//regulation/positive
type: Collection
persistent identity: regulation positive
display id: regulation_positive
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_K110016
member: BBa_I12036
member: Prm +
member: BBa_K180012
member: pBAD:SDnaK
member: BBa_K1486046
member: BBa_K606039
member: BBa_K1660005
member: VirA/G
member: BBa_K180000
member: BBa_K091156
member: pT7
member: BBa_K110004
member: OmpR
member: p_qrr4
member: BBa_K1946006
member: BBa_K206001
member: BBa_K180007
member: BBa_K427003
member: BBa_K1315005
member: BBa_K116401
member: BBa_K266007
member: BBa_K284002
member: BBa_K284003
member: BBa_K112701
member: BBa_K180006
member: BBa_K566005
member: BBa_K180019
member: BBa_K145150
member: BBa_K1520010
member: BBa_K266000
member: hybB
member: pBAD:CspA
member: LasR+PAI
member: BBa_K136010
member: BBa_K180018
member: pspoIIE
member: BBa_K284008
member: BBa_I13406
member: BBa_K091157
member: BBa_K266006
member: BBa_I765007
member: BBa_K338081
member: pBAD:Cpn60
member: BBa_I13453
member: BBa_I746104
member: BBa_K112402
member: BBa_J06403
member: BBa_K831016
member: BBa_K2110005
member: P1
member: BBa_I746361
member: BBa_I741019
member: BBa_J102001
member: BBa_K1486048
member: Las CIO
member: Pvir
member: HSP-LacI
member: BBa_K338062
member: BBa_I739105
member: BBa_I746363
member: BBa_K180005
member: BBa_K2110007
member: BBa_I741021
member: BBa_K180017
member: BBa_K180010
member: BBa_K1520516
member: BBa_K598001
member: BBa_K165041
member: BBa_J64804

BBa_K110016
type: ComponentDefinition
persistent identity: BBa K110016
display id: BBa_K110016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: A-Cell Promoter STE2 (backwards)
creator: James DiCarlo
created: 2008-08-26T11:00:00Z
modified: 2015-05-08T01:09:09Z
discontinued: false
dominant: false
experience: experience/None
group u list: _201_
m user id: 0
owner id: 2669
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is the same sequence as STE2 promotor(BBa_K110008), but with suffix and prefix switched
mutable notes: This is directly from the DNA sequence
mutable provenance: sgd
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: MATalpha2
sequence annotation: STE2 Promoter
sequence annotation: STE12
sequence annotation: MCM1
sequence: https://synbiohub.org/public/igem/BBa_K110016_sequence/1

MATalpha2
type: SequenceAnnotation
persistent identity: annotation1981091
display id: annotation1981091
version: 1
top level: BBa_K110016
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110016/annotation1981091/range1981091/1

https://synbiohub.org/public/igem/BBa_K110016/annotation1981091/range1981091/1
type: Range
persistent identity: range1981091
display id: range1981091
version: 1
direction: direction/forward
top level: BBa_K110016
start: 273
end: 282
orientation: inline

STE2 Promoter
type: SequenceAnnotation
persistent identity: annotation1981094
display id: annotation1981094
version: 1
top level: BBa_K110016
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K110016/annotation1981094/range1981094/1

https://synbiohub.org/public/igem/BBa_K110016/annotation1981094/range1981094/1
type: Range
persistent identity: range1981094
display id: range1981094
version: 1
direction: direction/forward
top level: BBa_K110016
start: 1
end: 500
orientation: inline

MATalpha2
type: SequenceAnnotation
persistent identity: annotation1981093
display id: annotation1981093
version: 1
top level: BBa_K110016
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110016/annotation1981093/range1981093/1

https://synbiohub.org/public/igem/BBa_K110016/annotation1981093/range1981093/1
type: Range
persistent identity: range1981093
display id: range1981093
version: 1
direction: direction/forward
top level: BBa_K110016
start: 295
end: 302
orientation: inline

STE12
type: SequenceAnnotation
persistent identity: annotation1981095
display id: annotation1981095
version: 1
top level: BBa_K110016
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110016/annotation1981095/range1981095/1

https://synbiohub.org/public/igem/BBa_K110016/annotation1981095/range1981095/1
type: Range
persistent identity: range1981095
display id: range1981095
version: 1
direction: direction/forward
top level: BBa_K110016
start: 304
end: 311
orientation: inline

MCM1
type: SequenceAnnotation
persistent identity: annotation1981092
display id: annotation1981092
version: 1
top level: BBa_K110016
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110016/annotation1981092/range1981092/1

https://synbiohub.org/public/igem/BBa_K110016/annotation1981092/range1981092/1
type: Range
persistent identity: range1981092
display id: range1981092
version: 1
direction: direction/forward
top level: BBa_K110016
start: 284
end: 293
orientation: inline

BBa_K1520515
type: ComponentDefinition
persistent identity: BBa K1520515
display id: BBa_K1520515
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MC7-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter-Prlux-rbs-OMPA-golB-rbs-luxI-Ter.
creator: yue wu
created: 2014-09-22T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: MC7-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter-Prlux-rbs-OMPA-golB-rbs-luxI-Ter. It is a test of our positive feedback circuit for microcystin.
mutable notes: None
mutable provenance: MC7, luxI, Pcons2, luxR, Prlux, OMPA-golB, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1520507
component: BBa_K1520029
component: BBa_K1520003
component: BBa_K1520504
component: BBa_K1520001
component: BBa_R0062
sequence annotation: BBa_K1520504
sequence annotation: BBa_K1520003
sequence annotation: BBa_K1520507
sequence annotation: BBa_K1520029
sequence annotation: BBa_R0062
sequence annotation: BBa_K1520001
sequence: https://synbiohub.org/public/igem/BBa_K1520515_sequence/1

BBa_K1520507
type: Component
persistent identity: component2385687
display id: component2385687
version: 1
top level: BBa_K1520515
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520507/1

BBa_K1520029
type: Component
persistent identity: component2385691
display id: component2385691
version: 1
top level: BBa_K1520515
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520029/1

BBa_K1520003
type: Component
persistent identity: component2385675
display id: component2385675
version: 1
top level: BBa_K1520515
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520003/1

BBa_K1520504
type: Component
persistent identity: component2385668
display id: component2385668
version: 1
top level: BBa_K1520515
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520504/1

BBa_K1520504
type: Component
persistent identity: component2385690
display id: component2385690
version: 1
top level: BBa_K1520515
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520504/1

BBa_K1520001
type: Component
persistent identity: component2385665
display id: component2385665
version: 1
top level: BBa_K1520515
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520001/1

BBa_K1520029
type: Component
persistent identity: component2385669
display id: component2385669
version: 1
top level: BBa_K1520515
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520029/1

BBa_R0062
type: Component
persistent identity: component2385677
display id: component2385677
version: 1
top level: BBa_K1520515
access: public
definition: lux pR

BBa_K1520504
type: SequenceAnnotation
persistent identity: annotation2385668
display id: annotation2385668
version: 1
top level: BBa_K1520515
location: https://synbiohub.org/public/igem/BBa_K1520515/annotation2385668/range2385668/1
component: BBa_K1520504

https://synbiohub.org/public/igem/BBa_K1520515/annotation2385668/range2385668/1
type: Range
persistent identity: range2385668
display id: range2385668
version: 1
direction: direction/reverse
top level: BBa_K1520515
start: 142
end: 802
orientation: inline

BBa_K1520003
type: SequenceAnnotation
persistent identity: annotation2385675
display id: annotation2385675
version: 1
top level: BBa_K1520515
location: https://synbiohub.org/public/igem/BBa_K1520515/annotation2385675/range2385675/1
component: BBa_K1520003

https://synbiohub.org/public/igem/BBa_K1520515/annotation2385675/range2385675/1
type: Range
persistent identity: range2385675
display id: range2385675
version: 1
direction: direction/reverse
top level: BBa_K1520515
start: 948
end: 1926
orientation: inline

BBa_K1520504
type: SequenceAnnotation
persistent identity: annotation2385690
display id: annotation2385690
version: 1
top level: BBa_K1520515
location: https://synbiohub.org/public/igem/BBa_K1520515/annotation2385690/range2385690/1
component: BBa_K1520504

https://synbiohub.org/public/igem/BBa_K1520515/annotation2385690/range2385690/1
type: Range
persistent identity: range2385690
display id: range2385690
version: 1
direction: direction/reverse
top level: BBa_K1520515
start: 2814
end: 3474
orientation: inline

BBa_K1520507
type: SequenceAnnotation
persistent identity: annotation2385687
display id: annotation2385687
version: 1
top level: BBa_K1520515
location: https://synbiohub.org/public/igem/BBa_K1520515/annotation2385687/range2385687/1
component: BBa_K1520507

https://synbiohub.org/public/igem/BBa_K1520515/annotation2385687/range2385687/1
type: Range
persistent identity: range2385687
display id: range2385687
version: 1
direction: direction/reverse
top level: BBa_K1520515
start: 1996
end: 2805
orientation: inline

BBa_K1520029
type: SequenceAnnotation
persistent identity: annotation2385691
display id: annotation2385691
version: 1
top level: BBa_K1520515
location: https://synbiohub.org/public/igem/BBa_K1520515/annotation2385691/range2385691/1
component: BBa_K1520029

https://synbiohub.org/public/igem/BBa_K1520515/annotation2385691/range2385691/1
type: Range
persistent identity: range2385691
display id: range2385691
version: 1
direction: direction/reverse
top level: BBa_K1520515
start: 3483
end: 3611
orientation: inline

BBa_K1520029
type: SequenceAnnotation
persistent identity: annotation2385669
display id: annotation2385669
version: 1
top level: BBa_K1520515
location: https://synbiohub.org/public/igem/BBa_K1520515/annotation2385669/range2385669/1
component: BBa_K1520029

https://synbiohub.org/public/igem/BBa_K1520515/annotation2385669/range2385669/1
type: Range
persistent identity: range2385669
display id: range2385669
version: 1
direction: direction/reverse
top level: BBa_K1520515
start: 811
end: 939
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2385677
display id: annotation2385677
version: 1
top level: BBa_K1520515
location: https://synbiohub.org/public/igem/BBa_K1520515/annotation2385677/range2385677/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K1520515/annotation2385677/range2385677/1
type: Range
persistent identity: range2385677
display id: range2385677
version: 1
direction: direction/forward
top level: BBa_K1520515
start: 1935
end: 1989
orientation: inline

BBa_K1520001
type: SequenceAnnotation
persistent identity: annotation2385665
display id: annotation2385665
version: 1
top level: BBa_K1520515
location: https://synbiohub.org/public/igem/BBa_K1520515/annotation2385665/range2385665/1
component: BBa_K1520001

https://synbiohub.org/public/igem/BBa_K1520515/annotation2385665/range2385665/1
type: Range
persistent identity: range2385665
display id: range2385665
version: 1
direction: direction/reverse
top level: BBa_K1520515
start: 1
end: 133
orientation: inline

BBa_K1315005
type: ComponentDefinition
persistent identity: BBa K1315005
display id: BBa_K1315005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: The clp gene from Xanthomonas campestris
creator: Dimitrios Michailidis
created: 2014-09-24T11:00:00Z
modified: 2015-05-08T01:09:50Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1690_
m user id: 0
owner id: 21812
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: c-di-GMP Repressed DNA Binding Protein (Clp) Quorum sensing and bacterial cell signalling are mechanisms known to be utilised by bacteria to regulate their virulence. The RpfC-RpfG sensory system has been found to activate the production of virulence factors like extracellular enzymes and polysaccharides in Xanthomonas campestris. It has been also suggested that this system is involved in linking environmental stimuli, including DSF, with pathogenicity in Xanthomonas campestris. DSF is a cis-unsaturated fatty acid from the DSF family. The RpfC-RpfG two-component system is the most well understood one and is present in all Xanthomonads. http://www.ncbi.nlm.nih.gov/pubmed/?term=r+ryan+dsf+family http://www.ncbi.nlm.nih.gov/pubmed/?term=A+two-component+system+involving+an+HD-GYP+domain+protein+links+cell%E2%80%93cell+signalling+to+pathogenicity+gene+expression+in+Xanthomonas+campestris
mutable notes: The sequence had 1 PstI site that was mutated out by site-directed mutagenesis.
mutable provenance: The source of this part is Xanthomonas campestris pv. campestris 8004 genomic DNA which was provided by the Robert Ryan lab in the Molecular Microbiology department of the University of Dundee, UK.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Clp
sequence: https://synbiohub.org/public/igem/BBa_K1315005_sequence/1

Clp
type: SequenceAnnotation
persistent identity: annotation2386527
display id: annotation2386527
version: 1
top level: BBa_K1315005
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1315005/annotation2386527/range2386527/1

https://synbiohub.org/public/igem/BBa_K1315005/annotation2386527/range2386527/1
type: Range
persistent identity: range2386527
display id: range2386527
version: 1
direction: direction/forward
top level: BBa_K1315005
start: 1
end: 696
orientation: inline

BBa_J07007
type: ComponentDefinition
persistent identity: BBa J07007
display id: BBa_J07007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ctx promoter
creator: igem_mit_2005
created: 2005-07-05T11:00:00Z
modified: 2015-08-31T04:08:19Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_12_21_1_
m user id: 0
owner id: 363
owning group id: 21
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: V.cholerae ctx gene promoter region. Transcription takes place In the presence of a ToxR dimer. We don't have the -10 and -35 sites annotated (need to do this).
mutable provenance: GENBANK M21250
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: transcription start
sequence annotation: ctx promoter
sequence annotation: -10 region
sequence: https://synbiohub.org/public/igem/BBa_J07007_sequence/1

transcription start
type: SequenceAnnotation
persistent identity: annotation1577387
display id: annotation1577387
version: 1
top level: BBa_J07007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J07007/annotation1577387/range1577387/1

https://synbiohub.org/public/igem/BBa_J07007/annotation1577387/range1577387/1
type: Range
persistent identity: range1577387
display id: range1577387
version: 1
direction: direction/forward
top level: BBa_J07007
start: 145
end: 145
orientation: inline

ctx promoter
type: SequenceAnnotation
persistent identity: annotation1571612
display id: annotation1571612
version: 1
top level: BBa_J07007
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J07007/annotation1571612/range1571612/1

https://synbiohub.org/public/igem/BBa_J07007/annotation1571612/range1571612/1
type: Range
persistent identity: range1571612
display id: range1571612
version: 1
direction: direction/forward
top level: BBa_J07007
start: 1
end: 145
orientation: inline

-10 region
type: SequenceAnnotation
persistent identity: annotation1579234
display id: annotation1579234
version: 1
top level: BBa_J07007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J07007/annotation1579234/range1579234/1

https://synbiohub.org/public/igem/BBa_J07007/annotation1579234/range1579234/1
type: Range
persistent identity: range1579234
display id: range1579234
version: 1
direction: direction/forward
top level: BBa_J07007
start: 133
end: 138
orientation: inline

BBa_J16101
type: ComponentDefinition
persistent identity: BBa J16101
display id: BBa_J16101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BanAp - Banana-induced Promoter
creator: James Brown
created: 2006-07-23T11:00:00Z
modified: 2015-08-31T04:08:33Z
discontinued: false
dominant: false
group u list: _48_
m user id: 0
owner id: 740
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Taken from no other than Curious George, this promoter is induced by the presence of banana in the metabolism of any mammal. It is very weak however without the presence of the HngR protein. For transcription to reach significant levels both HngR and banana must be present
mutable notes: Monkeys can be dangerous when aggrevated
mutable provenance: Curious George's very own DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J16101_sequence/1

pspoIIE
type: ComponentDefinition
persistent identity: BBa J01005
display id: BBa_J01005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pspoIIE promoter (spo0A J01004, positive)
creator: Golden Bear
created: 2005-10-31T12:00:00Z
modified: 2015-08-31T04:08:11Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _13_
m user id: 0
owner id: 395
owning group id: 13
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: promoter from b. subtilis, activated by spo0A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J01005_sequence/1

BBa_K259007
type: ComponentDefinition
persistent identity: BBa K259007
display id: BBa_K259007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AraC Promoter fused with RBS
creator: Petros Mina
created: 2009-09-08T11:00:00Z
modified: 2015-05-08T01:11:42Z
discontinued: false
dominant: false
group u list: _357_
m user id: 0
owner id: 5318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part has the arabinose regulated promoter (AraC/pBAD) upstream of a ribosome binding site. This is ideal for inserting a protein to be expressed downstream of this part as it's expression can be regulated by the presence of the concentration of arabinose in the growing environment/media of the cells. ==What this part actually does== This composite part includes the AraC/pBAD promoter and a strong ribosome binding site designed from the Anderson family of RBS. The AraC/pBAD promoter can be used with prokaryotic chassis to regulate expresion of proteins. When the AraC/pBAD promoter is placed upstream of your canditate translational unit (protein to be expressed) it allows control of the trancription of the said protein. This is done via controlling the concentration of arabinose sugar in the environment/solution of bacterial growth. Controlling the transcription will not ensure translation. The ribosome binding site is ideally placed a small distance away from the promoter site to ensure that the ribosome will bind and start translating the protein coding sequence found downstream.
mutable notes: The part was designed with RFC10 assembly from supplied biobricks of the iGEM2009 distribution.
mutable provenance: The AraC promoter can be found naturally at 1.52min of the E.coli genome ([http://ecogene.org/geneinfo.php?eg_id=EG10054 EcoGene number EG10054]. The RBS was taken from the designed sequences of RBS by the Anderson family. For more information visit the original part page [http://partsregistry.org/Part:BBa_J61100 here].
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_R0080
component: BBa_J61100
component: BBa_R0081
sequence annotation: BBa_R0081
sequence annotation: BBa_R0080
sequence annotation: BBa_J61100
sequence: https://synbiohub.org/public/igem/BBa_K259007_sequence/1

BBa_R0080
type: Component
persistent identity: component2020546
display id: component2020546
version: 1
top level: BBa_K259007
access: public
definition: AraC

BBa_J61100
type: Component
persistent identity: component2020547
display id: component2020547
version: 1
top level: BBa_K259007
access: public
definition: https://synbiohub.org/public/igem/BBa_J61100/1

BBa_R0081
type: Component
persistent identity: component2020539
display id: component2020539
version: 1
top level: BBa_K259007
access: public
definition: https://synbiohub.org/public/igem/BBa_R0081/1

BBa_R0081
type: SequenceAnnotation
persistent identity: annotation2020539
display id: annotation2020539
version: 1
top level: BBa_K259007
location: https://synbiohub.org/public/igem/BBa_K259007/annotation2020539/range2020539/1
component: BBa_R0081

https://synbiohub.org/public/igem/BBa_K259007/annotation2020539/range2020539/1
type: Range
persistent identity: range2020539
display id: range2020539
version: 1
direction: direction/reverse
top level: BBa_K259007
start: 1
end: 183
orientation: inline

BBa_R0080
type: SequenceAnnotation
persistent identity: annotation2020546
display id: annotation2020546
version: 1
top level: BBa_K259007
location: https://synbiohub.org/public/igem/BBa_K259007/annotation2020546/range2020546/1
component: BBa_R0080

https://synbiohub.org/public/igem/BBa_K259007/annotation2020546/range2020546/1
type: Range
persistent identity: range2020546
display id: range2020546
version: 1
direction: direction/reverse
top level: BBa_K259007
start: 192
end: 340
orientation: inline

BBa_J61100
type: SequenceAnnotation
persistent identity: annotation2020547
display id: annotation2020547
version: 1
top level: BBa_K259007
location: https://synbiohub.org/public/igem/BBa_K259007/annotation2020547/range2020547/1
component: BBa_J61100

https://synbiohub.org/public/igem/BBa_K259007/annotation2020547/range2020547/1
type: Range
persistent identity: range2020547
display id: range2020547
version: 1
direction: direction/reverse
top level: BBa_K259007
start: 349
end: 360
orientation: inline

BBa_J64000
type: ComponentDefinition
persistent identity: BBa J64000
display id: BBa_J64000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rhlI promoter
creator: Jeffrey J Tabor
created: 2007-01-15T12:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 88
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Promoter driving the rhlI gene in Pseudomonas aeruginosa. Activated by the RhlR+C4HSL. Also likely activated by LasR+3OC12HSL
mutable notes: none
mutable provenance: Genomic sequence of Pseudomonas aeruginosa
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Rhl box
sequence: https://synbiohub.org/public/igem/BBa_J64000_sequence/1

Rhl box
type: SequenceAnnotation
persistent identity: annotation1910614
display id: annotation1910614
version: 1
top level: BBa_J64000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J64000/annotation1910614/range1910614/1

https://synbiohub.org/public/igem/BBa_J64000/annotation1910614/range1910614/1
type: Range
persistent identity: range1910614
display id: range1910614
version: 1
direction: direction/forward
top level: BBa_J64000
start: 11
end: 30
orientation: inline

lux pR
type: ComponentDefinition
persistent identity: BBa R0062
display id: BBa_R0062
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (luxR & HSL regulated -- lux pR)
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Promoter activated by LuxR in concert with HSL The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the LuxR activator protein complexed with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
mutable notes: This promoter is based on the Vibrio fischeri quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below. <img src="<bb_file>Image1.gif</bb_file>" width="614" height="362">
mutable provenance: V. fischeri
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: BBa_R0062
sequence annotation: -35
sequence annotation: start
sequence annotation: LuxR/HSL
sequence: https://synbiohub.org/public/igem/BBa_R0062_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation2047
display id: annotation2047
version: 1
top level: lux pR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2047/range2047/1

https://synbiohub.org/public/igem/BBa_R0062/annotation2047/range2047/1
type: Range
persistent identity: range2047
display id: range2047
version: 1
direction: direction/forward
top level: lux pR
start: 42
end: 47
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation7070
display id: annotation7070
version: 1
top level: lux pR
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0062/annotation7070/range7070/1

https://synbiohub.org/public/igem/BBa_R0062/annotation7070/range7070/1
type: Range
persistent identity: range7070
display id: range7070
version: 1
direction: direction/forward
top level: lux pR
start: 1
end: 55
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2046
display id: annotation2046
version: 1
top level: lux pR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2046/range2046/1

https://synbiohub.org/public/igem/BBa_R0062/annotation2046/range2046/1
type: Range
persistent identity: range2046
display id: range2046
version: 1
direction: direction/forward
top level: lux pR
start: 20
end: 25
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2048
display id: annotation2048
version: 1
top level: lux pR
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2048/range2048/1

https://synbiohub.org/public/igem/BBa_R0062/annotation2048/range2048/1
type: Range
persistent identity: range2048
display id: range2048
version: 1
direction: direction/forward
top level: lux pR
start: 53
end: 53
orientation: inline

LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2045
display id: annotation2045
version: 1
top level: lux pR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2045/range2045/1

https://synbiohub.org/public/igem/BBa_R0062/annotation2045/range2045/1
type: Range
persistent identity: range2045
display id: range2045
version: 1
direction: direction/forward
top level: lux pR
start: 1
end: 20
orientation: inline

pBAD:Cpn10
type: ComponentDefinition
persistent identity: BBa K538300
display id: BBa_K538300
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBAD + RBS + Cpn10
creator: Bas Stringer
created: 2011-09-07T11:00:00Z
modified: 2015-05-08T01:12:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _704_
m user id: 0
owner id: 9985
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K538000
component: BBa_B0034
component: BBa_K206000
sequence annotation: BBa_K206000
sequence annotation: BBa_B0034
sequence annotation: BBa_K538000
sequence: https://synbiohub.org/public/igem/BBa_K538300_sequence/1

BBa_K538000
type: Component
persistent identity: component2126764
display id: component2126764
version: 1
top level: pBAD:Cpn10
access: public
definition: https://synbiohub.org/public/igem/BBa_K538000/1

BBa_B0034
type: Component
persistent identity: component2126761
display id: component2126761
version: 1
top level: pBAD:Cpn10
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K206000
type: Component
persistent identity: component2126759
display id: component2126759
version: 1
top level: pBAD:Cpn10
access: public
definition: pBAD

BBa_K206000
type: SequenceAnnotation
persistent identity: annotation2126759
display id: annotation2126759
version: 1
top level: pBAD:Cpn10
location: https://synbiohub.org/public/igem/BBa_K538300/annotation2126759/range2126759/1
component: BBa_K206000

https://synbiohub.org/public/igem/BBa_K538300/annotation2126759/range2126759/1
type: Range
persistent identity: range2126759
display id: range2126759
version: 1
direction: direction/reverse
top level: pBAD:Cpn10
start: 1
end: 130
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2126761
display id: annotation2126761
version: 1
top level: pBAD:Cpn10
location: https://synbiohub.org/public/igem/BBa_K538300/annotation2126761/range2126761/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K538300/annotation2126761/range2126761/1
type: Range
persistent identity: range2126761
display id: range2126761
version: 1
direction: direction/reverse
top level: pBAD:Cpn10
start: 139
end: 150
orientation: inline

BBa_K538000
type: SequenceAnnotation
persistent identity: annotation2126764
display id: annotation2126764
version: 1
top level: pBAD:Cpn10
location: https://synbiohub.org/public/igem/BBa_K538300/annotation2126764/range2126764/1
component: BBa_K538000

https://synbiohub.org/public/igem/BBa_K538300/annotation2126764/range2126764/1
type: Range
persistent identity: range2126764
display id: range2126764
version: 1
direction: direction/reverse
top level: pBAD:Cpn10
start: 157
end: 453
orientation: inline

BBa_I751501
type: ComponentDefinition
persistent identity: BBa I751501
display id: BBa_I751501
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: plux-cI hybrid promoter
creator: Kenichiro Iwasaki
created: 2007-10-24T11:00:00Z
modified: 2015-08-31T04:08:06Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _151_
m user id: 0
owner id: 2188
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: sense TWO INPUTS, activation by AHL and repression by LacI.
mutable notes: Based on the AHL regulated BioBrick part R0062 and the previous work on LacI regulated promoter (ref. 1), we designed a new promoter regulated by AHL and LacI. Since this promoter is a kind of a "HYBRID" of the two, we call it HYBRID PROMOTER. Compared with existing BioBrick hybrid promoter R0065, this promoter is different in that its sequence is much shorter with lux box and -35 region overlapping, and that it is repressed by LacI, not cI. Also, the output is clearly detected as shown in Fig. 3.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: plux-cI hybrid promoter
sequence annotation: -35
sequence annotation: OR2
sequence annotation: OR1
sequence annotation: -10
sequence annotation: luxR binding
sequence: https://synbiohub.org/public/igem/BBa_I751501_sequence/1

plux-cI hybrid promoter
type: SequenceAnnotation
persistent identity: annotation1955478
display id: annotation1955478
version: 1
top level: BBa_I751501
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I751501/annotation1955478/range1955478/1

https://synbiohub.org/public/igem/BBa_I751501/annotation1955478/range1955478/1
type: Range
persistent identity: range1955478
display id: range1955478
version: 1
direction: direction/forward
top level: BBa_I751501
start: 19
end: 66
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1955479
display id: annotation1955479
version: 1
top level: BBa_I751501
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I751501/annotation1955479/range1955479/1

https://synbiohub.org/public/igem/BBa_I751501/annotation1955479/range1955479/1
type: Range
persistent identity: range1955479
display id: range1955479
version: 1
direction: direction/forward
top level: BBa_I751501
start: 19
end: 25
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation1955481
display id: annotation1955481
version: 1
top level: BBa_I751501
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I751501/annotation1955481/range1955481/1

https://synbiohub.org/public/igem/BBa_I751501/annotation1955481/range1955481/1
type: Range
persistent identity: range1955481
display id: range1955481
version: 1
direction: direction/forward
top level: BBa_I751501
start: 26
end: 41
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation1955477
display id: annotation1955477
version: 1
top level: BBa_I751501
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I751501/annotation1955477/range1955477/1

https://synbiohub.org/public/igem/BBa_I751501/annotation1955477/range1955477/1
type: Range
persistent identity: range1955477
display id: range1955477
version: 1
direction: direction/forward
top level: BBa_I751501
start: 48
end: 66
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1955480
display id: annotation1955480
version: 1
top level: BBa_I751501
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I751501/annotation1955480/range1955480/1

https://synbiohub.org/public/igem/BBa_I751501/annotation1955480/range1955480/1
type: Range
persistent identity: range1955480
display id: range1955480
version: 1
direction: direction/forward
top level: BBa_I751501
start: 42
end: 47
orientation: inline

luxR binding
type: SequenceAnnotation
persistent identity: annotation1955482
display id: annotation1955482
version: 1
top level: BBa_I751501
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I751501/annotation1955482/range1955482/1

https://synbiohub.org/public/igem/BBa_I751501/annotation1955482/range1955482/1
type: Range
persistent identity: range1955482
display id: range1955482
version: 1
direction: direction/forward
top level: BBa_I751501
start: 1
end: 18
orientation: inline

BBa_K117002
type: ComponentDefinition
persistent identity: BBa K117002
display id: BBa_K117002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LsrA promoter (indirectly activated by AI-2)
creator: Nguyen Xuan Hung
created: 2008-10-06T11:00:00Z
modified: 2015-05-08T01:09:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _209_
m user id: 0
owner id: 2774
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: The regulatory network for the uptake of Escherichia coli autoinducer 2 (AI-2) is comprised of a transporter complex, LsrABCD; its repressor, LsrR; and a cognate signal kinase, LsrK. This network is an integral part of the AI-2 quorum-sensing (QS) system. Once uptaken into the cell, AI-2 will be phosphorylated under the effect of LsrK. Without the presence of AI-2, LsrA promoter is inhibited by the repressor, LsrR. Phosphorylated AI-2 will prevent LsrR from inhibiting this promoter, hence activating it so that the downstream sequence can be expressed. To prevent the cell from self-inducing this promoter, we must suppress its AI-2 producing capability. It can be done by knocking out LuxS, one of the vital gene required for producing AI-2. By doing that, LsrA promoter can only be activated under the presence of AI-2 from other sources than the host cell which is LuxS mutant (say, by contact with normal cells). More information on AI-2 quorum-sensing (QS) system please refer to: http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1952038
mutable notes: to be updated
mutable provenance: to be updated
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: RBS BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K117002_sequence/1

RBS BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1979372
display id: annotation1979372
version: 1
top level: BBa_K117002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K117002/annotation1979372/range1979372/1

https://synbiohub.org/public/igem/BBa_K117002/annotation1979372/range1979372/1
type: Range
persistent identity: range1979372
display id: range1979372
version: 1
direction: direction/forward
top level: BBa_K117002
start: 112
end: 123
orientation: inline

BBa_K731201
type: ComponentDefinition
persistent identity: BBa K731201
display id: BBa_K731201
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Arabinose inducible araC-pBAD promoter
creator: Daniele Rossetto
created: 2012-08-02T11:00:00Z
modified: 2015-05-08T01:13:06Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _977_
m user id: 0
owner id: 11997
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: This E. coli promoter is positively regulated by arabinose. araC is the regulator protein, binding to arabinose prevents the repression of pBAD.
mutable notes: This part is similar to BBa_I0500, but it differs from that one in several point mutations in the sequence.
mutable provenance: E. coli genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: pBAD
sequence annotation: araC
sequence annotation: PC
sequence: https://synbiohub.org/public/igem/BBa_K731201_sequence/1

pBAD
type: SequenceAnnotation
persistent identity: annotation2179311
display id: annotation2179311
version: 1
top level: BBa_K731201
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K731201/annotation2179311/range2179311/1

https://synbiohub.org/public/igem/BBa_K731201/annotation2179311/range2179311/1
type: Range
persistent identity: range2179311
display id: range2179311
version: 1
direction: direction/forward
top level: BBa_K731201
start: 1080
end: 1203
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation2179313
display id: annotation2179313
version: 1
top level: BBa_K731201
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K731201/annotation2179313/range2179313/1

https://synbiohub.org/public/igem/BBa_K731201/annotation2179313/range2179313/1
type: Range
persistent identity: range2179313
display id: range2179313
version: 1
direction: direction/reverse
top level: BBa_K731201
start: 1
end: 1080
orientation: inline

PC
type: SequenceAnnotation
persistent identity: annotation2179312
display id: annotation2179312
version: 1
top level: BBa_K731201
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K731201/annotation2179312/range2179312/1

https://synbiohub.org/public/igem/BBa_K731201/annotation2179312/range2179312/1
type: Range
persistent identity: range2179312
display id: range2179312
version: 1
direction: direction/reverse
top level: BBa_K731201
start: 1043
end: 1114
orientation: inline

lac Or2-62
type: ComponentDefinition
persistent identity: BBa I12210
display id: BBa_I12210
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: plac Or2-62 (positive)
creator: rwald
created: 2004-08-06T11:00:00Z
modified: 2015-08-31T04:07:31Z
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 120
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is a modification of the standard plac operon with everything before the -35 site removed, the Or2 site from the lambda promoter inserted at the beginning, and a 17-bp spacer added between the two.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: cI OR2 (lambda)
sequence annotation: -10
sequence annotation: spacer
sequence: https://synbiohub.org/public/igem/BBa_I12210_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation1031725
display id: annotation1031725
version: 1
top level: lac Or2-62
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I12210/annotation1031725/range1031725/1

https://synbiohub.org/public/igem/BBa_I12210/annotation1031725/range1031725/1
type: Range
persistent identity: range1031725
display id: range1031725
version: 1
direction: direction/forward
top level: lac Or2-62
start: 35
end: 40
orientation: inline

cI OR2 (lambda)
type: SequenceAnnotation
persistent identity: annotation1031724
display id: annotation1031724
version: 1
top level: lac Or2-62
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12210/annotation1031724/range1031724/1

https://synbiohub.org/public/igem/BBa_I12210/annotation1031724/range1031724/1
type: Range
persistent identity: range1031724
display id: range1031724
version: 1
direction: direction/forward
top level: lac Or2-62
start: 1
end: 17
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1031726
display id: annotation1031726
version: 1
top level: lac Or2-62
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I12210/annotation1031726/range1031726/1

https://synbiohub.org/public/igem/BBa_I12210/annotation1031726/range1031726/1
type: Range
persistent identity: range1031726
display id: range1031726
version: 1
direction: direction/forward
top level: lac Or2-62
start: 59
end: 65
orientation: inline

spacer
type: SequenceAnnotation
persistent identity: annotation1031727
display id: annotation1031727
version: 1
top level: lac Or2-62
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I12210/annotation1031727/range1031727/1

https://synbiohub.org/public/igem/BBa_I12210/annotation1031727/range1031727/1
type: Range
persistent identity: range1031727
display id: range1031727
version: 1
direction: direction/forward
top level: lac Or2-62
start: 18
end: 34
orientation: inline

hybB
type: ComponentDefinition
persistent identity: BBa J45503
display id: BBa_J45503
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: hybB Cold Shock Promoter
creator: Stephen Payne
created: 2006-06-20T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: false
experience: experience/None
group u list: _84_
m user id: 0
owner id: 642
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This cold shock promoter is only active at temperatures lower than 30 degree Celsius.
mutable notes: None.
mutable provenance: Chris Voigt Lab at UCSF
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J45503_sequence/1

BBa_K1486050
type: ComponentDefinition
persistent identity: BBa K1486050
display id: BBa_K1486050
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CpxR promoter RV + RFP
creator: EPFL iGem team 2014
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: to edit
mutable notes: to edit
mutable provenance: to edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K1486047
component: BBa_I13507
sequence annotation: BBa_I13507
sequence annotation: BBa_K1486047
sequence: https://synbiohub.org/public/igem/BBa_K1486050_sequence/1

BBa_K1486047
type: Component
persistent identity: component2398450
display id: component2398450
version: 1
top level: BBa_K1486050
access: public
definition: BBa_K1486047

BBa_I13507
type: Component
persistent identity: component2398462
display id: component2398462
version: 1
top level: BBa_K1486050
access: public
definition: https://synbiohub.org/public/igem/BBa_I13507/1

BBa_I13507
type: SequenceAnnotation
persistent identity: annotation2398462
display id: annotation2398462
version: 1
top level: BBa_K1486050
location: https://synbiohub.org/public/igem/BBa_K1486050/annotation2398462/range2398462/1
component: BBa_I13507

https://synbiohub.org/public/igem/BBa_K1486050/annotation2398462/range2398462/1
type: Range
persistent identity: range2398462
display id: range2398462
version: 1
direction: direction/reverse
top level: BBa_K1486050
start: 147
end: 1007
orientation: inline

BBa_K1486047
type: SequenceAnnotation
persistent identity: annotation2398450
display id: annotation2398450
version: 1
top level: BBa_K1486050
location: https://synbiohub.org/public/igem/BBa_K1486050/annotation2398450/range2398450/1
component: BBa_K1486047

https://synbiohub.org/public/igem/BBa_K1486050/annotation2398450/range2398450/1
type: Range
persistent identity: range2398450
display id: range2398450
version: 1
direction: direction/reverse
top level: BBa_K1486050
start: 1
end: 146
orientation: inline

HSP
type: ComponentDefinition
persistent identity: BBa K338001
display id: BBa_K338001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Heat Shock Promoter (HSP)
creator: 2010 Caltech iGEM Team
created: 2010-10-23T11:00:00Z
modified: 2015-05-08T01:12:07Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _454_
m user id: 0
owner id: 6189
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is a modified K112400 heat shock promoter in BBa standard.
mutable notes: None
mutable provenance: Modified BBa_K112400
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K338001_sequence/1

BBa_K112900
type: ComponentDefinition
persistent identity: BBa K112900
display id: BBa_K112900
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pbad
creator: Bing Xia
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:19Z
discontinued: false
dominant: false
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Arabinose induced promoter. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K112900_sequence/1

BBa_K864400
type: ComponentDefinition
persistent identity: BBa K864400
display id: BBa_K864400
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ptac, trp & lac regulated promoter
creator: Erik Lundin
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The Ptac promoter is a functional hybrid promoter, derived from the trp and lac promoters, that are regulated by trp and lac [1]. This part also exist together with lacI, part BBa_K180000 [1] Proc. Natl. Acad. Sci. USA, Vol. 80, pp. 21-25
mutable notes: Oligos ordered from SigmaAldrich were annealed to form a double stranded DNA segment, ready to be ligated into any BioBrick backbone.
mutable provenance: Created from oligos ordered from SigmaAldrich. Oligo sequences: Ptac+ AATTCGCGGCCGCTTCTAGAGGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTA Ptac- CTAGTAAATTGTTATCCGCTCACAATTCCACACATTATACGAGCCGATGATTAATTGTCAACAGCTCCTCTAGAAGCGGCCGCG
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Ptac
sequence annotation: lacO1 site
sequence annotation: -10
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_K864400_sequence/1

Ptac
type: SequenceAnnotation
persistent identity: annotation2197236
display id: annotation2197236
version: 1
top level: BBa_K864400
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K864400/annotation2197236/range2197236/1

https://synbiohub.org/public/igem/BBa_K864400/annotation2197236/range2197236/1
type: Range
persistent identity: range2197236
display id: range2197236
version: 1
direction: direction/forward
top level: BBa_K864400
start: 1
end: 40
orientation: inline

lacO1 site
type: SequenceAnnotation
persistent identity: annotation2197240
display id: annotation2197240
version: 1
top level: BBa_K864400
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K864400/annotation2197240/range2197240/1

https://synbiohub.org/public/igem/BBa_K864400/annotation2197240/range2197240/1
type: Range
persistent identity: range2197240
display id: range2197240
version: 1
direction: direction/forward
top level: BBa_K864400
start: 36
end: 61
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2197238
display id: annotation2197238
version: 1
top level: BBa_K864400
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K864400/annotation2197238/range2197238/1

https://synbiohub.org/public/igem/BBa_K864400/annotation2197238/range2197238/1
type: Range
persistent identity: range2197238
display id: range2197238
version: 1
direction: direction/forward
top level: BBa_K864400
start: 29
end: 33
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2197237
display id: annotation2197237
version: 1
top level: BBa_K864400
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K864400/annotation2197237/range2197237/1

https://synbiohub.org/public/igem/BBa_K864400/annotation2197237/range2197237/1
type: Range
persistent identity: range2197237
display id: range2197237
version: 1
direction: direction/forward
top level: BBa_K864400
start: 7
end: 12
orientation: inline

BBa_K110006
type: ComponentDefinition
persistent identity: BBa K110006
display id: BBa_K110006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Alpha-Cell Promoter MF(ALPHA)1
creator: James DiCarlo
created: 2008-05-29T11:00:00Z
modified: 2015-05-08T01:09:08Z
discontinued: false
dominant: false
experience: experience/None
group u list: _201_
m user id: 0
owner id: 2669
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This promoter was isolated by taking 500bp upstream of the MF(ALPHA)1 gene of S. cerevisiae. This promoter will be activated when alpha factor is expressed in an alpha haploid yeast cell.
mutable notes: This gene is on the Watson strand of the entered sequence.
mutable provenance: SGD
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: MCM1
sequence annotation: MF(alpha)1
sequence: https://synbiohub.org/public/igem/BBa_K110006_sequence/1

MCM1
type: SequenceAnnotation
persistent identity: annotation1997688
display id: annotation1997688
version: 1
top level: BBa_K110006
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110006/annotation1997688/range1997688/1

https://synbiohub.org/public/igem/BBa_K110006/annotation1997688/range1997688/1
type: Range
persistent identity: range1997688
display id: range1997688
version: 1
direction: direction/forward
top level: BBa_K110006
start: 19
end: 34
orientation: inline

MCM1
type: SequenceAnnotation
persistent identity: annotation1997689
display id: annotation1997689
version: 1
top level: BBa_K110006
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110006/annotation1997689/range1997689/1

https://synbiohub.org/public/igem/BBa_K110006/annotation1997689/range1997689/1
type: Range
persistent identity: range1997689
display id: range1997689
version: 1
direction: direction/forward
top level: BBa_K110006
start: 136
end: 161
orientation: inline

MCM1
type: SequenceAnnotation
persistent identity: annotation1997690
display id: annotation1997690
version: 1
top level: BBa_K110006
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110006/annotation1997690/range1997690/1

https://synbiohub.org/public/igem/BBa_K110006/annotation1997690/range1997690/1
type: Range
persistent identity: range1997690
display id: range1997690
version: 1
direction: direction/forward
top level: BBa_K110006
start: 92
end: 102
orientation: inline

MF(alpha)1
type: SequenceAnnotation
persistent identity: annotation1997687
display id: annotation1997687
version: 1
top level: BBa_K110006
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K110006/annotation1997687/range1997687/1

https://synbiohub.org/public/igem/BBa_K110006/annotation1997687/range1997687/1
type: Range
persistent identity: range1997687
display id: range1997687
version: 1
direction: direction/forward
top level: BBa_K110006
start: 1
end: 501
orientation: inline

cinR
type: ComponentDefinition
persistent identity: BBa R0078
display id: BBa_R0078
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (cinR and HSL regulated)
creator: Drew Endy
created: 2004-01-29T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable provenance: Rhizobium leguminosarum
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: unidentified/uncharacterized CinR dependent
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_R0078_sequence/1

unidentified/uncharacterized CinR dependent
type: SequenceAnnotation
persistent identity: annotation318234
display id: annotation318234
version: 1
top level: cinR
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_R0078/annotation318234/range318234/1

https://synbiohub.org/public/igem/BBa_R0078/annotation318234/range318234/1
type: Range
persistent identity: range318234
display id: range318234
version: 1
direction: direction/forward
top level: cinR
start: 1
end: 220
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation318244
display id: annotation318244
version: 1
top level: cinR
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_R0078/annotation318244/range318244/1

https://synbiohub.org/public/igem/BBa_R0078/annotation318244/range318244/1
type: Range
persistent identity: range318244
display id: range318244
version: 1
direction: direction/forward
top level: cinR
start: 35
end: 74
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation318250
display id: annotation318250
version: 1
top level: cinR
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_R0078/annotation318250/range318250/1

https://synbiohub.org/public/igem/BBa_R0078/annotation318250/range318250/1
type: Range
persistent identity: range318250
display id: range318250
version: 1
direction: direction/forward
top level: cinR
start: 91
end: 136
orientation: inline

BBa_K2110006
type: ComponentDefinition
persistent identity: BBa K2110006
display id: BBa_K2110006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TPA regulatory gene expression system
creator: Le He
created: 2016-10-05T11:00:00Z
modified: 2016-10-06T01:20:49Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2578_
m user id: 32957
owner id: 32957
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part consists of the enhanced promoter PGK1(BBa_K2110012), the tpaR gene(BBa_K2110002), and the terminator CYC1（BBa_K2110000）. The tpaR gene encodes the terephthalic acid (TPA) positive feedback regulatory protein. This protein can bind the TPA inducible leader sequence (TILS) at the existence of TPA and induce the expression of downstream gene. The tpaR protein belongs to the IclR protein family and has helix-turn-helix domain.
mutable notes: /
mutable provenance: BBa_K2110012 BBa_K2110002 BBa_K2110000
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K2110012
component: BBa_K2110002
component: BBa_K2110000
sequence annotation: BBa_K2110012
sequence annotation: BBa_K2110000
sequence annotation: BBa_K2110002
sequence: https://synbiohub.org/public/igem/BBa_K2110006_sequence/1

BBa_K2110012
type: Component
persistent identity: component2486540
display id: component2486540
version: 1
top level: BBa_K2110006
access: public
definition: https://synbiohub.org/public/igem/BBa_K2110012/1

BBa_K2110002
type: Component
persistent identity: component2486542
display id: component2486542
version: 1
top level: BBa_K2110006
access: public
definition: BBa_K2110002

BBa_K2110000
type: Component
persistent identity: component2486543
display id: component2486543
version: 1
top level: BBa_K2110006
access: public
definition: https://synbiohub.org/public/igem/BBa_K2110000/1

BBa_K2110012
type: SequenceAnnotation
persistent identity: annotation2486540
display id: annotation2486540
version: 1
top level: BBa_K2110006
location: https://synbiohub.org/public/igem/BBa_K2110006/annotation2486540/range2486540/1
component: BBa_K2110012

https://synbiohub.org/public/igem/BBa_K2110006/annotation2486540/range2486540/1
type: Range
persistent identity: range2486540
display id: range2486540
version: 1
direction: direction/reverse
top level: BBa_K2110006
start: 1
end: 781
orientation: inline

BBa_K2110000
type: SequenceAnnotation
persistent identity: annotation2486543
display id: annotation2486543
version: 1
top level: BBa_K2110006
location: https://synbiohub.org/public/igem/BBa_K2110006/annotation2486543/range2486543/1
component: BBa_K2110000

https://synbiohub.org/public/igem/BBa_K2110006/annotation2486543/range2486543/1
type: Range
persistent identity: range2486543
display id: range2486543
version: 1
direction: direction/reverse
top level: BBa_K2110006
start: 1603
end: 1850
orientation: inline

BBa_K2110002
type: SequenceAnnotation
persistent identity: annotation2486542
display id: annotation2486542
version: 1
top level: BBa_K2110006
location: https://synbiohub.org/public/igem/BBa_K2110006/annotation2486542/range2486542/1
component: BBa_K2110002

https://synbiohub.org/public/igem/BBa_K2110006/annotation2486542/range2486542/1
type: Range
persistent identity: range2486542
display id: range2486542
version: 1
direction: direction/reverse
top level: BBa_K2110006
start: 788
end: 1594
orientation: inline

BBa_K091143
type: ComponentDefinition
persistent identity: BBa K091143
display id: BBa_K091143
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLas/cI Hybrid Promoter
creator: Xiao Zhu
created: 2008-07-14T11:00:00Z
modified: 2015-05-08T01:08:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _191_
m user id: 0
owner id: 3130
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: this hybrid promoter is designed to be consecutively off.It combines with plasR(R0079) and lambda cI OR1(see C0051).Adding P-AI-1 sould activate the promoter, while repressed by lambda cI.
mutable notes: "CI binding of the OR region. It binds OR1 with highest affinity, OR2 with next highest, and OR3 with lowest"(http://www.nonanone.com/qw/files/Biology%204450/test2_lecture8,9.txt)
mutable provenance: .
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: cI OR1
sequence annotation: plasR OP2
sequence annotation: -35
sequence annotation: -10
sequence annotation: plasR OP1
sequence: https://synbiohub.org/public/igem/BBa_K091143_sequence/1

cI OR1
type: SequenceAnnotation
persistent identity: annotation1967106
display id: annotation1967106
version: 1
top level: BBa_K091143
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K091143/annotation1967106/range1967106/1

https://synbiohub.org/public/igem/BBa_K091143/annotation1967106/range1967106/1
type: Range
persistent identity: range1967106
display id: range1967106
version: 1
direction: direction/forward
top level: BBa_K091143
start: 147
end: 164
orientation: inline

plasR OP2
type: SequenceAnnotation
persistent identity: annotation1967108
display id: annotation1967108
version: 1
top level: BBa_K091143
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K091143/annotation1967108/range1967108/1

https://synbiohub.org/public/igem/BBa_K091143/annotation1967108/range1967108/1
type: Range
persistent identity: range1967108
display id: range1967108
version: 1
direction: direction/forward
top level: BBa_K091143
start: 106
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1967105
display id: annotation1967105
version: 1
top level: BBa_K091143
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K091143/annotation1967105/range1967105/1

https://synbiohub.org/public/igem/BBa_K091143/annotation1967105/range1967105/1
type: Range
persistent identity: range1967105
display id: range1967105
version: 1
direction: direction/forward
top level: BBa_K091143
start: 117
end: 122
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1967104
display id: annotation1967104
version: 1
top level: BBa_K091143
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K091143/annotation1967104/range1967104/1

https://synbiohub.org/public/igem/BBa_K091143/annotation1967104/range1967104/1
type: Range
persistent identity: range1967104
display id: range1967104
version: 1
direction: direction/forward
top level: BBa_K091143
start: 140
end: 145
orientation: inline

plasR OP1
type: SequenceAnnotation
persistent identity: annotation1967107
display id: annotation1967107
version: 1
top level: BBa_K091143
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K091143/annotation1967107/range1967107/1

https://synbiohub.org/public/igem/BBa_K091143/annotation1967107/range1967107/1
type: Range
persistent identity: range1967107
display id: range1967107
version: 1
direction: direction/forward
top level: BBa_K091143
start: 46
end: 63
orientation: inline

BBa_K1709006
type: ComponentDefinition
persistent identity: BBa K1709006
display id: BBa_K1709006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS-LuxI-His-RBS-LuxR-E-Term-Plux
creator: Astrid Deryckere
created: 2015-09-17T11:00:00Z
modified: 2015-09-18T11:29:08Z
discontinued: false
dominant: false
group u list: _2129_
m user id: 25389
owner id: 25389
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Autoinducer synthetase for acyl-homoserine lactones (AHL): LuxI synthesizes AHL (3OC6HSL). Two molecules of AHL form a complex with two molecules of the LuxR protein. This complex then binds to a palyndromic site on the lux promoter, increasing its transcription rate. LuxI, LuxR and the right lux promoter are all derived from V. fischeri. In absence of AHL, the right Lux promotor only gives weak constitutive expression of downstream genes. Any gene you would like to be controlled by AHL and thus cell density can be cloned after this complex.
mutable notes: For the ease of cloning, the sequence was devided in multiple gBlocks. Therefor, there is a mistake in the composite part sequence since the scar is only partly present.
mutable provenance: The Lux operon originates from V. Fischeri.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K1709001
component: BBa_B1002
component: BBa_J61100
component: BBa_R0062
component: BBa_K1709004
sequence annotation: BBa_K1709004
sequence annotation: BBa_K1709001
sequence annotation: BBa_R0062
sequence annotation: BBa_B1002
sequence annotation: BBa_J61100
sequence: https://synbiohub.org/public/igem/BBa_K1709006_sequence/1

BBa_K1709001
type: Component
persistent identity: component2473330
display id: component2473330
version: 1
top level: BBa_K1709006
access: public
definition: https://synbiohub.org/public/igem/BBa_K1709001/1

BBa_B1002
type: Component
persistent identity: component2473341
display id: component2473341
version: 1
top level: BBa_K1709006
access: public
definition: https://synbiohub.org/public/igem/BBa_B1002/1

BBa_J61100
type: Component
persistent identity: component2473331
display id: component2473331
version: 1
top level: BBa_K1709006
access: public
definition: https://synbiohub.org/public/igem/BBa_J61100/1

BBa_R0062
type: Component
persistent identity: component2473343
display id: component2473343
version: 1
top level: BBa_K1709006
access: public
definition: lux pR

BBa_K1709004
type: Component
persistent identity: component2473336
display id: component2473336
version: 1
top level: BBa_K1709006
access: public
definition: BBa_K1709004

BBa_K1709004
type: SequenceAnnotation
persistent identity: annotation2473336
display id: annotation2473336
version: 1
top level: BBa_K1709006
location: https://synbiohub.org/public/igem/BBa_K1709006/annotation2473336/range2473336/1
component: BBa_K1709004

https://synbiohub.org/public/igem/BBa_K1709006/annotation2473336/range2473336/1
type: Range
persistent identity: range2473336
display id: range2473336
version: 1
direction: direction/reverse
top level: BBa_K1709006
start: 654
end: 1454
orientation: inline

BBa_K1709001
type: SequenceAnnotation
persistent identity: annotation2473330
display id: annotation2473330
version: 1
top level: BBa_K1709006
location: https://synbiohub.org/public/igem/BBa_K1709006/annotation2473330/range2473330/1
component: BBa_K1709001

https://synbiohub.org/public/igem/BBa_K1709006/annotation2473330/range2473330/1
type: Range
persistent identity: range2473330
display id: range2473330
version: 1
direction: direction/reverse
top level: BBa_K1709006
start: 1
end: 627
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2473343
display id: annotation2473343
version: 1
top level: BBa_K1709006
location: https://synbiohub.org/public/igem/BBa_K1709006/annotation2473343/range2473343/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K1709006/annotation2473343/range2473343/1
type: Range
persistent identity: range2473343
display id: range2473343
version: 1
direction: direction/forward
top level: BBa_K1709006
start: 1505
end: 1559
orientation: inline

BBa_B1002
type: SequenceAnnotation
persistent identity: annotation2473341
display id: annotation2473341
version: 1
top level: BBa_K1709006
location: https://synbiohub.org/public/igem/BBa_K1709006/annotation2473341/range2473341/1
component: BBa_B1002

https://synbiohub.org/public/igem/BBa_K1709006/annotation2473341/range2473341/1
type: Range
persistent identity: range2473341
display id: range2473341
version: 1
direction: direction/reverse
top level: BBa_K1709006
start: 1463
end: 1496
orientation: inline

BBa_J61100
type: SequenceAnnotation
persistent identity: annotation2473331
display id: annotation2473331
version: 1
top level: BBa_K1709006
location: https://synbiohub.org/public/igem/BBa_K1709006/annotation2473331/range2473331/1
component: BBa_J61100

https://synbiohub.org/public/igem/BBa_K1709006/annotation2473331/range2473331/1
type: Range
persistent identity: range2473331
display id: range2473331
version: 1
direction: direction/reverse
top level: BBa_K1709006
start: 636
end: 647
orientation: inline

pBAD:CspA
type: ComponentDefinition
persistent identity: BBa K538303
display id: BBa_K538303
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBAD + RBS + CspA
creator: Bas Stringer
created: 2011-09-07T11:00:00Z
modified: 2015-05-08T01:12:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _704_
m user id: 0
owner id: 9985
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K538003
component: BBa_K206000
component: BBa_B0034
sequence annotation: BBa_K206000
sequence annotation: BBa_K538003
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K538303_sequence/1

BBa_K538003
type: Component
persistent identity: component2134097
display id: component2134097
version: 1
top level: pBAD:CspA
access: public
definition: https://synbiohub.org/public/igem/BBa_K538003/1

BBa_K206000
type: Component
persistent identity: component2134090
display id: component2134090
version: 1
top level: pBAD:CspA
access: public
definition: pBAD

BBa_B0034
type: Component
persistent identity: component2134092
display id: component2134092
version: 1
top level: pBAD:CspA
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K206000
type: SequenceAnnotation
persistent identity: annotation2134090
display id: annotation2134090
version: 1
top level: pBAD:CspA
location: https://synbiohub.org/public/igem/BBa_K538303/annotation2134090/range2134090/1
component: BBa_K206000

https://synbiohub.org/public/igem/BBa_K538303/annotation2134090/range2134090/1
type: Range
persistent identity: range2134090
display id: range2134090
version: 1
direction: direction/reverse
top level: pBAD:CspA
start: 1
end: 130
orientation: inline

BBa_K538003
type: SequenceAnnotation
persistent identity: annotation2134097
display id: annotation2134097
version: 1
top level: pBAD:CspA
location: https://synbiohub.org/public/igem/BBa_K538303/annotation2134097/range2134097/1
component: BBa_K538003

https://synbiohub.org/public/igem/BBa_K538303/annotation2134097/range2134097/1
type: Range
persistent identity: range2134097
display id: range2134097
version: 1
direction: direction/reverse
top level: pBAD:CspA
start: 157
end: 351
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2134092
display id: annotation2134092
version: 1
top level: pBAD:CspA
location: https://synbiohub.org/public/igem/BBa_K538303/annotation2134092/range2134092/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K538303/annotation2134092/range2134092/1
type: Range
persistent identity: range2134092
display id: range2134092
version: 1
direction: direction/reverse
top level: pBAD:CspA
start: 139
end: 150
orientation: inline

BBa_K112402
type: ComponentDefinition
persistent identity: BBa K112402
display id: BBa_K112402
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: promoter for FabA gene - Membrane Damage and Ultrasound Senstitive
creator: Christina Brown
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:17Z
discontinued: false
dominant: false
group u list: _224_
m user id: 0
owner id: 3241
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: According to Vollmer, et. al in their 1998 Applied and Enviormental Microbiology paper, "Bacterial Stress Responses to 1-Megahertz Pulsed Ultrasound in the Presence of Microbubble", the promoter for the fabA gene, a known membrane damage response gene, is upregulated seven fold when cells are exposed to high frequency ultrasound and microbubble cavitation.
mutable notes: none
mutable provenance: cloned from E. coli MG1655 genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K112402_sequence/1

P1
type: ComponentDefinition
persistent identity: BBa K100001
display id: BBa_K100001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Edited Xylose Regulated Bi-Directional Operator 1
creator: Garrett Tobin
created: 2008-08-17T11:00:00Z
modified: 2015-05-08T01:08:41Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _213_
m user id: 0
owner id: 1752
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part contains the operator region controlling transcription for the xyl operon. This regulatory region is dependent upon the binding of CRP-cAMP and XylR bound to xylose for activation. Upon binding, transcription is activated in both directions (xylFGH and xylAB facing). This part has a scrambled CRP binding site and and altered RNA ploymerase binding site that was designed to more resemble the consensus sequence slightly.
mutable notes: Both directions were kept in the sequence of the part even though it is intended to work in the right facing direction because of possible looping that may occur as a transcriptional control. The RNA polymerase binding site was altered to make binding stronger, attempting to allow activation when XylR-xylose alone is bound (the absence of CRP-cAMP).
mutable provenance: Taken from E. Coli genome bases 3728830-3729092, base pairs changed at RNA polymerase bining sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: RNA poly. Binding Site
sequence annotation: Scrambled CRP Binding Site
sequence: https://synbiohub.org/public/igem/BBa_K100001_sequence/1

RNA poly. Binding Site
type: SequenceAnnotation
persistent identity: annotation1972257
display id: annotation1972257
version: 1
top level: P1
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K100001/annotation1972257/range1972257/1

https://synbiohub.org/public/igem/BBa_K100001/annotation1972257/range1972257/1
type: Range
persistent identity: range1972257
display id: range1972257
version: 1
direction: direction/forward
top level: P1
start: 243
end: 248
orientation: inline

RNA poly. Binding Site
type: SequenceAnnotation
persistent identity: annotation1972254
display id: annotation1972254
version: 1
top level: P1
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K100001/annotation1972254/range1972254/1

https://synbiohub.org/public/igem/BBa_K100001/annotation1972254/range1972254/1
type: Range
persistent identity: range1972254
display id: range1972254
version: 1
direction: direction/reverse
top level: P1
start: 21
end: 26
orientation: inline

Scrambled CRP Binding Site
type: SequenceAnnotation
persistent identity: annotation1972256
display id: annotation1972256
version: 1
top level: P1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K100001/annotation1972256/range1972256/1

https://synbiohub.org/public/igem/BBa_K100001/annotation1972256/range1972256/1
type: Range
persistent identity: range1972256
display id: range1972256
version: 1
direction: direction/forward
top level: P1
start: 88
end: 104
orientation: inline

RNA poly. Binding Site
type: SequenceAnnotation
persistent identity: annotation1972255
display id: annotation1972255
version: 1
top level: P1
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K100001/annotation1972255/range1972255/1

https://synbiohub.org/public/igem/BBa_K100001/annotation1972255/range1972255/1
type: Range
persistent identity: range1972255
display id: range1972255
version: 1
direction: direction/reverse
top level: P1
start: 45
end: 50
orientation: inline

RNA poly. Binding Site
type: SequenceAnnotation
persistent identity: annotation1972258
display id: annotation1972258
version: 1
top level: P1
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K100001/annotation1972258/range1972258/1

https://synbiohub.org/public/igem/BBa_K100001/annotation1972258/range1972258/1
type: Range
persistent identity: range1972258
display id: range1972258
version: 1
direction: direction/forward
top level: P1
start: 265
end: 270
orientation: inline

BBa_J13210
type: ComponentDefinition
persistent identity: BBa J13210
display id: BBa_J13210
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pOmpR dependent POPS producer
creator: Jeff Tabor
created: 2005-07-18T11:00:00Z
modified: 2015-08-31T04:08:30Z
discontinued: false
dominant: false
experience: experience/None
group u list: _37_
m user id: 0
owner id: 88
owning group id: 37
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_B0010
component: BBa_B0012
component: BBa_R0082
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_R0082
sequence: https://synbiohub.org/public/igem/BBa_J13210_sequence/1

BBa_B0010
type: Component
persistent identity: component1587087
display id: component1587087
version: 1
top level: BBa_J13210
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1587097
display id: component1587097
version: 1
top level: BBa_J13210
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0082
type: Component
persistent identity: component1587117
display id: component1587117
version: 1
top level: BBa_J13210
access: public
definition: OmpR

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1587097
display id: annotation1587097
version: 1
top level: BBa_J13210
location: https://synbiohub.org/public/igem/BBa_J13210/annotation1587097/range1587097/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J13210/annotation1587097/range1587097/1
type: Range
persistent identity: range1587097
display id: range1587097
version: 1
direction: direction/forward
top level: BBa_J13210
start: 89
end: 129
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1587087
display id: annotation1587087
version: 1
top level: BBa_J13210
location: https://synbiohub.org/public/igem/BBa_J13210/annotation1587087/range1587087/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J13210/annotation1587087/range1587087/1
type: Range
persistent identity: range1587087
display id: range1587087
version: 1
direction: direction/forward
top level: BBa_J13210
start: 1
end: 80
orientation: inline

BBa_R0082
type: SequenceAnnotation
persistent identity: annotation1587117
display id: annotation1587117
version: 1
top level: BBa_J13210
location: https://synbiohub.org/public/igem/BBa_J13210/annotation1587117/range1587117/1
component: BBa_R0082

https://synbiohub.org/public/igem/BBa_J13210/annotation1587117/range1587117/1
type: Range
persistent identity: range1587117
display id: range1587117
version: 1
direction: direction/forward
top level: BBa_J13210
start: 138
end: 245
orientation: inline

BBa_J61055
type: ComponentDefinition
persistent identity: BBa J61055
display id: BBa_J61055
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [HIP-1fnr] Promoter
creator: John Anderson
created: 2007-02-20T12:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: non
mutable notes: non
mutable provenance: non
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J61055_sequence/1

BBa_K586000
type: ComponentDefinition
persistent identity: BBa K586000
display id: BBa_K586000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cup-1 Heavy metal sensor
creator: Chris Thompson
created: 2011-06-21T11:00:00Z
modified: 2015-05-08T01:12:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _757_
m user id: 0
owner id: 9336
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Cup-1 is a regulatory promoter that senses heavy metals such as copper, arsenic, cadmium, antimony, mercury, chromium, and selenium.
mutable notes: None
mutable provenance: Comes from genomic DNA of saccharomyces cerevisiae.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Cup-1
sequence: https://synbiohub.org/public/igem/BBa_K586000_sequence/1

Cup-1
type: SequenceAnnotation
persistent identity: annotation2122521
display id: annotation2122521
version: 1
top level: BBa_K586000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K586000/annotation2122521/range2122521/1

https://synbiohub.org/public/igem/BBa_K586000/annotation2122521/range2122521/1
type: Range
persistent identity: range2122521
display id: range2122521
version: 1
direction: direction/forward
top level: BBa_K586000
start: 1
end: 186
orientation: inline

BBa_I760005
type: ComponentDefinition
persistent identity: BBa I760005
display id: BBa_I760005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cu-sensitive promoter
creator: Gennady Zakharov
created: 2007-08-19T11:00:00Z
modified: 2015-08-31T04:08:08Z
discontinued: false
dominant: false
experience: experience/None
group u list: _148_
m user id: 0
owner id: 2080
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Promoter from the copper-sensetive CusR/CusS two component signal system. (E.Coli)
mutable notes: Sequence from the NCBI database, with added BioBricks prefix and suffix.
mutable provenance: http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I760005_sequence/1

