1
top level: BBa_K090504
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K090504/annotation1991955/range1991955/1

https://synbiohub.org/public/igem/BBa_K090504/annotation1991955/range1991955/1
type: Range
persistent identity: range1991955
display id: range1991955
version: 1
top level: BBa_K090504
direction: direction/forward
start: 76
end: 88
orientation: inline

putative SigA -35
type: SequenceAnnotation
persistent identity: annotation1991956
display id: annotation1991956
version: 1
top level: BBa_K090504
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K090504/annotation1991956/range1991956/1

https://synbiohub.org/public/igem/BBa_K090504/annotation1991956/range1991956/1
type: Range
persistent identity: range1991956
display id: range1991956
version: 1
top level: BBa_K090504
direction: direction/forward
start: 102
end: 108
orientation: inline

putative SigA -10
type: SequenceAnnotation
persistent identity: annotation1991957
display id: annotation1991957
version: 1
top level: BBa_K090504
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K090504/annotation1991957/range1991957/1

https://synbiohub.org/public/igem/BBa_K090504/annotation1991957/range1991957/1
type: Range
persistent identity: range1991957
display id: range1991957
version: 1
top level: BBa_K090504
direction: direction/forward
start: 125
end: 130
orientation: inline

PgsiB
type: ComponentDefinition
persistent identity: BBa K143011
display id: BBa_K143011
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter gsiB for B. subtilis
created: 2008-09-10T11:00:00Z
modified: 2015-05-08T01:10:23Z
mutable provenance: The part was designed using the sequence from the ''B.subtilis'' genome and from previously published papers 23. This sequence was then synthesised by Geneart.
owned by: james
owned by: myers
mutable description: Promoter gsiB is a sigma factor B dependent promoter found in ''B.subtilis''. In ''B.subtilis'' endogenous sigma factor B is activated under mild stress. These mild stress conditions can be generally split into nutrient stress response and physical stress response. Nutrient stress response is triggered by low levels of ATP and GTP and physical stress response is triggered by exposure to blue light, salt, heat, acid or ethanol1. The promoter gsiB has been used previously as a read out for the activation of sigma factor B 2. The context with which we used the promoter gsiB, was to take blue light as an input and give '''Polymerase Per Second'''(PoPS) as an output. To do this the other potential inputs need to be carefully controlled so that only blue light activated the sigma B and gives a PoPS output. In order to get sufficient sigma B activation by blue light the light receptor YtvA, part...., needs to be over expressed in ''B.subtilis'' 3.
discontinued: false
dominant: false
experience: experience/None
group u list: _199_
m user id: 0
owner id: 2090
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Biobrick standard was applied to the promoter gsiB sequence.
star: false
creator: James Chappell
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Sigma B -35
sequence annotation: Sigma B -10
sequence: https://synbiohub.org/public/igem/BBa_K143011_sequence/1

Sigma B -35
type: SequenceAnnotation
persistent identity: annotation1975702
display id: annotation1975702
version: 1
top level: PgsiB
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K143011/annotation1975702/range1975702/1

https://synbiohub.org/public/igem/BBa_K143011/annotation1975702/range1975702/1
type: Range
persistent identity: range1975702
display id: range1975702
version: 1
top level: PgsiB
direction: direction/forward
start: 6
end: 11
orientation: inline

Sigma B -10
type: SequenceAnnotation
persistent identity: annotation1975703
display id: annotation1975703
version: 1
top level: PgsiB
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K143011/annotation1975703/range1975703/1

https://synbiohub.org/public/igem/BBa_K143011/annotation1975703/range1975703/1
type: Range
persistent identity: range1975703
display id: range1975703
version: 1
top level: PgsiB
direction: direction/forward
start: 25
end: 30
orientation: inline

