//regulation/multiple
type: Collection
persistent identity: regulation multiple
display id: regulation_multiple
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_K1486013
member: BBa_K110016
member: NAND2717
member: U073O11+D0
member: BBa_K091105
member: BBa_I732451
member: BBa_I12036
member: U097NUL+D0
member: BBa_K259005
member: U085O11+D0
member: Prm +
member: BBa_K608351
member: BBa_K1486055
member: BBa_K283018
member: Plac(rev)
member: BBa_K112406
member: BBa_K136004
member: U049O11+D0
member: U061O11+D0
member: glnAp2 pro
member: U037O11+D0
member: BBa_I739104
member: U097O11+D0
member: BBa_K1125002
member: BBa_K266007
member: T7/lac pro
member: BBa_K1486040
member: BBa_K1486010
member: BBa_I739103
member: BBa_K112701
member: BBa_K112322
member: BBa_K109200
member: las TetO
member: BBa_I751501
member: BBa_K145150
member: NOR2
member: BBa_K136010
member: BBa_I739106
member: BBa_K1486012
member: BBa_K101002
member: BBa_K091101
member: BBa_K091157
member: BBa_I714924
member: NAND01
member: BBa_K266006
member: BBa_I765007
member: BBa_K121011
member: BBa_I732450
member: BBa_I731004
member: BBa_J05216
member: BBa_K112402
member: BBa_K177038
member: BBa_K1486008
member: NAND59
member: BBa_K1486001

U037O11+D0
type: ComponentDefinition
persistent identity: BBa I732446
display id: BBa_I732446
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U037O11+D038O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732446_sequence/1

BBa_I751501
type: ComponentDefinition
persistent identity: BBa I751501
display id: BBa_I751501
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: plux-cI hybrid promoter
creator: Kenichiro Iwasaki
created: 2007-10-24T11:00:00Z
modified: 2015-08-31T04:08:06Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _151_
m user id: 0
owner id: 2188
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: sense TWO INPUTS, activation by AHL and repression by LacI.
mutable notes: Based on the AHL regulated BioBrick part R0062 and the previous work on LacI regulated promoter (ref. 1), we designed a new promoter regulated by AHL and LacI. Since this promoter is a kind of a "HYBRID" of the two, we call it HYBRID PROMOTER. Compared with existing BioBrick hybrid promoter R0065, this promoter is different in that its sequence is much shorter with lux box and -35 region overlapping, and that it is repressed by LacI, not cI. Also, the output is clearly detected as shown in Fig. 3.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OR1
sequence annotation: OR2
sequence annotation: luxR binding
sequence annotation: plux-cI hybrid promoter
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_I751501_sequence/1

OR1
type: SequenceAnnotation
persistent identity: annotation1955477
display id: annotation1955477
version: 1
top level: BBa_I751501
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I751501/annotation1955477/range1955477/1

https://synbiohub.org/public/igem/BBa_I751501/annotation1955477/range1955477/1
type: Range
persistent identity: range1955477
display id: range1955477
version: 1
direction: direction/forward
top level: BBa_I751501
start: 48
end: 66
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation1955481
display id: annotation1955481
version: 1
top level: BBa_I751501
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I751501/annotation1955481/range1955481/1

https://synbiohub.org/public/igem/BBa_I751501/annotation1955481/range1955481/1
type: Range
persistent identity: range1955481
display id: range1955481
version: 1
direction: direction/forward
top level: BBa_I751501
start: 26
end: 41
orientation: inline

luxR binding
type: SequenceAnnotation
persistent identity: annotation1955482
display id: annotation1955482
version: 1
top level: BBa_I751501
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I751501/annotation1955482/range1955482/1

https://synbiohub.org/public/igem/BBa_I751501/annotation1955482/range1955482/1
type: Range
persistent identity: range1955482
display id: range1955482
version: 1
direction: direction/forward
top level: BBa_I751501
start: 1
end: 18
orientation: inline

plux-cI hybrid promoter
type: SequenceAnnotation
persistent identity: annotation1955478
display id: annotation1955478
version: 1
top level: BBa_I751501
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I751501/annotation1955478/range1955478/1

https://synbiohub.org/public/igem/BBa_I751501/annotation1955478/range1955478/1
type: Range
persistent identity: range1955478
display id: range1955478
version: 1
direction: direction/forward
top level: BBa_I751501
start: 19
end: 66
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1955479
display id: annotation1955479
version: 1
top level: BBa_I751501
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I751501/annotation1955479/range1955479/1

https://synbiohub.org/public/igem/BBa_I751501/annotation1955479/range1955479/1
type: Range
persistent identity: range1955479
display id: range1955479
version: 1
direction: direction/forward
top level: BBa_I751501
start: 19
end: 25
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1955480
display id: annotation1955480
version: 1
top level: BBa_I751501
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I751501/annotation1955480/range1955480/1

https://synbiohub.org/public/igem/BBa_I751501/annotation1955480/range1955480/1
type: Range
persistent identity: range1955480
display id: range1955480
version: 1
direction: direction/forward
top level: BBa_I751501
start: 42
end: 47
orientation: inline

U073O11+D0
type: ComponentDefinition
persistent identity: BBa I732425
display id: BBa_I732425
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U073O11+D002O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: true
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732425_sequence/1

BBa_K1486041
type: ComponentDefinition
persistent identity: BBa K1486041
display id: BBa_K1486041
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sfGFPC + CpxR
creator: Ione Pla Puigsubir??
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_I0500
component: BBa_K1486037
component: BBa_K1486039
component: BBa_B0034
component: BBa_K1486000
sequence annotation: BBa_K1486039
sequence annotation: BBa_I0500
sequence annotation: BBa_B0034
sequence annotation: BBa_K1486037
sequence annotation: BBa_K1486000
sequence: https://synbiohub.org/public/igem/BBa_K1486041_sequence/1

BBa_I0500
type: Component
persistent identity: component2387209
display id: component2387209
version: 1
top level: BBa_K1486041
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_K1486037
type: Component
persistent identity: component2387213
display id: component2387213
version: 1
top level: BBa_K1486041
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486037/1

BBa_K1486039
type: Component
persistent identity: component2387212
display id: component2387212
version: 1
top level: BBa_K1486041
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486039/1

BBa_B0034
type: Component
persistent identity: component2387211
display id: component2387211
version: 1
top level: BBa_K1486041
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486000
type: Component
persistent identity: component2387217
display id: component2387217
version: 1
top level: BBa_K1486041
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_K1486039
type: SequenceAnnotation
persistent identity: annotation2387212
display id: annotation2387212
version: 1
top level: BBa_K1486041
location: https://synbiohub.org/public/igem/BBa_K1486041/annotation2387212/range2387212/1
component: BBa_K1486039

https://synbiohub.org/public/igem/BBa_K1486041/annotation2387212/range2387212/1
type: Range
persistent identity: range2387212
display id: range2387212
version: 1
direction: direction/reverse
top level: BBa_K1486041
start: 1223
end: 1294
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2387209
display id: annotation2387209
version: 1
top level: BBa_K1486041
location: https://synbiohub.org/public/igem/BBa_K1486041/annotation2387209/range2387209/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486041/annotation2387209/range2387209/1
type: Range
persistent identity: range2387209
display id: range2387209
version: 1
direction: direction/reverse
top level: BBa_K1486041
start: 1
end: 1210
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2387211
display id: annotation2387211
version: 1
top level: BBa_K1486041
location: https://synbiohub.org/public/igem/BBa_K1486041/annotation2387211/range2387211/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486041/annotation2387211/range2387211/1
type: Range
persistent identity: range2387211
display id: range2387211
version: 1
direction: direction/reverse
top level: BBa_K1486041
start: 1211
end: 1222
orientation: inline

BBa_K1486037
type: SequenceAnnotation
persistent identity: annotation2387213
display id: annotation2387213
version: 1
top level: BBa_K1486041
location: https://synbiohub.org/public/igem/BBa_K1486041/annotation2387213/range2387213/1
component: BBa_K1486037

https://synbiohub.org/public/igem/BBa_K1486041/annotation2387213/range2387213/1
type: Range
persistent identity: range2387213
display id: range2387213
version: 1
direction: direction/reverse
top level: BBa_K1486041
start: 1295
end: 1333
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2387217
display id: annotation2387217
version: 1
top level: BBa_K1486041
location: https://synbiohub.org/public/igem/BBa_K1486041/annotation2387217/range2387217/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486041/annotation2387217/range2387217/1
type: Range
persistent identity: range2387217
display id: range2387217
version: 1
direction: direction/reverse
top level: BBa_K1486041
start: 1334
end: 2035
orientation: inline

BBa_K259007
type: ComponentDefinition
persistent identity: BBa K259007
display id: BBa_K259007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AraC Promoter fused with RBS
creator: Petros Mina
created: 2009-09-08T11:00:00Z
modified: 2015-05-08T01:11:42Z
discontinued: false
dominant: false
group u list: _357_
m user id: 0
owner id: 5318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part has the arabinose regulated promoter (AraC/pBAD) upstream of a ribosome binding site. This is ideal for inserting a protein to be expressed downstream of this part as it's expression can be regulated by the presence of the concentration of arabinose in the growing environment/media of the cells. ==What this part actually does== This composite part includes the AraC/pBAD promoter and a strong ribosome binding site designed from the Anderson family of RBS. The AraC/pBAD promoter can be used with prokaryotic chassis to regulate expresion of proteins. When the AraC/pBAD promoter is placed upstream of your canditate translational unit (protein to be expressed) it allows control of the trancription of the said protein. This is done via controlling the concentration of arabinose sugar in the environment/solution of bacterial growth. Controlling the transcription will not ensure translation. The ribosome binding site is ideally placed a small distance away from the promoter site to ensure that the ribosome will bind and start translating the protein coding sequence found downstream.
mutable notes: The part was designed with RFC10 assembly from supplied biobricks of the iGEM2009 distribution.
mutable provenance: The AraC promoter can be found naturally at 1.52min of the E.coli genome ([http://ecogene.org/geneinfo.php?eg_id=EG10054 EcoGene number EG10054]. The RBS was taken from the designed sequences of RBS by the Anderson family. For more information visit the original part page [http://partsregistry.org/Part:BBa_J61100 here].
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_R0080
component: BBa_J61100
component: BBa_R0081
sequence annotation: BBa_R0080
sequence annotation: BBa_R0081
sequence annotation: BBa_J61100
sequence: https://synbiohub.org/public/igem/BBa_K259007_sequence/1

BBa_R0080
type: Component
persistent identity: component2020546
display id: component2020546
version: 1
top level: BBa_K259007
access: public
definition: https://synbiohub.org/public/igem/BBa_R0080/1

BBa_J61100
type: Component
persistent identity: component2020547
display id: component2020547
version: 1
top level: BBa_K259007
access: public
definition: https://synbiohub.org/public/igem/BBa_J61100/1

BBa_R0081
type: Component
persistent identity: component2020539
display id: component2020539
version: 1
top level: BBa_K259007
access: public
definition: https://synbiohub.org/public/igem/BBa_R0081/1

BBa_R0080
type: SequenceAnnotation
persistent identity: annotation2020546
display id: annotation2020546
version: 1
top level: BBa_K259007
location: https://synbiohub.org/public/igem/BBa_K259007/annotation2020546/range2020546/1
component: BBa_R0080

https://synbiohub.org/public/igem/BBa_K259007/annotation2020546/range2020546/1
type: Range
persistent identity: range2020546
display id: range2020546
version: 1
direction: direction/reverse
top level: BBa_K259007
start: 192
end: 340
orientation: inline

BBa_R0081
type: SequenceAnnotation
persistent identity: annotation2020539
display id: annotation2020539
version: 1
top level: BBa_K259007
location: https://synbiohub.org/public/igem/BBa_K259007/annotation2020539/range2020539/1
component: BBa_R0081

https://synbiohub.org/public/igem/BBa_K259007/annotation2020539/range2020539/1
type: Range
persistent identity: range2020539
display id: range2020539
version: 1
direction: direction/reverse
top level: BBa_K259007
start: 1
end: 183
orientation: inline

BBa_J61100
type: SequenceAnnotation
persistent identity: annotation2020547
display id: annotation2020547
version: 1
top level: BBa_K259007
location: https://synbiohub.org/public/igem/BBa_K259007/annotation2020547/range2020547/1
component: BBa_J61100

https://synbiohub.org/public/igem/BBa_K259007/annotation2020547/range2020547/1
type: Range
persistent identity: range2020547
display id: range2020547
version: 1
direction: direction/reverse
top level: BBa_K259007
start: 349
end: 360
orientation: inline

U085O11+D0
type: ComponentDefinition
persistent identity: BBa I732423
display id: BBa_I732423
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U085O11+D050O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732423_sequence/1

BBa_K608351
type: ComponentDefinition
persistent identity: BBa K608351
display id: BBa_K608351
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: (correct) temperature sensitive promoter
creator: Theodhor Tiko
created: 2011-09-13T11:00:00Z
modified: 2015-05-08T01:12:52Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _780_
m user id: 0
owner id: 9113
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: correct temperatursesitive promotor
mutable notes: asd
mutable provenance: asd
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: polya
sequence annotation: conserved
sequence annotation: OR1
sequence annotation: BBa_K098997
sequence annotation: BBa_J23114
sequence annotation: BBa_B0012
sequence annotation: cIts
sequence annotation: stop
sequence annotation: BBa_R0051
sequence annotation: -10
sequence annotation: BBa_B0034
sequence annotation: OR2
sequence annotation: BBa_B0011
sequence annotation: stem_loop
sequence annotation: -35
sequence annotation: T7 TE
sequence: https://synbiohub.org/public/igem/BBa_K608351_sequence/1

polya
type: SequenceAnnotation
persistent identity: annotation2131307
display id: annotation2131307
version: 1
top level: BBa_K608351
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131307/range2131307/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131307/range2131307/1
type: Range
persistent identity: range2131307
display id: range2131307
version: 1
direction: direction/forward
top level: BBa_K608351
start: 824
end: 837
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2131425
display id: annotation2131425
version: 1
top level: BBa_K608351
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131425/range2131425/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131425/range2131425/1
type: Range
persistent identity: range2131425
display id: range2131425
version: 1
direction: direction/forward
top level: BBa_K608351
start: 776
end: 788
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation2131314
display id: annotation2131314
version: 1
top level: BBa_K608351
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131314/range2131314/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131314/range2131314/1
type: Range
persistent identity: range2131314
display id: range2131314
version: 1
direction: direction/forward
top level: BBa_K608351
start: 924
end: 940
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2131301
display id: annotation2131301
version: 1
top level: BBa_K608351
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131301/range2131301/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131301/range2131301/1
type: Range
persistent identity: range2131301
display id: range2131301
version: 1
direction: direction/forward
top level: BBa_K608351
start: 48
end: 51
orientation: inline

BBa_K098997
type: SequenceAnnotation
persistent identity: annotation2131304
display id: annotation2131304
version: 1
top level: BBa_K608351
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131304/range2131304/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131304/range2131304/1
type: Range
persistent identity: range2131304
display id: range2131304
version: 1
direction: direction/reverse
top level: BBa_K608351
start: 62
end: 775
orientation: inline