BBa_K112320
type: ComponentDefinition
persistent identity: BBa K112320
display id: BBa_K112320
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {< ftsAZ promoter >} in BBb format
creator: Aron Lau
created: 2008-10-21T11:00:00Z
modified: 2015-05-08T01:09:16Z
discontinued: false
dominant: false
group u list: _224_
m user id: 0
owner id: 2999
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: ftsAZ promoter is a promoter with levels of expression are inversely proportional to the growth rate. This part is actually in BBb format. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: PCR al0021/al0022 on MG1655 (804bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112320 ----------------------------------------------- al0021 Forward Biobricking of ftsAZ promoter CCATAGAATTCatgAGATCTaacgacagtgttggtgagcg al0022 Reverse Biobricking of ftsAZ promoter CGttaGGATCCgtaccaatctccagtcctactaccag
mutable provenance: MG1655
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K112320_sequence/1

BBa_I739104
type: ComponentDefinition
persistent identity: BBa I739104
display id: BBa_I739104
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Double Promoter (LuxR/HSL, positive / P22 cII, negative)
creator: Stefan Luzi
created: 2007-10-14T11:00:00Z
modified: 2015-08-31T04:08:01Z
discontinued: false
dominant: false
group u list: _124_
m user id: 0
owner id: 2183
owning group id: 62
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: asdf
mutable notes: asdf
mutable provenance: Synthetic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Scar
sequence annotation: OR1
sequence annotation: R0053 (2x OR1)
sequence annotation: R0062
sequence: https://synbiohub.org/public/igem/BBa_I739104_sequence/1

Scar
type: SequenceAnnotation
persistent identity: annotation1947892
display id: annotation1947892
version: 1
top level: BBa_I739104
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I739104/annotation1947892/range1947892/1

https://synbiohub.org/public/igem/BBa_I739104/annotation1947892/range1947892/1
type: Range
persistent identity: range1947892
display id: range1947892
version: 1
direction: direction/forward
top level: BBa_I739104
start: 56
end: 63
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation1947893
display id: annotation1947893
version: 1
top level: BBa_I739104
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I739104/annotation1947893/range1947893/1

https://synbiohub.org/public/igem/BBa_I739104/annotation1947893/range1947893/1
type: Range
persistent identity: range1947893
display id: range1947893
version: 1
direction: direction/forward
top level: BBa_I739104
start: 64
end: 82
orientation: inline

R0053 (2x OR1)
type: SequenceAnnotation
persistent identity: annotation1947895
display id: annotation1947895
version: 1
top level: BBa_I739104
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I739104/annotation1947895/range1947895/1

https://synbiohub.org/public/igem/BBa_I739104/annotation1947895/range1947895/1
type: Range
persistent identity: range1947895
display id: range1947895
version: 1
direction: direction/forward
top level: BBa_I739104
start: 64
end: 101
orientation: inline

R0062
type: SequenceAnnotation
persistent identity: annotation1947891
display id: annotation1947891
version: 1
top level: BBa_I739104
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I739104/annotation1947891/range1947891/1

https://synbiohub.org/public/igem/BBa_I739104/annotation1947891/range1947891/1
type: Range
persistent identity: range1947891
display id: range1947891
version: 1
direction: direction/forward
top level: BBa_I739104
start: 1
end: 55
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation1947894
display id: annotation1947894
version: 1
top level: BBa_I739104
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I739104/annotation1947894/range1947894/1

https://synbiohub.org/public/igem/BBa_I739104/annotation1947894/range1947894/1
type: Range
persistent identity: range1947894
display id: range1947894
version: 1
direction: direction/forward
top level: BBa_I739104
start: 83
end: 101
orientation: inline

BBa_K1486047
type: ComponentDefinition
persistent identity: BBa K1486047
display id: BBa_K1486047
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CpxR promoter RV
creator: EPFL iGem team 2014
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: to edit
mutable notes: to edit
mutable provenance: to edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1486047_sequence/1

BBa_K1520010
type: ComponentDefinition
persistent identity: BBa K1520010
display id: BBa_K1520010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Prlux-rbs-rfp-Ter
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Prlux-rbs-rfp-Ter. It produces rfp when there is enough luxR and AHL.
mutable notes: None
mutable provenance: Prlux, rfp, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K1520500
component: BBa_K1520029
component: BBa_R0062
sequence annotation: BBa_K1520500
sequence annotation: BBa_K1520029
sequence annotation: BBa_R0062
sequence: https://synbiohub.org/public/igem/BBa_K1520010_sequence/1

BBa_K1520500
type: Component
persistent identity: component2384954
display id: component2384954
version: 1
top level: BBa_K1520010
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520500/1

BBa_K1520029
type: Component
persistent identity: component2384955
display id: component2384955
version: 1
top level: BBa_K1520010
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520029/1

BBa_R0062
type: Component
persistent identity: component2384949
display id: component2384949
version: 1
top level: BBa_K1520010
access: public
definition: lux pR

BBa_K1520500
type: SequenceAnnotation
persistent identity: annotation2384954
display id: annotation2384954
version: 1
top level: BBa_K1520010
location: https://synbiohub.org/public/igem/BBa_K1520010/annotation2384954/range2384954/1
component: BBa_K1520500

https://synbiohub.org/public/igem/BBa_K1520010/annotation2384954/range2384954/1
type: Range
persistent identity: range2384954
display id: range2384954
version: 1
direction: direction/reverse
top level: BBa_K1520010
start: 64
end: 795
orientation: inline

BBa_K1520029
type: SequenceAnnotation
persistent identity: annotation2384955
display id: annotation2384955
version: 1
top level: BBa_K1520010
location: https://synbiohub.org/public/igem/BBa_K1520010/annotation2384955/range2384955/1
component: BBa_K1520029

https://synbiohub.org/public/igem/BBa_K1520010/annotation2384955/range2384955/1
type: Range
persistent identity: range2384955
display id: range2384955
version: 1
direction: direction/reverse
top level: BBa_K1520010
start: 804
end: 932
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2384949
display id: annotation2384949
version: 1
top level: BBa_K1520010
location: https://synbiohub.org/public/igem/BBa_K1520010/annotation2384949/range2384949/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K1520010/annotation2384949/range2384949/1
type: Range
persistent identity: range2384949
display id: range2384949
version: 1
direction: direction/forward
top level: BBa_K1520010
start: 1
end: 55
orientation: inline

BBa_K180013
type: ComponentDefinition
persistent identity: BBa K180013
display id: BBa_K180013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Rock-paper-scissors - Rock secondary plasmid [rhl pR, LacI generator]
creator: Richard Wilson, Stephanie Chambers
created: 2009-10-18T11:00:00Z
modified: 2015-05-08T01:11:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _278_
m user id: 0
owner id: 4082
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: iGEM2009 Team:Southampton Game of Life secondary plasmid.
mutable notes: Regulated expression of LacI (C0012) by Rhl pR (R0071).
mutable provenance: See relevant basic biobrick parts (R0071, B0034, C0012, B0015)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0015
component: BBa_R0071
component: BBa_B0034
component: BBa_C0012
sequence annotation: BBa_C0012
sequence annotation: BBa_B0015
sequence annotation: BBa_R0071
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K180013_sequence/1

BBa_B0015
type: Component
persistent identity: component2256432
display id: component2256432
version: 1
top level: BBa_K180013
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_R0071
type: Component
persistent identity: component2256419
display id: component2256419
version: 1
top level: BBa_K180013
access: public
definition: RhlR+C4

BBa_B0034
type: Component
persistent identity: component2256421
display id: component2256421
version: 1
top level: BBa_K180013
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0012
type: Component
persistent identity: component2256423
display id: component2256423
version: 1
top level: BBa_K180013
access: public
definition: https://synbiohub.org/public/igem/BBa_C0012/1

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation2256423
display id: annotation2256423
version: 1
top level: BBa_K180013
location: https://synbiohub.org/public/igem/BBa_K180013/annotation2256423/range2256423/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_K180013/annotation2256423/range2256423/1
type: Range
persistent identity: range2256423
display id: range2256423
version: 1
direction: direction/forward
top level: BBa_K180013
start: 80
end: 1207
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2256432
display id: annotation2256432
version: 1
top level: BBa_K180013
location: https://synbiohub.org/public/igem/BBa_K180013/annotation2256432/range2256432/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K180013/annotation2256432/range2256432/1
type: Range
persistent identity: range2256432
display id: range2256432
version: 1
direction: direction/reverse
top level: BBa_K180013
start: 1241
end: 1369
orientation: inline

BBa_R0071
type: SequenceAnnotation
persistent identity: annotation2256419
display id: annotation2256419
version: 1
top level: BBa_K180013
location: https://synbiohub.org/public/igem/BBa_K180013/annotation2256419/range2256419/1
component: BBa_R0071

https://synbiohub.org/public/igem/BBa_K180013/annotation2256419/range2256419/1
type: Range
persistent identity: range2256419
display id: range2256419
version: 1
direction: direction/reverse
top level: BBa_K180013
start: 1
end: 53
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2256421
display id: annotation2256421
version: 1
top level: BBa_K180013
location: https://synbiohub.org/public/igem/BBa_K180013/annotation2256421/range2256421/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K180013/annotation2256421/range2256421/1
type: Range
persistent identity: range2256421
display id: range2256421
version: 1
direction: direction/reverse
top level: BBa_K180013
start: 62
end: 73
orientation: inline

pUV
type: ComponentDefinition
persistent identity: BBa I765001
display id: BBa_I765001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: UV promoter
creator: iGEM Colombian Team 2007
created: 2007-10-25T11:00:00Z
modified: 2015-08-31T04:08:09Z
discontinued: false
dominant: false
experience: experience/None
group u list: _118_
m user id: 0
owner id: 2266
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This device is regulated by UV irradiation
mutable notes: None
mutable provenance: Genomic sequence
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: promoter
sequence: https://synbiohub.org/public/igem/BBa_I765001_sequence/1

promoter
type: SequenceAnnotation
persistent identity: annotation1955941
display id: annotation1955941
version: 1
top level: pUV
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I765001/annotation1955941/range1955941/1

https://synbiohub.org/public/igem/BBa_I765001/annotation1955941/range1955941/1
type: Range
persistent identity: range1955941
display id: range1955941
version: 1
direction: direction/forward
top level: pUV
start: 1
end: 76
orientation: inline

BBa_K165041
type: ComponentDefinition
persistent identity: BBa K165041
display id: BBa_K165041
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Zif268-HIV binding sites + TEF constitutive yeast promoter
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K165011
component: BBa_K165037
sequence annotation: BBa_K165011
sequence annotation: BBa_K165037
sequence: https://synbiohub.org/public/igem/BBa_K165041_sequence/1

BBa_K165011
type: Component
persistent identity: component1996557
display id: component1996557
version: 1
top level: BBa_K165041
access: public
definition: https://synbiohub.org/public/igem/BBa_K165011/1

BBa_K165037
type: Component
persistent identity: component1996558
display id: component1996558
version: 1
top level: BBa_K165041
access: public
definition: https://synbiohub.org/public/igem/BBa_K165037/1

BBa_K165011
type: SequenceAnnotation
persistent identity: annotation1996557
display id: annotation1996557
version: 1
top level: BBa_K165041
location: https://synbiohub.org/public/igem/BBa_K165041/annotation1996557/range1996557/1
component: BBa_K165011

https://synbiohub.org/public/igem/BBa_K165041/annotation1996557/range1996557/1
type: Range
persistent identity: range1996557
display id: range1996557
version: 1
direction: direction/reverse
top level: BBa_K165041
start: 1
end: 46
orientation: inline

BBa_K165037
type: SequenceAnnotation
persistent identity: annotation1996558
display id: annotation1996558
version: 1
top level: BBa_K165041
location: https://synbiohub.org/public/igem/BBa_K165041/annotation1996558/range1996558/1
component: BBa_K165037

https://synbiohub.org/public/igem/BBa_K165041/annotation1996558/range1996558/1
type: Range
persistent identity: range1996558
display id: range1996558
version: 1
direction: direction/reverse
top level: BBa_K165041
start: 55
end: 457
orientation: inline

BBa_I731004
type: ComponentDefinition
persistent identity: BBa I731004
display id: BBa_I731004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FecA promoter
creator: Shaunak Vankudre
created: 2007-06-25T11:00:00Z
modified: 2015-08-31T04:07:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _127_
m user id: 0
owner id: 1889
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: derived from Enz et al, 2003, J Bacteriol. 2003 April; 185(7): 2387???2391. doi: 10.1128/JB.185.7.2387-2391.2003.
mutable provenance: genomic sequence from E. Coli K12
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I731004_sequence/1

BBa_K110014
type: ComponentDefinition
persistent identity: BBa K110014
display id: BBa_K110014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: A-Cell Promoter MFA2 (backwards)
creator: James DiCarlo
created: 2008-08-26T11:00:00Z
modified: 2015-05-08T01:09:09Z
discontinued: false
dominant: false
experience: experience/None
group u list: _201_
m user id: 0
owner id: 2669
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the same part as BBa_K110007 except it has Prefix and Suffix regions switched
mutable notes: This was taken from SGD
mutable provenance: SGD
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K110014_sequence/1

BBa_J24813
type: ComponentDefinition
persistent identity: BBa J24813
display id: BBa_J24813
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: URA3 Promoter from S. cerevisiae
creator: John Szymanski
created: 2008-05-01T11:00:00Z
modified: 2015-08-31T04:08:43Z
discontinued: false
dominant: false
group u list: _58_
m user id: 0
owner id: 2512
owning group id: 58
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the whole regulatory region from the URA3 gene coding for OMP decarboxylase in S. cerevisiae budding yeast.
mutable notes: None
mutable provenance: NCBI Sequence database: http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=Nucleotide&dopt=GenBank&val=172061
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J24813_sequence/1

VirA/G
type: ComponentDefinition
persistent identity: BBa K389017
display id: BBa_K389017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: VirA/G signaling system without read-out
creator: Jonas Aretz
created: 2010-09-27T11:00:00Z
modified: 2015-05-08T01:12:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This BioBrick is a virA/G signaling system without read-out. It contains an unmutated ''virA'' gene and a ''virG'' gene that works without ''rpoA'' from ''A. tumefaciens'' and a ''vir'' promoter.
mutable notes: This BioBrick can be used to control any gene with a ''vir'' promoter
mutable provenance: ''A. tumefaciens'' C58 TI-plasmid, partsregistry, Mr. Gene
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_B0017
component: BBa_K389010
component: BBa_J23110
component: BBa_K389003
component: BBa_K389002
sequence annotation: BBa_J23110
sequence annotation: BBa_K389002
sequence annotation: BBa_K389003
sequence annotation: BBa_B0017
sequence annotation: BBa_K389010
sequence: https://synbiohub.org/public/igem/BBa_K389017_sequence/1

BBa_B0017
type: Component
persistent identity: component2226706
display id: component2226706
version: 1
top level: VirA/G
access: public
definition: https://synbiohub.org/public/igem/BBa_B0017/1

BBa_K389010
type: Component
persistent identity: component2226696
display id: component2226696
version: 1
top level: VirA/G
access: public
definition: https://synbiohub.org/public/igem/BBa_K389010/1

BBa_J23110
type: Component
persistent identity: component2226697
display id: component2226697
version: 1
top level: VirA/G
access: public
definition: https://synbiohub.org/public/igem/BBa_J23110/1

BBa_K389003
type: Component
persistent identity: component2226711
display id: component2226711
version: 1
top level: VirA/G
access: public
definition: Pvir

BBa_K389002
type: Component
persistent identity: component2226701
display id: component2226701
version: 1
top level: VirA/G
access: public
definition: https://synbiohub.org/public/igem/BBa_K389002/1

BBa_J23110
type: SequenceAnnotation
persistent identity: annotation2226697
display id: annotation2226697
version: 1
top level: VirA/G
location: https://synbiohub.org/public/igem/BBa_K389017/annotation2226697/range2226697/1
component: BBa_J23110

https://synbiohub.org/public/igem/BBa_K389017/annotation2226697/range2226697/1
type: Range
persistent identity: range2226697
display id: range2226697
version: 1
direction: direction/reverse
top level: VirA/G
start: 2575
end: 2609
orientation: inline

BBa_K389002
type: SequenceAnnotation
persistent identity: annotation2226701
display id: annotation2226701
version: 1
top level: VirA/G
location: https://synbiohub.org/public/igem/BBa_K389017/annotation2226701/range2226701/1
component: BBa_K389002

https://synbiohub.org/public/igem/BBa_K389017/annotation2226701/range2226701/1
type: Range
persistent identity: range2226701
display id: range2226701
version: 1
direction: direction/reverse
top level: VirA/G
start: 2618
end: 3357
orientation: inline

BBa_K389003
type: SequenceAnnotation
persistent identity: annotation2226711
display id: annotation2226711
version: 1
top level: VirA/G
location: https://synbiohub.org/public/igem/BBa_K389017/annotation2226711/range2226711/1
component: BBa_K389003

https://synbiohub.org/public/igem/BBa_K389017/annotation2226711/range2226711/1
type: Range
persistent identity: range2226711
display id: range2226711
version: 1
direction: direction/reverse
top level: VirA/G
start: 3542
end: 3699
orientation: inline

BBa_B0017
type: SequenceAnnotation
persistent identity: annotation2226706
display id: annotation2226706
version: 1
top level: VirA/G
location: https://synbiohub.org/public/igem/BBa_K389017/annotation2226706/range2226706/1
component: BBa_B0017

https://synbiohub.org/public/igem/BBa_K389017/annotation2226706/range2226706/1
type: Range
persistent identity: range2226706
display id: range2226706
version: 1
direction: direction/reverse
top level: VirA/G
start: 3366
end: 3533
orientation: inline

BBa_K389010
type: SequenceAnnotation
persistent identity: annotation2226696
display id: annotation2226696
version: 1
top level: VirA/G
location: https://synbiohub.org/public/igem/BBa_K389017/annotation2226696/range2226696/1
component: BBa_K389010

https://synbiohub.org/public/igem/BBa_K389017/annotation2226696/range2226696/1
type: Range
persistent identity: range2226696
display id: range2226696
version: 1
direction: direction/reverse
top level: VirA/G
start: 1
end: 2566
orientation: inline

Prm +
type: ComponentDefinition
persistent identity: BBa I12006
display id: BBa_I12006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Modified lamdba Prm promoter (repressed by 434 cI)
creator: mcnamara
created: 2004-07-13T11:00:00Z
modified: 2015-08-31T04:07:31Z
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 147
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Lamdba Prm promoter modified to be activated by lamda repressor (cI) and repressed by 434 repressor (cI)
mutable notes: The O-R1 region of 434 contained 14 base pairs as opposed to the 17 base pairs of the O-R3 site of lambda. Also, it was noticed that the O-R3 site of the lambda included part of the -10 site. Hence, to preserve the spacing and the -10 site, the three nucleotides that were in both the -10 site and the lambda O-R3 site were retained. The 14 nucleotides that were in the O-R3 site and not in the -10 site were replaced with the O-R1 site of the 434.
mutable provenance: Bushman(1993), Shih & Gussin (1983)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: OR2 lambda
sequence annotation: OR1 lambda
sequence annotation: OR1 434
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_I12006_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation837228
display id: annotation837228
version: 1
top level: Prm +
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I12006/annotation837228/range837228/1

https://synbiohub.org/public/igem/BBa_I12006/annotation837228/range837228/1
type: Range
persistent identity: range837228
display id: range837228
version: 1
direction: direction/forward
top level: Prm +
start: 71
end: 76
orientation: inline

OR2 lambda
type: SequenceAnnotation
persistent identity: annotation786518
display id: annotation786518
version: 1
top level: Prm +
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12006/annotation786518/range786518/1

https://synbiohub.org/public/igem/BBa_I12006/annotation786518/range786518/1
type: Range
persistent identity: range786518
display id: range786518
version: 1
direction: direction/forward
top level: Prm +
start: 33
end: 49
orientation: inline

OR1 lambda
type: SequenceAnnotation
persistent identity: annotation786500
display id: annotation786500
version: 1
top level: Prm +
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12006/annotation786500/range786500/1

https://synbiohub.org/public/igem/BBa_I12006/annotation786500/range786500/1
type: Range
persistent identity: range786500
display id: range786500
version: 1
direction: direction/forward
top level: Prm +
start: 9
end: 25
orientation: inline

OR1 434
type: SequenceAnnotation
persistent identity: annotation786365
display id: annotation786365
version: 1
top level: Prm +
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12006/annotation786365/range786365/1

https://synbiohub.org/public/igem/BBa_I12006/annotation786365/range786365/1
type: Range
persistent identity: range786365
display id: range786365
version: 1
direction: direction/forward
top level: Prm +
start: 56
end: 69
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation837284
display id: annotation837284
version: 1
top level: Prm +
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I12006/annotation837284/range837284/1

https://synbiohub.org/public/igem/BBa_I12006/annotation837284/range837284/1
type: Range
persistent identity: range837284
display id: range837284
version: 1
direction: direction/forward
top level: Prm +
start: 48
end: 53
orientation: inline

BBa_K259005
type: ComponentDefinition
persistent identity: BBa K259005
display id: BBa_K259005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AraC Rheostat Promoter
creator: Petros Mina
created: 2009-09-02T11:00:00Z
modified: 2015-05-08T01:11:42Z
discontinued: false
dominant: false
group u list: _357_
m user id: 0
owner id: 5318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The AraC/pBAD promoter can be used with prokaryotic chassis to regulate expresion of proteins. When the AraC/pBAD promoter is placed upstream of your canditate translational unit (protein to be expressed) it allows control of the expression of the said protein. This is done via controlling the concentration of arabinose sugar in the environment/solution of bacterial growth. This composite part does not have an LVA degradation tag at its end, hence can be used with all prokaryotic assemblies.
mutable notes: The part was designed and assembled from the iGEM2009 kit with standard RFC10 assembly.
mutable provenance: The AraC promoter is found naturally in E.Coli at 1.52 min/centisome in the clockwise orientation. More information can be found at [http://ecogene.org/geneinfo.php?eg_id=EG10054 EcoGene].
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0081
component: BBa_R0080
sequence annotation: BBa_R0080
sequence annotation: BBa_R0081
sequence: https://synbiohub.org/public/igem/BBa_K259005_sequence/1

BBa_R0081
type: Component
persistent identity: component2018287
display id: component2018287
version: 1
top level: BBa_K259005
access: public
definition: https://synbiohub.org/public/igem/BBa_R0081/1

BBa_R0080
type: Component
persistent identity: component2018294
display id: component2018294
version: 1
top level: BBa_K259005
access: public
definition: AraC

BBa_R0080
type: SequenceAnnotation
persistent identity: annotation2018294
display id: annotation2018294
version: 1
top level: BBa_K259005
location: https://synbiohub.org/public/igem/BBa_K259005/annotation2018294/range2018294/1
component: BBa_R0080

https://synbiohub.org/public/igem/BBa_K259005/annotation2018294/range2018294/1
type: Range
persistent identity: range2018294
display id: range2018294
version: 1
direction: direction/reverse
top level: BBa_K259005
start: 192
end: 340
orientation: inline

BBa_R0081
type: SequenceAnnotation
persistent identity: annotation2018287
display id: annotation2018287
version: 1
top level: BBa_K259005
location: https://synbiohub.org/public/igem/BBa_K259005/annotation2018287/range2018287/1
component: BBa_R0081

https://synbiohub.org/public/igem/BBa_K259005/annotation2018287/range2018287/1
type: Range
persistent identity: range2018287
display id: range2018287
version: 1
direction: direction/reverse
top level: BBa_K259005
start: 1
end: 183
orientation: inline

BBa_K338081
type: ComponentDefinition
persistent identity: BBa K338081
display id: BBa_K338081
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Heat-Shock Activated Generator
creator: Lucas Hartsough
created: 2010-10-26T11:00:00Z
modified: 2015-05-08T01:12:07Z
discontinued: false
dominant: false
group u list: _454_
m user id: 0
owner id: 6013
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Intermediate designed to accelerate cloning of the HSP promoter ( BBa_K338001 ) by adding an RBS downstream.
mutable notes: Designed to be ligated upstream of the desired heat-shock product. Keep in mind that the promoter is somewhat leaky and that cultures should be grown at 30°C.
mutable provenance: The Registry of Standard Biological Parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_K338001
sequence annotation: BBa_B0034
sequence annotation: BBa_K338001
sequence: https://synbiohub.org/public/igem/BBa_K338081_sequence/1

BBa_B0034
type: Component
persistent identity: component2105680
display id: component2105680
version: 1
top level: BBa_K338081
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K338001
type: Component
persistent identity: component2105678
display id: component2105678
version: 1
top level: BBa_K338081
access: public
definition: HSP

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2105680
display id: annotation2105680
version: 1
top level: BBa_K338081
location: https://synbiohub.org/public/igem/BBa_K338081/annotation2105680/range2105680/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K338081/annotation2105680/range2105680/1
type: Range
persistent identity: range2105680
display id: range2105680
version: 1
direction: direction/reverse
top level: BBa_K338081
start: 107
end: 118
orientation: inline

BBa_K338001
type: SequenceAnnotation
persistent identity: annotation2105678
display id: annotation2105678
version: 1
top level: BBa_K338081
location: https://synbiohub.org/public/igem/BBa_K338081/annotation2105678/range2105678/1
component: BBa_K338001

https://synbiohub.org/public/igem/BBa_K338081/annotation2105678/range2105678/1
type: Range
persistent identity: range2105678
display id: range2105678
version: 1
direction: direction/reverse
top level: BBa_K338081
start: 1
end: 98
orientation: inline

BBa_K136010
type: ComponentDefinition
persistent identity: BBa K136010
display id: BBa_K136010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: fliA promoter
creator: Kok-Phen YAN
created: 2008-10-24T11:00:00Z
modified: 2015-05-08T01:10:02Z
discontinued: false
dominant: false
group u list: _211_
m user id: 0
owner id: 3262
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: FliA is a transcription factor involved in the formation of the flagella in E. coli. The promotor of fliA is induced by FlhDC. FliA seems to have a positive autoregulation on its promotor.
mutable notes: primers used
mutable provenance: This part comes from the genome of E. coli K12 strain MG1655.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: ATG
sequence annotation: natural fliA RBS
sequence annotation: FlhDC binding site 1
sequence annotation: A mutated to G
sequence annotation: transcription initiation site
sequence annotation: FlhDC binding site 2
sequence: https://synbiohub.org/public/igem/BBa_K136010_sequence/1

ATG
type: SequenceAnnotation
persistent identity: annotation1987044
display id: annotation1987044
version: 1
top level: BBa_K136010
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K136010/annotation1987044/range1987044/1

https://synbiohub.org/public/igem/BBa_K136010/annotation1987044/range1987044/1
type: Range
persistent identity: range1987044
display id: range1987044
version: 1
direction: direction/forward
top level: BBa_K136010
start: 342
end: 344
orientation: inline

natural fliA RBS
type: SequenceAnnotation
persistent identity: annotation1999348
display id: annotation1999348
version: 1
top level: BBa_K136010
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K136010/annotation1999348/range1999348/1

https://synbiohub.org/public/igem/BBa_K136010/annotation1999348/range1999348/1
type: Range
persistent identity: range1999348
display id: range1999348
version: 1
direction: direction/forward
top level: BBa_K136010
start: 330
end: 337
orientation: inline

FlhDC binding site 1
type: SequenceAnnotation
persistent identity: annotation1987040
display id: annotation1987040
version: 1
top level: BBa_K136010
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K136010/annotation1987040/range1987040/1

https://synbiohub.org/public/igem/BBa_K136010/annotation1987040/range1987040/1
type: Range
persistent identity: range1987040
display id: range1987040
version: 1
direction: direction/forward
top level: BBa_K136010
start: 198
end: 225
orientation: inline

A mutated to G
type: SequenceAnnotation
persistent identity: annotation1987042
display id: annotation1987042
version: 1
top level: BBa_K136010
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K136010/annotation1987042/range1987042/1

https://synbiohub.org/public/igem/BBa_K136010/annotation1987042/range1987042/1
type: Range
persistent identity: range1987042
display id: range1987042
version: 1
direction: direction/forward
top level: BBa_K136010
start: 263
end: 263
orientation: inline

A mutated to G
type: SequenceAnnotation
persistent identity: annotation1987043
display id: annotation1987043
version: 1
top level: BBa_K136010
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K136010/annotation1987043/range1987043/1

https://synbiohub.org/public/igem/BBa_K136010/annotation1987043/range1987043/1
type: Range
persistent identity: range1987043
display id: range1987043
version: 1
direction: direction/forward
top level: BBa_K136010
start: 316
end: 316
orientation: inline

transcription initiation site
type: SequenceAnnotation
persistent identity: annotation1987045
display id: annotation1987045
version: 1
top level: BBa_K136010
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K136010/annotation1987045/range1987045/1

https://synbiohub.org/public/igem/BBa_K136010/annotation1987045/range1987045/1
type: Range
persistent identity: range1987045
display id: range1987045
version: 1
direction: direction/forward
top level: BBa_K136010
start: 282
end: 282
orientation: inline

FlhDC binding site 2
type: SequenceAnnotation
persistent identity: annotation1987041
display id: annotation1987041
version: 1
top level: BBa_K136010
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K136010/annotation1987041/range1987041/1

https://synbiohub.org/public/igem/BBa_K136010/annotation1987041/range1987041/1
type: Range
persistent identity: range1987041
display id: range1987041
version: 1
direction: direction/forward
top level: BBa_K136010
start: 226
end: 261
orientation: inline

transcription initiation site
type: SequenceAnnotation
persistent identity: annotation1987046
display id: annotation1987046
version: 1
top level: BBa_K136010
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K136010/annotation1987046/range1987046/1

https://synbiohub.org/public/igem/BBa_K136010/annotation1987046/range1987046/1
type: Range
persistent identity: range1987046
display id: range1987046
version: 1
direction: direction/forward
top level: BBa_K136010
start: 293
end: 293
orientation: inline

glnAp2 pro
type: ComponentDefinition
persistent identity: BBa J64979
display id: BBa_J64979
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: glnAp2
creator: Kristopher Kuchenbecker
created: 2007-03-25T12:00:00Z
modified: 2015-05-08T01:08:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 1429
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This promoter sequence (-80 to +1) is responsive to the transcription enhancer glnG.
mutable notes: n/a
mutable provenance: ''E. coli''
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J64979_sequence/1

BBa_K116401
type: ComponentDefinition
persistent identity: BBa K116401
display id: BBa_K116401
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: external phosphate sensing promoter
creator: Chih-Hsien Yang
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:09:32Z
discontinued: false
dominant: false
group u list: _210_
m user id: 0
owner id: 592
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: promoter of phoB in E.coli, an external phosphate regulated promoter which can be activated when encountering phosphate limitation.
mutable notes: The promoter region should contain PhoB binding site
mutable provenance: E. coli K12 genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: PhoB binding site
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_K116401_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation1995352
display id: annotation1995352
version: 1
top level: BBa_K116401
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K116401/annotation1995352/range1995352/1

https://synbiohub.org/public/igem/BBa_K116401/annotation1995352/range1995352/1
type: Range
persistent identity: range1995352
display id: range1995352
version: 1
direction: direction/forward
top level: BBa_K116401
start: 421
end: 429
orientation: inline

PhoB binding site
type: SequenceAnnotation
persistent identity: annotation1995351
display id: annotation1995351
version: 1
top level: BBa_K116401
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K116401/annotation1995351/range1995351/1

https://synbiohub.org/public/igem/BBa_K116401/annotation1995351/range1995351/1
type: Range
persistent identity: range1995351
display id: range1995351
version: 1
direction: direction/forward
top level: BBa_K116401
start: 420
end: 439
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1995353
display id: annotation1995353
version: 1
top level: BBa_K116401
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K116401/annotation1995353/range1995353/1

https://synbiohub.org/public/igem/BBa_K116401/annotation1995353/range1995353/1
type: Range
persistent identity: range1995353
display id: range1995353
version: 1
direction: direction/forward
top level: BBa_K116401
start: 448
end: 455
orientation: inline

Lux
type: ComponentDefinition
persistent identity: BBa I1051
display id: BBa_I1051
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lux cassette right promoter
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:30Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux repressor, LuxR. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.In thisversion, a binding site for cI dimer has been added: binding to this site dominantly represses transcription.
mutable provenance: V. fishceri and phage lambda (courtesy of Ron Weiss)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_I1051
sequence annotation: LuxR/HSL
sequence annotation: cI (OR1)
sequence annotation: -35
sequence annotation: -10
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_I1051_sequence/1

BBa_I1051
type: SequenceAnnotation
persistent identity: annotation7059
display id: annotation7059
version: 1
top level: Lux
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I1051/annotation7059/range7059/1

https://synbiohub.org/public/igem/BBa_I1051/annotation7059/range7059/1
type: Range
persistent identity: range7059
display id: range7059
version: 1
direction: direction/forward
top level: Lux
start: 1
end: 68
orientation: inline

LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation1936
display id: annotation1936
version: 1
top level: Lux
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I1051/annotation1936/range1936/1

https://synbiohub.org/public/igem/BBa_I1051/annotation1936/range1936/1
type: Range
persistent identity: range1936
display id: range1936
version: 1
direction: direction/forward
top level: Lux
start: 1
end: 20
orientation: inline

cI (OR1)
type: SequenceAnnotation
persistent identity: annotation1939
display id: annotation1939
version: 1
top level: Lux
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I1051/annotation1939/range1939/1

https://synbiohub.org/public/igem/BBa_I1051/annotation1939/range1939/1
type: Range
persistent identity: range1939
display id: range1939
version: 1
direction: direction/forward
top level: Lux
start: 52
end: 68
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1937
display id: annotation1937
version: 1
top level: Lux
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I1051/annotation1937/range1937/1

https://synbiohub.org/public/igem/BBa_I1051/annotation1937/range1937/1
type: Range
persistent identity: range1937
display id: range1937
version: 1
direction: direction/forward
top level: Lux
start: 20
end: 25
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1938
display id: annotation1938
version: 1
top level: Lux
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I1051/annotation1938/range1938/1

https://synbiohub.org/public/igem/BBa_I1051/annotation1938/range1938/1
type: Range
persistent identity: range1938
display id: range1938
version: 1
direction: direction/forward
top level: Lux
start: 42
end: 47
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1940
display id: annotation1940
version: 1
top level: Lux
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I1051/annotation1940/range1940/1

https://synbiohub.org/public/igem/BBa_I1051/annotation1940/range1940/1
type: Range
persistent identity: range1940
display id: range1940
version: 1
direction: direction/forward
top level: Lux
start: 53
end: 53
orientation: inline

pRE
type: ComponentDefinition
persistent identity: BBa K116603
display id: BBa_K116603
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pRE promoter from λ phage
creator: Jesse Wu
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:09:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _210_
m user id: 0
owner id: 2749
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The pRE coding region from λ phage. It is able to be induced by CII (BBa_K116602).
mutable notes: none.
mutable provenance: λ phage
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K116603_sequence/1

BBa_K110015
type: ComponentDefinition
persistent identity: BBa K110015
display id: BBa_K110015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: A-Cell Promoter MFA1 (RtL)
creator: Rick Carrick, Ambhi Ganesan, Alex McMillan
created: 2008-08-26T11:00:00Z
modified: 2015-05-08T01:09:09Z
discontinued: false
dominant: false
experience: experience/None
group u list: _201_
m user id: 0
owner id: 2669
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the same sequence as MFA1 promotor(BBa_K110008), but with suffix and prefix switched
mutable notes: This is directlyh from the DNA sequence
mutable provenance: sgd
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: MCM1
sequence annotation: STE12
sequence annotation: MATalpha2
sequence annotation: MFA1 promoter (RtL)
sequence: https://synbiohub.org/public/igem/BBa_K110015_sequence/1

MCM1
type: SequenceAnnotation
persistent identity: annotation1981505
display id: annotation1981505
version: 1
top level: BBa_K110015
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110015/annotation1981505/range1981505/1

https://synbiohub.org/public/igem/BBa_K110015/annotation1981505/range1981505/1
type: Range
persistent identity: range1981505
display id: range1981505
version: 1
direction: direction/forward
top level: BBa_K110015
start: 277
end: 287
orientation: inline

STE12
type: SequenceAnnotation
persistent identity: annotation1981506
display id: annotation1981506
version: 1
top level: BBa_K110015
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110015/annotation1981506/range1981506/1

https://synbiohub.org/public/igem/BBa_K110015/annotation1981506/range1981506/1
type: Range
persistent identity: range1981506
display id: range1981506
version: 1
direction: direction/forward
top level: BBa_K110015
start: 302
end: 309
orientation: inline

MATalpha2
type: SequenceAnnotation
persistent identity: annotation1981504
display id: annotation1981504
version: 1
top level: BBa_K110015
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110015/annotation1981504/range1981504/1

https://synbiohub.org/public/igem/BBa_K110015/annotation1981504/range1981504/1
type: Range
persistent identity: range1981504
display id: range1981504
version: 1
direction: direction/forward
top level: BBa_K110015
start: 267
end: 276
orientation: inline

MATalpha2
type: SequenceAnnotation
persistent identity: annotation1981507
display id: annotation1981507
version: 1
top level: BBa_K110015
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110015/annotation1981507/range1981507/1

https://synbiohub.org/public/igem/BBa_K110015/annotation1981507/range1981507/1
type: Range
persistent identity: range1981507
display id: range1981507
version: 1
direction: direction/forward
top level: BBa_K110015
start: 289
end: 297
orientation: inline

MFA1 promoter (RtL)
type: SequenceAnnotation
persistent identity: annotation1981503
display id: annotation1981503
version: 1
top level: BBa_K110015
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K110015/annotation1981503/range1981503/1

https://synbiohub.org/public/igem/BBa_K110015/annotation1981503/range1981503/1
type: Range
persistent identity: range1981503
display id: range1981503
version: 1
direction: direction/forward
top level: BBa_K110015
start: 1
end: 401
orientation: inline

BBa_K180002
type: ComponentDefinition
persistent identity: BBa K180002
display id: BBa_K180002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tac pR testing plasmid (GFP)
creator: Richard Wilson, Stephanie Chambers
created: 2009-10-13T11:00:00Z
modified: 2015-05-08T01:11:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _278_
m user id: 0
owner id: 4082
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: PTAC promoter followed by an RBS, green fluorescent protein and stop codon, for testing the effectiveness of the PTAC hybrid promoter designed by iGEM2009 Team:Southampton.
mutable notes: Inclusion of the RBS and stop sequence to ensure correct functionality observed.
mutable provenance: K180000 and existing biobrick parts.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_E0040
component: BBa_B0012
component: BBa_K180000
component: BBa_B0034
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_K180000
sequence annotation: BBa_E0040
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K180002_sequence/1

BBa_E0040
type: Component
persistent identity: component2037771
display id: component2037771
version: 1
top level: BBa_K180002
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0012
type: Component
persistent identity: component2037774
display id: component2037774
version: 1
top level: BBa_K180002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_K180000
type: Component
persistent identity: component2037767
display id: component2037767
version: 1
top level: BBa_K180002
access: public
definition: BBa_K180000

BBa_B0034
type: Component
persistent identity: component2037769
display id: component2037769
version: 1
top level: BBa_K180002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component2037772
display id: component2037772
version: 1
top level: BBa_K180002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2037772
display id: annotation2037772
version: 1
top level: BBa_K180002
location: https://synbiohub.org/public/igem/BBa_K180002/annotation2037772/range2037772/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K180002/annotation2037772/range2037772/1
type: Range
persistent identity: range2037772
display id: range2037772
version: 1
direction: direction/forward
top level: BBa_K180002
start: 2202
end: 2281
orientation: inline

BBa_K180000
type: SequenceAnnotation
persistent identity: annotation2037767
display id: annotation2037767
version: 1
top level: BBa_K180002
location: https://synbiohub.org/public/igem/BBa_K180002/annotation2037767/range2037767/1
component: BBa_K180000

https://synbiohub.org/public/igem/BBa_K180002/annotation2037767/range2037767/1
type: Range
persistent identity: range2037767
display id: range2037767
version: 1
direction: direction/reverse
top level: BBa_K180002
start: 1
end: 1447
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2037771
display id: annotation2037771
version: 1
top level: BBa_K180002
location: https://synbiohub.org/public/igem/BBa_K180002/annotation2037771/range2037771/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K180002/annotation2037771/range2037771/1
type: Range
persistent identity: range2037771
display id: range2037771
version: 1
direction: direction/reverse
top level: BBa_K180002
start: 1474
end: 2193
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2037774
display id: annotation2037774
version: 1
top level: BBa_K180002
location: https://synbiohub.org/public/igem/BBa_K180002/annotation2037774/range2037774/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K180002/annotation2037774/range2037774/1
type: Range
persistent identity: range2037774
display id: range2037774
version: 1
direction: direction/forward
top level: BBa_K180002
start: 2290
end: 2330
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2037769
display id: annotation2037769
version: 1
top level: BBa_K180002
location: https://synbiohub.org/public/igem/BBa_K180002/annotation2037769/range2037769/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K180002/annotation2037769/range2037769/1
type: Range
persistent identity: range2037769
display id: range2037769
version: 1
direction: direction/reverse
top level: BBa_K180002
start: 1456
end: 1467
orientation: inline

BBa_I739105
type: ComponentDefinition
persistent identity: BBa I739105
display id: BBa_I739105
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Double Promoter (LuxR/HSL, positive / cI, negative)
creator: Stefan Luzi
created: 2007-10-14T11:00:00Z
modified: 2015-08-31T04:08:01Z
discontinued: false
dominant: false
experience: experience/None
group u list: _124_
m user id: 0
owner id: 2183
owning group id: 62
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: asd
mutable notes: asdf
mutable provenance: Synthetic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OR2
sequence annotation: R0062
sequence annotation: Scar
sequence annotation: R0051 (2x OR2)
sequence: https://synbiohub.org/public/igem/BBa_I739105_sequence/1

OR2
type: SequenceAnnotation
persistent identity: annotation1947917
display id: annotation1947917
version: 1
top level: BBa_I739105
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I739105/annotation1947917/range1947917/1

https://synbiohub.org/public/igem/BBa_I739105/annotation1947917/range1947917/1
type: Range
persistent identity: range1947917
display id: range1947917
version: 1
direction: direction/forward
top level: BBa_I739105
start: 82
end: 99
orientation: inline

R0062
type: SequenceAnnotation
persistent identity: annotation1947914
display id: annotation1947914
version: 1
top level: BBa_I739105
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I739105/annotation1947914/range1947914/1

https://synbiohub.org/public/igem/BBa_I739105/annotation1947914/range1947914/1
type: Range
persistent identity: range1947914
display id: range1947914
version: 1
direction: direction/forward
top level: BBa_I739105
start: 1
end: 55
orientation: inline

Scar
type: SequenceAnnotation
persistent identity: annotation1947915
display id: annotation1947915
version: 1
top level: BBa_I739105
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I739105/annotation1947915/range1947915/1

https://synbiohub.org/public/igem/BBa_I739105/annotation1947915/range1947915/1
type: Range
persistent identity: range1947915
display id: range1947915
version: 1
direction: direction/forward
top level: BBa_I739105
start: 56
end: 63
orientation: inline

R0051 (2x OR2)
type: SequenceAnnotation
persistent identity: annotation1947918
display id: annotation1947918
version: 1
top level: BBa_I739105
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I739105/annotation1947918/range1947918/1

https://synbiohub.org/public/igem/BBa_I739105/annotation1947918/range1947918/1
type: Range
persistent identity: range1947918
display id: range1947918
version: 1
direction: direction/forward
top level: BBa_I739105
start: 64
end: 99
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation1947916
display id: annotation1947916
version: 1
top level: BBa_I739105
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I739105/annotation1947916/range1947916/1

https://synbiohub.org/public/igem/BBa_I739105/annotation1947916/range1947916/1
type: Range
persistent identity: range1947916
display id: range1947916
version: 1
direction: direction/forward
top level: BBa_I739105
start: 64
end: 81
orientation: inline

BBa_K206001
type: ComponentDefinition
persistent identity: BBa K206001
display id: BBa_K206001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBAD weak
creator: Amelia Hardjasa
created: 2009-10-16T11:00:00Z
modified: 2015-05-08T01:11:23Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _307_
m user id: 0
owner id: 4172
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Weaker version of wild type pBAD ( I13453 ).
mutable notes: No special considerations
mutable provenance: Site-directed mutagenesis on I13453 with the following primers: Forward: TAATCTTATGGATAAAAAAGCTATGGCATAGC Reverse: GCGGATCCTACCTGACGCTTTTTATC
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: promoter
sequence annotation: AraI2
sequence annotation: AraI1
sequence: https://synbiohub.org/public/igem/BBa_K206001_sequence/1

promoter
type: SequenceAnnotation
persistent identity: annotation2049255
display id: annotation2049255
version: 1
top level: BBa_K206001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K206001/annotation2049255/range2049255/1

https://synbiohub.org/public/igem/BBa_K206001/annotation2049255/range2049255/1
type: Range
persistent identity: range2049255
display id: range2049255
version: 1
direction: direction/forward
top level: BBa_K206001
start: 1
end: 131
orientation: inline

AraI2
type: SequenceAnnotation
persistent identity: annotation2049257
display id: annotation2049257
version: 1
top level: BBa_K206001
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K206001/annotation2049257/range2049257/1

https://synbiohub.org/public/igem/BBa_K206001/annotation2049257/range2049257/1
type: Range
persistent identity: range2049257
display id: range2049257
version: 1
direction: direction/forward
top level: BBa_K206001
start: 61
end: 78
orientation: inline

AraI1
type: SequenceAnnotation
persistent identity: annotation2049256
display id: annotation2049256
version: 1
top level: BBa_K206001
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K206001/annotation2049256/range2049256/1

https://synbiohub.org/public/igem/BBa_K206001/annotation2049256/range2049256/1
type: Range
persistent identity: range2049256
display id: range2049256
version: 1
direction: direction/forward
top level: BBa_K206001
start: 40
end: 57
orientation: inline

BBa_K091157
type: ComponentDefinition
persistent identity: BBa K091157
display id: BBa_K091157
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLux/Las Hybrid Promoter
creator: Erin Feeney
created: 2008-07-24T11:00:00Z
modified: 2015-05-08T01:08:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _191_
m user id: 0
owner id: 3067
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: K091157 is a modified version of the pLux' promoter (BBa_K091156) containing the addition of a LasR binding site between the -35 and -10 regions. This promoter is expected to be activated in the presence of AI-1 and LuxR and repressed in the presence of PAI-1 and LasR.
mutable notes: In order to fit the LasR binding site between the -35 and -10 regions, the last base of the Las Box was cropped and the -35 region was modified to overlap with the first two bases of the Las Box.
mutable provenance: The LasR binding site is from the region upstream of the LasI gene in Pseudomonas aeruginosa. The rest of the promoter is the same as BBa_K091156.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: LasR + PAI-1
sequence annotation: -35 Region
sequence annotation: -10 Region
sequence annotation: LuxR+AI-1
sequence: https://synbiohub.org/public/igem/BBa_K091157_sequence/1

LasR + PAI-1
type: SequenceAnnotation
persistent identity: annotation1968425
display id: annotation1968425
version: 1
top level: BBa_K091157
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K091157/annotation1968425/range1968425/1

https://synbiohub.org/public/igem/BBa_K091157/annotation1968425/range1968425/1
type: Range
persistent identity: range1968425
display id: range1968425
version: 1
direction: direction/forward
top level: BBa_K091157
start: 24
end: 42
orientation: inline

-35 Region
type: SequenceAnnotation
persistent identity: annotation1968423
display id: annotation1968423
version: 1
top level: BBa_K091157
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K091157/annotation1968423/range1968423/1

https://synbiohub.org/public/igem/BBa_K091157/annotation1968423/range1968423/1
type: Range
persistent identity: range1968423
display id: range1968423
version: 1
direction: direction/forward
top level: BBa_K091157
start: 20
end: 25
orientation: inline

-10 Region
type: SequenceAnnotation
persistent identity: annotation1968424
display id: annotation1968424
version: 1
top level: BBa_K091157
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K091157/annotation1968424/range1968424/1

https://synbiohub.org/public/igem/BBa_K091157/annotation1968424/range1968424/1
type: Range
persistent identity: range1968424
display id: range1968424
version: 1
direction: direction/forward
top level: BBa_K091157
start: 42
end: 47
orientation: inline

LuxR+AI-1
type: SequenceAnnotation
persistent identity: annotation1968426
display id: annotation1968426
version: 1
top level: BBa_K091157
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K091157/annotation1968426/range1968426/1

https://synbiohub.org/public/igem/BBa_K091157/annotation1968426/range1968426/1
type: Range
persistent identity: range1968426
display id: range1968426
version: 1
direction: direction/forward
top level: BBa_K091157
start: 1
end: 20
orientation: inline

BBa_K338042
type: ComponentDefinition
persistent identity: BBa K338042
display id: BBa_K338042
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP Reporter Device, Heat-Shock Induced
creator: Lucas Hartsough
created: 2010-10-26T11:00:00Z
modified: 2015-05-08T01:12:07Z
discontinued: false
dominant: false
group u list: _454_
m user id: 0
owner id: 6013
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Construct assembled to allow the quantitative analysis of the heat shock promoter, K338001 . Produces GFP in response to low-temperature heat shock.
mutable notes: The type of GFP used in this construct happens to have a very long half life. If growing cultures for fluorescence measurements, grow at 30°C - any higher will activate the promoter, causing GFP to be produced.
mutable provenance: The Registry of Standard Biological Parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0015
component: BBa_K338001
component: BBa_B0034
component: BBa_E0040
sequence annotation: BBa_E0040
sequence annotation: BBa_B0034
sequence annotation: BBa_K338001
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K338042_sequence/1

BBa_B0015
type: Component
persistent identity: component2104994
display id: component2104994
version: 1
top level: BBa_K338042
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K338001
type: Component
persistent identity: component2104983
display id: component2104983
version: 1
top level: BBa_K338042
access: public
definition: HSP

BBa_B0034
type: Component
persistent identity: component2104985
display id: component2104985
version: 1
top level: BBa_K338042
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0040
type: Component
persistent identity: component2104987
display id: component2104987
version: 1
top level: BBa_K338042
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2104987
display id: annotation2104987
version: 1
top level: BBa_K338042
location: https://synbiohub.org/public/igem/BBa_K338042/annotation2104987/range2104987/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K338042/annotation2104987/range2104987/1
type: Range
persistent identity: range2104987
display id: range2104987
version: 1
direction: direction/reverse
top level: BBa_K338042
start: 125
end: 844
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2104985
display id: annotation2104985
version: 1
top level: BBa_K338042
location: https://synbiohub.org/public/igem/BBa_K338042/annotation2104985/range2104985/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K338042/annotation2104985/range2104985/1
type: Range
persistent identity: range2104985
display id: range2104985
version: 1
direction: direction/reverse
top level: BBa_K338042
start: 107
end: 118
orientation: inline

BBa_K338001
type: SequenceAnnotation
persistent identity: annotation2104983
display id: annotation2104983
version: 1
top level: BBa_K338042
location: https://synbiohub.org/public/igem/BBa_K338042/annotation2104983/range2104983/1
component: BBa_K338001

https://synbiohub.org/public/igem/BBa_K338042/annotation2104983/range2104983/1
type: Range
persistent identity: range2104983
display id: range2104983
version: 1
direction: direction/reverse
top level: BBa_K338042
start: 1
end: 98
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2104994
display id: annotation2104994
version: 1
top level: BBa_K338042
location: https://synbiohub.org/public/igem/BBa_K338042/annotation2104994/range2104994/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K338042/annotation2104994/range2104994/1
type: Range
persistent identity: range2104994
display id: range2104994
version: 1
direction: direction/reverse
top level: BBa_K338042
start: 853
end: 981
orientation: inline

BBa_K112406
type: ComponentDefinition
persistent identity: BBa K112406
display id: BBa_K112406
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cadC promoter
creator: Bing Xia
created: 2008-10-21T11:00:00Z
modified: 2015-05-08T01:09:17Z
discontinued: false
dominant: false
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable provenance: Biobricking of cadC promoter and cadC gene PCR cadC-F and cadC-R on MG1655 (2336 bp, EcoR1/BamH1) Sub into pBca1256 (EcoR1/BamH1 910+2472bp, L) Product is pBca1256-K112406 ---------------------------- cb10013-cadC-F cgataGAATTCatgAGATCTcaccagcaatttgcgggcatatc cb10012-cad-R cgtagGGATCCtgaccagaccaaactggcg
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K112406_sequence/1

BBa_K598013
type: ComponentDefinition
persistent identity: BBa K598013
display id: BBa_K598013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBAD+theoHH+CdIntron+E0040+B0015
creator: Xiaowei YAN, Tong MU
created: 2011-09-26T11:00:00Z
modified: 2015-05-08T01:12:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _770_
m user id: 0
owner id: 8713
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This a GFP reporter regulated by an allosteric intron responsive to theophylline.
mutable notes: The ligand response is alter from c-di-GMP to theophylline by merging a theophylline hammerhead ribozyme to the intron.
mutable provenance: The intron derives from the genome of Cd
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: theo HH
sequence annotation: BBa_B0015
sequence annotation: eGFP
sequence annotation: RBS
sequence annotation: pBAD
sequence annotation: group I intron
sequence: https://synbiohub.org/public/igem/BBa_K598013_sequence/1

theo HH
type: SequenceAnnotation
persistent identity: annotation2153072
display id: annotation2153072
version: 1
top level: BBa_K598013
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K598013/annotation2153072/range2153072/1

https://synbiohub.org/public/igem/BBa_K598013/annotation2153072/range2153072/1
type: Range
persistent identity: range2153072
display id: range2153072
version: 1
direction: direction/forward
top level: BBa_K598013
start: 139
end: 210
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2153077
display id: annotation2153077
version: 1
top level: BBa_K598013
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K598013/annotation2153077/range2153077/1

https://synbiohub.org/public/igem/BBa_K598013/annotation2153077/range2153077/1
type: Range
persistent identity: range2153077
display id: range2153077
version: 1
direction: direction/forward
top level: BBa_K598013
start: 1518
end: 1646
orientation: inline

eGFP
type: SequenceAnnotation
persistent identity: annotation2153074
display id: annotation2153074
version: 1
top level: BBa_K598013
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K598013/annotation2153074/range2153074/1

https://synbiohub.org/public/igem/BBa_K598013/annotation2153074/range2153074/1
type: Range
persistent identity: range2153074
display id: range2153074
version: 1
direction: direction/forward
top level: BBa_K598013
start: 790
end: 1509
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2153079
display id: annotation2153079
version: 1
top level: BBa_K598013
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598013/annotation2153079/range2153079/1

https://synbiohub.org/public/igem/BBa_K598013/annotation2153079/range2153079/1
type: Range
persistent identity: range2153079
display id: range2153079
version: 1
direction: direction/forward
top level: BBa_K598013
start: 780
end: 780
orientation: inline

pBAD
type: SequenceAnnotation
persistent identity: annotation2153071
display id: annotation2153071
version: 1
top level: BBa_K598013
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K598013/annotation2153071/range2153071/1

https://synbiohub.org/public/igem/BBa_K598013/annotation2153071/range2153071/1
type: Range
persistent identity: range2153071
display id: range2153071
version: 1
direction: direction/forward
top level: BBa_K598013
start: 1
end: 130
orientation: inline

group I intron
type: SequenceAnnotation
persistent identity: annotation2153073
display id: annotation2153073
version: 1
top level: BBa_K598013
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K598013/annotation2153073/range2153073/1

https://synbiohub.org/public/igem/BBa_K598013/annotation2153073/range2153073/1
type: Range
persistent identity: range2153073
display id: range2153073
version: 1
direction: direction/forward
top level: BBa_K598013
start: 211
end: 789
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2153078
display id: annotation2153078
version: 1
top level: BBa_K598013
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598013/annotation2153078/range2153078/1

https://synbiohub.org/public/igem/BBa_K598013/annotation2153078/range2153078/1
type: Range
persistent identity: range2153078
display id: range2153078
version: 1
direction: direction/forward
top level: BBa_K598013
start: 212
end: 216
orientation: inline

OmpR
type: ComponentDefinition
persistent identity: BBa R0082
display id: BBa_R0082
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (OmpR, positive)
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Positively regulated, OmpR-controlled promoter. This promoter is taken from the upstream region of ompC. Phosphorylated OmpR binds to the three operator sites and activates transcription.
mutable notes: The promoter includes the three OmpR binding sites: C1, C2, and C3. The promoter starts at -107 and goes up to the transcriptional start site at +1. An alternate version, BBa_R0083, cuts out the C2 and C3 sites. The efficacy of this promoter versus the truncated ompC upstream region (BBa_R0083) or the ompF upstream region (BBa_R0084) is currently unknown.
mutable provenance: NC_000193 E. coli K12
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: C2 OmpR
sequence annotation: C3 OmpR
sequence annotation: -10
sequence annotation: -35
sequence annotation: C1 OmpR
sequence: https://synbiohub.org/public/igem/BBa_R0082_sequence/1

C2 OmpR
type: SequenceAnnotation
persistent identity: annotation301155
display id: annotation301155
version: 1
top level: OmpR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301155/range301155/1

https://synbiohub.org/public/igem/BBa_R0082/annotation301155/range301155/1
type: Range
persistent identity: range301155
display id: range301155
version: 1
direction: direction/forward
top level: OmpR
start: 34
end: 51
orientation: inline

C3 OmpR
type: SequenceAnnotation
persistent identity: annotation301156
display id: annotation301156
version: 1
top level: OmpR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301156/range301156/1

https://synbiohub.org/public/igem/BBa_R0082/annotation301156/range301156/1
type: Range
persistent identity: range301156
display id: range301156
version: 1
direction: direction/forward
top level: OmpR
start: 54
end: 71
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation301167
display id: annotation301167
version: 1
top level: OmpR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301167/range301167/1

https://synbiohub.org/public/igem/BBa_R0082/annotation301167/range301167/1
type: Range
persistent identity: range301167
display id: range301167
version: 1
direction: direction/forward
top level: OmpR
start: 98
end: 103
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation301166
display id: annotation301166
version: 1
top level: OmpR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301166/range301166/1

https://synbiohub.org/public/igem/BBa_R0082/annotation301166/range301166/1
type: Range
persistent identity: range301166
display id: range301166
version: 1
direction: direction/forward
top level: OmpR
start: 75
end: 80
orientation: inline

C1 OmpR
type: SequenceAnnotation
persistent identity: annotation301154
display id: annotation301154
version: 1
top level: OmpR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301154/range301154/1

https://synbiohub.org/public/igem/BBa_R0082/annotation301154/range301154/1
type: Range
persistent identity: range301154
display id: range301154
version: 1
direction: direction/forward
top level: OmpR
start: 13
end: 30
orientation: inline

BBa_K110005
type: ComponentDefinition
persistent identity: BBa K110005
display id: BBa_K110005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Alpha-Cell Promoter MF(ALPHA)2
creator: James DiCarlo
created: 2008-05-29T11:00:00Z
modified: 2015-05-08T01:09:08Z
discontinued: false
dominant: false
experience: experience/None
group u list: _201_
m user id: 0
owner id: 2669
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This promoter was isolated by taking 500bp upstream of the MF(ALPHA)2 gene of S. cerevisiae. This promoter will be activated when alpha factor is expressed in an alpha haploid yeast cell.
mutable notes: This gene is on the crick strand of the entered sequence.
mutable provenance: SGD
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: MCM1
sequence annotation: MF(alpha)2
sequence: https://synbiohub.org/public/igem/BBa_K110005_sequence/1

MCM1
type: SequenceAnnotation
persistent identity: annotation1997684
display id: annotation1997684
version: 1
top level: BBa_K110005
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110005/annotation1997684/range1997684/1

https://synbiohub.org/public/igem/BBa_K110005/annotation1997684/range1997684/1
type: Range
persistent identity: range1997684
display id: range1997684
version: 1
direction: direction/forward
top level: BBa_K110005
start: 184
end: 209
orientation: inline

MF(alpha)2
type: SequenceAnnotation
persistent identity: annotation1997686
display id: annotation1997686
version: 1
top level: BBa_K110005
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K110005/annotation1997686/range1997686/1

https://synbiohub.org/public/igem/BBa_K110005/annotation1997686/range1997686/1
type: Range
persistent identity: range1997686
display id: range1997686
version: 1
direction: direction/forward
top level: BBa_K110005
start: 1
end: 500
orientation: inline

BBa_K1700001
type: ComponentDefinition
persistent identity: BBa K1700001
display id: BBa_K1700001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: toehold T3 RNAP
creator: Meixi Liu
created: 2015-09-16T11:00:00Z
modified: 2015-09-17T09:56:37Z
discontinued: false
dominant: false
group u list: _2118_
m user id: 28512
owner id: 28512
owning group id: 9
status: status/Planning
bookmark: false
mutable description: The structure of toehold switch is similar to hairpin, except it has a loop at the top as ???toehold???. Toehold switch functions as riboregulator through linear-linear interaction between RNAs. When target RNA appears, it will bind one of the toehold switch stems and open the loop, exposing the RBS. Toehold switch systems are composed of two RNA strands referred to as the switch and trigger. The switch RNA contains the coding sequence of the gene being regulated. Upstream of this coding sequence is a hairpin-based processing module containing both a strong RBS and a start codon that is followed by a common 21 nt linker sequence coding for low-molecular-weight amino acids added to the N terminus of the gene of interest. A single-stranded toehold sequence at the 50 end of the hairpin module provides the initial binding site for the trigger RNA strand. This trigger molecule contains an extended single-stranded region that completes a branch migration process with the hairpin to expose the RBS and start codon, thereby initiating translation of the gene of interest. Our toehold is upstream of T3 RNAP. The trigger RNA is miRNA-144.
mutable notes: The toehold loop can be opened by trigger RNA, thus controlling the expression of T3 RNAP.
mutable provenance: We sythesis the gene.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: toehold
sequence annotation: T3 RNAP
sequence annotation: ATG start codon
sequence annotation: TAA stop codon
sequence: https://synbiohub.org/public/igem/BBa_K1700001_sequence/1

toehold
type: SequenceAnnotation
persistent identity: annotation2468676
display id: annotation2468676
version: 1
top level: BBa_K1700001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1700001/annotation2468676/range2468676/1

https://synbiohub.org/public/igem/BBa_K1700001/annotation2468676/range2468676/1
type: Range
persistent identity: range2468676
display id: range2468676
version: 1
direction: direction/forward
top level: BBa_K1700001
start: 1
end: 124
orientation: inline

T3 RNAP
type: SequenceAnnotation
persistent identity: annotation2468721
display id: annotation2468721
version: 1
top level: BBa_K1700001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1700001/annotation2468721/range2468721/1

https://synbiohub.org/public/igem/BBa_K1700001/annotation2468721/range2468721/1
type: Range
persistent identity: range2468721
display id: range2468721
version: 1
direction: direction/forward
top level: BBa_K1700001
start: 150
end: 2776
orientation: inline

ATG start codon
type: SequenceAnnotation
persistent identity: annotation2468715
display id: annotation2468715
version: 1
top level: BBa_K1700001
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1700001/annotation2468715/range2468715/1

https://synbiohub.org/public/igem/BBa_K1700001/annotation2468715/range2468715/1
type: Range
persistent identity: range2468715
display id: range2468715
version: 1
direction: direction/forward
top level: BBa_K1700001
start: 150
end: 152
orientation: inline

TAA stop codon
type: SequenceAnnotation
persistent identity: annotation2468722
display id: annotation2468722
version: 1
top level: BBa_K1700001
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1700001/annotation2468722/range2468722/1

https://synbiohub.org/public/igem/BBa_K1700001/annotation2468722/range2468722/1
type: Range
persistent identity: range2468722
display id: range2468722
version: 1
direction: direction/forward
top level: BBa_K1700001
start: 2774
end: 2776
orientation: inline

las TetO
type: ComponentDefinition
persistent identity: BBa I14015
display id: BBa_I14015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: P(Las) TetO
creator: Vikram Vijayan, Allen Hsu, Lawrence Fomundam
created: 2004-07-29T11:00:00Z
modified: 2015-08-31T04:07:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _4_
m user id: 0
owner id: 171
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: LasR and 3OC12HSL regulated promoter with a single Tet Operator (binding site for TetR repressor)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: -10
sequence annotation: TetR
sequence annotation: R0079
sequence: https://synbiohub.org/public/igem/BBa_I14015_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation1026120
display id: annotation1026120
version: 1
top level: las TetO
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I14015/annotation1026120/range1026120/1

https://synbiohub.org/public/igem/BBa_I14015/annotation1026120/range1026120/1
type: Range
persistent identity: range1026120
display id: range1026120
version: 1
direction: direction/forward
top level: las TetO
start: 117
end: 122
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1026116
display id: annotation1026116
version: 1
top level: las TetO
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I14015/annotation1026116/range1026116/1

https://synbiohub.org/public/igem/BBa_I14015/annotation1026116/range1026116/1
type: Range
persistent identity: range1026116
display id: range1026116
version: 1
direction: direction/forward
top level: las TetO
start: 140
end: 145
orientation: inline

TetR
type: SequenceAnnotation
persistent identity: annotation1017910
display id: annotation1017910
version: 1
top level: las TetO
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I14015/annotation1017910/range1017910/1

https://synbiohub.org/public/igem/BBa_I14015/annotation1017910/range1017910/1
type: Range
persistent identity: range1017910
display id: range1017910
version: 1
direction: direction/forward
top level: las TetO
start: 152
end: 170
orientation: inline