Pctc
type: ComponentDefinition
persistent identity: BBa K143010
display id: BBa_K143010
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter ctc for B. subtilis
created: 2008-09-10T11:00:00Z
modified: 2015-05-08T01:10:23Z
mutable provenance: The part was designed using the sequence from the ''B.subtilis'' genome and from previously published papers 23. This sequence was then synthesised by Geneart.
owned by: james
owned by: myers
mutable description: Promoter ctc is a sigma factor B dependent promoter found in ''B.subtilis''. In ''B.subtilis'' endogenous sigma factor B is activated under mild stress. These mild stress conditions can be generally split into nutrient stress response and physical stress response. Nutrient stress response is triggered by low levels of ATP and GTP and physical stress response is triggered by exposure to blue light, salt, heat, acid or ethanol1. The promoter ctc has been used previously as a read out for the activation of sigma factor B 2. *The context with which we used the promoter ctc, was to take blue light as an input and give '''Polymerase Per Second'''(PoPS) as an output. To do this the other potential inputs need to be carefully controlled so that only blue light activated the sigma B and gives a PoPS output. In order to get sufficient sigma B activation by blue light the light receptor YtvA, part...., needs to be over expressed in ''B.subtilis'' 3. ===Reference=== <biblio> #1 pmid=16267279 #2 pmid=3100810 #3 pmid=17575448 </biblio>
discontinued: false
dominant: false
experience: experience/Works
group u list: _199_
m user id: 0
owner id: 2090
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Biobrick standard was applied to the promoter ctc sequence.
star: false
creator: James Chappell
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Sigma B -10
sequence annotation: Sigma B -35
sequence: https://synbiohub.org/public/igem/BBa_K143010_sequence/1

Sigma B -10
type: SequenceAnnotation
persistent identity: annotation1975700
display id: annotation1975700
version: 1
top level: Pctc
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K143010/annotation1975700/range1975700/1

https://synbiohub.org/public/igem/BBa_K143010/annotation1975700/range1975700/1
type: Range
persistent identity: range1975700
display id: range1975700
version: 1
top level: Pctc
direction: direction/forward
start: 41
end: 46
orientation: inline

Sigma B -35
type: SequenceAnnotation
persistent identity: annotation1975701
display id: annotation1975701
version: 1
top level: Pctc
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K143010/annotation1975701/range1975701/1

https://synbiohub.org/public/igem/BBa_K143010/annotation1975701/range1975701/1
type: Range
persistent identity: range1975701
display id: range1975701
version: 1
top level: Pctc
direction: direction/forward
start: 21
end: 28
orientation: inline

Ph-s
type: ComponentDefinition
persistent identity: BBa K143015
display id: BBa_K143015
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter hyper-spank for B. subtilis
created: 2008-09-17T11:00:00Z
modified: 2015-05-08T01:10:23Z
mutable provenance: The part was designed using the sequence from the ''B.subtilis'' genome and from previously published papers 123. This sequence was then synthesised by Geneart.
owned by: james
owned by: myers
mutable description: Promoter hyper-spank is an inducible promoter that has been designed for high expression in ''B.subtilis''. Gene expression under the promoter hyper-spank can be induced by addition of Isopropyl β-D-1-thiogalactopyranoside (IPTG). The context with which we used the promoter hyper-spank, was to take an input of IPTG and give '''Polymerase Per Second'''(PoPS) as an output. IPTG does not induce the promoter hyper-spank directly, but requires the transcriptional regulator '''LacI''', (<bbpart>BBa_K413035</bbpart>). This means that LacI must be constitutively expressed in ''B.subtilis'' in order to use the promoter hyper-spank.
discontinued: false
dominant: false
experience: experience/Works
group u list: _199_
m user id: 0
owner id: 3475
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Biobrick standard was applied to the promoter hyper-spank sequence.
star: false
creator: Chris Hirst
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: LacI Operator
sequence annotation: Sigma A -10
sequence annotation: Sigma A -35
sequence: https://synbiohub.org/public/igem/BBa_K143015_sequence/1

LacI Operator
type: SequenceAnnotation
persistent identity: annotation1976423
display id: annotation1976423
version: 1
top level: Ph-s
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K143015/annotation1976423/range1976423/1

https://synbiohub.org/public/igem/BBa_K143015/annotation1976423/range1976423/1
type: Range
persistent identity: range1976423
display id: range1976423
version: 1
top level: Ph-s
direction: direction/forward
start: 10
end: 30
orientation: inline