BBa_J23114
type: SequenceAnnotation
persistent identity: annotation2131300
display id: annotation2131300
version: 1
top level: BBa_K608351
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131300/range2131300/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131300/range2131300/1
type: Range
persistent identity: range2131300
display id: range2131300
version: 1
direction: direction/reverse
top level: BBa_K608351
start: 1
end: 35
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2131305
display id: annotation2131305
version: 1
top level: BBa_K608351
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131305/range2131305/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131305/range2131305/1
type: Range
persistent identity: range2131305
display id: range2131305
version: 1
direction: direction/forward
top level: BBa_K608351
start: 797
end: 837
orientation: inline

cIts
type: SequenceAnnotation
persistent identity: annotation2131303
display id: annotation2131303
version: 1
top level: BBa_K608351
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131303/range2131303/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131303/range2131303/1
type: Range
persistent identity: range2131303
display id: range2131303
version: 1
direction: direction/forward
top level: BBa_K608351
start: 62
end: 775
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2131308
display id: annotation2131308
version: 1
top level: BBa_K608351
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131308/range2131308/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131308/range2131308/1
type: Range
persistent identity: range2131308
display id: range2131308
version: 1
direction: direction/forward
top level: BBa_K608351
start: 830
end: 830
orientation: inline

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation2131312
display id: annotation2131312
version: 1
top level: BBa_K608351
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131312/range2131312/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131312/range2131312/1
type: Range
persistent identity: range2131312
display id: range2131312
version: 1
direction: direction/forward
top level: BBa_K608351
start: 900
end: 948
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2131315
display id: annotation2131315
version: 1
top level: BBa_K608351
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131315/range2131315/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131315/range2131315/1
type: Range
persistent identity: range2131315
display id: range2131315
version: 1
direction: direction/forward
top level: BBa_K608351
start: 937
end: 942
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2131302
display id: annotation2131302
version: 1
top level: BBa_K608351
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131302/range2131302/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131302/range2131302/1
type: Range
persistent identity: range2131302
display id: range2131302
version: 1
direction: direction/reverse
top level: BBa_K608351
start: 44
end: 55
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2131311
display id: annotation2131311
version: 1
top level: BBa_K608351
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131311/range2131311/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131311/range2131311/1
type: Range
persistent identity: range2131311
display id: range2131311
version: 1
direction: direction/forward
top level: BBa_K608351
start: 900
end: 916
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation2131309
display id: annotation2131309
version: 1
top level: BBa_K608351
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131309/range2131309/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131309/range2131309/1
type: Range
persistent identity: range2131309
display id: range2131309
version: 1
direction: direction/forward
top level: BBa_K608351
start: 846
end: 891
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2131310
display id: annotation2131310
version: 1
top level: BBa_K608351
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131310/range2131310/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131310/range2131310/1
type: Range
persistent identity: range2131310
display id: range2131310
version: 1
direction: direction/forward
top level: BBa_K608351
start: 858
end: 880
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2131313
display id: annotation2131313
version: 1
top level: BBa_K608351
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131313/range2131313/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131313/range2131313/1
type: Range
persistent identity: range2131313
display id: range2131313
version: 1
direction: direction/forward
top level: BBa_K608351
start: 914
end: 919
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2131306
display id: annotation2131306
version: 1
top level: BBa_K608351
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131306/range2131306/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131306/range2131306/1
type: Range
persistent identity: range2131306
display id: range2131306
version: 1
direction: direction/forward
top level: BBa_K608351
start: 804
end: 823
orientation: inline

T7/lac pro
type: ComponentDefinition
persistent identity: BBa K1362463
display id: BBa_K1362463
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: strong T7 promoter + LacO Regulator
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 22830
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: GAATTTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCC
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: strong T7 promotor
sequence annotation: scar of cloning into EcoRI site
sequence annotation: lacO reg
sequence: https://synbiohub.org/public/igem/BBa_K1362463_sequence/1

strong T7 promotor
type: SequenceAnnotation
persistent identity: annotation2405261
display id: annotation2405261
version: 1
top level: T7/lac pro
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1362463/annotation2405261/range2405261/1

https://synbiohub.org/public/igem/BBa_K1362463/annotation2405261/range2405261/1
type: Range
persistent identity: range2405261
display id: range2405261
version: 1
direction: direction/forward
top level: T7/lac pro
start: 6
end: 24
orientation: inline

scar of cloning into EcoRI site
type: SequenceAnnotation
persistent identity: annotation2405286
display id: annotation2405286
version: 1
top level: T7/lac pro
role: feature/scar
location: https://synbiohub.org/public/igem/BBa_K1362463/annotation2405286/range2405286/1

https://synbiohub.org/public/igem/BBa_K1362463/annotation2405286/range2405286/1
type: Range
persistent identity: range2405286
display id: range2405286
version: 1
direction: direction/forward
top level: T7/lac pro
start: 1
end: 6
orientation: inline

lacO reg
type: SequenceAnnotation
persistent identity: annotation2405276
display id: annotation2405276
version: 1
top level: T7/lac pro
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1362463/annotation2405276/range2405276/1

https://synbiohub.org/public/igem/BBa_K1362463/annotation2405276/range2405276/1
type: Range
persistent identity: range2405276
display id: range2405276
version: 1
direction: direction/forward
top level: T7/lac pro
start: 24
end: 51
orientation: inline

BBa_K1486014
type: ComponentDefinition
persistent identity: BBa K1486014
display id: BBa_K1486014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IFP[1]-CpxR under pBAD
creator: iGem EPFL 2014
created: 2014-08-31T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part has a fused protein downstream of an arabinose-inducible promoter: IFP[1]-CpxR. Biobrick BBa_K1486015 has IFP[2]-CpxR with an arabinose promoter. This construct was made in both orientations: BBa_K1486012 is CpxR-IFP[1] (with an arabinose promoter).
mutable notes: Both orientations were made, as well as for IFP[2] so all dimerizations could be tested (see main page for biobrick numbers). Flexible linkers were used to fuse both proteins, and an RBS was put between the promoter and the cds.
mutable provenance: to edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1486004
component: BBa_I0500
component: BBa_B0034
component: BBa_K1486000
component: BBa_K1486006
sequence annotation: BBa_K1486006
sequence annotation: BBa_K1486004
sequence annotation: BBa_B0034
sequence annotation: BBa_K1486000
sequence annotation: BBa_I0500
sequence: https://synbiohub.org/public/igem/BBa_K1486014_sequence/1

BBa_K1486004
type: Component
persistent identity: component2424743
display id: component2424743
version: 1
top level: BBa_K1486014
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_I0500
type: Component
persistent identity: component2424736
display id: component2424736
version: 1
top level: BBa_K1486014
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_B0034
type: Component
persistent identity: component2424738
display id: component2424738
version: 1
top level: BBa_K1486014
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486000
type: Component
persistent identity: component2424747
display id: component2424747
version: 1
top level: BBa_K1486014
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_K1486006
type: Component
persistent identity: component2424742
display id: component2424742
version: 1
top level: BBa_K1486014
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486006/1

BBa_K1486006
type: SequenceAnnotation
persistent identity: annotation2424742
display id: annotation2424742
version: 1
top level: BBa_K1486014
location: https://synbiohub.org/public/igem/BBa_K1486014/annotation2424742/range2424742/1
component: BBa_K1486006

https://synbiohub.org/public/igem/BBa_K1486014/annotation2424742/range2424742/1
type: Range
persistent identity: range2424742
display id: range2424742
version: 1
direction: direction/reverse
top level: BBa_K1486014
start: 1223
end: 1627
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2424743
display id: annotation2424743
version: 1
top level: BBa_K1486014
location: https://synbiohub.org/public/igem/BBa_K1486014/annotation2424743/range2424743/1
component: BBa_K1486004

https://synbiohub.org/public/igem/BBa_K1486014/annotation2424743/range2424743/1
type: Range
persistent identity: range2424743
display id: range2424743
version: 1
direction: direction/reverse
top level: BBa_K1486014
start: 1628
end: 1657
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2424738
display id: annotation2424738
version: 1
top level: BBa_K1486014
location: https://synbiohub.org/public/igem/BBa_K1486014/annotation2424738/range2424738/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486014/annotation2424738/range2424738/1
type: Range
persistent identity: range2424738
display id: range2424738
version: 1
direction: direction/reverse
top level: BBa_K1486014
start: 1211
end: 1222
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2424747
display id: annotation2424747
version: 1
top level: BBa_K1486014
location: https://synbiohub.org/public/igem/BBa_K1486014/annotation2424747/range2424747/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486014/annotation2424747/range2424747/1
type: Range
persistent identity: range2424747
display id: range2424747
version: 1
direction: direction/reverse
top level: BBa_K1486014
start: 1658
end: 2359
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2424736
display id: annotation2424736
version: 1
top level: BBa_K1486014
location: https://synbiohub.org/public/igem/BBa_K1486014/annotation2424736/range2424736/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486014/annotation2424736/range2424736/1
type: Range
persistent identity: range2424736
display id: range2424736
version: 1
direction: direction/reverse
top level: BBa_K1486014
start: 1
end: 1210
orientation: inline

U097O11+D0
type: ComponentDefinition
persistent identity: BBa I732416
display id: BBa_I732416
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U097O11+D038O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732416_sequence/1

Prm +
type: ComponentDefinition
persistent identity: BBa I12006
display id: BBa_I12006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Modified lamdba Prm promoter (repressed by 434 cI)
creator: mcnamara
created: 2004-07-13T11:00:00Z
modified: 2015-08-31T04:07:31Z
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 147
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Lamdba Prm promoter modified to be activated by lamda repressor (cI) and repressed by 434 repressor (cI)
mutable notes: The O-R1 region of 434 contained 14 base pairs as opposed to the 17 base pairs of the O-R3 site of lambda. Also, it was noticed that the O-R3 site of the lambda included part of the -10 site. Hence, to preserve the spacing and the -10 site, the three nucleotides that were in both the -10 site and the lambda O-R3 site were retained. The 14 nucleotides that were in the O-R3 site and not in the -10 site were replaced with the O-R1 site of the 434.
mutable provenance: Bushman(1993), Shih & Gussin (1983)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OR1 lambda
sequence annotation: OR2 lambda
sequence annotation: -10
sequence annotation: OR1 434
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_I12006_sequence/1

OR1 lambda
type: SequenceAnnotation
persistent identity: annotation786500
display id: annotation786500
version: 1
top level: Prm +
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12006/annotation786500/range786500/1

https://synbiohub.org/public/igem/BBa_I12006/annotation786500/range786500/1
type: Range
persistent identity: range786500
display id: range786500
version: 1
direction: direction/forward
top level: Prm +
start: 9
end: 25
orientation: inline

OR2 lambda
type: SequenceAnnotation
persistent identity: annotation786518
display id: annotation786518
version: 1
top level: Prm +
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12006/annotation786518/range786518/1

https://synbiohub.org/public/igem/BBa_I12006/annotation786518/range786518/1
type: Range
persistent identity: range786518
display id: range786518
version: 1
direction: direction/forward
top level: Prm +
start: 33
end: 49
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation837228
display id: annotation837228
version: 1
top level: Prm +
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I12006/annotation837228/range837228/1

https://synbiohub.org/public/igem/BBa_I12006/annotation837228/range837228/1
type: Range
persistent identity: range837228
display id: range837228
version: 1
direction: direction/forward
top level: Prm +
start: 71
end: 76
orientation: inline

OR1 434
type: SequenceAnnotation
persistent identity: annotation786365
display id: annotation786365
version: 1
top level: Prm +
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12006/annotation786365/range786365/1

https://synbiohub.org/public/igem/BBa_I12006/annotation786365/range786365/1
type: Range
persistent identity: range786365
display id: range786365
version: 1
direction: direction/forward
top level: Prm +
start: 56
end: 69
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation837284
display id: annotation837284
version: 1
top level: Prm +
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I12006/annotation837284/range837284/1

https://synbiohub.org/public/igem/BBa_I12006/annotation837284/range837284/1
type: Range
persistent identity: range837284
display id: range837284
version: 1
direction: direction/forward
top level: Prm +
start: 48
end: 53
orientation: inline

BBa_K990002
type: ComponentDefinition
persistent identity: BBa K990002
display id: BBa_K990002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IPTG Inducible LasI Device
creator: Fatma Betul CEVIK
created: 2013-06-20T11:00:00Z
modified: 2015-05-08T01:13:50Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1287_
m user id: 0
owner id: 11713
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: IPTG inducible AHL generator, LasI device.
mutable notes: Some sequence was checked NCBI
mutable provenance: pConstitutive: J23100 LasI device: K772005
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K772005
component: BBa_J23100
sequence annotation: BBa_K772005
sequence annotation: BBa_J23100
sequence: https://synbiohub.org/public/igem/BBa_K990002_sequence/1

BBa_K772005
type: Component
persistent identity: component2329865
display id: component2329865
version: 1
top level: BBa_K990002
access: public
definition: https://synbiohub.org/public/igem/BBa_K772005/1

BBa_J23100
type: Component
persistent identity: component2329859
display id: component2329859
version: 1
top level: BBa_K990002
access: public
definition: https://synbiohub.org/public/igem/BBa_J23100/1

BBa_K772005
type: SequenceAnnotation
persistent identity: annotation2329865
display id: annotation2329865
version: 1
top level: BBa_K990002
location: https://synbiohub.org/public/igem/BBa_K990002/annotation2329865/range2329865/1
component: BBa_K772005

https://synbiohub.org/public/igem/BBa_K990002/annotation2329865/range2329865/1
type: Range
persistent identity: range2329865
display id: range2329865
version: 1
direction: direction/reverse
top level: BBa_K990002
start: 44
end: 2141
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2329859
display id: annotation2329859
version: 1
top level: BBa_K990002
location: https://synbiohub.org/public/igem/BBa_K990002/annotation2329859/range2329859/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K990002/annotation2329859/range2329859/1
type: Range
persistent identity: range2329859
display id: range2329859
version: 1
direction: direction/reverse
top level: BBa_K990002
start: 1
end: 35
orientation: inline

BBa_K136010
type: ComponentDefinition
persistent identity: BBa K136010
display id: BBa_K136010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: fliA promoter
creator: Kok-Phen YAN
created: 2008-10-24T11:00:00Z
modified: 2015-05-08T01:10:02Z
discontinued: false
dominant: false
group u list: _211_
m user id: 0
owner id: 3262
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: FliA is a transcription factor involved in the formation of the flagella in E. coli. The promotor of fliA is induced by FlhDC. FliA seems to have a positive autoregulation on its promotor.
mutable notes: primers used
mutable provenance: This part comes from the genome of E. coli K12 strain MG1655.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: transcription initiation site
sequence annotation: natural fliA RBS
sequence annotation: A mutated to G
sequence annotation: FlhDC binding site 2
sequence annotation: ATG
sequence annotation: FlhDC binding site 1
sequence: https://synbiohub.org/public/igem/BBa_K136010_sequence/1

transcription initiation site
type: SequenceAnnotation
persistent identity: annotation1987046
display id: annotation1987046
version: 1
top level: BBa_K136010
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K136010/annotation1987046/range1987046/1

https://synbiohub.org/public/igem/BBa_K136010/annotation1987046/range1987046/1
type: Range
persistent identity: range1987046
display id: range1987046
version: 1
direction: direction/forward
top level: BBa_K136010
start: 293
end: 293
orientation: inline

natural fliA RBS
type: SequenceAnnotation
persistent identity: annotation1999348
display id: annotation1999348
version: 1
top level: BBa_K136010
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K136010/annotation1999348/range1999348/1

https://synbiohub.org/public/igem/BBa_K136010/annotation1999348/range1999348/1
type: Range
persistent identity: range1999348
display id: range1999348
version: 1
direction: direction/forward
top level: BBa_K136010
start: 330
end: 337
orientation: inline