R0079
type: SequenceAnnotation
persistent identity: annotation1017909
display id: annotation1017909
version: 1
top level: las TetO
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I14015/annotation1017909/range1017909/1

https://synbiohub.org/public/igem/BBa_I14015/annotation1017909/range1017909/1
type: Range
persistent identity: range1017909
display id: range1017909
version: 1
direction: direction/forward
top level: las TetO
start: 1
end: 151
orientation: inline

BBa_J61054
type: ComponentDefinition
persistent identity: BBa J61054
display id: BBa_J61054
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [HIP-1] Promoter
creator: John Anderson
created: 2007-02-20T12:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: non
mutable notes: non
mutable provenance: non
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J61054_sequence/1

BBa_K606039
type: ComponentDefinition
persistent identity: BBa K606039
display id: BBa_K606039
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pVeg SpoVG T7 SpoVG RFP double terminator.
creator: Axel S??guret
created: 2011-09-17T11:00:00Z
modified: 2015-05-08T01:12:51Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _778_
m user id: 0
owner id: 8931
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: T7 and RFP expression system in B. subtilis
mutable notes: biobrick assembly
mutable provenance: B. subtilis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K145001
component: BBa_K143053
component: BBa_K606013
sequence annotation: BBa_K606013
sequence annotation: BBa_K143053
sequence annotation: BBa_K145001
sequence: https://synbiohub.org/public/igem/BBa_K606039_sequence/1

BBa_K145001
type: Component
persistent identity: component2268491
display id: component2268491
version: 1
top level: BBa_K606039
access: public
definition: https://synbiohub.org/public/igem/BBa_K145001/1

BBa_K143053
type: Component
persistent identity: component2268486
display id: component2268486
version: 1
top level: BBa_K606039
access: public
definition: https://synbiohub.org/public/igem/BBa_K143053/1

BBa_K606013
type: Component
persistent identity: component2268505
display id: component2268505
version: 1
top level: BBa_K606039
access: public
definition: https://synbiohub.org/public/igem/BBa_K606013/1

BBa_K606013
type: SequenceAnnotation
persistent identity: annotation2268505
display id: annotation2268505
version: 1
top level: BBa_K606039
location: https://synbiohub.org/public/igem/BBa_K606039/annotation2268505/range2268505/1
component: BBa_K606013

https://synbiohub.org/public/igem/BBa_K606039/annotation2268505/range2268505/1
type: Range
persistent identity: range2268505
display id: range2268505
version: 1
direction: direction/reverse
top level: BBa_K606039
start: 2787
end: 3647
orientation: inline

BBa_K143053
type: SequenceAnnotation
persistent identity: annotation2268486
display id: annotation2268486
version: 1
top level: BBa_K606039
location: https://synbiohub.org/public/igem/BBa_K606039/annotation2268486/range2268486/1
component: BBa_K143053

https://synbiohub.org/public/igem/BBa_K606039/annotation2268486/range2268486/1
type: Range
persistent identity: range2268486
display id: range2268486
version: 1
direction: direction/reverse
top level: BBa_K606039
start: 1
end: 117
orientation: inline

BBa_K145001
type: SequenceAnnotation
persistent identity: annotation2268491
display id: annotation2268491
version: 1
top level: BBa_K606039
location: https://synbiohub.org/public/igem/BBa_K606039/annotation2268491/range2268491/1
component: BBa_K145001

https://synbiohub.org/public/igem/BBa_K606039/annotation2268491/range2268491/1
type: Range
persistent identity: range2268491
display id: range2268491
version: 1
direction: direction/reverse
top level: BBa_K606039
start: 124
end: 2778
orientation: inline

Las CIO
type: ComponentDefinition
persistent identity: BBa I14016
display id: BBa_I14016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: P(Las) CIO
creator: Vikram Vijayan, Allen Hsu, Lawrence Fomundam
created: 2004-07-29T11:00:00Z
modified: 2015-08-31T04:07:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _4_
m user id: 0
owner id: 171
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: LasR and 3OC12HSL regulated promoter with CI Operator 1 (binding site for lambda cI repressor)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: CI lambda O1
sequence annotation: -10
sequence annotation: R0079
sequence: https://synbiohub.org/public/igem/BBa_I14016_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation1026124
display id: annotation1026124
version: 1
top level: Las CIO
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I14016/annotation1026124/range1026124/1

https://synbiohub.org/public/igem/BBa_I14016/annotation1026124/range1026124/1
type: Range
persistent identity: range1026124
display id: range1026124
version: 1
direction: direction/forward
top level: Las CIO
start: 117
end: 122
orientation: inline

CI lambda O1
type: SequenceAnnotation
persistent identity: annotation1017878
display id: annotation1017878
version: 1
top level: Las CIO
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I14016/annotation1017878/range1017878/1

https://synbiohub.org/public/igem/BBa_I14016/annotation1017878/range1017878/1
type: Range
persistent identity: range1017878
display id: range1017878
version: 1
direction: direction/forward
top level: Las CIO
start: 152
end: 168
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1026125
display id: annotation1026125
version: 1
top level: Las CIO
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I14016/annotation1026125/range1026125/1

https://synbiohub.org/public/igem/BBa_I14016/annotation1026125/range1026125/1
type: Range
persistent identity: range1026125
display id: range1026125
version: 1
direction: direction/forward
top level: Las CIO
start: 140
end: 145
orientation: inline

R0079
type: SequenceAnnotation
persistent identity: annotation1017859
display id: annotation1017859
version: 1
top level: Las CIO
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I14016/annotation1017859/range1017859/1

https://synbiohub.org/public/igem/BBa_I14016/annotation1017859/range1017859/1
type: Range
persistent identity: range1017859
display id: range1017859
version: 1
direction: direction/forward
top level: Las CIO
start: 1
end: 151
orientation: inline

MarA
type: ComponentDefinition
persistent identity: BBa K1230000
display id: BBa_K1230000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AcrAB-TolC multidrug efflux transport system activator from E. coli
creator: Andr??s Ferri??o Iriarte
created: 2013-09-07T11:00:00Z
modified: 2015-05-08T01:09:44Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1544_
m user id: 0
owner id: 17080
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The MarA protein acts as a transcriptional activator in E. coli, it allows the expression of the acrAB and tolC operons, which activate the AcrAB-TolC efflux pump, a mechanism that has been related with resistance to organic solvents, dyes, detergents, antibiotics such as chloramphenicol, tetracycline, novobiocin, erythromycin, fusidic acid and cloxacillin, as well as to cationic antimicrobial peptides, such as LL-37, HNP-2 and HBD-1.
mutable notes: Silent mutation (G->C) at base 132 to remove AarI and BspMI sites.
mutable provenance: Synthesis from genscript
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: G to C mutation
sequence annotation: stop
sequence annotation: MarA
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K1230000_sequence/1

G to C mutation
type: SequenceAnnotation
persistent identity: annotation2335368
display id: annotation2335368
version: 1
top level: MarA
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K1230000/annotation2335368/range2335368/1

https://synbiohub.org/public/igem/BBa_K1230000/annotation2335368/range2335368/1
type: Range
persistent identity: range2335368
display id: range2335368
version: 1
direction: direction/forward
top level: MarA
start: 132
end: 132
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2335366
display id: annotation2335366
version: 1
top level: MarA
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1230000/annotation2335366/range2335366/1

https://synbiohub.org/public/igem/BBa_K1230000/annotation2335366/range2335366/1
type: Range
persistent identity: range2335366
display id: range2335366
version: 1
direction: direction/forward
top level: MarA
start: 388
end: 393
orientation: inline

MarA
type: SequenceAnnotation
persistent identity: annotation2335365
display id: annotation2335365
version: 1
top level: MarA
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1230000/annotation2335365/range2335365/1

https://synbiohub.org/public/igem/BBa_K1230000/annotation2335365/range2335365/1
type: Range
persistent identity: range2335365
display id: range2335365
version: 1
direction: direction/forward
top level: MarA
start: 1
end: 393
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2335367
display id: annotation2335367
version: 1
top level: MarA
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1230000/annotation2335367/range2335367/1

https://synbiohub.org/public/igem/BBa_K1230000/annotation2335367/range2335367/1
type: Range
persistent identity: range2335367
display id: range2335367
version: 1
direction: direction/forward
top level: MarA
start: 1
end: 3
orientation: inline

BBa_K112149
type: ComponentDefinition
persistent identity: BBa K112149
display id: BBa_K112149
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PmgtCB Magnesium promoter from Salmonella
creator: Madhvi Venkatesh
created: 2008-11-01T12:00:00Z
modified: 2015-05-08T01:09:14Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 3023
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: PmgtCB magnesium promoter
mutable notes: This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: BBb Standard Description Page
mutable provenance: genome
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K112149_sequence/1

BBa_K284008
type: ComponentDefinition
persistent identity: BBa K284008
display id: BBa_K284008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tra operon promoter (Py)
creator: Fabiana de Melo Duarte, Leonardo Minete, Maria Carolina Grassi and Lucas P. Parizzi
created: 2009-08-05T11:00:00Z
modified: 2015-05-08T01:11:48Z
discontinued: false
dominant: false
experience: experience/None
group u list: _386_
m user id: 0
owner id: 4966
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Py is the tra operon promoter of F-plasmid. It is repressed by the host nucleoid-associated protein, H-NS and activeted by TraJ protein. Conflicting evidence suggests that TraY can act as both an activator and a repressor. The tra operon encodes F-plasmid proteins (except TraJ and TraM) involved in DNA transfer by conjugation in E.coli. The regions shows intrinsic curvature, which could interfere in regulation. For this reason, the two closer bends upstream promoter region are included.
mutable notes: The mechanism for controlling transcription from Py remains undefined. The Py region contains significant intrinsic curvature, which could interfere in regulation
mutable provenance: F-plasmid of Escherichia coli.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: bend
sequence annotation: -35
sequence annotation: Tray binding site
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_K284008_sequence/1

bend
type: SequenceAnnotation
persistent identity: annotation2015153
display id: annotation2015153
version: 1
top level: BBa_K284008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K284008/annotation2015153/range2015153/1

https://synbiohub.org/public/igem/BBa_K284008/annotation2015153/range2015153/1
type: Range
persistent identity: range2015153
display id: range2015153
version: 1
direction: direction/forward
top level: BBa_K284008
start: 17
end: 44
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2015150
display id: annotation2015150
version: 1
top level: BBa_K284008
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K284008/annotation2015150/range2015150/1

https://synbiohub.org/public/igem/BBa_K284008/annotation2015150/range2015150/1
type: Range
persistent identity: range2015150
display id: range2015150
version: 1
direction: direction/forward
top level: BBa_K284008
start: 69
end: 74
orientation: inline

Tray binding site
type: SequenceAnnotation
persistent identity: annotation2015149
display id: annotation2015149
version: 1
top level: BBa_K284008
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K284008/annotation2015149/range2015149/1

https://synbiohub.org/public/igem/BBa_K284008/annotation2015149/range2015149/1
type: Range
persistent identity: range2015149
display id: range2015149
version: 1
direction: direction/forward
top level: BBa_K284008
start: 108
end: 133
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2015151
display id: annotation2015151
version: 1
top level: BBa_K284008
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K284008/annotation2015151/range2015151/1

https://synbiohub.org/public/igem/BBa_K284008/annotation2015151/range2015151/1
type: Range
persistent identity: range2015151
display id: range2015151
version: 1
direction: direction/forward
top level: BBa_K284008
start: 92
end: 97
orientation: inline

bend
type: SequenceAnnotation
persistent identity: annotation2015152
display id: annotation2015152
version: 1
top level: BBa_K284008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K284008/annotation2015152/range2015152/1

https://synbiohub.org/public/igem/BBa_K284008/annotation2015152/range2015152/1
type: Range
persistent identity: range2015152
display id: range2015152
version: 1
direction: direction/forward
top level: BBa_K284008
start: 47
end: 68
orientation: inline

BBa_K190017
type: ComponentDefinition
persistent identity: BBa K190017
display id: BBa_K190017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Copper Promoter (CueR regulated)
creator: Michael Verhoeven
created: 2009-07-14T11:00:00Z
modified: 2015-05-08T01:11:15Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _306_
m user id: 0
owner id: 4144
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Promoter sequence with recognition site for CueR transcriptional regulator protein.
mutable notes: None
mutable provenance: E.coli TOP10
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35 and -10 region
sequence annotation: CueR binding site
sequence: https://synbiohub.org/public/igem/BBa_K190017_sequence/1

-35 and -10 region
type: SequenceAnnotation
persistent identity: annotation2011505
display id: annotation2011505
version: 1
top level: BBa_K190017
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K190017/annotation2011505/range2011505/1

https://synbiohub.org/public/igem/BBa_K190017/annotation2011505/range2011505/1
type: Range
persistent identity: range2011505
display id: range2011505
version: 1
direction: direction/forward
top level: BBa_K190017
start: 1
end: 43
orientation: inline

CueR binding site
type: SequenceAnnotation
persistent identity: annotation2011506
display id: annotation2011506
version: 1
top level: BBa_K190017
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K190017/annotation2011506/range2011506/1

https://synbiohub.org/public/igem/BBa_K190017/annotation2011506/range2011506/1
type: Range
persistent identity: range2011506
display id: range2011506
version: 1
direction: direction/forward
top level: BBa_K190017
start: 3
end: 28
orientation: inline

BBa_K180019
type: ComponentDefinition
persistent identity: BBa K180019
display id: BBa_K180019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Scissors - Primary plasmid (part 2) [tac pR, mBanana and LuxI generator]
creator: Richard Wilson, Stephanie Chambers
created: 2009-10-18T11:00:00Z
modified: 2015-05-08T01:11:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _278_
m user id: 0
owner id: 4082
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Part of the iGEM2009 Team:Southampton rock-paper-scissors 'scissors' primary plasmid.
mutable notes: Regulated expression of LasI (C0178) and mBanana (K180009) by tac pR (K180000), controlled by presence of lac/IPTG or trp in the cell.
mutable provenance: See relevant basic biobrick parts (K180000, B0034, K180009, B0015).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K180009
component: BBa_B0010
component: BBa_B0034
component: BBa_K180000
component: BBa_B0012
component: BBa_C0161
sequence annotation: BBa_C0161
sequence annotation: BBa_K180009
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_K180000
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_K180019_sequence/1

BBa_K180009
type: Component
persistent identity: component2058405
display id: component2058405
version: 1
top level: BBa_K180019
access: public
definition: https://synbiohub.org/public/igem/BBa_K180009/1

BBa_B0010
type: Component
persistent identity: component2058410
display id: component2058410
version: 1
top level: BBa_K180019
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component2058403
display id: component2058403
version: 1
top level: BBa_K180019
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: Component
persistent identity: component2058407
display id: component2058407
version: 1
top level: BBa_K180019
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K180000
type: Component
persistent identity: component2058401
display id: component2058401
version: 1
top level: BBa_K180019
access: public
definition: BBa_K180000

BBa_B0012
type: Component
persistent identity: component2058412
display id: component2058412
version: 1
top level: BBa_K180019
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0161
type: Component
persistent identity: component2058409
display id: component2058409
version: 1
top level: BBa_K180019
access: public
definition: https://synbiohub.org/public/igem/BBa_C0161/1

BBa_C0161
type: SequenceAnnotation
persistent identity: annotation2058409
display id: annotation2058409
version: 1
top level: BBa_K180019
location: https://synbiohub.org/public/igem/BBa_K180019/annotation2058409/range2058409/1
component: BBa_C0161

https://synbiohub.org/public/igem/BBa_K180019/annotation2058409/range2058409/1
type: Range
persistent identity: range2058409
display id: range2058409
version: 1
direction: direction/reverse
top level: BBa_K180019
start: 2208
end: 2792
orientation: inline

BBa_K180009
type: SequenceAnnotation
persistent identity: annotation2058405
display id: annotation2058405
version: 1
top level: BBa_K180019
location: https://synbiohub.org/public/igem/BBa_K180019/annotation2058405/range2058405/1
component: BBa_K180009

https://synbiohub.org/public/igem/BBa_K180019/annotation2058405/range2058405/1
type: Range
persistent identity: range2058405
display id: range2058405
version: 1
direction: direction/reverse
top level: BBa_K180019
start: 1474
end: 2181
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2058403
display id: annotation2058403
version: 1
top level: BBa_K180019
location: https://synbiohub.org/public/igem/BBa_K180019/annotation2058403/range2058403/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K180019/annotation2058403/range2058403/1
type: Range
persistent identity: range2058403
display id: range2058403
version: 1
direction: direction/reverse
top level: BBa_K180019
start: 1456
end: 1467
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2058407
display id: annotation2058407
version: 1
top level: BBa_K180019
location: https://synbiohub.org/public/igem/BBa_K180019/annotation2058407/range2058407/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K180019/annotation2058407/range2058407/1
type: Range
persistent identity: range2058407
display id: range2058407
version: 1
direction: direction/reverse
top level: BBa_K180019
start: 2190
end: 2201
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2058412
display id: annotation2058412
version: 1
top level: BBa_K180019
location: https://synbiohub.org/public/igem/BBa_K180019/annotation2058412/range2058412/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K180019/annotation2058412/range2058412/1
type: Range
persistent identity: range2058412
display id: range2058412
version: 1
direction: direction/forward
top level: BBa_K180019
start: 2889
end: 2929
orientation: inline

BBa_K180000
type: SequenceAnnotation
persistent identity: annotation2058401
display id: annotation2058401
version: 1
top level: BBa_K180019
location: https://synbiohub.org/public/igem/BBa_K180019/annotation2058401/range2058401/1
component: BBa_K180000

https://synbiohub.org/public/igem/BBa_K180019/annotation2058401/range2058401/1
type: Range
persistent identity: range2058401
display id: range2058401
version: 1
direction: direction/reverse
top level: BBa_K180019
start: 1
end: 1447
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2058410
display id: annotation2058410
version: 1
top level: BBa_K180019
location: https://synbiohub.org/public/igem/BBa_K180019/annotation2058410/range2058410/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K180019/annotation2058410/range2058410/1
type: Range
persistent identity: range2058410
display id: range2058410
version: 1
direction: direction/forward
top level: BBa_K180019
start: 2801
end: 2880
orientation: inline

BBa_K180005
type: ComponentDefinition
persistent identity: BBa K180005
display id: BBa_K180005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GoL - Primary plasmid (part 1)/RPS - Paper primary plasmid (part 1) [LuxR generator]
creator: Richard Wilson, Stephanie Chambers
created: 2009-10-15T11:00:00Z
modified: 2015-05-08T01:11:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _278_
m user id: 0
owner id: 4082
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Part of the iGEM2009 Team:Southampton Game of Life primary plasmid.
mutable notes: Constitutive promoter selected to allow for continuous expression of C0062.
mutable provenance: See relevant basic biobrick parts.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_C0062
component: BBa_B0015
component: BBa_J23119
sequence annotation: BBa_B0034
sequence annotation: BBa_C0062
sequence annotation: BBa_J23119
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K180005_sequence/1

BBa_B0034
type: Component
persistent identity: component2255826
display id: component2255826
version: 1
top level: BBa_K180005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0062
type: Component
persistent identity: component2255829
display id: component2255829
version: 1
top level: BBa_K180005
access: public
definition: https://synbiohub.org/public/igem/BBa_C0062/1

BBa_B0015
type: Component
persistent identity: component2255839
display id: component2255839
version: 1
top level: BBa_K180005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_J23119
type: Component
persistent identity: component2255824
display id: component2255824
version: 1
top level: BBa_K180005
access: public
definition: https://synbiohub.org/public/igem/BBa_J23119/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2255826
display id: annotation2255826
version: 1
top level: BBa_K180005
location: https://synbiohub.org/public/igem/BBa_K180005/annotation2255826/range2255826/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K180005/annotation2255826/range2255826/1
type: Range
persistent identity: range2255826
display id: range2255826
version: 1
direction: direction/reverse
top level: BBa_K180005
start: 44
end: 55
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation2255829
display id: annotation2255829
version: 1
top level: BBa_K180005
location: https://synbiohub.org/public/igem/BBa_K180005/annotation2255829/range2255829/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_K180005/annotation2255829/range2255829/1
type: Range
persistent identity: range2255829
display id: range2255829
version: 1
direction: direction/forward
top level: BBa_K180005
start: 62
end: 817
orientation: inline

BBa_J23119
type: SequenceAnnotation
persistent identity: annotation2255824
display id: annotation2255824
version: 1
top level: BBa_K180005
location: https://synbiohub.org/public/igem/BBa_K180005/annotation2255824/range2255824/1
component: BBa_J23119

https://synbiohub.org/public/igem/BBa_K180005/annotation2255824/range2255824/1
type: Range
persistent identity: range2255824
display id: range2255824
version: 1
direction: direction/reverse
top level: BBa_K180005
start: 1
end: 35
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2255839
display id: annotation2255839
version: 1
top level: BBa_K180005
location: https://synbiohub.org/public/igem/BBa_K180005/annotation2255839/range2255839/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K180005/annotation2255839/range2255839/1
type: Range
persistent identity: range2255839
display id: range2255839
version: 1
direction: direction/reverse
top level: BBa_K180005
start: 851
end: 979
orientation: inline

BBa_J64804
type: ComponentDefinition
persistent identity: BBa J64804
display id: BBa_J64804
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: The promoter region (inclusive of regulator binding sites) of the B. subtilis RocDEF operon
creator: Sai Duriseti
created: 2007-03-19T12:00:00Z
modified: 2015-05-08T01:08:17Z
discontinued: false
dominant: false
group u list: _98_
m user id: 0
owner id: 1428
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the reported promoter region from the Bacillus subtilis RocDEF operon as taken from the DBTBS website (http://dbtbs.hgc.jp/). Base 1 to base 21 contains the binding site for the binding factor, RocR. The sequence, TATGCAAAAGAATTTTGCACT, reportedly contains the consensus sequence for binding of this factor. Base 42 to base 62 contains another binding site for the RocR binding factor, with ATATCAGAATGTTTTTGCACC as the binding consensus for this sequence. Bases 113-135 outline the binding site of the binding factor AhrC (CTTGCATTTATATAAAGGGAAAG). Bases 96 to 135 outline the promoter region for this operon (CTTGATTTGGCACAGAACTTGCATTTATATAAAGGGAAAG).
mutable notes: This sequence only outlines the reported promoter region as ascertained from the online resource described above, and may lack nucleotides not described by the operon analysis used.
mutable provenance: Information was obtained from: http://dbtbs.hgc.jp/COG/prom/rocDEF.html.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: RocR Binding Site
sequence annotation: Promoter
sequence annotation: AhrC
sequence: https://synbiohub.org/public/igem/BBa_J64804_sequence/1

RocR Binding Site
type: SequenceAnnotation
persistent identity: annotation1920952
display id: annotation1920952
version: 1
top level: BBa_J64804
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J64804/annotation1920952/range1920952/1

https://synbiohub.org/public/igem/BBa_J64804/annotation1920952/range1920952/1
type: Range
persistent identity: range1920952
display id: range1920952
version: 1
direction: direction/forward
top level: BBa_J64804
start: 42
end: 62
orientation: inline

Promoter
type: SequenceAnnotation
persistent identity: annotation1920953
display id: annotation1920953
version: 1
top level: BBa_J64804
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J64804/annotation1920953/range1920953/1

https://synbiohub.org/public/igem/BBa_J64804/annotation1920953/range1920953/1
type: Range
persistent identity: range1920953
display id: range1920953
version: 1
direction: direction/forward
top level: BBa_J64804
start: 96
end: 135
orientation: inline

AhrC
type: SequenceAnnotation
persistent identity: annotation1920955
display id: annotation1920955
version: 1
top level: BBa_J64804
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J64804/annotation1920955/range1920955/1

https://synbiohub.org/public/igem/BBa_J64804/annotation1920955/range1920955/1
type: Range
persistent identity: range1920955
display id: range1920955
version: 1
direction: direction/forward
top level: BBa_J64804
start: 113
end: 131
orientation: inline

RocR Binding Site
type: SequenceAnnotation
persistent identity: annotation1920951
display id: annotation1920951
version: 1
top level: BBa_J64804
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J64804/annotation1920951/range1920951/1

https://synbiohub.org/public/igem/BBa_J64804/annotation1920951/range1920951/1
type: Range
persistent identity: range1920951
display id: range1920951
version: 1
direction: direction/forward
top level: BBa_J64804
start: 1
end: 21
orientation: inline

BBa_K112322
type: ComponentDefinition
persistent identity: BBa K112322
display id: BBa_K112322
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {Pdps} in BBb format
creator: Aron Lau
created: 2008-10-21T11:00:00Z
modified: 2015-05-08T01:09:16Z
discontinued: false
dominant: false
group u list: _224_
m user id: 0
owner id: 2999
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Pdps promoter. This part is actually in BBb format. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: PCR JH089F/JH089R on Salmonella L2. (379 bp, EcoRI/BamHI/DpnI) Sub into pBjh1601CK (EcoRI/BamHI, 3134+910, L) Product is pBjh1601CK-K112322 ----------------------------------------------- JH089F Forward biobricking of {Pdps} cgataGAATTCatgAGATCTaacgattcgacgcgaaccgc JH089R Reverse biobricking of {Pdps} cgttaGGATCCaatctcatatcctcttgatg
mutable provenance: Salmonella L2
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K112322_sequence/1

BBa_K1486046
type: ComponentDefinition
persistent identity: BBa K1486046
display id: BBa_K1486046
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CpxR promoter FW
creator: EPFL iGem team 2014
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21033
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Test
mutable notes: Test
mutable provenance: Test
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1486046_sequence/1

BBa_I746364
type: ComponentDefinition
persistent identity: BBa I746364
display id: BBa_I746364
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Psid promoter from P4 phage
creator: Stefan Milde
created: 2007-09-10T11:00:00Z
modified: 2015-08-31T04:08:04Z
discontinued: false
dominant: false
group u list: _116_
m user id: 0
owner id: 2122
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the Psid promoter taken from the P4 phage genome. It is an inducible promoter that is activated by a class of activators, including P2 ogr (I746350), PSP3 pag (I746351) and phiR73 delta (I746352). These different activators should cause different levels of activity of the Psid promoter.
mutable notes: no special considerations
mutable provenance: P4 phage genome
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Psid
sequence: https://synbiohub.org/public/igem/BBa_I746364_sequence/1

Psid
type: SequenceAnnotation
persistent identity: annotation1943787
display id: annotation1943787
version: 1
top level: BBa_I746364
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I746364/annotation1943787/range1943787/1

https://synbiohub.org/public/igem/BBa_I746364/annotation1943787/range1943787/1
type: Range
persistent identity: range1943787
display id: range1943787
version: 1
direction: direction/forward
top level: BBa_I746364
start: 1
end: 93
orientation: inline

BBa_K389413
type: ComponentDefinition
persistent identity: BBa K389413
display id: BBa_K389413
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: vir promoter + sensitivity tuner + luciferase
creator: Jonas Aretz
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part combines a mutated virG response regulator from the VirA/G receptor system under the control of a constitutive promoter with a sensitivity tuner before a luciferase gene under the control of a vir promoter. The sensitivity tuner enhances a signal from a promoter so it enhances the readout luciferase.
mutable notes: sensitivity tuner to enhance the luciferase signal
mutable provenance: * luciferase from pGL4.10luc2 (Promega) * sensitivity tuner part, terminators and constitutive promoter from the partsregistry * ''virG'' was synthesized by Mr. Gene * ''vir'' promoter from Agrobacterium tumefaciens C58, TI-plasmid
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K389403
component: BBa_B0017
component: BBa_J23110
component: BBa_K389003
component: BBa_K389002
sequence annotation: BBa_K389003
sequence annotation: BBa_K389403
sequence annotation: BBa_K389002
sequence annotation: BBa_B0017
sequence annotation: BBa_J23110
sequence: https://synbiohub.org/public/igem/BBa_K389413_sequence/1

BBa_K389403
type: Component
persistent identity: component2300839
display id: component2300839
version: 1
top level: BBa_K389413
access: public
definition: https://synbiohub.org/public/igem/BBa_K389403/1

BBa_B0017
type: Component
persistent identity: component2300809
display id: component2300809
version: 1
top level: BBa_K389413
access: public
definition: https://synbiohub.org/public/igem/BBa_B0017/1

BBa_J23110
type: Component
persistent identity: component2300800
display id: component2300800
version: 1
top level: BBa_K389413
access: public
definition: https://synbiohub.org/public/igem/BBa_J23110/1

BBa_K389003
type: Component
persistent identity: component2300814
display id: component2300814
version: 1
top level: BBa_K389413
access: public
definition: Pvir

BBa_K389002
type: Component
persistent identity: component2300804
display id: component2300804
version: 1
top level: BBa_K389413
access: public
definition: https://synbiohub.org/public/igem/BBa_K389002/1

BBa_K389003
type: SequenceAnnotation
persistent identity: annotation2300814
display id: annotation2300814
version: 1
top level: BBa_K389413
location: https://synbiohub.org/public/igem/BBa_K389413/annotation2300814/range2300814/1
component: BBa_K389003

https://synbiohub.org/public/igem/BBa_K389413/annotation2300814/range2300814/1
type: Range
persistent identity: range2300814
display id: range2300814
version: 1
direction: direction/reverse
top level: BBa_K389413
start: 968
end: 1125
orientation: inline

BBa_K389403
type: SequenceAnnotation
persistent identity: annotation2300839
display id: annotation2300839
version: 1
top level: BBa_K389413
location: https://synbiohub.org/public/igem/BBa_K389413/annotation2300839/range2300839/1
component: BBa_K389403

https://synbiohub.org/public/igem/BBa_K389413/annotation2300839/range2300839/1
type: Range
persistent identity: range2300839
display id: range2300839
version: 1
direction: direction/reverse
top level: BBa_K389413
start: 1134
end: 4047
orientation: inline

BBa_K389002
type: SequenceAnnotation
persistent identity: annotation2300804
display id: annotation2300804
version: 1
top level: BBa_K389413
location: https://synbiohub.org/public/igem/BBa_K389413/annotation2300804/range2300804/1
component: BBa_K389002

https://synbiohub.org/public/igem/BBa_K389413/annotation2300804/range2300804/1
type: Range
persistent identity: range2300804
display id: range2300804
version: 1
direction: direction/reverse
top level: BBa_K389413
start: 44
end: 783
orientation: inline

BBa_B0017
type: SequenceAnnotation
persistent identity: annotation2300809
display id: annotation2300809
version: 1
top level: BBa_K389413
location: https://synbiohub.org/public/igem/BBa_K389413/annotation2300809/range2300809/1
component: BBa_B0017

https://synbiohub.org/public/igem/BBa_K389413/annotation2300809/range2300809/1
type: Range
persistent identity: range2300809
display id: range2300809
version: 1
direction: direction/reverse
top level: BBa_K389413
start: 792
end: 959
orientation: inline

BBa_J23110
type: SequenceAnnotation
persistent identity: annotation2300800
display id: annotation2300800
version: 1
top level: BBa_K389413
location: https://synbiohub.org/public/igem/BBa_K389413/annotation2300800/range2300800/1
component: BBa_J23110

https://synbiohub.org/public/igem/BBa_K389413/annotation2300800/range2300800/1
type: Range
persistent identity: range2300800
display id: range2300800
version: 1
direction: direction/reverse
top level: BBa_K389413
start: 1
end: 35
orientation: inline

BBa_I746363
type: ComponentDefinition
persistent identity: BBa I746363
display id: BBa_I746363
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PV promoter from P2 phage
creator: Stefan Milde
created: 2007-09-10T11:00:00Z
modified: 2015-08-31T04:08:04Z
discontinued: false
dominant: false
group u list: _116_
m user id: 0
owner id: 2122
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the PF promoter taken from the P2 phage genome. It is an inducible promoter that is activated by a class of activators, including P2 ogr (I746350), PSP3 pag (I746351) and phiR73 delta (I746352). These different activators should cause different levels of activity of the PF promoter.
mutable notes: no special considerations
mutable provenance: P2 phage genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: PV
sequence: https://synbiohub.org/public/igem/BBa_I746363_sequence/1

PV
type: SequenceAnnotation
persistent identity: annotation1943786
display id: annotation1943786
version: 1
top level: BBa_I746363
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I746363/annotation1943786/range1943786/1

https://synbiohub.org/public/igem/BBa_I746363/annotation1943786/range1943786/1
type: Range
persistent identity: range1943786
display id: range1943786
version: 1
direction: direction/forward
top level: BBa_I746363
start: 1
end: 91
orientation: inline

BBa_I746362
type: ComponentDefinition
persistent identity: BBa I746362
display id: BBa_I746362
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PP promoter from P2 phage
creator: Stefan Milde
created: 2007-09-10T11:00:00Z
modified: 2015-08-31T04:08:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _116_
m user id: 0
owner id: 2122
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is the PP promoter taken from the P2 phage genome. It is an inducible promoter that is activated by a class of activators, including P2 ogr (I746350), PSP3 pag (I746351) and phiR73 delta (I746352). These different activators should cause different levels of activity of the PP promoter.
mutable notes: no special considerations
mutable provenance: P2 phage genome
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: PP
sequence: https://synbiohub.org/public/igem/BBa_I746362_sequence/1

PP
type: SequenceAnnotation
persistent identity: annotation1943785
display id: annotation1943785
version: 1
top level: BBa_I746362
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I746362/annotation1943785/range1943785/1

https://synbiohub.org/public/igem/BBa_I746362/annotation1943785/range1943785/1
type: Range
persistent identity: range1943785
display id: range1943785
version: 1
direction: direction/forward
top level: BBa_I746362
start: 1
end: 92
orientation: inline

Lock 1
type: ComponentDefinition
persistent identity: BBa J01010
display id: BBa_J01010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Riboregulator Lock 1
creator: Golden Bear
created: 2005-11-05T12:00:00Z
modified: 2015-08-31T04:08:12Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _13_
m user id: 0
owner id: 395
owning group id: 13
sample status: In stock
status: status/Available
bookmark: false
mutable description: Biobricked version of Isaacs' riboregulator cis repressed lock, crR12.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence: https://synbiohub.org/public/igem/BBa_J01010_sequence/1

BBa_K658008
type: ComponentDefinition
persistent identity: BBa K658008
display id: BBa_K658008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: position 3&5 mutated promoter lux pR-3/5 (luxR & HSL regulated)
creator: Yidan Hu
created: 2011-09-30T11:00:00Z
modified: 2015-05-08T01:13:02Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _835_
m user id: 0
owner id: 10207
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The position 3&5 mutated promoter lux pR-3/5 is a product of site-directed mutagenesis of promoter lux pR (BBa_R0062). The promoter lux pR is based on the Vibrio fischeri quorum sensing gene promoters. The position 5 mutated promoter lux pR-3/5 gives a lower expression of downstream genes in an autoinduction system. In another word, the lux pR-3/5 has a lower efficiency than the lux pR(BBa_R0062). Similar to promoter lux pR, the expression is up-regulated by the action of LuxR protein combined with the signal molecule HSL.
mutable notes: We have generated two point mutants by site-directed mutagenesis from the sequence of lux pR(BBa_R0062). In these mutants, the G residue of position 5 was changed to a C and the C residue of position 3 was changed to a T respectively.
mutable provenance: The promoter lux pR (BBa_R0062).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: start
sequence annotation: -35
sequence annotation: K658008
sequence annotation: LuxR/HSL
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_K658008_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2148172
display id: annotation2148172
version: 1
top level: BBa_K658008
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K658008/annotation2148172/range2148172/1

https://synbiohub.org/public/igem/BBa_K658008/annotation2148172/range2148172/1
type: Range
persistent identity: range2148172
display id: range2148172
version: 1
direction: direction/forward
top level: BBa_K658008
start: 53
end: 53
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2148173
display id: annotation2148173
version: 1
top level: BBa_K658008
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K658008/annotation2148173/range2148173/1

https://synbiohub.org/public/igem/BBa_K658008/annotation2148173/range2148173/1
type: Range
persistent identity: range2148173
display id: range2148173
version: 1
direction: direction/forward
top level: BBa_K658008
start: 20
end: 25
orientation: inline

K658008
type: SequenceAnnotation
persistent identity: annotation2148175
display id: annotation2148175
version: 1
top level: BBa_K658008
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K658008/annotation2148175/range2148175/1

https://synbiohub.org/public/igem/BBa_K658008/annotation2148175/range2148175/1
type: Range
persistent identity: range2148175
display id: range2148175
version: 1
direction: direction/forward
top level: BBa_K658008
start: 1
end: 55
orientation: inline

LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2148176
display id: annotation2148176
version: 1
top level: BBa_K658008
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K658008/annotation2148176/range2148176/1

https://synbiohub.org/public/igem/BBa_K658008/annotation2148176/range2148176/1
type: Range
persistent identity: range2148176
display id: range2148176
version: 1
direction: direction/forward
top level: BBa_K658008
start: 1
end: 20
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2148174
display id: annotation2148174
version: 1
top level: BBa_K658008
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K658008/annotation2148174/range2148174/1

https://synbiohub.org/public/igem/BBa_K658008/annotation2148174/range2148174/1
type: Range
persistent identity: range2148174
display id: range2148174
version: 1
direction: direction/forward
top level: BBa_K658008
start: 42
end: 47
orientation: inline

BBa_K2110002
type: ComponentDefinition
persistent identity: BBa K2110002
display id: BBa_K2110002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TPA positive feedback regulatory gene.
creator: Le He
created: 2016-10-04T11:00:00Z
modified: 2016-10-05T11:22:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2578_
m user id: 32957
owner id: 32957
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This gene encodes the terephthalic acid(TPA) transporting protein. TPA is not a kind of nutrient substance for most organisms, including common chassis organisms like E.coli and Saccharomyces cerevisiae. However, as a kind of TPA degradable organism, RhodococcusjostiiRHA1 can transport TPA into their cells because of the TPA transporting protein genetpaK in their genome. This gene is also verified to be useful in Saccharomyces cerevisiae.
mutable notes: /
mutable provenance: It comes from the genome of RhodococcusjostiiRHA1.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BioBrick
sequence: https://synbiohub.org/public/igem/BBa_K2110002_sequence/1

BioBrick
type: SequenceAnnotation
persistent identity: annotation2486177
display id: annotation2486177
version: 1
top level: BBa_K2110002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K2110002/annotation2486177/range2486177/1

https://synbiohub.org/public/igem/BBa_K2110002/annotation2486177/range2486177/1
type: Range
persistent identity: range2486177
display id: range2486177
version: 1
direction: direction/forward
top level: BBa_K2110002
start: 1
end: 807
orientation: inline

BBa_K165034
type: ComponentDefinition
persistent identity: BBa K165034
display id: BBa_K165034
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Zif268-HIV bs + LexA bs + mCYC promoter
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:56Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The Zif268-HIV and LexA transcription factor binding sites on the mCYC minimal promoter (in that order.) Transcription regulation comes from Zif268-HIV and LexA-binding transcription factors.
mutable notes: -
mutable provenance: Constructed in our lab with parts from Caroline Ajo-Franklin and David Drubin.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K165011
component: BBa_K165031
sequence annotation: BBa_K165011
sequence annotation: BBa_K165031
sequence: https://synbiohub.org/public/igem/BBa_K165034_sequence/1

BBa_K165011
type: Component
persistent identity: component1995464
display id: component1995464
version: 1
top level: BBa_K165034
access: public
definition: https://synbiohub.org/public/igem/BBa_K165011/1

BBa_K165031
type: Component
persistent identity: component1995465
display id: component1995465
version: 1
top level: BBa_K165034
access: public
definition: BBa_K165031

BBa_K165011
type: SequenceAnnotation
persistent identity: annotation1995464
display id: annotation1995464
version: 1
top level: BBa_K165034
location: https://synbiohub.org/public/igem/BBa_K165034/annotation1995464/range1995464/1
component: BBa_K165011

https://synbiohub.org/public/igem/BBa_K165034/annotation1995464/range1995464/1
type: Range
persistent identity: range1995464
display id: range1995464
version: 1
direction: direction/reverse
top level: BBa_K165034
start: 1
end: 46
orientation: inline

BBa_K165031
type: SequenceAnnotation
persistent identity: annotation1995465
display id: annotation1995465
version: 1
top level: BBa_K165034
location: https://synbiohub.org/public/igem/BBa_K165034/annotation1995465/range1995465/1
component: BBa_K165031

https://synbiohub.org/public/igem/BBa_K165034/annotation1995465/range1995465/1
type: Range
persistent identity: range1995465
display id: range1995465
version: 1
direction: direction/reverse
top level: BBa_K165034
start: 55
end: 457
orientation: inline

Ara
type: ComponentDefinition
persistent identity: BBa K808000
display id: BBa_K808000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: araC-Pbad - Arabinose inducible regulatory promoter/repressor unit
creator: Valentina Herbring, Sebastian Palluk, Andreas Schmidt
created: 2012-08-10T11:00:00Z
modified: 2015-05-08T01:13:25Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1065_
m user id: 0
owner id: 11792
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This promoter can be induced by arabinose. In presens of arabinose the promotor is active.
mutable notes: non
mutable provenance: Guzman LM, Belin D, Carson MJ, Beckwith J. Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter. J Bacteriol. 1995 Jul;177(14):4121-30 Khlebnikov A, Skaug T, Keasling JD, modulation of gene expression from the arabinose-inducible araBAD promoter,J Ind Microbiol Biotechnol. 2002 Jul;29(1):34-7.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: AraO1
sequence annotation: AraO
sequence annotation: AraC
sequence annotation: AraPromoter
sequence: https://synbiohub.org/public/igem/BBa_K808000_sequence/1

AraO1
type: SequenceAnnotation
persistent identity: annotation2200759
display id: annotation2200759
version: 1
top level: Ara
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K808000/annotation2200759/range2200759/1

https://synbiohub.org/public/igem/BBa_K808000/annotation2200759/range2200759/1
type: Range
persistent identity: range2200759
display id: range2200759
version: 1
direction: direction/forward
top level: Ara
start: 1066
end: 1077
orientation: inline

AraO
type: SequenceAnnotation
persistent identity: annotation2200758
display id: annotation2200758
version: 1
top level: Ara
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K808000/annotation2200758/range2200758/1

https://synbiohub.org/public/igem/BBa_K808000/annotation2200758/range2200758/1
type: Range
persistent identity: range2200758
display id: range2200758
version: 1
direction: direction/forward
top level: Ara
start: 909
end: 924
orientation: inline

AraC
type: SequenceAnnotation
persistent identity: annotation2200757
display id: annotation2200757
version: 1
top level: Ara
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K808000/annotation2200757/range2200757/1

https://synbiohub.org/public/igem/BBa_K808000/annotation2200757/range2200757/1
type: Range
persistent identity: range2200757
display id: range2200757
version: 1
direction: direction/reverse
top level: Ara
start: 1
end: 879
orientation: inline

AraPromoter
type: SequenceAnnotation
persistent identity: annotation2200760
display id: annotation2200760
version: 1
top level: Ara
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K808000/annotation2200760/range2200760/1

https://synbiohub.org/public/igem/BBa_K808000/annotation2200760/range2200760/1
type: Range
persistent identity: range2200760
display id: range2200760
version: 1
direction: direction/forward
top level: Ara
start: 1153
end: 1181
orientation: inline

BBa_K389412
type: ComponentDefinition
persistent identity: BBa K389412
display id: BBa_K389412
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: vir promoter + sensitivity tuner + luciferase
creator: Jonas Aretz
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part combines a mutated virG response regulator from the VirA/G receptor system under the control of a constitutive promoter with a sensitivity tuner before a luciferase gene under the control of a vir promoter. The sensitivity tuner enhances a signal from a promoter so it enhances the readout luciferase.
mutable notes: sensitivity tuner to enhance the luciferase signal
mutable provenance: * luciferase from pGL4.10luc2 (Promega) * sensitivity tuner part, terminators and constitutive promoter from the partsregistry * ''virG'' was synthesized by Mr. Gene * ''vir'' promoter from ''Agrobacterium tumefaciens'' C58, TI-plasmid
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K389402
component: BBa_J23110
component: BBa_B0017
component: BBa_K389003
component: BBa_K389002
sequence annotation: BBa_K389002
sequence annotation: BBa_K389402
sequence annotation: BBa_B0017
sequence annotation: BBa_J23110
sequence annotation: BBa_K389003
sequence: https://synbiohub.org/public/igem/BBa_K389412_sequence/1

BBa_K389402
type: Component
persistent identity: component2300799
display id: component2300799
version: 1
top level: BBa_K389412
access: public
definition: https://synbiohub.org/public/igem/BBa_K389402/1

BBa_J23110
type: Component
persistent identity: component2300761
display id: component2300761
version: 1
top level: BBa_K389412
access: public
definition: https://synbiohub.org/public/igem/BBa_J23110/1

BBa_B0017
type: Component
persistent identity: component2300770
display id: component2300770
version: 1
top level: BBa_K389412
access: public
definition: https://synbiohub.org/public/igem/BBa_B0017/1

BBa_K389003
type: Component
persistent identity: component2300775
display id: component2300775
version: 1
top level: BBa_K389412
access: public
definition: Pvir

BBa_K389002
type: Component
persistent identity: component2300765
display id: component2300765
version: 1
top level: BBa_K389412
access: public
definition: https://synbiohub.org/public/igem/BBa_K389002/1

BBa_K389002
type: SequenceAnnotation
persistent identity: annotation2300765
display id: annotation2300765
version: 1
top level: BBa_K389412
location: https://synbiohub.org/public/igem/BBa_K389412/annotation2300765/range2300765/1
component: BBa_K389002

https://synbiohub.org/public/igem/BBa_K389412/annotation2300765/range2300765/1
type: Range
persistent identity: range2300765
display id: range2300765
version: 1
direction: direction/reverse
top level: BBa_K389412
start: 44
end: 783
orientation: inline

BBa_K389402
type: SequenceAnnotation
persistent identity: annotation2300799
display id: annotation2300799
version: 1
top level: BBa_K389412
location: https://synbiohub.org/public/igem/BBa_K389412/annotation2300799/range2300799/1
component: BBa_K389402

https://synbiohub.org/public/igem/BBa_K389412/annotation2300799/range2300799/1
type: Range
persistent identity: range2300799
display id: range2300799
version: 1
direction: direction/reverse
top level: BBa_K389412
start: 1134
end: 4020
orientation: inline

BBa_B0017
type: SequenceAnnotation
persistent identity: annotation2300770
display id: annotation2300770
version: 1
top level: BBa_K389412
location: https://synbiohub.org/public/igem/BBa_K389412/annotation2300770/range2300770/1
component: BBa_B0017

https://synbiohub.org/public/igem/BBa_K389412/annotation2300770/range2300770/1
type: Range
persistent identity: range2300770
display id: range2300770
version: 1
direction: direction/reverse
top level: BBa_K389412
start: 792
end: 959
orientation: inline

BBa_J23110
type: SequenceAnnotation
persistent identity: annotation2300761
display id: annotation2300761
version: 1
top level: BBa_K389412
location: https://synbiohub.org/public/igem/BBa_K389412/annotation2300761/range2300761/1
component: BBa_J23110

https://synbiohub.org/public/igem/BBa_K389412/annotation2300761/range2300761/1
type: Range
persistent identity: range2300761
display id: range2300761
version: 1
direction: direction/reverse
top level: BBa_K389412
start: 1
end: 35
orientation: inline

BBa_K389003
type: SequenceAnnotation
persistent identity: annotation2300775
display id: annotation2300775
version: 1
top level: BBa_K389412
location: https://synbiohub.org/public/igem/BBa_K389412/annotation2300775/range2300775/1
component: BBa_K389003

https://synbiohub.org/public/igem/BBa_K389412/annotation2300775/range2300775/1
type: Range
persistent identity: range2300775
display id: range2300775
version: 1
direction: direction/reverse
top level: BBa_K389412
start: 968
end: 1125
orientation: inline

BBa_K180000
type: ComponentDefinition
persistent identity: BBa K180000
display id: BBa_K180000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Hybrid promoter (trp & lac regulated -- tac pR)
creator: Richard Wilson, Stephanie Chambers
created: 2009-10-11T11:00:00Z
modified: 2015-05-08T01:11:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _278_
m user id: 0
owner id: 4082
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: The Ptac promoter is a functional hybrid promoter, derived from the trp and lac promoters, that are regulated by trp and lac [1]. The Ptac sequence has been extracted by PCR from the commercially available plasmid pMAL-pIII from New England Biolabs (N8101S), with the addition of the biobrick prefix and suffix sequences. [1] Proc. Natl. Acad. Sci. USA, Vol. 80, pp. 21-25
mutable notes: Sequence includes lacIq and Shine-Dalgarno sequence from pMAL-pIII prior to the Ptac promoter to ensure correct functionality of the promoter.
mutable provenance: pMAL-pIII (New England Biolabs N8101S)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: lacIq
sequence annotation: Ptac
sequence: https://synbiohub.org/public/igem/BBa_K180000_sequence/1

lacIq
type: SequenceAnnotation
persistent identity: annotation2044120
display id: annotation2044120
version: 1
top level: BBa_K180000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K180000/annotation2044120/range2044120/1

https://synbiohub.org/public/igem/BBa_K180000/annotation2044120/range2044120/1
type: Range
persistent identity: range2044120
display id: range2044120
version: 1
direction: direction/forward
top level: BBa_K180000
start: 1
end: 1082
orientation: inline

Ptac
type: SequenceAnnotation
persistent identity: annotation2044121
display id: annotation2044121
version: 1
top level: BBa_K180000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K180000/annotation2044121/range2044121/1

https://synbiohub.org/public/igem/BBa_K180000/annotation2044121/range2044121/1
type: Range
persistent identity: range2044121
display id: range2044121
version: 1
direction: direction/forward
top level: BBa_K180000
start: 1325
end: 1352
orientation: inline

Oct-4 prom
type: ComponentDefinition
persistent identity: BBa I10498
display id: BBa_I10498
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Oct-4 promoter
creator: Amanda Morris
created: 2006-05-13T11:00:00Z
modified: 2015-08-31T04:07:30Z
discontinued: false
dominant: false
experience: experience/None
group u list: _89_
m user id: 0
owner id: 622
owning group id: 89
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The part codes for the human Oct-4 promoter, which regulates expression of the Oct-4 gene. The Oct-4 gene is essential for pluripotency in cells and is downregulated once differentiation occurs. Sequence information is from GenBank (NM_002701) and shows the cis-regulatory mRNA that controls transcriptional activation of the human Oct-4 promoter.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I10498_sequence/1

OmpR
type: ComponentDefinition
persistent identity: BBa R0084
display id: BBa_R0084
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (OmpR, positive)
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Positively regulated, OmpR-controlled promoter. This promoter is taken from the upstream region of ompF. Phosphorylated OmpR binds to the three operator sites and activates transcription.
mutable notes: The promoter includes three OmpR binding sites: F1, F2, and F3. The promoter starts at -107 and goes up to the transcriptional start site at +1. A distant fourth binding site, F4, has been deleted. It is involved in negative regulation of ompF (Head, Tardy and Kenney, 1998), so its deletion is intended to make the promoter solely activating. The efficacy of this promoter versus the ompC upstream region (BBa_R0082) or the truncated ompC upstream region (BBa_R0083) is currently unknown.
mutable provenance: NC_000913 E. coli K12
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: F1 OmpR
sequence annotation: -10
sequence annotation: F2 OmpR
sequence annotation: -35
sequence annotation: F3 OmpR
sequence: https://synbiohub.org/public/igem/BBa_R0084_sequence/1

F1 OmpR
type: SequenceAnnotation
persistent identity: annotation301308
display id: annotation301308
version: 1
top level: OmpR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0084/annotation301308/range301308/1

https://synbiohub.org/public/igem/BBa_R0084/annotation301308/range301308/1
type: Range
persistent identity: range301308
display id: range301308
version: 1
direction: direction/forward
top level: OmpR
start: 11
end: 28
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation301318
display id: annotation301318
version: 1
top level: OmpR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0084/annotation301318/range301318/1

https://synbiohub.org/public/igem/BBa_R0084/annotation301318/range301318/1
type: Range
persistent identity: range301318
display id: range301318
version: 1
direction: direction/forward
top level: OmpR
start: 88
end: 93
orientation: inline

F2 OmpR
type: SequenceAnnotation
persistent identity: annotation301309
display id: annotation301309
version: 1
top level: OmpR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0084/annotation301309/range301309/1

https://synbiohub.org/public/igem/BBa_R0084/annotation301309/range301309/1
type: Range
persistent identity: range301309
display id: range301309
version: 1
direction: direction/forward
top level: OmpR
start: 31
end: 48
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation301317
display id: annotation301317
version: 1
top level: OmpR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0084/annotation301317/range301317/1

https://synbiohub.org/public/igem/BBa_R0084/annotation301317/range301317/1
type: Range
persistent identity: range301317
display id: range301317
version: 1
direction: direction/forward
top level: OmpR
start: 73
end: 78
orientation: inline

F3 OmpR
type: SequenceAnnotation
persistent identity: annotation301313
display id: annotation301313
version: 1
top level: OmpR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0084/annotation301313/range301313/1

https://synbiohub.org/public/igem/BBa_R0084/annotation301313/range301313/1
type: Range
persistent identity: range301313
display id: range301313
version: 1
direction: direction/forward
top level: OmpR
start: 51
end: 68
orientation: inline

BBa_K338029
type: ComponentDefinition
persistent identity: BBa K338029
display id: BBa_K338029
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: +OmpR, +(CinR-HSL) Double Promoter
creator: Lucas Hartsough
created: 2010-10-26T11:00:00Z
modified: 2015-05-08T01:12:07Z
discontinued: false
dominant: false
group u list: _454_
m user id: 0
owner id: 6013
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: An intermediate part designed to be used with the AND gate designed by Caltech iGEM '10: [http://2010.igem.org/Team:Caltech/Project Project Details].
mutable notes: Designed to be placed upstream of positive feedback mechanisms and desired output genes.
mutable provenance: The Registry of Standard Biological Parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0077
component: BBa_R0082
sequence annotation: BBa_R0077
sequence annotation: BBa_R0082
sequence: https://synbiohub.org/public/igem/BBa_K338029_sequence/1

BBa_R0077
type: Component
persistent identity: component2104982
display id: component2104982
version: 1
top level: BBa_K338029
access: public
definition: https://synbiohub.org/public/igem/BBa_R0077/1

BBa_R0082
type: Component
persistent identity: component2104977
display id: component2104977
version: 1
top level: BBa_K338029
access: public
definition: OmpR

BBa_R0077
type: SequenceAnnotation
persistent identity: annotation2104982
display id: annotation2104982
version: 1
top level: BBa_K338029
location: https://synbiohub.org/public/igem/BBa_K338029/annotation2104982/range2104982/1
component: BBa_R0077

https://synbiohub.org/public/igem/BBa_K338029/annotation2104982/range2104982/1
type: Range
persistent identity: range2104982
display id: range2104982
version: 1
direction: direction/reverse
top level: BBa_K338029
start: 117
end: 347
orientation: inline

BBa_R0082
type: SequenceAnnotation
persistent identity: annotation2104977
display id: annotation2104977
version: 1
top level: BBa_K338029
location: https://synbiohub.org/public/igem/BBa_K338029/annotation2104977/range2104977/1
component: BBa_R0082

https://synbiohub.org/public/igem/BBa_K338029/annotation2104977/range2104977/1
type: Range
persistent identity: range2104977
display id: range2104977
version: 1
direction: direction/reverse
top level: BBa_K338029
start: 1
end: 108
orientation: inline

BBa_K180011
type: ComponentDefinition
persistent identity: BBa K180011
display id: BBa_K180011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Rock - Primary plasmid (part 1) [RhlR generator]
creator: Richard Wilson, Stephanie Chambers
created: 2009-10-18T11:00:00Z
modified: 2015-05-08T01:11:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _278_
m user id: 0
owner id: 4082
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Part of the iGEM2009 Team:Southampton rock-paper-scissors 'rock' primary plasmid.
mutable notes: Constitutive promoter selected to allow for continuous expression of C0171.
mutable provenance: See relevant basic biobrick parts (J23119, B0034, C0171, B0015)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0012
component: BBa_B0034
component: BBa_J23119
component: BBa_C0171
component: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_J23119
sequence annotation: BBa_C0171
sequence: https://synbiohub.org/public/igem/BBa_K180011_sequence/1

BBa_B0012
type: Component
persistent identity: component2053908
display id: component2053908
version: 1
top level: BBa_K180011
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component2053904
display id: component2053904
version: 1
top level: BBa_K180011
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_J23119
type: Component
persistent identity: component2053902
display id: component2053902
version: 1
top level: BBa_K180011
access: public
definition: https://synbiohub.org/public/igem/BBa_J23119/1

BBa_C0171
type: Component
persistent identity: component2053905
display id: component2053905
version: 1
top level: BBa_K180011
access: public
definition: https://synbiohub.org/public/igem/BBa_C0171/1

BBa_B0010
type: Component
persistent identity: component2053906
display id: component2053906
version: 1
top level: BBa_K180011
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2053904
display id: annotation2053904
version: 1
top level: BBa_K180011
location: https://synbiohub.org/public/igem/BBa_K180011/annotation2053904/range2053904/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K180011/annotation2053904/range2053904/1
type: Range
persistent identity: range2053904
display id: range2053904
version: 1
direction: direction/reverse
top level: BBa_K180011
start: 44
end: 55
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2053908
display id: annotation2053908
version: 1
top level: BBa_K180011
location: https://synbiohub.org/public/igem/BBa_K180011/annotation2053908/range2053908/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K180011/annotation2053908/range2053908/1
type: Range
persistent identity: range2053908
display id: range2053908
version: 1
direction: direction/forward
top level: BBa_K180011
start: 887
end: 927
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2053906
display id: annotation2053906
version: 1
top level: BBa_K180011
location: https://synbiohub.org/public/igem/BBa_K180011/annotation2053906/range2053906/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K180011/annotation2053906/range2053906/1
type: Range
persistent identity: range2053906
display id: range2053906
version: 1
direction: direction/forward
top level: BBa_K180011
start: 799
end: 878
orientation: inline

BBa_J23119
type: SequenceAnnotation
persistent identity: annotation2053902
display id: annotation2053902
version: 1
top level: BBa_K180011
location: https://synbiohub.org/public/igem/BBa_K180011/annotation2053902/range2053902/1
component: BBa_J23119

https://synbiohub.org/public/igem/BBa_K180011/annotation2053902/range2053902/1
type: Range
persistent identity: range2053902
display id: range2053902
version: 1
direction: direction/reverse
top level: BBa_K180011
start: 1
end: 35
orientation: inline

BBa_C0171
type: SequenceAnnotation
persistent identity: annotation2053905
display id: annotation2053905
version: 1
top level: BBa_K180011
location: https://synbiohub.org/public/igem/BBa_K180011/annotation2053905/range2053905/1
component: BBa_C0171

https://synbiohub.org/public/igem/BBa_K180011/annotation2053905/range2053905/1
type: Range
persistent identity: range2053905
display id: range2053905
version: 1
direction: direction/reverse
top level: BBa_K180011
start: 62
end: 790
orientation: inline

BBa_K1486049
type: ComponentDefinition
persistent identity: BBa K1486049
display id: BBa_K1486049
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CpxR promoter FW + RFP
creator: EPFL iGem team 2014
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: to edit
mutable notes: to edit
mutable provenance: to edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_I13507
component: BBa_K1486046
sequence annotation: BBa_K1486046
sequence annotation: BBa_I13507
sequence: https://synbiohub.org/public/igem/BBa_K1486049_sequence/1

BBa_I13507
type: Component
persistent identity: component2398436
display id: component2398436
version: 1
top level: BBa_K1486049
access: public
definition: https://synbiohub.org/public/igem/BBa_I13507/1

BBa_K1486046
type: Component
persistent identity: component2398424
display id: component2398424
version: 1
top level: BBa_K1486049
access: public
definition: BBa_K1486046

BBa_K1486046
type: SequenceAnnotation
persistent identity: annotation2398424
display id: annotation2398424
version: 1
top level: BBa_K1486049
location: https://synbiohub.org/public/igem/BBa_K1486049/annotation2398424/range2398424/1
component: BBa_K1486046

https://synbiohub.org/public/igem/BBa_K1486049/annotation2398424/range2398424/1
type: Range
persistent identity: range2398424
display id: range2398424
version: 1
direction: direction/reverse
top level: BBa_K1486049
start: 1
end: 146
orientation: inline

BBa_I13507
type: SequenceAnnotation
persistent identity: annotation2398436
display id: annotation2398436
version: 1
top level: BBa_K1486049
location: https://synbiohub.org/public/igem/BBa_K1486049/annotation2398436/range2398436/1
component: BBa_I13507

https://synbiohub.org/public/igem/BBa_K1486049/annotation2398436/range2398436/1
type: Range
persistent identity: range2398436
display id: range2398436
version: 1
direction: direction/reverse
top level: BBa_K1486049
start: 155
end: 1015
orientation: inline

BBa_K1486048
type: ComponentDefinition
persistent identity: BBa K1486048
display id: BBa_K1486048
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CpxR reporter
creator: EPFL iGem team 2014
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Calgary's BBa_K339007 repaired (part didn't have the sequence they said they had.
mutable notes: to edit
mutable provenance: to edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_I13507
component: BBa_K135000
sequence annotation: BBa_I13507
sequence annotation: BBa_K135000
sequence: https://synbiohub.org/public/igem/BBa_K1486048_sequence/1

BBa_I13507
type: Component
persistent identity: component2398423
display id: component2398423
version: 1
top level: BBa_K1486048
access: public
definition: https://synbiohub.org/public/igem/BBa_I13507/1

BBa_K135000
type: Component
persistent identity: component2398411
display id: component2398411
version: 1
top level: BBa_K1486048
access: public
definition: BBa_K135000

BBa_I13507
type: SequenceAnnotation
persistent identity: annotation2398423
display id: annotation2398423
version: 1
top level: BBa_K1486048
location: https://synbiohub.org/public/igem/BBa_K1486048/annotation2398423/range2398423/1
component: BBa_I13507

https://synbiohub.org/public/igem/BBa_K1486048/annotation2398423/range2398423/1
type: Range
persistent identity: range2398423
display id: range2398423
version: 1
direction: direction/reverse
top level: BBa_K1486048
start: 64
end: 924
orientation: inline

BBa_K135000
type: SequenceAnnotation
persistent identity: annotation2398411
display id: annotation2398411
version: 1
top level: BBa_K1486048
location: https://synbiohub.org/public/igem/BBa_K1486048/annotation2398411/range2398411/1
component: BBa_K135000

https://synbiohub.org/public/igem/BBa_K1486048/annotation2398411/range2398411/1
type: Range
persistent identity: range2398411
display id: range2398411
version: 1
direction: direction/reverse
top level: BBa_K1486048
start: 1
end: 55
orientation: inline

BBa_I751502
type: ComponentDefinition
persistent identity: BBa I751502
display id: BBa_I751502
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: plux-lac hybrid promoter
creator: Kenichiro Iwasaki
created: 2007-10-24T11:00:00Z
modified: 2015-08-31T04:08:06Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _151_
m user id: 0
owner id: 2188
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: sense TWO INPUTS, activation by AHL and repression by LacI.
mutable notes: Compared with existing BioBrick hybrid promoter R0065, this promoter is different in that its sequence is much shorter with lux box and -35 region overlapping, and that it is repressed by LacI, not cI. Also, the output is clearly detected as shown in Fig. 3.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: luxR binding site
sequence annotation: plac
sequence annotation: -35
sequence annotation: O1
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_I751502_sequence/1

luxR binding site
type: SequenceAnnotation
persistent identity: annotation1955564
display id: annotation1955564
version: 1
top level: BBa_I751502
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I751502/annotation1955564/range1955564/1

https://synbiohub.org/public/igem/BBa_I751502/annotation1955564/range1955564/1
type: Range
persistent identity: range1955564
display id: range1955564
version: 1
direction: direction/forward
top level: BBa_I751502
start: 1
end: 19
orientation: inline

plac
type: SequenceAnnotation
persistent identity: annotation1955559
display id: annotation1955559
version: 1
top level: BBa_I751502
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I751502/annotation1955559/range1955559/1

https://synbiohub.org/public/igem/BBa_I751502/annotation1955559/range1955559/1
type: Range
persistent identity: range1955559
display id: range1955559
version: 1
direction: direction/forward
top level: BBa_I751502
start: 20
end: 74
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1955560
display id: annotation1955560
version: 1
top level: BBa_I751502
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I751502/annotation1955560/range1955560/1

https://synbiohub.org/public/igem/BBa_I751502/annotation1955560/range1955560/1
type: Range
persistent identity: range1955560
display id: range1955560
version: 1
direction: direction/forward
top level: BBa_I751502
start: 20
end: 26
orientation: inline

O1
type: SequenceAnnotation
persistent identity: annotation1955562
display id: annotation1955562
version: 1
top level: BBa_I751502
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I751502/annotation1955562/range1955562/1

https://synbiohub.org/public/igem/BBa_I751502/annotation1955562/range1955562/1
type: Range
persistent identity: range1955562
display id: range1955562
version: 1
direction: direction/forward
top level: BBa_I751502
start: 27
end: 41
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1955561
display id: annotation1955561
version: 1
top level: BBa_I751502
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I751502/annotation1955561/range1955561/1

https://synbiohub.org/public/igem/BBa_I751502/annotation1955561/range1955561/1
type: Range
persistent identity: range1955561
display id: range1955561
version: 1
direction: direction/forward
top level: BBa_I751502
start: 42
end: 48
orientation: inline