Sigma A -10
type: SequenceAnnotation
persistent identity: annotation1976425
display id: annotation1976425
version: 1
top level: Ph-s
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K143015/annotation1976425/range1976425/1

https://synbiohub.org/public/igem/BBa_K143015/annotation1976425/range1976425/1
type: Range
persistent identity: range1976425
display id: range1976425
version: 1
top level: Ph-s
direction: direction/forward
start: 69
end: 74
orientation: inline

LacI Operator
type: SequenceAnnotation
persistent identity: annotation1976426
display id: annotation1976426
version: 1
top level: Ph-s
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K143015/annotation1976426/range1976426/1

https://synbiohub.org/public/igem/BBa_K143015/annotation1976426/range1976426/1
type: Range
persistent identity: range1976426
display id: range1976426
version: 1
top level: Ph-s
direction: direction/forward
start: 81
end: 101
orientation: inline

Sigma A -35
type: SequenceAnnotation
persistent identity: annotation1976424
display id: annotation1976424
version: 1
top level: Ph-s
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K143015/annotation1976424/range1976424/1

https://synbiohub.org/public/igem/BBa_K143015/annotation1976424/range1976424/1
type: Range
persistent identity: range1976424
display id: range1976424
version: 1
top level: Ph-s
direction: direction/forward
start: 46
end: 50
orientation: inline

Pxyl
type: ComponentDefinition
persistent identity: BBa K143014
display id: BBa_K143014
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter Xyl for B.subtilis
created: 2008-09-14T11:00:00Z
modified: 2015-05-08T01:10:23Z
mutable provenance: The part was designed using the sequence from the ''B.subtilis'' genome and from previously published papers 12. This sequence was then synthesised by Geneart.
owned by: james
owned by: myers
mutable description: Promoter Xylose is an inducible promoter that has been designed for high expression in ''B.subtilis''. Gene expression under the promoter xylose can be induced by addition of xylose. The context with which we used the promoter xylose, was to take an input of xylose and give '''Polymerase Per Second'''(PoPS) as an output. Promoter xylose is an inducible promoter that has been designed for high expression in ''B.subtilis''. Gene expression under the promoter xylose can be induced by addition of xylose. The context with which we used the promoter xylose, was to take an input of xylose and give '''Polymerase Per Second'''(PoPS) as an output. Xylose does not induce the promoter xylose directly, but requires the transcriptional regulator '''XylR''', (<bbpart>BBa_K143036</bbpart>) This means that XylR must be constitutively expressed in ''B.subtilis'' in order to use the promoter xylose.
discontinued: false
dominant: false
experience: experience/None
group u list: _199_
m user id: 0
owner id: 2090
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Biobrick standard was applied to the promoter xylose sequence.
star: false
creator: James Chappell
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: XylR Operator
sequence annotation: Sigma A -10
sequence annotation: Sigma A -35
sequence: https://synbiohub.org/public/igem/BBa_K143014_sequence/1

XylR Operator
type: SequenceAnnotation
persistent identity: annotation1975869
display id: annotation1975869
version: 1
top level: Pxyl
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K143014/annotation1975869/range1975869/1

https://synbiohub.org/public/igem/BBa_K143014/annotation1975869/range1975869/1
type: Range
persistent identity: range1975869
display id: range1975869
version: 1
top level: Pxyl
direction: direction/forward
start: 65
end: 75
orientation: inline

Sigma A -10
type: SequenceAnnotation
persistent identity: annotation1976429
display id: annotation1976429
version: 1
top level: Pxyl
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K143014/annotation1976429/range1976429/1

https://synbiohub.org/public/igem/BBa_K143014/annotation1976429/range1976429/1
type: Range
persistent identity: range1976429
display id: range1976429
version: 1
top level: Pxyl
direction: direction/forward
start: 36
end: 41
orientation: inline