A mutated to G
type: SequenceAnnotation
persistent identity: annotation1987042
display id: annotation1987042
version: 1
top level: BBa_K136010
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K136010/annotation1987042/range1987042/1

https://synbiohub.org/public/igem/BBa_K136010/annotation1987042/range1987042/1
type: Range
persistent identity: range1987042
display id: range1987042
version: 1
direction: direction/forward
top level: BBa_K136010
start: 263
end: 263
orientation: inline

transcription initiation site
type: SequenceAnnotation
persistent identity: annotation1987045
display id: annotation1987045
version: 1
top level: BBa_K136010
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K136010/annotation1987045/range1987045/1

https://synbiohub.org/public/igem/BBa_K136010/annotation1987045/range1987045/1
type: Range
persistent identity: range1987045
display id: range1987045
version: 1
direction: direction/forward
top level: BBa_K136010
start: 282
end: 282
orientation: inline

A mutated to G
type: SequenceAnnotation
persistent identity: annotation1987043
display id: annotation1987043
version: 1
top level: BBa_K136010
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K136010/annotation1987043/range1987043/1

https://synbiohub.org/public/igem/BBa_K136010/annotation1987043/range1987043/1
type: Range
persistent identity: range1987043
display id: range1987043
version: 1
direction: direction/forward
top level: BBa_K136010
start: 316
end: 316
orientation: inline

FlhDC binding site 2
type: SequenceAnnotation
persistent identity: annotation1987041
display id: annotation1987041
version: 1
top level: BBa_K136010
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K136010/annotation1987041/range1987041/1

https://synbiohub.org/public/igem/BBa_K136010/annotation1987041/range1987041/1
type: Range
persistent identity: range1987041
display id: range1987041
version: 1
direction: direction/forward
top level: BBa_K136010
start: 226
end: 261
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation1987044
display id: annotation1987044
version: 1
top level: BBa_K136010
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K136010/annotation1987044/range1987044/1

https://synbiohub.org/public/igem/BBa_K136010/annotation1987044/range1987044/1
type: Range
persistent identity: range1987044
display id: range1987044
version: 1
direction: direction/forward
top level: BBa_K136010
start: 342
end: 344
orientation: inline

FlhDC binding site 1
type: SequenceAnnotation
persistent identity: annotation1987040
display id: annotation1987040
version: 1
top level: BBa_K136010
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K136010/annotation1987040/range1987040/1

https://synbiohub.org/public/igem/BBa_K136010/annotation1987040/range1987040/1
type: Range
persistent identity: range1987040
display id: range1987040
version: 1
direction: direction/forward
top level: BBa_K136010
start: 198
end: 225
orientation: inline

BBa_K266006
type: ComponentDefinition
persistent identity: BBa K266006
display id: BBa_K266006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PAI+LasR -> LasI+GFP & AI+LuxR --| LasI+GFP
creator: UNAM FCIENCIAS-CINVESTAV IRA
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _363_
m user id: 0
owner id: 1955
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: BBa_K091146 promoter (PAI+LasR inducible & AI+LuxR repressible) controls the policistronic expresion of LasI enzyme and GFP, double terminator
mutable notes: None.
mutable provenance: The biobricks BBa_K266005 and BBa_E0840 where used to build this biobrick.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_K266005
component: BBa_E0840
sequence annotation: BBa_E0840
sequence annotation: BBa_K266005
sequence: https://synbiohub.org/public/igem/BBa_K266006_sequence/1

BBa_K266005
type: Component
persistent identity: component2267607
display id: component2267607
version: 1
top level: BBa_K266006
access: public
definition: BBa_K266005

BBa_E0840
type: Component
persistent identity: component2267618
display id: component2267618
version: 1
top level: BBa_K266006
access: public
definition: https://synbiohub.org/public/igem/BBa_E0840/1

BBa_E0840
type: SequenceAnnotation
persistent identity: annotation2267618
display id: annotation2267618
version: 1
top level: BBa_K266006
location: https://synbiohub.org/public/igem/BBa_K266006/annotation2267618/range2267618/1
component: BBa_E0840

https://synbiohub.org/public/igem/BBa_K266006/annotation2267618/range2267618/1
type: Range
persistent identity: range2267618
display id: range2267618
version: 1
direction: direction/reverse
top level: BBa_K266006
start: 828
end: 1705
orientation: inline

BBa_K266005
type: SequenceAnnotation
persistent identity: annotation2267607
display id: annotation2267607
version: 1
top level: BBa_K266006
location: https://synbiohub.org/public/igem/BBa_K266006/annotation2267607/range2267607/1
component: BBa_K266005

https://synbiohub.org/public/igem/BBa_K266006/annotation2267607/range2267607/1
type: Range
persistent identity: range2267607
display id: range2267607
version: 1
direction: direction/reverse
top level: BBa_K266006
start: 1
end: 819
orientation: inline

U049O11+D0
type: ComponentDefinition
persistent identity: BBa I732438
display id: BBa_I732438
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U049O11+D014O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732438_sequence/1

BBa_K1486010
type: ComponentDefinition
persistent identity: BBa K1486010
display id: BBa_K1486010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CxpR & Split IFP1.4 [Nterm + Cterm]
creator: iGem EPFL 2014
created: 2014-08-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part has two fused proteins downstream of an arabinose-inducible promoter: CpxR-IFP[1] and CpxR-IFP[2]. This construct was made in all orientations: - CpxR-IFP[1] + CpxR-IFP[2] (BBa_K1286008) - IFP[1]-CpxR + CpxR-IFP[2] (BBa_K1486010) - CpxR-IFP[1] + IFP[2]-CpxR (BBa_K1486011)
mutable notes: All orientations were made, so all dimerizations could be tested (see main page for biobrick numbers). Flexible linkers were used for each of the fused proteins, and an RBS was put before each one of them.
mutable provenance: From Michnick Article. To Edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1486000
component: BBa_B0034
component: BBa_I0500
component: BBa_K1486006
component: BBa_K1486007
component: BBa_K1486004
sequence annotation: BBa_K1486004
sequence annotation: BBa_B0034
sequence annotation: BBa_K1486000
sequence annotation: BBa_I0500
sequence annotation: BBa_K1486007
sequence annotation: BBa_K1486006
sequence: https://synbiohub.org/public/igem/BBa_K1486010_sequence/1

BBa_K1486000
type: Component
persistent identity: component2424995
display id: component2424995
version: 1
top level: BBa_K1486010
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_B0034
type: Component
persistent identity: component2424997
display id: component2424997
version: 1
top level: BBa_K1486010
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I0500
type: Component
persistent identity: component2424984
display id: component2424984
version: 1
top level: BBa_K1486010
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_K1486006
type: Component
persistent identity: component2424990
display id: component2424990
version: 1
top level: BBa_K1486010
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486006/1

BBa_K1486007
type: Component
persistent identity: component2425006
display id: component2425006
version: 1
top level: BBa_K1486010
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486007/1

BBa_K1486004
type: Component
persistent identity: component2425002
display id: component2425002
version: 1
top level: BBa_K1486010
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_K1486004
type: Component
persistent identity: component2424991
display id: component2424991
version: 1
top level: BBa_K1486010
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_B0034
type: Component
persistent identity: component2424986
display id: component2424986
version: 1
top level: BBa_K1486010
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486000
type: Component
persistent identity: component2425001
display id: component2425001
version: 1
top level: BBa_K1486010
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2424991
display id: annotation2424991
version: 1
top level: BBa_K1486010
location: https://synbiohub.org/public/igem/BBa_K1486010/annotation2424991/range2424991/1
component: BBa_K1486004

https://synbiohub.org/public/igem/BBa_K1486010/annotation2424991/range2424991/1
type: Range
persistent identity: range2424991
display id: range2424991
version: 1
direction: direction/reverse
top level: BBa_K1486010
start: 1628
end: 1657
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2424986
display id: annotation2424986
version: 1
top level: BBa_K1486010
location: https://synbiohub.org/public/igem/BBa_K1486010/annotation2424986/range2424986/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486010/annotation2424986/range2424986/1
type: Range
persistent identity: range2424986
display id: range2424986
version: 1
direction: direction/reverse
top level: BBa_K1486010
start: 1211
end: 1222
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2425002
display id: annotation2425002
version: 1
top level: BBa_K1486010
location: https://synbiohub.org/public/igem/BBa_K1486010/annotation2425002/range2425002/1
component: BBa_K1486004

https://synbiohub.org/public/igem/BBa_K1486010/annotation2425002/range2425002/1
type: Range
persistent identity: range2425002
display id: range2425002
version: 1
direction: direction/reverse
top level: BBa_K1486010
start: 3074
end: 3103
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2424997
display id: annotation2424997
version: 1
top level: BBa_K1486010
location: https://synbiohub.org/public/igem/BBa_K1486010/annotation2424997/range2424997/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486010/annotation2424997/range2424997/1
type: Range
persistent identity: range2424997
display id: range2424997
version: 1
direction: direction/reverse
top level: BBa_K1486010
start: 2360
end: 2371
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2424995
display id: annotation2424995
version: 1
top level: BBa_K1486010
location: https://synbiohub.org/public/igem/BBa_K1486010/annotation2424995/range2424995/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486010/annotation2424995/range2424995/1
type: Range
persistent identity: range2424995
display id: range2424995
version: 1
direction: direction/reverse
top level: BBa_K1486010
start: 1658
end: 2359
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2425001
display id: annotation2425001
version: 1
top level: BBa_K1486010
location: https://synbiohub.org/public/igem/BBa_K1486010/annotation2425001/range2425001/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486010/annotation2425001/range2425001/1
type: Range
persistent identity: range2425001
display id: range2425001
version: 1
direction: direction/reverse
top level: BBa_K1486010
start: 2372
end: 3073
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2424984
display id: annotation2424984
version: 1
top level: BBa_K1486010
location: https://synbiohub.org/public/igem/BBa_K1486010/annotation2424984/range2424984/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486010/annotation2424984/range2424984/1
type: Range
persistent identity: range2424984
display id: range2424984
version: 1
direction: direction/reverse
top level: BBa_K1486010
start: 1
end: 1210
orientation: inline

BBa_K1486007
type: SequenceAnnotation
persistent identity: annotation2425006
display id: annotation2425006
version: 1
top level: BBa_K1486010
location: https://synbiohub.org/public/igem/BBa_K1486010/annotation2425006/range2425006/1
component: BBa_K1486007

https://synbiohub.org/public/igem/BBa_K1486010/annotation2425006/range2425006/1
type: Range
persistent identity: range2425006
display id: range2425006
version: 1
direction: direction/reverse
top level: BBa_K1486010
start: 3104
end: 3688
orientation: inline

BBa_K1486006
type: SequenceAnnotation
persistent identity: annotation2424990
display id: annotation2424990
version: 1
top level: BBa_K1486010
location: https://synbiohub.org/public/igem/BBa_K1486010/annotation2424990/range2424990/1
component: BBa_K1486006

https://synbiohub.org/public/igem/BBa_K1486010/annotation2424990/range2424990/1
type: Range
persistent identity: range2424990
display id: range2424990
version: 1
direction: direction/reverse
top level: BBa_K1486010
start: 1223
end: 1627
orientation: inline

BBa_I732452
type: ComponentDefinition
persistent identity: BBa I732452
display id: BBa_I732452
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U073O26+D062O61)
creator: Zhan Jian
created: 2007-10-16T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732452_sequence/1

BBa_J64981
type: ComponentDefinition
persistent identity: BBa J64981
display id: BBa_J64981
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: OmpR-P strong binding, regulatory region for Team Challenge03-2007
creator: Sai Duriseti
created: 2007-03-25T11:00:00Z
modified: 2015-05-08T01:08:18Z
discontinued: false
dominant: false
group u list: _98_
m user id: 0
owner id: 1428
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Contains the F1 consensus site from the OmpF regulator. Contains a LacI repressor consensus binding site between the -35 and -10 portions of a T7 core promoter region, and a TetR consensus binding site upstream of the -35 site.
mutable notes: The weaker binding repressor binds upstream of the core promoter (upstream of -35), while the stronger binds inbetween the -35 and -10.
mutable provenance: Information for creation of this construct came from the following sorces: Yoshida et al. JBC 2006 Frank et al. JMB 1997 Rong et al. PNAS 1998 Merabet et al. Biochemistry 1995 Su et al. JBC 1998 Kedracka-Krok et al. JPC 1999 Kleinschmidt et al. Biochemistry 1988 Butler et al. JBC 1982 Jorgensen et al. JBC 1991 Hillen et al. JMB 1984 Head et al. JMB 1998
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: T7 RNA Polymerase Recognition
sequence annotation: LacI Consensus
sequence annotation: TetR Consensus
sequence annotation: Strong OmpR
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_J64981_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation1921603
display id: annotation1921603
version: 1
top level: BBa_J64981
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J64981/annotation1921603/range1921603/1

https://synbiohub.org/public/igem/BBa_J64981/annotation1921603/range1921603/1
type: Range
persistent identity: range1921603
display id: range1921603
version: 1
direction: direction/forward
top level: BBa_J64981
start: 66
end: 70
orientation: inline

T7 RNA Polymerase Recognition
type: SequenceAnnotation
persistent identity: annotation1921600
display id: annotation1921600
version: 1
top level: BBa_J64981
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J64981/annotation1921600/range1921600/1

https://synbiohub.org/public/igem/BBa_J64981/annotation1921600/range1921600/1
type: Range
persistent identity: range1921600
display id: range1921600
version: 1
direction: direction/forward
top level: BBa_J64981
start: 40
end: 82
orientation: inline

LacI Consensus
type: SequenceAnnotation
persistent identity: annotation1921601
display id: annotation1921601
version: 1
top level: BBa_J64981
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J64981/annotation1921601/range1921601/1

https://synbiohub.org/public/igem/BBa_J64981/annotation1921601/range1921601/1
type: Range
persistent identity: range1921601
display id: range1921601
version: 1
direction: direction/forward
top level: BBa_J64981
start: 46
end: 65
orientation: inline

TetR Consensus
type: SequenceAnnotation
persistent identity: annotation1921599
display id: annotation1921599
version: 1
top level: BBa_J64981
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J64981/annotation1921599/range1921599/1

https://synbiohub.org/public/igem/BBa_J64981/annotation1921599/range1921599/1
type: Range
persistent identity: range1921599
display id: range1921599
version: 1
direction: direction/forward
top level: BBa_J64981
start: 21
end: 39
orientation: inline

Strong OmpR
type: SequenceAnnotation
persistent identity: annotation1921598
display id: annotation1921598
version: 1
top level: BBa_J64981
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J64981/annotation1921598/range1921598/1

https://synbiohub.org/public/igem/BBa_J64981/annotation1921598/range1921598/1
type: Range
persistent identity: range1921598
display id: range1921598
version: 1
direction: direction/forward
top level: BBa_J64981
start: 1
end: 20
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1921602
display id: annotation1921602
version: 1
top level: BBa_J64981
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J64981/annotation1921602/range1921602/1

https://synbiohub.org/public/igem/BBa_J64981/annotation1921602/range1921602/1
type: Range
persistent identity: range1921602
display id: range1921602
version: 1
direction: direction/forward
top level: BBa_J64981
start: 40
end: 45
orientation: inline

BBa_K1486056
type: ComponentDefinition
persistent identity: BBa K1486056
display id: BBa_K1486056
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CxpR & Split IFP1.4 [Cterm + Cterm][2]
creator: iGem EPFL 2014
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is the twin of the non-compatible biobrick BBa_K1486008 rendered compatible by mutating the two illegal restriction sites. However, all experiments were run on the aforementioned biobrick.
mutable notes: Site-directed mutagenesis was used to remove the restriction sites in the sequence.
mutable provenance: http://www.addgene.org/browse/article/8177/ which was then mutated by us.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_I0500
component: BBa_K1486000
component: BBa_B0034
component: BBa_K1486004
component: BBa_K1486045
component: BBa_K1486044
sequence annotation: BBa_K1486045
sequence annotation: BBa_K1486004
sequence annotation: BBa_K1486044
sequence annotation: BBa_K1486000
sequence annotation: BBa_I0500
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1486056_sequence/1