O1
type: SequenceAnnotation
persistent identity: annotation1955563
display id: annotation1955563
version: 1
top level: BBa_I751502
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I751502/annotation1955563/range1955563/1

https://synbiohub.org/public/igem/BBa_I751502/annotation1955563/range1955563/1
type: Range
persistent identity: range1955563
display id: range1955563
version: 1
direction: direction/forward
top level: BBa_I751502
start: 49
end: 74
orientation: inline

lux pR-5
type: ComponentDefinition
persistent identity: BBa K658007
display id: BBa_K658007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: position 5 mutated promoter lux pR-5 (luxR & HSL regulated)
creator: Yidan Hu
created: 2011-09-30T11:00:00Z
modified: 2015-05-08T01:13:02Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _835_
m user id: 0
owner id: 10207
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The position 5 mutated promoter lux pR-5 is a product of site-directed mutagenesis of promoter lux pR (BBa_R0062). The promoter lux pR is based on the Vibrio fischeri quorum sensing gene promoters. The position 5 mutated promoter lux pR-5 gives a higher expression of downstream genes in an autoinduction system. In another word, the lux pR-5 has a higher efficiency than the lux pR. Similar to promoter lux pR, the expression is up-regulated by the action of LuxR protein combined with the signal molecule HSL.
mutable notes: By PCR method the G residue of position 5 was changed to a C based on the sequence of the promoter lux pR(BBa_R0062).
mutable provenance: The promoter lux pR (BBa_R0062).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: start
sequence annotation: LuxR/HSL
sequence annotation: -35
sequence annotation: K658007
sequence: https://synbiohub.org/public/igem/BBa_K658007_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation2148171
display id: annotation2148171
version: 1
top level: lux pR-5
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K658007/annotation2148171/range2148171/1

https://synbiohub.org/public/igem/BBa_K658007/annotation2148171/range2148171/1
type: Range
persistent identity: range2148171
display id: range2148171
version: 1
direction: direction/forward
top level: lux pR-5
start: 42
end: 47
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2148167
display id: annotation2148167
version: 1
top level: lux pR-5
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K658007/annotation2148167/range2148167/1

https://synbiohub.org/public/igem/BBa_K658007/annotation2148167/range2148167/1
type: Range
persistent identity: range2148167
display id: range2148167
version: 1
direction: direction/forward
top level: lux pR-5
start: 53
end: 53
orientation: inline

LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2148169
display id: annotation2148169
version: 1
top level: lux pR-5
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K658007/annotation2148169/range2148169/1

https://synbiohub.org/public/igem/BBa_K658007/annotation2148169/range2148169/1
type: Range
persistent identity: range2148169
display id: range2148169
version: 1
direction: direction/forward
top level: lux pR-5
start: 1
end: 20
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2148170
display id: annotation2148170
version: 1
top level: lux pR-5
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K658007/annotation2148170/range2148170/1

https://synbiohub.org/public/igem/BBa_K658007/annotation2148170/range2148170/1
type: Range
persistent identity: range2148170
display id: range2148170
version: 1
direction: direction/forward
top level: lux pR-5
start: 20
end: 25
orientation: inline

K658007
type: SequenceAnnotation
persistent identity: annotation2148168
display id: annotation2148168
version: 1
top level: lux pR-5
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K658007/annotation2148168/range2148168/1

https://synbiohub.org/public/igem/BBa_K658007/annotation2148168/range2148168/1
type: Range
persistent identity: range2148168
display id: range2148168
version: 1
direction: direction/forward
top level: lux pR-5
start: 1
end: 55
orientation: inline

BBa_K135000
type: ComponentDefinition
persistent identity: BBa K135000
display id: BBa_K135000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCpxR (CpxR responsive promoter)
creator: Oliver Purcell
created: 2008-10-19T11:00:00Z
modified: 2015-05-08T01:10:01Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _183_
m user id: 0
owner id: 2796
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is suggested to be an adhesion responsive promoter. It contains the binding site for CpxR-P (phosphorylated CpxR). CpxR is one member of the two-component Cpx response to envelope stress. Briefly, envelope stress causes CpxA, an inner-membrane histidine Kinase to Phosphorylate CpxR, allowing it to bind to CpxR-P responsive regions and regulate downstream target genes. See 'Surface sensing and adhesion of Escherichia coli controlled by the Cpx-signaling pathway', Karen Otto and Thomas J. Silhavy, PNAS, Vol. 99., No, 4., 2287-2292. 2002.
mutable notes: None
mutable provenance: This part comes from the genome of E.coli TR235, as used in the PNAS paper of Otto and Silhavy mentioned previously. TR235 is K-12 MC4100 containing a pCpxR-LacZ fusion inserted at the (Lambda)att site, plus some other modifications (see Otto and Silhavy). The part is a portion of the CpxR responsive region used in these LacZ fusions.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: CpxR-P Binding site
sequence annotation: BBa_K135000
sequence: https://synbiohub.org/public/igem/BBa_K135000_sequence/1

CpxR-P Binding site
type: SequenceAnnotation
persistent identity: annotation1982751
display id: annotation1982751
version: 1
top level: BBa_K135000
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K135000/annotation1982751/range1982751/1

https://synbiohub.org/public/igem/BBa_K135000/annotation1982751/range1982751/1
type: Range
persistent identity: range1982751
display id: range1982751
version: 1
direction: direction/forward
top level: BBa_K135000
start: 1
end: 15
orientation: inline

BBa_K135000
type: SequenceAnnotation
persistent identity: annotation1982752
display id: annotation1982752
version: 1
top level: BBa_K135000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K135000/annotation1982752/range1982752/1

https://synbiohub.org/public/igem/BBa_K135000/annotation1982752/range1982752/1
type: Range
persistent identity: range1982752
display id: range1982752
version: 1
direction: direction/forward
top level: BBa_K135000
start: 1
end: 55
orientation: inline

BBa_K266006
type: ComponentDefinition
persistent identity: BBa K266006
display id: BBa_K266006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PAI+LasR -> LasI+GFP & AI+LuxR --| LasI+GFP
creator: UNAM FCIENCIAS-CINVESTAV IRA
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _363_
m user id: 0
owner id: 1955
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: BBa_K091146 promoter (PAI+LasR inducible & AI+LuxR repressible) controls the policistronic expresion of LasI enzyme and GFP, double terminator
mutable notes: None.
mutable provenance: The biobricks BBa_K266005 and BBa_E0840 where used to build this biobrick.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_K266005
component: BBa_E0840
sequence annotation: BBa_E0840
sequence annotation: BBa_K266005
sequence: https://synbiohub.org/public/igem/BBa_K266006_sequence/1

BBa_K266005
type: Component
persistent identity: component2267607
display id: component2267607
version: 1
top level: BBa_K266006
access: public
definition: BBa_K266005

BBa_E0840
type: Component
persistent identity: component2267618
display id: component2267618
version: 1
top level: BBa_K266006
access: public
definition: https://synbiohub.org/public/igem/BBa_E0840/1

BBa_E0840
type: SequenceAnnotation
persistent identity: annotation2267618
display id: annotation2267618
version: 1
top level: BBa_K266006
location: https://synbiohub.org/public/igem/BBa_K266006/annotation2267618/range2267618/1
component: BBa_E0840

https://synbiohub.org/public/igem/BBa_K266006/annotation2267618/range2267618/1
type: Range
persistent identity: range2267618
display id: range2267618
version: 1
direction: direction/reverse
top level: BBa_K266006
start: 828
end: 1705
orientation: inline

BBa_K266005
type: SequenceAnnotation
persistent identity: annotation2267607
display id: annotation2267607
version: 1
top level: BBa_K266006
location: https://synbiohub.org/public/igem/BBa_K266006/annotation2267607/range2267607/1
component: BBa_K266005

https://synbiohub.org/public/igem/BBa_K266006/annotation2267607/range2267607/1
type: Range
persistent identity: range2267607
display id: range2267607
version: 1
direction: direction/reverse
top level: BBa_K266006
start: 1
end: 819
orientation: inline

BBa_I761011
type: ComponentDefinition
persistent identity: BBa I761011
display id: BBa_I761011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CinR, CinL and glucose controlled promotor
creator: 2007 NYMU_Taiwan iGEM Team
created: 2007-10-18T11:00:00Z
modified: 2015-08-31T04:08:08Z
discontinued: false
dominant: false
experience: experience/None
group u list: _140_
m user id: 0
owner id: 2031
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This promotor is controlled by cinr, cinl and extrcellular glucose concentration. High extracellular glucose concentration will inhibit this promotor through the action of phosphorylated OmpR. Phosphorylated OmpR will bind the OmpR binding site of the promotor and block the movement of RNA polymerase. In contrast, the combined action of CinR and CinL will turn on the promotor. But only when the extracellular glucose level is low so OmpR dephosphorylate and dissociate from its binding site will this promotor become active. In essence, this promotor function as an AND gate, it will turn on if and only if the extracellular glucose level is low and there are some functional CinR and CinL protein inside the cell.
mutable notes: The key to the function of the promotor is the inhibitory ability of OmpR binding site. To ensure maximum affinity with phosphorylated OmpR, we use all three OmpR binding site.s.
mutable provenance: *BBa_R0078 *BBa_I761001
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_R0078
component: BBa_I761001
sequence annotation: BBa_I761001
sequence annotation: BBa_R0078
sequence: https://synbiohub.org/public/igem/BBa_I761011_sequence/1

BBa_R0078
type: Component
persistent identity: component1950118
display id: component1950118
version: 1
top level: BBa_I761011
access: public
definition: cinR

BBa_I761001
type: Component
persistent identity: component1950122
display id: component1950122
version: 1
top level: BBa_I761011
access: public
definition: https://synbiohub.org/public/igem/BBa_I761001/1

BBa_I761001
type: SequenceAnnotation
persistent identity: annotation1950122
display id: annotation1950122
version: 1
top level: BBa_I761011
location: https://synbiohub.org/public/igem/BBa_I761011/annotation1950122/range1950122/1
component: BBa_I761001

https://synbiohub.org/public/igem/BBa_I761011/annotation1950122/range1950122/1
type: Range
persistent identity: range1950122
display id: range1950122
version: 1
direction: direction/reverse
top level: BBa_I761011
start: 234
end: 295
orientation: inline

BBa_R0078
type: SequenceAnnotation
persistent identity: annotation1950118
display id: annotation1950118
version: 1
top level: BBa_I761011
location: https://synbiohub.org/public/igem/BBa_I761011/annotation1950118/range1950118/1
component: BBa_R0078

https://synbiohub.org/public/igem/BBa_I761011/annotation1950118/range1950118/1
type: Range
persistent identity: range1950118
display id: range1950118
version: 1
direction: direction/reverse
top level: BBa_I761011
start: 1
end: 225
orientation: inline

BBa_K266007
type: ComponentDefinition
persistent identity: BBa K266007
display id: BBa_K266007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Complex QS -> LuxI & LasI circuit
creator: UNAM FCIENCIAS-CINVESTAV IRA
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _363_
m user id: 0
owner id: 1955
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Complex Quorum sensing circuit that receives the signal of PAI+LasR and AI+LuxR to control the production of LuxI and LasI enzymes.
mutable notes: None
mutable provenance: The biobricks BBa_K266006 and BBa_K266000 where used to build this biobrick
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_K266000
component: BBa_K266006
sequence annotation: BBa_K266000
sequence annotation: BBa_K266006
sequence: https://synbiohub.org/public/igem/BBa_K266007_sequence/1

BBa_K266000
type: Component
persistent identity: component2297452
display id: component2297452
version: 1
top level: BBa_K266007
access: public
definition: BBa_K266000

BBa_K266006
type: Component
persistent identity: component2297433
display id: component2297433
version: 1
top level: BBa_K266007
access: public
definition: BBa_K266006

BBa_K266000
type: SequenceAnnotation
persistent identity: annotation2297452
display id: annotation2297452
version: 1
top level: BBa_K266007
location: https://synbiohub.org/public/igem/BBa_K266007/annotation2297452/range2297452/1
component: BBa_K266000

https://synbiohub.org/public/igem/BBa_K266007/annotation2297452/range2297452/1
type: Range
persistent identity: range2297452
display id: range2297452
version: 1
direction: direction/reverse
top level: BBa_K266007
start: 1714
end: 2676
orientation: inline

BBa_K266006
type: SequenceAnnotation
persistent identity: annotation2297433
display id: annotation2297433
version: 1
top level: BBa_K266007
location: https://synbiohub.org/public/igem/BBa_K266007/annotation2297433/range2297433/1
component: BBa_K266006

https://synbiohub.org/public/igem/BBa_K266007/annotation2297433/range2297433/1
type: Range
persistent identity: range2297433
display id: range2297433
version: 1
direction: direction/reverse
top level: BBa_K266007
start: 1
end: 1705
orientation: inline

BBa_K1520516
type: ComponentDefinition
persistent identity: BBa K1520516
display id: BBa_K1520516
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MC31-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter-Prlux-rbs-OMPA-golB-rbs-luxI-Ter
creator: yue wu
created: 2014-09-22T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: MC31-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter-Prlux-rbs-OMPA-golB-rbs-luxI-Ter It is a test of our positive feedback circuit for microcystin.
mutable notes: None
mutable provenance: MC31, luxI, Pcons2, luxR, Prlux, OMPA-golB, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1520029
component: BBa_K1520504
component: BBa_K1520002
component: BBa_K1520003
component: BBa_K1520507
component: BBa_R0062
sequence annotation: BBa_R0062
sequence annotation: BBa_K1520029
sequence annotation: BBa_K1520507
sequence annotation: BBa_K1520003
sequence annotation: BBa_K1520002
sequence annotation: BBa_K1520504
sequence: https://synbiohub.org/public/igem/BBa_K1520516_sequence/1

BBa_K1520029
type: Component
persistent identity: component2385696
display id: component2385696
version: 1
top level: BBa_K1520516
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520029/1

BBa_K1520029
type: Component
persistent identity: component2385718
display id: component2385718
version: 1
top level: BBa_K1520516
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520029/1

BBa_K1520504
type: Component
persistent identity: component2385717
display id: component2385717
version: 1
top level: BBa_K1520516
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520504/1

BBa_K1520002
type: Component
persistent identity: component2385692
display id: component2385692
version: 1
top level: BBa_K1520516
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520002/1

BBa_K1520003
type: Component
persistent identity: component2385702
display id: component2385702
version: 1
top level: BBa_K1520516
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520003/1

BBa_K1520507
type: Component
persistent identity: component2385714
display id: component2385714
version: 1
top level: BBa_K1520516
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520507/1

BBa_K1520504
type: Component
persistent identity: component2385695
display id: component2385695
version: 1
top level: BBa_K1520516
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520504/1

BBa_R0062
type: Component
persistent identity: component2385704
display id: component2385704
version: 1
top level: BBa_K1520516
access: public
definition: lux pR

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2385704
display id: annotation2385704
version: 1
top level: BBa_K1520516
location: https://synbiohub.org/public/igem/BBa_K1520516/annotation2385704/range2385704/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K1520516/annotation2385704/range2385704/1
type: Range
persistent identity: range2385704
display id: range2385704
version: 1
direction: direction/forward
top level: BBa_K1520516
start: 1934
end: 1988
orientation: inline

BBa_K1520029
type: SequenceAnnotation
persistent identity: annotation2385718
display id: annotation2385718
version: 1
top level: BBa_K1520516
location: https://synbiohub.org/public/igem/BBa_K1520516/annotation2385718/range2385718/1
component: BBa_K1520029

https://synbiohub.org/public/igem/BBa_K1520516/annotation2385718/range2385718/1
type: Range
persistent identity: range2385718
display id: range2385718
version: 1
direction: direction/reverse
top level: BBa_K1520516
start: 3482
end: 3610
orientation: inline

BBa_K1520507
type: SequenceAnnotation
persistent identity: annotation2385714
display id: annotation2385714
version: 1
top level: BBa_K1520516
location: https://synbiohub.org/public/igem/BBa_K1520516/annotation2385714/range2385714/1
component: BBa_K1520507

https://synbiohub.org/public/igem/BBa_K1520516/annotation2385714/range2385714/1
type: Range
persistent identity: range2385714
display id: range2385714
version: 1
direction: direction/reverse
top level: BBa_K1520516
start: 1995
end: 2804
orientation: inline

BBa_K1520003
type: SequenceAnnotation
persistent identity: annotation2385702
display id: annotation2385702
version: 1
top level: BBa_K1520516
location: https://synbiohub.org/public/igem/BBa_K1520516/annotation2385702/range2385702/1
component: BBa_K1520003

https://synbiohub.org/public/igem/BBa_K1520516/annotation2385702/range2385702/1
type: Range
persistent identity: range2385702
display id: range2385702
version: 1
direction: direction/reverse
top level: BBa_K1520516
start: 947
end: 1925
orientation: inline

BBa_K1520029
type: SequenceAnnotation
persistent identity: annotation2385696
display id: annotation2385696
version: 1
top level: BBa_K1520516
location: https://synbiohub.org/public/igem/BBa_K1520516/annotation2385696/range2385696/1
component: BBa_K1520029

https://synbiohub.org/public/igem/BBa_K1520516/annotation2385696/range2385696/1
type: Range
persistent identity: range2385696
display id: range2385696
version: 1
direction: direction/reverse
top level: BBa_K1520516
start: 810
end: 938
orientation: inline

BBa_K1520002
type: SequenceAnnotation
persistent identity: annotation2385692
display id: annotation2385692
version: 1
top level: BBa_K1520516
location: https://synbiohub.org/public/igem/BBa_K1520516/annotation2385692/range2385692/1
component: BBa_K1520002

https://synbiohub.org/public/igem/BBa_K1520516/annotation2385692/range2385692/1
type: Range
persistent identity: range2385692
display id: range2385692
version: 1
direction: direction/reverse
top level: BBa_K1520516
start: 1
end: 132
orientation: inline

BBa_K1520504
type: SequenceAnnotation
persistent identity: annotation2385717
display id: annotation2385717
version: 1
top level: BBa_K1520516
location: https://synbiohub.org/public/igem/BBa_K1520516/annotation2385717/range2385717/1
component: BBa_K1520504

https://synbiohub.org/public/igem/BBa_K1520516/annotation2385717/range2385717/1
type: Range
persistent identity: range2385717
display id: range2385717
version: 1
direction: direction/reverse
top level: BBa_K1520516
start: 2813
end: 3473
orientation: inline

BBa_K1520504
type: SequenceAnnotation
persistent identity: annotation2385695
display id: annotation2385695
version: 1
top level: BBa_K1520516
location: https://synbiohub.org/public/igem/BBa_K1520516/annotation2385695/range2385695/1
component: BBa_K1520504

https://synbiohub.org/public/igem/BBa_K1520516/annotation2385695/range2385695/1
type: Range
persistent identity: range2385695
display id: range2385695
version: 1
direction: direction/reverse
top level: BBa_K1520516
start: 141
end: 801
orientation: inline

BBa_I746361
type: ComponentDefinition
persistent identity: BBa I746361
display id: BBa_I746361
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PO promoter from P2 phage
creator: Stefan Milde
created: 2007-09-10T11:00:00Z
modified: 2015-08-31T04:08:04Z
discontinued: false
dominant: false
group u list: _116_
m user id: 0
owner id: 2122
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the PO promoter taken from the P2 phage genome. It is an inducible promoter that is activated by a class of activators, including P2 ogr (I746350), PSP3 pag (I746351) and phiR73 delta (I746352). These different activators should cause different levels of activity of the PO promoter.
mutable notes: no special considerations
mutable provenance: The source of the DNA is the P2 phage genome.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: PO
sequence: https://synbiohub.org/public/igem/BBa_I746361_sequence/1

PO
type: SequenceAnnotation
persistent identity: annotation1943784
display id: annotation1943784
version: 1
top level: BBa_I746361
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I746361/annotation1943784/range1943784/1

https://synbiohub.org/public/igem/BBa_I746361/annotation1943784/range1943784/1
type: Range
persistent identity: range1943784
display id: range1943784
version: 1
direction: direction/forward
top level: BBa_I746361
start: 1
end: 92
orientation: inline

BBa_K180016
type: ComponentDefinition
persistent identity: BBa K180016
display id: BBa_K180016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Rock-paper-scissors - Paper secondary plasmid [lux pR, LacI generator]
creator: Richard Wilson, Stephanie Chambers
created: 2009-10-18T11:00:00Z
modified: 2015-05-08T01:11:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _278_
m user id: 0
owner id: 4082
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: iGEM2009 Team:Southampton rock-paper-scissors 'Rock' secondary plasmid.
mutable notes: Regulated expression of LacI (C0012) by Lux pR (R0062).
mutable provenance: See relevant basic biobrick parts (R0062, B0034, C0012, B0015).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_C0012
component: BBa_B0034
component: BBa_B0015
component: BBa_R0062
sequence annotation: BBa_B0015
sequence annotation: BBa_B0034
sequence annotation: BBa_C0012
sequence annotation: BBa_R0062
sequence: https://synbiohub.org/public/igem/BBa_K180016_sequence/1

BBa_C0012
type: Component
persistent identity: component2256442
display id: component2256442
version: 1
top level: BBa_K180016
access: public
definition: https://synbiohub.org/public/igem/BBa_C0012/1

BBa_B0034
type: Component
persistent identity: component2256440
display id: component2256440
version: 1
top level: BBa_K180016
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0015
type: Component
persistent identity: component2256451
display id: component2256451
version: 1
top level: BBa_K180016
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_R0062
type: Component
persistent identity: component2256434
display id: component2256434
version: 1
top level: BBa_K180016
access: public
definition: lux pR

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2256451
display id: annotation2256451
version: 1
top level: BBa_K180016
location: https://synbiohub.org/public/igem/BBa_K180016/annotation2256451/range2256451/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K180016/annotation2256451/range2256451/1
type: Range
persistent identity: range2256451
display id: range2256451
version: 1
direction: direction/reverse
top level: BBa_K180016
start: 1243
end: 1371
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2256440
display id: annotation2256440
version: 1
top level: BBa_K180016
location: https://synbiohub.org/public/igem/BBa_K180016/annotation2256440/range2256440/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K180016/annotation2256440/range2256440/1
type: Range
persistent identity: range2256440
display id: range2256440
version: 1
direction: direction/reverse
top level: BBa_K180016
start: 64
end: 75
orientation: inline

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation2256442
display id: annotation2256442
version: 1
top level: BBa_K180016
location: https://synbiohub.org/public/igem/BBa_K180016/annotation2256442/range2256442/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_K180016/annotation2256442/range2256442/1
type: Range
persistent identity: range2256442
display id: range2256442
version: 1
direction: direction/forward
top level: BBa_K180016
start: 82
end: 1209
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2256434
display id: annotation2256434
version: 1
top level: BBa_K180016
location: https://synbiohub.org/public/igem/BBa_K180016/annotation2256434/range2256434/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K180016/annotation2256434/range2256434/1
type: Range
persistent identity: range2256434
display id: range2256434
version: 1
direction: direction/forward
top level: BBa_K180016
start: 1
end: 55
orientation: inline

BBa_K427003
type: ComponentDefinition
persistent identity: BBa K427003
display id: BBa_K427003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pm promoter of Mu bacteriophage
creator: Jan Marte Contreras Ortiz
created: 2010-10-21T11:00:00Z
modified: 2015-05-08T01:12:29Z
discontinued: false
dominant: false
group u list: _544_
m user id: 0
owner id: 6602
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Pm is the middle promoter of the Mu bacteriophage. It is in charge of the transcription of the activator protein C. Pm is activated by a product of the early transcription, protein Mor. It can be used to build a sensitivity tuner alongside this protein, this construct can be used to increase the amount of POPS in a certain genetic construction.
mutable notes: No special considerations
mutable provenance: Synthetized from the reported sequence of Pm promoter from Mu bacteriophage.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Pm
sequence: https://synbiohub.org/public/igem/BBa_K427003_sequence/1

Pm
type: SequenceAnnotation
persistent identity: annotation2092367
display id: annotation2092367
version: 1
top level: BBa_K427003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K427003/annotation2092367/range2092367/1

https://synbiohub.org/public/igem/BBa_K427003/annotation2092367/range2092367/1
type: Range
persistent identity: range2092367
display id: range2092367
version: 1
direction: direction/forward
top level: BBa_K427003
start: 1
end: 71
orientation: inline

pBad/araC
type: ComponentDefinition
persistent identity: BBa I0500
display id: BBa_I0500
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Inducible pBad/araC promoter
creator: Sri Kosuri
created: 2003-12-05T12:00:00Z
modified: 2015-08-31T04:07:29Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: PC
sequence annotation: AraC
sequence annotation: Pbad
sequence: https://synbiohub.org/public/igem/BBa_I0500_sequence/1

PC
type: SequenceAnnotation
persistent identity: annotation1498842
display id: annotation1498842
version: 1
top level: pBad/araC
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I0500/annotation1498842/range1498842/1

https://synbiohub.org/public/igem/BBa_I0500/annotation1498842/range1498842/1
type: Range
persistent identity: range1498842
display id: range1498842
version: 1
direction: direction/forward
top level: pBad/araC
start: 1043
end: 1114
orientation: inline

AraC
type: SequenceAnnotation
persistent identity: annotation1498843
display id: annotation1498843
version: 1
top level: pBad/araC
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I0500/annotation1498843/range1498843/1

https://synbiohub.org/public/igem/BBa_I0500/annotation1498843/range1498843/1
type: Range
persistent identity: range1498843
display id: range1498843
version: 1
direction: direction/forward
top level: pBad/araC
start: 1
end: 1079
orientation: inline

Pbad
type: SequenceAnnotation
persistent identity: annotation1498841
display id: annotation1498841
version: 1
top level: pBad/araC
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I0500/annotation1498841/range1498841/1

https://synbiohub.org/public/igem/BBa_I0500/annotation1498841/range1498841/1
type: Range
persistent identity: range1498841
display id: range1498841
version: 1
direction: direction/forward
top level: pBad/araC
start: 1080
end: 1210
orientation: inline

BBa_K427004
type: ComponentDefinition
persistent identity: BBa K427004
display id: BBa_K427004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pmom promoter of Mu bacteriophage
creator: Jan Marte Contreras Ortiz
created: 2010-10-21T11:00:00Z
modified: 2015-05-08T01:12:29Z
discontinued: false
dominant: false
group u list: _544_
m user id: 0
owner id: 6602
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Pmom is one of the four late promoters of the Mu bacteriophage. It can be activated by the C protein, which is produced by the middle promoter Pm. Pmom works because of a special configuration of the DNA. It can be used to create a sensitivity tuner alongside the C protein of the same phage. This construction can be used to increase the POPS output of a promoter.
mutable notes: The design of this part had no special considerations
mutable provenance: Synthetized from the reported sequence of Pmom promoter from Mu bacteriophage
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Pmom
sequence: https://synbiohub.org/public/igem/BBa_K427004_sequence/1

Pmom
type: SequenceAnnotation
persistent identity: annotation2092368
display id: annotation2092368
version: 1
top level: BBa_K427004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K427004/annotation2092368/range2092368/1

https://synbiohub.org/public/igem/BBa_K427004/annotation2092368/range2092368/1
type: Range
persistent identity: range2092368
display id: range2092368
version: 1
direction: direction/forward
top level: BBa_K427004
start: 1
end: 79
orientation: inline

BBa_K2110005
type: ComponentDefinition
persistent identity: BBa K2110005
display id: BBa_K2110005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TPA inducible RFP expression system
creator: Le He
created: 2016-10-05T11:00:00Z
modified: 2016-10-15T06:00:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2578_
m user id: 32964
owner id: 32957
owning group id: 9
status: status/Planning
bookmark: false
mutable description: PETase gene encodes the PETase enzyme which can degrade PET to terephthalic acid (TPA) and ethylene glycol (EG) with another enzyme MHETase. However, the product TPA can cause inhibition of the reaction and slow down it. This expression system consists of the TPA inducible Leader sequence, the enhanced promoter PGK1(BBa_K2110012), the PETase gene(BBa_K2110800) and Terminator CYC1(BBa_K2110000). It can be induced by product TPA so that this is a positive feedback system. This system is used in Saccharomyces cerevisiae.
mutable notes: /
mutable provenance: BBa_K2110012 BBa_K2110800 BBa_K2110000
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_E1010
component: BBa_K2110000
component: BBa_K2110001
component: BBa_K2110012
sequence annotation: BBa_K2110012
sequence annotation: BBa_E1010
sequence annotation: BBa_K2110001
sequence annotation: BBa_K2110000
sequence: https://synbiohub.org/public/igem/BBa_K2110005_sequence/1

BBa_E1010
type: Component
persistent identity: component2512254
display id: component2512254
version: 1
top level: BBa_K2110005
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_K2110000
type: Component
persistent identity: component2512255
display id: component2512255
version: 1
top level: BBa_K2110005
access: public
definition: https://synbiohub.org/public/igem/BBa_K2110000/1

BBa_K2110001
type: Component
persistent identity: component2512249
display id: component2512249
version: 1
top level: BBa_K2110005
access: public
definition: https://synbiohub.org/public/igem/BBa_K2110001/1

BBa_K2110012
type: Component
persistent identity: component2512251
display id: component2512251
version: 1
top level: BBa_K2110005
access: public
definition: https://synbiohub.org/public/igem/BBa_K2110012/1

BBa_K2110012
type: SequenceAnnotation
persistent identity: annotation2512251
display id: annotation2512251
version: 1
top level: BBa_K2110005
location: https://synbiohub.org/public/igem/BBa_K2110005/annotation2512251/range2512251/1
component: BBa_K2110012

https://synbiohub.org/public/igem/BBa_K2110005/annotation2512251/range2512251/1
type: Range
persistent identity: range2512251
display id: range2512251
version: 1
direction: direction/reverse
top level: BBa_K2110005
start: 105
end: 885
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2512254
display id: annotation2512254
version: 1
top level: BBa_K2110005
location: https://synbiohub.org/public/igem/BBa_K2110005/annotation2512254/range2512254/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K2110005/annotation2512254/range2512254/1
type: Range
persistent identity: range2512254
display id: range2512254
version: 1
direction: direction/reverse
top level: BBa_K2110005
start: 892
end: 1597
orientation: inline

BBa_K2110001
type: SequenceAnnotation
persistent identity: annotation2512249
display id: annotation2512249
version: 1
top level: BBa_K2110005
location: https://synbiohub.org/public/igem/BBa_K2110005/annotation2512249/range2512249/1
component: BBa_K2110001

https://synbiohub.org/public/igem/BBa_K2110005/annotation2512249/range2512249/1
type: Range
persistent identity: range2512249
display id: range2512249
version: 1
direction: direction/reverse
top level: BBa_K2110005
start: 1
end: 96
orientation: inline

BBa_K2110000
type: SequenceAnnotation
persistent identity: annotation2512255
display id: annotation2512255
version: 1
top level: BBa_K2110005
location: https://synbiohub.org/public/igem/BBa_K2110005/annotation2512255/range2512255/1
component: BBa_K2110000

https://synbiohub.org/public/igem/BBa_K2110005/annotation2512255/range2512255/1
type: Range
persistent identity: range2512255
display id: range2512255
version: 1
direction: direction/reverse
top level: BBa_K2110005
start: 1606
end: 1853
orientation: inline

BBa_K180018
type: ComponentDefinition
persistent identity: BBa K180018
display id: BBa_K180018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Scissors - Primary plasmid (part 1) [LasR generator]
creator: Richard Wilson, Stephanie Chambers
created: 2009-10-18T11:00:00Z
modified: 2015-05-08T01:11:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _278_
m user id: 0
owner id: 4082
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Part of the iGEM2009 Team:Southampton rock-paper-scissors 'scissors' primary plasmid.
mutable notes: Constitutive promoter selected to allow for continuous expression of C0179.
mutable provenance: See relevant basic biobrick parts (J23119, B0034, C0179, B0015)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0012
component: BBa_B0010
component: BBa_J23119
component: BBa_B0034
component: BBa_C0179
sequence annotation: BBa_C0179
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_J23119
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K180018_sequence/1

BBa_B0012
type: Component
persistent identity: component2053918
display id: component2053918
version: 1
top level: BBa_K180018
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component2053916
display id: component2053916
version: 1
top level: BBa_K180018
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_J23119
type: Component
persistent identity: component2053912
display id: component2053912
version: 1
top level: BBa_K180018
access: public
definition: https://synbiohub.org/public/igem/BBa_J23119/1

BBa_B0034
type: Component
persistent identity: component2053914
display id: component2053914
version: 1
top level: BBa_K180018
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0179
type: Component
persistent identity: component2053915
display id: component2053915
version: 1
top level: BBa_K180018
access: public
definition: https://synbiohub.org/public/igem/BBa_C0179/1

BBa_C0179
type: SequenceAnnotation
persistent identity: annotation2053915
display id: annotation2053915
version: 1
top level: BBa_K180018
location: https://synbiohub.org/public/igem/BBa_K180018/annotation2053915/range2053915/1
component: BBa_C0179

https://synbiohub.org/public/igem/BBa_K180018/annotation2053915/range2053915/1
type: Range
persistent identity: range2053915
display id: range2053915
version: 1
direction: direction/reverse
top level: BBa_K180018
start: 62
end: 784
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2053916
display id: annotation2053916
version: 1
top level: BBa_K180018
location: https://synbiohub.org/public/igem/BBa_K180018/annotation2053916/range2053916/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K180018/annotation2053916/range2053916/1
type: Range
persistent identity: range2053916
display id: range2053916
version: 1
direction: direction/forward
top level: BBa_K180018
start: 793
end: 872
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2053918
display id: annotation2053918
version: 1
top level: BBa_K180018
location: https://synbiohub.org/public/igem/BBa_K180018/annotation2053918/range2053918/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K180018/annotation2053918/range2053918/1
type: Range
persistent identity: range2053918
display id: range2053918
version: 1
direction: direction/forward
top level: BBa_K180018
start: 881
end: 921
orientation: inline

BBa_J23119
type: SequenceAnnotation
persistent identity: annotation2053912
display id: annotation2053912
version: 1
top level: BBa_K180018
location: https://synbiohub.org/public/igem/BBa_K180018/annotation2053912/range2053912/1
component: BBa_J23119

https://synbiohub.org/public/igem/BBa_K180018/annotation2053912/range2053912/1
type: Range
persistent identity: range2053912
display id: range2053912
version: 1
direction: direction/reverse
top level: BBa_K180018
start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2053914
display id: annotation2053914
version: 1
top level: BBa_K180018
location: https://synbiohub.org/public/igem/BBa_K180018/annotation2053914/range2053914/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K180018/annotation2053914/range2053914/1
type: Range
persistent identity: range2053914
display id: range2053914
version: 1
direction: direction/reverse
top level: BBa_K180018
start: 44
end: 55
orientation: inline

BBa_K259013
type: ComponentDefinition
persistent identity: BBa K259013
display id: BBa_K259013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AraC-RBS-GFP-Terminator
creator: Petros Mina
created: 2009-10-14T11:00:00Z
modified: 2015-05-08T01:11:42Z
discontinued: false
dominant: false
group u list: _357_
m user id: 0
owner id: 5318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: A part used to quantify the AraC-RBS construct K259007 created by the BCCS-Bristol iGEM'09 team.
mutable notes: RFC10 assembly
mutable provenance: This part was designed with the specified biobricks from the parts registry. AraC occurs naturally in E.coli. GFP occurs in jellyfish species, RBS designed by the Anderson family and Terminator by Resma Shetty.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0012
component: BBa_J61100
component: BBa_E0040
component: BBa_R0081
component: BBa_R0080
component: BBa_B0011
sequence annotation: BBa_R0081
sequence annotation: BBa_B0012
sequence annotation: BBa_R0080
sequence annotation: BBa_B0011
sequence annotation: BBa_J61100
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_K259013_sequence/1

BBa_B0012
type: Component
persistent identity: component2041447
display id: component2041447
version: 1
top level: BBa_K259013
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_J61100
type: Component
persistent identity: component2041444
display id: component2041444
version: 1
top level: BBa_K259013
access: public
definition: https://synbiohub.org/public/igem/BBa_J61100/1

BBa_E0040
type: Component
persistent identity: component2041446
display id: component2041446
version: 1
top level: BBa_K259013
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_R0081
type: Component
persistent identity: component2041436
display id: component2041436
version: 1
top level: BBa_K259013
access: public
definition: https://synbiohub.org/public/igem/BBa_R0081/1

BBa_R0080
type: Component
persistent identity: component2041443
display id: component2041443
version: 1
top level: BBa_K259013
access: public
definition: AraC

BBa_B0011
type: Component
persistent identity: component2041451
display id: component2041451
version: 1
top level: BBa_K259013
access: public
definition: https://synbiohub.org/public/igem/BBa_B0011/1

BBa_R0081
type: SequenceAnnotation
persistent identity: annotation2041436
display id: annotation2041436
version: 1
top level: BBa_K259013
location: https://synbiohub.org/public/igem/BBa_K259013/annotation2041436/range2041436/1
component: BBa_R0081

https://synbiohub.org/public/igem/BBa_K259013/annotation2041436/range2041436/1
type: Range
persistent identity: range2041436
display id: range2041436
version: 1
direction: direction/reverse
top level: BBa_K259013
start: 1
end: 183
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2041447
display id: annotation2041447
version: 1
top level: BBa_K259013
location: https://synbiohub.org/public/igem/BBa_K259013/annotation2041447/range2041447/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K259013/annotation2041447/range2041447/1
type: Range
persistent identity: range2041447
display id: range2041447
version: 1
direction: direction/forward
top level: BBa_K259013
start: 1095
end: 1135
orientation: inline

BBa_R0080
type: SequenceAnnotation
persistent identity: annotation2041443
display id: annotation2041443
version: 1
top level: BBa_K259013
location: https://synbiohub.org/public/igem/BBa_K259013/annotation2041443/range2041443/1
component: BBa_R0080

https://synbiohub.org/public/igem/BBa_K259013/annotation2041443/range2041443/1
type: Range
persistent identity: range2041443
display id: range2041443
version: 1
direction: direction/reverse
top level: BBa_K259013
start: 192
end: 340
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation2041451
display id: annotation2041451
version: 1
top level: BBa_K259013
location: https://synbiohub.org/public/igem/BBa_K259013/annotation2041451/range2041451/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_K259013/annotation2041451/range2041451/1
type: Range
persistent identity: range2041451
display id: range2041451
version: 1
direction: direction/forward
top level: BBa_K259013
start: 1144
end: 1189
orientation: inline

BBa_J61100
type: SequenceAnnotation
persistent identity: annotation2041444
display id: annotation2041444
version: 1
top level: BBa_K259013
location: https://synbiohub.org/public/igem/BBa_K259013/annotation2041444/range2041444/1
component: BBa_J61100

https://synbiohub.org/public/igem/BBa_K259013/annotation2041444/range2041444/1
type: Range
persistent identity: range2041444
display id: range2041444
version: 1
direction: direction/reverse
top level: BBa_K259013
start: 349
end: 360
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2041446
display id: annotation2041446
version: 1
top level: BBa_K259013
location: https://synbiohub.org/public/igem/BBa_K259013/annotation2041446/range2041446/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K259013/annotation2041446/range2041446/1
type: Range
persistent identity: range2041446
display id: range2041446
version: 1
direction: direction/reverse
top level: BBa_K259013
start: 367
end: 1086
orientation: inline

BBa_J05217
type: ComponentDefinition
persistent identity: BBa J05217
display id: BBa_J05217
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Regulator for R2-YAP7
creator: Alexander Roth
created: 2005-09-07T11:00:00Z
modified: 2015-08-31T04:08:15Z
discontinued: false
dominant: false
group u list: _34_
m user id: 0
owner id: 412
owning group id: 34
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: binding sites for R3-ATF6 and R4-cMaf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J05217_sequence/1

BBa_K112400
type: ComponentDefinition
persistent identity: BBa K112400
display id: BBa_K112400
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter for grpE gene - Heat Shock and Ultrasound Sensitive
creator: Christina Brown
created: 2008-10-19T11:00:00Z
modified: 2015-05-08T01:09:17Z
discontinued: false
dominant: true
group u list: _224_
m user id: 0
owner id: 3241
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: According to Vollmer, et. al in their 1998 Applied and Enviormental Microbiology paper, "Bacterial Stress Responses to 1-Megahertz Pulsed Ultrasound in the Presence of Microbubble", the promoter for the grpE gene, a known heat shock promoter, is upregulated 24 times when cells are exposed to high frequency ultrasound and microbubble cavitation.
mutable notes: none
mutable provenance: cloned from E. coli MG1655 genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K112400_sequence/1

BBa_K2012000
type: ComponentDefinition
persistent identity: BBa K2012000
display id: BBa_K2012000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: c-di-GMP tandem riboswitche bc3-5
creator: Pan Chu
created: 2016-08-04T11:00:00Z
modified: 2016-10-09T01:16:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2479_
m user id: 25212
owner id: 25212
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Three complete c-di-GMP riboswitches (Bc3, Bc4 and Bc5 RNA) with similar structures, which are arranged in tandem to constitute a triple-tandem (Bc3-5 RNA) riboswitch in the 5′-UTR of the cspABCDE mRNA in Bacillus thuringiensis subsp. chinensis CT-43.
mutable notes: We designed the sequence of bc3-5 by add perfix and suffix which are belong to BioBrick RFC 10.
mutable provenance: From Bacillus thuringiensis subsp. chinensis CT-43 In the CT-43 genome19,20 (GenBank accession: NC_017208.1), five type I c-di-GMP riboswitches named Bc1, Bc2, Bc3, Bc4 and Bc5 RNA (Supplementary Tables S1???S5) were annotated in the Rfam database (http://rfam.sanger.ac.uk/search). Zhou, H., et al. (2016). "Characterization of a natural triple-tandem c-di-GMP riboswitch and application of the riboswitch-based dual-fluorescence reporter." Sci Rep 6: 20871.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Inverter
sequence: https://synbiohub.org/public/igem/BBa_K2012000_sequence/1

BBa_I746104
type: ComponentDefinition
persistent identity: BBa I746104
display id: BBa_I746104
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: P2 promoter in agr operon from S. aureus
creator: Zhizhen Zhao
created: 2007-08-30T11:00:00Z
modified: 2015-08-31T04:08:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _116_
m user id: 0
owner id: 2121
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The agr P2 operon is an autocatalytic sensory transduction system in Staphylococcus aureus. P2 promoter regulates the synthesis of AgrB (a transmembrane protein) and AgrD (precursor of AIP autoinducing peptide). AIP binds to AgrC, a membrane binding receptor and it phosphorylates AgrA. The phosphorylated AgrA increases the expression level from P2 promoter.
mutable notes: Codon usage has been optimised for E. coli (which also seems to give good results for B. subtilis
mutable provenance: The section of the sequence constituting the P2 promoter is subject to rather more debate than the sequences of the coding regions. [http://jb.asm.org/cgi/content/full/186/22/7549 Koenig, Robbin L., Ray, Jessica L., Maleki, Soheila J., Smeltzer, Mark S., Hurlburt, Barry K. Staphylococcus aureus AgrA Binding to the RNAIII-agr Regulatory Region J. Bacteriol. 2004 186: 7549-7555] shows the areas of the sequence to which phosphorylated AgrA binds while L Rao, R K Karls, and M J Betley: In vitro transcription of pathogenesis-related genes by purified RNA polymerase from Staphylococcus aureus. Bacteriol. 1995 May; 177(10): 2609???2614. shows the transcription start sites; we took the overall extent of the promoter region from that labelled in Morfeldt, E., K. Tegmark, and S. Arvidson. 1996. Transcriptional control of the agr-dependent virulence gene regulator, RNAIII, in Staphylococcus aureus. Mol. Microbiol. 21:1227???1237. (not available online).
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I746104_sequence/1

pT7
type: ComponentDefinition
persistent identity: BBa K525998
display id: BBa_K525998
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter T7 and RBS
creator: Anna Drong
created: 2011-09-12T11:00:00Z
modified: 2015-05-08T01:12:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _690_
m user id: 0
owner id: 8543
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Promoter T7 and RBS
mutable notes: Synthesis
mutable provenance: Synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: T7 promoter
sequence annotation: RBS
sequence annotation: B0034
sequence: https://synbiohub.org/public/igem/BBa_K525998_sequence/1

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2129432
display id: annotation2129432
version: 1
top level: pT7
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525998/annotation2129432/range2129432/1

https://synbiohub.org/public/igem/BBa_K525998/annotation2129432/range2129432/1
type: Range
persistent identity: range2129432
display id: range2129432
version: 1
direction: direction/forward
top level: pT7
start: 1
end: 20
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2129433
display id: annotation2129433
version: 1
top level: pT7
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525998/annotation2129433/range2129433/1

https://synbiohub.org/public/igem/BBa_K525998/annotation2129433/range2129433/1
type: Range
persistent identity: range2129433
display id: range2129433
version: 1
direction: direction/forward
top level: pT7
start: 24
end: 27
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2127788
display id: annotation2127788
version: 1
top level: pT7
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525998/annotation2127788/range2127788/1

https://synbiohub.org/public/igem/BBa_K525998/annotation2127788/range2127788/1
type: Range
persistent identity: range2127788
display id: range2127788
version: 1
direction: direction/forward
top level: pT7
start: 21
end: 32
orientation: inline

BBa_K753000
type: ComponentDefinition
persistent identity: BBa K753000
display id: BBa_K753000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Yeast FIG1 promoter
creator: Rachel Engeler
created: 2012-08-28T11:00:00Z
modified: 2015-05-08T01:13:12Z
discontinued: false
dominant: false
group u list: _1004_
m user id: 0
owner id: 11572
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Promoter of the FIG1 gene in yeast. The transcription factor Ste12, activated in the yeast pheromone pathway, binds to the FIG1 promoter and initiate the expression of the FIG1 gene. Five pheromone response elements (PRE, consensus sequence ATGAAACA) have been identifi?ed in the 5'-upstream regulatory sequence of the FIG1 gene. Phosphorylated Ste12 binds to them and thereby activates transcription. The PREs in the FIG1 promoter di?ffer slightly from the PRE consensus sequence ATGAAACA: Sequence Location TGAAACG -405 TGAAACC* -217 TGACACA* -206 TGAAACG -173 TGAAATA -133 Sites marked with an asterisk occur on the opposite strand The strictly regulated transcriptional feature of the FIG1 promoter can be useful when investigating the pheromone pathway. The promoter can be used to express reporter genes in response to activation of the pheromone pathway. Reference: Erdman S, Lin L, Malczynski M, Snyder M. Pheromone-regulated Genes Required for Yeast Mating Diff?erentiation. The Journal of cell biology. 1998;140(3):461-483.
mutable notes: The length of the promoter was chosen to 450 bp as recommended by Kondo group of Kobe University of Japan. Togawa S, Ishii J, Ishikura A, Tanaka T, Ogino C, Kondo A. Importance of asparagine residues at positions 13 and 26 on the amino-terminal domain of human somatostatin receptor subtype-5 in signalling. J Biochem. 2010 Jun;147(6):867-73
mutable provenance: The sequence has been amplified directly from genomic yeast DNA.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K753000_sequence/1

BBa_K392001
type: ComponentDefinition
persistent identity: BBa K392001
display id: BBa_K392001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: yeast ENO2 promoter
creator: Tadashi Nakamura, Shuhei Yasumoto, Takahiro Saka, Saya Kakuda
created: 2010-10-09T11:00:00Z
modified: 2015-09-25T08:35:36Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _528_
m user id: 15693
owner id: 6220
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: LacI promoter with RBS attached downstream.
mutable notes: Assembled by 3A method.
mutable provenance: Registry parts BBa_R0010 (promoter) and BBa_B0034 (RBS).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K392001_sequence/1

P3
type: ComponentDefinition
persistent identity: BBa K100002
display id: BBa_K100002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Edited Xylose Regulated Bi-Directional Operator 2
creator: Garrett Tobin
created: 2008-08-17T11:00:00Z
modified: 2015-05-08T01:08:41Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _213_
m user id: 0
owner id: 1752
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part contains the operator region controlling transcription for the xyl operon. This regulatory region is dependent upon the binding of CRP-cAMP and XylR bound to xylose for activation. Upon binding, transcription is activated in both directions (xylFGH and xylAB facing). This part has a scrambled CRP binding site and and altered RNA ploymerase binding site that was designed to more resemble the consensus sequence at an intermediate level.
mutable notes: Both directions were kept in the sequence of the part even though it is intended to work in the right facing direction because of possible looping that may occur as a transcriptional control. The RNA polymerase binding site was altered to make binding stronger, attempting to allow activation when XylR-xylose alone is bound (the absence of CRP-cAMP).
mutable provenance: Taken from E. Coli genome bases 3728830-3729092, base pairs changed at RNA polymerase bining sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: RNA poly. Binding Site
sequence annotation: Scrambled CRP Binding Site
sequence: https://synbiohub.org/public/igem/BBa_K100002_sequence/1

RNA poly. Binding Site
type: SequenceAnnotation
persistent identity: annotation1972265
display id: annotation1972265
version: 1
top level: P3
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K100002/annotation1972265/range1972265/1

https://synbiohub.org/public/igem/BBa_K100002/annotation1972265/range1972265/1
type: Range
persistent identity: range1972265
display id: range1972265
version: 1
direction: direction/forward
top level: P3
start: 265
end: 270
orientation: inline

RNA poly. Binding Site
type: SequenceAnnotation
persistent identity: annotation1972259
display id: annotation1972259
version: 1
top level: P3
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K100002/annotation1972259/range1972259/1

https://synbiohub.org/public/igem/BBa_K100002/annotation1972259/range1972259/1
type: Range
persistent identity: range1972259
display id: range1972259
version: 1
direction: direction/forward
top level: P3
start: 45
end: 50
orientation: inline

RNA poly. Binding Site
type: SequenceAnnotation
persistent identity: annotation1972264
display id: annotation1972264
version: 1
top level: P3
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K100002/annotation1972264/range1972264/1

https://synbiohub.org/public/igem/BBa_K100002/annotation1972264/range1972264/1
type: Range
persistent identity: range1972264
display id: range1972264
version: 1
direction: direction/forward
top level: P3
start: 243
end: 248
orientation: inline

Scrambled CRP Binding Site
type: SequenceAnnotation
persistent identity: annotation1972262
display id: annotation1972262
version: 1
top level: P3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K100002/annotation1972262/range1972262/1

https://synbiohub.org/public/igem/BBa_K100002/annotation1972262/range1972262/1
type: Range
persistent identity: range1972262
display id: range1972262
version: 1
direction: direction/forward
top level: P3
start: 88
end: 104
orientation: inline

RNA poly. Binding Site
type: SequenceAnnotation
persistent identity: annotation1972260
display id: annotation1972260
version: 1
top level: P3
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K100002/annotation1972260/range1972260/1

https://synbiohub.org/public/igem/BBa_K100002/annotation1972260/range1972260/1
type: Range
persistent identity: range1972260
display id: range1972260
version: 1
direction: direction/reverse
top level: P3
start: 21
end: 26
orientation: inline

BBa_J102001
type: ComponentDefinition
persistent identity: BBa J102001
display id: BBa_J102001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Reverse Lux Promoter
creator: Michael Pettigrew
created: 2010-07-01T11:00:00Z
modified: 2015-08-31T04:08:24Z
discontinued: false
dominant: false
group u list: _580_
m user id: 0
owner id: 5944
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This promoter is the right promoter in the lux cassette of Vibrio Fischeri. There is weak constitutive expression of downstream genes. Expression of downstream genes can be up-regulated by the action of the LuxR activator protein in complex with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein can form a complex with two molecules of the AHL. This causes a conformational change in the protein, allowing it to bind to a site on the lux promoter and induce transcription.
mutable notes: None.
mutable provenance: This part was obtained by taking the reverse complement of R0062, ordering appropriate oligos, then annealing and extending them.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: promoter
sequence: https://synbiohub.org/public/igem/BBa_J102001_sequence/1

promoter
type: SequenceAnnotation
persistent identity: annotation2072374
display id: annotation2072374
version: 1
top level: BBa_J102001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J102001/annotation2072374/range2072374/1

https://synbiohub.org/public/igem/BBa_J102001/annotation2072374/range2072374/1
type: Range
persistent identity: range2072374
display id: range2072374
version: 1
direction: direction/reverse
top level: BBa_J102001
start: 1
end: 55
orientation: inline

BBa_K1315000
type: ComponentDefinition
persistent identity: BBa K1315000
display id: BBa_K1315000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pqsR
creator: Dimitrios Michailidis
created: 2014-09-19T11:00:00Z
modified: 2015-05-08T01:09:48Z
discontinued: true
dominant: false
experience: experience/None
group u list: _1690_
m user id: 0
owner id: 21812
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: The Pseydomonas quinolone signal (PQS) as well as its precursor 2-heptyl-4-quinolone (HHQ)are very important in mechanisms controlling virulence in Pseudomonas aeruginosa. P. aeruginosa has a key role in Cystic Fibrosis (CF). PQS can initiate signalling for various processes like virulence, motility, and biofilm formation. A good number of these processes are facilitated by PqsR, which is a transcriptional factor. An activated PqsR will go to the nucleus of the cells and activate the respective genes. http://www.ncbi.nlm.nih.gov/pubmed/21539583 http://www.ncbi.nlm.nih.gov/pubmed/?term=pqsr-dependent+and+pqsr-independent+regulation+of+motility+and+biofilm+formation+by+pqs http://www.ncbi.nlm.nih.gov/pubmed/12393198
mutable notes: The sequence did not have any restriction sites to remove so it was immediately PCRed out of the genome and had the registry prefix and suffix added to it. It was then ligated into the pSB1C3 vector.
mutable provenance: The source of this part is the genome of Pseudomonas aeruginosa PAO1 provided by the Robert Ryan lab in the Molecular Microbiology department of the University of Dundee, UK.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: PqsR
sequence: https://synbiohub.org/public/igem/BBa_K1315000_sequence/1

PqsR
type: SequenceAnnotation
persistent identity: annotation2384759
display id: annotation2384759
version: 1
top level: BBa_K1315000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1315000/annotation2384759/range2384759/1

https://synbiohub.org/public/igem/BBa_K1315000/annotation2384759/range2384759/1
type: Range
persistent identity: range2384759
display id: range2384759
version: 1
direction: direction/forward
top level: BBa_K1315000
start: 1
end: 999
orientation: inline

Lock3
type: ComponentDefinition
persistent identity: BBa J01080
display id: BBa_J01080
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lock3
creator: Golden Bear
created: 2005-11-29T12:00:00Z
modified: 2015-08-31T04:08:12Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _13_
m user id: 0
owner id: 395
owning group id: 13
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence: https://synbiohub.org/public/igem/BBa_J01080_sequence/1

BBa_J64800
type: ComponentDefinition
persistent identity: BBa J64800
display id: BBa_J64800
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RHLR/RHLI Inducible & LasR/LasI repressible Promoter
creator: laura lavery
created: 2007-03-24T12:00:00Z
modified: 2015-05-08T01:08:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 1431
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is similar to BBa_R0079 but has an added binding site for RHLR/RHLI in between the -35 and -10 region. Thus this promoter is induced by LasR/LasI but repressed by RHLR/RHLi binding.
mutable notes: There is a 17bp spacer between the -35 and -10 regions and the binding site for RHLR/RHLI is 20bp long. I was able to fit the whole binding site in the spacer region because the last three base pairs of the -35 region and the first three base pairs of the RHLR/RHLI binding site are the same.
mutable provenance: Rust L. et. al., "Analysis of the Pseudomonas aeruginosa Elastase (lasB) Regulatory Region". Journal of Bacteriology, 1996.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: LasR/LasI
sequence annotation: -35
sequence annotation: RHLR/RHLI
sequence: https://synbiohub.org/public/igem/BBa_J64800_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation1921063
display id: annotation1921063
version: 1
top level: BBa_J64800
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J64800/annotation1921063/range1921063/1

https://synbiohub.org/public/igem/BBa_J64800/annotation1921063/range1921063/1
type: Range
persistent identity: range1921063
display id: range1921063
version: 1
direction: direction/forward
top level: BBa_J64800
start: 42
end: 47
orientation: inline

LasR/LasI
type: SequenceAnnotation
persistent identity: annotation1921061
display id: annotation1921061
version: 1
top level: BBa_J64800
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_J64800/annotation1921061/range1921061/1

https://synbiohub.org/public/igem/BBa_J64800/annotation1921061/range1921061/1
type: Range
persistent identity: range1921061
display id: range1921061
version: 1
direction: direction/forward
top level: BBa_J64800
start: 23
end: 40
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1921062
display id: annotation1921062
version: 1
top level: BBa_J64800
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J64800/annotation1921062/range1921062/1

https://synbiohub.org/public/igem/BBa_J64800/annotation1921062/range1921062/1
type: Range
persistent identity: range1921062
display id: range1921062
version: 1
direction: direction/forward
top level: BBa_J64800
start: 19
end: 24
orientation: inline

RHLR/RHLI
type: SequenceAnnotation
persistent identity: annotation1921060
display id: annotation1921060
version: 1
top level: BBa_J64800
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J64800/annotation1921060/range1921060/1

https://synbiohub.org/public/igem/BBa_J64800/annotation1921060/range1921060/1
type: Range
persistent identity: range1921060
display id: range1921060
version: 1
direction: direction/forward
top level: BBa_J64800
start: 1
end: 23
orientation: inline

BBa_K112118
type: ComponentDefinition
persistent identity: BBa K112118
display id: BBa_K112118
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rrnB P1 promoter
creator: Bing Xia
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:14Z
discontinued: false
dominant: false
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable provenance: PCR mv028/mv029 on rrnB native sequence (721bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112118 ----------------------------------------------- mv028 Forward Biobricking of rrnB P1 promoter cgataGAATTCatgAGATCTatgcactaaaacgtatcctacgcccg mv029 Reverse Biobricking of rrnB P1 promoter cgttaGGATCCcgccttcccgctacagagtcaagc
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K112118_sequence/1

BBa_K136000
type: ComponentDefinition
persistent identity: BBa K136000
display id: BBa_K136000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter of fliL gene from E. coli flagella
creator: Cyprien Maisonnier
created: 2008-08-11T11:00:00Z
modified: 2015-05-08T01:10:02Z
discontinued: true
dominant: false
experience: experience/None
group u list: _211_
m user id: 0
owner id: 3012
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: ''fliL'' is a class 2 gene that is part of the construction of the flagella ( ''Ordering Genes in a Flagella Pathway by Analysis of Expression Kinetics from Living Bacteria'' - S. Kalir, J. McClure, K. Pabbaraju, C. Southward, M. Ronen, S. Leibler, M. G. Surette, U. Alon, ). Its promoter is consitutively repressed. It is activated by flhD4C2 and by fliA with different forces ( ''Using a Quantitative Blueprint to Reprogram the Dynamics of the Flagella Gene Network'' - Shiraz Kalir and Uri Alon ). A deeper characterization is in project
mutable notes: The primers used to amplify this sequence are : Forward : TCGAATTCGCGGCCGCTTCTAGAGCAAGGGCGTGTAACAGGCAAC Reverse : TCCTGCAGCGGCCGCTACTAGTAGTCATGTGTTGCGGTCTTCCTGTG
mutable provenance: This part comes from the genome of ''E.coli'' K12 strain MG1655.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Initiation start for transcription 1
sequence annotation: Initiation start for transcription 2
sequence annotation: FlhDC binding site 2
sequence annotation: FlhDC binding site 1
sequence: https://synbiohub.org/public/igem/BBa_K136000_sequence/1

Initiation start for transcription 1
type: SequenceAnnotation
persistent identity: annotation1971566
display id: annotation1971566
version: 1
top level: BBa_K136000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K136000/annotation1971566/range1971566/1

https://synbiohub.org/public/igem/BBa_K136000/annotation1971566/range1971566/1
type: Range
persistent identity: range1971566
display id: range1971566
version: 1
direction: direction/forward
top level: BBa_K136000
start: 116
end: 116
orientation: inline

Initiation start for transcription 2
type: SequenceAnnotation
persistent identity: annotation1971567
display id: annotation1971567
version: 1
top level: BBa_K136000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K136000/annotation1971567/range1971567/1

https://synbiohub.org/public/igem/BBa_K136000/annotation1971567/range1971567/1
type: Range
persistent identity: range1971567
display id: range1971567
version: 1
direction: direction/forward
top level: BBa_K136000
start: 127
end: 127
orientation: inline

FlhDC binding site 2
type: SequenceAnnotation
persistent identity: annotation1971565
display id: annotation1971565
version: 1
top level: BBa_K136000
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K136000/annotation1971565/range1971565/1

https://synbiohub.org/public/igem/BBa_K136000/annotation1971565/range1971565/1
type: Range
persistent identity: range1971565
display id: range1971565
version: 1
direction: direction/forward
top level: BBa_K136000
start: 70
end: 85
orientation: inline

FlhDC binding site 1
type: SequenceAnnotation
persistent identity: annotation1971564
display id: annotation1971564
version: 1
top level: BBa_K136000
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K136000/annotation1971564/range1971564/1

https://synbiohub.org/public/igem/BBa_K136000/annotation1971564/range1971564/1
type: Range
persistent identity: range1971564
display id: range1971564
version: 1
direction: direction/forward
top level: BBa_K136000
start: 44
end: 59
orientation: inline

Cpn10/60
type: ComponentDefinition
persistent identity: BBa K538310
display id: BBa_K538310
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cpn10/60 operon (pBAD)
creator: Bas Stringer
created: 2011-09-07T11:00:00Z
modified: 2015-05-08T01:12:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _704_
m user id: 0
owner id: 9985
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K538000
component: BBa_K206000
component: BBa_B0034
component: BBa_K538001
sequence annotation: BBa_K538001
sequence annotation: BBa_K538000
sequence annotation: BBa_B0034
sequence annotation: BBa_K206000
sequence: https://synbiohub.org/public/igem/BBa_K538310_sequence/1

BBa_K538000
type: Component
persistent identity: component2222800
display id: component2222800
version: 1
top level: Cpn10/60
access: public
definition: https://synbiohub.org/public/igem/BBa_K538000/1

BBa_K206000
type: Component
persistent identity: component2222795
display id: component2222795
version: 1
top level: Cpn10/60
access: public
definition: pBAD

BBa_B0034
type: Component
persistent identity: component2222797
display id: component2222797
version: 1
top level: Cpn10/60
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K538001
type: Component
persistent identity: component2222806
display id: component2222806
version: 1
top level: Cpn10/60
access: public
definition: https://synbiohub.org/public/igem/BBa_K538001/1

BBa_B0034
type: Component
persistent identity: component2222802
display id: component2222802
version: 1
top level: Cpn10/60
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K538001
type: SequenceAnnotation
persistent identity: annotation2222806
display id: annotation2222806
version: 1
top level: Cpn10/60
location: https://synbiohub.org/public/igem/BBa_K538310/annotation2222806/range2222806/1
component: BBa_K538001

https://synbiohub.org/public/igem/BBa_K538310/annotation2222806/range2222806/1
type: Range
persistent identity: range2222806
display id: range2222806
version: 1
direction: direction/reverse
top level: Cpn10/60
start: 480
end: 2129
orientation: inline

BBa_K538000
type: SequenceAnnotation
persistent identity: annotation2222800
display id: annotation2222800
version: 1
top level: Cpn10/60
location: https://synbiohub.org/public/igem/BBa_K538310/annotation2222800/range2222800/1
component: BBa_K538000

https://synbiohub.org/public/igem/BBa_K538310/annotation2222800/range2222800/1
type: Range
persistent identity: range2222800
display id: range2222800
version: 1
direction: direction/reverse
top level: Cpn10/60
start: 157
end: 453
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2222797
display id: annotation2222797
version: 1
top level: Cpn10/60
location: https://synbiohub.org/public/igem/BBa_K538310/annotation2222797/range2222797/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K538310/annotation2222797/range2222797/1
type: Range
persistent identity: range2222797
display id: range2222797
version: 1
direction: direction/reverse
top level: Cpn10/60
start: 139
end: 150
orientation: inline

BBa_K206000
type: SequenceAnnotation
persistent identity: annotation2222795
display id: annotation2222795
version: 1
top level: Cpn10/60
location: https://synbiohub.org/public/igem/BBa_K538310/annotation2222795/range2222795/1
component: BBa_K206000

https://synbiohub.org/public/igem/BBa_K538310/annotation2222795/range2222795/1
type: Range
persistent identity: range2222795
display id: range2222795
version: 1
direction: direction/reverse
top level: Cpn10/60
start: 1
end: 130
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2222802
display id: annotation2222802
version: 1
top level: Cpn10/60
location: https://synbiohub.org/public/igem/BBa_K538310/annotation2222802/range2222802/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K538310/annotation2222802/range2222802/1
type: Range
persistent identity: range2222802
display id: range2222802
version: 1
direction: direction/reverse
top level: Cpn10/60
start: 462
end: 473
orientation: inline

BBa_I13406
type: ComponentDefinition
persistent identity: BBa I13406
display id: BBa_I13406
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pbad/AraC with extra REN sites
creator: jkm
created: 2005-05-03T11:00:00Z
modified: 2015-08-31T04:07:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 253
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: I0500 with extra trailing restriction sites: Eco-Xba-I0500-HindIII-NcoI-Spe-Pst
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I13406_sequence/1