XylR Operator
type: SequenceAnnotation
persistent identity: annotation1975868
display id: annotation1975868
version: 1
top level: Pxyl
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K143014/annotation1975868/range1975868/1

https://synbiohub.org/public/igem/BBa_K143014/annotation1975868/range1975868/1
type: Range
persistent identity: range1975868
display id: range1975868
version: 1
top level: Pxyl
direction: direction/forward
start: 51
end: 61
orientation: inline

Sigma A -35
type: SequenceAnnotation
persistent identity: annotation1976428
display id: annotation1976428
version: 1
top level: Pxyl
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K143014/annotation1976428/range1976428/1

https://synbiohub.org/public/igem/BBa_K143014/annotation1976428/range1976428/1
type: Range
persistent identity: range1976428
display id: range1976428
version: 1
top level: Pxyl
direction: direction/forward
start: 13
end: 18
orientation: inline

Pveg
type: ComponentDefinition
persistent identity: BBa K143012
display id: BBa_K143012
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter veg a constitutive promoter for B. subtilis
created: 2008-09-10T11:00:00Z
modified: 2015-05-08T01:10:23Z
mutable provenance: The Pveg promoter was suggested to us by Dr. Jan-Willem Veening of Newcastle University. This sequence supplied was compared to that of the DBTBS database#3 then a section containing the binding site synthesised by Geneart.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Pveg is a constitutive promoter that constitutively expresses the P43 protein in ''B.subtilis''. Pveg contains binding sites for the ''B.sutbilis'' major sigma factor#1. Pveg in ''B.subtilis'' utilises two binding sites to cause high expression of genes#2, however our Pveg is lacking the upstream site to give a medium level of gene expression. It has been noted that the sporulation master regulatoion factor spoOA interacts with Pveg though it is not known how#3. The context with which we used the promoter Pveg is as a '''Polymerase Per Second''' (PoPS) generator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _199_
m user id: 0
owner id: 2090
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The biobrick part was designed to include a single binding site for the ''B.subtilis major sigma factor. In addition the biobrick standard was applied to the promoter Pveg sequence.
star: false
creator: James Chappell
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Sigma A-35
sequence annotation: Sigma A -10
sequence: https://synbiohub.org/public/igem/BBa_K143012_sequence/1

Sigma A-35
type: SequenceAnnotation
persistent identity: annotation1975704
display id: annotation1975704
version: 1
top level: Pveg
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K143012/annotation1975704/range1975704/1

https://synbiohub.org/public/igem/BBa_K143012/annotation1975704/range1975704/1
type: Range
persistent identity: range1975704
display id: range1975704
version: 1
top level: Pveg
direction: direction/forward
start: 63
end: 68
orientation: inline

Sigma A -10
type: SequenceAnnotation
persistent identity: annotation1975705
display id: annotation1975705
version: 1
top level: Pveg
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K143012/annotation1975705/range1975705/1

https://synbiohub.org/public/igem/BBa_K143012/annotation1975705/range1975705/1
type: Range
persistent identity: range1975705
display id: range1975705
version: 1
top level: Pveg
direction: direction/forward
start: 86
end: 91
orientation: inline

BBa_K780003
type: ComponentDefinition
persistent identity: BBa K780003
display id: BBa_K780003
version: 1
was generated by: iGEM to SBOL conversion
description: Strong constitutive promoter for Bacillus subtilis
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:18Z
mutable provenance: 'host-takover module' from B.subtilis Bacteriophage SPO1
owned by: james
owned by: myers
mutable description: Strong constitutive promoter for Bacillus subtilis
discontinued: false
dominant: false
experience: experience/None
group u list: _1034_
m user id: 0
owner id: 10113
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: -
star: false
creator: Elżbieta Jankowska
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K780003_sequence/1