BBa_I0500
type: Component
persistent identity: component2398599
display id: component2398599
version: 1
top level: BBa_K1486056
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_K1486000
type: Component
persistent identity: component2398605
display id: component2398605
version: 1
top level: BBa_K1486056
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_B0034
type: Component
persistent identity: component2398601
display id: component2398601
version: 1
top level: BBa_K1486056
access: public
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BBa_K1486009
type: ComponentDefinition
persistent identity: BBa K1486009
display id: BBa_K1486009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CxpR & Split IFP1.4 [Nterm + Nterm]
creator: iGem EPFL 2014
created: 2014-08-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part has two fused proteins downstream of an arabinose-inducible promoter: CpxR-IFP[1] and CpxR-IFP[2]. This construct was made in all orientations: - CpxR-IFP[1] + CpxR-IFP[2] (BBa_K1286008) - IFP[1]-CpxR + CpxR-IFP[2] (BBa_K1486010) - CpxR-IFP[1] + IFP[2]-CpxR (BBa_K1486011)
mutable notes: All orientations were made, so all dimerizations could be tested (see main page for biobrick numbers). Flexible linkers were used for each of the fused proteins, and an RBS was put before each one of them.
mutable provenance: From Michnick Article. To Edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1486007
component: BBa_I0500
component: BBa_K1486004
component: BBa_K1486000
component: BBa_K1486052
component: BBa_B0034
component: BBa_K1486006
sequence annotation: BBa_I0500
sequence annotation: BBa_K1486004
sequence annotation: BBa_B0034
sequence annotation: BBa_K1486000
sequence annotation: BBa_K1486007
sequence annotation: BBa_K1486006
sequence annotation: BBa_K1486052
sequence: https://synbiohub.org/public/igem/BBa_K1486009_sequence/1

BBa_K1486007
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BBa_I0500
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BBa_K1486004
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display id: component2420784
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BBa_K1486000
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definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_K1486052
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display id: component2420777
version: 1
top level: BBa_K1486009
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486052/1

BBa_B0034
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BBa_K1486000
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definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_B0034
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persistent identity: component2420767
display id: component2420767
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BBa_K1486000
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BBa_K1486004
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display id: component2420771
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_K1486006/1

BBa_K1486004
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persistent identity: component2420772
display id: component2420772
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_K1486007
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display id: annotation2420763
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start: 2995
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persistent identity: range2420788
display id: range2420788
version: 1
direction: direction/reverse
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start: 3025
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persistent identity: annotation2420776
display id: annotation2420776
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BBa_K1486052
type: SequenceAnnotation
persistent identity: annotation2420777
display id: annotation2420777
version: 1
top level: BBa_K1486009
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BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2420784
display id: annotation2420784
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420784/range2420784/1
component: BBa_K1486004

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persistent identity: range2420784
display id: range2420784
version: 1
direction: direction/reverse
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BBa_I739106
type: ComponentDefinition
persistent identity: BBa I739106
display id: BBa_I739106
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Double Promoter (TetR, negative / P22 cII, negative)
creator: Stefan Luzi
created: 2007-10-15T11:00:00Z
modified: 2015-08-31T04:08:01Z
discontinued: false
dominant: false
group u list: _124_
m user id: 0
owner id: 2183
owning group id: 62
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: asdf
mutable notes: asdf
mutable provenance: Synthetic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OR1
sequence annotation: R0053 (2x OR1)
sequence annotation: R0040
sequence: https://synbiohub.org/public/igem/BBa_I739106_sequence/1

OR1
type: SequenceAnnotation
persistent identity: annotation1947940
display id: annotation1947940
version: 1
top level: BBa_I739106
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I739106/annotation1947940/range1947940/1

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type: Range
persistent identity: range1947940
display id: range1947940
version: 1
direction: direction/forward
top level: BBa_I739106
start: 67
end: 84
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation1947939
display id: annotation1947939
version: 1
top level: BBa_I739106
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I739106/annotation1947939/range1947939/1

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type: Range
persistent identity: range1947939
display id: range1947939
version: 1
direction: direction/forward
top level: BBa_I739106
start: 49
end: 66
orientation: inline

R0053 (2x OR1)
type: SequenceAnnotation
persistent identity: annotation1947941
display id: annotation1947941
version: 1
top level: BBa_I739106
role: SO:0000057
role: feature/operator
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persistent identity: range1947941
display id: range1947941
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direction: direction/forward
top level: BBa_I739106
start: 49
end: 84
orientation: inline

R0040
type: SequenceAnnotation
persistent identity: annotation1947938
display id: annotation1947938
version: 1
top level: BBa_I739106
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I739106/annotation1947938/range1947938/1

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type: Range
persistent identity: range1947938
display id: range1947938
version: 1
direction: direction/forward
top level: BBa_I739106
start: 1
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orientation: inline

BBa_K091101
type: ComponentDefinition
persistent identity: BBa K091101
display id: BBa_K091101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pTet_Lac hybrid promoter
creator: Erin Feeney
created: 2008-05-28T11:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _191_
m user id: 0
owner id: 3067
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This promoter is repressed by tetR and lacI; it contains two binding sites for TetR and one LacI binding site. this part is an AND-gate since transcription occurs only in the presence of both ATC and IPTG.
mutable notes: Also designed by Pallavi Penumetcha and Robert Cool.
mutable provenance: This part consists of the R0040 tetR promoter with the addition of the R0010 lacI binding site.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: lacI binding site
sequence annotation: tetR binding site 1
sequence annotation: tetR binding site 2
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_K091101_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation1963340
display id: annotation1963340
version: 1
top level: BBa_K091101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K091101/annotation1963340/range1963340/1

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type: Range
persistent identity: range1963340
display id: range1963340
version: 1
direction: direction/forward
top level: BBa_K091101
start: 43
end: 48
orientation: inline

lacI binding site
type: SequenceAnnotation
persistent identity: annotation1963341
display id: annotation1963341
version: 1
top level: BBa_K091101
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K091101/annotation1963341/range1963341/1

https://synbiohub.org/public/igem/BBa_K091101/annotation1963341/range1963341/1
type: Range
persistent identity: range1963341
display id: range1963341
version: 1
direction: direction/forward
top level: BBa_K091101
start: 49
end: 83
orientation: inline

tetR binding site 1
type: SequenceAnnotation
persistent identity: annotation1963337
display id: annotation1963337
version: 1
top level: BBa_K091101
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K091101/annotation1963337/range1963337/1

https://synbiohub.org/public/igem/BBa_K091101/annotation1963337/range1963337/1
type: Range
persistent identity: range1963337
display id: range1963337
version: 1
direction: direction/forward
top level: BBa_K091101
start: 1
end: 19
orientation: inline

tetR binding site 2
type: SequenceAnnotation
persistent identity: annotation1963338
display id: annotation1963338
version: 1
top level: BBa_K091101
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K091101/annotation1963338/range1963338/1

https://synbiohub.org/public/igem/BBa_K091101/annotation1963338/range1963338/1
type: Range
persistent identity: range1963338
display id: range1963338
version: 1
direction: direction/forward
top level: BBa_K091101
start: 26
end: 44
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1963339
display id: annotation1963339
version: 1
top level: BBa_K091101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K091101/annotation1963339/range1963339/1

https://synbiohub.org/public/igem/BBa_K091101/annotation1963339/range1963339/1
type: Range
persistent identity: range1963339
display id: range1963339
version: 1
direction: direction/forward
top level: BBa_K091101
start: 20
end: 25
orientation: inline

BBa_K112118
type: ComponentDefinition
persistent identity: BBa K112118
display id: BBa_K112118
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rrnB P1 promoter
creator: Bing Xia
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:14Z
discontinued: false
dominant: false
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable provenance: PCR mv028/mv029 on rrnB native sequence (721bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112118 ----------------------------------------------- mv028 Forward Biobricking of rrnB P1 promoter cgataGAATTCatgAGATCTatgcactaaaacgtatcctacgcccg mv029 Reverse Biobricking of rrnB P1 promoter cgttaGGATCCcgccttcccgctacagagtcaagc
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K112118_sequence/1

U049O11+D0
type: ComponentDefinition
persistent identity: BBa I732440
display id: BBa_I732440
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U049O11+D038O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732440_sequence/1

BBa_I12036
type: ComponentDefinition
persistent identity: BBa I12036
display id: BBa_I12036
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Modified lamdba Prm promoter (cooperative repression by 434 cI)
creator: Hans
created: 2004-08-17T11:00:00Z
modified: 2015-08-31T04:07:31Z
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 103
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Lamdba Prm promoter modified to be activated by lamda repressor (cI) and repressed by 434 repressor (cI). Two operators are used for both activation and repression to enhance cooperativity.
mutable notes: The O-R3 site of lambda (17 nt) was replaced by the O-R1 site of 434 (14 nt) to facilitate repression by 434 cI. The new 434 O-R1 is 5'-justified with the lambda O-R3 it replaced, leaving the -10 sequence intact. The 434 O-R2 site replaces the wildtype labmda promoter starting at -5, thereby preserving the 8 nt between 434 O-R1 and O-R2 in the wildtype. The mRNA transcript will contain an additional 9 nt compared to wildtype lambda phage.
mutable provenance: Bushman, F. D. The bacteriophage 434 right operator roles of O-R1, O-R2, and O-R3. J. Mol. Biol. (1993) 230, 28-40.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: start
sequence annotation: OR1 lambda
sequence annotation: -10
sequence annotation: OR2 lambda
sequence annotation: -35
sequence annotation: OR1 434
sequence annotation: OR2 434
sequence: https://synbiohub.org/public/igem/BBa_I12036_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation1062203
display id: annotation1062203
version: 1
top level: BBa_I12036
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I12036/annotation1062203/range1062203/1

https://synbiohub.org/public/igem/BBa_I12036/annotation1062203/range1062203/1
type: Range
persistent identity: range1062203
display id: range1062203
version: 1
direction: direction/forward
top level: BBa_I12036
start: 83
end: 83
orientation: inline

OR1 lambda
type: SequenceAnnotation
persistent identity: annotation1056735
display id: annotation1056735
version: 1
top level: BBa_I12036
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12036/annotation1056735/range1056735/1

https://synbiohub.org/public/igem/BBa_I12036/annotation1056735/range1056735/1
type: Range
persistent identity: range1056735
display id: range1056735
version: 1
direction: direction/forward
top level: BBa_I12036
start: 9
end: 25
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1056740
display id: annotation1056740
version: 1
top level: BBa_I12036
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I12036/annotation1056740/range1056740/1

https://synbiohub.org/public/igem/BBa_I12036/annotation1056740/range1056740/1
type: Range
persistent identity: range1056740
display id: range1056740
version: 1
direction: direction/forward
top level: BBa_I12036
start: 71
end: 76
orientation: inline

OR2 lambda
type: SequenceAnnotation
persistent identity: annotation1056736
display id: annotation1056736
version: 1
top level: BBa_I12036
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12036/annotation1056736/range1056736/1

https://synbiohub.org/public/igem/BBa_I12036/annotation1056736/range1056736/1
type: Range
persistent identity: range1056736
display id: range1056736
version: 1
direction: direction/forward
top level: BBa_I12036
start: 33
end: 49
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1056739
display id: annotation1056739
version: 1
top level: BBa_I12036
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I12036/annotation1056739/range1056739/1

https://synbiohub.org/public/igem/BBa_I12036/annotation1056739/range1056739/1
type: Range
persistent identity: range1056739
display id: range1056739
version: 1
direction: direction/forward
top level: BBa_I12036
start: 48
end: 53
orientation: inline

OR1 434
type: SequenceAnnotation
persistent identity: annotation1056737
display id: annotation1056737
version: 1
top level: BBa_I12036
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12036/annotation1056737/range1056737/1

https://synbiohub.org/public/igem/BBa_I12036/annotation1056737/range1056737/1
type: Range
persistent identity: range1056737
display id: range1056737
version: 1
direction: direction/forward
top level: BBa_I12036
start: 56
end: 69
orientation: inline

OR2 434
type: SequenceAnnotation
persistent identity: annotation1056738
display id: annotation1056738
version: 1
top level: BBa_I12036
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12036/annotation1056738/range1056738/1

https://synbiohub.org/public/igem/BBa_I12036/annotation1056738/range1056738/1
type: Range
persistent identity: range1056738
display id: range1056738
version: 1
direction: direction/forward
top level: BBa_I12036
start: 78
end: 91
orientation: inline

BBa_I739105
type: ComponentDefinition
persistent identity: BBa I739105
display id: BBa_I739105
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Double Promoter (LuxR/HSL, positive / cI, negative)
creator: Stefan Luzi
created: 2007-10-14T11:00:00Z
modified: 2015-08-31T04:08:01Z
discontinued: false
dominant: false
experience: experience/None
group u list: _124_
m user id: 0
owner id: 2183
owning group id: 62
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: asd
mutable notes: asdf
mutable provenance: Synthetic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Scar
sequence annotation: OR2
sequence annotation: R0062
sequence annotation: R0051 (2x OR2)
sequence: https://synbiohub.org/public/igem/BBa_I739105_sequence/1

Scar
type: SequenceAnnotation
persistent identity: annotation1947915
display id: annotation1947915
version: 1
top level: BBa_I739105
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I739105/annotation1947915/range1947915/1

https://synbiohub.org/public/igem/BBa_I739105/annotation1947915/range1947915/1
type: Range
persistent identity: range1947915
display id: range1947915
version: 1
direction: direction/forward
top level: BBa_I739105
start: 56
end: 63
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation1947916
display id: annotation1947916
version: 1
top level: BBa_I739105
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I739105/annotation1947916/range1947916/1

https://synbiohub.org/public/igem/BBa_I739105/annotation1947916/range1947916/1
type: Range
persistent identity: range1947916
display id: range1947916
version: 1
direction: direction/forward
top level: BBa_I739105
start: 64
end: 81
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation1947917
display id: annotation1947917
version: 1
top level: BBa_I739105
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I739105/annotation1947917/range1947917/1

https://synbiohub.org/public/igem/BBa_I739105/annotation1947917/range1947917/1
type: Range
persistent identity: range1947917
display id: range1947917
version: 1
direction: direction/forward
top level: BBa_I739105
start: 82
end: 99
orientation: inline

R0062
type: SequenceAnnotation
persistent identity: annotation1947914
display id: annotation1947914
version: 1
top level: BBa_I739105
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I739105/annotation1947914/range1947914/1

https://synbiohub.org/public/igem/BBa_I739105/annotation1947914/range1947914/1
type: Range
persistent identity: range1947914
display id: range1947914
version: 1
direction: direction/forward
top level: BBa_I739105
start: 1
end: 55
orientation: inline

R0051 (2x OR2)
type: SequenceAnnotation
persistent identity: annotation1947918
display id: annotation1947918
version: 1
top level: BBa_I739105
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I739105/annotation1947918/range1947918/1

https://synbiohub.org/public/igem/BBa_I739105/annotation1947918/range1947918/1
type: Range
persistent identity: range1947918
display id: range1947918
version: 1
direction: direction/forward
top level: BBa_I739105
start: 64
end: 99
orientation: inline