OmpR
type: ComponentDefinition
persistent identity: BBa R0083
display id: BBa_R0083
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (OmpR, positive)
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Positively regulated, OmpR-controlled promoter. This promoter is derived from the upstream region of ompC. Phosphorylated OmpR binds to the operator site and activates transcription. This is a truncated version of BBa_R0082.
mutable notes: The promoter deletes the C2 and C3 regions of BBa_R0082 (bases 37-74 in BBa_R0082) and inserts an 8-base linker before the -35 and -10 signal region. The efficacy of this promoter versus the full ompC upstream region (BBa_R0082) or the ompF upstream region (BBa_R0084) is currently unknown. The a at base 41 is mutated to t to avoid XbaI site. Speculation that this was a previous assembly site.
mutable provenance: NC_000193 E. coli K12
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: A
sequence annotation: -10
sequence annotation: C1 OmpR
sequence annotation: -35
sequence annotation: linker
sequence: https://synbiohub.org/public/igem/BBa_R0083_sequence/1

A
type: SequenceAnnotation
persistent identity: annotation306881
display id: annotation306881
version: 1
top level: OmpR
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_R0083/annotation306881/range306881/1

https://synbiohub.org/public/igem/BBa_R0083/annotation306881/range306881/1
type: Range
persistent identity: range306881
display id: range306881
version: 1
direction: direction/forward
top level: OmpR
start: 41
end: 41
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation301226
display id: annotation301226
version: 1
top level: OmpR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0083/annotation301226/range301226/1

https://synbiohub.org/public/igem/BBa_R0083/annotation301226/range301226/1
type: Range
persistent identity: range301226
display id: range301226
version: 1
direction: direction/forward
top level: OmpR
start: 68
end: 73
orientation: inline

C1 OmpR
type: SequenceAnnotation
persistent identity: annotation301221
display id: annotation301221
version: 1
top level: OmpR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0083/annotation301221/range301221/1

https://synbiohub.org/public/igem/BBa_R0083/annotation301221/range301221/1
type: Range
persistent identity: range301221
display id: range301221
version: 1
direction: direction/forward
top level: OmpR
start: 13
end: 30
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation301224
display id: annotation301224
version: 1
top level: OmpR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0083/annotation301224/range301224/1

https://synbiohub.org/public/igem/BBa_R0083/annotation301224/range301224/1
type: Range
persistent identity: range301224
display id: range301224
version: 1
direction: direction/forward
top level: OmpR
start: 45
end: 50
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation301223
display id: annotation301223
version: 1
top level: OmpR
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_R0083/annotation301223/range301223/1

https://synbiohub.org/public/igem/BBa_R0083/annotation301223/range301223/1
type: Range
persistent identity: range301223
display id: range301223
version: 1
direction: direction/forward
top level: OmpR
start: 37
end: 44
orientation: inline

BBa_K125100
type: ComponentDefinition
persistent identity: BBa K125100
display id: BBa_K125100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: nir promoter from Synechocystis sp. PCC6803
creator: Krystle Salazar and Grace Kwan
created: 2008-07-23T11:00:00Z
modified: 2015-05-08T01:09:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _
m user id: 0
owner id: 3229
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The ''nir'' promoter is nitrate inducible.
mutable provenance: The ''nirA'' promoter originates from ''Synechocystis'' sp. PCC6803.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Ntc-B binding motif
sequence annotation: Ntc-A binding motif
sequence annotation: nir promoter
sequence annotation: -10 sequence
sequence: https://synbiohub.org/public/igem/BBa_K125100_sequence/1

Ntc-B binding motif
type: SequenceAnnotation
persistent identity: annotation1968488
display id: annotation1968488
version: 1
top level: BBa_K125100
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K125100/annotation1968488/range1968488/1

https://synbiohub.org/public/igem/BBa_K125100/annotation1968488/range1968488/1
type: Range
persistent identity: range1968488
display id: range1968488
version: 1
direction: direction/forward
top level: BBa_K125100
start: 5
end: 20
orientation: inline

Ntc-A binding motif
type: SequenceAnnotation
persistent identity: annotation1968489
display id: annotation1968489
version: 1
top level: BBa_K125100
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K125100/annotation1968489/range1968489/1

https://synbiohub.org/public/igem/BBa_K125100/annotation1968489/range1968489/1
type: Range
persistent identity: range1968489
display id: range1968489
version: 1
direction: direction/forward
top level: BBa_K125100
start: 36
end: 44
orientation: inline

nir promoter
type: SequenceAnnotation
persistent identity: annotation1968486
display id: annotation1968486
version: 1
top level: BBa_K125100
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K125100/annotation1968486/range1968486/1

https://synbiohub.org/public/igem/BBa_K125100/annotation1968486/range1968486/1
type: Range
persistent identity: range1968486
display id: range1968486
version: 1
direction: direction/forward
top level: BBa_K125100
start: 1
end: 88
orientation: inline

-10 sequence
type: SequenceAnnotation
persistent identity: annotation1968487
display id: annotation1968487
version: 1
top level: BBa_K125100
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K125100/annotation1968487/range1968487/1

https://synbiohub.org/public/igem/BBa_K125100/annotation1968487/range1968487/1
type: Range
persistent identity: range1968487
display id: range1968487
version: 1
direction: direction/forward
top level: BBa_K125100
start: 73
end: 78
orientation: inline

p_qrr4
type: ComponentDefinition
persistent identity: BBa K131017
display id: BBa_K131017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: p_qrr4 from Vibrio harveyi
creator: Sonja Georgijevic
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:09:47Z
discontinued: false
dominant: true
experience: experience/None
group u list: _259_
m user id: 0
owner id: 1653
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: qrr4 promoter from Vibrio harveyi
mutable notes: 5' upstream sequence of qrr4 containing -12 and -24 regions (specific for Sigma factor 54)
mutable provenance: >gi|156527546:c1833759-1833376 Vibrio harveyi ATCC BAA-1116 chromosome II, complete sequence
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -12 region
sequence annotation: -24 region
sequence: https://synbiohub.org/public/igem/BBa_K131017_sequence/1

-12 region
type: SequenceAnnotation
persistent identity: annotation1990440
display id: annotation1990440
version: 1
top level: p_qrr4
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K131017/annotation1990440/range1990440/1

https://synbiohub.org/public/igem/BBa_K131017/annotation1990440/range1990440/1
type: Range
persistent identity: range1990440
display id: range1990440
version: 1
direction: direction/forward
top level: p_qrr4
start: 262
end: 264
orientation: inline

-24 region
type: SequenceAnnotation
persistent identity: annotation1990436
display id: annotation1990436
version: 1
top level: p_qrr4
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K131017/annotation1990436/range1990436/1

https://synbiohub.org/public/igem/BBa_K131017/annotation1990436/range1990436/1
type: Range
persistent identity: range1990436
display id: range1990436
version: 1
direction: direction/forward
top level: p_qrr4
start: 250
end: 254
orientation: inline

BBa_K1315006
type: ComponentDefinition
persistent identity: BBa K1315006
display id: BBa_K1315006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: The manA promoter from Xanthomonas campestris
creator: Dimitrios Michailidis
created: 2014-09-25T11:00:00Z
modified: 2015-05-08T01:09:50Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1690_
m user id: 0
owner id: 21812
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the promoter manA from Xcc.
mutable notes: The sequence did not have any restriction sites to remove so it was immediately PCRed out of the genome and had the registry prefix and suffix added to it. It was then ligated into the pSB1C3 vector.
mutable provenance: The source of this part is Xanthomonas campestris pv. campestris 8004 genomic DNA which was provided by the Robert Ryan lab in the Molecular Microbiology department of the University of Dundee, UK.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: manA
sequence: https://synbiohub.org/public/igem/BBa_K1315006_sequence/1

manA
type: SequenceAnnotation
persistent identity: annotation2386904
display id: annotation2386904
version: 1
top level: BBa_K1315006
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1315006/annotation2386904/range2386904/1

https://synbiohub.org/public/igem/BBa_K1315006/annotation2386904/range2386904/1
type: Range
persistent identity: range2386904
display id: range2386904
version: 1
direction: direction/forward
top level: BBa_K1315006
start: 1
end: 250
orientation: inline

cI+luxR
type: ComponentDefinition
persistent identity: BBa R0065
display id: BBa_R0065
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (lambda cI and luxR regulated -- hybrid)
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: cI repressor negatively regulates this promoter and LuxR activates its transcription.The effect of cI is dominant over LuxR. This part is based on the LuxR and cI repressor regulated hybrid promoter designed and tested by Ron Weiss. It requires the binding of two cI repressor dimers for maximal repression and contains two cI repressor binding sites namely, OR1 and OR2. This promoter is leaky in the sense that 'some' transcription is seen in the absence of both cI and LuxR. LuxI cI activity of promoter + + zero + - maximum - + zero - - leaky (no quantitative information)
mutable notes: This part was designed based on the LuxR and cI repressor regulated hybrid promoter tested by Ron Weiss and the LuxR-LuxICDABE sequence annotated by Tom Knight AF170104 .
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: Lux Box
sequence annotation: OR1 cI
sequence annotation: -35
sequence annotation: lux p(R) start
sequence annotation: OR2 cI
sequence annotation: BBa_R0065
sequence: https://synbiohub.org/public/igem/BBa_R0065_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation1986781
display id: annotation1986781
version: 1
top level: cI+luxR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986781/range1986781/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986781/range1986781/1
type: Range
persistent identity: range1986781
display id: range1986781
version: 1
direction: direction/forward
top level: cI+luxR
start: 94
end: 97
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986776
display id: annotation1986776
version: 1
top level: cI+luxR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986776/range1986776/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986776/range1986776/1
type: Range
persistent identity: range1986776
display id: range1986776
version: 1
direction: direction/forward
top level: cI+luxR
start: 47
end: 52
orientation: inline

Lux Box
type: SequenceAnnotation
persistent identity: annotation1986775
display id: annotation1986775
version: 1
top level: cI+luxR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986775/range1986775/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986775/range1986775/1
type: Range
persistent identity: range1986775
display id: range1986775
version: 1
direction: direction/forward
top level: cI+luxR
start: 6
end: 25
orientation: inline

OR1 cI
type: SequenceAnnotation
persistent identity: annotation1986780
display id: annotation1986780
version: 1
top level: cI+luxR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986780/range1986780/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986780/range1986780/1
type: Range
persistent identity: range1986780
display id: range1986780
version: 1
direction: direction/forward
top level: cI+luxR
start: 81
end: 97
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986779
display id: annotation1986779
version: 1
top level: cI+luxR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986779/range1986779/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986779/range1986779/1
type: Range
persistent identity: range1986779
display id: range1986779
version: 1
direction: direction/forward
top level: cI+luxR
start: 71
end: 76
orientation: inline

lux p(R) start
type: SequenceAnnotation
persistent identity: annotation1986778
display id: annotation1986778
version: 1
top level: cI+luxR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986778/range1986778/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986778/range1986778/1
type: Range
persistent identity: range1986778
display id: range1986778
version: 1
direction: direction/forward
top level: cI+luxR
start: 58
end: 58
orientation: inline

OR2 cI
type: SequenceAnnotation
persistent identity: annotation1986777
display id: annotation1986777
version: 1
top level: cI+luxR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986777/range1986777/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986777/range1986777/1
type: Range
persistent identity: range1986777
display id: range1986777
version: 1
direction: direction/forward
top level: cI+luxR
start: 57
end: 73
orientation: inline

BBa_R0065
type: SequenceAnnotation
persistent identity: annotation1986774
display id: annotation1986774
version: 1
top level: cI+luxR
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986774/range1986774/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986774/range1986774/1
type: Range
persistent identity: range1986774
display id: range1986774
version: 1
direction: direction/forward
top level: cI+luxR
start: 1
end: 97
orientation: inline

BBa_K180007
type: ComponentDefinition
persistent identity: BBa K180007
display id: BBa_K180007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Game of Life - Secondary plasmid [tac pR, LuxI generator]
creator: Richard Wilson, Stephanie Chambers
created: 2009-10-15T11:00:00Z
modified: 2015-05-08T01:11:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _278_
m user id: 0
owner id: 4082
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: iGEM2009 Team:Southampton Game of Life secondary plasmid.
mutable notes: Regulated expression of LuxI (C0161) by TAC pR (K180000), a functional hybrid lac/trp promoter.
mutable provenance: See relevant basic biobrick parts (K180000, B0034, C0161, B0015).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0012
component: BBa_B0010
component: BBa_K180000
component: BBa_B0034
component: BBa_C0161
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_K180000
sequence annotation: BBa_B0034
sequence annotation: BBa_C0161
sequence: https://synbiohub.org/public/igem/BBa_K180007_sequence/1

BBa_B0012
type: Component
persistent identity: component2046435
display id: component2046435
version: 1
top level: BBa_K180007
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component2046433
display id: component2046433
version: 1
top level: BBa_K180007
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_K180000
type: Component
persistent identity: component2046428
display id: component2046428
version: 1
top level: BBa_K180007
access: public
definition: BBa_K180000

BBa_B0034
type: Component
persistent identity: component2046430
display id: component2046430
version: 1
top level: BBa_K180007
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0161
type: Component
persistent identity: component2046432
display id: component2046432
version: 1
top level: BBa_K180007
access: public
definition: https://synbiohub.org/public/igem/BBa_C0161/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2046435
display id: annotation2046435
version: 1
top level: BBa_K180007
location: https://synbiohub.org/public/igem/BBa_K180007/annotation2046435/range2046435/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K180007/annotation2046435/range2046435/1
type: Range
persistent identity: range2046435
display id: range2046435
version: 1
direction: direction/forward
top level: BBa_K180007
start: 2155
end: 2195
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2046433
display id: annotation2046433
version: 1
top level: BBa_K180007
location: https://synbiohub.org/public/igem/BBa_K180007/annotation2046433/range2046433/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K180007/annotation2046433/range2046433/1
type: Range
persistent identity: range2046433
display id: range2046433
version: 1
direction: direction/forward
top level: BBa_K180007
start: 2067
end: 2146
orientation: inline

BBa_K180000
type: SequenceAnnotation
persistent identity: annotation2046428
display id: annotation2046428
version: 1
top level: BBa_K180007
location: https://synbiohub.org/public/igem/BBa_K180007/annotation2046428/range2046428/1
component: BBa_K180000

https://synbiohub.org/public/igem/BBa_K180007/annotation2046428/range2046428/1
type: Range
persistent identity: range2046428
display id: range2046428
version: 1
direction: direction/reverse
top level: BBa_K180007
start: 1
end: 1447
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2046430
display id: annotation2046430
version: 1
top level: BBa_K180007
location: https://synbiohub.org/public/igem/BBa_K180007/annotation2046430/range2046430/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K180007/annotation2046430/range2046430/1
type: Range
persistent identity: range2046430
display id: range2046430
version: 1
direction: direction/reverse
top level: BBa_K180007
start: 1456
end: 1467
orientation: inline

BBa_C0161
type: SequenceAnnotation
persistent identity: annotation2046432
display id: annotation2046432
version: 1
top level: BBa_K180007
location: https://synbiohub.org/public/igem/BBa_K180007/annotation2046432/range2046432/1
component: BBa_C0161

https://synbiohub.org/public/igem/BBa_K180007/annotation2046432/range2046432/1
type: Range
persistent identity: range2046432
display id: range2046432
version: 1
direction: direction/reverse
top level: BBa_K180007
start: 1474
end: 2058
orientation: inline

Cpn60/10
type: ComponentDefinition
persistent identity: BBa K538311
display id: BBa_K538311
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cpn60/10 operon (pBAD)
creator: Bas Stringer
created: 2011-09-07T11:00:00Z
modified: 2015-05-08T01:12:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _704_
m user id: 0
owner id: 9985
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_K538000
component: BBa_K538001
component: BBa_K206000
sequence annotation: BBa_K538000
sequence annotation: BBa_K206000
sequence annotation: BBa_K538001
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K538311_sequence/1

BBa_B0034
type: Component
persistent identity: component2222818
display id: component2222818
version: 1
top level: Cpn60/10
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K538000
type: Component
persistent identity: component2222821
display id: component2222821
version: 1
top level: Cpn60/10
access: public
definition: https://synbiohub.org/public/igem/BBa_K538000/1

BBa_B0034
type: Component
persistent identity: component2222812
display id: component2222812
version: 1
top level: Cpn60/10
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K538001
type: Component
persistent identity: component2222816
display id: component2222816
version: 1
top level: Cpn60/10
access: public
definition: https://synbiohub.org/public/igem/BBa_K538001/1

BBa_K206000
type: Component
persistent identity: component2222810
display id: component2222810
version: 1
top level: Cpn60/10
access: public
definition: pBAD

BBa_K538000
type: SequenceAnnotation
persistent identity: annotation2222821
display id: annotation2222821
version: 1
top level: Cpn60/10
location: https://synbiohub.org/public/igem/BBa_K538311/annotation2222821/range2222821/1
component: BBa_K538000

https://synbiohub.org/public/igem/BBa_K538311/annotation2222821/range2222821/1
type: Range
persistent identity: range2222821
display id: range2222821
version: 1
direction: direction/reverse
top level: Cpn60/10
start: 1833
end: 2129
orientation: inline

BBa_K206000
type: SequenceAnnotation
persistent identity: annotation2222810
display id: annotation2222810
version: 1
top level: Cpn60/10
location: https://synbiohub.org/public/igem/BBa_K538311/annotation2222810/range2222810/1
component: BBa_K206000

https://synbiohub.org/public/igem/BBa_K538311/annotation2222810/range2222810/1
type: Range
persistent identity: range2222810
display id: range2222810
version: 1
direction: direction/reverse
top level: Cpn60/10
start: 1
end: 130
orientation: inline

BBa_K538001
type: SequenceAnnotation
persistent identity: annotation2222816
display id: annotation2222816
version: 1
top level: Cpn60/10
location: https://synbiohub.org/public/igem/BBa_K538311/annotation2222816/range2222816/1
component: BBa_K538001

https://synbiohub.org/public/igem/BBa_K538311/annotation2222816/range2222816/1
type: Range
persistent identity: range2222816
display id: range2222816
version: 1
direction: direction/reverse
top level: Cpn60/10
start: 157
end: 1806
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2222818
display id: annotation2222818
version: 1
top level: Cpn60/10
location: https://synbiohub.org/public/igem/BBa_K538311/annotation2222818/range2222818/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K538311/annotation2222818/range2222818/1
type: Range
persistent identity: range2222818
display id: range2222818
version: 1
direction: direction/reverse
top level: Cpn60/10
start: 1815
end: 1826
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2222812
display id: annotation2222812
version: 1
top level: Cpn60/10
location: https://synbiohub.org/public/igem/BBa_K538311/annotation2222812/range2222812/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K538311/annotation2222812/range2222812/1
type: Range
persistent identity: range2222812
display id: range2222812
version: 1
direction: direction/reverse
top level: Cpn60/10
start: 139
end: 150
orientation: inline

BBa_K831016
type: ComponentDefinition
persistent identity: BBa K831016
display id: BBa_K831016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PxpsR
creator: Paola Reyes Caldas
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:32Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1090_
m user id: 0
owner id: 9964
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Promoter of xpsR (PxpsR) that responds to the Quorum Sensing signal 3 OH-PAME especific of Ralstonia solanacearum.
mutable notes: RFC10 compatible
mutable provenance: Ralstonia solanacearum AW
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: vsrD box
sequence annotation: -10
sequence annotation: phcA box
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_K831016_sequence/1

vsrD box
type: SequenceAnnotation
persistent identity: annotation2200840
display id: annotation2200840
version: 1
top level: BBa_K831016
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K831016/annotation2200840/range2200840/1

https://synbiohub.org/public/igem/BBa_K831016/annotation2200840/range2200840/1
type: Range
persistent identity: range2200840
display id: range2200840
version: 1
direction: direction/forward
top level: BBa_K831016
start: 18
end: 39
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2200712
display id: annotation2200712
version: 1
top level: BBa_K831016
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K831016/annotation2200712/range2200712/1

https://synbiohub.org/public/igem/BBa_K831016/annotation2200712/range2200712/1
type: Range
persistent identity: range2200712
display id: range2200712
version: 1
direction: direction/forward
top level: BBa_K831016
start: 169
end: 175
orientation: inline

phcA box
type: SequenceAnnotation
persistent identity: annotation2200776
display id: annotation2200776
version: 1
top level: BBa_K831016
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K831016/annotation2200776/range2200776/1

https://synbiohub.org/public/igem/BBa_K831016/annotation2200776/range2200776/1
type: Range
persistent identity: range2200776
display id: range2200776
version: 1
direction: direction/forward
top level: BBa_K831016
start: 153
end: 159
orientation: inline

phcA box
type: SequenceAnnotation
persistent identity: annotation2200744
display id: annotation2200744
version: 1
top level: BBa_K831016
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K831016/annotation2200744/range2200744/1

https://synbiohub.org/public/igem/BBa_K831016/annotation2200744/range2200744/1
type: Range
persistent identity: range2200744
display id: range2200744
version: 1
direction: direction/forward
top level: BBa_K831016
start: 163
end: 176
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2200713
display id: annotation2200713
version: 1
top level: BBa_K831016
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K831016/annotation2200713/range2200713/1

https://synbiohub.org/public/igem/BBa_K831016/annotation2200713/range2200713/1
type: Range
persistent identity: range2200713
display id: range2200713
version: 1
direction: direction/forward
top level: BBa_K831016
start: 146
end: 151
orientation: inline

BBa_K116201
type: ComponentDefinition
persistent identity: BBa K116201
display id: BBa_K116201
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ureD promoter from P mirabilis
creator: Yuan-Hao Lee
created: 2008-10-30T12:00:00Z
modified: 2015-05-08T01:09:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _210_
m user id: 0
owner id: 2716
owning group id: 9
sample status: No part sequence
status: status/Planning
bookmark: false
mutable description: An 328 bp promoter region, which can be assembled with urea binding proteins to activate the downstream gene expression.
mutable notes: pUreD is a urea responsive element that should be turned on by the complex of UreR and urea.
mutable provenance: This is a component of urea sensor cloned from Proteus mirabilis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory

lux pR-3
type: ComponentDefinition
persistent identity: BBa K658006
display id: BBa_K658006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: position 3 mutated promoter lux pR-3 (luxR & HSL regulated)
creator: Yidan Hu
created: 2011-09-30T11:00:00Z
modified: 2015-05-08T01:13:02Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _835_
m user id: 0
owner id: 10207
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The position 3 mutated promoter lux pR-3 is a product of site-directed mutagenesis of promoter lux pR (BBa_R0062). The promoter lux pR is based on the Vibrio fischeri quorum sensing gene promoters. The position 3 mutated promoter lux pR-3 gives a higher expression of downstream genes in an autoinduction system. In another word, the lux pR-3 has a higher efficiency than the lux pR. Similar to promoter lux pR, the expression is up-regulated by the action of LuxR protein combined with the signal molecule HSL.
mutable notes: In the promoter lux pR's sequence, the C residue of position 3 was changed to a T using PCR method.
mutable provenance: The position 3 mutated promoter lux pR-3 is based on promoter lux pR (BBa_R0062).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: -35
sequence annotation: LuxR/HSL
sequence annotation: start
sequence annotation: K658006
sequence: https://synbiohub.org/public/igem/BBa_K658006_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation2148162
display id: annotation2148162
version: 1
top level: lux pR-3
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K658006/annotation2148162/range2148162/1

https://synbiohub.org/public/igem/BBa_K658006/annotation2148162/range2148162/1
type: Range
persistent identity: range2148162
display id: range2148162
version: 1
direction: direction/forward
top level: lux pR-3
start: 42
end: 47
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2148161
display id: annotation2148161
version: 1
top level: lux pR-3
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K658006/annotation2148161/range2148161/1

https://synbiohub.org/public/igem/BBa_K658006/annotation2148161/range2148161/1
type: Range
persistent identity: range2148161
display id: range2148161
version: 1
direction: direction/forward
top level: lux pR-3
start: 20
end: 25
orientation: inline

LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2148166
display id: annotation2148166
version: 1
top level: lux pR-3
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K658006/annotation2148166/range2148166/1

https://synbiohub.org/public/igem/BBa_K658006/annotation2148166/range2148166/1
type: Range
persistent identity: range2148166
display id: range2148166
version: 1
direction: direction/forward
top level: lux pR-3
start: 1
end: 20
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2148163
display id: annotation2148163
version: 1
top level: lux pR-3
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K658006/annotation2148163/range2148163/1

https://synbiohub.org/public/igem/BBa_K658006/annotation2148163/range2148163/1
type: Range
persistent identity: range2148163
display id: range2148163
version: 1
direction: direction/forward
top level: lux pR-3
start: 53
end: 53
orientation: inline

K658006
type: SequenceAnnotation
persistent identity: annotation2148164
display id: annotation2148164
version: 1
top level: lux pR-3
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K658006/annotation2148164/range2148164/1

https://synbiohub.org/public/igem/BBa_K658006/annotation2148164/range2148164/1
type: Range
persistent identity: range2148164
display id: range2148164
version: 1
direction: direction/forward
top level: lux pR-3
start: 1
end: 55
orientation: inline

BBa_K338062
type: ComponentDefinition
persistent identity: BBa K338062
display id: BBa_K338062
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP Reporter Device, Heat-Shock or IPTG Induced
creator: Lucas Hartsough
created: 2010-10-26T11:00:00Z
modified: 2015-05-08T01:12:07Z
discontinued: false
dominant: false
group u list: _454_
m user id: 0
owner id: 6013
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Construct assembled to allow the quantitative analysis of the heat shock/LacI promoter/repressor system, BBa_K338002 . Produces GFP in response to low-temperature heat shock, more-so in the presence of LacI. GFP production is retarded in LacIq cells (or in the presence of +5mM glucose) about 4-6x. See K338002 for more detailed data.
mutable notes: The type of GFP used in this construct happens to have a very long half life. If growing cultures for fluorescence measurements, grow at 30??C - any higher will activate the promoter, causing GFP to be produced.
mutable provenance: The Registry of Standard Biological Parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0015
component: BBa_B0034
component: BBa_E0040
component: BBa_K338002
sequence annotation: BBa_E0040
sequence annotation: BBa_B0015
sequence annotation: BBa_B0034
sequence annotation: BBa_K338002
sequence: https://synbiohub.org/public/igem/BBa_K338062_sequence/1

BBa_B0015
type: Component
persistent identity: component2105145
display id: component2105145
version: 1
top level: BBa_K338062
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0034
type: Component
persistent identity: component2105136
display id: component2105136
version: 1
top level: BBa_K338062
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0040
type: Component
persistent identity: component2105138
display id: component2105138
version: 1
top level: BBa_K338062
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_K338002
type: Component
persistent identity: component2105134
display id: component2105134
version: 1
top level: BBa_K338062
access: public
definition: HSP-LacI

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2105138
display id: annotation2105138
version: 1
top level: BBa_K338062
location: https://synbiohub.org/public/igem/BBa_K338062/annotation2105138/range2105138/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K338062/annotation2105138/range2105138/1
type: Range
persistent identity: range2105138
display id: range2105138
version: 1
direction: direction/reverse
top level: BBa_K338062
start: 208
end: 927
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2105145
display id: annotation2105145
version: 1
top level: BBa_K338062
location: https://synbiohub.org/public/igem/BBa_K338062/annotation2105145/range2105145/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K338062/annotation2105145/range2105145/1
type: Range
persistent identity: range2105145
display id: range2105145
version: 1
direction: direction/reverse
top level: BBa_K338062
start: 936
end: 1064
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2105136
display id: annotation2105136
version: 1
top level: BBa_K338062
location: https://synbiohub.org/public/igem/BBa_K338062/annotation2105136/range2105136/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K338062/annotation2105136/range2105136/1
type: Range
persistent identity: range2105136
display id: range2105136
version: 1
direction: direction/reverse
top level: BBa_K338062
start: 190
end: 201
orientation: inline

BBa_K338002
type: SequenceAnnotation
persistent identity: annotation2105134
display id: annotation2105134
version: 1
top level: BBa_K338062
location: https://synbiohub.org/public/igem/BBa_K338062/annotation2105134/range2105134/1
component: BBa_K338002

https://synbiohub.org/public/igem/BBa_K338062/annotation2105134/range2105134/1
type: Range
persistent identity: range2105134
display id: range2105134
version: 1
direction: direction/reverse
top level: BBa_K338062
start: 1
end: 181
orientation: inline

BBa_J05218
type: ComponentDefinition
persistent identity: BBa J05218
display id: BBa_J05218
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Regulator for R4-cMaf
creator: Alexander Roth
created: 2005-09-07T11:00:00Z
modified: 2015-08-31T04:08:15Z
discontinued: false
dominant: false
group u list: _34_
m user id: 0
owner id: 412
owning group id: 34
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: binding sites for R1-CREBH and R2-YAP7
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J05218_sequence/1

pBAD:CspC
type: ComponentDefinition
persistent identity: BBa K538304
display id: BBa_K538304
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBAD + RBS + CspC
creator: Bas Stringer
created: 2011-09-07T11:00:00Z
modified: 2015-05-08T01:12:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _704_
m user id: 0
owner id: 9985
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K538004
component: BBa_K206000
component: BBa_B0034
sequence annotation: BBa_K538004
sequence annotation: BBa_B0034
sequence annotation: BBa_K206000
sequence: https://synbiohub.org/public/igem/BBa_K538304_sequence/1

BBa_K538004
type: Component
persistent identity: component2134109
display id: component2134109
version: 1
top level: pBAD:CspC
access: public
definition: https://synbiohub.org/public/igem/BBa_K538004/1

BBa_K206000
type: Component
persistent identity: component2134101
display id: component2134101
version: 1
top level: pBAD:CspC
access: public
definition: pBAD

BBa_B0034
type: Component
persistent identity: component2134103
display id: component2134103
version: 1
top level: pBAD:CspC
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K538004
type: SequenceAnnotation
persistent identity: annotation2134109
display id: annotation2134109
version: 1
top level: pBAD:CspC
location: https://synbiohub.org/public/igem/BBa_K538304/annotation2134109/range2134109/1
component: BBa_K538004

https://synbiohub.org/public/igem/BBa_K538304/annotation2134109/range2134109/1
type: Range
persistent identity: range2134109
display id: range2134109
version: 1
direction: direction/reverse
top level: pBAD:CspC
start: 157
end: 351
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2134103
display id: annotation2134103
version: 1
top level: pBAD:CspC
location: https://synbiohub.org/public/igem/BBa_K538304/annotation2134103/range2134103/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K538304/annotation2134103/range2134103/1
type: Range
persistent identity: range2134103
display id: range2134103
version: 1
direction: direction/reverse
top level: pBAD:CspC
start: 139
end: 150
orientation: inline

BBa_K206000
type: SequenceAnnotation
persistent identity: annotation2134101
display id: annotation2134101
version: 1
top level: pBAD:CspC
location: https://synbiohub.org/public/igem/BBa_K538304/annotation2134101/range2134101/1
component: BBa_K206000

https://synbiohub.org/public/igem/BBa_K538304/annotation2134101/range2134101/1
type: Range
persistent identity: range2134101
display id: range2134101
version: 1
direction: direction/reverse
top level: pBAD:CspC
start: 1
end: 130
orientation: inline

BBa_K136001
type: ComponentDefinition
persistent identity: BBa K136001
display id: BBa_K136001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter of flgA gene from E. coli flagella
creator: Cyprien Maisonnier
created: 2008-08-11T11:00:00Z
modified: 2015-05-08T01:10:02Z
discontinued: true
dominant: false
experience: experience/None
group u list: _211_
m user id: 0
owner id: 3012
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: ''fliA'' is a class 2 gene that is part of the construction of the flagella ( ''Ordering Genes in a Flagella Pathway by Analysis of Expression Kinetics from Living Bacteria'' - S. Kalir, J. McClure, K. Pabbaraju, C. Southward, M. Ronen, S. Leibler, M. G. Surette, U. Alon ). Its promoter is consitutively repressed. It is activated by FlhD4C2 and maybe by FliA with different strength ( ''Using a Quantitative Blueprint to Reprogram the Dynamics of the Flagella Gene Network'' - Shiraz Kalir and Uri Alon ). '''Work in progress :''' Deeper quantitative characterization
mutable notes: The primers used to amplify this sequence are : *Forward : GTTTCTTCGAATTCGCGGCCGCTTCTAGAGAGCATATCTCCTCCGCAGGTATCAAAAT *Reverse : GTTTCTTCCTGCAGCGGCCGCTACTAGTAACAGTATCGCGATGATCGCCACGCTACGT
mutable provenance: This part comes from the genome of ''E.coli'' K12 strain MG1655.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: FlhDC binding site 1
sequence annotation: Initiation start for transcription
sequence annotation: FlhDC binding site 2
sequence: https://synbiohub.org/public/igem/BBa_K136001_sequence/1

FlhDC binding site 1
type: SequenceAnnotation
persistent identity: annotation1971568
display id: annotation1971568
version: 1
top level: BBa_K136001
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K136001/annotation1971568/range1971568/1

https://synbiohub.org/public/igem/BBa_K136001/annotation1971568/range1971568/1
type: Range
persistent identity: range1971568
display id: range1971568
version: 1
direction: direction/forward
top level: BBa_K136001
start: 63
end: 78
orientation: inline

Initiation start for transcription
type: SequenceAnnotation
persistent identity: annotation1971570
display id: annotation1971570
version: 1
top level: BBa_K136001
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K136001/annotation1971570/range1971570/1

https://synbiohub.org/public/igem/BBa_K136001/annotation1971570/range1971570/1
type: Range
persistent identity: range1971570
display id: range1971570
version: 1
direction: direction/forward
top level: BBa_K136001
start: 138
end: 138
orientation: inline

FlhDC binding site 2
type: SequenceAnnotation
persistent identity: annotation1971569
display id: annotation1971569
version: 1
top level: BBa_K136001
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K136001/annotation1971569/range1971569/1

https://synbiohub.org/public/igem/BBa_K136001/annotation1971569/range1971569/1
type: Range
persistent identity: range1971569
display id: range1971569
version: 1
direction: direction/forward
top level: BBa_K136001
start: 69
end: 104
orientation: inline

pBAD:Cpn60
type: ComponentDefinition
persistent identity: BBa K538301
display id: BBa_K538301
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBAD + RBS + Cpn60
creator: Bas Stringer
created: 2011-09-07T11:00:00Z
modified: 2015-05-08T01:12:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _704_
m user id: 0
owner id: 9985
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K538001
component: BBa_K206000
component: BBa_B0034
sequence annotation: BBa_K538001
sequence annotation: BBa_K206000
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K538301_sequence/1

BBa_K538001
type: Component
persistent identity: component2219887
display id: component2219887
version: 1
top level: pBAD:Cpn60
access: public
definition: https://synbiohub.org/public/igem/BBa_K538001/1

BBa_K206000
type: Component
persistent identity: component2219881
display id: component2219881
version: 1
top level: pBAD:Cpn60
access: public
definition: pBAD

BBa_B0034
type: Component
persistent identity: component2219883
display id: component2219883
version: 1
top level: pBAD:Cpn60
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K538001
type: SequenceAnnotation
persistent identity: annotation2219887
display id: annotation2219887
version: 1
top level: pBAD:Cpn60
location: https://synbiohub.org/public/igem/BBa_K538301/annotation2219887/range2219887/1
component: BBa_K538001

https://synbiohub.org/public/igem/BBa_K538301/annotation2219887/range2219887/1
type: Range
persistent identity: range2219887
display id: range2219887
version: 1
direction: direction/reverse
top level: pBAD:Cpn60
start: 157
end: 1806
orientation: inline

BBa_K206000
type: SequenceAnnotation
persistent identity: annotation2219881
display id: annotation2219881
version: 1
top level: pBAD:Cpn60
location: https://synbiohub.org/public/igem/BBa_K538301/annotation2219881/range2219881/1
component: BBa_K206000

https://synbiohub.org/public/igem/BBa_K538301/annotation2219881/range2219881/1
type: Range
persistent identity: range2219881
display id: range2219881
version: 1
direction: direction/reverse
top level: pBAD:Cpn60
start: 1
end: 130
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2219883
display id: annotation2219883
version: 1
top level: pBAD:Cpn60
location: https://synbiohub.org/public/igem/BBa_K538301/annotation2219883/range2219883/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K538301/annotation2219883/range2219883/1
type: Range
persistent identity: range2219883
display id: range2219883
version: 1
direction: direction/reverse
top level: pBAD:Cpn60
start: 139
end: 150
orientation: inline

RhlR+C4
type: ComponentDefinition
persistent identity: BBa R0071
display id: BBa_R0071
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (RhlR & C4-HSL regulated)
creator: Ronny Krashinsky
created: 2004-01-24T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Promoter activated by RhlR in concert with C4-HSL. C4-HSL is produced by RhlI, and acts as a quorum-sensing autoinducer. This is the natural sequence taken from Pseudomonas aeruginosa. Crosstalk: this promoter is also activated at a low level by LasR with its associated HSL.
mutable notes: The -10 and -35 sites are labeled as identified in [Pearson97]. The part begins with the RhlR binding site, and (rather arbitrarily) ends with the first transcribed base (+1).
mutable provenance: Pseudomonas aeruginosa rhlAB promoter
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: RhlR
sequence annotation: start
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0071_sequence/1

RhlR
type: SequenceAnnotation
persistent identity: annotation297102
display id: annotation297102
version: 1
top level: RhlR+C4
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0071/annotation297102/range297102/1

https://synbiohub.org/public/igem/BBa_R0071/annotation297102/range297102/1
type: Range
persistent identity: range297102
display id: range297102
version: 1
direction: direction/forward
top level: RhlR+C4
start: 1
end: 20
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation297105
display id: annotation297105
version: 1
top level: RhlR+C4
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_R0071/annotation297105/range297105/1

https://synbiohub.org/public/igem/BBa_R0071/annotation297105/range297105/1
type: Range
persistent identity: range297105
display id: range297105
version: 1
direction: direction/forward
top level: RhlR+C4
start: 53
end: 53
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation297103
display id: annotation297103
version: 1
top level: RhlR+C4
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0071/annotation297103/range297103/1

https://synbiohub.org/public/igem/BBa_R0071/annotation297103/range297103/1
type: Range
persistent identity: range297103
display id: range297103
version: 1
direction: direction/forward
top level: RhlR+C4
start: 19
end: 24
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation297104
display id: annotation297104
version: 1
top level: RhlR+C4
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0071/annotation297104/range297104/1

https://synbiohub.org/public/igem/BBa_R0071/annotation297104/range297104/1
type: Range
persistent identity: range297104
display id: range297104
version: 1
direction: direction/forward
top level: RhlR+C4
start: 42
end: 47
orientation: inline

Prm +
type: ComponentDefinition
persistent identity: BBa I12007
display id: BBa_I12007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Modified lambda Prm promoter (OR-3 obliterated)
creator: Hans
created: 2004-07-14T11:00:00Z
modified: 2015-08-31T04:07:31Z
discontinued: false
dominant: true
experience: experience/None
group u list: _3_
m user id: 0
owner id: 103
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Lambda Prm promoter modified to be activated but not repressed by the lambda repressor (cI)
mutable notes: In wild type lambda phage, the OR3 site (-27 to -11) reads "tatcccttgcggtgata" on the sense strand of DNA. To prevent lambda repressor (cI) from binding to this site, the 4th through 10th nt of OR3 were replaced by the 7 nt between OR2 and OR1, "aaatagt" (-57 to -51), which in effect mutates 5 nt of the promoter. These nt were chosen to be about halfway between the -10 and -35 boxes of the promoter. Furthermore, since these nt already act as a spacer in wild-type phage, it is hoped they will not create undesired interactions.
mutable provenance: Shih and Gussin (1983)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: mutate to obliterate OR3
sequence annotation: -10
sequence annotation: -35
sequence annotation: OR2
sequence annotation: OR1
sequence: https://synbiohub.org/public/igem/BBa_I12007_sequence/1

mutate to obliterate OR3
type: SequenceAnnotation
persistent identity: annotation937704
display id: annotation937704
version: 1
top level: Prm +
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_I12007/annotation937704/range937704/1

https://synbiohub.org/public/igem/BBa_I12007/annotation937704/range937704/1
type: Range
persistent identity: range937704
display id: range937704
version: 1
direction: direction/forward
top level: Prm +
start: 59
end: 65
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation937705
display id: annotation937705
version: 1
top level: Prm +
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I12007/annotation937705/range937705/1

https://synbiohub.org/public/igem/BBa_I12007/annotation937705/range937705/1
type: Range
persistent identity: range937705
display id: range937705
version: 1
direction: direction/forward
top level: Prm +
start: 71
end: 76
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation937703
display id: annotation937703
version: 1
top level: Prm +
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I12007/annotation937703/range937703/1

https://synbiohub.org/public/igem/BBa_I12007/annotation937703/range937703/1
type: Range
persistent identity: range937703
display id: range937703
version: 1
direction: direction/forward
top level: Prm +
start: 48
end: 53
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation937702
display id: annotation937702
version: 1
top level: Prm +
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12007/annotation937702/range937702/1

https://synbiohub.org/public/igem/BBa_I12007/annotation937702/range937702/1
type: Range
persistent identity: range937702
display id: range937702
version: 1
direction: direction/forward
top level: Prm +
start: 33
end: 49
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation937701
display id: annotation937701
version: 1
top level: Prm +
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12007/annotation937701/range937701/1

https://synbiohub.org/public/igem/BBa_I12007/annotation937701/range937701/1
type: Range
persistent identity: range937701
display id: range937701
version: 1
direction: direction/forward
top level: Prm +
start: 9
end: 25
orientation: inline

BBa_K091146
type: ComponentDefinition
persistent identity: BBa K091146
display id: BBa_K091146
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLas/Lux Hybrid Promoter
creator: Erin Feeney
created: 2008-07-16T11:00:00Z
modified: 2015-05-08T01:08:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _191_
m user id: 0
owner id: 3067
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: K091146 is a modified version of the pLas' promoter (BBa_K091117) containing the addition of a luxR binding site between the -35 and -10 regions. This promoter is expected to be activated in the presence of PAI-1 and LasR and repressed in the presence of AI-1 and LuxR.
mutable notes: The Lux box was placed adjacent to the LasR binding site with the aim of minimizing cross-talk in the presence of both PAI-1 and AI-1; since the LasR site should have a higher affinity, we hope that the binding of PAI-1 will competitively exclude the binding of AI-1.
mutable provenance: The LuxR binding site is from BBa_R0062 and the pLas' promoter is from the region upstream of the LasI gene in Pseudomonas aeruginosa.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: LuxR
sequence annotation: LasR
sequence annotation: Transcriptional Start
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_K091146_sequence/1

LuxR
type: SequenceAnnotation
persistent identity: annotation1967355
display id: annotation1967355
version: 1
top level: BBa_K091146
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K091146/annotation1967355/range1967355/1

https://synbiohub.org/public/igem/BBa_K091146/annotation1967355/range1967355/1
type: Range
persistent identity: range1967355
display id: range1967355
version: 1
direction: direction/forward
top level: BBa_K091146
start: 94
end: 113
orientation: inline

LasR
type: SequenceAnnotation
persistent identity: annotation1967354
display id: annotation1967354
version: 1
top level: BBa_K091146
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K091146/annotation1967354/range1967354/1

https://synbiohub.org/public/igem/BBa_K091146/annotation1967354/range1967354/1
type: Range
persistent identity: range1967354
display id: range1967354
version: 1
direction: direction/forward
top level: BBa_K091146
start: 75
end: 93
orientation: inline

Transcriptional Start
type: SequenceAnnotation
persistent identity: annotation1967358
display id: annotation1967358
version: 1
top level: BBa_K091146
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K091146/annotation1967358/range1967358/1

https://synbiohub.org/public/igem/BBa_K091146/annotation1967358/range1967358/1
type: Range
persistent identity: range1967358
display id: range1967358
version: 1
direction: direction/forward
top level: BBa_K091146
start: 125
end: 125
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1967357
display id: annotation1967357
version: 1
top level: BBa_K091146
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K091146/annotation1967357/range1967357/1

https://synbiohub.org/public/igem/BBa_K091146/annotation1967357/range1967357/1
type: Range
persistent identity: range1967357
display id: range1967357
version: 1
direction: direction/forward
top level: BBa_K091146
start: 86
end: 91
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1967356
display id: annotation1967356
version: 1
top level: BBa_K091146
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K091146/annotation1967356/range1967356/1

https://synbiohub.org/public/igem/BBa_K091146/annotation1967356/range1967356/1
type: Range
persistent identity: range1967356
display id: range1967356
version: 1
direction: direction/forward
top level: BBa_K091146
start: 113
end: 118
orientation: inline

BBa_K1700000
type: ComponentDefinition
persistent identity: BBa K1700000
display id: BBa_K1700000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Use toehold to regulate GFP
creator: Meixi Liu
created: 2015-09-16T11:00:00Z
modified: 2015-09-17T09:57:13Z
discontinued: false
dominant: false
group u list: _2118_
m user id: 28512
owner id: 28512
owning group id: 9
status: status/Planning
bookmark: false
mutable description: The structure of toehold switch is similar to hairpin, except it has a loop at the top as ???toehold???. Toehold switch functions as riboregulator through linear-linear interaction between RNAs. When target RNA appears, it will bind one of the toehold switch stems and open the loop, exposing the RBS. Toehold switch systems are composed of two RNA strands referred to as the switch and trigger. The switch RNA contains the coding sequence of the gene being regulated. Upstream of this coding sequence is a hairpin-based processing module containing both a strong RBS and a start codon that is followed by a common 21 nt linker sequence coding for low-molecular-weight amino acids added to the N terminus of the gene of interest. A single-stranded toehold sequence at the 50 end of the hairpin module provides the initial binding site for the trigger RNA strand. This trigger molecule contains an extended single-stranded region that completes a branch migration process with the hairpin to expose the RBS and start codon, thereby initiating translation of the gene of interest. The trigger RNA of our toehold is miRNA-144.When adding it to the system, GFP can be expressed.
mutable notes: Toehold can be open by trigger RNA, thus letting GFP express.
mutable provenance: We synthesis the part.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: ATG start codon
sequence annotation: GFP coding sequence
sequence annotation: toehold
sequence annotation: TAA stop codon
sequence: https://synbiohub.org/public/igem/BBa_K1700000_sequence/1

ATG start codon
type: SequenceAnnotation
persistent identity: annotation2467629
display id: annotation2467629
version: 1
top level: BBa_K1700000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1700000/annotation2467629/range2467629/1

https://synbiohub.org/public/igem/BBa_K1700000/annotation2467629/range2467629/1
type: Range
persistent identity: range2467629
display id: range2467629
version: 1
direction: direction/forward
top level: BBa_K1700000
start: 156
end: 158
orientation: inline

GFP coding sequence
type: SequenceAnnotation
persistent identity: annotation2467632
display id: annotation2467632
version: 1
top level: BBa_K1700000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1700000/annotation2467632/range2467632/1

https://synbiohub.org/public/igem/BBa_K1700000/annotation2467632/range2467632/1
type: Range
persistent identity: range2467632
display id: range2467632
version: 1
direction: direction/forward
top level: BBa_K1700000
start: 101
end: 820
orientation: inline

toehold
type: SequenceAnnotation
persistent identity: annotation2467606
display id: annotation2467606
version: 1
top level: BBa_K1700000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1700000/annotation2467606/range2467606/1

https://synbiohub.org/public/igem/BBa_K1700000/annotation2467606/range2467606/1
type: Range
persistent identity: range2467606
display id: range2467606
version: 1
direction: direction/forward
top level: BBa_K1700000
start: 1
end: 100
orientation: inline

TAA stop codon
type: SequenceAnnotation
persistent identity: annotation2467633
display id: annotation2467633
version: 1
top level: BBa_K1700000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1700000/annotation2467633/range2467633/1

https://synbiohub.org/public/igem/BBa_K1700000/annotation2467633/range2467633/1
type: Range
persistent identity: range2467633
display id: range2467633
version: 1
direction: direction/forward
top level: BBa_K1700000
start: 818
end: 820
orientation: inline

BBa_K180004
type: ComponentDefinition
persistent identity: BBa K180004
display id: BBa_K180004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Game of Life - Primary plasmid
creator: Richard Wilson, Stephanie Chambers
created: 2009-10-15T11:00:00Z
modified: 2015-05-08T01:11:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _278_
m user id: 0
owner id: 4082
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: Composite part, consisting of BBa_K180005 and BBa_K180006, but with BBa_K180005 reading along reverse strand, and BBa_K180006 reading along the forward strand.
mutable notes: BBa_K180005 reading along reverse strand, and BBa_K180006 reading along the forward strand to ensure the constitutive promoter BBa_J23119 (present in BBa_K180005) has no effect over LacI or GFP-LVA expression in BBa_K180006.
mutable provenance: See relevant biobrick component parts (BBa_K180005 and BBa_K180006)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: conserved
sequence annotation: -10
sequence annotation: K180005
sequence annotation: C0012
sequence annotation: -35
sequence annotation: B0034
sequence annotation: luxR
sequence annotation: B0015
sequence annotation: LVA
sequence annotation: C0062
sequence annotation: R0062
sequence annotation: K180006
sequence annotation: lacI
sequence annotation: J23119
sequence annotation: K180001
sequence annotation: GFPmut3b
sequence annotation: LuxR/HSL
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K180004_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation2058526
display id: annotation2058526
version: 1
top level: BBa_K180004
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K180004/annotation2058526/range2058526/1

https://synbiohub.org/public/igem/BBa_K180004/annotation2058526/range2058526/1
type: Range
persistent identity: range2058526
display id: range2058526
version: 1
direction: direction/reverse
top level: BBa_K180004
start: 929
end: 932
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2058543
display id: annotation2058543
version: 1
top level: BBa_K180004
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K180004/annotation2058543/range2058543/1

https://synbiohub.org/public/igem/BBa_K180004/annotation2058543/range2058543/1
type: Range
persistent identity: range2058543
display id: range2058543
version: 1
direction: direction/forward
top level: BBa_K180004
start: 2234
end: 2237
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2058533
display id: annotation2058533
version: 1
top level: BBa_K180004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K180004/annotation2058533/range2058533/1

https://synbiohub.org/public/igem/BBa_K180004/annotation2058533/range2058533/1
type: Range
persistent identity: range2058533
display id: range2058533
version: 1
direction: direction/forward
top level: BBa_K180004
start: 1029
end: 1034
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2058528
display id: annotation2058528
version: 1
top level: BBa_K180004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K180004/annotation2058528/range2058528/1

https://synbiohub.org/public/igem/BBa_K180004/annotation2058528/range2058528/1
type: Range
persistent identity: range2058528
display id: range2058528
version: 1
direction: direction/reverse
top level: BBa_K180004
start: 951
end: 956
orientation: inline

K180005
type: SequenceAnnotation
persistent identity: annotation2058521
display id: annotation2058521
version: 1
top level: BBa_K180004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180004/annotation2058521/range2058521/1

https://synbiohub.org/public/igem/BBa_K180004/annotation2058521/range2058521/1
type: Range
persistent identity: range2058521
display id: range2058521
version: 1
direction: direction/reverse
top level: BBa_K180004
start: 1
end: 979
orientation: inline

C0012
type: SequenceAnnotation
persistent identity: annotation2058535
display id: annotation2058535
version: 1
top level: BBa_K180004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180004/annotation2058535/range2058535/1

https://synbiohub.org/public/igem/BBa_K180004/annotation2058535/range2058535/1
type: Range
persistent identity: range2058535
display id: range2058535
version: 1
direction: direction/forward
top level: BBa_K180004
start: 1069
end: 2196
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2058530
display id: annotation2058530
version: 1
top level: BBa_K180004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K180004/annotation2058530/range2058530/1

https://synbiohub.org/public/igem/BBa_K180004/annotation2058530/range2058530/1
type: Range
persistent identity: range2058530
display id: range2058530
version: 1
direction: direction/reverse
top level: BBa_K180004
start: 974
end: 979
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2058525
display id: annotation2058525
version: 1
top level: BBa_K180004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180004/annotation2058525/range2058525/1

https://synbiohub.org/public/igem/BBa_K180004/annotation2058525/range2058525/1
type: Range
persistent identity: range2058525
display id: range2058525
version: 1
direction: direction/reverse
top level: BBa_K180004
start: 925
end: 936
orientation: inline

luxR
type: SequenceAnnotation
persistent identity: annotation2058524
display id: annotation2058524
version: 1
top level: BBa_K180004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K180004/annotation2058524/range2058524/1

https://synbiohub.org/public/igem/BBa_K180004/annotation2058524/range2058524/1
type: Range
persistent identity: range2058524
display id: range2058524
version: 1
direction: direction/reverse
top level: BBa_K180004
start: 163
end: 912
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2058522
display id: annotation2058522
version: 1
top level: BBa_K180004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180004/annotation2058522/range2058522/1

https://synbiohub.org/public/igem/BBa_K180004/annotation2058522/range2058522/1
type: Range
persistent identity: range2058522
display id: range2058522
version: 1
direction: direction/reverse
top level: BBa_K180004
start: 1
end: 129
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2058538
display id: annotation2058538
version: 1
top level: BBa_K180004
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K180004/annotation2058538/range2058538/1

https://synbiohub.org/public/igem/BBa_K180004/annotation2058538/range2058538/1
type: Range
persistent identity: range2058538
display id: range2058538
version: 1
direction: direction/forward
top level: BBa_K180004
start: 1055
end: 1058
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation2058544
display id: annotation2058544
version: 1
top level: BBa_K180004
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K180004/annotation2058544/range2058544/1

https://synbiohub.org/public/igem/BBa_K180004/annotation2058544/range2058544/1
type: Range
persistent identity: range2058544
display id: range2058544
version: 1
direction: direction/forward
top level: BBa_K180004
start: 2968
end: 3001
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2058545
display id: annotation2058545
version: 1
top level: BBa_K180004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180004/annotation2058545/range2058545/1

https://synbiohub.org/public/igem/BBa_K180004/annotation2058545/range2058545/1
type: Range
persistent identity: range2058545
display id: range2058545
version: 1
direction: direction/forward
top level: BBa_K180004
start: 3010
end: 3138
orientation: inline

C0062
type: SequenceAnnotation
persistent identity: annotation2058523
display id: annotation2058523
version: 1
top level: BBa_K180004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180004/annotation2058523/range2058523/1

https://synbiohub.org/public/igem/BBa_K180004/annotation2058523/range2058523/1
type: Range
persistent identity: range2058523
display id: range2058523
version: 1
direction: direction/reverse
top level: BBa_K180004
start: 163
end: 918
orientation: inline

R0062
type: SequenceAnnotation
persistent identity: annotation2058531
display id: annotation2058531
version: 1
top level: BBa_K180004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180004/annotation2058531/range2058531/1

https://synbiohub.org/public/igem/BBa_K180004/annotation2058531/range2058531/1
type: Range
persistent identity: range2058531
display id: range2058531
version: 1
direction: direction/forward
top level: BBa_K180004
start: 988
end: 1042
orientation: inline

K180006
type: SequenceAnnotation
persistent identity: annotation2058532
display id: annotation2058532
version: 1
top level: BBa_K180004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180004/annotation2058532/range2058532/1

https://synbiohub.org/public/igem/BBa_K180004/annotation2058532/range2058532/1
type: Range
persistent identity: range2058532
display id: range2058532
version: 1
direction: direction/forward
top level: BBa_K180004
start: 988
end: 3138
orientation: inline

lacI
type: SequenceAnnotation
persistent identity: annotation2058536
display id: annotation2058536
version: 1
top level: BBa_K180004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K180004/annotation2058536/range2058536/1

https://synbiohub.org/public/igem/BBa_K180004/annotation2058536/range2058536/1
type: Range
persistent identity: range2058536
display id: range2058536
version: 1
direction: direction/forward
top level: BBa_K180004
start: 1069
end: 2157
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation2058539
display id: annotation2058539
version: 1
top level: BBa_K180004
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K180004/annotation2058539/range2058539/1

https://synbiohub.org/public/igem/BBa_K180004/annotation2058539/range2058539/1
type: Range
persistent identity: range2058539
display id: range2058539
version: 1
direction: direction/forward
top level: BBa_K180004
start: 2158
end: 2196
orientation: inline

J23119
type: SequenceAnnotation
persistent identity: annotation2058527
display id: annotation2058527
version: 1
top level: BBa_K180004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180004/annotation2058527/range2058527/1

https://synbiohub.org/public/igem/BBa_K180004/annotation2058527/range2058527/1
type: Range
persistent identity: range2058527
display id: range2058527
version: 1
direction: direction/reverse
top level: BBa_K180004
start: 945
end: 979
orientation: inline

K180001
type: SequenceAnnotation
persistent identity: annotation2058541
display id: annotation2058541
version: 1
top level: BBa_K180004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180004/annotation2058541/range2058541/1

https://synbiohub.org/public/igem/BBa_K180004/annotation2058541/range2058541/1
type: Range
persistent identity: range2058541
display id: range2058541
version: 1
direction: direction/forward
top level: BBa_K180004
start: 2248
end: 3001
orientation: inline

GFPmut3b
type: SequenceAnnotation
persistent identity: annotation2058540
display id: annotation2058540
version: 1
top level: BBa_K180004
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K180004/annotation2058540/range2058540/1

https://synbiohub.org/public/igem/BBa_K180004/annotation2058540/range2058540/1
type: Range
persistent identity: range2058540
display id: range2058540
version: 1
direction: direction/forward
top level: BBa_K180004
start: 2248
end: 2967
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2058537
display id: annotation2058537
version: 1
top level: BBa_K180004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180004/annotation2058537/range2058537/1

https://synbiohub.org/public/igem/BBa_K180004/annotation2058537/range2058537/1
type: Range
persistent identity: range2058537
display id: range2058537
version: 1
direction: direction/forward
top level: BBa_K180004
start: 1051
end: 1062
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2058542
display id: annotation2058542
version: 1
top level: BBa_K180004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180004/annotation2058542/range2058542/1

https://synbiohub.org/public/igem/BBa_K180004/annotation2058542/range2058542/1
type: Range
persistent identity: range2058542
display id: range2058542
version: 1
direction: direction/forward
top level: BBa_K180004
start: 2230
end: 2241
orientation: inline

LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2058529
display id: annotation2058529
version: 1
top level: BBa_K180004
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K180004/annotation2058529/range2058529/1

https://synbiohub.org/public/igem/BBa_K180004/annotation2058529/range2058529/1
type: Range
persistent identity: range2058529
display id: range2058529
version: 1
direction: direction/forward
top level: BBa_K180004
start: 988
end: 1007
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2058534
display id: annotation2058534
version: 1
top level: BBa_K180004
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K180004/annotation2058534/range2058534/1

https://synbiohub.org/public/igem/BBa_K180004/annotation2058534/range2058534/1
type: Range
persistent identity: range2058534
display id: range2058534
version: 1
direction: direction/forward
top level: BBa_K180004
start: 1040
end: 1040
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2058546
display id: annotation2058546
version: 1
top level: BBa_K180004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K180004/annotation2058546/range2058546/1

https://synbiohub.org/public/igem/BBa_K180004/annotation2058546/range2058546/1
type: Range
persistent identity: range2058546
display id: range2058546
version: 1
direction: direction/forward
top level: BBa_K180004
start: 1007
end: 1012
orientation: inline

BBa_K116001
type: ComponentDefinition
persistent identity: BBa K116001
display id: BBa_K116001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: nhaA promoter, that can be regulated by pH and nhaR protein.
creator: Chih-Hsien Yang
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:09:32Z
discontinued: false
dominant: true
group u list: _210_
m user id: 0
owner id: 592
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: It's E.coli K12 sodium/proton anti-transport promoter. It can be a pH sensor. We test pH senses in different pH value and Sodium concentration.
mutable notes: It should contain NhaR binding site.
mutable provenance: E.coli K12 MG1655 genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K116001_sequence/1

BBa_K1660007
type: ComponentDefinition
persistent identity: BBa K1660007
display id: BBa_K1660007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PompR-6N-gp35-Terminator
creator: Dai Yuanyi
created: 2015-09-07T11:00:00Z
modified: 2015-09-08T12:46:10Z
discontinued: false
dominant: false
group u list: _2078_
m user id: 26933
owner id: 26933
owning group id: 9
status: status/Planning
bookmark: false
mutable description: PompR is a OmpR-controlled promoter which can be positively regulated by phosphorylated OmpR. This promoter is taken from the upstream region of ompC. Phosphorylated OmpR binds to the three operator sites and activates transcription. We insert PompR(BBa_R0082) sequences into the upstream region of our target gene gp35 through restriction-ligation method, which together is connected to vector PSB1C3 afterwards, and therefore we are able to regulate the expression of in through the control of light. Bxb1 gp35 is a serine integrase in Mycobacterium phage Bxb1. This intergrase can exclusively catalyze site-specific recombination. The dismiss of light singal can drive the expression of integrase, which alone can set a DNA sequence(there it means the promoter) flanked by oppositional attB and attP sites, thereby produce an inverted sequence flanked by attL and attR sites. As a consequence, the promotor moves on to express the toxic protein instead of the attractant. We insert the gp35 gene into the downstream region of the PompR promotor by means of 3A synthesis so that the expression of integrase can be regulated by light. In addition, we add a 6N promotor between PompR and gp35 to decrease basal integrase expression above flipping threshold level which could lower the efficiency of int translation. Meanwhile, we insert the recombined sites of attB and attP into the flanking sequences of our constitutive promotor (BBa_J23110 ) and so far we accomplish our reverse promotor system.
mutable notes: we add a 6N promotor between PompR and gp35 to decrease basal integrase expression above flipping threshold level which could lower the efficiency of int translation.
mutable provenance: It comes from the register.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0082
component: BBa_K1433008
sequence annotation: BBa_K1433008
sequence annotation: BBa_R0082
sequence: https://synbiohub.org/public/igem/BBa_K1660007_sequence/1

BBa_R0082
type: Component
persistent identity: component2446518
display id: component2446518
version: 1
top level: BBa_K1660007
access: public
definition: OmpR

BBa_K1433008
type: Component
persistent identity: component2446523
display id: component2446523
version: 1
top level: BBa_K1660007
access: public
definition: https://synbiohub.org/public/igem/BBa_K1433008/1

BBa_K1433008
type: SequenceAnnotation
persistent identity: annotation2446523
display id: annotation2446523
version: 1
top level: BBa_K1660007
location: https://synbiohub.org/public/igem/BBa_K1660007/annotation2446523/range2446523/1
component: BBa_K1433008

https://synbiohub.org/public/igem/BBa_K1660007/annotation2446523/range2446523/1
type: Range
persistent identity: range2446523
display id: range2446523
version: 1
direction: direction/reverse
top level: BBa_K1660007
start: 117
end: 1773
orientation: inline

BBa_R0082
type: SequenceAnnotation
persistent identity: annotation2446518
display id: annotation2446518
version: 1
top level: BBa_K1660007
location: https://synbiohub.org/public/igem/BBa_K1660007/annotation2446518/range2446518/1
component: BBa_R0082

https://synbiohub.org/public/igem/BBa_K1660007/annotation2446518/range2446518/1
type: Range
persistent identity: range2446518
display id: range2446518
version: 1
direction: direction/reverse
top level: BBa_K1660007
start: 1
end: 108
orientation: inline

BBa_K180010
type: ComponentDefinition
persistent identity: BBa K180010
display id: BBa_K180010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Rock-paper-scissors - Rock primary plasmid
creator: Richard Wilson, Stephanie Chambers
created: 2009-10-20T11:00:00Z
modified: 2015-05-08T01:11:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _278_
m user id: 0
owner id: 4082
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: Composite part, consisting of BBa_K180011 and BBa_K180012, but with BBa_K180011 reading along reverse strand, and BBa_K180012 reading along the forward strand. Forms the 'rock' primary plasmid in Team:Southampton's rock-paper-scissors project design.
mutable notes: This part has been specified as a basic BioBrick, due to some of the component parts reading in reverse (unable to specify using the component BioBrick format).
mutable provenance: See relevant biobrick component parts (BBa_K180011 and BBa_K180012).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: B0034
sequence annotation: conserved
sequence annotation: lasI
sequence annotation: lacIq
sequence annotation: -10
sequence annotation: K180000
sequence annotation: K180012
sequence annotation: C0171
sequence annotation: J23119
sequence annotation: C0178
sequence annotation: K180011
sequence annotation: rhlR
sequence annotation: -35
sequence annotation: Ptac
sequence annotation: B0015
sequence annotation: mCherry
sequence annotation: K180008
sequence: https://synbiohub.org/public/igem/BBa_K180010_sequence/1

B0034
type: SequenceAnnotation
persistent identity: annotation2058291
display id: annotation2058291
version: 1
top level: BBa_K180010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058291/range2058291/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058291/range2058291/1
type: Range
persistent identity: range2058291
display id: range2058291
version: 1
direction: direction/forward
top level: BBa_K180010
start: 2391
end: 2402
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2058301
display id: annotation2058301
version: 1
top level: BBa_K180010
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058301/range2058301/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058301/range2058301/1
type: Range
persistent identity: range2058301
display id: range2058301
version: 1
direction: direction/forward
top level: BBa_K180010
start: 3129
end: 3132
orientation: inline

lasI
type: SequenceAnnotation
persistent identity: annotation2058314
display id: annotation2058314
version: 1
top level: BBa_K180010
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058314/range2058314/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058314/range2058314/1
type: Range
persistent identity: range2058314
display id: range2058314
version: 1
direction: direction/forward
top level: BBa_K180010
start: 3143
end: 3751
orientation: inline

lacIq
type: SequenceAnnotation
persistent identity: annotation2058308
display id: annotation2058308
version: 1
top level: BBa_K180010
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058308/range2058308/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058308/range2058308/1
type: Range
persistent identity: range2058308
display id: range2058308
version: 1
direction: direction/forward
top level: BBa_K180010
start: 936
end: 2017
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2058306
display id: annotation2058306
version: 1
top level: BBa_K180010
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058306/range2058306/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058306/range2058306/1
type: Range
persistent identity: range2058306
display id: range2058306
version: 1
direction: direction/reverse
top level: BBa_K180010
start: 899
end: 904
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2058300
display id: annotation2058300
version: 1
top level: BBa_K180010
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058300/range2058300/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058300/range2058300/1
type: Range
persistent identity: range2058300
display id: range2058300
version: 1
direction: direction/forward
top level: BBa_K180010
start: 2395
end: 2398
orientation: inline

