Iron sensitive promoter (test delete later)
This is a promoter that is sensitive to iron (Fe++) concentration (test only)
made a base substitution to remove a EcoRI site
Ore is a consensus sequence localized in some promoters that respond to oleic acid concentration. Its sequence is CGGN3TNA/(R)N8-12CCG
We amplificated this part from p2UG2XORE plasmid
This is a composite part containing the minimal promoter mCYC preceded by multiple consecutive binding sites for the Gli1 binding domain. This part contains the sequences for BBa_K165012 and BBa_K165016 but also has some additional DNA.
It was necessary to design primers for use in PCR to remove this part from its host plasmid and transfer it to a standard Biobrick vector.
Composite part that was taken via PCR from a synthetic plasmid containing the sequence. The plasmid came from Lawrence Berkeley National Laboratory.
Stimulated by CafA found in cafein addicted students
Also activated by sucrose Inhibited by nicotine
This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page] <!-- Add more about the biology of this part here
This is the naturally-occurring (possibly stationary-phase only) promoter for the XylR gene (which encodes the transcriptional regulator XylR).
Ore is a consensus sequence localized in some promoters that respond to oleic acid concentration. Its sequence is CGGN3TNA/(R)N8-12CCG. In this case we have 2 ore consensus sequences.
Downstream to ORE sequence there is CYC TATA
We amplified this part from p2UG2XORE plasmid
Transcription Factor Binding Site from Cyanobacterial Pcpc560
PCR using primers with biobrick prefix and surfix, and then cloned into pSB1AC3.
This is a low-level constitutive yeast promoter.
From David Drubin in the Silver Lab at Harvard.
Zif268-HIV binding sites + ADH1 yeast promoter
The Zif268-HIV transcription factor binding sites on the ADH1 constitutive promoter. Transcription regulation should be constitutive plus regulated by Zif268-HIV-binding transcription factors.
Constructed in our lab with parts from Caroline Ajo-Franklin and David Drubin.
This section of DNA contains the sequence from 300 base pairs upstream of the Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase gene to 30 base pairs downstream into the gene from Pseudomonas fluorescens PfO-1. Our hope is that this region of DNA will contain the promoter for the nitrilase gene. At the time that this part was submitted, it was not tested or sequenced yet.
We isolated this segment of DNA from the Pseudomonas fluorescens PfO-1 genome using a PCR reaction.
This section of DNA comes from the Pseudomonas fluorescens PfO-1 genome (NC_007492). The GeneID for the nitrilase gene that we working with is 3715134, and the protein ID for the nitrilase gene is YP_349417.1. We took the region 300 base pairs upstream of the nitrilase gene and 10 base pairs downstream into the gene.
This is the promoter activated by the transcriptional regulator DntR [BB# here] in response to detection of environmental [salicylate].
'''''This does not physically exist as a basic part due to time constraints; it was cloned as part of composite part which incorporates all of the components necessary for expression of a reporter gene under the control of DntR'''''. For this reason, the ends of the supplied sequence may not accurately represent the boundaries of the functional unit.
Cloned from a historical plasmid based on pQF52 carrying genomic sequences from ''Burkholderia cepacia''.
This minimal promoter can be used as a base for designing promoters by adding binding sites upstream.
From David Drubin in the Silver Lab at Harvard
The YY1 transcription factor binding sites on the mCYC minimal promoter. All transcription regulation comes from YY1-binding transcription factors.
Made in our lab with parts from Caroline Ajo-Franklin and David Drubin.
Gli1 binding sites + MET25 inducible yeast promoter
This promoter was isolated by taking 500bp upstream of the MFA2 gene of S. cerevisiae. This promoter will be activated when A factor is expressed in an A haploid yeast cell.
This gene is on the Watson strand of the entered sequence.
This is the naturally-occurring (possibly stationary-phase only) promoter for the DntR gene (which encodes the transcriptional regulator DntR) back-to-back with the DntR-regulated promoter.
This does not physically exist as a basic part due to time constraints; it was cloned as part of composite part which incorporates all of the components necessary for expression of a reporter gene under the control of DntR. For this reason, the ends of the supplied sequence may not accurately represent the boundaries of the functional unit.
Gli1 binding sites + ADH1 constitutive yeast promoter
The Gli1 transcription factor binding sites on the ADH1 constitutive promoter. Transcription regulation should be constitutive plus regulated by YY1-binding transcription factors.
Encodes for oxidoreductase and promotes the reduction/oxidation of iron (FeII and FeII).
Digested using Pst I, Xba I inserted into BBa_E0422.
Promoter region from Acidithiobacillus ferrooxidans rus operon
This is a high level constitutive promoter from the yeast gene TEF2.
From the yeast genome using promoter definition from: Mumberg, Muller, and Funk: http://dx.doi.org/10.1016/0378-1119(95)00037-7
Simon St. Pierre extracted from S. Cervisiae genome.
Rick Carrick, Ambhi Ganesan, Alex McMillan
This promoter was isolated by taking 500bp upstream of the MFA1 gene of S. cerevisiae. This promoter will be activated when A factor is expressed in an A haploid yeast cell.
This promotor is on the crick strand of the entered sequence.
Gli1 binding sites + TEF constitutive yeast promoter
The Gli1 transcription factor binding sites on the TEF2 constitutive promoter. Transcription regulation should be constitutive plus regulated by Gli1-binding transcription factors.
The Gli1 and Zif268-HIV transcription factor binding sites on the mCYC minimal promoter (in that order.) Transcription regulation comes from Gli1 and Zif268-HIV binding transcription factors.
promoter of Alcaligenes eutrophus PhaR gene
PCR using primers with biobrick prefix and surfix, cloned into pSB1AC3, and then eliminate Pst1 using site-directed mutation.
nitrogenase iron protein, vnfH Rhodopseudomonas palustris CGA009
This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. In a Bca1256 plasmid backbone. Our microarray results showed that this gene was upregulated four fold in response to low frequency ultrasound and two fold in response to audible sound. We cloned approximately 400 base pairs upstream from the start codon for the ygeF pseudogene.
promoter of Alcaligenes eutrophus PhaP gene
This promoter encoding for a thiolase involved in beta-oxidation of fatty acids.
This promoter contains one only ora consensus sequence that respond to oleic acid. Its sequence is CGGN3TNA/(R)N8-12CCG
We amplified this part from genomic DNA yeast.
The Gli1 and LexA transcription factor binding sites on the mCYC minimal promoter (in that order.) Transcription regulation comes from Gli1 and LexA-binding transcription factors.