BBa_K1486012
type: ComponentDefinition
persistent identity: BBa K1486012
display id: BBa_K1486012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CpxR-IFP[1] under pBAD
creator: iGem EPFL 2014
created: 2014-08-31T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part has a fused protein downstream of an arabinose-inducible promoter: CpxR-IFP[1]. Biobrick BBa_K1486013 has CpxR-IFP[2] with an arabinose promoter. This construct was made in both orientations: BBa_K1486014 is IFP[1]-CpxR (with an arabinose promoter).
mutable notes: Both orientations were made, as well as for IFP[2] so all dimerizations could be tested (see main page for biobrick numbers). Flexible linkers were used to fuse both proteins, and an RBS was put before each one of them.
mutable provenance: To Edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1486006
component: BBa_B0034
component: BBa_K1486004
component: BBa_K1486000
component: BBa_I0500
sequence annotation: BBa_I0500
sequence annotation: BBa_K1486006
sequence annotation: BBa_K1486004
sequence annotation: BBa_K1486000
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1486012_sequence/1

BBa_K1486006
type: Component
persistent identity: component2382293
display id: component2382293
version: 1
top level: BBa_K1486012
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486006/1

BBa_B0034
type: Component
persistent identity: component2382287
display id: component2382287
version: 1
top level: BBa_K1486012
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486004
type: Component
persistent identity: component2382292
display id: component2382292
version: 1
top level: BBa_K1486012
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_K1486000
type: Component
persistent identity: component2382291
display id: component2382291
version: 1
top level: BBa_K1486012
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_I0500
type: Component
persistent identity: component2382285
display id: component2382285
version: 1
top level: BBa_K1486012
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2382285
display id: annotation2382285
version: 1
top level: BBa_K1486012
location: https://synbiohub.org/public/igem/BBa_K1486012/annotation2382285/range2382285/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486012/annotation2382285/range2382285/1
type: Range
persistent identity: range2382285
display id: range2382285
version: 1
direction: direction/reverse
top level: BBa_K1486012
start: 1
end: 1210
orientation: inline

BBa_K1486006
type: SequenceAnnotation
persistent identity: annotation2382293
display id: annotation2382293
version: 1
top level: BBa_K1486012
location: https://synbiohub.org/public/igem/BBa_K1486012/annotation2382293/range2382293/1
component: BBa_K1486006

https://synbiohub.org/public/igem/BBa_K1486012/annotation2382293/range2382293/1
type: Range
persistent identity: range2382293
display id: range2382293
version: 1
direction: direction/reverse
top level: BBa_K1486012
start: 1955
end: 2536
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2382292
display id: annotation2382292
version: 1
top level: BBa_K1486012
location: https://synbiohub.org/public/igem/BBa_K1486012/annotation2382292/range2382292/1
component: BBa_K1486004

https://synbiohub.org/public/igem/BBa_K1486012/annotation2382292/range2382292/1
type: Range
persistent identity: range2382292
display id: range2382292
version: 1
direction: direction/reverse
top level: BBa_K1486012
start: 1925
end: 1954
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2382291
display id: annotation2382291
version: 1
top level: BBa_K1486012
location: https://synbiohub.org/public/igem/BBa_K1486012/annotation2382291/range2382291/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486012/annotation2382291/range2382291/1
type: Range
persistent identity: range2382291
display id: range2382291
version: 1
direction: direction/reverse
top level: BBa_K1486012
start: 1223
end: 1924
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2382287
display id: annotation2382287
version: 1
top level: BBa_K1486012
location: https://synbiohub.org/public/igem/BBa_K1486012/annotation2382287/range2382287/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486012/annotation2382287/range2382287/1
type: Range
persistent identity: range2382287
display id: range2382287
version: 1
direction: direction/reverse
top level: BBa_K1486012
start: 1211
end: 1222
orientation: inline

BBa_K165041
type: ComponentDefinition
persistent identity: BBa K165041
display id: BBa_K165041
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Zif268-HIV binding sites + TEF constitutive yeast promoter
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K165011
component: BBa_K165037
sequence annotation: BBa_K165037
sequence annotation: BBa_K165011
sequence: https://synbiohub.org/public/igem/BBa_K165041_sequence/1

BBa_K165011
type: Component
persistent identity: component1996557
display id: component1996557
version: 1
top level: BBa_K165041
access: public
definition: https://synbiohub.org/public/igem/BBa_K165011/1

BBa_K165037
type: Component
persistent identity: component1996558
display id: component1996558
version: 1
top level: BBa_K165041
access: public
definition: https://synbiohub.org/public/igem/BBa_K165037/1

BBa_K165037
type: SequenceAnnotation
persistent identity: annotation1996558
display id: annotation1996558
version: 1
top level: BBa_K165041
location: https://synbiohub.org/public/igem/BBa_K165041/annotation1996558/range1996558/1
component: BBa_K165037

https://synbiohub.org/public/igem/BBa_K165041/annotation1996558/range1996558/1
type: Range
persistent identity: range1996558
display id: range1996558
version: 1
direction: direction/reverse
top level: BBa_K165041
start: 55
end: 457
orientation: inline

BBa_K165011
type: SequenceAnnotation
persistent identity: annotation1996557
display id: annotation1996557
version: 1
top level: BBa_K165041
location: https://synbiohub.org/public/igem/BBa_K165041/annotation1996557/range1996557/1
component: BBa_K165011

https://synbiohub.org/public/igem/BBa_K165041/annotation1996557/range1996557/1
type: Range
persistent identity: range1996557
display id: range1996557
version: 1
direction: direction/reverse
top level: BBa_K165041
start: 1
end: 46
orientation: inline

BBa_K125100
type: ComponentDefinition
persistent identity: BBa K125100
display id: BBa_K125100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: nir promoter from Synechocystis sp. PCC6803
creator: Krystle Salazar and Grace Kwan
created: 2008-07-23T11:00:00Z
modified: 2015-05-08T01:09:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _
m user id: 0
owner id: 3229
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The ''nir'' promoter is nitrate inducible.
mutable provenance: The ''nirA'' promoter originates from ''Synechocystis'' sp. PCC6803.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Ntc-A binding motif
sequence annotation: Ntc-B binding motif
sequence annotation: -10 sequence
sequence annotation: nir promoter
sequence: https://synbiohub.org/public/igem/BBa_K125100_sequence/1

Ntc-A binding motif
type: SequenceAnnotation
persistent identity: annotation1968489
display id: annotation1968489
version: 1
top level: BBa_K125100
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K125100/annotation1968489/range1968489/1

https://synbiohub.org/public/igem/BBa_K125100/annotation1968489/range1968489/1
type: Range
persistent identity: range1968489
display id: range1968489
version: 1
direction: direction/forward
top level: BBa_K125100
start: 36
end: 44
orientation: inline

Ntc-B binding motif
type: SequenceAnnotation
persistent identity: annotation1968488
display id: annotation1968488
version: 1
top level: BBa_K125100
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K125100/annotation1968488/range1968488/1

https://synbiohub.org/public/igem/BBa_K125100/annotation1968488/range1968488/1
type: Range
persistent identity: range1968488
display id: range1968488
version: 1
direction: direction/forward
top level: BBa_K125100
start: 5
end: 20
orientation: inline

-10 sequence
type: SequenceAnnotation
persistent identity: annotation1968487
display id: annotation1968487
version: 1
top level: BBa_K125100
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K125100/annotation1968487/range1968487/1

https://synbiohub.org/public/igem/BBa_K125100/annotation1968487/range1968487/1
type: Range
persistent identity: range1968487
display id: range1968487
version: 1
direction: direction/forward
top level: BBa_K125100
start: 73
end: 78
orientation: inline

nir promoter
type: SequenceAnnotation
persistent identity: annotation1968486
display id: annotation1968486
version: 1
top level: BBa_K125100
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K125100/annotation1968486/range1968486/1

https://synbiohub.org/public/igem/BBa_K125100/annotation1968486/range1968486/1
type: Range
persistent identity: range1968486
display id: range1968486
version: 1
direction: direction/forward
top level: BBa_K125100
start: 1
end: 88
orientation: inline

U085O11+D0
type: ComponentDefinition
persistent identity: BBa I732424
display id: BBa_I732424
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U085O11+D062O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732424_sequence/1

U097NUL+D0
type: ComponentDefinition
persistent identity: BBa I732408
display id: BBa_I732408
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U097NUL+D014O22)
creator: Zhan Jian
created: 2007-09-19T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1572
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: A Double Reporter (LacZ-alpha and RFP) loaded on pSB3C5. For more information please refer to BBa_I732402.
mutable notes: Might be later edited.
mutable provenance: Might be later edited.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BglII
sequence annotation: conserved
sequence annotation: polya
sequence annotation: mrfp1
sequence annotation: lacZ
sequence annotation: stem_loop
sequence annotation: BBa_B0034
sequence annotation: T7 TE
sequence annotation: STOP
sequence annotation: BBa_B0012
sequence annotation: BBa_E1010
sequence annotation: BBa_I732007
sequence annotation: stop
sequence annotation: barcode
sequence annotation: BBa_B0010
sequence annotation: BBa_I732006
sequence annotation: BamHI
sequence annotation: START
sequence: https://synbiohub.org/public/igem/BBa_I732408_sequence/1

BglII
type: SequenceAnnotation
persistent identity: annotation1944497
display id: annotation1944497
version: 1
top level: U097NUL+D0
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944497/range1944497/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944497/range1944497/1
type: Range
persistent identity: range1944497
display id: range1944497
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 3
end: 8
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation1944500
display id: annotation1944500
version: 1
top level: U097NUL+D0
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944500/range1944500/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944500/range1944500/1
type: Range
persistent identity: range1944500
display id: range1944500
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 32
end: 35
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1944515
display id: annotation1944515
version: 1
top level: U097NUL+D0
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944515/range1944515/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944515/range1944515/1
type: Range
persistent identity: range1944515
display id: range1944515
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 1135
end: 1148
orientation: inline

mrfp1
type: SequenceAnnotation
persistent identity: annotation1944508
display id: annotation1944508
version: 1
top level: U097NUL+D0
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944508/range1944508/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944508/range1944508/1
type: Range
persistent identity: range1944508
display id: range1944508
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 306
end: 980
orientation: inline

lacZ
type: SequenceAnnotation
persistent identity: annotation1944503
display id: annotation1944503
version: 1
top level: U097NUL+D0
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944503/range1944503/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944503/range1944503/1
type: Range
persistent identity: range1944503
display id: range1944503
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 46
end: 279
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1944512
display id: annotation1944512
version: 1
top level: U097NUL+D0
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944512/range1944512/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944512/range1944512/1
type: Range
persistent identity: range1944512
display id: range1944512
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 1031
end: 1074
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1944507
display id: annotation1944507
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944507/range1944507/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944507/range1944507/1
type: Range
persistent identity: range1944507
display id: range1944507
version: 1
direction: direction/reverse
top level: U097NUL+D0
start: 288
end: 299
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1944514
display id: annotation1944514
version: 1
top level: U097NUL+D0
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944514/range1944514/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944514/range1944514/1
type: Range
persistent identity: range1944514
display id: range1944514
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 1115
end: 1134
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation1944504
display id: annotation1944504
version: 1
top level: U097NUL+D0
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944504/range1944504/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944504/range1944504/1
type: Range
persistent identity: range1944504
display id: range1944504
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 274
end: 279
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1944513
display id: annotation1944513
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944513/range1944513/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944513/range1944513/1
type: Range
persistent identity: range1944513
display id: range1944513
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 1108
end: 1148
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1944509
display id: annotation1944509
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944509/range1944509/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944509/range1944509/1
type: Range
persistent identity: range1944509
display id: range1944509
version: 1
direction: direction/reverse
top level: U097NUL+D0
start: 306
end: 986
orientation: inline

BBa_I732007
type: SequenceAnnotation
persistent identity: annotation1944499
display id: annotation1944499
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944499/range1944499/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944499/range1944499/1
type: Range
persistent identity: range1944499
display id: range1944499
version: 1
direction: direction/reverse
top level: U097NUL+D0
start: 1
end: 19
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1944516
display id: annotation1944516
version: 1
top level: U097NUL+D0
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944516/range1944516/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944516/range1944516/1
type: Range
persistent identity: range1944516
display id: range1944516
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 1141
end: 1141
orientation: inline

barcode
type: SequenceAnnotation
persistent identity: annotation1944510
display id: annotation1944510
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944510/range1944510/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944510/range1944510/1
type: Range
persistent identity: range1944510
display id: range1944510
version: 1
direction: direction/reverse
top level: U097NUL+D0
start: 987
end: 1011
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1944511
display id: annotation1944511
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944511/range1944511/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944511/range1944511/1
type: Range
persistent identity: range1944511
display id: range1944511
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 1020
end: 1099
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation1944506
display id: annotation1944506
version: 1
top level: U097NUL+D0
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944506/range1944506/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944506/range1944506/1
type: Range
persistent identity: range1944506
display id: range1944506
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 292
end: 295
orientation: inline

BBa_I732006
type: SequenceAnnotation
persistent identity: annotation1944505
display id: annotation1944505
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944505/range1944505/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944505/range1944505/1
type: Range
persistent identity: range1944505
display id: range1944505
version: 1
direction: direction/reverse
top level: U097NUL+D0
start: 46
end: 279
orientation: inline

BamHI
type: SequenceAnnotation
persistent identity: annotation1944498
display id: annotation1944498
version: 1
top level: U097NUL+D0
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944498/range1944498/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944498/range1944498/1
type: Range
persistent identity: range1944498
display id: range1944498
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 12
end: 17
orientation: inline

START
type: SequenceAnnotation
persistent identity: annotation1944502
display id: annotation1944502
version: 1
top level: U097NUL+D0
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944502/range1944502/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944502/range1944502/1
type: Range
persistent identity: range1944502
display id: range1944502
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 46
end: 48
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1944501
display id: annotation1944501
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944501/range1944501/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944501/range1944501/1
type: Range
persistent identity: range1944501
display id: range1944501
version: 1
direction: direction/reverse
top level: U097NUL+D0
start: 28
end: 39
orientation: inline

BBa_K1486005
type: ComponentDefinition
persistent identity: BBa K1486005
display id: BBa_K1486005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Arabinose promoter + sfGFP-CpxR [Cterm]
creator: iGem EPFL 2014
created: 2014-08-12T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is composed of an arabinose-inducible promoter for superfolded GFP attached by a linker to CpxR (on the C terminus of CpxR).
mutable notes: We had to test both attachments, N and C terminus of the CpxR.
mutable provenance: The arabinose promoter and the sfGFP were obtained from the registry, while the CpxR was obtained from the E coli genome.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_I0500
component: BBa_I746916
component: BBa_K1486000
component: BBa_K1486003
sequence annotation: BBa_I0500
sequence annotation: BBa_K1486003
sequence annotation: BBa_B0034
sequence annotation: BBa_K1486000
sequence annotation: BBa_I746916
sequence: https://synbiohub.org/public/igem/BBa_K1486005_sequence/1

BBa_B0034
type: Component
persistent identity: component2382084
display id: component2382084
version: 1
top level: BBa_K1486005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I0500
type: Component
persistent identity: component2382082
display id: component2382082
version: 1
top level: BBa_K1486005
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_I746916
type: Component
persistent identity: component2382093
display id: component2382093
version: 1
top level: BBa_K1486005
access: public
definition: https://synbiohub.org/public/igem/BBa_I746916/1

BBa_K1486000
type: Component
persistent identity: component2382088
display id: component2382088
version: 1
top level: BBa_K1486005
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_K1486003
type: Component
persistent identity: component2382089
display id: component2382089
version: 1
top level: BBa_K1486005
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486003/1

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2382082
display id: annotation2382082
version: 1
top level: BBa_K1486005
location: https://synbiohub.org/public/igem/BBa_K1486005/annotation2382082/range2382082/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486005/annotation2382082/range2382082/1
type: Range
persistent identity: range2382082
display id: range2382082
version: 1
direction: direction/reverse
top level: BBa_K1486005
start: 1
end: 1210
orientation: inline