K180000
type: SequenceAnnotation
persistent identity: annotation2058307
display id: annotation2058307
version: 1
top level: BBa_K180010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058307/range2058307/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058307/range2058307/1
type: Range
persistent identity: range2058307
display id: range2058307
version: 1
direction: direction/forward
top level: BBa_K180010
start: 936
end: 2382
orientation: inline

K180012
type: SequenceAnnotation
persistent identity: annotation2058287
display id: annotation2058287
version: 1
top level: BBa_K180010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058287/range2058287/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058287/range2058287/1
type: Range
persistent identity: range2058287
display id: range2058287
version: 1
direction: direction/forward
top level: BBa_K180010
start: 936
end: 3888
orientation: inline

C0171
type: SequenceAnnotation
persistent identity: annotation2058289
display id: annotation2058289
version: 1
top level: BBa_K180010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058289/range2058289/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058289/range2058289/1
type: Range
persistent identity: range2058289
display id: range2058289
version: 1
direction: direction/reverse
top level: BBa_K180010
start: 138
end: 866
orientation: inline

J23119
type: SequenceAnnotation
persistent identity: annotation2058304
display id: annotation2058304
version: 1
top level: BBa_K180010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058304/range2058304/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058304/range2058304/1
type: Range
persistent identity: range2058304
display id: range2058304
version: 1
direction: direction/reverse
top level: BBa_K180010
start: 893
end: 927
orientation: inline

C0178
type: SequenceAnnotation
persistent identity: annotation2058313
display id: annotation2058313
version: 1
top level: BBa_K180010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058313/range2058313/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058313/range2058313/1
type: Range
persistent identity: range2058313
display id: range2058313
version: 1
direction: direction/forward
top level: BBa_K180010
start: 3143
end: 3751
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2058303
display id: annotation2058303
version: 1
top level: BBa_K180010
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058303/range2058303/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058303/range2058303/1
type: Range
persistent identity: range2058303
display id: range2058303
version: 1
direction: direction/reverse
top level: BBa_K180010
start: 877
end: 880
orientation: inline

K180011
type: SequenceAnnotation
persistent identity: annotation2058286
display id: annotation2058286
version: 1
top level: BBa_K180010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058286/range2058286/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058286/range2058286/1
type: Range
persistent identity: range2058286
display id: range2058286
version: 1
direction: direction/reverse
top level: BBa_K180010
start: 1
end: 927
orientation: inline

rhlR
type: SequenceAnnotation
persistent identity: annotation2058312
display id: annotation2058312
version: 1
top level: BBa_K180010
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058312/range2058312/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058312/range2058312/1
type: Range
persistent identity: range2058312
display id: range2058312
version: 1
direction: direction/reverse
top level: BBa_K180010
start: 138
end: 866
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2058302
display id: annotation2058302
version: 1
top level: BBa_K180010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058302/range2058302/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058302/range2058302/1
type: Range
persistent identity: range2058302
display id: range2058302
version: 1
direction: direction/reverse
top level: BBa_K180010
start: 873
end: 884
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2058305
display id: annotation2058305
version: 1
top level: BBa_K180010
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058305/range2058305/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058305/range2058305/1
type: Range
persistent identity: range2058305
display id: range2058305
version: 1
direction: direction/reverse
top level: BBa_K180010
start: 922
end: 927
orientation: inline

Ptac
type: SequenceAnnotation
persistent identity: annotation2058309
display id: annotation2058309
version: 1
top level: BBa_K180010
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058309/range2058309/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058309/range2058309/1
type: Range
persistent identity: range2058309
display id: range2058309
version: 1
direction: direction/forward
top level: BBa_K180010
start: 2260
end: 2287
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2058292
display id: annotation2058292
version: 1
top level: BBa_K180010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058292/range2058292/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058292/range2058292/1
type: Range
persistent identity: range2058292
display id: range2058292
version: 1
direction: direction/forward
top level: BBa_K180010
start: 3125
end: 3136
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2058315
display id: annotation2058315
version: 1
top level: BBa_K180010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058315/range2058315/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058315/range2058315/1
type: Range
persistent identity: range2058315
display id: range2058315
version: 1
direction: direction/forward
top level: BBa_K180010
start: 3760
end: 3888
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2058288
display id: annotation2058288
version: 1
top level: BBa_K180010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058288/range2058288/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058288/range2058288/1
type: Range
persistent identity: range2058288
display id: range2058288
version: 1
direction: direction/reverse
top level: BBa_K180010
start: 1
end: 129
orientation: inline

mCherry
type: SequenceAnnotation
persistent identity: annotation2058311
display id: annotation2058311
version: 1
top level: BBa_K180010
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058311/range2058311/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058311/range2058311/1
type: Range
persistent identity: range2058311
display id: range2058311
version: 1
direction: direction/forward
top level: BBa_K180010
start: 2409
end: 3116
orientation: inline

K180008
type: SequenceAnnotation
persistent identity: annotation2058310
display id: annotation2058310
version: 1
top level: BBa_K180010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058310/range2058310/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058310/range2058310/1
type: Range
persistent identity: range2058310
display id: range2058310
version: 1
direction: direction/forward
top level: BBa_K180010
start: 2409
end: 3116
orientation: inline

BBa_K389411
type: ComponentDefinition
persistent identity: BBa K389411
display id: BBa_K389411
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: vir promoter + sensitivity tuner + luciferase
creator: Jonas Aretz
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part combines a mutated ''virG'' response regulator from the VirA/G receptor system under the control of a constitutive promoter with a sensitivity tuner before a luciferase gene under the control of a ''vir'' promoter. The sensitivity tuner enhances a signal from a promoter so it enhances the readout luciferase.
mutable notes: sensitivity tuner to enhance the luciferase signal
mutable provenance: * luciferase from pGL4.10luc2 (Promega) * sensitivity tuner part, terminators and constitutive promoter from the partsregistry * ''virG'' was synthesized by Mr. Gene * ''vir'' promoter from ''Agrobacterium tumefaciens'' C58, TI-plasmid
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K389003
component: BBa_K389002
component: BBa_J23110
component: BBa_B0017
component: BBa_K389401
sequence annotation: BBa_K389401
sequence annotation: BBa_K389003
sequence annotation: BBa_K389002
sequence annotation: BBa_J23110
sequence annotation: BBa_B0017
sequence: https://synbiohub.org/public/igem/BBa_K389411_sequence/1

BBa_K389003
type: Component
persistent identity: component2300736
display id: component2300736
version: 1
top level: BBa_K389411
access: public
definition: Pvir

BBa_K389002
type: Component
persistent identity: component2300726
display id: component2300726
version: 1
top level: BBa_K389411
access: public
definition: https://synbiohub.org/public/igem/BBa_K389002/1

BBa_J23110
type: Component
persistent identity: component2300722
display id: component2300722
version: 1
top level: BBa_K389411
access: public
definition: https://synbiohub.org/public/igem/BBa_J23110/1

BBa_B0017
type: Component
persistent identity: component2300731
display id: component2300731
version: 1
top level: BBa_K389411
access: public
definition: https://synbiohub.org/public/igem/BBa_B0017/1

BBa_K389401
type: Component
persistent identity: component2300760
display id: component2300760
version: 1
top level: BBa_K389411
access: public
definition: https://synbiohub.org/public/igem/BBa_K389401/1

BBa_K389401
type: SequenceAnnotation
persistent identity: annotation2300760
display id: annotation2300760
version: 1
top level: BBa_K389411
location: https://synbiohub.org/public/igem/BBa_K389411/annotation2300760/range2300760/1
component: BBa_K389401

https://synbiohub.org/public/igem/BBa_K389411/annotation2300760/range2300760/1
type: Range
persistent identity: range2300760
display id: range2300760
version: 1
direction: direction/reverse
top level: BBa_K389411
start: 1134
end: 4020
orientation: inline

BBa_K389003
type: SequenceAnnotation
persistent identity: annotation2300736
display id: annotation2300736
version: 1
top level: BBa_K389411
location: https://synbiohub.org/public/igem/BBa_K389411/annotation2300736/range2300736/1
component: BBa_K389003

https://synbiohub.org/public/igem/BBa_K389411/annotation2300736/range2300736/1
type: Range
persistent identity: range2300736
display id: range2300736
version: 1
direction: direction/reverse
top level: BBa_K389411
start: 968
end: 1125
orientation: inline

BBa_K389002
type: SequenceAnnotation
persistent identity: annotation2300726
display id: annotation2300726
version: 1
top level: BBa_K389411
location: https://synbiohub.org/public/igem/BBa_K389411/annotation2300726/range2300726/1
component: BBa_K389002

https://synbiohub.org/public/igem/BBa_K389411/annotation2300726/range2300726/1
type: Range
persistent identity: range2300726
display id: range2300726
version: 1
direction: direction/reverse
top level: BBa_K389411
start: 44
end: 783
orientation: inline

BBa_J23110
type: SequenceAnnotation
persistent identity: annotation2300722
display id: annotation2300722
version: 1
top level: BBa_K389411
location: https://synbiohub.org/public/igem/BBa_K389411/annotation2300722/range2300722/1
component: BBa_J23110

https://synbiohub.org/public/igem/BBa_K389411/annotation2300722/range2300722/1
type: Range
persistent identity: range2300722
display id: range2300722
version: 1
direction: direction/reverse
top level: BBa_K389411
start: 1
end: 35
orientation: inline

BBa_B0017
type: SequenceAnnotation
persistent identity: annotation2300731
display id: annotation2300731
version: 1
top level: BBa_K389411
location: https://synbiohub.org/public/igem/BBa_K389411/annotation2300731/range2300731/1
component: BBa_B0017

https://synbiohub.org/public/igem/BBa_K389411/annotation2300731/range2300731/1
type: Range
persistent identity: range2300731
display id: range2300731
version: 1
direction: direction/reverse
top level: BBa_K389411
start: 792
end: 959
orientation: inline

BBa_I741019
type: ComponentDefinition
persistent identity: BBa I741019
display id: BBa_I741019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Right facing promoter (for xylA) controlled by xylR and CRP-cAMP
creator: Lucien Weiss
created: 2007-06-20T11:00:00Z
modified: 2015-08-31T04:08:02Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _143_
m user id: 0
owner id: 1763
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: naturally the promoter to xylA and xylB
mutable notes: removed RBS cut out several xylR activation sites
mutable provenance: ecoli k12 chromosome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: xylAp
sequence annotation: CRP-cAMP transcriptional dual regulator activates transcription
sequence annotation: XylR-Xylose transcriptional activator activates transcription
sequence: https://synbiohub.org/public/igem/BBa_I741019_sequence/1

xylAp
type: SequenceAnnotation
persistent identity: annotation1935191
display id: annotation1935191
version: 1
top level: BBa_I741019
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I741019/annotation1935191/range1935191/1

https://synbiohub.org/public/igem/BBa_I741019/annotation1935191/range1935191/1
type: Range
persistent identity: range1935191
display id: range1935191
version: 1
direction: direction/reverse
top level: BBa_I741019
start: 76
end: 117
orientation: inline

CRP-cAMP transcriptional dual regulator activates transcription
type: SequenceAnnotation
persistent identity: annotation1935193
display id: annotation1935193
version: 1
top level: BBa_I741019
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I741019/annotation1935193/range1935193/1

https://synbiohub.org/public/igem/BBa_I741019/annotation1935193/range1935193/1
type: Range
persistent identity: range1935193
display id: range1935193
version: 1
direction: direction/forward
top level: BBa_I741019
start: 86
end: 107
orientation: inline

XylR-Xylose transcriptional activator activates transcription
type: SequenceAnnotation
persistent identity: annotation1935195
display id: annotation1935195
version: 1
top level: BBa_I741019
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I741019/annotation1935195/range1935195/1

https://synbiohub.org/public/igem/BBa_I741019/annotation1935195/range1935195/1
type: Range
persistent identity: range1935195
display id: range1935195
version: 1
direction: direction/forward
top level: BBa_I741019
start: 69
end: 85
orientation: inline

XylR-Xylose transcriptional activator activates transcription
type: SequenceAnnotation
persistent identity: annotation1935211
display id: annotation1935211
version: 1
top level: BBa_I741019
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I741019/annotation1935211/range1935211/1

https://synbiohub.org/public/igem/BBa_I741019/annotation1935211/range1935211/1
type: Range
persistent identity: range1935211
display id: range1935211
version: 1
direction: direction/reverse
top level: BBa_I741019
start: 48
end: 64
orientation: inline

BBa_J64750
type: ComponentDefinition
persistent identity: BBa J64750
display id: BBa_J64750
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SPI-1 TTSS secretion-linked promoter from Salmonella
creator: Jeffrey J Tabor
created: 2007-05-14T11:00:00Z
modified: 2015-05-08T01:08:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 88
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This promoter is activated when functional SPI-1 type III secretion systems are present and functional in Salmonella typhimurium 1344.
mutable notes: The promoter includes the binding site for the transcription factor InvF. This transcription factor is activated by the SicA chaparone when the secretion system is active.
mutable provenance: This part was cloned from Salmonella typhimurium 1344 genomic DNA.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: start
sequence annotation: wt rbs
sequence: https://synbiohub.org/public/igem/BBa_J64750_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation1932769
display id: annotation1932769
version: 1
top level: BBa_J64750
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J64750/annotation1932769/range1932769/1

https://synbiohub.org/public/igem/BBa_J64750/annotation1932769/range1932769/1
type: Range
persistent identity: range1932769
display id: range1932769
version: 1
direction: direction/forward
top level: BBa_J64750
start: 165
end: 167
orientation: inline

wt rbs
type: SequenceAnnotation
persistent identity: annotation1932768
display id: annotation1932768
version: 1
top level: BBa_J64750
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J64750/annotation1932768/range1932768/1

https://synbiohub.org/public/igem/BBa_J64750/annotation1932768/range1932768/1
type: Range
persistent identity: range1932768
display id: range1932768
version: 1
direction: direction/forward
top level: BBa_J64750
start: 153
end: 158
orientation: inline

BBa_K284003
type: ComponentDefinition
persistent identity: BBa K284003
display id: BBa_K284003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Partial DLD Promoter from Kluyveromyces lactis
creator: Tais Sineiro Herig, Raissa Estrela Curado and Gleidson Silva Teixeira
created: 2009-09-25T11:00:00Z
modified: 2015-05-08T01:11:48Z
discontinued: false
dominant: false
experience: experience/None
group u list: _386_
m user id: 0
owner id: 4968
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: .
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: primer_binding
sequence annotation: TATA-Like
sequence annotation: DLD Promoter
sequence: https://synbiohub.org/public/igem/BBa_K284003_sequence/1

primer_binding
type: SequenceAnnotation
persistent identity: annotation2034976
display id: annotation2034976
version: 1
top level: BBa_K284003
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K284003/annotation2034976/range2034976/1

https://synbiohub.org/public/igem/BBa_K284003/annotation2034976/range2034976/1
type: Range
persistent identity: range2034976
display id: range2034976
version: 1
direction: direction/reverse
top level: BBa_K284003
start: 442
end: 463
orientation: inline

TATA-Like
type: SequenceAnnotation
persistent identity: annotation2034974
display id: annotation2034974
version: 1
top level: BBa_K284003
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K284003/annotation2034974/range2034974/1

https://synbiohub.org/public/igem/BBa_K284003/annotation2034974/range2034974/1
type: Range
persistent identity: range2034974
display id: range2034974
version: 1
direction: direction/forward
top level: BBa_K284003
start: 300
end: 330
orientation: inline

DLD Promoter
type: SequenceAnnotation
persistent identity: annotation2034972
display id: annotation2034972
version: 1
top level: BBa_K284003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K284003/annotation2034972/range2034972/1

https://synbiohub.org/public/igem/BBa_K284003/annotation2034972/range2034972/1
type: Range
persistent identity: range2034972
display id: range2034972
version: 1
direction: direction/forward
top level: BBa_K284003
start: 1
end: 463
orientation: inline

TATA-Like
type: SequenceAnnotation
persistent identity: annotation2034975
display id: annotation2034975
version: 1
top level: BBa_K284003
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K284003/annotation2034975/range2034975/1

https://synbiohub.org/public/igem/BBa_K284003/annotation2034975/range2034975/1
type: Range
persistent identity: range2034975
display id: range2034975
version: 1
direction: direction/forward
top level: BBa_K284003
start: 355
end: 372
orientation: inline

primer_binding
type: SequenceAnnotation
persistent identity: annotation2034973
display id: annotation2034973
version: 1
top level: BBa_K284003
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K284003/annotation2034973/range2034973/1

https://synbiohub.org/public/igem/BBa_K284003/annotation2034973/range2034973/1
type: Range
persistent identity: range2034973
display id: range2034973
version: 1
direction: direction/forward
top level: BBa_K284003
start: 1
end: 22
orientation: inline

BBa_I766200
type: ComponentDefinition
persistent identity: BBa I766200
display id: BBa_I766200
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSte2
creator: Nili Sommovilla
created: 2007-10-21T11:00:00Z
modified: 2015-08-31T04:08:09Z
discontinued: false
dominant: false
group u list: _155_
m user id: 0
owner id: 1931
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: endogenous promoter of Ste2
mutable notes: none.
mutable provenance: from yeast genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I766200_sequence/1

BBa_K091156
type: ComponentDefinition
persistent identity: BBa K091156
display id: BBa_K091156
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLux
creator: Erin Feeney
created: 2008-07-24T11:00:00Z
modified: 2015-05-08T01:08:38Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _191_
m user id: 0
owner id: 3067
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The pLux' promoter is a modified version of BBa_R0062 containing the -35 region TTTAAT in place of TTTACG. Transcriptional activation is expected to occur in the presence of LuxR and AI-1, just as with R0062. The modified -35 region was designed to serve as a control for the evaluation of BBa_K091156, the pLux+ Las- promoter.
mutable notes: This promoter is identical to pLux+ Las- with the exception of the Las box's absence
mutable provenance: This part was adapted from BBa_R0062.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: LuxR
sequence annotation: -35 Region
sequence annotation: -10 Region
sequence: https://synbiohub.org/public/igem/BBa_K091156_sequence/1

LuxR
type: SequenceAnnotation
persistent identity: annotation1968422
display id: annotation1968422
version: 1
top level: BBa_K091156
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K091156/annotation1968422/range1968422/1

https://synbiohub.org/public/igem/BBa_K091156/annotation1968422/range1968422/1
type: Range
persistent identity: range1968422
display id: range1968422
version: 1
direction: direction/forward
top level: BBa_K091156
start: 1
end: 20
orientation: inline

-35 Region
type: SequenceAnnotation
persistent identity: annotation1968420
display id: annotation1968420
version: 1
top level: BBa_K091156
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K091156/annotation1968420/range1968420/1

https://synbiohub.org/public/igem/BBa_K091156/annotation1968420/range1968420/1
type: Range
persistent identity: range1968420
display id: range1968420
version: 1
direction: direction/forward
top level: BBa_K091156
start: 20
end: 25
orientation: inline

-10 Region
type: SequenceAnnotation
persistent identity: annotation1968421
display id: annotation1968421
version: 1
top level: BBa_K091156
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K091156/annotation1968421/range1968421/1

https://synbiohub.org/public/igem/BBa_K091156/annotation1968421/range1968421/1
type: Range
persistent identity: range1968421
display id: range1968421
version: 1
direction: direction/forward
top level: BBa_K091156
start: 42
end: 47
orientation: inline

BBa_K110004
type: ComponentDefinition
persistent identity: BBa K110004
display id: BBa_K110004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Alpha-Cell Promoter Ste3
creator: James DiCarlo
created: 2008-05-29T11:00:00Z
modified: 2015-05-08T01:09:08Z
discontinued: false
dominant: false
experience: experience/None
group u list: _201_
m user id: 0
owner id: 2669
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This promoter was isolated by taking 500bp upstream of the STE3 gene of S. cerevisiae. This promoter will be activated when alpha factor is expressed in an alpha haploid yeast cell.
mutable notes: This sequence was not edited
mutable provenance: SGD
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K110004_sequence/1

BBa_K112405
type: ComponentDefinition
persistent identity: BBa K112405
display id: BBa_K112405
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter for CadA and CadB genes
creator: Christina Brown
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:17Z
discontinued: false
dominant: false
group u list: _224_
m user id: 0
owner id: 3241
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Our microarray experiment showed that this gene was responsive to sound and ultrasound. Therefore, we cloned the promoter region located approximately 250 base pairs before the start codon for the cadB gene.
mutable notes: There was a relatively high basal level of expression of this gene that made this part difficult to incorporate into our lysis device design.
mutable provenance: cloned from E. coli MG1655 genome located upstream of CadA and CadB genes
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K112405_sequence/1

BBa_K332021
type: ComponentDefinition
persistent identity: BBa K332021
display id: BBa_K332021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ptet + RBS + LuxR
creator: Chen-Hsi Tang
created: 2010-10-23T11:00:00Z
modified: 2015-05-08T01:12:05Z
discontinued: false
dominant: false
experience: experience/None
group u list: _452_
m user id: 0
owner id: 7483
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: In this portion of the design, we aim to achieve population control by combining a cell-signaling system (luxR/luxI) and suicidal gene for controlling the number of E.coli. The population controlling function in the engineered E.coli can warn us of high-density E. coli congregations by emitting fluorescent proteins. Green fluorescent light means the Cry circuit is functional and the population density is under control, while red fluorescent gene signals E.coli overpopulation.
mutable notes: (I) Strand C (BBa_ K332021) 1. Strand C is synthesized from three fragments ??? (1) Ptet (2) RBS+LuxR (3) RBS+LuxI+double ter sites. 2. All of them are in the form of plasmids, so we digest them by appropriate restriction enzymes. 3. We first combine the last two fragments by ligation. The procedure is as follows: (1)Add the respective digestion products, vector plasmid, buffer, ligase to ddwater (2)React at 16??C for 30 minutes (3)Inactivate the ligase by incubation it at 80??C for 20 minutes (4)Store the products at 4??C 4. The products of ligation are then transformed into competent cells. The procedure is as follows: (1)2??l plasmids(ligation products) are added to 33??l competent cells (2)Shake gently for one second, and cool it on ice (3)Incubate it in 42??C water bath for 45 seconds (4)Spread the liquid to plate which corresponding antibiotics have been added to (5)37??C overnight 5. The next day we do a colony PCR for the transformed cells and have the PCR products electrophoresis to make sure that the two fragments have been combined correctly. 6. Now we separate the plasmids(RBS+LuxR+RBS+LuxI+double ter sites) from E. Coli, and digest it with restriction enzyme. 7. The first fragment (Ptet) is then combined with the above product by ligation, and transformed into competent cells by the same we have done early. 8. Because the length of Ptet to the remain segment is relatively too short, we cannot confirm whether they have combined correctly by just do an electrophoresis of the colony PCR products. If the electrophoresis result seems like that they have combined, we then send the product to sequencing.
mutable provenance: 1. LuxI protein (BBa_C0061) is the synthase that converts S-adenosylmethionine (SAM) into acyl-homoserine lactone (AHL). When the concentration of AHL reaches a threshold level, it binds to the N-terminal domain of a LuxR protein. Then the LuxR/AHL complex binds the Lux box within PtetR promoter (BBa_C0040), which then activates suicidal gene expression. 2. LuxR (BBa_C0062) is transcribed continuously by the constitutive promoter (BBa_J23106), and later works in conjunction with AHL. This design can control the concentration of LuxR protein in a steady phase, and the concentration of AHL/LuxR complex is only affected by the concentration of LuxI protein (BBa_C0061).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_C0062
component: BBa_B0034
component: BBa_R0040
sequence annotation: BBa_C0062
sequence annotation: BBa_B0034
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_K332021_sequence/1

BBa_C0062
type: Component
persistent identity: component2247626
display id: component2247626
version: 1
top level: BBa_K332021
access: public
definition: https://synbiohub.org/public/igem/BBa_C0062/1

BBa_B0034
type: Component
persistent identity: component2247623
display id: component2247623
version: 1
top level: BBa_K332021
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0040
type: Component
persistent identity: component2247617
display id: component2247617
version: 1
top level: BBa_K332021
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation2247626
display id: annotation2247626
version: 1
top level: BBa_K332021
location: https://synbiohub.org/public/igem/BBa_K332021/annotation2247626/range2247626/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_K332021/annotation2247626/range2247626/1
type: Range
persistent identity: range2247626
display id: range2247626
version: 1
direction: direction/forward
top level: BBa_K332021
start: 81
end: 836
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2247623
display id: annotation2247623
version: 1
top level: BBa_K332021
location: https://synbiohub.org/public/igem/BBa_K332021/annotation2247623/range2247623/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K332021/annotation2247623/range2247623/1
type: Range
persistent identity: range2247623
display id: range2247623
version: 1
direction: direction/reverse
top level: BBa_K332021
start: 63
end: 74
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2247617
display id: annotation2247617
version: 1
top level: BBa_K332021
location: https://synbiohub.org/public/igem/BBa_K332021/annotation2247617/range2247617/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K332021/annotation2247617/range2247617/1
type: Range
persistent identity: range2247617
display id: range2247617
version: 1
direction: direction/forward
top level: BBa_K332021
start: 1
end: 54
orientation: inline

BBa_K136002
type: ComponentDefinition
persistent identity: BBa K136002
display id: BBa_K136002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter of flgB gene from E. coli flagella
creator: Cyprien Maisonnier
created: 2008-08-11T11:00:00Z
modified: 2015-05-08T01:10:02Z
discontinued: true
dominant: false
experience: experience/None
group u list: _211_
m user id: 0
owner id: 3012
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: ''flgB'' is a class 2 gene that is part of the construction of the flagella ( ''Ordering Genes in a Flagella Pathway by Analysis of Expression Kinetics from Living Bacteria'' - S. Kalir, J. McClure, K. Pabbaraju, C. Southward, M. Ronen, S. Leibler, M. G. Surette, U. Alon ). Its promoter is consitutively repressed. It is activated by FlhD4C2 and maybe by FliA with different strength ( ''Using a Quantitative Blueprint to Reprogram the Dynamics of the Flagella Gene Network'' - Shiraz Kalir and Uri Alon ). '''Work in progress :''' Deeper quantitative characterization
mutable notes: The primers used to amplify this sequence are : *Forward : GTTTCTTCGAATTCGCGGCCGCTTCTAGAGACAGTATCGCGATGATCGCCACGCTACG *Reverse : GTTTCTTCCTGCAGCGGCCGCTACTAGTAAGCATATCTCCTCCGCAGGTATCAAAATT
mutable provenance: This part comes from the genome of ''E.coli'' K12 strain MG1655.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: FlhDC Binding site 2
sequence annotation: FlhDC Binding site 1
sequence annotation: Initiation of Transcription
sequence: https://synbiohub.org/public/igem/BBa_K136002_sequence/1

FlhDC Binding site 2
type: SequenceAnnotation
persistent identity: annotation1971572
display id: annotation1971572
version: 1
top level: BBa_K136002
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K136002/annotation1971572/range1971572/1

https://synbiohub.org/public/igem/BBa_K136002/annotation1971572/range1971572/1
type: Range
persistent identity: range1971572
display id: range1971572
version: 1
direction: direction/forward
top level: BBa_K136002
start: 124
end: 139
orientation: inline

FlhDC Binding site 1
type: SequenceAnnotation
persistent identity: annotation1971571
display id: annotation1971571
version: 1
top level: BBa_K136002
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K136002/annotation1971571/range1971571/1

https://synbiohub.org/public/igem/BBa_K136002/annotation1971571/range1971571/1
type: Range
persistent identity: range1971571
display id: range1971571
version: 1
direction: direction/forward
top level: BBa_K136002
start: 98
end: 113
orientation: inline

Initiation of Transcription
type: SequenceAnnotation
persistent identity: annotation1971573
display id: annotation1971573
version: 1
top level: BBa_K136002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K136002/annotation1971573/range1971573/1

https://synbiohub.org/public/igem/BBa_K136002/annotation1971573/range1971573/1
type: Range
persistent identity: range1971573
display id: range1971573
version: 1
direction: direction/forward
top level: BBa_K136002
start: 173
end: 173
orientation: inline

BBa_K1170000
type: ComponentDefinition
persistent identity: BBa K1170000
display id: BBa_K1170000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: asr (acid shock response) promoter
creator: Rohit Satija
created: 2013-09-01T11:00:00Z
modified: 2015-05-08T01:09:34Z
discontinued: false
dominant: false
group u list: _1483_
m user id: 0
owner id: 16731
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: An acid inducible promoter of the acid shock response (asr) gene, extracted from E. coli K12. This part can be used to generate pH based response in any biological system.
mutable notes: The sequence provided in literature indicated a consensus sequence for the Pho Box just before the transcription start site
mutable provenance: This part was extracted from the genome of E. coli K12
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Pho Box
sequence annotation: asr promoter
sequence annotation: TATA box
sequence: https://synbiohub.org/public/igem/BBa_K1170000_sequence/1

Pho Box
type: SequenceAnnotation
persistent identity: annotation2334964
display id: annotation2334964
version: 1
top level: BBa_K1170000
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1170000/annotation2334964/range2334964/1

https://synbiohub.org/public/igem/BBa_K1170000/annotation2334964/range2334964/1
type: Range
persistent identity: range2334964
display id: range2334964
version: 1
direction: direction/forward
top level: BBa_K1170000
start: 114
end: 131
orientation: inline

asr promoter
type: SequenceAnnotation
persistent identity: annotation2334962
display id: annotation2334962
version: 1
top level: BBa_K1170000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1170000/annotation2334962/range2334962/1

https://synbiohub.org/public/igem/BBa_K1170000/annotation2334962/range2334962/1
type: Range
persistent identity: range2334962
display id: range2334962
version: 1
direction: direction/forward
top level: BBa_K1170000
start: 1
end: 178
orientation: inline

TATA box
type: SequenceAnnotation
persistent identity: annotation2334963
display id: annotation2334963
version: 1
top level: BBa_K1170000
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1170000/annotation2334963/range2334963/1

https://synbiohub.org/public/igem/BBa_K1170000/annotation2334963/range2334963/1
type: Range
persistent identity: range2334963
display id: range2334963
version: 1
direction: direction/forward
top level: BBa_K1170000
start: 141
end: 147
orientation: inline

BBa_K116500
type: ComponentDefinition
persistent identity: BBa K116500
display id: BBa_K116500
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: OmpF promoter that is activated or repressesed by OmpR according to osmolarity.
creator: Chun-Ju Yang
created: 2008-10-28T12:00:00Z
modified: 2015-05-08T01:09:33Z
discontinued: false
dominant: false
group u list: _210_
m user id: 0
owner id: 2083
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Promoter OmpF is activated by phosphorylated OmpR at low osmolarity, and is repressesed by phosphorylated OmpR at high osmolarity.In Escherichia coli, osmoregulation is mediated in part by the actions of such a two-component system consisting of EnvZ and OmpR. These proteins act to control the relative levels of the outer membrane porin genes, ompF and ompC. At low osmo larity, OmpF predominates in the outer membrane, while at high osmolarity the OmpF porin is replaced by OmpC.OmpF has a larger pore and a faster ??ow rate than OmpC. Reference: K. Mattison, R. Oropeza, N. Byers, L. J. Kenney, J Mol Biol 315, 497 (Jan 25, 2002).
mutable notes: The osmolarity range of OmpF sensing still have to be confirmed
mutable provenance: Promoter OmpF is activated by phosphorylated OmpR at low osmolarity, and is repressesed by phosphorylated OmpR at high osmolarity.In Escherichia coli, osmoregulation is mediated in part by the actions of such a two-component system consisting of EnvZ and OmpR. These proteins act to control the relative levels of the outer membrane porin genes, ompF and ompC. At low osmo larity, OmpF predominates in the outer membrane, while at high osmolarity the OmpF porin is replaced by OmpC.OmpF has a larger pore and a faster ??ow rate than OmpC.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OmpR binding
sequence: https://synbiohub.org/public/igem/BBa_K116500_sequence/1

OmpR binding
type: SequenceAnnotation
persistent identity: annotation1994040
display id: annotation1994040
version: 1
top level: BBa_K116500
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K116500/annotation1994040/range1994040/1

https://synbiohub.org/public/igem/BBa_K116500/annotation1994040/range1994040/1
type: Range
persistent identity: range1994040
display id: range1994040
version: 1
direction: direction/forward
top level: BBa_K116500
start: 27
end: 88
orientation: inline

BBa_K165031
type: ComponentDefinition
persistent identity: BBa K165031
display id: BBa_K165031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mCYC promoter plus LexA binding sites
creator: Aaron Glieberman
created: 2008-10-28T12:00:00Z
modified: 2015-05-08T01:10:56Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2510
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a composite part containing the minimal promoter mCYC preceded by multiple consecutive binding sites for the LexA binding domain.
mutable notes: It was necessary to design primers for use in PCR to remove this part from its host plasmid and transfer it to a standard Biobrick vector
mutable provenance: Composite part was taken via PCR from a synthetic plasmid containing the sequence. The plasmid came from Lawrence Berkeley National Laboratory.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K165031_sequence/1

BBa_J64010
type: ComponentDefinition
persistent identity: BBa J64010
display id: BBa_J64010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lasI promoter
creator: Jeffrey J Tabor
created: 2007-01-15T12:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 88
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Promoter driving the lasI gene in Pseudomonas aeruginosa. Activated by LasR+3OC12HSL and not RhlR+C4HSL (Seed et al., 1995) ~4x stronger than the lasB promoter (BBa_R0079; Seed et al., 1995). Switch point ~0.1nM 3OC12HSL (Seed et al., 1995)
mutable notes: Promoter sequence cropped to 4nt upstream of Las box according to example of Whiteley and Greenberg, J. Bac, 2001
mutable provenance: Genomic sequence of Pseudomonas aeruginosa
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Las box
sequence annotation: +1
sequence: https://synbiohub.org/public/igem/BBa_J64010_sequence/1

Las box
type: SequenceAnnotation
persistent identity: annotation1910615
display id: annotation1910615
version: 1
top level: BBa_J64010
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_J64010/annotation1910615/range1910615/1

https://synbiohub.org/public/igem/BBa_J64010/annotation1910615/range1910615/1
type: Range
persistent identity: range1910615
display id: range1910615
version: 1
direction: direction/forward
top level: BBa_J64010
start: 5
end: 20
orientation: inline

+1
type: SequenceAnnotation
persistent identity: annotation1910616
display id: annotation1910616
version: 1
top level: BBa_J64010
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J64010/annotation1910616/range1910616/1

https://synbiohub.org/public/igem/BBa_J64010/annotation1910616/range1910616/1
type: Range
persistent identity: range1910616
display id: range1910616
version: 1
direction: direction/forward
top level: BBa_J64010
start: 53
end: 53
orientation: inline

BBa_J15502
type: ComponentDefinition
persistent identity: BBa J15502
display id: BBa_J15502
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: copA promoter
creator: Chris French
created: 2008-05-15T11:00:00Z
modified: 2015-08-31T04:08:32Z
discontinued: false
dominant: false
group u list: _163_
m user id: 0
owner id: 837
owning group id: 163
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Promoter of the copA copper efflux gene from Escherichia coli K12. This promoter is induced in the presence of copper ions, regulated by the activator protein CueR (CopR).
mutable notes: No special considerations
mutable provenance: Escherichia coli K12
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: -10
sequence annotation: CpxR
sequence: https://synbiohub.org/public/igem/BBa_J15502_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation1963239
display id: annotation1963239
version: 1
top level: BBa_J15502
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J15502/annotation1963239/range1963239/1

https://synbiohub.org/public/igem/BBa_J15502/annotation1963239/range1963239/1
type: Range
persistent identity: range1963239
display id: range1963239
version: 1
direction: direction/forward
top level: BBa_J15502
start: 248
end: 253
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1963240
display id: annotation1963240
version: 1
top level: BBa_J15502
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J15502/annotation1963240/range1963240/1

https://synbiohub.org/public/igem/BBa_J15502/annotation1963240/range1963240/1
type: Range
persistent identity: range1963240
display id: range1963240
version: 1
direction: direction/forward
top level: BBa_J15502
start: 273
end: 278
orientation: inline

CpxR
type: SequenceAnnotation
persistent identity: annotation1963241
display id: annotation1963241
version: 1
top level: BBa_J15502
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J15502/annotation1963241/range1963241/1

https://synbiohub.org/public/igem/BBa_J15502/annotation1963241/range1963241/1
type: Range
persistent identity: range1963241
display id: range1963241
version: 1
direction: direction/forward
top level: BBa_J15502
start: 214
end: 227
orientation: inline

pBAD
type: ComponentDefinition
persistent identity: BBa K206000
display id: BBa_K206000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBAD strong
creator: Amelia Hardjasa
created: 2009-10-13T11:00:00Z
modified: 2015-05-08T01:11:23Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _307_
m user id: 0
owner id: 4172
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: pBAD is an E.coli promoter that is induced by L-arabinose. K206000 is a mutagenized pBAD promoter that is responsive to lower concentrations of arabinose than wild type ( I13453 ) and, additionally, exhibits a higher maximum of protein expression as measured by coupling to a fluorescent reporter.
mutable notes: There were no special design considerations.
mutable provenance: The sequence was obtained by applying all mutations that upregulated AraC binding and subsequent promoter activity listed in reference [1].
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: promoter
sequence annotation: AraI2
sequence annotation: AraI1
sequence: https://synbiohub.org/public/igem/BBa_K206000_sequence/1

promoter
type: SequenceAnnotation
persistent identity: annotation2049252
display id: annotation2049252
version: 1
top level: pBAD
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K206000/annotation2049252/range2049252/1

https://synbiohub.org/public/igem/BBa_K206000/annotation2049252/range2049252/1
type: Range
persistent identity: range2049252
display id: range2049252
version: 1
direction: direction/forward
top level: pBAD
start: 1
end: 131
orientation: inline

AraI2
type: SequenceAnnotation
persistent identity: annotation2049254
display id: annotation2049254
version: 1
top level: pBAD
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K206000/annotation2049254/range2049254/1

https://synbiohub.org/public/igem/BBa_K206000/annotation2049254/range2049254/1
type: Range
persistent identity: range2049254
display id: range2049254
version: 1
direction: direction/forward
top level: pBAD
start: 61
end: 78
orientation: inline

AraI1
type: SequenceAnnotation
persistent identity: annotation2049253
display id: annotation2049253
version: 1
top level: pBAD
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K206000/annotation2049253/range2049253/1

https://synbiohub.org/public/igem/BBa_K206000/annotation2049253/range2049253/1
type: Range
persistent identity: range2049253
display id: range2049253
version: 1
direction: direction/forward
top level: pBAD
start: 40
end: 57
orientation: inline

BBa_K2110007
type: ComponentDefinition
persistent identity: BBa K2110007
display id: BBa_K2110007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TPA transporting protein gene expression system
creator: Le He
created: 2016-10-05T11:00:00Z
modified: 2016-10-06T01:26:31Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2578_
m user id: 32957
owner id: 32957
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part consists of the enhanced promoter PGK1(BBa_K2110012), the tpaK gene(BBa_K2110003), and the terminator CYC1（BBa_K2110000）. The tpaK gene encodes the terephthalic acid (TPA) transporting protein. TPA is not a kind of nutrient substance for most organisms, including common chassis organisms like E.coli and Saccharomyces cerevisiae. However, as a kind of TPAdegradable organism, RhodococcusjostiiRHA1 can transport TPA into their cells because of the TPA transporting protein gene tpaK in their genome. This gene is also verified to be useful in Saccharomyces cerevisiae.
mutable notes: /
mutable provenance: BBa_K2110012 BBa_K2110003 BBa_K2110000
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K2110012
component: BBa_K2110000
component: BBa_K2110003
sequence annotation: BBa_K2110000
sequence annotation: BBa_K2110012
sequence annotation: BBa_K2110003
sequence: https://synbiohub.org/public/igem/BBa_K2110007_sequence/1

BBa_K2110012
type: Component
persistent identity: component2486545
display id: component2486545
version: 1
top level: BBa_K2110007
access: public
definition: https://synbiohub.org/public/igem/BBa_K2110012/1

BBa_K2110000
type: Component
persistent identity: component2486548
display id: component2486548
version: 1
top level: BBa_K2110007
access: public
definition: https://synbiohub.org/public/igem/BBa_K2110000/1

BBa_K2110003
type: Component
persistent identity: component2486547
display id: component2486547
version: 1
top level: BBa_K2110007
access: public
definition: https://synbiohub.org/public/igem/BBa_K2110003/1

BBa_K2110000
type: SequenceAnnotation
persistent identity: annotation2486548
display id: annotation2486548
version: 1
top level: BBa_K2110007
location: https://synbiohub.org/public/igem/BBa_K2110007/annotation2486548/range2486548/1
component: BBa_K2110000

https://synbiohub.org/public/igem/BBa_K2110007/annotation2486548/range2486548/1
type: Range
persistent identity: range2486548
display id: range2486548
version: 1
direction: direction/reverse
top level: BBa_K2110007
start: 2178
end: 2425
orientation: inline

BBa_K2110012
type: SequenceAnnotation
persistent identity: annotation2486545
display id: annotation2486545
version: 1
top level: BBa_K2110007
location: https://synbiohub.org/public/igem/BBa_K2110007/annotation2486545/range2486545/1
component: BBa_K2110012

https://synbiohub.org/public/igem/BBa_K2110007/annotation2486545/range2486545/1
type: Range
persistent identity: range2486545
display id: range2486545
version: 1
direction: direction/reverse
top level: BBa_K2110007
start: 1
end: 781
orientation: inline

BBa_K2110003
type: SequenceAnnotation
persistent identity: annotation2486547
display id: annotation2486547
version: 1
top level: BBa_K2110007
location: https://synbiohub.org/public/igem/BBa_K2110007/annotation2486547/range2486547/1
component: BBa_K2110003

https://synbiohub.org/public/igem/BBa_K2110007/annotation2486547/range2486547/1
type: Range
persistent identity: range2486547
display id: range2486547
version: 1
direction: direction/reverse
top level: BBa_K2110007
start: 790
end: 2169
orientation: inline

BBa_K266000
type: ComponentDefinition
persistent identity: BBa K266000
display id: BBa_K266000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PAI+LasR -> LuxI (AI)
creator: UNAM FCIENCIAS-CINVESTAV IRA
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _363_
m user id: 0
owner id: 1955
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This biobrick has a promoter inducible by PAI+LasR (BBa_R0079)and produces LuxI enzyme (BBa_F1610). This enzyme produces 3OC6HSL (AI).
mutable notes: None
mutable provenance: The DNA of BBa_R0079 and BBa_F1610 biobricks where used.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_F1610
component: BBa_R0079
sequence annotation: BBa_R0079
sequence annotation: BBa_F1610
sequence: https://synbiohub.org/public/igem/BBa_K266000_sequence/1

BBa_F1610
type: Component
persistent identity: component2256062
display id: component2256062
version: 1
top level: BBa_K266000
access: public
definition: https://synbiohub.org/public/igem/BBa_F1610/1

BBa_R0079
type: Component
persistent identity: component2256049
display id: component2256049
version: 1
top level: BBa_K266000
access: public
definition: LasR+PAI

BBa_R0079
type: SequenceAnnotation
persistent identity: annotation2256049
display id: annotation2256049
version: 1
top level: BBa_K266000
location: https://synbiohub.org/public/igem/BBa_K266000/annotation2256049/range2256049/1
component: BBa_R0079

https://synbiohub.org/public/igem/BBa_K266000/annotation2256049/range2256049/1
type: Range
persistent identity: range2256049
display id: range2256049
version: 1
direction: direction/reverse
top level: BBa_K266000
start: 1
end: 157
orientation: inline

BBa_F1610
type: SequenceAnnotation
persistent identity: annotation2256062
display id: annotation2256062
version: 1
top level: BBa_K266000
location: https://synbiohub.org/public/igem/BBa_K266000/annotation2256062/range2256062/1
component: BBa_F1610

https://synbiohub.org/public/igem/BBa_K266000/annotation2256062/range2256062/1
type: Range
persistent identity: range2256062
display id: range2256062
version: 1
direction: direction/reverse
top level: BBa_K266000
start: 166
end: 963
orientation: inline

Pvir
type: ComponentDefinition
persistent identity: BBa K389003
display id: BBa_K389003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: virB promoter
creator: Jonas Aretz
created: 2010-08-09T11:00:00Z
modified: 2015-05-08T01:12:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a ''vir'' promotor from ''Agrobacterium tumefaciens'' C58. It is located on the TI-plasmid before the ''virB'' genes. It is activated by phosphorylated ''virG'' response regulator.
mutable notes: contains already a RBS
mutable provenance: ''A. tumefaciens'' C58, TI-plasmid
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: rbs
sequence annotation: -35
sequence annotation: vir-box
sequence: https://synbiohub.org/public/igem/BBa_K389003_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation2077851
display id: annotation2077851
version: 1
top level: Pvir
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K389003/annotation2077851/range2077851/1

https://synbiohub.org/public/igem/BBa_K389003/annotation2077851/range2077851/1
type: Range
persistent identity: range2077851
display id: range2077851
version: 1
direction: direction/forward
top level: Pvir
start: 113
end: 117
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2077849
display id: annotation2077849
version: 1
top level: Pvir
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K389003/annotation2077849/range2077849/1

https://synbiohub.org/public/igem/BBa_K389003/annotation2077849/range2077849/1
type: Range
persistent identity: range2077849
display id: range2077849
version: 1
direction: direction/forward
top level: Pvir
start: 153
end: 157
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2077850
display id: annotation2077850
version: 1
top level: Pvir
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K389003/annotation2077850/range2077850/1

https://synbiohub.org/public/igem/BBa_K389003/annotation2077850/range2077850/1
type: Range
persistent identity: range2077850
display id: range2077850
version: 1
direction: direction/forward
top level: Pvir
start: 89
end: 93
orientation: inline

vir-box
type: SequenceAnnotation
persistent identity: annotation2077848
display id: annotation2077848
version: 1
top level: Pvir
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K389003/annotation2077848/range2077848/1

https://synbiohub.org/public/igem/BBa_K389003/annotation2077848/range2077848/1
type: Range
persistent identity: range2077848
display id: range2077848
version: 1
direction: direction/forward
top level: Pvir
start: 33
end: 44
orientation: inline

BBa_K118011
type: ComponentDefinition
persistent identity: BBa K118011
display id: BBa_K118011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PcstA (glucose-repressible promoter)
creator: Andrew Hall
created: 2008-08-18T11:00:00Z
modified: 2015-05-08T01:09:36Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is the promoter for the ''Eschaerichia coli'' JM109 ''cstA'' gene. It includes the CRP-binding site and the RNA polymerase-binding site. Low glucose concentration results in increased activity by adenylate cyclase. cAMP binds to the cAMP receptor protein, which, in its bound form, is able to associate with the promoter and promote transcription of the downstream gene. (''cstA'' encodes the carbon starvation protein.)
mutable notes: No special considerations
mutable provenance: ''Eschaerichia coli'' JM109 genomic DNA
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: cAMP receptor protein binding site
sequence annotation: RNA polymerase binding site
sequence: https://synbiohub.org/public/igem/BBa_K118011_sequence/1

cAMP receptor protein binding site
type: SequenceAnnotation
persistent identity: annotation1972435
display id: annotation1972435
version: 1
top level: BBa_K118011
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K118011/annotation1972435/range1972435/1

https://synbiohub.org/public/igem/BBa_K118011/annotation1972435/range1972435/1
type: Range
persistent identity: range1972435
display id: range1972435
version: 1
direction: direction/forward
top level: BBa_K118011
start: 1
end: 42
orientation: inline

RNA polymerase binding site
type: SequenceAnnotation
persistent identity: annotation1972436
display id: annotation1972436
version: 1
top level: BBa_K118011
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K118011/annotation1972436/range1972436/1

https://synbiohub.org/public/igem/BBa_K118011/annotation1972436/range1972436/1
type: Range
persistent identity: range1972436
display id: range1972436
version: 1
direction: direction/forward
top level: BBa_K118011
start: 101
end: 131
orientation: inline

BBa_I721001
type: ComponentDefinition
persistent identity: BBa I721001
display id: BBa_I721001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lead Promoter
creator: Jeffrey Hofmann
created: 2007-10-25T11:00:00Z
modified: 2015-08-31T04:07:53Z
discontinued: false
dominant: false
experience: experience/None
group u list: _113_
m user id: 0
owner id: 1531
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Lead Promoter (Part 4) messed up EcoRI site
mutable notes: none
mutable provenance: ralstonia metallidurans
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I721001_sequence/1

BBa_K1315001
type: ComponentDefinition
persistent identity: BBa K1315001
display id: BBa_K1315001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: The pqsA promoter from Pseudomonas
creator: Dimitrios Michailidis
created: 2014-09-19T11:00:00Z
modified: 2015-05-08T01:09:48Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1690_
m user id: 0
owner id: 21812
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: The Pseudomonas quinolone signal (PQS) is imperative in modulating mechanisms that enable Pseudomonas aeruginosa to survive at times of stress. P. aeruginosa is the main pathogen responsible for severe deterioration in the Cystic Fibrosis lungs. The production of this signal relies on the pqsABCDE operon. The transcriptional factor PqsR (BBa_K1315000) positively regulates this operon. http://www.ncbi.nlm.nih.gov/pubmed/25225275 http://www.ncbi.nlm.nih.gov/pubmed/16735731 http://www.ncbi.nlm.nih.gov/pubmed/21539583 http://www.ncbi.nlm.nih.gov/pubmed/?term=pqsr-dependent+and+pqsr-independent+regulation+of+motility+and+biofilm+formation+by+pqs http://www.ncbi.nlm.nih.gov/pubmed/12393198
mutable notes: The sequence did not have any restriction sites to remove so it was immediately PCRed out of the genome and had the registry prefix and suffix added to it. It was then ligated into the pSB1C3 vector.
mutable provenance: The source of this part is the genome of Pseudomonas aeruginosa PAO1 provided by the Robert Ryan lab in the Molecular Microbiology department of the University of Dundee, UK.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: pqsABCDE
sequence: https://synbiohub.org/public/igem/BBa_K1315001_sequence/1

pqsABCDE
type: SequenceAnnotation
persistent identity: annotation2384758
display id: annotation2384758
version: 1
top level: BBa_K1315001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1315001/annotation2384758/range2384758/1

https://synbiohub.org/public/igem/BBa_K1315001/annotation2384758/range2384758/1
type: Range
persistent identity: range2384758
display id: range2384758
version: 1
direction: direction/forward
top level: BBa_K1315001
start: 1
end: 92
orientation: inline

BBa_K180014
type: ComponentDefinition
persistent identity: BBa K180014
display id: BBa_K180014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Rock-paper-scissors - Paper primary plasmid
creator: Richard Wilson, Stephanie Chambers
created: 2009-10-20T11:00:00Z
modified: 2015-05-08T01:11:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _278_
m user id: 0
owner id: 4082
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: Composite part, consisting of BBa_K180005 and BBa_K180015, but with BBa_K180005 reading along the reverse strand, and BBa_K180015 reading along the forward strand. Forms the 'paper' primary plasmid in Team:Southampton's rock-paper-scissors project design.
mutable notes: BBa_K180005 reading along reverse strand, and BBa_K180015 reading along the forward strand to ensure the constitutive promoter BBa_J23119 (present in BBa_K180005) has no effect over GFP or RhlI expression in BBa_K180015.
mutable provenance: See relevant biobrick component parts (BBa_K180011 and BBa_K180012).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: luxR
sequence annotation: B0034
sequence annotation: Ptac
sequence annotation: E0040
sequence annotation: C0062
sequence annotation: conserved
sequence annotation: K180015
sequence annotation: rhlI
sequence annotation: GFPmut3b
sequence annotation: B0015
sequence annotation: -10
sequence annotation: K180005
sequence annotation: lacIq
sequence annotation: C0170
sequence annotation: K180000
sequence annotation: -35
sequence annotation: J23119
sequence: https://synbiohub.org/public/igem/BBa_K180014_sequence/1

luxR
type: SequenceAnnotation
persistent identity: annotation2058326
display id: annotation2058326
version: 1
top level: BBa_K180014
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058326/range2058326/1

https://synbiohub.org/public/igem/BBa_K180014/annotation2058326/range2058326/1
type: Range
persistent identity: range2058326
display id: range2058326
version: 1
direction: direction/reverse
top level: BBa_K180014
start: 169
end: 918
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2058327
display id: annotation2058327
version: 1
top level: BBa_K180014
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058327/range2058327/1

https://synbiohub.org/public/igem/BBa_K180014/annotation2058327/range2058327/1
type: Range
persistent identity: range2058327
display id: range2058327
version: 1
direction: direction/forward
top level: BBa_K180014
start: 2443
end: 2454
orientation: inline

Ptac
type: SequenceAnnotation
persistent identity: annotation2058338
display id: annotation2058338
version: 1
top level: BBa_K180014
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058338/range2058338/1

https://synbiohub.org/public/igem/BBa_K180014/annotation2058338/range2058338/1
type: Range
persistent identity: range2058338
display id: range2058338
version: 1
direction: direction/forward
top level: BBa_K180014
start: 2312
end: 2339
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2058329
display id: annotation2058329
version: 1
top level: BBa_K180014
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058329/range2058329/1

https://synbiohub.org/public/igem/BBa_K180014/annotation2058329/range2058329/1
type: Range
persistent identity: range2058329
display id: range2058329
version: 1
direction: direction/forward
top level: BBa_K180014
start: 3189
end: 3200
orientation: inline

E0040
type: SequenceAnnotation
persistent identity: annotation2058339
display id: annotation2058339
version: 1
top level: BBa_K180014
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058339/range2058339/1

https://synbiohub.org/public/igem/BBa_K180014/annotation2058339/range2058339/1
type: Range
persistent identity: range2058339
display id: range2058339
version: 1
direction: direction/forward
top level: BBa_K180014
start: 2461
end: 3180
orientation: inline

C0062
type: SequenceAnnotation
persistent identity: annotation2058325
display id: annotation2058325
version: 1
top level: BBa_K180014
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058325/range2058325/1

https://synbiohub.org/public/igem/BBa_K180014/annotation2058325/range2058325/1
type: Range
persistent identity: range2058325
display id: range2058325
version: 1
direction: direction/reverse
top level: BBa_K180014
start: 163
end: 918
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2058332
display id: annotation2058332
version: 1
top level: BBa_K180014
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058332/range2058332/1

https://synbiohub.org/public/igem/BBa_K180014/annotation2058332/range2058332/1
type: Range
persistent identity: range2058332
display id: range2058332
version: 1
direction: direction/reverse
top level: BBa_K180014
start: 929
end: 932
orientation: inline

K180015
type: SequenceAnnotation
persistent identity: annotation2058321
display id: annotation2058321
version: 1
top level: BBa_K180014
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058321/range2058321/1

https://synbiohub.org/public/igem/BBa_K180014/annotation2058321/range2058321/1
type: Range
persistent identity: range2058321
display id: range2058321
version: 1
direction: direction/forward
top level: BBa_K180014
start: 988
end: 3952
orientation: inline

rhlI
type: SequenceAnnotation
persistent identity: annotation2058342
display id: annotation2058342
version: 1
top level: BBa_K180014
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058342/range2058342/1

https://synbiohub.org/public/igem/BBa_K180014/annotation2058342/range2058342/1
type: Range
persistent identity: range2058342
display id: range2058342
version: 1
direction: direction/forward
top level: BBa_K180014
start: 3207
end: 3815
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2058328
display id: annotation2058328
version: 1
top level: BBa_K180014
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058328/range2058328/1

https://synbiohub.org/public/igem/BBa_K180014/annotation2058328/range2058328/1
type: Range
persistent identity: range2058328
display id: range2058328
version: 1
direction: direction/forward
top level: BBa_K180014
start: 2447
end: 2450
orientation: inline

GFPmut3b
type: SequenceAnnotation
persistent identity: annotation2058340
display id: annotation2058340
version: 1
top level: BBa_K180014
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058340/range2058340/1

https://synbiohub.org/public/igem/BBa_K180014/annotation2058340/range2058340/1
type: Range
persistent identity: range2058340
display id: range2058340
version: 1
direction: direction/forward
top level: BBa_K180014
start: 2461
end: 3180
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2058343
display id: annotation2058343
version: 1
top level: BBa_K180014
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058343/range2058343/1

https://synbiohub.org/public/igem/BBa_K180014/annotation2058343/range2058343/1
type: Range
persistent identity: range2058343
display id: range2058343
version: 1
direction: direction/forward
top level: BBa_K180014
start: 3824
end: 3952
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2058330
display id: annotation2058330
version: 1
top level: BBa_K180014
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058330/range2058330/1

https://synbiohub.org/public/igem/BBa_K180014/annotation2058330/range2058330/1
type: Range
persistent identity: range2058330
display id: range2058330
version: 1
direction: direction/forward
top level: BBa_K180014
start: 3193
end: 3196
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2058331
display id: annotation2058331
version: 1
top level: BBa_K180014
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058331/range2058331/1

https://synbiohub.org/public/igem/BBa_K180014/annotation2058331/range2058331/1
type: Range
persistent identity: range2058331
display id: range2058331
version: 1
direction: direction/reverse
top level: BBa_K180014
start: 925
end: 936
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2058335
display id: annotation2058335
version: 1
top level: BBa_K180014
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058335/range2058335/1

https://synbiohub.org/public/igem/BBa_K180014/annotation2058335/range2058335/1
type: Range
persistent identity: range2058335
display id: range2058335
version: 1
direction: direction/reverse
top level: BBa_K180014
start: 951
end: 956
orientation: inline

K180005
type: SequenceAnnotation
persistent identity: annotation2058320
display id: annotation2058320
version: 1
top level: BBa_K180014
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058320/range2058320/1

https://synbiohub.org/public/igem/BBa_K180014/annotation2058320/range2058320/1
type: Range
persistent identity: range2058320
display id: range2058320
version: 1
direction: direction/reverse
top level: BBa_K180014
start: 1
end: 979
orientation: inline

lacIq
type: SequenceAnnotation
persistent identity: annotation2058337
display id: annotation2058337
version: 1
top level: BBa_K180014
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058337/range2058337/1

https://synbiohub.org/public/igem/BBa_K180014/annotation2058337/range2058337/1
type: Range
persistent identity: range2058337
display id: range2058337
version: 1
direction: direction/forward
top level: BBa_K180014
start: 988
end: 2069
orientation: inline

C0170
type: SequenceAnnotation
persistent identity: annotation2058341
display id: annotation2058341
version: 1
top level: BBa_K180014
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058341/range2058341/1

https://synbiohub.org/public/igem/BBa_K180014/annotation2058341/range2058341/1
type: Range
persistent identity: range2058341
display id: range2058341
version: 1
direction: direction/forward
top level: BBa_K180014
start: 3207
end: 3815
orientation: inline

K180000
type: SequenceAnnotation
persistent identity: annotation2058336
display id: annotation2058336
version: 1
top level: BBa_K180014
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058336/range2058336/1

https://synbiohub.org/public/igem/BBa_K180014/annotation2058336/range2058336/1
type: Range
persistent identity: range2058336
display id: range2058336
version: 1
direction: direction/forward
top level: BBa_K180014
start: 988
end: 2434
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2058334
display id: annotation2058334
version: 1
top level: BBa_K180014
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058334/range2058334/1

https://synbiohub.org/public/igem/BBa_K180014/annotation2058334/range2058334/1
type: Range
persistent identity: range2058334
display id: range2058334
version: 1
direction: direction/reverse
top level: BBa_K180014
start: 975
end: 979
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2058324
display id: annotation2058324
version: 1
top level: BBa_K180014
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058324/range2058324/1

https://synbiohub.org/public/igem/BBa_K180014/annotation2058324/range2058324/1
type: Range
persistent identity: range2058324
display id: range2058324
version: 1
direction: direction/reverse
top level: BBa_K180014
start: 1
end: 129
orientation: inline

J23119
type: SequenceAnnotation
persistent identity: annotation2058333
display id: annotation2058333
version: 1
top level: BBa_K180014
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058333/range2058333/1

https://synbiohub.org/public/igem/BBa_K180014/annotation2058333/range2058333/1
type: Range
persistent identity: range2058333
display id: range2058333
version: 1
direction: direction/reverse
top level: BBa_K180014
start: 945
end: 979
orientation: inline

BBa_K266005
type: ComponentDefinition
persistent identity: BBa K266005
display id: BBa_K266005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PAI+LasR -> LasI & AI+LuxR --| LasI
creator: UNAM FCIENCIAS-CINVESTAV IRA
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _363_
m user id: 0
owner id: 1955
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: BBa_K091146 promoter (PAI+LasR inducible & AI+LuxR repressible) controls the expresion of LasI enzyme, no terminator
mutable notes: None
mutable provenance: The biobricks BBa_K091146 and BBa_S03154 where used to build this biobrick
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_K091146
component: BBa_S03154
sequence annotation: BBa_K091146
sequence annotation: BBa_S03154
sequence: https://synbiohub.org/public/igem/BBa_K266005_sequence/1

BBa_K091146
type: Component
persistent identity: component2246677
display id: component2246677
version: 1
top level: BBa_K266005
access: public
definition: BBa_K091146

BBa_S03154
type: Component
persistent identity: component2246684
display id: component2246684
version: 1
top level: BBa_K266005
access: public
definition: https://synbiohub.org/public/igem/BBa_S03154/1

BBa_K091146
type: SequenceAnnotation
persistent identity: annotation2246677
display id: annotation2246677
version: 1
top level: BBa_K266005
location: https://synbiohub.org/public/igem/BBa_K266005/annotation2246677/range2246677/1
component: BBa_K091146

https://synbiohub.org/public/igem/BBa_K266005/annotation2246677/range2246677/1
type: Range
persistent identity: range2246677
display id: range2246677
version: 1
direction: direction/reverse
top level: BBa_K266005
start: 1
end: 126
orientation: inline

BBa_S03154
type: SequenceAnnotation
persistent identity: annotation2246684
display id: annotation2246684
version: 1
top level: BBa_K266005
location: https://synbiohub.org/public/igem/BBa_K266005/annotation2246684/range2246684/1
component: BBa_S03154

https://synbiohub.org/public/igem/BBa_K266005/annotation2246684/range2246684/1
type: Range
persistent identity: range2246684
display id: range2246684
version: 1
direction: direction/reverse
top level: BBa_K266005
start: 135
end: 819
orientation: inline

BBa_K165001
type: ComponentDefinition
persistent identity: BBa K165001
display id: BBa_K165001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pGAL1+ w/XhoI sites
creator: John Szymanski
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is the GAL1 inducible promoter for use in Saccharomyces cerevisiae. It is repressed in the presence of glucose and induced in the presence of galactose. Raffinose is a neutral sugar source the neither induces nor represses the promoter. pGAL1 normally has an "all-or-nothing" response to galactose, but Christina Smolke has shown that knockout of the Gal2 gene gives pGAL1 a tunable, linear response to galactose.
mutable notes: none
mutable provenance: From the Saccharomyces cerevisiae genome, via Caroline Ajo-Franklin.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K165001_sequence/1

BBa_I746365
type: ComponentDefinition
persistent identity: BBa I746365
display id: BBa_I746365
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PLL promoter from P4 phage
creator: Stefan Milde
created: 2007-09-10T11:00:00Z
modified: 2015-08-31T04:08:04Z
discontinued: false
dominant: false
group u list: _116_
m user id: 0
owner id: 2122
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the PLL promoter taken from the P4 phage genome. It is an inducible promoter that is activated by a class of activators, including P2 ogr (I746350), PSP3 pag (I746351) and phiR73 delta (I746352). These different activators should cause different levels of activity of the PLL promoter.
mutable notes: no special considerations
mutable provenance: P4 phage genome sequence
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: PLL
sequence: https://synbiohub.org/public/igem/BBa_I746365_sequence/1

PLL
type: SequenceAnnotation
persistent identity: annotation1943788
display id: annotation1943788
version: 1
top level: BBa_I746365
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I746365/annotation1943788/range1943788/1

https://synbiohub.org/public/igem/BBa_I746365/annotation1943788/range1943788/1
type: Range
persistent identity: range1943788
display id: range1943788
version: 1
direction: direction/forward
top level: BBa_I746365
start: 1
end: 92
orientation: inline

pBAD:SDnaK
type: ComponentDefinition
persistent identity: BBa K538302
display id: BBa_K538302
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBAD + RBS + SheDnaK
creator: Bas Stringer
created: 2011-09-07T11:00:00Z
modified: 2015-05-08T01:12:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _704_
m user id: 0
owner id: 9985
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_K538002
component: BBa_K206000
sequence annotation: BBa_K538002
sequence annotation: BBa_B0034
sequence annotation: BBa_K206000
sequence: https://synbiohub.org/public/igem/BBa_K538302_sequence/1