BBa_K090501
type: ComponentDefinition
persistent identity: BBa K090501
display id: BBa_K090501
version: 1
was generated by: iGEM to SBOL conversion
description: Gram-Positive IPTG-Inducible Promoter
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:08:37Z
mutable provenance: We PCRed this part out of the pMUTIN-YFP gram positive vector, acquired from the Bacillus Genetic Stock Center (bgsc.org), catalog number ECE151.
owned by: james
owned by: myers
mutable description: This is an IPTG-inducible promoter for use with gram-positive organisms. It requires a downstream gram-positive ribosomal binding site and coding sequence to function. We only have experience using this part in B. subtilis, but it should function in most gram-positive organisms. It has not been tested and will most likely not work in E. coli.
discontinued: false
dominant: false
experience: experience/None
group u list: _188_
m user id: 0
owner id: 3501
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: We found the functional annotation in the associated paper: Kaltwasser M., T. Wiegert, and W. Schumann. 2002. Construction and Application of Epitope- and Green Fluorescent Protein-Tagging Integration Vectors for Bacillus subtilis. Appl. Environ. Microbiol 68:2624-2628 The primers we used to extract it (sans biobrick ends) are as follow: Forward: AGAACAACCTCTGCTAAAATTC Reverse: AAGCTTAATTGTTATCCGCTC
star: false
creator: Daniel Goodman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: lacOid Operator
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_K090501_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation1991823
display id: annotation1991823
version: 1
top level: BBa_K090501
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K090501/annotation1991823/range1991823/1

https://synbiohub.org/public/igem/BBa_K090501/annotation1991823/range1991823/1
type: Range
persistent identity: range1991823
display id: range1991823
version: 1
top level: BBa_K090501
direction: direction/forward
start: 66
end: 71
orientation: inline

lacOid Operator
type: SequenceAnnotation
persistent identity: annotation1991824
display id: annotation1991824
version: 1
top level: BBa_K090501
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K090501/annotation1991824/range1991824/1

https://synbiohub.org/public/igem/BBa_K090501/annotation1991824/range1991824/1
type: Range
persistent identity: range1991824
display id: range1991824
version: 1
top level: BBa_K090501
direction: direction/forward
start: 78
end: 97
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1991822
display id: annotation1991822
version: 1
top level: BBa_K090501
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K090501/annotation1991822/range1991822/1

https://synbiohub.org/public/igem/BBa_K090501/annotation1991822/range1991822/1
type: Range
persistent identity: range1991822
display id: range1991822
version: 1
top level: BBa_K090501
direction: direction/forward
start: 43
end: 48
orientation: inline

BBa_K823000
type: ComponentDefinition
persistent identity: BBa K823000
display id: BBa_K823000
version: 1
was generated by: iGEM to SBOL conversion
description: PliaG
created: 2012-07-15T11:00:00Z
modified: 2015-05-08T01:13:29Z
mutable provenance: Bacillus subtilis
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Promoter of the liaG gene of Bacillus subtilis, weak constitutive
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 0
owner id: 12081
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: no considerations
star: false
creator: Korinna Kraft
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10 box
sequence annotation: -35 box
sequence annotation: PliaG
sequence: https://synbiohub.org/public/igem/BBa_K823000_sequence/1

-10 box
type: SequenceAnnotation
persistent identity: annotation2182555
display id: annotation2182555
version: 1
top level: BBa_K823000
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823000/annotation2182555/range2182555/1

https://synbiohub.org/public/igem/BBa_K823000/annotation2182555/range2182555/1
type: Range
persistent identity: range2182555
display id: range2182555
version: 1
top level: BBa_K823000
direction: direction/forward
start: 105
end: 110
orientation: inline

-35 box
type: SequenceAnnotation
persistent identity: annotation2182554
display id: annotation2182554
version: 1
top level: BBa_K823000
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823000/annotation2182554/range2182554/1

https://synbiohub.org/public/igem/BBa_K823000/annotation2182554/range2182554/1
type: Range
persistent identity: range2182554
display id: range2182554
version: 1
top level: BBa_K823000
direction: direction/forward
start: 81
end: 86
orientation: inline

PliaG
type: SequenceAnnotation
persistent identity: annotation2182553
display id: annotation2182553
version: 1
top level: BBa_K823000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K823000/annotation2182553/range2182553/1

https://synbiohub.org/public/igem/BBa_K823000/annotation2182553/range2182553/1
type: Range
persistent identity: range2182553
display id: range2182553
version: 1
top level: BBa_K823000
direction: direction/forward
start: 1
end: 121
orientation: inline