BBa_K1486003
type: SequenceAnnotation
persistent identity: annotation2382089
display id: annotation2382089
version: 1
top level: BBa_K1486005
location: https://synbiohub.org/public/igem/BBa_K1486005/annotation2382089/range2382089/1
component: BBa_K1486003

https://synbiohub.org/public/igem/BBa_K1486005/annotation2382089/range2382089/1
type: Range
persistent identity: range2382089
display id: range2382089
version: 1
direction: direction/reverse
top level: BBa_K1486005
start: 1925
end: 1948
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2382084
display id: annotation2382084
version: 1
top level: BBa_K1486005
location: https://synbiohub.org/public/igem/BBa_K1486005/annotation2382084/range2382084/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486005/annotation2382084/range2382084/1
type: Range
persistent identity: range2382084
display id: range2382084
version: 1
direction: direction/reverse
top level: BBa_K1486005
start: 1211
end: 1222
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2382088
display id: annotation2382088
version: 1
top level: BBa_K1486005
location: https://synbiohub.org/public/igem/BBa_K1486005/annotation2382088/range2382088/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486005/annotation2382088/range2382088/1
type: Range
persistent identity: range2382088
display id: range2382088
version: 1
direction: direction/reverse
top level: BBa_K1486005
start: 1223
end: 1924
orientation: inline

BBa_I746916
type: SequenceAnnotation
persistent identity: annotation2382093
display id: annotation2382093
version: 1
top level: BBa_K1486005
location: https://synbiohub.org/public/igem/BBa_K1486005/annotation2382093/range2382093/1
component: BBa_I746916

https://synbiohub.org/public/igem/BBa_K1486005/annotation2382093/range2382093/1
type: Range
persistent identity: range2382093
display id: range2382093
version: 1
direction: direction/reverse
top level: BBa_K1486005
start: 1949
end: 2668
orientation: inline

BBa_I739107
type: ComponentDefinition
persistent identity: BBa I739107
display id: BBa_I739107
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Double Promoter (cI, negative / LacI, negative)
creator: Stefan Luzi
created: 2007-10-16T11:00:00Z
modified: 2015-08-31T04:08:01Z
discontinued: false
dominant: false
group u list: _124_
m user id: 0
owner id: 2183
owning group id: 62
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: asdf
mutable notes: sadf
mutable provenance: Synthetic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: R0010
sequence annotation: LacI binding site
sequence annotation: R0051
sequence: https://synbiohub.org/public/igem/BBa_I739107_sequence/1

R0010
type: SequenceAnnotation
persistent identity: annotation1948512
display id: annotation1948512
version: 1
top level: BBa_I739107
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I739107/annotation1948512/range1948512/1

https://synbiohub.org/public/igem/BBa_I739107/annotation1948512/range1948512/1
type: Range
persistent identity: range1948512
display id: range1948512
version: 1
direction: direction/forward
top level: BBa_I739107
start: 44
end: 78
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1948511
display id: annotation1948511
version: 1
top level: BBa_I739107
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I739107/annotation1948511/range1948511/1

https://synbiohub.org/public/igem/BBa_I739107/annotation1948511/range1948511/1
type: Range
persistent identity: range1948511
display id: range1948511
version: 1
direction: direction/forward
top level: BBa_I739107
start: 44
end: 78
orientation: inline

R0051
type: SequenceAnnotation
persistent identity: annotation1948510
display id: annotation1948510
version: 1
top level: BBa_I739107
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I739107/annotation1948510/range1948510/1

https://synbiohub.org/public/igem/BBa_I739107/annotation1948510/range1948510/1
type: Range
persistent identity: range1948510
display id: range1948510
version: 1
direction: direction/forward
top level: BBa_I739107
start: 1
end: 43
orientation: inline

BS
type: ComponentDefinition
persistent identity: BBa K566002
display id: BBa_K566002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Biphasic switch
creator: Daniel Rodriguez, Porfirio Quintero
created: 2011-09-25T11:00:00Z
modified: 2015-05-08T01:12:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _734_
m user id: 0
owner id: 8650
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: Biphasic switch is a composite part which permits positive and negative regulation of a gene set through pRM promoter and OL region from Lambda phage.
mutable notes: Distance between pRM and OL must be longer than 1.6 kb due to necessity of loop formation for more effiecient repression state of the switch.
mutable provenance: Extracted directly from Lambda genome by separate as pRM and OL parts, constructed by classic BioBricks system.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K566001
component: BBa_K566000
sequence annotation: BBa_K566000
sequence annotation: BBa_K566001
sequence: https://synbiohub.org/public/igem/BBa_K566002_sequence/1

BBa_K566001
type: Component
persistent identity: component2142163
display id: component2142163
version: 1
top level: BS
access: public
definition: pRM

BBa_K566000
type: Component
persistent identity: component2142164
display id: component2142164
version: 1
top level: BS
access: public
definition: OL

BBa_K566000
type: SequenceAnnotation
persistent identity: annotation2142164
display id: annotation2142164
version: 1
top level: BS
location: https://synbiohub.org/public/igem/BBa_K566002/annotation2142164/range2142164/1
component: BBa_K566000

https://synbiohub.org/public/igem/BBa_K566002/annotation2142164/range2142164/1
type: Range
persistent identity: range2142164
display id: range2142164
version: 1
direction: direction/reverse
top level: BS
start: 94
end: 222
orientation: inline

BBa_K566001
type: SequenceAnnotation
persistent identity: annotation2142163
display id: annotation2142163
version: 1
top level: BS
location: https://synbiohub.org/public/igem/BBa_K566002/annotation2142163/range2142163/1
component: BBa_K566001

https://synbiohub.org/public/igem/BBa_K566002/annotation2142163/range2142163/1
type: Range
persistent identity: range2142163
display id: range2142163
version: 1
direction: direction/reverse
top level: BS
start: 1
end: 85
orientation: inline

BBa_K145152
type: ComponentDefinition
persistent identity: BBa K145152
display id: BBa_K145152
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Hybrid promoter: P22 c2 , LacI NOR gate
creator: Jonas Demeulemeester
created: 2008-08-11T11:00:00Z
modified: 2015-05-08T01:10:29Z
discontinued: false
dominant: false
group u list: _257_
m user id: 0
owner id: 2970
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Hybrid promoter constisting of two LacI O2 sites at the ends of the promoter and two recognition sites (OR2 and OR1) for the P22 c2 repressor. The promoter should function as a NOR gate.
mutable notes: a small fragment of OR3 remains.
mutable provenance: synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: O1 P22 c2
sequence annotation: O2 LacI
sequence annotation: O2 P22 c2
sequence annotation: -10
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_K145152_sequence/1

O1 P22 c2
type: SequenceAnnotation
persistent identity: annotation1971587
display id: annotation1971587
version: 1
top level: BBa_K145152
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K145152/annotation1971587/range1971587/1

https://synbiohub.org/public/igem/BBa_K145152/annotation1971587/range1971587/1
type: Range
persistent identity: range1971587
display id: range1971587
version: 1
direction: direction/forward
top level: BBa_K145152
start: 82
end: 97
orientation: inline

O2 LacI
type: SequenceAnnotation
persistent identity: annotation1971579
display id: annotation1971579
version: 1
top level: BBa_K145152
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K145152/annotation1971579/range1971579/1

https://synbiohub.org/public/igem/BBa_K145152/annotation1971579/range1971579/1
type: Range
persistent identity: range1971579
display id: range1971579
version: 1
direction: direction/forward
top level: BBa_K145152
start: 1
end: 21
orientation: inline

O2 P22 c2
type: SequenceAnnotation
persistent identity: annotation1971581
display id: annotation1971581
version: 1
top level: BBa_K145152
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K145152/annotation1971581/range1971581/1

https://synbiohub.org/public/igem/BBa_K145152/annotation1971581/range1971581/1
type: Range
persistent identity: range1971581
display id: range1971581
version: 1
direction: direction/forward
top level: BBa_K145152
start: 59
end: 76
orientation: inline

O2 LacI
type: SequenceAnnotation
persistent identity: annotation1971580
display id: annotation1971580
version: 1
top level: BBa_K145152
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K145152/annotation1971580/range1971580/1

https://synbiohub.org/public/igem/BBa_K145152/annotation1971580/range1971580/1
type: Range
persistent identity: range1971580
display id: range1971580
version: 1
direction: direction/forward
top level: BBa_K145152
start: 115
end: 135
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1971558
display id: annotation1971558
version: 1
top level: BBa_K145152
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K145152/annotation1971558/range1971558/1

https://synbiohub.org/public/igem/BBa_K145152/annotation1971558/range1971558/1
type: Range
persistent identity: range1971558
display id: range1971558
version: 1
direction: direction/forward
top level: BBa_K145152
start: 78
end: 83
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1971557
display id: annotation1971557
version: 1
top level: BBa_K145152
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K145152/annotation1971557/range1971557/1

https://synbiohub.org/public/igem/BBa_K145152/annotation1971557/range1971557/1
type: Range
persistent identity: range1971557
display id: range1971557
version: 1
direction: direction/forward
top level: BBa_K145152
start: 56
end: 61
orientation: inline

BBa_I765007
type: ComponentDefinition
persistent identity: BBa I765007
display id: BBa_I765007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fe and UV promoters
creator: Claudia Guevara and Alex Quintero
created: 2007-10-25T11:00:00Z
modified: 2015-08-31T04:08:09Z
discontinued: false
dominant: false
experience: experience/None
group u list: _118_
m user id: 0
owner id: 2267
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Regulated by the presence of Iron and UV light irradiation
mutable notes: None
mutable provenance: Genomic sequence
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_I765000
component: BBa_I765001
sequence annotation: BBa_I765001
sequence annotation: BBa_I765000
sequence: https://synbiohub.org/public/igem/BBa_I765007_sequence/1

BBa_I765000
type: Component
persistent identity: component1958274
display id: component1958274
version: 1
top level: BBa_I765007
access: public
definition: https://synbiohub.org/public/igem/BBa_I765000/1

BBa_I765001
type: Component
persistent identity: component1958276
display id: component1958276
version: 1
top level: BBa_I765007
access: public
definition: https://synbiohub.org/public/igem/BBa_I765001/1

BBa_I765001
type: SequenceAnnotation
persistent identity: annotation1958276
display id: annotation1958276
version: 1
top level: BBa_I765007
location: https://synbiohub.org/public/igem/BBa_I765007/annotation1958276/range1958276/1
component: BBa_I765001

https://synbiohub.org/public/igem/BBa_I765007/annotation1958276/range1958276/1
type: Range
persistent identity: range1958276
display id: range1958276
version: 1
direction: direction/reverse
top level: BBa_I765007
start: 1053
end: 1128
orientation: inline

BBa_I765000
type: SequenceAnnotation
persistent identity: annotation1958274
display id: annotation1958274
version: 1
top level: BBa_I765007
location: https://synbiohub.org/public/igem/BBa_I765007/annotation1958274/range1958274/1
component: BBa_I765000

https://synbiohub.org/public/igem/BBa_I765007/annotation1958274/range1958274/1
type: Range
persistent identity: range1958274
display id: range1958274
version: 1
direction: direction/reverse
top level: BBa_I765007
start: 1
end: 1044
orientation: inline

U073O11+D0
type: ComponentDefinition
persistent identity: BBa I732430
display id: BBa_I732430
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U073O11+D062O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732430_sequence/1

U049O11+D0
type: ComponentDefinition
persistent identity: BBa I732437
display id: BBa_I732437
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U049O11+D002O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732437_sequence/1

BBa_K1486055
type: ComponentDefinition
persistent identity: BBa K1486055
display id: BBa_K1486055
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CheY/CheZ + split fLuc
creator: EPFL iGem team 2014
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: to edit
mutable notes: to edit
mutable provenance: to edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1486053
component: BBa_I0500
component: BBa_K1486016
component: BBa_B0034
component: BBa_K569017
component: BBa_K1486017
component: BBa_K629003
sequence annotation: BBa_K1486017
sequence annotation: BBa_K1486053
sequence annotation: BBa_B0034
sequence annotation: BBa_K629003
sequence annotation: BBa_K1486016
sequence annotation: BBa_K569017
sequence annotation: BBa_I0500
sequence: https://synbiohub.org/public/igem/BBa_K1486055_sequence/1

BBa_K1486053
type: Component
persistent identity: component2399883
display id: component2399883
version: 1
top level: BBa_K1486055
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486053/1

BBa_I0500
type: Component
persistent identity: component2399879
display id: component2399879
version: 1
top level: BBa_K1486055
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_K1486016
type: Component
persistent identity: component2399884
display id: component2399884
version: 1
top level: BBa_K1486055
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486016/1

BBa_B0034
type: Component
persistent identity: component2399881
display id: component2399881
version: 1
top level: BBa_K1486055
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K569017
type: Component
persistent identity: component2399882
display id: component2399882
version: 1
top level: BBa_K1486055
access: public
definition: https://synbiohub.org/public/igem/BBa_K569017/1

BBa_B0034
type: Component
persistent identity: component2399886
display id: component2399886
version: 1
top level: BBa_K1486055
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486017
type: Component
persistent identity: component2399895
display id: component2399895
version: 1
top level: BBa_K1486055
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486017/1

BBa_K629003
type: Component
persistent identity: component2399893
display id: component2399893
version: 1
top level: BBa_K1486055
access: public
definition: https://synbiohub.org/public/igem/BBa_K629003/1

BBa_K1486053
type: Component
persistent identity: component2399894
display id: component2399894
version: 1
top level: BBa_K1486055
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486053/1

BBa_K1486017
type: SequenceAnnotation
persistent identity: annotation2399895
display id: annotation2399895
version: 1
top level: BBa_K1486055
location: https://synbiohub.org/public/igem/BBa_K1486055/annotation2399895/range2399895/1
component: BBa_K1486017

https://synbiohub.org/public/igem/BBa_K1486055/annotation2399895/range2399895/1
type: Range
persistent identity: range2399895
display id: range2399895
version: 1
direction: direction/reverse
top level: BBa_K1486055
start: 2832
end: 4079
orientation: inline

BBa_K1486053
type: SequenceAnnotation
persistent identity: annotation2399894
display id: annotation2399894
version: 1
top level: BBa_K1486055
location: https://synbiohub.org/public/igem/BBa_K1486055/annotation2399894/range2399894/1
component: BBa_K1486053

https://synbiohub.org/public/igem/BBa_K1486055/annotation2399894/range2399894/1
type: Range
persistent identity: range2399894
display id: range2399894
version: 1
direction: direction/reverse
top level: BBa_K1486055
start: 2802
end: 2831
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2399886
display id: annotation2399886
version: 1
top level: BBa_K1486055
location: https://synbiohub.org/public/igem/BBa_K1486055/annotation2399886/range2399886/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486055/annotation2399886/range2399886/1
type: Range
persistent identity: range2399886
display id: range2399886
version: 1
direction: direction/reverse
top level: BBa_K1486055
start: 2145
end: 2156
orientation: inline

BBa_K629003
type: SequenceAnnotation
persistent identity: annotation2399893
display id: annotation2399893
version: 1
top level: BBa_K1486055
location: https://synbiohub.org/public/igem/BBa_K1486055/annotation2399893/range2399893/1
component: BBa_K629003

https://synbiohub.org/public/igem/BBa_K1486055/annotation2399893/range2399893/1
type: Range
persistent identity: range2399893
display id: range2399893
version: 1
direction: direction/reverse
top level: BBa_K1486055
start: 2157
end: 2801
orientation: inline