BBa_B0034
type: Component
persistent identity: component2126779
display id: component2126779
version: 1
top level: pBAD:SDnaK
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K538002
type: Component
persistent identity: component2126782
display id: component2126782
version: 1
top level: pBAD:SDnaK
access: public
definition: https://synbiohub.org/public/igem/BBa_K538002/1

BBa_K206000
type: Component
persistent identity: component2126777
display id: component2126777
version: 1
top level: pBAD:SDnaK
access: public
definition: pBAD

BBa_K538002
type: SequenceAnnotation
persistent identity: annotation2126782
display id: annotation2126782
version: 1
top level: pBAD:SDnaK
location: https://synbiohub.org/public/igem/BBa_K538302/annotation2126782/range2126782/1
component: BBa_K538002

https://synbiohub.org/public/igem/BBa_K538302/annotation2126782/range2126782/1
type: Range
persistent identity: range2126782
display id: range2126782
version: 1
direction: direction/reverse
top level: pBAD:SDnaK
start: 157
end: 2076
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2126779
display id: annotation2126779
version: 1
top level: pBAD:SDnaK
location: https://synbiohub.org/public/igem/BBa_K538302/annotation2126779/range2126779/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K538302/annotation2126779/range2126779/1
type: Range
persistent identity: range2126779
display id: range2126779
version: 1
direction: direction/reverse
top level: pBAD:SDnaK
start: 139
end: 150
orientation: inline

BBa_K206000
type: SequenceAnnotation
persistent identity: annotation2126777
display id: annotation2126777
version: 1
top level: pBAD:SDnaK
location: https://synbiohub.org/public/igem/BBa_K538302/annotation2126777/range2126777/1
component: BBa_K206000

https://synbiohub.org/public/igem/BBa_K538302/annotation2126777/range2126777/1
type: Range
persistent identity: range2126777
display id: range2126777
version: 1
direction: direction/reverse
top level: pBAD:SDnaK
start: 1
end: 130
orientation: inline

BBa_J64001
type: ComponentDefinition
persistent identity: BBa J64001
display id: BBa_J64001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: psicA from Salmonella
creator: Dan Widmaier
created: 2008-01-14T12:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _98_
m user id: 0
owner id: 2407
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The sicA promoter is a regulatory element in the Salmonella SPI-1 secretion system. It controls the expression of secreted effector proteins in the natural system and turns on late. Please reference Temme et al, 2008, JMB for exact dynamics of this promoter and associated feedback mechanisms. This promoter only functions in Salmonella strains containing SPI-1.
mutable notes: This part can only be used in salmonella strains.
mutable provenance: Salmonella LT2 Genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35 Hexamer
sequence annotation: +1 Transcriptional Start Site
sequence annotation: sicA Promoter
sequence annotation: -10 Hexamer
sequence annotation: InvF:SicA Binding Box
sequence: https://synbiohub.org/public/igem/BBa_J64001_sequence/1

-35 Hexamer
type: SequenceAnnotation
persistent identity: annotation1959629
display id: annotation1959629
version: 1
top level: BBa_J64001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J64001/annotation1959629/range1959629/1

https://synbiohub.org/public/igem/BBa_J64001/annotation1959629/range1959629/1
type: Range
persistent identity: range1959629
display id: range1959629
version: 1
direction: direction/forward
top level: BBa_J64001
start: 71
end: 77
orientation: inline

+1 Transcriptional Start Site
type: SequenceAnnotation
persistent identity: annotation1959631
display id: annotation1959631
version: 1
top level: BBa_J64001
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J64001/annotation1959631/range1959631/1

https://synbiohub.org/public/igem/BBa_J64001/annotation1959631/range1959631/1
type: Range
persistent identity: range1959631
display id: range1959631
version: 1
direction: direction/forward
top level: BBa_J64001
start: 106
end: 106
orientation: inline

sicA Promoter
type: SequenceAnnotation
persistent identity: annotation1959627
display id: annotation1959627
version: 1
top level: BBa_J64001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J64001/annotation1959627/range1959627/1

https://synbiohub.org/public/igem/BBa_J64001/annotation1959627/range1959627/1
type: Range
persistent identity: range1959627
display id: range1959627
version: 1
direction: direction/forward
top level: BBa_J64001
start: 1
end: 143
orientation: inline

-10 Hexamer
type: SequenceAnnotation
persistent identity: annotation1959630
display id: annotation1959630
version: 1
top level: BBa_J64001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J64001/annotation1959630/range1959630/1

https://synbiohub.org/public/igem/BBa_J64001/annotation1959630/range1959630/1
type: Range
persistent identity: range1959630
display id: range1959630
version: 1
direction: direction/forward
top level: BBa_J64001
start: 94
end: 100
orientation: inline

InvF:SicA Binding Box
type: SequenceAnnotation
persistent identity: annotation1959628
display id: annotation1959628
version: 1
top level: BBa_J64001
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_J64001/annotation1959628/range1959628/1

https://synbiohub.org/public/igem/BBa_J64001/annotation1959628/range1959628/1
type: Range
persistent identity: range1959628
display id: range1959628
version: 1
direction: direction/forward
top level: BBa_J64001
start: 54
end: 65
orientation: inline

BBa_K106699
type: ComponentDefinition
persistent identity: BBa K106699
display id: BBa_K106699
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Gal1 Promoter
creator: Andrew Horwitz
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:09:00Z
discontinued: false
dominant: false
group u list: _226_
m user id: 0
owner id: 2659
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Galactose-inducible promoter (strong)!
mutable notes: None.
mutable provenance: Yeast genomic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K106699_sequence/1

BBa_J61051
type: ComponentDefinition
persistent identity: BBa J61051
display id: BBa_J61051
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [Psal1]
creator: John Anderson
created: 2007-02-20T12:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Salicylate promoter and nahR
mutable notes: nonh
mutable provenance: non
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J61051_sequence/1

BBa_K180012
type: ComponentDefinition
persistent identity: BBa K180012
display id: BBa_K180012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Rock - Primary plasmid (part 2) [tac pR, mCherry and LasI generator]
creator: Richard Wilson, Stephanie Chambers
created: 2009-10-18T11:00:00Z
modified: 2015-05-08T01:11:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _278_
m user id: 0
owner id: 4082
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Part of the iGEM2009 Team:Southampton rock-paper-scissors 'rock' primary plasmid.
mutable notes: Regulated expression of LasI (C0178) and mCherry (K180008) by tac pR (K180000), controlled by presence of lac/IPTG or trp in the cell.
mutable provenance: See relevant basic biobrick parts (K180000, B0034, K180008, B0015)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0010
component: BBa_K180008
component: BBa_B0034
component: BBa_K180000
component: BBa_B0012
component: BBa_C0178
sequence annotation: BBa_B0034
sequence annotation: BBa_C0178
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_K180000
sequence annotation: BBa_K180008
sequence: https://synbiohub.org/public/igem/BBa_K180012_sequence/1

BBa_B0010
type: Component
persistent identity: component2053933
display id: component2053933
version: 1
top level: BBa_K180012
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_K180008
type: Component
persistent identity: component2053928
display id: component2053928
version: 1
top level: BBa_K180012
access: public
definition: https://synbiohub.org/public/igem/BBa_K180008/1

BBa_B0034
type: Component
persistent identity: component2053930
display id: component2053930
version: 1
top level: BBa_K180012
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: Component
persistent identity: component2053926
display id: component2053926
version: 1
top level: BBa_K180012
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K180000
type: Component
persistent identity: component2053924
display id: component2053924
version: 1
top level: BBa_K180012
access: public
definition: BBa_K180000

BBa_B0012
type: Component
persistent identity: component2053935
display id: component2053935
version: 1
top level: BBa_K180012
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0178
type: Component
persistent identity: component2053932
display id: component2053932
version: 1
top level: BBa_K180012
access: public
definition: https://synbiohub.org/public/igem/BBa_C0178/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2053930
display id: annotation2053930
version: 1
top level: BBa_K180012
location: https://synbiohub.org/public/igem/BBa_K180012/annotation2053930/range2053930/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K180012/annotation2053930/range2053930/1
type: Range
persistent identity: range2053930
display id: range2053930
version: 1
direction: direction/reverse
top level: BBa_K180012
start: 2190
end: 2201
orientation: inline

BBa_C0178
type: SequenceAnnotation
persistent identity: annotation2053932
display id: annotation2053932
version: 1
top level: BBa_K180012
location: https://synbiohub.org/public/igem/BBa_K180012/annotation2053932/range2053932/1
component: BBa_C0178

https://synbiohub.org/public/igem/BBa_K180012/annotation2053932/range2053932/1
type: Range
persistent identity: range2053932
display id: range2053932
version: 1
direction: direction/reverse
top level: BBa_K180012
start: 2208
end: 2816
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2053935
display id: annotation2053935
version: 1
top level: BBa_K180012
location: https://synbiohub.org/public/igem/BBa_K180012/annotation2053935/range2053935/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K180012/annotation2053935/range2053935/1
type: Range
persistent identity: range2053935
display id: range2053935
version: 1
direction: direction/forward
top level: BBa_K180012
start: 2913
end: 2953
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2053933
display id: annotation2053933
version: 1
top level: BBa_K180012
location: https://synbiohub.org/public/igem/BBa_K180012/annotation2053933/range2053933/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K180012/annotation2053933/range2053933/1
type: Range
persistent identity: range2053933
display id: range2053933
version: 1
direction: direction/forward
top level: BBa_K180012
start: 2825
end: 2904
orientation: inline

BBa_K180000
type: SequenceAnnotation
persistent identity: annotation2053924
display id: annotation2053924
version: 1
top level: BBa_K180012
location: https://synbiohub.org/public/igem/BBa_K180012/annotation2053924/range2053924/1
component: BBa_K180000

https://synbiohub.org/public/igem/BBa_K180012/annotation2053924/range2053924/1
type: Range
persistent identity: range2053924
display id: range2053924
version: 1
direction: direction/reverse
top level: BBa_K180012
start: 1
end: 1447
orientation: inline

BBa_K180008
type: SequenceAnnotation
persistent identity: annotation2053928
display id: annotation2053928
version: 1
top level: BBa_K180012
location: https://synbiohub.org/public/igem/BBa_K180012/annotation2053928/range2053928/1
component: BBa_K180008

https://synbiohub.org/public/igem/BBa_K180012/annotation2053928/range2053928/1
type: Range
persistent identity: range2053928
display id: range2053928
version: 1
direction: direction/reverse
top level: BBa_K180012
start: 1474
end: 2181
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2053926
display id: annotation2053926
version: 1
top level: BBa_K180012
location: https://synbiohub.org/public/igem/BBa_K180012/annotation2053926/range2053926/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K180012/annotation2053926/range2053926/1
type: Range
persistent identity: range2053926
display id: range2053926
version: 1
direction: direction/reverse
top level: BBa_K180012
start: 1456
end: 1467
orientation: inline

RhI
type: ComponentDefinition
persistent identity: BBa I14017
display id: BBa_I14017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: P(Rhl)
creator: Vikram Vijayan, Allen Hsu, Lawrence Fomundam
created: 2004-08-01T11:00:00Z
modified: 2015-08-31T04:07:37Z
discontinued: false
dominant: true
experience: experience/None
group u list: _4_
m user id: 0
owner id: 171
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: RhlR and C4HSL regulated promoter.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: RhlR
sequence annotation: modified P(Rhl)
sequence: https://synbiohub.org/public/igem/BBa_I14017_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation1043007
display id: annotation1043007
version: 1
top level: RhI
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I14017/annotation1043007/range1043007/1

https://synbiohub.org/public/igem/BBa_I14017/annotation1043007/range1043007/1
type: Range
persistent identity: range1043007
display id: range1043007
version: 1
direction: direction/forward
top level: RhI
start: 21
end: 50
orientation: inline

RhlR
type: SequenceAnnotation
persistent identity: annotation1043005
display id: annotation1043005
version: 1
top level: RhI
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I14017/annotation1043005/range1043005/1

https://synbiohub.org/public/igem/BBa_I14017/annotation1043005/range1043005/1
type: Range
persistent identity: range1043005
display id: range1043005
version: 1
direction: direction/forward
top level: RhI
start: 1
end: 20
orientation: inline

modified P(Rhl)
type: SequenceAnnotation
persistent identity: annotation1043006
display id: annotation1043006
version: 1
top level: RhI
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I14017/annotation1043006/range1043006/1

https://synbiohub.org/public/igem/BBa_I14017/annotation1043006/range1043006/1
type: Range
persistent identity: range1043006
display id: range1043006
version: 1
direction: direction/forward
top level: RhI
start: 1
end: 51
orientation: inline

PnisA
type: ComponentDefinition
persistent identity: BBa K1830001
display id: BBa_K1830001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PnisA
creator: Mathieu Husser
created: 2015-08-05T11:00:00Z
modified: 2015-08-07T11:50:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2256_
m user id: 27439
owner id: 27439
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Promoter and ribosome binding site of the Lactococcal nisin inducible operon.
mutable notes: Small part
mutable provenance: L. Lactis NisA operon
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: RBS
sequence annotation: -35
sequence annotation: PnisA
sequence: https://synbiohub.org/public/igem/BBa_K1830001_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation2434540
display id: annotation2434540
version: 1
top level: PnisA
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1830001/annotation2434540/range2434540/1

https://synbiohub.org/public/igem/BBa_K1830001/annotation2434540/range2434540/1
type: Range
persistent identity: range2434540
display id: range2434540
version: 1
direction: direction/forward
top level: PnisA
start: 120
end: 125
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2434538
display id: annotation2434538
version: 1
top level: PnisA
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1830001/annotation2434538/range2434538/1

https://synbiohub.org/public/igem/BBa_K1830001/annotation2434538/range2434538/1
type: Range
persistent identity: range2434538
display id: range2434538
version: 1
direction: direction/forward
top level: PnisA
start: 159
end: 165
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2434539
display id: annotation2434539
version: 1
top level: PnisA
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1830001/annotation2434539/range2434539/1

https://synbiohub.org/public/igem/BBa_K1830001/annotation2434539/range2434539/1
type: Range
persistent identity: range2434539
display id: range2434539
version: 1
direction: direction/forward
top level: PnisA
start: 97
end: 102
orientation: inline

PnisA
type: SequenceAnnotation
persistent identity: annotation2434537
display id: annotation2434537
version: 1
top level: PnisA
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1830001/annotation2434537/range2434537/1

https://synbiohub.org/public/igem/BBa_K1830001/annotation2434537/range2434537/1
type: Range
persistent identity: range2434537
display id: range2434537
version: 1
direction: direction/forward
top level: PnisA
start: 1
end: 173
orientation: inline

BBa_K121011
type: ComponentDefinition
persistent identity: BBa K121011
display id: BBa_K121011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: promoter (lacI regulated)
creator: Masashi Kajita
created: 2008-10-15T11:00:00Z
modified: 2015-05-08T01:09:41Z
discontinued: false
dominant: false
group u list: _220_
m user id: 0
owner id: 3180
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: pressure inducible lac promoter
mutable notes: na
mutable provenance: na
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: K121011
sequence annotation: -10
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: LacI binding site
sequence: https://synbiohub.org/public/igem/BBa_K121011_sequence/1

K121011
type: SequenceAnnotation
persistent identity: annotation1981858
display id: annotation1981858
version: 1
top level: BBa_K121011
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K121011/annotation1981858/range1981858/1

https://synbiohub.org/public/igem/BBa_K121011/annotation1981858/range1981858/1
type: Range
persistent identity: range1981858
display id: range1981858
version: 1
direction: direction/forward
top level: BBa_K121011
start: 1
end: 232
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1981861
display id: annotation1981861
version: 1
top level: BBa_K121011
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K121011/annotation1981861/range1981861/1

https://synbiohub.org/public/igem/BBa_K121011/annotation1981861/range1981861/1
type: Range
persistent identity: range1981861
display id: range1981861
version: 1
direction: direction/forward
top level: BBa_K121011
start: 165
end: 168
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1981859
display id: annotation1981859
version: 1
top level: BBa_K121011
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K121011/annotation1981859/range1981859/1

https://synbiohub.org/public/igem/BBa_K121011/annotation1981859/range1981859/1
type: Range
persistent identity: range1981859
display id: range1981859
version: 1
direction: direction/forward
top level: BBa_K121011
start: 94
end: 131
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1981860
display id: annotation1981860
version: 1
top level: BBa_K121011
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K121011/annotation1981860/range1981860/1

https://synbiohub.org/public/igem/BBa_K121011/annotation1981860/range1981860/1
type: Range
persistent identity: range1981860
display id: range1981860
version: 1
direction: direction/forward
top level: BBa_K121011
start: 141
end: 146
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1981862
display id: annotation1981862
version: 1
top level: BBa_K121011
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K121011/annotation1981862/range1981862/1

https://synbiohub.org/public/igem/BBa_K121011/annotation1981862/range1981862/1
type: Range
persistent identity: range1981862
display id: range1981862
version: 1
direction: direction/forward
top level: BBa_K121011
start: 169
end: 203
orientation: inline

BBa_I13453
type: ComponentDefinition
persistent identity: BBa I13453
display id: BBa_I13453
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pbad promoter
creator: jkm
created: 2005-05-24T11:00:00Z
modified: 2015-08-31T04:07:34Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: PBad promoter from I0500 without AraC.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I13453_sequence/1

BBa_J06403
type: ComponentDefinition
persistent identity: BBa J06403
display id: BBa_J06403
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RhIR promoter repressible by CI
creator: pbradley
created: 2005-06-23T11:00:00Z
modified: 2015-08-31T04:08:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _20_
m user id: 0
owner id: 358
owning group id: 20
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: THIS PART DOES NOT WORK. Need to add the CI operator sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: modified P(Rhl)
sequence annotation: RhlR
sequence annotation: BBa_I14017
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_J06403_sequence/1

modified P(Rhl)
type: SequenceAnnotation
persistent identity: annotation1538365
display id: annotation1538365
version: 1
top level: BBa_J06403
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_J06403/annotation1538365/range1538365/1

https://synbiohub.org/public/igem/BBa_J06403/annotation1538365/range1538365/1
type: Range
persistent identity: range1538365
display id: range1538365
version: 1
direction: direction/forward
top level: BBa_J06403
start: 1
end: 51
orientation: inline

RhlR
type: SequenceAnnotation
persistent identity: annotation1538366
display id: annotation1538366
version: 1
top level: BBa_J06403
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J06403/annotation1538366/range1538366/1

https://synbiohub.org/public/igem/BBa_J06403/annotation1538366/range1538366/1
type: Range
persistent identity: range1538366
display id: range1538366
version: 1
direction: direction/forward
top level: BBa_J06403
start: 1
end: 20
orientation: inline

BBa_I14017
type: SequenceAnnotation
persistent identity: annotation1538368
display id: annotation1538368
version: 1
top level: BBa_J06403
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J06403/annotation1538368/range1538368/1

https://synbiohub.org/public/igem/BBa_J06403/annotation1538368/range1538368/1
type: Range
persistent identity: range1538368
display id: range1538368
version: 1
direction: direction/forward
top level: BBa_J06403
start: 1
end: 51
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1538367
display id: annotation1538367
version: 1
top level: BBa_J06403
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J06403/annotation1538367/range1538367/1

https://synbiohub.org/public/igem/BBa_J06403/annotation1538367/range1538367/1
type: Range
persistent identity: range1538367
display id: range1538367
version: 1
direction: direction/forward
top level: BBa_J06403
start: 21
end: 50
orientation: inline

BBa_K1946001
type: ComponentDefinition
persistent identity: BBa K1946001
display id: BBa_K1946001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pChnB
creator: Musa Efe Işılak
created: 2016-10-12T11:00:00Z
modified: 2016-10-13T05:03:54Z
discontinued: false
dominant: false
group u list: _2413_
m user id: 29843
owner id: 29843
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part is the region starting from 537bp upstream of ChnB to its start codon from Acinetobacter sp. cyclohenol gene cluster. pChnB is activated by ChnR (K1946000) in the presence of cyclohexanone. Iwaki H, Hasegawa Y, Teraoka M, Tokuyama T, Bergeron H, Lau PC. Identification of a transcriptional activator (ChnR) and a 6-oxohexanoate dehydrogenase (ChnE) in the cyclohexanol catabolic pathway in Acinetobacter sp. Strain NCIMB 9871 and localization of the genes that encode them. Appl Environ Microbiol. 1999;65(11):5158-62. Steigedal, Magnus and Svein Valla. "The Acinetobacter Sp. Chnb Promoter Together With Its Cognate Positive Regulator Chnr Is An Attractive New Candidate For Metabolic Engineering Applications In Bacteria". Metabolic Engineering 10.2 (2008): 121-129. Web.
mutable notes: The exact region where ChnR binds is not known.
mutable provenance: This part is from the first 537bp of GenBank: AB006902.2
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: promoter
sequence: https://synbiohub.org/public/igem/BBa_K1946001_sequence/1

promoter
type: SequenceAnnotation
persistent identity: annotation2497224
display id: annotation2497224
version: 1
top level: BBa_K1946001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1946001/annotation2497224/range2497224/1

https://synbiohub.org/public/igem/BBa_K1946001/annotation2497224/range2497224/1
type: Range
persistent identity: range2497224
display id: range2497224
version: 1
direction: direction/forward
top level: BBa_K1946001
start: 1
end: 537
orientation: inline

BBa_K284002
type: ComponentDefinition
persistent identity: BBa K284002
display id: BBa_K284002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: JEN1 Promoter from Kluyveromyces lactis
creator: Raissa Estrela Curado, Tais Sineiro Herig and Gleidson Silva Teixeira
created: 2009-09-25T11:00:00Z
modified: 2015-05-08T01:11:48Z
discontinued: false
dominant: false
experience: experience/None
group u list: _386_
m user id: 0
owner id: 4968
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: .
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: primer_binding
sequence annotation: TATA-Like
sequence annotation: JEN1 Promoter
sequence: https://synbiohub.org/public/igem/BBa_K284002_sequence/1

primer_binding
type: SequenceAnnotation
persistent identity: annotation2034977
display id: annotation2034977
version: 1
top level: BBa_K284002
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K284002/annotation2034977/range2034977/1

https://synbiohub.org/public/igem/BBa_K284002/annotation2034977/range2034977/1
type: Range
persistent identity: range2034977
display id: range2034977
version: 1
direction: direction/forward
top level: BBa_K284002
start: 1
end: 19
orientation: inline

TATA-Like
type: SequenceAnnotation
persistent identity: annotation2040986
display id: annotation2040986
version: 1
top level: BBa_K284002
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K284002/annotation2040986/range2040986/1

https://synbiohub.org/public/igem/BBa_K284002/annotation2040986/range2040986/1
type: Range
persistent identity: range2040986
display id: range2040986
version: 1
direction: direction/forward
top level: BBa_K284002
start: 862
end: 873
orientation: inline

primer_binding
type: SequenceAnnotation
persistent identity: annotation2034979
display id: annotation2034979
version: 1
top level: BBa_K284002
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K284002/annotation2034979/range2034979/1

https://synbiohub.org/public/igem/BBa_K284002/annotation2034979/range2034979/1
type: Range
persistent identity: range2034979
display id: range2034979
version: 1
direction: direction/reverse
top level: BBa_K284002
start: 976
end: 998
orientation: inline

JEN1 Promoter
type: SequenceAnnotation
persistent identity: annotation2034978
display id: annotation2034978
version: 1
top level: BBa_K284002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K284002/annotation2034978/range2034978/1

https://synbiohub.org/public/igem/BBa_K284002/annotation2034978/range2034978/1
type: Range
persistent identity: range2034978
display id: range2034978
version: 1
direction: direction/forward
top level: BBa_K284002
start: 1
end: 998
orientation: inline

BBa_K598001
type: ComponentDefinition
persistent identity: BBa K598001
display id: BBa_K598001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Theophylline Responsive Riboswitch 1G1 with Engineered RBS+GFP generator
creator: Siyang HAO, Tong MU, Zheng LUO, Mengqi ZHANG
created: 2011-09-26T11:00:00Z
modified: 2015-05-08T01:12:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _770_
m user id: 0
owner id: 8713
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is a GFP generator with a theophylline responsive riboswitch sequence. The mRNA with a theophylline riboswitch in it would respond to theophylline concerntration, producing different amount of GFP. Under the control of different promoters, it could be used to demonstrate the theophylline responsive curve, providing data for modeling. The theophylline riboswitch sequence could also be used to regulate the translation of other genes.
mutable notes: The RBS of this device is altered into AGGAGGU. When changing the RBS, the anti-RBS sequence should be changed as well.
mutable provenance: Theophylline riboswich is composed of a synthesized theophylline aptamer and a regulatory platform.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: stop
sequence annotation: Engineered RBS
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: Theophylline responsive riboswitch Theo 1G1
sequence annotation: BBa_E0040
sequence annotation: T7 TE
sequence annotation: stem_loop
sequence annotation: polya
sequence annotation: eGFP
sequence: https://synbiohub.org/public/igem/BBa_K598001_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2152892
display id: annotation2152892
version: 1
top level: BBa_K598001
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K598001/annotation2152892/range2152892/1

https://synbiohub.org/public/igem/BBa_K598001/annotation2152892/range2152892/1
type: Range
persistent identity: range2152892
display id: range2152892
version: 1
direction: direction/forward
top level: BBa_K598001
start: 936
end: 936
orientation: inline

Engineered RBS
type: SequenceAnnotation
persistent identity: annotation2152702
display id: annotation2152702
version: 1
top level: BBa_K598001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598001/annotation2152702/range2152702/1

https://synbiohub.org/public/igem/BBa_K598001/annotation2152702/range2152702/1
type: Range
persistent identity: range2152702
display id: range2152702
version: 1
direction: direction/forward
top level: BBa_K598001
start: 75
end: 81
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2152882
display id: annotation2152882
version: 1
top level: BBa_K598001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K598001/annotation2152882/range2152882/1

https://synbiohub.org/public/igem/BBa_K598001/annotation2152882/range2152882/1
type: Range
persistent identity: range2152882
display id: range2152882
version: 1
direction: direction/forward
top level: BBa_K598001
start: 815
end: 894
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2152705
display id: annotation2152705
version: 1
top level: BBa_K598001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K598001/annotation2152705/range2152705/1

https://synbiohub.org/public/igem/BBa_K598001/annotation2152705/range2152705/1
type: Range
persistent identity: range2152705
display id: range2152705
version: 1
direction: direction/forward
top level: BBa_K598001
start: 903
end: 943
orientation: inline

Theophylline responsive riboswitch Theo 1G1
type: SequenceAnnotation
persistent identity: annotation2152701
display id: annotation2152701
version: 1
top level: BBa_K598001
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K598001/annotation2152701/range2152701/1

https://synbiohub.org/public/igem/BBa_K598001/annotation2152701/range2152701/1
type: Range
persistent identity: range2152701
display id: range2152701
version: 1
direction: direction/forward
top level: BBa_K598001
start: 1
end: 86
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2152703
display id: annotation2152703
version: 1
top level: BBa_K598001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K598001/annotation2152703/range2152703/1

https://synbiohub.org/public/igem/BBa_K598001/annotation2152703/range2152703/1
type: Range
persistent identity: range2152703
display id: range2152703
version: 1
direction: direction/forward
top level: BBa_K598001
start: 87
end: 806
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2152901
display id: annotation2152901
version: 1
top level: BBa_K598001
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K598001/annotation2152901/range2152901/1

https://synbiohub.org/public/igem/BBa_K598001/annotation2152901/range2152901/1
type: Range
persistent identity: range2152901
display id: range2152901
version: 1
direction: direction/forward
top level: BBa_K598001
start: 910
end: 929
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2152895
display id: annotation2152895
version: 1
top level: BBa_K598001
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K598001/annotation2152895/range2152895/1

https://synbiohub.org/public/igem/BBa_K598001/annotation2152895/range2152895/1
type: Range
persistent identity: range2152895
display id: range2152895
version: 1
direction: direction/forward
top level: BBa_K598001
start: 826
end: 869
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation2152887
display id: annotation2152887
version: 1
top level: BBa_K598001
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K598001/annotation2152887/range2152887/1

https://synbiohub.org/public/igem/BBa_K598001/annotation2152887/range2152887/1
type: Range
persistent identity: range2152887
display id: range2152887
version: 1
direction: direction/forward
top level: BBa_K598001
start: 930
end: 943
orientation: inline

eGFP
type: SequenceAnnotation
persistent identity: annotation2152704
display id: annotation2152704
version: 1
top level: BBa_K598001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K598001/annotation2152704/range2152704/1

https://synbiohub.org/public/igem/BBa_K598001/annotation2152704/range2152704/1
type: Range
persistent identity: range2152704
display id: range2152704
version: 1
direction: direction/forward
top level: BBa_K598001
start: 87
end: 806
orientation: inline

BBa_J05216
type: ComponentDefinition
persistent identity: BBa J05216
display id: BBa_J05216
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Regulator for R3-ATF6
creator: Alexander Roth
created: 2005-09-07T11:00:00Z
modified: 2015-08-31T04:08:15Z
discontinued: false
dominant: false
group u list: _34_
m user id: 0
owner id: 412
owning group id: 34
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: binding sites for R1-CREBH and R4-cMaf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J05216_sequence/1

BBa_K145150
type: ComponentDefinition
persistent identity: BBa K145150
display id: BBa_K145150
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Hybrid promoter: HSL-LuxR activated, P22 C2 repressed
creator: Jonas Demeulemeester
created: 2008-08-05T11:00:00Z
modified: 2015-05-08T01:10:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _257_
m user id: 0
owner id: 2970
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Hybrid promoter consisting of the Lux box which overlaps partly with -35 box. Binding sites for P22 C2 OR2 and OR1 are located between the -35 and -10 boxes and downstream of them respectively.
mutable notes: -35 box is partly composite. -10 is taken from the P22 phage PR regulatory region
mutable provenance: Synthetic
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Lux-box
sequence annotation: OR2
sequence annotation: -35
sequence annotation: OR1
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_K145150_sequence/1

Lux-box
type: SequenceAnnotation
persistent identity: annotation1971582
display id: annotation1971582
version: 1
top level: BBa_K145150
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K145150/annotation1971582/range1971582/1

https://synbiohub.org/public/igem/BBa_K145150/annotation1971582/range1971582/1
type: Range
persistent identity: range1971582
display id: range1971582
version: 1
direction: direction/forward
top level: BBa_K145150
start: 1
end: 20
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation1971584
display id: annotation1971584
version: 1
top level: BBa_K145150
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K145150/annotation1971584/range1971584/1

https://synbiohub.org/public/igem/BBa_K145150/annotation1971584/range1971584/1
type: Range
persistent identity: range1971584
display id: range1971584
version: 1
direction: direction/forward
top level: BBa_K145150
start: 23
end: 40
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1971583
display id: annotation1971583
version: 1
top level: BBa_K145150
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K145150/annotation1971583/range1971583/1

https://synbiohub.org/public/igem/BBa_K145150/annotation1971583/range1971583/1
type: Range
persistent identity: range1971583
display id: range1971583
version: 1
direction: direction/forward
top level: BBa_K145150
start: 20
end: 25
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation1971586
display id: annotation1971586
version: 1
top level: BBa_K145150
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K145150/annotation1971586/range1971586/1

https://synbiohub.org/public/igem/BBa_K145150/annotation1971586/range1971586/1
type: Range
persistent identity: range1971586
display id: range1971586
version: 1
direction: direction/forward
top level: BBa_K145150
start: 46
end: 63
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1971585
display id: annotation1971585
version: 1
top level: BBa_K145150
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K145150/annotation1971585/range1971585/1

https://synbiohub.org/public/igem/BBa_K145150/annotation1971585/range1971585/1
type: Range
persistent identity: range1971585
display id: range1971585
version: 1
direction: direction/forward
top level: BBa_K145150
start: 42
end: 47
orientation: inline

BBa_I741020
type: ComponentDefinition
persistent identity: BBa I741020
display id: BBa_I741020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: promoter to xylF without CRP and several binding sites for xylR
creator: Lucien Weiss
created: 2007-06-20T11:00:00Z
modified: 2015-08-31T04:08:02Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _143_
m user id: 0
owner id: 1763
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: removed RBS crp binding and xylR binding sites
mutable provenance: ecoli k12 chromosome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: XylR-Xylose transcriptional activator activates transcription
sequence annotation: Transcription start base
sequence annotation: xylFp
sequence: https://synbiohub.org/public/igem/BBa_I741020_sequence/1

XylR-Xylose transcriptional activator activates transcription
type: SequenceAnnotation
persistent identity: annotation1935180
display id: annotation1935180
version: 1
top level: BBa_I741020
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I741020/annotation1935180/range1935180/1

https://synbiohub.org/public/igem/BBa_I741020/annotation1935180/range1935180/1
type: Range
persistent identity: range1935180
display id: range1935180
version: 1
direction: direction/forward
top level: BBa_I741020
start: 95
end: 112
orientation: inline

XylR-Xylose transcriptional activator activates transcription
type: SequenceAnnotation
persistent identity: annotation1935182
display id: annotation1935182
version: 1
top level: BBa_I741020
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I741020/annotation1935182/range1935182/1

https://synbiohub.org/public/igem/BBa_I741020/annotation1935182/range1935182/1
type: Range
persistent identity: range1935182
display id: range1935182
version: 1
direction: direction/forward
top level: BBa_I741020
start: 116
end: 132
orientation: inline

Transcription start base
type: SequenceAnnotation
persistent identity: annotation1935183
display id: annotation1935183
version: 1
top level: BBa_I741020
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I741020/annotation1935183/range1935183/1

https://synbiohub.org/public/igem/BBa_I741020/annotation1935183/range1935183/1
type: Range
persistent identity: range1935183
display id: range1935183
version: 1
direction: direction/forward
top level: BBa_I741020
start: 165
end: 165
orientation: inline

xylFp
type: SequenceAnnotation
persistent identity: annotation1935181
display id: annotation1935181
version: 1
top level: BBa_I741020
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I741020/annotation1935181/range1935181/1

https://synbiohub.org/public/igem/BBa_I741020/annotation1935181/range1935181/1
type: Range
persistent identity: range1935181
display id: range1935181
version: 1
direction: direction/forward
top level: BBa_I741020
start: 105
end: 185
orientation: inline

BBa_K136003
type: ComponentDefinition
persistent identity: BBa K136003
display id: BBa_K136003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter of flhB gene from E. coli flagella
creator: Cyprien Maisonnier
created: 2008-08-11T11:00:00Z
modified: 2015-05-08T01:10:02Z
discontinued: true
dominant: false
experience: experience/None
group u list: _211_
m user id: 0
owner id: 3012
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: ''flhB'' is a class 2 gene that is part of the construction of the flagella ( ''Ordering Genes in a Flagella Pathway by Analysis of Expression Kinetics from Living Bacteria'' - S. Kalir, J. McClure, K. Pabbaraju, C. Southward, M. Ronen, S. Leibler, M. G. Surette, U. Alon ). Its promoter is consitutively repressed. It is activated by FlhD4C2 and maybe by FliA with different strength ( ''Using a Quantitative Blueprint to Reprogram the Dynamics of the Flagella Gene Network'' - Shiraz Kalir and Uri Alon ). '''Work in progress :''' Deeper quantitative characterization
mutable notes: The primers used to amplify this sequence are : *Forward : GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCCACGTCATATCAGGCGGTCTGATAAGG *Reverse : GTTTCTTCCTGCAGCGGCCGCTACTAGTAGTTTTGTCGTCGCTCTCGTCAGACACGTC
mutable provenance: This part comes from the genome of ''E.coli'' K12 strain MG1655.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Initiation for Transcription
sequence annotation: FlhDC Binding site 2
sequence annotation: FlhDC Binding site 1
sequence: https://synbiohub.org/public/igem/BBa_K136003_sequence/1

Initiation for Transcription
type: SequenceAnnotation
persistent identity: annotation1971576
display id: annotation1971576
version: 1
top level: BBa_K136003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K136003/annotation1971576/range1971576/1

https://synbiohub.org/public/igem/BBa_K136003/annotation1971576/range1971576/1
type: Range
persistent identity: range1971576
display id: range1971576
version: 1
direction: direction/forward
top level: BBa_K136003
start: 153
end: 153
orientation: inline

FlhDC Binding site 2
type: SequenceAnnotation
persistent identity: annotation1971575
display id: annotation1971575
version: 1
top level: BBa_K136003
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K136003/annotation1971575/range1971575/1

https://synbiohub.org/public/igem/BBa_K136003/annotation1971575/range1971575/1
type: Range
persistent identity: range1971575
display id: range1971575
version: 1
direction: direction/forward
top level: BBa_K136003
start: 106
end: 121
orientation: inline

FlhDC Binding site 1
type: SequenceAnnotation
persistent identity: annotation1971574
display id: annotation1971574
version: 1
top level: BBa_K136003
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K136003/annotation1971574/range1971574/1

https://synbiohub.org/public/igem/BBa_K136003/annotation1971574/range1971574/1
type: Range
persistent identity: range1971574
display id: range1971574
version: 1
direction: direction/forward
top level: BBa_K136003
start: 79
end: 94
orientation: inline

BBa_K091107
type: ComponentDefinition
persistent identity: BBa K091107
display id: BBa_K091107
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLux/cI Hybrid Promoter
creator: Xiao Zhu
created: 2008-06-18T11:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _191_
m user id: 0
owner id: 3130
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This hybrid promoter is designed to respond to activation by LuxR when it is bound to AHL and to repression by the phage lambda cI repressor. The repression needs to win over activation.
mutable notes: This part is based on the idea of replacing the region of the Lux promoter downstream of the -35 with the OR2 cI half-operator, added LuxR-10 region. Based on what we have found up to now, promoters which are constructed just by inserting OR1/2 downstream of Lux are all very leaky.
mutable provenance: this part comes from LuxR promoter and cI lambda promoter.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: cI OR2
sequence annotation: Lux Box
sequence annotation: Lux -10
sequence annotation: Lux -35
sequence: https://synbiohub.org/public/igem/BBa_K091107_sequence/1

cI OR2
type: SequenceAnnotation
persistent identity: annotation1963752
display id: annotation1963752
version: 1
top level: BBa_K091107
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K091107/annotation1963752/range1963752/1

https://synbiohub.org/public/igem/BBa_K091107/annotation1963752/range1963752/1
type: Range
persistent identity: range1963752
display id: range1963752
version: 1
direction: direction/forward
top level: BBa_K091107
start: 26
end: 43
orientation: inline

Lux Box
type: SequenceAnnotation
persistent identity: annotation1963750
display id: annotation1963750
version: 1
top level: BBa_K091107
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K091107/annotation1963750/range1963750/1

https://synbiohub.org/public/igem/BBa_K091107/annotation1963750/range1963750/1
type: Range
persistent identity: range1963750
display id: range1963750
version: 1
direction: direction/forward
top level: BBa_K091107
start: 1
end: 20
orientation: inline

Lux -10
type: SequenceAnnotation
persistent identity: annotation1963753
display id: annotation1963753
version: 1
top level: BBa_K091107
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K091107/annotation1963753/range1963753/1

https://synbiohub.org/public/igem/BBa_K091107/annotation1963753/range1963753/1
type: Range
persistent identity: range1963753
display id: range1963753
version: 1
direction: direction/forward
top level: BBa_K091107
start: 44
end: 49
orientation: inline

Lux -35
type: SequenceAnnotation
persistent identity: annotation1963751
display id: annotation1963751
version: 1
top level: BBa_K091107
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K091107/annotation1963751/range1963751/1

https://synbiohub.org/public/igem/BBa_K091107/annotation1963751/range1963751/1
type: Range
persistent identity: range1963751
display id: range1963751
version: 1
direction: direction/forward
top level: BBa_K091107
start: 20
end: 25
orientation: inline

BBa_K180017
type: ComponentDefinition
persistent identity: BBa K180017
display id: BBa_K180017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Rock-paper-scissors - Scissors primary plasmid
creator: Richard Wilson, Stephanie Chambers
created: 2009-10-20T11:00:00Z
modified: 2015-05-08T01:11:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _278_
m user id: 0
owner id: 4082
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: Composite part, consisting of BBa_K180018 and BBa_K180019, but with BBa_K180018 reading along the reverse strand, and BBa_K180019 reading along the forward strand. Forms the 'scissors' primary plasmid in Team:Southampton's rock-paper-scissors project design.
mutable notes: BBa_K180018 reading along reverse strand, and BBa_K180019 reading along the forward strand to ensure the constitutive promoter BBa_J23119 (present in BBa_K180018) has no effect over mBanana or LuxI expression in BBa_K180019.
mutable provenance: See relevant biobrick component parts (BBa_K180018 and BBa_K180019).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: mBanana
sequence annotation: conserved
sequence annotation: -35
sequence annotation: B0015
sequence annotation: B0034
sequence annotation: K180009
sequence annotation: K180019
sequence annotation: -10
sequence annotation: C0161
sequence annotation: C0179
sequence annotation: J23119
sequence annotation: lasR
sequence annotation: K180018
sequence annotation: Ptac
sequence annotation: lacI
sequence annotation: K180000
sequence annotation: lacIq
sequence: https://synbiohub.org/public/igem/BBa_K180017_sequence/1

mBanana
type: SequenceAnnotation
persistent identity: annotation2058470
display id: annotation2058470
version: 1
top level: BBa_K180017
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058470/range2058470/1

https://synbiohub.org/public/igem/BBa_K180017/annotation2058470/range2058470/1
type: Range
persistent identity: range2058470
display id: range2058470
version: 1
direction: direction/forward
top level: BBa_K180017
start: 2403
end: 3110
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2058458
display id: annotation2058458
version: 1
top level: BBa_K180017
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058458/range2058458/1

https://synbiohub.org/public/igem/BBa_K180017/annotation2058458/range2058458/1
type: Range
persistent identity: range2058458
display id: range2058458
version: 1
direction: direction/reverse
top level: BBa_K180017
start: 871
end: 874
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2058461
display id: annotation2058461
version: 1
top level: BBa_K180017
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058461/range2058461/1

https://synbiohub.org/public/igem/BBa_K180017/annotation2058461/range2058461/1
type: Range
persistent identity: range2058461
display id: range2058461
version: 1
direction: direction/reverse
top level: BBa_K180017
start: 916
end: 921
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2058453
display id: annotation2058453
version: 1
top level: BBa_K180017
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058453/range2058453/1

https://synbiohub.org/public/igem/BBa_K180017/annotation2058453/range2058453/1
type: Range
persistent identity: range2058453
display id: range2058453
version: 1
direction: direction/reverse
top level: BBa_K180017
start: 1
end: 129
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2058468
display id: annotation2058468
version: 1
top level: BBa_K180017
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058468/range2058468/1

https://synbiohub.org/public/igem/BBa_K180017/annotation2058468/range2058468/1
type: Range
persistent identity: range2058468
display id: range2058468
version: 1
direction: direction/forward
top level: BBa_K180017
start: 2390
end: 2393
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2058457
display id: annotation2058457
version: 1
top level: BBa_K180017
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058457/range2058457/1

https://synbiohub.org/public/igem/BBa_K180017/annotation2058457/range2058457/1
type: Range
persistent identity: range2058457
display id: range2058457
version: 1
direction: direction/reverse
top level: BBa_K180017
start: 867
end: 878
orientation: inline

K180009
type: SequenceAnnotation
persistent identity: annotation2058469
display id: annotation2058469
version: 1
top level: BBa_K180017
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058469/range2058469/1

https://synbiohub.org/public/igem/BBa_K180017/annotation2058469/range2058469/1
type: Range
persistent identity: range2058469
display id: range2058469
version: 1
direction: direction/forward
top level: BBa_K180017
start: 2403
end: 3110
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2058474
display id: annotation2058474
version: 1
top level: BBa_K180017
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058474/range2058474/1

https://synbiohub.org/public/igem/BBa_K180017/annotation2058474/range2058474/1
type: Range
persistent identity: range2058474
display id: range2058474
version: 1
direction: direction/forward
top level: BBa_K180017
start: 3730
end: 3858
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2058471
display id: annotation2058471
version: 1
top level: BBa_K180017
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058471/range2058471/1

https://synbiohub.org/public/igem/BBa_K180017/annotation2058471/range2058471/1
type: Range
persistent identity: range2058471
display id: range2058471
version: 1
direction: direction/forward
top level: BBa_K180017
start: 3119
end: 3130
orientation: inline

K180019
type: SequenceAnnotation
persistent identity: annotation2058464
display id: annotation2058464
version: 1
top level: BBa_K180017
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058464/range2058464/1

https://synbiohub.org/public/igem/BBa_K180017/annotation2058464/range2058464/1
type: Range
persistent identity: range2058464
display id: range2058464
version: 1
direction: direction/forward
top level: BBa_K180017
start: 930
end: 3858
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2058467
display id: annotation2058467
version: 1
top level: BBa_K180017
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058467/range2058467/1

https://synbiohub.org/public/igem/BBa_K180017/annotation2058467/range2058467/1
type: Range
persistent identity: range2058467
display id: range2058467
version: 1
direction: direction/forward
top level: BBa_K180017
start: 2389
end: 2392
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2058460
display id: annotation2058460
version: 1
top level: BBa_K180017
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058460/range2058460/1

https://synbiohub.org/public/igem/BBa_K180017/annotation2058460/range2058460/1
type: Range
persistent identity: range2058460
display id: range2058460
version: 1
direction: direction/reverse
top level: BBa_K180017
start: 893
end: 898
orientation: inline

C0161
type: SequenceAnnotation
persistent identity: annotation2058472
display id: annotation2058472
version: 1
top level: BBa_K180017
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058472/range2058472/1

https://synbiohub.org/public/igem/BBa_K180017/annotation2058472/range2058472/1
type: Range
persistent identity: range2058472
display id: range2058472
version: 1
direction: direction/forward
top level: BBa_K180017
start: 3137
end: 3721
orientation: inline

C0179
type: SequenceAnnotation
persistent identity: annotation2058456
display id: annotation2058456
version: 1
top level: BBa_K180017
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058456/range2058456/1

https://synbiohub.org/public/igem/BBa_K180017/annotation2058456/range2058456/1
type: Range
persistent identity: range2058456
display id: range2058456
version: 1
direction: direction/reverse
top level: BBa_K180017
start: 138
end: 860
orientation: inline

J23119
type: SequenceAnnotation
persistent identity: annotation2058459
display id: annotation2058459
version: 1
top level: BBa_K180017
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058459/range2058459/1

https://synbiohub.org/public/igem/BBa_K180017/annotation2058459/range2058459/1
type: Range
persistent identity: range2058459
display id: range2058459
version: 1
direction: direction/reverse
top level: BBa_K180017
start: 887
end: 921
orientation: inline

lasR
type: SequenceAnnotation
persistent identity: annotation2058455
display id: annotation2058455
version: 1
top level: BBa_K180017
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058455/range2058455/1

https://synbiohub.org/public/igem/BBa_K180017/annotation2058455/range2058455/1
type: Range
persistent identity: range2058455
display id: range2058455
version: 1
direction: direction/reverse
top level: BBa_K180017
start: 138
end: 860
orientation: inline

K180018
type: SequenceAnnotation
persistent identity: annotation2058454
display id: annotation2058454
version: 1
top level: BBa_K180017
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058454/range2058454/1

https://synbiohub.org/public/igem/BBa_K180017/annotation2058454/range2058454/1
type: Range
persistent identity: range2058454
display id: range2058454
version: 1
direction: direction/reverse
top level: BBa_K180017
start: 1
end: 921
orientation: inline

Ptac
type: SequenceAnnotation
persistent identity: annotation2058465
display id: annotation2058465
version: 1
top level: BBa_K180017
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058465/range2058465/1

https://synbiohub.org/public/igem/BBa_K180017/annotation2058465/range2058465/1
type: Range
persistent identity: range2058465
display id: range2058465
version: 1
direction: direction/forward
top level: BBa_K180017
start: 2254
end: 2281
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2058466
display id: annotation2058466
version: 1
top level: BBa_K180017
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058466/range2058466/1

https://synbiohub.org/public/igem/BBa_K180017/annotation2058466/range2058466/1
type: Range
persistent identity: range2058466
display id: range2058466
version: 1
direction: direction/forward
top level: BBa_K180017
start: 2385
end: 2396
orientation: inline

lacI
type: SequenceAnnotation
persistent identity: annotation2058473
display id: annotation2058473
version: 1
top level: BBa_K180017
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058473/range2058473/1

https://synbiohub.org/public/igem/BBa_K180017/annotation2058473/range2058473/1
type: Range
persistent identity: range2058473
display id: range2058473
version: 1
direction: direction/forward
top level: BBa_K180017
start: 3137
end: 3721
orientation: inline

K180000
type: SequenceAnnotation
persistent identity: annotation2058463
display id: annotation2058463
version: 1
top level: BBa_K180017
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058463/range2058463/1

https://synbiohub.org/public/igem/BBa_K180017/annotation2058463/range2058463/1
type: Range
persistent identity: range2058463
display id: range2058463
version: 1
direction: direction/forward
top level: BBa_K180017
start: 930
end: 2376
orientation: inline

lacIq
type: SequenceAnnotation
persistent identity: annotation2058462
display id: annotation2058462
version: 1
top level: BBa_K180017
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058462/range2058462/1

https://synbiohub.org/public/igem/BBa_K180017/annotation2058462/range2058462/1
type: Range
persistent identity: range2058462
display id: range2058462
version: 1
direction: direction/forward
top level: BBa_K180017
start: 930
end: 2011
orientation: inline

HSP-LacI
type: ComponentDefinition
persistent identity: BBa K338002
display id: BBa_K338002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: K338001+R0011: Heat Shock Promoter + LacI Regulated Promoter
creator: 2010 Caltech iGEM Team
created: 2010-10-23T11:00:00Z
modified: 2015-05-08T01:12:07Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _454_
m user id: 0
owner id: 6189
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Fused heat shock promoter with lacI regulated promoter. It possesses the characteristic of both heat shock promoter and LacI.
mutable notes: It was designed to address the issue of a leaky promoter in front of a bacterial gene in an effort to create a genetic switch just for growth/cloning
mutable provenance: containing K338001 and R0011
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K338001
component: BBa_B0034
component: BBa_R0011
sequence annotation: BBa_R0011
sequence annotation: BBa_B0034
sequence annotation: BBa_K338001
sequence: https://synbiohub.org/public/igem/BBa_K338002_sequence/1

BBa_K338001
type: Component
persistent identity: component2104035
display id: component2104035
version: 1
top level: HSP-LacI
access: public
definition: HSP

BBa_B0034
type: Component
persistent identity: component2104042
display id: component2104042
version: 1
top level: HSP-LacI
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0011
type: Component
persistent identity: component2104036
display id: component2104036
version: 1
top level: HSP-LacI
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2104036
display id: annotation2104036
version: 1
top level: HSP-LacI
location: https://synbiohub.org/public/igem/BBa_K338002/annotation2104036/range2104036/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_K338002/annotation2104036/range2104036/1
type: Range
persistent identity: range2104036
display id: range2104036
version: 1
direction: direction/forward
top level: HSP-LacI
start: 107
end: 160
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2104042
display id: annotation2104042
version: 1
top level: HSP-LacI
location: https://synbiohub.org/public/igem/BBa_K338002/annotation2104042/range2104042/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K338002/annotation2104042/range2104042/1
type: Range
persistent identity: range2104042
display id: range2104042
version: 1
direction: direction/reverse
top level: HSP-LacI
start: 170
end: 181
orientation: inline

BBa_K338001
type: SequenceAnnotation
persistent identity: annotation2104035
display id: annotation2104035
version: 1
top level: HSP-LacI
location: https://synbiohub.org/public/igem/BBa_K338002/annotation2104035/range2104035/1
component: BBa_K338001

https://synbiohub.org/public/igem/BBa_K338002/annotation2104035/range2104035/1
type: Range
persistent identity: range2104035
display id: range2104035
version: 1
direction: direction/reverse
top level: HSP-LacI
start: 1
end: 98
orientation: inline

BBa_K1660005
type: ComponentDefinition
persistent identity: BBa K1660005
display id: BBa_K1660005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RFP controlled by the PompR promoter
creator: Dai Yuanyi
created: 2015-09-07T11:00:00Z
modified: 2015-09-08T12:26:12Z
discontinued: false
dominant: false
group u list: _2078_
m user id: 26933
owner id: 26933
owning group id: 9
status: status/Planning
bookmark: false
mutable description: PompR is a OmpR-controlled promoter which can be positively regulated by phosphorylated OmpR. This promoter is taken from the upstream region of ompC. Phosphorylated OmpR binds to the three operator sites and activates transcription. We insert PompR(BBa_R0082) sequences into the upstream region of rfp gene, which together is connected to vector PSB1C3 afterwards, and therefore we are able to observe the OmpR promoter efficiency through the control of light.
mutable notes: We constructed this biobrick through pcr.
mutable provenance: This part comes from the stock.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_R0082
component: BBa_K516032
sequence annotation: BBa_R0082
sequence annotation: BBa_K516032
sequence: https://synbiohub.org/public/igem/BBa_K1660005_sequence/1

BBa_R0082
type: Component
persistent identity: component2446043
display id: component2446043
version: 1
top level: BBa_K1660005
access: public
definition: OmpR

BBa_K516032
type: Component
persistent identity: component2446057
display id: component2446057
version: 1
top level: BBa_K1660005
access: public
definition: https://synbiohub.org/public/igem/BBa_K516032/1

BBa_R0082
type: SequenceAnnotation
persistent identity: annotation2446043
display id: annotation2446043
version: 1
top level: BBa_K1660005
location: https://synbiohub.org/public/igem/BBa_K1660005/annotation2446043/range2446043/1
component: BBa_R0082

https://synbiohub.org/public/igem/BBa_K1660005/annotation2446043/range2446043/1
type: Range
persistent identity: range2446043
display id: range2446043
version: 1
direction: direction/reverse
top level: BBa_K1660005
start: 1
end: 108
orientation: inline

BBa_K516032
type: SequenceAnnotation
persistent identity: annotation2446057
display id: annotation2446057
version: 1
top level: BBa_K1660005
location: https://synbiohub.org/public/igem/BBa_K1660005/annotation2446057/range2446057/1
component: BBa_K516032

https://synbiohub.org/public/igem/BBa_K1660005/annotation2446057/range2446057/1
type: Range
persistent identity: range2446057
display id: range2446057
version: 1
direction: direction/reverse
top level: BBa_K1660005
start: 117
end: 978
orientation: inline

BBa_I12040
type: ComponentDefinition
persistent identity: BBa I12040
display id: BBa_I12040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Modified lambda P(RM) promoter: -10 region from P(L) and cooperatively repressed by 434 cI
creator: ryhsiao
created: 2004-08-18T11:00:00Z
modified: 2015-08-31T04:07:31Z
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 148
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Lamdba P(RM) promoter modified to be activated by lamda repressor (cI) and repressed by 434 repressor (cI). Two operator sites are used for both activation and repression to enhance cooperativity. Additionally, the -10 region from lambda P(L) was used instead of that from P(RM).
mutable notes: The O-R3 site of lambda (17 nt) was replaced by the O-R1 site of 434 (14 nt) to facilitate repression by 434 cI. The new 434 O-R1 is 5'-justified with the lambda O-R3 it replaced, leaving the -10 sequence intact. The 434 O-R2 site replaces the wildtype labmda promoter starting at -5, thereby preserving the 8 nt between 434 O-R1 and O-R2 in the wildtype. The mRNA transcript will contain an additional 9 nt compared to wildtype lambda phage. This part differs from BBa_I12036 because the -10 region from P(L) was used instead of that from P(RM).
mutable provenance: Bushman, F. D. The bacteriophage 434 right operator roles of O-R1, O-R2, and O-R3. J. Mol. Biol. (1993) 230, 28-40. ; Lutz, R & Bujard, H. "Independent and tight regulation of transcriptional units in Escherichia coli via the lacR/O, the TetR/O and AraC/I1-I2 regulatory elements, Nucleic Acids Research, 1997, Vol.25, No.6 (1203-1210)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OR2 434
sequence annotation: OR1 lambda
sequence annotation: OR2 lambda
sequence annotation: -35
sequence annotation: start
sequence annotation: -10
sequence annotation: OR1 434
sequence: https://synbiohub.org/public/igem/BBa_I12040_sequence/1

OR2 434
type: SequenceAnnotation
persistent identity: annotation1059303
display id: annotation1059303
version: 1
top level: BBa_I12040
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12040/annotation1059303/range1059303/1

https://synbiohub.org/public/igem/BBa_I12040/annotation1059303/range1059303/1
type: Range
persistent identity: range1059303
display id: range1059303
version: 1
direction: direction/forward
top level: BBa_I12040
start: 78
end: 91
orientation: inline

OR1 lambda
type: SequenceAnnotation
persistent identity: annotation1059298
display id: annotation1059298
version: 1
top level: BBa_I12040
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12040/annotation1059298/range1059298/1

https://synbiohub.org/public/igem/BBa_I12040/annotation1059298/range1059298/1
type: Range
persistent identity: range1059298
display id: range1059298
version: 1
direction: direction/forward
top level: BBa_I12040
start: 9
end: 25
orientation: inline

OR2 lambda
type: SequenceAnnotation
persistent identity: annotation1059299
display id: annotation1059299
version: 1
top level: BBa_I12040
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12040/annotation1059299/range1059299/1

https://synbiohub.org/public/igem/BBa_I12040/annotation1059299/range1059299/1
type: Range
persistent identity: range1059299
display id: range1059299
version: 1
direction: direction/forward
top level: BBa_I12040
start: 33
end: 49
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1059300
display id: annotation1059300
version: 1
top level: BBa_I12040
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I12040/annotation1059300/range1059300/1

https://synbiohub.org/public/igem/BBa_I12040/annotation1059300/range1059300/1
type: Range
persistent identity: range1059300
display id: range1059300
version: 1
direction: direction/forward
top level: BBa_I12040
start: 48
end: 53
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1062263
display id: annotation1062263
version: 1
top level: BBa_I12040
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I12040/annotation1062263/range1062263/1

https://synbiohub.org/public/igem/BBa_I12040/annotation1062263/range1062263/1
type: Range
persistent identity: range1062263
display id: range1062263
version: 1
direction: direction/forward
top level: BBa_I12040
start: 83
end: 83
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1059302
display id: annotation1059302
version: 1
top level: BBa_I12040
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I12040/annotation1059302/range1059302/1

https://synbiohub.org/public/igem/BBa_I12040/annotation1059302/range1059302/1
type: Range
persistent identity: range1059302
display id: range1059302
version: 1
direction: direction/forward
top level: BBa_I12040
start: 71
end: 76
orientation: inline

OR1 434
type: SequenceAnnotation
persistent identity: annotation1059301
display id: annotation1059301
version: 1
top level: BBa_I12040
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12040/annotation1059301/range1059301/1

https://synbiohub.org/public/igem/BBa_I12040/annotation1059301/range1059301/1
type: Range
persistent identity: range1059301
display id: range1059301
version: 1
direction: direction/forward
top level: BBa_I12040
start: 56
end: 69
orientation: inline

BBa_K180015
type: ComponentDefinition
persistent identity: BBa K180015
display id: BBa_K180015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Paper - Primary plasmid (part 2) [tac pR, GFP and RhlI generator]
creator: Richard Wilson, Stephanie Chambers
created: 2009-10-18T11:00:00Z
modified: 2015-05-08T01:11:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _278_
m user id: 0
owner id: 4082
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Part of the iGEM2009 Team:Southampton rock-paper-scissors 'rock' primary plasmid.
mutable notes: Regulated expression of RhlI (C0170) and GFP (E0040) by tac pR (K180000), controlled by presence of lac/IPTG or trp in the cell.
mutable provenance: See relevant basic biobrick parts (K180000, B0034, E0040, B0015).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0010
component: BBa_C0170
component: BBa_B0012
component: BBa_B0034
component: BBa_E0040
component: BBa_K180000
sequence annotation: BBa_B0034
sequence annotation: BBa_K180000
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_C0170
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_K180015_sequence/1

BBa_B0010
type: Component
persistent identity: component2053967
display id: component2053967
version: 1
top level: BBa_K180015
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_C0170
type: Component
persistent identity: component2053966
display id: component2053966
version: 1
top level: BBa_K180015
access: public
definition: https://synbiohub.org/public/igem/BBa_C0170/1

BBa_B0012
type: Component
persistent identity: component2053969
display id: component2053969
version: 1
top level: BBa_K180015
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component2053964
display id: component2053964
version: 1
top level: BBa_K180015
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0040
type: Component
persistent identity: component2053962
display id: component2053962
version: 1
top level: BBa_K180015
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_K180000
type: Component
persistent identity: component2053958
display id: component2053958
version: 1
top level: BBa_K180015
access: public
definition: BBa_K180000

BBa_B0034
type: Component
persistent identity: component2053960
display id: component2053960
version: 1
top level: BBa_K180015
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2053960
display id: annotation2053960
version: 1
top level: BBa_K180015
location: https://synbiohub.org/public/igem/BBa_K180015/annotation2053960/range2053960/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K180015/annotation2053960/range2053960/1
type: Range
persistent identity: range2053960
display id: range2053960
version: 1
direction: direction/reverse
top level: BBa_K180015
start: 1456
end: 1467
orientation: inline

BBa_K180000
type: SequenceAnnotation
persistent identity: annotation2053958
display id: annotation2053958
version: 1
top level: BBa_K180015
location: https://synbiohub.org/public/igem/BBa_K180015/annotation2053958/range2053958/1
component: BBa_K180000

https://synbiohub.org/public/igem/BBa_K180015/annotation2053958/range2053958/1
type: Range
persistent identity: range2053958
display id: range2053958
version: 1
direction: direction/reverse
top level: BBa_K180015
start: 1
end: 1447
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2053969
display id: annotation2053969
version: 1
top level: BBa_K180015
location: https://synbiohub.org/public/igem/BBa_K180015/annotation2053969/range2053969/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K180015/annotation2053969/range2053969/1
type: Range
persistent identity: range2053969
display id: range2053969
version: 1
direction: direction/forward
top level: BBa_K180015
start: 2925
end: 2965
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2053967
display id: annotation2053967
version: 1
top level: BBa_K180015
location: https://synbiohub.org/public/igem/BBa_K180015/annotation2053967/range2053967/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K180015/annotation2053967/range2053967/1
type: Range
persistent identity: range2053967
display id: range2053967
version: 1
direction: direction/forward
top level: BBa_K180015
start: 2837
end: 2916
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2053964
display id: annotation2053964
version: 1
top level: BBa_K180015
location: https://synbiohub.org/public/igem/BBa_K180015/annotation2053964/range2053964/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K180015/annotation2053964/range2053964/1
type: Range
persistent identity: range2053964
display id: range2053964
version: 1
direction: direction/reverse
top level: BBa_K180015
start: 2202
end: 2213
orientation: inline

BBa_C0170
type: SequenceAnnotation
persistent identity: annotation2053966
display id: annotation2053966
version: 1
top level: BBa_K180015
location: https://synbiohub.org/public/igem/BBa_K180015/annotation2053966/range2053966/1
component: BBa_C0170

https://synbiohub.org/public/igem/BBa_K180015/annotation2053966/range2053966/1
type: Range
persistent identity: range2053966
display id: range2053966
version: 1
direction: direction/reverse
top level: BBa_K180015
start: 2220
end: 2828
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2053962
display id: annotation2053962
version: 1
top level: BBa_K180015
location: https://synbiohub.org/public/igem/BBa_K180015/annotation2053962/range2053962/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K180015/annotation2053962/range2053962/1
type: Range
persistent identity: range2053962
display id: range2053962
version: 1
direction: direction/reverse
top level: BBa_K180015
start: 1474
end: 2193
orientation: inline

pAND
type: ComponentDefinition
persistent identity: BBa J58100
display id: BBa_J58100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AND-type promoter synergistically activated by cI and CRP
creator: Valencia iGEM 2006
created: 2006-10-25T11:00:00Z
modified: 2015-08-31T03:14:48Z
discontinued: false
dominant: false
experience: experience/None
group u list: _83_
m user id: 0
owner id: 1100
owning group id: 83
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: none
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: lam-cI OR2 binding site
sequence annotation: -10
sequence annotation: spacer from I12007 (54-70)
sequence annotation: spacer from I12007 (26-32)
sequence annotation: CRP binding site
sequence annotation: spacer from I12007 (1-11, 59-72)
sequence annotation: spacer from I12007 (77-82)
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_J58100_sequence/1

lam-cI OR2 binding site
type: SequenceAnnotation
persistent identity: annotation1908169
display id: annotation1908169
version: 1
top level: pAND
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_J58100/annotation1908169/range1908169/1

https://synbiohub.org/public/igem/BBa_J58100/annotation1908169/range1908169/1
type: Range
persistent identity: range1908169
display id: range1908169
version: 1
direction: direction/forward
top level: pAND
start: 57
end: 74
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1908179
display id: annotation1908179
version: 1
top level: pAND
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J58100/annotation1908179/range1908179/1

https://synbiohub.org/public/igem/BBa_J58100/annotation1908179/range1908179/1
type: Range
persistent identity: range1908179
display id: range1908179
version: 1
direction: direction/forward
top level: pAND
start: 95
end: 100
orientation: inline

spacer from I12007 (54-70)
type: SequenceAnnotation
persistent identity: annotation1908181
display id: annotation1908181
version: 1
top level: pAND
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J58100/annotation1908181/range1908181/1

https://synbiohub.org/public/igem/BBa_J58100/annotation1908181/range1908181/1
type: Range
persistent identity: range1908181
display id: range1908181
version: 1
direction: direction/forward
top level: pAND
start: 78
end: 94
orientation: inline

spacer from I12007 (26-32)
type: SequenceAnnotation
persistent identity: annotation1908180
display id: annotation1908180
version: 1
top level: pAND
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J58100/annotation1908180/range1908180/1

https://synbiohub.org/public/igem/BBa_J58100/annotation1908180/range1908180/1
type: Range
persistent identity: range1908180
display id: range1908180
version: 1
direction: direction/forward
top level: pAND
start: 50
end: 56
orientation: inline