BBa_K823002
type: ComponentDefinition
persistent identity: BBa K823002
display id: BBa_K823002
version: 1
was generated by: iGEM to SBOL conversion
description: PlepA
created: 2012-07-15T11:00:00Z
modified: 2015-05-08T01:13:29Z
mutable provenance: Bacillus subtilis
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: PlepA is the promoter of the PlepA gene of Bacillus subtilis. It is a constitutive promoter and does not contain a ribosome binding site.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 0
owner id: 12081
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: no considerations
star: false
creator: Korinna Kraft
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K823002_sequence/1

P43
type: ComponentDefinition
persistent identity: BBa K143013
display id: BBa_K143013
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter 43 a constitutive promoter for B. subtilis
created: 2008-09-10T11:00:00Z
modified: 2015-05-08T01:10:23Z
mutable provenance: The part was designed using the sequence from the ''B.subtilis'' genome and from previously published papers 2. This sequence was then synthesised by Geneart.
owned by: james
owned by: myers
mutable description: Promoter 43 is a constitutive promoter that constitutively expresses the P43 protein in ''B.subtilis''. This promoter has been shown to be recognized and active during the exponential and lag phases of growth. It has been hypothesized that the ability to recognize the promoter in exponential and lag phase of growth is due to the recognition of the promoter by both sigma factor 55 (the major sigma factor) and sigma factor 37 (the lag phase sigma factor) 1. The P43 promoter has been previously used for constitutive expression of exogenous genes within ''B.subtilis'' vectors 2. The context with which we used the promoter P43 is as a '''Polymerase Per Second''' (PoPS) generator.
discontinued: false
dominant: false
experience: experience/None
group u list: _199_
m user id: 0
owner id: 2090
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The biobrick part was designed to include the binding sites for both the sigma factor A and B. In addition the biobrick standard was applied to the promoter P43 sequence.
star: false
creator: James Chappell
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Sigma A -35
sequence annotation: Sigma B -10
sequence annotation: Sigma B -35
sequence annotation: Sigma A -10
sequence: https://synbiohub.org/public/igem/BBa_K143013_sequence/1

Sigma A -35
type: SequenceAnnotation
persistent identity: annotation1975708
display id: annotation1975708
version: 1
top level: P43
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K143013/annotation1975708/range1975708/1

https://synbiohub.org/public/igem/BBa_K143013/annotation1975708/range1975708/1
type: Range
persistent identity: range1975708
display id: range1975708
version: 1
top level: P43
direction: direction/forward
start: 24
end: 29
orientation: inline

Sigma B -10
type: SequenceAnnotation
persistent identity: annotation1975707
display id: annotation1975707
version: 1
top level: P43
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K143013/annotation1975707/range1975707/1

https://synbiohub.org/public/igem/BBa_K143013/annotation1975707/range1975707/1
type: Range
persistent identity: range1975707
display id: range1975707
version: 1
top level: P43
direction: direction/forward
start: 38
end: 47
orientation: inline

Sigma B -35
type: SequenceAnnotation
persistent identity: annotation1975706
display id: annotation1975706
version: 1
top level: P43
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K143013/annotation1975706/range1975706/1

https://synbiohub.org/public/igem/BBa_K143013/annotation1975706/range1975706/1
type: Range
persistent identity: range1975706
display id: range1975706
version: 1
top level: P43
direction: direction/forward
start: 14
end: 22
orientation: inline

Sigma A -10
type: SequenceAnnotation
persistent identity: annotation1975709
display id: annotation1975709
version: 1
top level: P43
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K143013/annotation1975709/range1975709/1

https://synbiohub.org/public/igem/BBa_K143013/annotation1975709/range1975709/1
type: Range
persistent identity: range1975709
display id: range1975709
version: 1
top level: P43
direction: direction/forward
start: 47
end: 52
orientation: inline