BBa_K1486016
type: SequenceAnnotation
persistent identity: annotation2399884
display id: annotation2399884
version: 1
top level: BBa_K1486055
location: https://synbiohub.org/public/igem/BBa_K1486055/annotation2399884/range2399884/1
component: BBa_K1486016

https://synbiohub.org/public/igem/BBa_K1486055/annotation2399884/range2399884/1
type: Range
persistent identity: range2399884
display id: range2399884
version: 1
direction: direction/reverse
top level: BBa_K1486055
start: 1671
end: 2144
orientation: inline

BBa_K569017
type: SequenceAnnotation
persistent identity: annotation2399882
display id: annotation2399882
version: 1
top level: BBa_K1486055
location: https://synbiohub.org/public/igem/BBa_K1486055/annotation2399882/range2399882/1
component: BBa_K569017

https://synbiohub.org/public/igem/BBa_K1486055/annotation2399882/range2399882/1
type: Range
persistent identity: range2399882
display id: range2399882
version: 1
direction: direction/reverse
top level: BBa_K1486055
start: 1223
end: 1640
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2399881
display id: annotation2399881
version: 1
top level: BBa_K1486055
location: https://synbiohub.org/public/igem/BBa_K1486055/annotation2399881/range2399881/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486055/annotation2399881/range2399881/1
type: Range
persistent identity: range2399881
display id: range2399881
version: 1
direction: direction/reverse
top level: BBa_K1486055
start: 1211
end: 1222
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2399879
display id: annotation2399879
version: 1
top level: BBa_K1486055
location: https://synbiohub.org/public/igem/BBa_K1486055/annotation2399879/range2399879/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486055/annotation2399879/range2399879/1
type: Range
persistent identity: range2399879
display id: range2399879
version: 1
direction: direction/reverse
top level: BBa_K1486055
start: 1
end: 1210
orientation: inline

BBa_K1486053
type: SequenceAnnotation
persistent identity: annotation2399883
display id: annotation2399883
version: 1
top level: BBa_K1486055
location: https://synbiohub.org/public/igem/BBa_K1486055/annotation2399883/range2399883/1
component: BBa_K1486053

https://synbiohub.org/public/igem/BBa_K1486055/annotation2399883/range2399883/1
type: Range
persistent identity: range2399883
display id: range2399883
version: 1
direction: direction/reverse
top level: BBa_K1486055
start: 1641
end: 1670
orientation: inline

BBa_K110016
type: ComponentDefinition
persistent identity: BBa K110016
display id: BBa_K110016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: A-Cell Promoter STE2 (backwards)
creator: James DiCarlo
created: 2008-08-26T11:00:00Z
modified: 2015-05-08T01:09:09Z
discontinued: false
dominant: false
experience: experience/None
group u list: _201_
m user id: 0
owner id: 2669
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is the same sequence as STE2 promotor(BBa_K110008), but with suffix and prefix switched
mutable notes: This is directly from the DNA sequence
mutable provenance: sgd
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: MATalpha2
sequence annotation: MCM1
sequence annotation: STE2 Promoter
sequence annotation: STE12
sequence: https://synbiohub.org/public/igem/BBa_K110016_sequence/1

MATalpha2
type: SequenceAnnotation
persistent identity: annotation1981091
display id: annotation1981091
version: 1
top level: BBa_K110016
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110016/annotation1981091/range1981091/1

https://synbiohub.org/public/igem/BBa_K110016/annotation1981091/range1981091/1
type: Range
persistent identity: range1981091
display id: range1981091
version: 1
direction: direction/forward
top level: BBa_K110016
start: 273
end: 282
orientation: inline

MCM1
type: SequenceAnnotation
persistent identity: annotation1981092
display id: annotation1981092
version: 1
top level: BBa_K110016
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110016/annotation1981092/range1981092/1

https://synbiohub.org/public/igem/BBa_K110016/annotation1981092/range1981092/1
type: Range
persistent identity: range1981092
display id: range1981092
version: 1
direction: direction/forward
top level: BBa_K110016
start: 284
end: 293
orientation: inline

STE2 Promoter
type: SequenceAnnotation
persistent identity: annotation1981094
display id: annotation1981094
version: 1
top level: BBa_K110016
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K110016/annotation1981094/range1981094/1

https://synbiohub.org/public/igem/BBa_K110016/annotation1981094/range1981094/1
type: Range
persistent identity: range1981094
display id: range1981094
version: 1
direction: direction/forward
top level: BBa_K110016
start: 1
end: 500
orientation: inline

STE12
type: SequenceAnnotation
persistent identity: annotation1981095
display id: annotation1981095
version: 1
top level: BBa_K110016
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110016/annotation1981095/range1981095/1

https://synbiohub.org/public/igem/BBa_K110016/annotation1981095/range1981095/1
type: Range
persistent identity: range1981095
display id: range1981095
version: 1
direction: direction/forward
top level: BBa_K110016
start: 304
end: 311
orientation: inline

MATalpha2
type: SequenceAnnotation
persistent identity: annotation1981093
display id: annotation1981093
version: 1
top level: BBa_K110016
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110016/annotation1981093/range1981093/1

https://synbiohub.org/public/igem/BBa_K110016/annotation1981093/range1981093/1
type: Range
persistent identity: range1981093
display id: range1981093
version: 1
direction: direction/forward
top level: BBa_K110016
start: 295
end: 302
orientation: inline

pSB1A30
type: ComponentDefinition
persistent identity: BBa K1362091
display id: BBa_K1362091
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB1A30: High copy BioBrick cloning/expression backbone carrying Amp resitance
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1738_
m user id: 0
owner id: 22830
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: terminator stem loop
sequence annotation: lacO reg
sequence annotation: VF2
sequence annotation: ampR (rev)
sequence annotation: E. coli his operon terminator
sequence annotation: prefix
sequence annotation: end of terminator
sequence annotation: ORI
sequence annotation: VR
sequence annotation: suffix
sequence annotation: T7 promotor
sequence annotation: rep(pMB1) (rev)
sequence: https://synbiohub.org/public/igem/BBa_K1362091_sequence/1

terminator stem loop
type: SequenceAnnotation
persistent identity: annotation2404610
display id: annotation2404610
version: 1
top level: pSB1A30
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1362091/annotation2404610/range2404610/1

https://synbiohub.org/public/igem/BBa_K1362091/annotation2404610/range2404610/1
type: Range
persistent identity: range2404610
display id: range2404610
version: 1
direction: direction/forward
top level: pSB1A30
start: 2107
end: 2126
orientation: inline

lacO reg
type: SequenceAnnotation
persistent identity: annotation2404631
display id: annotation2404631
version: 1
top level: pSB1A30
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1362091/annotation2404631/range2404631/1

https://synbiohub.org/public/igem/BBa_K1362091/annotation2404631/range2404631/1
type: Range
persistent identity: range2404631
display id: range2404631
version: 1
direction: direction/forward
top level: pSB1A30
start: 2157
end: 2184
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation2404608
display id: annotation2404608
version: 1
top level: pSB1A30
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K1362091/annotation2404608/range2404608/1

https://synbiohub.org/public/igem/BBa_K1362091/annotation2404608/range2404608/1
type: Range
persistent identity: range2404608
display id: range2404608
version: 1
direction: direction/forward
top level: pSB1A30
start: 2018
end: 2037
orientation: inline

ampR (rev)
type: SequenceAnnotation
persistent identity: annotation2404607
display id: annotation2404607
version: 1
top level: pSB1A30
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1362091/annotation2404607/range2404607/1

https://synbiohub.org/public/igem/BBa_K1362091/annotation2404607/range2404607/1
type: Range
persistent identity: range2404607
display id: range2404607
version: 1
direction: direction/reverse
top level: pSB1A30
start: 1035
end: 1895
orientation: inline

E. coli his operon terminator
type: SequenceAnnotation
persistent identity: annotation2404601
display id: annotation2404601
version: 1
top level: pSB1A30
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1362091/annotation2404601/range2404601/1

https://synbiohub.org/public/igem/BBa_K1362091/annotation2404601/range2404601/1
type: Range
persistent identity: range2404601
display id: range2404601
version: 1
direction: direction/forward
top level: pSB1A30
start: 22
end: 93
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation2404632
display id: annotation2404632
version: 1
top level: pSB1A30
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1362091/annotation2404632/range2404632/1

https://synbiohub.org/public/igem/BBa_K1362091/annotation2404632/range2404632/1
type: Range
persistent identity: range2404632
display id: range2404632
version: 1
direction: direction/forward
top level: pSB1A30
start: 2185
end: 2206
orientation: inline

end of terminator
type: SequenceAnnotation
persistent identity: annotation2404609
display id: annotation2404609
version: 1
top level: pSB1A30
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1362091/annotation2404609/range2404609/1

https://synbiohub.org/public/igem/BBa_K1362091/annotation2404609/range2404609/1
type: Range
persistent identity: range2404609
display id: range2404609
version: 1
direction: direction/reverse
top level: pSB1A30
start: 2094
end: 2097
orientation: inline

terminator stem loop
type: SequenceAnnotation
persistent identity: annotation2404603
display id: annotation2404603
version: 1
top level: pSB1A30
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1362091/annotation2404603/range2404603/1

https://synbiohub.org/public/igem/BBa_K1362091/annotation2404603/range2404603/1
type: Range
persistent identity: range2404603
display id: range2404603
version: 1
direction: direction/forward
top level: pSB1A30
start: 30
end: 64
orientation: inline

ORI
type: SequenceAnnotation
persistent identity: annotation2404606
display id: annotation2404606
version: 1
top level: pSB1A30
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1362091/annotation2404606/range2404606/1

https://synbiohub.org/public/igem/BBa_K1362091/annotation2404606/range2404606/1
type: Range
persistent identity: range2404606
display id: range2404606
version: 1
direction: direction/forward
top level: pSB1A30
start: 276
end: 276
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation2404602
display id: annotation2404602
version: 1
top level: pSB1A30
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K1362091/annotation2404602/range2404602/1

https://synbiohub.org/public/igem/BBa_K1362091/annotation2404602/range2404602/1
type: Range
persistent identity: range2404602
display id: range2404602
version: 1
direction: direction/reverse
top level: pSB1A30
start: 157
end: 176
orientation: inline

suffix
type: SequenceAnnotation
persistent identity: annotation2404600
display id: annotation2404600
version: 1
top level: pSB1A30
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1362091/annotation2404600/range2404600/1

https://synbiohub.org/public/igem/BBa_K1362091/annotation2404600/range2404600/1
type: Range
persistent identity: range2404600
display id: range2404600
version: 1
direction: direction/forward
top level: pSB1A30
start: 1
end: 21
orientation: inline

T7 promotor
type: SequenceAnnotation
persistent identity: annotation2404611
display id: annotation2404611
version: 1
top level: pSB1A30
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1362091/annotation2404611/range2404611/1

https://synbiohub.org/public/igem/BBa_K1362091/annotation2404611/range2404611/1
type: Range
persistent identity: range2404611
display id: range2404611
version: 1
direction: direction/forward
top level: pSB1A30
start: 2139
end: 2157
orientation: inline

rep(pMB1) (rev)
type: SequenceAnnotation
persistent identity: annotation2404605
display id: annotation2404605
version: 1
top level: pSB1A30
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1362091/annotation2404605/range2404605/1

https://synbiohub.org/public/igem/BBa_K1362091/annotation2404605/range2404605/1
type: Range
persistent identity: range2404605
display id: range2404605
version: 1
direction: direction/reverse
top level: pSB1A30
start: 261
end: 875
orientation: inline

U073O11+D0
type: ComponentDefinition
persistent identity: BBa I732429
display id: BBa_I732429
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U073O11+D050O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732429_sequence/1

U085O11+D0
type: ComponentDefinition
persistent identity: BBa I732402
display id: BBa_I732402
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U085O11+D062NUL)
creator: Zhan Jian
created: 2007-09-20T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1572
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Might be later edited.
mutable notes: Might be later edited.
mutable provenance: Might be later edited.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: consensus elements
sequence annotation: O1
sequence annotation: +1
sequence: https://synbiohub.org/public/igem/BBa_I732402_sequence/1

consensus elements
type: SequenceAnnotation
persistent identity: annotation1944539
display id: annotation1944539
version: 1
top level: U085O11+D0
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I732402/annotation1944539/range1944539/1

https://synbiohub.org/public/igem/BBa_I732402/annotation1944539/range1944539/1
type: Range
persistent identity: range1944539
display id: range1944539
version: 1
direction: direction/forward
top level: U085O11+D0
start: 69
end: 105
orientation: inline

O1
type: SequenceAnnotation
persistent identity: annotation1944540
display id: annotation1944540
version: 1
top level: U085O11+D0
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I732402/annotation1944540/range1944540/1

https://synbiohub.org/public/igem/BBa_I732402/annotation1944540/range1944540/1
type: Range
persistent identity: range1944540
display id: range1944540
version: 1
direction: direction/forward
top level: U085O11+D0
start: 1
end: 20
orientation: inline

+1
type: SequenceAnnotation
persistent identity: annotation1944541
display id: annotation1944541
version: 1
top level: U085O11+D0
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I732402/annotation1944541/range1944541/1

https://synbiohub.org/public/igem/BBa_I732402/annotation1944541/range1944541/1
type: Range
persistent identity: range1944541
display id: range1944541
version: 1
direction: direction/forward
top level: U085O11+D0
start: 105
end: 105
orientation: inline

BBa_K091100
type: ComponentDefinition
persistent identity: BBa K091100
display id: BBa_K091100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLac_lux hybrid promoter
creator: Pallavi Penumetcha
created: 2008-05-28T11:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _191_
m user id: 0
owner id: 2993
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This promoter is repressed by lacI and luxR with HSL. It contains a lux box and a lacI binding site.
mutable notes: Deleted the CAP binding site in R0010.
mutable provenance: R0010 modified with the insertion of a lux box in between -35 and -10.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: lux box
sequence annotation: -10
sequence annotation: -35
sequence annotation: lacI binding site
sequence: https://synbiohub.org/public/igem/BBa_K091100_sequence/1

lux box
type: SequenceAnnotation
persistent identity: annotation1963335
display id: annotation1963335
version: 1
top level: BBa_K091100
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K091100/annotation1963335/range1963335/1

https://synbiohub.org/public/igem/BBa_K091100/annotation1963335/range1963335/1
type: Range
persistent identity: range1963335
display id: range1963335
version: 1
direction: direction/forward
top level: BBa_K091100
start: 16
end: 33
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1963334
display id: annotation1963334
version: 1
top level: BBa_K091100
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K091100/annotation1963334/range1963334/1

https://synbiohub.org/public/igem/BBa_K091100/annotation1963334/range1963334/1
type: Range
persistent identity: range1963334
display id: range1963334
version: 1
direction: direction/forward
top level: BBa_K091100
start: 34
end: 39
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1963333
display id: annotation1963333
version: 1
top level: BBa_K091100
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K091100/annotation1963333/range1963333/1

https://synbiohub.org/public/igem/BBa_K091100/annotation1963333/range1963333/1
type: Range
persistent identity: range1963333
display id: range1963333
version: 1
direction: direction/forward
top level: BBa_K091100
start: 10
end: 15
orientation: inline

lacI binding site
type: SequenceAnnotation
persistent identity: annotation1963336
display id: annotation1963336
version: 1
top level: BBa_K091100
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K091100/annotation1963336/range1963336/1

https://synbiohub.org/public/igem/BBa_K091100/annotation1963336/range1963336/1
type: Range
persistent identity: range1963336
display id: range1963336
version: 1
direction: direction/forward
top level: BBa_K091100
start: 40
end: 74
orientation: inline