CRP binding site
type: SequenceAnnotation
persistent identity: annotation1908168
display id: annotation1908168
version: 1
top level: pAND
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_J58100/annotation1908168/range1908168/1

https://synbiohub.org/public/igem/BBa_J58100/annotation1908168/range1908168/1
type: Range
persistent identity: range1908168
display id: range1908168
version: 1
direction: direction/forward
top level: pAND
start: 3
end: 24
orientation: inline

spacer from I12007 (1-11, 59-72)
type: SequenceAnnotation
persistent identity: annotation1908171
display id: annotation1908171
version: 1
top level: pAND
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J58100/annotation1908171/range1908171/1

https://synbiohub.org/public/igem/BBa_J58100/annotation1908171/range1908171/1
type: Range
persistent identity: range1908171
display id: range1908171
version: 1
direction: direction/forward
top level: pAND
start: 25
end: 49
orientation: inline

spacer from I12007 (77-82)
type: SequenceAnnotation
persistent identity: annotation1908182
display id: annotation1908182
version: 1
top level: pAND
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J58100/annotation1908182/range1908182/1

https://synbiohub.org/public/igem/BBa_J58100/annotation1908182/range1908182/1
type: Range
persistent identity: range1908182
display id: range1908182
version: 1
direction: direction/forward
top level: pAND
start: 101
end: 106
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1908178
display id: annotation1908178
version: 1
top level: pAND
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J58100/annotation1908178/range1908178/1

https://synbiohub.org/public/igem/BBa_J58100/annotation1908178/range1908178/1
type: Range
persistent identity: range1908178
display id: range1908178
version: 1
direction: direction/forward
top level: pAND
start: 72
end: 77
orientation: inline

BBa_K090504
type: ComponentDefinition
persistent identity: BBa K090504
display id: BBa_K090504
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Gram-Positive Strong Constitutive Promoter
creator: Daniel Goodman
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
group u list: _188_
m user id: 0
owner id: 3501
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a strong constitutive (always-on) promoter for use with gram-positive organisms. It requires a downstream gram-positive ribosomal binding site and coding sequence to function. We only have experience using this part in B. subtilis, but it should function in most gram-positive organisms. It has not been tested and will most likely not work in E. coli. The promoter comes from the cis-regulatory region of the uracil phosphatase in Bacillus cereus. We functionally annotated the promoter ourselves using the DBTBS database motif search feature. (dbtbs.hgc.jp)
mutable notes: This part generates high expression in gram-positive organisms, and it may be possible to generate deleterious effects on the cell if used to express certain protein products.
mutable provenance: We PCRed this part out of the pAD43-25 gram positive vector, acquired from the Bacillus Genetic Stock Center (bgsc.org), catalog number ECE166.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: putative SigA -35
sequence annotation: putative ComK binding region
sequence annotation: putative SigA -10
sequence: https://synbiohub.org/public/igem/BBa_K090504_sequence/1

putative SigA -35
type: SequenceAnnotation
persistent identity: annotation1991956
display id: annotation1991956
version: 1
top level: BBa_K090504
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K090504/annotation1991956/range1991956/1

https://synbiohub.org/public/igem/BBa_K090504/annotation1991956/range1991956/1
type: Range
persistent identity: range1991956
display id: range1991956
version: 1
direction: direction/forward
top level: BBa_K090504
start: 102
end: 108
orientation: inline

putative ComK binding region
type: SequenceAnnotation
persistent identity: annotation1991955
display id: annotation1991955
version: 1
top level: BBa_K090504
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K090504/annotation1991955/range1991955/1

https://synbiohub.org/public/igem/BBa_K090504/annotation1991955/range1991955/1
type: Range
persistent identity: range1991955
display id: range1991955
version: 1
direction: direction/forward
top level: BBa_K090504
start: 76
end: 88
orientation: inline

putative SigA -10
type: SequenceAnnotation
persistent identity: annotation1991957
display id: annotation1991957
version: 1
top level: BBa_K090504
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K090504/annotation1991957/range1991957/1

https://synbiohub.org/public/igem/BBa_K090504/annotation1991957/range1991957/1
type: Range
persistent identity: range1991957
display id: range1991957
version: 1
direction: direction/forward
top level: BBa_K090504
start: 125
end: 130
orientation: inline

BBa_I746360
type: ComponentDefinition
persistent identity: BBa I746360
display id: BBa_I746360
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PF promoter from P2 phage
creator: Stefan Milde
created: 2007-09-10T11:00:00Z
modified: 2015-08-31T04:08:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _116_
m user id: 0
owner id: 2122
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the PF promoter taken from the P2 phage genome. It is an inducible promoter that is activated by a class of activators, including P2 ogr (I746350), PSP3 pag (I746351) and phiR73 delta (I746352). These different activators should cause different levels of activity of the PF promoter.
mutable notes: no special considerations seemed necessary
mutable provenance: The original source is the P2 phage genome. For our work the DNA came from plasmids, kindly supplied to us by Prof. Calendar, University of California (Berkeley): Bacteriophage PSP3 and fR73 Activator Proteins: Analysis of Promoter Specificities; Julien and Calendar, 1996; JOURNAL OF BACTERIOLOGY, Oct. 1996, p. 5668???5675
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: PF
sequence: https://synbiohub.org/public/igem/BBa_I746360_sequence/1

PF
type: SequenceAnnotation
persistent identity: annotation1943783
display id: annotation1943783
version: 1
top level: BBa_I746360
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I746360/annotation1943783/range1943783/1

https://synbiohub.org/public/igem/BBa_I746360/annotation1943783/range1943783/1
type: Range
persistent identity: range1943783
display id: range1943783
version: 1
direction: direction/forward
top level: BBa_I746360
start: 1
end: 91
orientation: inline

BBa_K165043
type: ComponentDefinition
persistent identity: BBa K165043
display id: BBa_K165043
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Zif268-HIV binding sites + MET25 constitutive yeast promoter
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:56Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K165011
component: BBa_K165000
sequence annotation: BBa_K165000
sequence annotation: BBa_K165011
sequence: https://synbiohub.org/public/igem/BBa_K165043_sequence/1

BBa_K165011
type: Component
persistent identity: component1996569
display id: component1996569
version: 1
top level: BBa_K165043
access: public
definition: https://synbiohub.org/public/igem/BBa_K165011/1

BBa_K165000
type: Component
persistent identity: component1996570
display id: component1996570
version: 1
top level: BBa_K165043
access: public
definition: https://synbiohub.org/public/igem/BBa_K165000/1

BBa_K165000
type: SequenceAnnotation
persistent identity: annotation1996570
display id: annotation1996570
version: 1
top level: BBa_K165043
location: https://synbiohub.org/public/igem/BBa_K165043/annotation1996570/range1996570/1
component: BBa_K165000

https://synbiohub.org/public/igem/BBa_K165043/annotation1996570/range1996570/1
type: Range
persistent identity: range1996570
display id: range1996570
version: 1
direction: direction/reverse
top level: BBa_K165043
start: 55
end: 441
orientation: inline

BBa_K165011
type: SequenceAnnotation
persistent identity: annotation1996569
display id: annotation1996569
version: 1
top level: BBa_K165043
location: https://synbiohub.org/public/igem/BBa_K165043/annotation1996569/range1996569/1
component: BBa_K165011

https://synbiohub.org/public/igem/BBa_K165043/annotation1996569/range1996569/1
type: Range
persistent identity: range1996569
display id: range1996569
version: 1
direction: direction/reverse
top level: BBa_K165043
start: 1
end: 46
orientation: inline

BBa_I741018
type: ComponentDefinition
persistent identity: BBa I741018
display id: BBa_I741018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Right facing promoter (for xylF) controlled by xylR and CRP-cAMP
creator: Lucien Weiss
created: 2007-06-20T11:00:00Z
modified: 2015-08-31T04:08:01Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _143_
m user id: 0
owner id: 1763
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: potential looping feature possibly removed
mutable notes: removed RBS, left promoter, and several xylR binding sites
mutable provenance: ecoli k12 chromosome
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Promoter: xylFp
sequence annotation: xylR activator binding site
sequence annotation: +1
sequence annotation: CRP-cAMP duel regulator binding site
sequence: https://synbiohub.org/public/igem/BBa_I741018_sequence/1

Promoter: xylFp
type: SequenceAnnotation
persistent identity: annotation1935200
display id: annotation1935200
version: 1
top level: BBa_I741018
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I741018/annotation1935200/range1935200/1

https://synbiohub.org/public/igem/BBa_I741018/annotation1935200/range1935200/1
type: Range
persistent identity: range1935200
display id: range1935200
version: 1
direction: direction/forward
top level: BBa_I741018
start: 138
end: 215
orientation: inline

xylR activator binding site
type: SequenceAnnotation
persistent identity: annotation1935201
display id: annotation1935201
version: 1
top level: BBa_I741018
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I741018/annotation1935201/range1935201/1

https://synbiohub.org/public/igem/BBa_I741018/annotation1935201/range1935201/1
type: Range
persistent identity: range1935201
display id: range1935201
version: 1
direction: direction/forward
top level: BBa_I741018
start: 146
end: 162
orientation: inline

+1
type: SequenceAnnotation
persistent identity: annotation1935202
display id: annotation1935202
version: 1
top level: BBa_I741018
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I741018/annotation1935202/range1935202/1

https://synbiohub.org/public/igem/BBa_I741018/annotation1935202/range1935202/1
type: Range
persistent identity: range1935202
display id: range1935202
version: 1
direction: direction/forward
top level: BBa_I741018
start: 195
end: 195
orientation: inline

CRP-cAMP duel regulator binding site
type: SequenceAnnotation
persistent identity: annotation1935203
display id: annotation1935203
version: 1
top level: BBa_I741018
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I741018/annotation1935203/range1935203/1

https://synbiohub.org/public/igem/BBa_I741018/annotation1935203/range1935203/1
type: Range
persistent identity: range1935203
display id: range1935203
version: 1
direction: direction/forward
top level: BBa_I741018
start: 4
end: 25
orientation: inline

xylR activator binding site
type: SequenceAnnotation
persistent identity: annotation1935199
display id: annotation1935199
version: 1
top level: BBa_I741018
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I741018/annotation1935199/range1935199/1

https://synbiohub.org/public/igem/BBa_I741018/annotation1935199/range1935199/1
type: Range
persistent identity: range1935199
display id: range1935199
version: 1
direction: direction/forward
top level: BBa_I741018
start: 125
end: 142
orientation: inline

BBa_K091117
type: ComponentDefinition
persistent identity: BBa K091117
display id: BBa_K091117
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLas promoter
creator: Erin Feeney
created: 2008-06-25T11:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: true
experience: experience/None
group u list: _191_
m user id: 0
owner id: 3067
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This pLas' promoter contains the regulatory region upstream of the LasI gene in Pseudomonas aeruginosa. K091117 includes a LasR binding site as well as a -10 region modified from CATAAA to TATAAA to reflect our pLasXOR promoter design. Activation is expected to occur in the presence of the autoinducer PAI-1 and the LasR protein. The pLas' promoter serves as a control for the evaluation of the pLasXOR promoter, part BBa_K091118.
mutable notes: To minimize leaky transcription, a secondary transcriptional start site was excluded from the design.
mutable provenance: The sequence of the LasI upstream regulatory region is available at http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=176640
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35 Region
sequence annotation: LasR Binding Site
sequence annotation: Primary Transcriptional Start
sequence annotation: -10 Region
sequence: https://synbiohub.org/public/igem/BBa_K091117_sequence/1

-35 Region
type: SequenceAnnotation
persistent identity: annotation1964072
display id: annotation1964072
version: 1
top level: BBa_K091117
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K091117/annotation1964072/range1964072/1

https://synbiohub.org/public/igem/BBa_K091117/annotation1964072/range1964072/1
type: Range
persistent identity: range1964072
display id: range1964072
version: 1
direction: direction/forward
top level: BBa_K091117
start: 86
end: 91
orientation: inline

LasR Binding Site
type: SequenceAnnotation
persistent identity: annotation1964073
display id: annotation1964073
version: 1
top level: BBa_K091117
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K091117/annotation1964073/range1964073/1

https://synbiohub.org/public/igem/BBa_K091117/annotation1964073/range1964073/1
type: Range
persistent identity: range1964073
display id: range1964073
version: 1
direction: direction/forward
top level: BBa_K091117
start: 75
end: 93
orientation: inline

Primary Transcriptional Start
type: SequenceAnnotation
persistent identity: annotation1964075
display id: annotation1964075
version: 1
top level: BBa_K091117
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K091117/annotation1964075/range1964075/1

https://synbiohub.org/public/igem/BBa_K091117/annotation1964075/range1964075/1
type: Range
persistent identity: range1964075
display id: range1964075
version: 1
direction: direction/reverse
top level: BBa_K091117
start: 125
end: 125
orientation: inline

-10 Region
type: SequenceAnnotation
persistent identity: annotation1964074
display id: annotation1964074
version: 1
top level: BBa_K091117
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K091117/annotation1964074/range1964074/1

https://synbiohub.org/public/igem/BBa_K091117/annotation1964074/range1964074/1
type: Range
persistent identity: range1964074
display id: range1964074
version: 1
direction: direction/forward
top level: BBa_K091117
start: 113
end: 118
orientation: inline

BBa_K180003
type: ComponentDefinition
persistent identity: BBa K180003
display id: BBa_K180003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PTAC testing plasmid (GFP) - basic
creator: Richard Wilson, Stephanie Chambers
created: 2009-10-15T11:00:00Z
modified: 2015-05-08T01:11:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _278_
m user id: 0
owner id: 4082
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: As per BBa_K180002, omitting the RBS and STOP biobrick parts (B0034 and B0015) for speed and ease of construction.
mutable notes: Assess if the RBS (B0034) sequence is needed prior to GFP (E0040) whilst still able to test function of part K180000.
mutable provenance: See parts K180000, E0040
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_E0040
component: BBa_K180000
sequence annotation: BBa_K180000
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_K180003_sequence/1

BBa_E0040
type: Component
persistent identity: component2044074
display id: component2044074
version: 1
top level: BBa_K180003
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_K180000
type: Component
persistent identity: component2044072
display id: component2044072
version: 1
top level: BBa_K180003
access: public
definition: BBa_K180000

BBa_K180000
type: SequenceAnnotation
persistent identity: annotation2044072
display id: annotation2044072
version: 1
top level: BBa_K180003
location: https://synbiohub.org/public/igem/BBa_K180003/annotation2044072/range2044072/1
component: BBa_K180000

https://synbiohub.org/public/igem/BBa_K180003/annotation2044072/range2044072/1
type: Range
persistent identity: range2044072
display id: range2044072
version: 1
direction: direction/reverse
top level: BBa_K180003
start: 1
end: 1447
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2044074
display id: annotation2044074
version: 1
top level: BBa_K180003
location: https://synbiohub.org/public/igem/BBa_K180003/annotation2044074/range2044074/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K180003/annotation2044074/range2044074/1
type: Range
persistent identity: range2044074
display id: range2044074
version: 1
direction: direction/reverse
top level: BBa_K180003
start: 1454
end: 2173
orientation: inline

BBa_I741021
type: ComponentDefinition
persistent identity: BBa I741021
display id: BBa_I741021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: promoter to xylA without CRP and several binding sites for xylR
creator: Lucien Weiss
created: 2007-06-20T11:00:00Z
modified: 2015-08-31T04:08:02Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _143_
m user id: 0
owner id: 1763
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: removed crp binding site removed rbs removed several xylR binding sites
mutable provenance: ecoli K12 genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: promoter
sequence: https://synbiohub.org/public/igem/BBa_I741021_sequence/1

promoter
type: SequenceAnnotation
persistent identity: annotation1935206
display id: annotation1935206
version: 1
top level: BBa_I741021
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I741021/annotation1935206/range1935206/1

https://synbiohub.org/public/igem/BBa_I741021/annotation1935206/range1935206/1
type: Range
persistent identity: range1935206
display id: range1935206
version: 1
direction: direction/reverse
top level: BBa_I741021
start: 15
end: 75
orientation: inline

lux pR
type: ComponentDefinition
persistent identity: BBa R1062
display id: BBa_R1062
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter, Standard (luxR and HSL regulated -- lux pR)
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr Drew Endy
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: [Note: This is the same part as R0062 except that the -10 and -35 sites and spacing have been changed to comply with BBa_S0001]. Promoter activated by LuxR in concert with HSL The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the LuxR activator protein complexed with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
mutable notes: This promoter is based on the Vibrio fischeri quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below. <img src="<bb_file part=BBa_R0062>Image1.gif</bb_file>" width="614" height="362"> Modified to comply with BBa_S0001: TTGACA-17N-GATACT
mutable provenance: V. fischeri
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: LuxR/HSL
sequence annotation: BBa_R1062
sequence annotation: -35
sequence annotation: start
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R1062_sequence/1

LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2098
display id: annotation2098
version: 1
top level: lux pR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R1062/annotation2098/range2098/1

https://synbiohub.org/public/igem/BBa_R1062/annotation2098/range2098/1
type: Range
persistent identity: range2098
display id: range2098
version: 1
direction: direction/forward
top level: lux pR
start: 1
end: 20
orientation: inline

BBa_R1062
type: SequenceAnnotation
persistent identity: annotation7077
display id: annotation7077
version: 1
top level: lux pR
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R1062/annotation7077/range7077/1

https://synbiohub.org/public/igem/BBa_R1062/annotation7077/range7077/1
type: Range
persistent identity: range7077
display id: range7077
version: 1
direction: direction/forward
top level: lux pR
start: 1
end: 56
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2101
display id: annotation2101
version: 1
top level: lux pR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1062/annotation2101/range2101/1

https://synbiohub.org/public/igem/BBa_R1062/annotation2101/range2101/1
type: Range
persistent identity: range2101
display id: range2101
version: 1
direction: direction/forward
top level: lux pR
start: 20
end: 25
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2100
display id: annotation2100
version: 1
top level: lux pR
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_R1062/annotation2100/range2100/1

https://synbiohub.org/public/igem/BBa_R1062/annotation2100/range2100/1
type: Range
persistent identity: range2100
display id: range2100
version: 1
direction: direction/forward
top level: lux pR
start: 54
end: 54
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2099
display id: annotation2099
version: 1
top level: lux pR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1062/annotation2099/range2099/1

https://synbiohub.org/public/igem/BBa_R1062/annotation2099/range2099/1
type: Range
persistent identity: range2099
display id: range2099
version: 1
direction: direction/forward
top level: lux pR
start: 42
end: 48
orientation: inline

pMalT
type: ComponentDefinition
persistent identity: BBa J03007
display id: BBa_J03007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Maltose specific promotor
creator: James Godman
created: 2005-11-03T12:00:00Z
modified: 2015-08-31T04:08:13Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _15_
m user id: 0
owner id: 405
owning group id: 15
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Binds Maltose and stimulates transcription
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J03007_sequence/1

BBa_J05215
type: ComponentDefinition
persistent identity: BBa J05215
display id: BBa_J05215
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Regulator for R1-CREBH
creator: Alexander Roth
created: 2005-09-07T11:00:00Z
modified: 2015-08-31T04:08:15Z
discontinued: false
dominant: false
group u list: _34_
m user id: 0
owner id: 412
owning group id: 34
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: binding sites for R3-ATF6 and R2-YAP7
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J05215_sequence/1

T7 auto-am
type: ComponentDefinition
persistent identity: BBa K606036
display id: BBa_K606036
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pT7 SpoVG T7 SpoVG GFP T7terminator.
creator: Axel S??guret
created: 2011-09-17T11:00:00Z
modified: 2015-05-08T01:12:51Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _778_
m user id: 0
owner id: 8931
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: T7 and GFP expression system due to T7 polymerase
mutable notes: biobrick assembly
mutable provenance: B. subtilis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K145001
component: BBa_K143021
component: BBa_K606029
component: BBa_I719005
sequence annotation: BBa_I719005
sequence annotation: BBa_K145001
sequence annotation: BBa_K606029
sequence annotation: BBa_K143021
sequence: https://synbiohub.org/public/igem/BBa_K606036_sequence/1

BBa_K145001
type: Component
persistent identity: component2224487
display id: component2224487
version: 1
top level: T7 auto-am
access: public
definition: https://synbiohub.org/public/igem/BBa_K145001/1

BBa_K143021
type: Component
persistent identity: component2224482
display id: component2224482
version: 1
top level: T7 auto-am
access: public
definition: https://synbiohub.org/public/igem/BBa_K143021/1

BBa_K606029
type: Component
persistent identity: component2224502
display id: component2224502
version: 1
top level: T7 auto-am
access: public
definition: https://synbiohub.org/public/igem/BBa_K606029/1

BBa_I719005
type: Component
persistent identity: component2224480
display id: component2224480
version: 1
top level: T7 auto-am
access: public
definition: https://synbiohub.org/public/igem/BBa_I719005/1

BBa_I719005
type: SequenceAnnotation
persistent identity: annotation2224480
display id: annotation2224480
version: 1
top level: T7 auto-am
location: https://synbiohub.org/public/igem/BBa_K606036/annotation2224480/range2224480/1
component: BBa_I719005

https://synbiohub.org/public/igem/BBa_K606036/annotation2224480/range2224480/1
type: Range
persistent identity: range2224480
display id: range2224480
version: 1
direction: direction/reverse
top level: T7 auto-am
start: 1
end: 23
orientation: inline

BBa_K145001
type: SequenceAnnotation
persistent identity: annotation2224487
display id: annotation2224487
version: 1
top level: T7 auto-am
location: https://synbiohub.org/public/igem/BBa_K606036/annotation2224487/range2224487/1
component: BBa_K145001

https://synbiohub.org/public/igem/BBa_K606036/annotation2224487/range2224487/1
type: Range
persistent identity: range2224487
display id: range2224487
version: 1
direction: direction/reverse
top level: T7 auto-am
start: 50
end: 2704
orientation: inline

BBa_K606029
type: SequenceAnnotation
persistent identity: annotation2224502
display id: annotation2224502
version: 1
top level: T7 auto-am
location: https://synbiohub.org/public/igem/BBa_K606036/annotation2224502/range2224502/1
component: BBa_K606029

https://synbiohub.org/public/igem/BBa_K606036/annotation2224502/range2224502/1
type: Range
persistent identity: range2224502
display id: range2224502
version: 1
direction: direction/reverse
top level: T7 auto-am
start: 2713
end: 3748
orientation: inline

BBa_K143021
type: SequenceAnnotation
persistent identity: annotation2224482
display id: annotation2224482
version: 1
top level: T7 auto-am
location: https://synbiohub.org/public/igem/BBa_K606036/annotation2224482/range2224482/1
component: BBa_K143021

https://synbiohub.org/public/igem/BBa_K606036/annotation2224482/range2224482/1
type: Range
persistent identity: range2224482
display id: range2224482
version: 1
direction: direction/reverse
top level: T7 auto-am
start: 32
end: 43
orientation: inline

BBa_I765007
type: ComponentDefinition
persistent identity: BBa I765007
display id: BBa_I765007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fe and UV promoters
creator: Claudia Guevara and Alex Quintero
created: 2007-10-25T11:00:00Z
modified: 2015-08-31T04:08:09Z
discontinued: false
dominant: false
experience: experience/None
group u list: _118_
m user id: 0
owner id: 2267
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Regulated by the presence of Iron and UV light irradiation
mutable notes: None
mutable provenance: Genomic sequence
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_I765000
component: BBa_I765001
sequence annotation: BBa_I765000
sequence annotation: BBa_I765001
sequence: https://synbiohub.org/public/igem/BBa_I765007_sequence/1

BBa_I765000
type: Component
persistent identity: component1958274
display id: component1958274
version: 1
top level: BBa_I765007
access: public
definition: https://synbiohub.org/public/igem/BBa_I765000/1

BBa_I765001
type: Component
persistent identity: component1958276
display id: component1958276
version: 1
top level: BBa_I765007
access: public
definition: pUV

BBa_I765000
type: SequenceAnnotation
persistent identity: annotation1958274
display id: annotation1958274
version: 1
top level: BBa_I765007
location: https://synbiohub.org/public/igem/BBa_I765007/annotation1958274/range1958274/1
component: BBa_I765000

https://synbiohub.org/public/igem/BBa_I765007/annotation1958274/range1958274/1
type: Range
persistent identity: range1958274
display id: range1958274
version: 1
direction: direction/reverse
top level: BBa_I765007
start: 1
end: 1044
orientation: inline

BBa_I765001
type: SequenceAnnotation
persistent identity: annotation1958276
display id: annotation1958276
version: 1
top level: BBa_I765007
location: https://synbiohub.org/public/igem/BBa_I765007/annotation1958276/range1958276/1
component: BBa_I765001

https://synbiohub.org/public/igem/BBa_I765007/annotation1958276/range1958276/1
type: Range
persistent identity: range1958276
display id: range1958276
version: 1
direction: direction/reverse
top level: BBa_I765007
start: 1053
end: 1128
orientation: inline

BBa_K180006
type: ComponentDefinition
persistent identity: BBa K180006
display id: BBa_K180006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Game of Life - Primary plasmid (part 2) [lux pR, GFP and LacI generator]
creator: Richard Wilson, Stephanie Chambers
created: 2009-10-15T11:00:00Z
modified: 2015-05-08T01:11:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _278_
m user id: 0
owner id: 4082
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Part of the iGEM2009 Team:Southampton Game of Life primary plasmid.
mutable notes: Regulated expression of LacI (C0012) and GFP (E0040) by PLUX promoter (R0062), controlled by presence/absence of AHL-LuxR.
mutable provenance: See relevant basic biobrick parts (R0062, C0012, B0034, K180001, B0015)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0062
component: BBa_B0015
component: BBa_C0012
component: BBa_K180001
component: BBa_B0034
sequence annotation: BBa_B0015
sequence annotation: BBa_K180001
sequence annotation: BBa_B0034
sequence annotation: BBa_C0012
sequence annotation: BBa_R0062
sequence: https://synbiohub.org/public/igem/BBa_K180006_sequence/1

BBa_R0062
type: Component
persistent identity: component2267822
display id: component2267822
version: 1
top level: BBa_K180006
access: public
definition: lux pR

BBa_B0015
type: Component
persistent identity: component2267845
display id: component2267845
version: 1
top level: BBa_K180006
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_C0012
type: Component
persistent identity: component2267830
display id: component2267830
version: 1
top level: BBa_K180006
access: public
definition: https://synbiohub.org/public/igem/BBa_C0012/1

BBa_K180001
type: Component
persistent identity: component2267838
display id: component2267838
version: 1
top level: BBa_K180006
access: public
definition: https://synbiohub.org/public/igem/BBa_K180001/1

BBa_B0034
type: Component
persistent identity: component2267834
display id: component2267834
version: 1
top level: BBa_K180006
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: Component
persistent identity: component2267828
display id: component2267828
version: 1
top level: BBa_K180006
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2267845
display id: annotation2267845
version: 1
top level: BBa_K180006
location: https://synbiohub.org/public/igem/BBa_K180006/annotation2267845/range2267845/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K180006/annotation2267845/range2267845/1
type: Range
persistent identity: range2267845
display id: range2267845
version: 1
direction: direction/reverse
top level: BBa_K180006
start: 2023
end: 2151
orientation: inline

BBa_K180001
type: SequenceAnnotation
persistent identity: annotation2267838
display id: annotation2267838
version: 1
top level: BBa_K180006
location: https://synbiohub.org/public/igem/BBa_K180006/annotation2267838/range2267838/1
component: BBa_K180001

https://synbiohub.org/public/igem/BBa_K180006/annotation2267838/range2267838/1
type: Range
persistent identity: range2267838
display id: range2267838
version: 1
direction: direction/reverse
top level: BBa_K180006
start: 1261
end: 2014
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2267834
display id: annotation2267834
version: 1
top level: BBa_K180006
location: https://synbiohub.org/public/igem/BBa_K180006/annotation2267834/range2267834/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K180006/annotation2267834/range2267834/1
type: Range
persistent identity: range2267834
display id: range2267834
version: 1
direction: direction/reverse
top level: BBa_K180006
start: 1243
end: 1254
orientation: inline

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation2267830
display id: annotation2267830
version: 1
top level: BBa_K180006
location: https://synbiohub.org/public/igem/BBa_K180006/annotation2267830/range2267830/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_K180006/annotation2267830/range2267830/1
type: Range
persistent identity: range2267830
display id: range2267830
version: 1
direction: direction/forward
top level: BBa_K180006
start: 82
end: 1209
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2267822
display id: annotation2267822
version: 1
top level: BBa_K180006
location: https://synbiohub.org/public/igem/BBa_K180006/annotation2267822/range2267822/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K180006/annotation2267822/range2267822/1
type: Range
persistent identity: range2267822
display id: range2267822
version: 1
direction: direction/forward
top level: BBa_K180006
start: 1
end: 55
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2267828
display id: annotation2267828
version: 1
top level: BBa_K180006
location: https://synbiohub.org/public/igem/BBa_K180006/annotation2267828/range2267828/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K180006/annotation2267828/range2267828/1
type: Range
persistent identity: range2267828
display id: range2267828
version: 1
direction: direction/reverse
top level: BBa_K180006
start: 64
end: 75
orientation: inline

GAL1
type: ComponentDefinition
persistent identity: BBa J63006
display id: BBa_J63006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: yeast GAL1 promoter
creator: Caroline Ajo-Franklin
created: 2006-10-10T11:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _97_
m user id: 0
owner id: 545
owning group id: 97
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: GAL1 promoter from S. cerevisiae
mutable notes: unchanged from genomic sequence
mutable provenance: genomic GAL1 locus
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J63006_sequence/1

BBa_I12036
type: ComponentDefinition
persistent identity: BBa I12036
display id: BBa_I12036
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Modified lamdba Prm promoter (cooperative repression by 434 cI)
creator: Hans
created: 2004-08-17T11:00:00Z
modified: 2015-08-31T04:07:31Z
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 103
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Lamdba Prm promoter modified to be activated by lamda repressor (cI) and repressed by 434 repressor (cI). Two operators are used for both activation and repression to enhance cooperativity.
mutable notes: The O-R3 site of lambda (17 nt) was replaced by the O-R1 site of 434 (14 nt) to facilitate repression by 434 cI. The new 434 O-R1 is 5'-justified with the lambda O-R3 it replaced, leaving the -10 sequence intact. The 434 O-R2 site replaces the wildtype labmda promoter starting at -5, thereby preserving the 8 nt between 434 O-R1 and O-R2 in the wildtype. The mRNA transcript will contain an additional 9 nt compared to wildtype lambda phage.
mutable provenance: Bushman, F. D. The bacteriophage 434 right operator roles of O-R1, O-R2, and O-R3. J. Mol. Biol. (1993) 230, 28-40.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: start
sequence annotation: OR1 lambda
sequence annotation: OR2 434
sequence annotation: -10
sequence annotation: OR2 lambda
sequence annotation: OR1 434
sequence: https://synbiohub.org/public/igem/BBa_I12036_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation1056739
display id: annotation1056739
version: 1
top level: BBa_I12036
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I12036/annotation1056739/range1056739/1

https://synbiohub.org/public/igem/BBa_I12036/annotation1056739/range1056739/1
type: Range
persistent identity: range1056739
display id: range1056739
version: 1
direction: direction/forward
top level: BBa_I12036
start: 48
end: 53
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1062203
display id: annotation1062203
version: 1
top level: BBa_I12036
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I12036/annotation1062203/range1062203/1

https://synbiohub.org/public/igem/BBa_I12036/annotation1062203/range1062203/1
type: Range
persistent identity: range1062203
display id: range1062203
version: 1
direction: direction/forward
top level: BBa_I12036
start: 83
end: 83
orientation: inline

OR1 lambda
type: SequenceAnnotation
persistent identity: annotation1056735
display id: annotation1056735
version: 1
top level: BBa_I12036
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12036/annotation1056735/range1056735/1

https://synbiohub.org/public/igem/BBa_I12036/annotation1056735/range1056735/1
type: Range
persistent identity: range1056735
display id: range1056735
version: 1
direction: direction/forward
top level: BBa_I12036
start: 9
end: 25
orientation: inline

OR2 434
type: SequenceAnnotation
persistent identity: annotation1056738
display id: annotation1056738
version: 1
top level: BBa_I12036
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12036/annotation1056738/range1056738/1

https://synbiohub.org/public/igem/BBa_I12036/annotation1056738/range1056738/1
type: Range
persistent identity: range1056738
display id: range1056738
version: 1
direction: direction/forward
top level: BBa_I12036
start: 78
end: 91
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1056740
display id: annotation1056740
version: 1
top level: BBa_I12036
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I12036/annotation1056740/range1056740/1

https://synbiohub.org/public/igem/BBa_I12036/annotation1056740/range1056740/1
type: Range
persistent identity: range1056740
display id: range1056740
version: 1
direction: direction/forward
top level: BBa_I12036
start: 71
end: 76
orientation: inline

OR2 lambda
type: SequenceAnnotation
persistent identity: annotation1056736
display id: annotation1056736
version: 1
top level: BBa_I12036
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12036/annotation1056736/range1056736/1

https://synbiohub.org/public/igem/BBa_I12036/annotation1056736/range1056736/1
type: Range
persistent identity: range1056736
display id: range1056736
version: 1
direction: direction/forward
top level: BBa_I12036
start: 33
end: 49
orientation: inline

OR1 434
type: SequenceAnnotation
persistent identity: annotation1056737
display id: annotation1056737
version: 1
top level: BBa_I12036
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12036/annotation1056737/range1056737/1

https://synbiohub.org/public/igem/BBa_I12036/annotation1056737/range1056737/1
type: Range
persistent identity: range1056737
display id: range1056737
version: 1
direction: direction/forward
top level: BBa_I12036
start: 56
end: 69
orientation: inline

BBa_K1709004
type: ComponentDefinition
persistent identity: BBa K1709004
display id: BBa_K1709004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LuxR-E
creator: Astrid Deryckere
created: 2015-09-17T11:00:00Z
modified: 2015-09-18T11:21:02Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2129_
m user id: 25389
owner id: 25389
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Encodes a transcription activator for the right handed Lux promotor. Two molecules of acyl-homoserine lactones (AHL) form a complex with two molecules of the LuxR protein. This complex then binds to a palyndromic site on the lux promoter, increasing its transcription rate. LuxI, LuxR and the right lux promoter are all derived from V. fischeri.
mutable notes: Biobrick BBa_C0061 still contained a Barcode sequence which was deleted. For our own use, 9 bp were exchanged to create unique restriction sites and to optimize DNA folding.
mutable provenance: Sequence is originating from V. fischeri. Here, the sequence from BBa_I0462 was used and optimized.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: LuxR
sequence annotation: ATG Start Codon
sequence annotation: Ochre Stop Codon
sequence annotation: E-tag
sequence: https://synbiohub.org/public/igem/BBa_K1709004_sequence/1

LuxR
type: SequenceAnnotation
persistent identity: annotation2470868
display id: annotation2470868
version: 1
top level: BBa_K1709004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1709004/annotation2470868/range2470868/1

https://synbiohub.org/public/igem/BBa_K1709004/annotation2470868/range2470868/1
type: Range
persistent identity: range2470868
display id: range2470868
version: 1
direction: direction/forward
top level: BBa_K1709004
start: 1
end: 750
orientation: inline

ATG Start Codon
type: SequenceAnnotation
persistent identity: annotation2470847
display id: annotation2470847
version: 1
top level: BBa_K1709004
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1709004/annotation2470847/range2470847/1

https://synbiohub.org/public/igem/BBa_K1709004/annotation2470847/range2470847/1
type: Range
persistent identity: range2470847
display id: range2470847
version: 1
direction: direction/forward
top level: BBa_K1709004
start: 1
end: 3
orientation: inline

Ochre Stop Codon
type: SequenceAnnotation
persistent identity: annotation2470849
display id: annotation2470849
version: 1
top level: BBa_K1709004
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1709004/annotation2470849/range2470849/1

https://synbiohub.org/public/igem/BBa_K1709004/annotation2470849/range2470849/1
type: Range
persistent identity: range2470849
display id: range2470849
version: 1
direction: direction/forward
top level: BBa_K1709004
start: 799
end: 801
orientation: inline

E-tag
type: SequenceAnnotation
persistent identity: annotation2470866
display id: annotation2470866
version: 1
top level: BBa_K1709004
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1709004/annotation2470866/range2470866/1

https://synbiohub.org/public/igem/BBa_K1709004/annotation2470866/range2470866/1
type: Range
persistent identity: range2470866
display id: range2470866
version: 1
direction: direction/forward
top level: BBa_K1709004
start: 753
end: 801
orientation: inline

BBa_K566005
type: ComponentDefinition
persistent identity: BBa K566005
display id: BBa_K566005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCpcG2 promoter (Green light inducible)
creator: Daniel Rodriguez
created: 2011-09-25T11:00:00Z
modified: 2015-05-08T01:12:43Z
discontinued: false
dominant: false
experience: experience/None
group u list: _734_
m user id: 0
owner id: 8650
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: pCpcG2 promoter from pJT122 plasmid of Dr. Jeff Tabor. This promoter is inducible with green light through ccaR and ccaS photoreceptor system.
mutable notes: Search for illegal sites was performed, no sites were found.
mutable provenance: Sequence was obtained from pJT122 plasmid of Dr. Jeff Tabor, but it was synthesized.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: pCpcG2
sequence: https://synbiohub.org/public/igem/BBa_K566005_sequence/1

pCpcG2
type: SequenceAnnotation
persistent identity: annotation2145892
display id: annotation2145892
version: 1
top level: BBa_K566005
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K566005/annotation2145892/range2145892/1

https://synbiohub.org/public/igem/BBa_K566005/annotation2145892/range2145892/1
type: Range
persistent identity: range2145892
display id: range2145892
version: 1
direction: direction/forward
top level: BBa_K566005
start: 1
end: 240
orientation: inline

BBa_K112701
type: ComponentDefinition
persistent identity: BBa K112701
display id: BBa_K112701
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: hns promoter
creator: Bing Xia
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:18Z
discontinued: false
dominant: false
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Phns in BBb1 format. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable provenance: Construction of [<Phns>] Basic Part K112701 PCR dv001/dv006 on Phns from E. coli K12 MG1655 (~342 bp, gp = A) PCR dv005/dv003 on Phns from E. coli K12 MG1655 (225 bp, gp = B) PCR dv004/dv002 on Phns from E. coli K12 MG1655 (~149 bp, gp = C) --------------------------------------------------- PCR dv001/dv003 on A+B (355 bp, gp = D) --------------------------------------------------- PCR dv001/dv002 on C+D (697bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+697) Product is pBca1256-K112701 ----------------------------------------------- dv001 Forward Biobricking of [<Phns>] CgATAgaattcATGagatctGAAATATAGCTGTGCCATCAGCC dv002 Reverse Biobricking of [<Phns>] cagtcggatccGCACGAAGAGTACGGATG dv003 Removing the 2nd EcoRI site in [<Phns>] (R) GCCAGGAATGTAAGGgATTCAAAATTGTTC dv004 Removing the 2ND EcoRI site in [<Phns>] (F) GAACAATTTTGAATcCCTTACATTCCTGGC dv005 Removing the FIRST EcoRI site in [<Phns>] (F)CGCTTAATAGGGgATTCTCGTAAACAC dv006 Removing the FIRST EcoRI site in [<Phns>] (R)GTGTTTACGAGAATcCCCTATTAAGCG
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K112701_sequence/1

BBa_K1946006
type: ComponentDefinition
persistent identity: BBa K1946006
display id: BBa_K1946006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pu
creator: Ayşe Naz Ozant??rk
created: 2016-10-12T11:00:00Z
modified: 2016-10-13T06:06:13Z
discontinued: false
dominant: false
group u list: _2413_
m user id: 29837
owner id: 29837
owning group id: 9
status: status/Planning
bookmark: false
mutable description: pu promoter drives transcription of the upper operon of Pseudomonas putida mt-2 TOL plasmid for degradation of toluene and xylenes. pu is activated by the activator protein XylR (BBa_K1946005) in the presence of toluene and xylenes.
mutable notes: .
mutable provenance: Synthetically synthesized from GeneWiz based on Pseudomonas putida TOL plasmid
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: pu promoter
sequence: https://synbiohub.org/public/igem/BBa_K1946006_sequence/1

pu promoter
type: SequenceAnnotation
persistent identity: annotation2497342
display id: annotation2497342
version: 1
top level: BBa_K1946006
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1946006/annotation2497342/range2497342/1

https://synbiohub.org/public/igem/BBa_K1946006/annotation2497342/range2497342/1
type: Range
persistent identity: range2497342
display id: range2497342
version: 1
direction: direction/forward
top level: BBa_K1946006
start: 1
end: 322
orientation: inline

Pu
type: ComponentDefinition
persistent identity: BBa I723020
display id: BBa_I723020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pu
creator: Scott Ramsay
created: 2007-10-24T11:00:00Z
modified: 2015-08-31T04:07:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _126_
m user id: 0
owner id: 2140
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is the promoter activated by the transcriptional regulator XylR (BBa_I723136) in response to detection of environmental xylene.
mutable notes: [Christine/Maia]
mutable provenance: [Christine/Maia]
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Pu
sequence: https://synbiohub.org/public/igem/BBa_I723020_sequence/1

Pu
type: SequenceAnnotation
persistent identity: annotation1955192
display id: annotation1955192
version: 1
top level: Pu
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I723020/annotation1955192/range1955192/1

https://synbiohub.org/public/igem/BBa_I723020/annotation1955192/range1955192/1
type: Range
persistent identity: range1955192
display id: range1955192
version: 1
direction: direction/forward
top level: Pu
start: 1
end: 320
orientation: inline

BBa_J64712
type: ComponentDefinition
persistent identity: BBa J64712
display id: BBa_J64712
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LasR/LasI Inducible & RHLR/RHLI repressible Promoter
creator: laura lavery
created: 2007-03-24T12:00:00Z
modified: 2015-05-08T01:08:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 1431
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is similar to BBa_R0079 but has an added binding site for RHLR/RHLI in between the -35 and -10 region. Thus this promoter is induced by LasR/LasI but repressed by RHLR/RHLI binding.
mutable notes: The natural spacer between the -35 and -10 is 14bp and the natural O1 site for RHLR/RHLI is 20bp long. There was not much over lap of the -35 or the -10 with this sequence, so it was nessicary to chop off some of the binding site sequence in order to maintain the correct spacing and preserve the -35 and -10 sequence. The binding site sequence is missing a tcc on is 5' end and a t on its 3' end.
mutable provenance: Rust L. et. al., "Analysis of the Pseudomonas aeruginosa Elastase (lasB) Regulatory Region". Journal of Bacteriology, 1996.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: -35
sequence annotation: LasR/LasI O2
sequence annotation: LasR/LasI O1
sequence annotation: RHLR/RHLI
sequence: https://synbiohub.org/public/igem/BBa_J64712_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation1921068
display id: annotation1921068
version: 1
top level: BBa_J64712
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J64712/annotation1921068/range1921068/1

https://synbiohub.org/public/igem/BBa_J64712/annotation1921068/range1921068/1
type: Range
persistent identity: range1921068
display id: range1921068
version: 1
direction: direction/forward
top level: BBa_J64712
start: 140
end: 145
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1921067
display id: annotation1921067
version: 1
top level: BBa_J64712
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J64712/annotation1921067/range1921067/1

https://synbiohub.org/public/igem/BBa_J64712/annotation1921067/range1921067/1
type: Range
persistent identity: range1921067
display id: range1921067
version: 1
direction: direction/forward
top level: BBa_J64712
start: 117
end: 122
orientation: inline

LasR/LasI O2
type: SequenceAnnotation
persistent identity: annotation1921064
display id: annotation1921064
version: 1
top level: BBa_J64712
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_J64712/annotation1921064/range1921064/1

https://synbiohub.org/public/igem/BBa_J64712/annotation1921064/range1921064/1
type: Range
persistent identity: range1921064
display id: range1921064
version: 1
direction: direction/forward
top level: BBa_J64712
start: 46
end: 64
orientation: inline

LasR/LasI O1
type: SequenceAnnotation
persistent identity: annotation1921065
display id: annotation1921065
version: 1
top level: BBa_J64712
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_J64712/annotation1921065/range1921065/1

https://synbiohub.org/public/igem/BBa_J64712/annotation1921065/range1921065/1
type: Range
persistent identity: range1921065
display id: range1921065
version: 1
direction: direction/forward
top level: BBa_J64712
start: 106
end: 125
orientation: inline

RHLR/RHLI
type: SequenceAnnotation
persistent identity: annotation1921066
display id: annotation1921066
version: 1
top level: BBa_J64712
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J64712/annotation1921066/range1921066/1

https://synbiohub.org/public/igem/BBa_J64712/annotation1921066/range1921066/1
type: Range
persistent identity: range1921066
display id: range1921066
version: 1
direction: direction/forward
top level: BBa_J64712
start: 125
end: 141
orientation: inline

BBa_K1067007
type: ComponentDefinition
persistent identity: BBa K1067007
display id: BBa_K1067007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tight pBAD with araC
creator: Kristian Davidsen and Helen Cook
created: 2013-09-27T11:00:00Z
modified: 2015-05-08T01:09:00Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1376_
m user id: 0
owner id: 16028
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: ggg
mutable notes: ggg
mutable provenance: ggg
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: araI1
sequence annotation: -35
sequence annotation: araO2
sequence annotation: araO1L
sequence annotation: -10
sequence annotation: araO1R
sequence annotation: araC
sequence annotation: araI2
sequence annotation: Tight pBAD promoter
sequence annotation: CRP binding site
sequence: https://synbiohub.org/public/igem/BBa_K1067007_sequence/1

araI1
type: SequenceAnnotation
persistent identity: annotation2366484
display id: annotation2366484
version: 1
top level: BBa_K1067007
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1067007/annotation2366484/range2366484/1

https://synbiohub.org/public/igem/BBa_K1067007/annotation2366484/range2366484/1
type: Range
persistent identity: range2366484
display id: range2366484
version: 1
direction: direction/forward
top level: BBa_K1067007
start: 1119
end: 1135
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2366487
display id: annotation2366487
version: 1
top level: BBa_K1067007
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1067007/annotation2366487/range2366487/1

https://synbiohub.org/public/igem/BBa_K1067007/annotation2366487/range2366487/1
type: Range
persistent identity: range2366487
display id: range2366487
version: 1
direction: direction/forward
top level: BBa_K1067007
start: 1153
end: 1158
orientation: inline

araO2
type: SequenceAnnotation
persistent identity: annotation2366480
display id: annotation2366480
version: 1
top level: BBa_K1067007
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1067007/annotation2366480/range2366480/1

https://synbiohub.org/public/igem/BBa_K1067007/annotation2366480/range2366480/1
type: Range
persistent identity: range2366480
display id: range2366480
version: 1
direction: direction/forward
top level: BBa_K1067007
start: 908
end: 924
orientation: inline

araO1L
type: SequenceAnnotation
persistent identity: annotation2366481
display id: annotation2366481
version: 1
top level: BBa_K1067007
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1067007/annotation2366481/range2366481/1

https://synbiohub.org/public/igem/BBa_K1067007/annotation2366481/range2366481/1
type: Range
persistent identity: range2366481
display id: range2366481
version: 1
direction: direction/forward
top level: BBa_K1067007
start: 1045
end: 1061
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2366488
display id: annotation2366488
version: 1
top level: BBa_K1067007
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1067007/annotation2366488/range2366488/1

https://synbiohub.org/public/igem/BBa_K1067007/annotation2366488/range2366488/1
type: Range
persistent identity: range2366488
display id: range2366488
version: 1
direction: direction/forward
top level: BBa_K1067007
start: 1177
end: 1182
orientation: inline

araO1R
type: SequenceAnnotation
persistent identity: annotation2366482
display id: annotation2366482
version: 1
top level: BBa_K1067007
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1067007/annotation2366482/range2366482/1

https://synbiohub.org/public/igem/BBa_K1067007/annotation2366482/range2366482/1
type: Range
persistent identity: range2366482
display id: range2366482
version: 1
direction: direction/forward
top level: BBa_K1067007
start: 1066
end: 1082
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation2366479
display id: annotation2366479
version: 1
top level: BBa_K1067007
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1067007/annotation2366479/range2366479/1

https://synbiohub.org/public/igem/BBa_K1067007/annotation2366479/range2366479/1
type: Range
persistent identity: range2366479
display id: range2366479
version: 1
direction: direction/forward
top level: BBa_K1067007
start: 1
end: 880
orientation: inline

araI2
type: SequenceAnnotation
persistent identity: annotation2366485
display id: annotation2366485
version: 1
top level: BBa_K1067007
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1067007/annotation2366485/range2366485/1

https://synbiohub.org/public/igem/BBa_K1067007/annotation2366485/range2366485/1
type: Range
persistent identity: range2366485
display id: range2366485
version: 1
direction: direction/forward
top level: BBa_K1067007
start: 1140
end: 1157
orientation: inline

Tight pBAD promoter
type: SequenceAnnotation
persistent identity: annotation2366486
display id: annotation2366486
version: 1
top level: BBa_K1067007
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1067007/annotation2366486/range2366486/1

https://synbiohub.org/public/igem/BBa_K1067007/annotation2366486/range2366486/1
type: Range
persistent identity: range2366486
display id: range2366486
version: 1
direction: direction/forward
top level: BBa_K1067007
start: 1153
end: 1189
orientation: inline

CRP binding site
type: SequenceAnnotation
persistent identity: annotation2366483
display id: annotation2366483
version: 1
top level: BBa_K1067007
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1067007/annotation2366483/range2366483/1

https://synbiohub.org/public/igem/BBa_K1067007/annotation2366483/range2366483/1
type: Range
persistent identity: range2366483
display id: range2366483
version: 1
direction: direction/forward
top level: BBa_K1067007
start: 1087
end: 1108
orientation: inline

AraC
type: ComponentDefinition
persistent identity: BBa R0080
display id: BBa_R0080
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (AraC regulated)
creator: Sara Neves (Fighting Darwins)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: AraC operator, truncated to include araO1, araI1, araI2, c-amp1, and c-amp2 sites. This operator should *activate* transcription in the presence of AraC; b/c the operator lacks the araO2 site, there should not be araC-mediated repression.
mutable provenance: GenBank: J01641 (www.ncbi.nlm.nih.gov)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: araO1
sequence annotation: c-amp1
sequence annotation: c-amp2
sequence annotation: -35
sequence annotation: ara1 and ara2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0080_sequence/1

araO1
type: SequenceAnnotation
persistent identity: annotation301457
display id: annotation301457
version: 1
top level: AraC
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_R0080/annotation301457/range301457/1

https://synbiohub.org/public/igem/BBa_R0080/annotation301457/range301457/1
type: Range
persistent identity: range301457
display id: range301457
version: 1
direction: direction/forward
top level: AraC
start: 6
end: 44
orientation: inline

c-amp1
type: SequenceAnnotation
persistent identity: annotation301458
display id: annotation301458
version: 1
top level: AraC
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0080/annotation301458/range301458/1

https://synbiohub.org/public/igem/BBa_R0080/annotation301458/range301458/1
type: Range
persistent identity: range301458
display id: range301458
version: 1
direction: direction/forward
top level: AraC
start: 43
end: 72
orientation: inline

c-amp2
type: SequenceAnnotation
persistent identity: annotation301456
display id: annotation301456
version: 1
top level: AraC
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0080/annotation301456/range301456/1

https://synbiohub.org/public/igem/BBa_R0080/annotation301456/range301456/1
type: Range
persistent identity: range301456
display id: range301456
version: 1
direction: direction/forward
top level: AraC
start: 4
end: 29
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation308601
display id: annotation308601
version: 1
top level: AraC
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0080/annotation308601/range308601/1

https://synbiohub.org/public/igem/BBa_R0080/annotation308601/range308601/1
type: Range
persistent identity: range308601
display id: range308601
version: 1
direction: direction/forward
top level: AraC
start: 113
end: 118
orientation: inline

ara1 and ara2
type: SequenceAnnotation
persistent identity: annotation301462
display id: annotation301462
version: 1
top level: AraC
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0080/annotation301462/range301462/1

https://synbiohub.org/public/igem/BBa_R0080/annotation301462/range301462/1
type: Range
persistent identity: range301462
display id: range301462
version: 1
direction: direction/forward
top level: AraC
start: 73
end: 101
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation308602
display id: annotation308602
version: 1
top level: AraC
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0080/annotation308602/range308602/1

https://synbiohub.org/public/igem/BBa_R0080/annotation308602/range308602/1
type: Range
persistent identity: range308602
display id: range308602
version: 1
direction: direction/forward
top level: AraC
start: 136
end: 141
orientation: inline

BBa_K180020
type: ComponentDefinition
persistent identity: BBa K180020
display id: BBa_K180020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Rock-paper-scissors - Scissors secondary plasmid [las pR, LacI generator]
creator: Richard Wilson, Stephanie Chambers
created: 2009-10-18T11:00:00Z
modified: 2015-05-08T01:11:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _278_
m user id: 0
owner id: 4082
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: iGEM2009 Team:Southampton rock-paper-scissors 'scissors' secondary plasmid.
mutable notes: Regulated expression of LacI (C0012) by Las pR (R0079).
mutable provenance: See relevant basic biobrick parts (R0070, B0034, C0012, B0015).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0015
component: BBa_B0034
component: BBa_C0012
component: BBa_R0079
sequence annotation: BBa_R0079
sequence annotation: BBa_C0012
sequence annotation: BBa_B0034
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K180020_sequence/1

BBa_B0015
type: Component
persistent identity: component2256469
display id: component2256469
version: 1
top level: BBa_K180020
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0034
type: Component
persistent identity: component2256458
display id: component2256458
version: 1
top level: BBa_K180020
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0012
type: Component
persistent identity: component2256460
display id: component2256460
version: 1
top level: BBa_K180020
access: public
definition: https://synbiohub.org/public/igem/BBa_C0012/1

BBa_R0079
type: Component
persistent identity: component2256456
display id: component2256456
version: 1
top level: BBa_K180020
access: public
definition: LasR+PAI

BBa_R0079
type: SequenceAnnotation
persistent identity: annotation2256456
display id: annotation2256456
version: 1
top level: BBa_K180020
location: https://synbiohub.org/public/igem/BBa_K180020/annotation2256456/range2256456/1
component: BBa_R0079

https://synbiohub.org/public/igem/BBa_K180020/annotation2256456/range2256456/1
type: Range
persistent identity: range2256456
display id: range2256456
version: 1
direction: direction/reverse
top level: BBa_K180020
start: 1
end: 157
orientation: inline

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation2256460
display id: annotation2256460
version: 1
top level: BBa_K180020
location: https://synbiohub.org/public/igem/BBa_K180020/annotation2256460/range2256460/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_K180020/annotation2256460/range2256460/1
type: Range
persistent identity: range2256460
display id: range2256460
version: 1
direction: direction/forward
top level: BBa_K180020
start: 184
end: 1311
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2256458
display id: annotation2256458
version: 1
top level: BBa_K180020
location: https://synbiohub.org/public/igem/BBa_K180020/annotation2256458/range2256458/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K180020/annotation2256458/range2256458/1
type: Range
persistent identity: range2256458
display id: range2256458
version: 1
direction: direction/reverse
top level: BBa_K180020
start: 166
end: 177
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2256469
display id: annotation2256469
version: 1
top level: BBa_K180020
location: https://synbiohub.org/public/igem/BBa_K180020/annotation2256469/range2256469/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K180020/annotation2256469/range2256469/1
type: Range
persistent identity: range2256469
display id: range2256469
version: 1
direction: direction/reverse
top level: BBa_K180020
start: 1345
end: 1473
orientation: inline

PN
type: ComponentDefinition
persistent identity: BBa K100000
display id: BBa_K100000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Natural Xylose Regulated Bi-Directional Operator
creator: Garrett Tobin
created: 2008-08-17T11:00:00Z
modified: 2015-05-08T01:08:41Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _213_
m user id: 0
owner id: 1752
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part contains the operator region controlling transcription for the xyl operon. This regulatory region is dependent upon the binding of CRP-cAMP and XylR bound to xylose for activation. Upon binding, transcription is activated in both directions (xylFGH and xylAB facing). This part has an unscrambled CRP binding site and and unaltered promoter region (unlike BBa_K100001 through BBa_K100003).
mutable notes: Both directions were kept in the sequence of the part even though it is intended to work in the right facing direction because of possible looping that may occur as a transcriptional control.
mutable provenance: Taken from E. Coli genome bases 3728830-3729092
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: RNA poly. binding site
sequence annotation: CRP binding site
sequence: https://synbiohub.org/public/igem/BBa_K100000_sequence/1

RNA poly. binding site
type: SequenceAnnotation
persistent identity: annotation1972263
display id: annotation1972263
version: 1
top level: PN
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K100000/annotation1972263/range1972263/1

https://synbiohub.org/public/igem/BBa_K100000/annotation1972263/range1972263/1
type: Range
persistent identity: range1972263
display id: range1972263
version: 1
direction: direction/forward
top level: PN
start: 243
end: 248
orientation: inline

RNA poly. binding site
type: SequenceAnnotation
persistent identity: annotation1972173
display id: annotation1972173
version: 1
top level: PN
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K100000/annotation1972173/range1972173/1

https://synbiohub.org/public/igem/BBa_K100000/annotation1972173/range1972173/1
type: Range
persistent identity: range1972173
display id: range1972173
version: 1
direction: direction/forward
top level: PN
start: 21
end: 26
orientation: inline

RNA poly. binding site
type: SequenceAnnotation
persistent identity: annotation1972266
display id: annotation1972266
version: 1
top level: PN
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K100000/annotation1972266/range1972266/1

https://synbiohub.org/public/igem/BBa_K100000/annotation1972266/range1972266/1
type: Range
persistent identity: range1972266
display id: range1972266
version: 1
direction: direction/forward
top level: PN
start: 264
end: 269
orientation: inline

CRP binding site
type: SequenceAnnotation
persistent identity: annotation1972261
display id: annotation1972261
version: 1
top level: PN
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K100000/annotation1972261/range1972261/1

https://synbiohub.org/public/igem/BBa_K100000/annotation1972261/range1972261/1
type: Range
persistent identity: range1972261
display id: range1972261
version: 1
direction: direction/forward
top level: PN
start: 90
end: 107
orientation: inline

RNA poly. binding site
type: SequenceAnnotation
persistent identity: annotation1972196
display id: annotation1972196
version: 1
top level: PN
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K100000/annotation1972196/range1972196/1

https://synbiohub.org/public/igem/BBa_K100000/annotation1972196/range1972196/1
type: Range
persistent identity: range1972196
display id: range1972196
version: 1
direction: direction/forward
top level: PN
start: 45
end: 50
orientation: inline

BBa_K165030
type: ComponentDefinition
persistent identity: BBa K165030
display id: BBa_K165030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mCYC promoter plus Zif268-HIV binding sites
creator: Aaron Glieberman
created: 2008-10-28T12:00:00Z
modified: 2015-05-08T01:10:56Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2510
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a composite part containing the minimal promoter mCYC preceded by multiple consecutive binding sites for the Zif268-HIV binding domain. It contains the sequences of parts BBa_K165011 and BBa_K165016 but also contains additional DNA.
mutable notes: It was necessary to design primers for use in PCR to remove this part from its host plasmid and transfer it to a standard Biobrick vector.
mutable provenance: Composite part that was taken via PCR from a synthetic plasmid containing the sequence. The plasmid came from Lawrence Berkeley National Laboratory.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Zif268-HIV binding sites (3)
sequence: https://synbiohub.org/public/igem/BBa_K165030_sequence/1

Zif268-HIV binding sites (3)
type: SequenceAnnotation
persistent identity: annotation1994037
display id: annotation1994037
version: 1
top level: BBa_K165030
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K165030/annotation1994037/range1994037/1

https://synbiohub.org/public/igem/BBa_K165030/annotation1994037/range1994037/1
type: Range
persistent identity: range1994037
display id: range1994037
version: 1
direction: direction/forward
top level: BBa_K165030
start: 1
end: 46
orientation: inline

PAtpI
type: ComponentDefinition
persistent identity: BBa K1930005
display id: BBa_K1930005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PAtpI
creator: Eike Mahlandt
created: 2016-10-15T11:00:00Z
modified: 2016-10-16T04:58:48Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2397_
m user id: 29748
owner id: 29748
owning group id: 9
status: status/Planning
bookmark: false
mutable description: The promoter PAtpI has its origin in Bacillus subtilis. It is responsible for the expression of atpA gene (ATP synthesis) during the first 30 min of germination [https://www.ncbi.nlm.nih.gov/pubmed/25661487 Sinai''et al.'']. This gene is part of an operon, therefore the promoter region in front of the first protein coding gene (atpI) in this operon was chosen. The region was checked for the binding of sigma factors and transcription factors with DBTBS. No binding factors were found with the highest significance level. In our project we wanted to find a constitutive ON promoter for our ciprofloxacin resistance casette. In the following part we put the promoter PAtpI in the pSB1C3 backbone to make it available to other iGEM teams.
mutable notes: The promoter PAtpI has its origin in Bacillus subtilis. It is responsible for the expression of atpA gene (ATP synthesis) during the first 30 min of germination [https://www.ncbi.nlm.nih.gov/pubmed/25661487 Sinai''et al.'']. This gene is part of an operon, therefore the promoter region in front of the first protein coding gene (atpI) in this operon was chosen. The region was checked for the binding of sigma factors and transcription factors with DBTBS. No binding factors were found with the highest significance level. In our project we wanted to find a constitutive ON promoter for our ciprofloxacin resistance casette. In the following part we put the promoter PAtpI in the pSB1C3 backbone to make it available to other iGEM teams.
mutable provenance: Ordered as gBlock. IDT (Integrated DNA technologies).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: P(AtpI)
sequence: https://synbiohub.org/public/igem/BBa_K1930005_sequence/1

P(AtpI)
type: SequenceAnnotation
persistent identity: annotation2513454
display id: annotation2513454
version: 1
top level: PAtpI
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1930005/annotation2513454/range2513454/1

https://synbiohub.org/public/igem/BBa_K1930005/annotation2513454/range2513454/1
type: Range
persistent identity: range2513454
display id: range2513454
version: 1
direction: direction/forward
top level: PAtpI
start: 1
end: 349
orientation: inline

LasR+PAI
type: ComponentDefinition
persistent identity: BBa R0079
display id: BBa_R0079
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (LasR & PAI regulated)
creator: Alvin Carter Powers (Fighting Darwins)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Binding region for LasR protein (positive regulation)
mutable provenance: "Analysis of the Pseudomonas aeruginosa Elastase (lasB) Regulatory Region". Lynn Rust, Everett Pesci, and Barbara Iglewski
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: OP2
sequence annotation: -35
sequence annotation: OP1
sequence: https://synbiohub.org/public/igem/BBa_R0079_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation318496
display id: annotation318496
version: 1
top level: LasR+PAI
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0079/annotation318496/range318496/1

https://synbiohub.org/public/igem/BBa_R0079/annotation318496/range318496/1
type: Range
persistent identity: range318496
display id: range318496
version: 1
direction: direction/forward
top level: LasR+PAI
start: 140
end: 145
orientation: inline

OP2
type: SequenceAnnotation
persistent identity: annotation300985
display id: annotation300985
version: 1
top level: LasR+PAI
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0079/annotation300985/range300985/1

https://synbiohub.org/public/igem/BBa_R0079/annotation300985/range300985/1
type: Range
persistent identity: range300985
display id: range300985
version: 1
direction: direction/forward
top level: LasR+PAI
start: 46
end: 63
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation318495
display id: annotation318495
version: 1
top level: LasR+PAI
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0079/annotation318495/range318495/1

https://synbiohub.org/public/igem/BBa_R0079/annotation318495/range318495/1
type: Range
persistent identity: range318495
display id: range318495
version: 1
direction: direction/forward
top level: LasR+PAI
start: 117
end: 122
orientation: inline

OP1
type: SequenceAnnotation
persistent identity: annotation300992
display id: annotation300992
version: 1
top level: LasR+PAI
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0079/annotation300992/range300992/1

https://synbiohub.org/public/igem/BBa_R0079/annotation300992/range300992/1
type: Range
persistent identity: range300992
display id: range300992
version: 1
direction: direction/forward
top level: LasR+PAI
start: 106
end: 125
orientation: inline

BBa_J16105
type: ComponentDefinition
persistent identity: BBa J16105
display id: BBa_J16105
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HelPp - "Help" Dependant promoter
creator: James Brown
created: 2006-07-23T11:00:00Z
modified: 2015-08-31T04:08:33Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 740
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Transciptional regulator reliant on screams of someone, somewhere in trouble.
mutable notes: Very, Very sensitive
mutable provenance: DNA sample - Taken from Clark Kent's Coffee Mug at the Daily Planet
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J16105_sequence/1