BBa_K823001
type: ComponentDefinition
persistent identity: BBa K823001
display id: BBa_K823001
version: 1
was generated by: iGEM to SBOL conversion
description: PliaI promoter of Bacillus subtilis
created: 2012-07-15T11:00:00Z
modified: 2015-05-08T01:13:29Z
mutable provenance: Bacillus subtilis
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: PliaI is the promoter of the PliaI gene of Bacillus subtilis, which is inducible by bacitracin. It does not contain a ribosome binding site.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 0
owner id: 12081
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: no considerations
star: false
creator: Korinna Kraft
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: -35
sequence annotation: PliaI
sequence annotation: liaR-binding
sequence annotation: +1
sequence: https://synbiohub.org/public/igem/BBa_K823001_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation2202720
display id: annotation2202720
version: 1
top level: BBa_K823001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823001/annotation2202720/range2202720/1

https://synbiohub.org/public/igem/BBa_K823001/annotation2202720/range2202720/1
type: Range
persistent identity: range2202720
display id: range2202720
version: 1
top level: BBa_K823001
direction: direction/forward
start: 185
end: 190
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2202721
display id: annotation2202721
version: 1
top level: BBa_K823001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823001/annotation2202721/range2202721/1

https://synbiohub.org/public/igem/BBa_K823001/annotation2202721/range2202721/1
type: Range
persistent identity: range2202721
display id: range2202721
version: 1
top level: BBa_K823001
direction: direction/forward
start: 160
end: 165
orientation: inline

PliaI
type: SequenceAnnotation
persistent identity: annotation2202715
display id: annotation2202715
version: 1
top level: BBa_K823001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K823001/annotation2202715/range2202715/1

https://synbiohub.org/public/igem/BBa_K823001/annotation2202715/range2202715/1
type: Range
persistent identity: range2202715
display id: range2202715
version: 1
top level: BBa_K823001
direction: direction/forward
start: 1
end: 209
orientation: inline

liaR-binding
type: SequenceAnnotation
persistent identity: annotation2202717
display id: annotation2202717
version: 1
top level: BBa_K823001
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823001/annotation2202717/range2202717/1

https://synbiohub.org/public/igem/BBa_K823001/annotation2202717/range2202717/1
type: Range
persistent identity: range2202717
display id: range2202717
version: 1
top level: BBa_K823001
direction: direction/forward
start: 125
end: 159
orientation: inline

+1
type: SequenceAnnotation
persistent identity: annotation2202718
display id: annotation2202718
version: 1
top level: BBa_K823001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823001/annotation2202718/range2202718/1

https://synbiohub.org/public/igem/BBa_K823001/annotation2202718/range2202718/1
type: Range
persistent identity: range2202718
display id: range2202718
version: 1
top level: BBa_K823001
direction: direction/forward
start: 197
end: 197
orientation: inline

BBa_K1074013
type: ComponentDefinition
persistent identity: BBa K1074013
display id: BBa_K1074013
version: 1
was generated by: iGEM to SBOL conversion
description: promoter sdpR/I
created: 2013-09-15T11:00:00Z
modified: 2015-06-08T02:58:23Z
mutable provenance: from the genome of Bacillus subtilis WB800N
owned by: james
owned by: myers
mutable description: This part is a sigma factor A-dependent promotor of the gene sdpR derived from the B.subtilis ,and it contains an operon for the signaling pathway of sdpc,a bacterial toxin of B.subtilis. It's activated as the toxin sdpC existed,while the natural pructrue of the promotor, sdpR/I ,confers immunity to the SdpC toxin. Cells of B. subtilis enter the pathway to sporulate under conditions of nutrient limitation could generate sdpC, then this promotor would be activated. We use this part to overexpress sdpC toxin in spore-forming cells,to build a self-killing system in B.subtilis for safety thinking.
discontinued: false
dominant: false
group u list: _1383_
m user id: 4206
owner id: 16001
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: NO
star: false
creator: Changlong Zhao
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1074013_sequence/1

https://synbiohub.org/public/igem/BBa_K143015_sequence/1
type: Sequence
persistent identity: BBa K143015 sequence
display id: BBa_K143015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctcgagggtaaatgtgagcactcacaattcattttgcaaaagttgttgactttatctacaaggtgtggcataatgtgtgtaattgtgagcggataacaatt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K090501_sequence/1
type: Sequence
persistent identity: BBa K090501 sequence
display id: BBa_K090501_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agaacaacctctgctaaaattcctgaaaaattttgcaaaaagttgttgactttatctacaaggtgtggcataatgtgtggaattgtgagcggataacaattaagctt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K780003_sequence/1
type: Sequence
persistent identity: BBa K780003 sequence
display id: BBa_K780003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacaaattgcagtaggcatgacaaaatggactca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K143012_sequence/1
type: Sequence
persistent identity: BBa K143012 sequence
display id: BBa_K143012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattttgtcaaaataattttattgacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggctcgtgttgtacaataaatgt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1074013_sequence/1
type: Sequence
persistent identity: BBa K1074013 sequence
display id: BBa_K1074013_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gggttgtttttaaaaaaattcaagttataatgaaaataatacatttatacaaatatctaaatgtctaaatgttttt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K090504_sequence/1
type: Sequence
persistent identity: BBa K090504 sequence
display id: BBa_K090504_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atttttaaagtatgtatacaaatgatgaataaattttggcgatataatgaaggatacagctcccataattggtaaagatactagatagattcatcgtaaaatcatgattttgccaaatttgcccttgaatattagtagcgttttctttacaatcgtaaatagtgtaaaaaagcgtgcaaacgcatgaatatcatctaaaggagagattcacatgggaaaactgtatgtatttgatcctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K143011_sequence/1
type: Sequence
persistent identity: BBa K143011 sequence
display id: BBa_K143011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgtttgtttaaaagaattgtgagcgggaatacaacaac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K823003_sequence/1
type: Sequence
persistent identity: BBa K823003 sequence
display id: BBa_K823003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggagttctgagaattggtatgccttataagtccaattaacagttgaaaacctgcataggagagctatgcgggttttttattttacataatgatacataatttaccgaaacttgcggaacataattgaggaatcatagaattttgtcaaaataattttattgacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggctcgtgttgtacaataaatgtagt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K143014_sequence/1
type: Sequence
persistent identity: BBa K143014 sequence
display id: BBa_K143014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctaaaaaaaatattgaaaatactgacgaggttatataagatgaaaataagttagtttgtttaaacaacaaactaataggtga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K143013_sequence/1
type: Sequence
persistent identity: BBa K143013 sequence
display id: BBa_K143013_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attttacatttttagaaatgggcgtgaaaaaaagcgcgcgattatgtaaaatataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K823001_sequence/1
type: Sequence
persistent identity: BBa K823001 sequence
display id: BBa_K823001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attggccaaagcagaaaggtccgacctaattaaagaaagggaagcaagtgttcatctgtaaagggttttaaaacgccatgcctcgtgcatggcgtttttttgtgccaatgggtccggtgcgagatacgactccggtcttatataaaaatcaatctctgattcgttttgcatatcttccaacttgtataagatgaagacaaggaaaacga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K823000_sequence/1
type: Sequence
persistent identity: BBa K823000 sequence
display id: BBa_K823000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caaaaatcagaccagacaaaagcggcaaatgaataagcggaacggggaaggatttgcggtcaagtccttcccttccgcacgtatcaattcgcaagcttttcctttataatagaatgaatga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K143010_sequence/1
type: Sequence
persistent identity: BBa K143010 sequence
display id: BBa_K143010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cagctaaaccatttttcgaggtttaaatccttatcgttatgggtattgtttgtaat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K823002_sequence/1
type: Sequence
persistent identity: BBa K823002 sequence
display id: BBa_K823002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agtcaatgtatgaatggatacgggatatgaatcaataagtacgtgaaagagaaaagcaacccagatatgatagggaacttttctctttcttgttttacattgaatctttacaatcctattgatataatctaagctagtgtattttgcgtttaatagt
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z