BBa_K266005
type: ComponentDefinition
persistent identity: BBa K266005
display id: BBa_K266005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PAI+LasR -> LasI & AI+LuxR --| LasI
creator: UNAM FCIENCIAS-CINVESTAV IRA
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _363_
m user id: 0
owner id: 1955
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: BBa_K091146 promoter (PAI+LasR inducible & AI+LuxR repressible) controls the expresion of LasI enzyme, no terminator
mutable notes: None
mutable provenance: The biobricks BBa_K091146 and BBa_S03154 where used to build this biobrick
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_S03154
component: BBa_K091146
sequence annotation: BBa_S03154
sequence annotation: BBa_K091146
sequence: https://synbiohub.org/public/igem/BBa_K266005_sequence/1

BBa_S03154
type: Component
persistent identity: component2246684
display id: component2246684
version: 1
top level: BBa_K266005
access: public
definition: https://synbiohub.org/public/igem/BBa_S03154/1

BBa_K091146
type: Component
persistent identity: component2246677
display id: component2246677
version: 1
top level: BBa_K266005
access: public
definition: BBa_K091146

BBa_S03154
type: SequenceAnnotation
persistent identity: annotation2246684
display id: annotation2246684
version: 1
top level: BBa_K266005
location: https://synbiohub.org/public/igem/BBa_K266005/annotation2246684/range2246684/1
component: BBa_S03154

https://synbiohub.org/public/igem/BBa_K266005/annotation2246684/range2246684/1
type: Range
persistent identity: range2246684
display id: range2246684
version: 1
direction: direction/reverse
top level: BBa_K266005
start: 135
end: 819
orientation: inline

BBa_K091146
type: SequenceAnnotation
persistent identity: annotation2246677
display id: annotation2246677
version: 1
top level: BBa_K266005
location: https://synbiohub.org/public/igem/BBa_K266005/annotation2246677/range2246677/1
component: BBa_K091146

https://synbiohub.org/public/igem/BBa_K266005/annotation2246677/range2246677/1
type: Range
persistent identity: range2246677
display id: range2246677
version: 1
direction: direction/reverse
top level: BBa_K266005
start: 1
end: 126
orientation: inline

BBa_J05218
type: ComponentDefinition
persistent identity: BBa J05218
display id: BBa_J05218
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Regulator for R4-cMaf
creator: Alexander Roth
created: 2005-09-07T11:00:00Z
modified: 2015-08-31T04:08:15Z
discontinued: false
dominant: false
group u list: _34_
m user id: 0
owner id: 412
owning group id: 34
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: binding sites for R1-CREBH and R2-YAP7
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J05218_sequence/1

BBa_K121011
type: ComponentDefinition
persistent identity: BBa K121011
display id: BBa_K121011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: promoter (lacI regulated)
creator: Masashi Kajita
created: 2008-10-15T11:00:00Z
modified: 2015-05-08T01:09:41Z
discontinued: false
dominant: false
group u list: _220_
m user id: 0
owner id: 3180
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: pressure inducible lac promoter
mutable notes: na
mutable provenance: na
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: -35
sequence annotation: K121011
sequence annotation: CAP binding site
sequence annotation: LacI binding site
sequence: https://synbiohub.org/public/igem/BBa_K121011_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation1981861
display id: annotation1981861
version: 1
top level: BBa_K121011
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K121011/annotation1981861/range1981861/1

https://synbiohub.org/public/igem/BBa_K121011/annotation1981861/range1981861/1
type: Range
persistent identity: range1981861
display id: range1981861
version: 1
direction: direction/forward
top level: BBa_K121011
start: 165
end: 168
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1981860
display id: annotation1981860
version: 1
top level: BBa_K121011
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K121011/annotation1981860/range1981860/1

https://synbiohub.org/public/igem/BBa_K121011/annotation1981860/range1981860/1
type: Range
persistent identity: range1981860
display id: range1981860
version: 1
direction: direction/forward
top level: BBa_K121011
start: 141
end: 146
orientation: inline

K121011
type: SequenceAnnotation
persistent identity: annotation1981858
display id: annotation1981858
version: 1
top level: BBa_K121011
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K121011/annotation1981858/range1981858/1

https://synbiohub.org/public/igem/BBa_K121011/annotation1981858/range1981858/1
type: Range
persistent identity: range1981858
display id: range1981858
version: 1
direction: direction/forward
top level: BBa_K121011
start: 1
end: 232
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1981859
display id: annotation1981859
version: 1
top level: BBa_K121011
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K121011/annotation1981859/range1981859/1

https://synbiohub.org/public/igem/BBa_K121011/annotation1981859/range1981859/1
type: Range
persistent identity: range1981859
display id: range1981859
version: 1
direction: direction/forward
top level: BBa_K121011
start: 94
end: 131
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1981862
display id: annotation1981862
version: 1
top level: BBa_K121011
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K121011/annotation1981862/range1981862/1

https://synbiohub.org/public/igem/BBa_K121011/annotation1981862/range1981862/1
type: Range
persistent identity: range1981862
display id: range1981862
version: 1
direction: direction/forward
top level: BBa_K121011
start: 169
end: 203
orientation: inline

NAND2616
type: ComponentDefinition
persistent identity: BBa I732301
display id: BBa_I732301
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NAND Candidate (U073O26D001O16)
creator: Ma Rui
created: 2007-10-02T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1592
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: U97NUL D001O11
mutable notes: N/A
mutable provenance: LacIq
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732301_sequence/1

NANDbeta
type: ComponentDefinition
persistent identity: BBa I732306
display id: BBa_I732306
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NAND Candidate (U073O11D002O22)
creator: Zhan Jian
created: 2007-10-25T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: {| border="1" | |align="center"| |align="center"| |}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732306_sequence/1

U097NUL+D0
type: ComponentDefinition
persistent identity: BBa I732410
display id: BBa_I732410
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U097NUL+D038O22)
creator: Zhan Jian
created: 2007-09-22T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1572
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To be later edited if time permits.
mutable notes: To be later edited if time permits.
mutable provenance: To be later edited if time permits.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: O2
sequence annotation: consensus elements
sequence annotation: +1
sequence: https://synbiohub.org/public/igem/BBa_I732410_sequence/1

O2
type: SequenceAnnotation
persistent identity: annotation1944559
display id: annotation1944559
version: 1
top level: U097NUL+D0
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I732410/annotation1944559/range1944559/1

https://synbiohub.org/public/igem/BBa_I732410/annotation1944559/range1944559/1
type: Range
persistent identity: range1944559
display id: range1944559
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 134
end: 153
orientation: inline

consensus elements
type: SequenceAnnotation
persistent identity: annotation1944558
display id: annotation1944558
version: 1
top level: U097NUL+D0
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I732410/annotation1944558/range1944558/1

https://synbiohub.org/public/igem/BBa_I732410/annotation1944558/range1944558/1
type: Range
persistent identity: range1944558
display id: range1944558
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 67
end: 137
orientation: inline

+1
type: SequenceAnnotation
persistent identity: annotation1944560
display id: annotation1944560
version: 1
top level: U097NUL+D0
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I732410/annotation1944560/range1944560/1

https://synbiohub.org/public/igem/BBa_I732410/annotation1944560/range1944560/1
type: Range
persistent identity: range1944560
display id: range1944560
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 137
end: 137
orientation: inline

BBa_K566006
type: ComponentDefinition
persistent identity: BBa K566006
display id: BBa_K566006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: penI-pCpcG2-pPenI-mnt
creator: Daniel Rodriguez and Porfirio Quintero
created: 2011-09-25T11:00:00Z
modified: 2015-05-08T01:12:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _734_
m user id: 0
owner id: 8650
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: Potential and gate. Composed of pCpcG2 promoter which may be inducible by green light, this way it express penI and this represses mnt expression, allowing the transcription of any gene controlled under pMnt promoter. This construction may be used with another one which represses mnt expression with red light and this way it may act as an and-gate.
mutable notes: Search for illegal sites was performed, they were found and removed.
mutable provenance: Sequence of pCpcG2 promoter was obtained from pJT122 plasmid of Dr. Jeff Tabor. Rest of the sequences were obtained from the Registry. Complete construction was synthesized.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K566010
component: BBa_K566009
component: BBa_R0074
component: BBa_B0034
component: BBa_K566008
sequence annotation: BBa_B0034
sequence annotation: BBa_K566010
sequence annotation: BBa_K566009
sequence annotation: BBa_R0074
sequence annotation: BBa_K566008
sequence: https://synbiohub.org/public/igem/BBa_K566006_sequence/1

BBa_K566010
type: Component
persistent identity: component2142632
display id: component2142632
version: 1
top level: BBa_K566006
access: public
definition: https://synbiohub.org/public/igem/BBa_K566010/1

BBa_K566009
type: Component
persistent identity: component2142636
display id: component2142636
version: 1
top level: BBa_K566006
access: public
definition: https://synbiohub.org/public/igem/BBa_K566009/1

BBa_R0074
type: Component
persistent identity: component2142642
display id: component2142642
version: 1
top level: BBa_K566006
access: public
definition: https://synbiohub.org/public/igem/BBa_R0074/1

BBa_B0034
type: Component
persistent identity: component2142644
display id: component2142644
version: 1
top level: BBa_K566006
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K566008
type: Component
persistent identity: component2142647
display id: component2142647
version: 1
top level: BBa_K566006
access: public
definition: https://synbiohub.org/public/igem/BBa_K566008/1

BBa_B0034
type: Component
persistent identity: component2142634
display id: component2142634
version: 1
top level: BBa_K566006
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2142634
display id: annotation2142634
version: 1
top level: BBa_K566006
location: https://synbiohub.org/public/igem/BBa_K566006/annotation2142634/range2142634/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K566006/annotation2142634/range2142634/1
type: Range
persistent identity: range2142634
display id: range2142634
version: 1
direction: direction/reverse
top level: BBa_K566006
start: 424
end: 435
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2142644
display id: annotation2142644
version: 1
top level: BBa_K566006
location: https://synbiohub.org/public/igem/BBa_K566006/annotation2142644/range2142644/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K566006/annotation2142644/range2142644/1
type: Range
persistent identity: range2142644
display id: range2142644
version: 1
direction: direction/reverse
top level: BBa_K566006
start: 753
end: 764
orientation: inline

BBa_K566010
type: SequenceAnnotation
persistent identity: annotation2142632
display id: annotation2142632
version: 1
top level: BBa_K566006
location: https://synbiohub.org/public/igem/BBa_K566006/annotation2142632/range2142632/1
component: BBa_K566010

https://synbiohub.org/public/igem/BBa_K566006/annotation2142632/range2142632/1
type: Range
persistent identity: range2142632
display id: range2142632
version: 1
direction: direction/reverse
top level: BBa_K566006
start: 1
end: 423
orientation: inline

BBa_K566009
type: SequenceAnnotation
persistent identity: annotation2142636
display id: annotation2142636
version: 1
top level: BBa_K566006
location: https://synbiohub.org/public/igem/BBa_K566006/annotation2142636/range2142636/1
component: BBa_K566009

https://synbiohub.org/public/igem/BBa_K566006/annotation2142636/range2142636/1
type: Range
persistent identity: range2142636
display id: range2142636
version: 1
direction: direction/reverse
top level: BBa_K566006
start: 436
end: 675
orientation: inline

BBa_R0074
type: SequenceAnnotation
persistent identity: annotation2142642
display id: annotation2142642
version: 1
top level: BBa_K566006
location: https://synbiohub.org/public/igem/BBa_K566006/annotation2142642/range2142642/1
component: BBa_R0074

https://synbiohub.org/public/igem/BBa_K566006/annotation2142642/range2142642/1
type: Range
persistent identity: range2142642
display id: range2142642
version: 1
direction: direction/reverse
top level: BBa_K566006
start: 676
end: 752
orientation: inline

BBa_K566008
type: SequenceAnnotation
persistent identity: annotation2142647
display id: annotation2142647
version: 1
top level: BBa_K566006
location: https://synbiohub.org/public/igem/BBa_K566006/annotation2142647/range2142647/1
component: BBa_K566008

https://synbiohub.org/public/igem/BBa_K566006/annotation2142647/range2142647/1
type: Range
persistent identity: range2142647
display id: range2142647
version: 1
direction: direction/reverse
top level: BBa_K566006
start: 765
end: 1052
orientation: inline

BBa_K112402
type: ComponentDefinition
persistent identity: BBa K112402
display id: BBa_K112402
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: promoter for FabA gene - Membrane Damage and Ultrasound Senstitive
creator: Christina Brown
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:17Z
discontinued: false
dominant: false
group u list: _224_
m user id: 0
owner id: 3241
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: According to Vollmer, et. al in their 1998 Applied and Enviormental Microbiology paper, "Bacterial Stress Responses to 1-Megahertz Pulsed Ultrasound in the Presence of Microbubble", the promoter for the fabA gene, a known membrane damage response gene, is upregulated seven fold when cells are exposed to high frequency ultrasound and microbubble cavitation.
mutable notes: none
mutable provenance: cloned from E. coli MG1655 genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K112402_sequence/1

U097O11+D0
type: ComponentDefinition
persistent identity: BBa I732417
display id: BBa_I732417
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U097O11+D050O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732417_sequence/1

U073O11+D0
type: ComponentDefinition
persistent identity: BBa I732403
display id: BBa_I732403
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U073O11+D062NUL)
creator: Zhan Jian
created: 2007-09-20T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1572
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Might be later edited.
mutable notes: Might be later edited.
mutable provenance: Might be later edited.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: consensus elements
sequence annotation: +1
sequence annotation: O1
sequence: https://synbiohub.org/public/igem/BBa_I732403_sequence/1

consensus elements
type: SequenceAnnotation
persistent identity: annotation1944542
display id: annotation1944542
version: 1
top level: U073O11+D0
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I732403/annotation1944542/range1944542/1

https://synbiohub.org/public/igem/BBa_I732403/annotation1944542/range1944542/1
type: Range
persistent identity: range1944542
display id: range1944542
version: 1
direction: direction/forward
top level: U073O11+D0
start: 57
end: 93
orientation: inline

+1
type: SequenceAnnotation
persistent identity: annotation1944544
display id: annotation1944544
version: 1
top level: U073O11+D0
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I732403/annotation1944544/range1944544/1

https://synbiohub.org/public/igem/BBa_I732403/annotation1944544/range1944544/1
type: Range
persistent identity: range1944544
display id: range1944544
version: 1
direction: direction/forward
top level: U073O11+D0
start: 93
end: 93
orientation: inline

O1
type: SequenceAnnotation
persistent identity: annotation1944543
display id: annotation1944543
version: 1
top level: U073O11+D0
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I732403/annotation1944543/range1944543/1

https://synbiohub.org/public/igem/BBa_I732403/annotation1944543/range1944543/1
type: Range
persistent identity: range1944543
display id: range1944543
version: 1
direction: direction/forward
top level: U073O11+D0
start: 1
end: 20
orientation: inline

BBa_K1486013
type: ComponentDefinition
persistent identity: BBa K1486013
display id: BBa_K1486013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CpxR-IFP[2] under pBAD
creator: iGem EPFL 2014
created: 2014-08-31T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part has a fused protein downstream of an arabinose-inducible promoter: CpxR-IFP[2]. Biobrick BBa_K1486012 has CpxR-IFP[1] with an arabinose promoter. This construct was made in both orientations: BBa_K1486015 is IFP[2]-CpxR (with an arabinose promoter).
mutable notes: Both orientations were made, as well as for IFP[1] so all dimerizations could be tested (see main page for biobrick numbers). Flexible linkers were used to fuse both proteins, and an RBS was put before each one of them.
mutable provenance: to edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1486007
component: BBa_I0500
component: BBa_K1486000
component: BBa_K1486004
component: BBa_B0034
sequence annotation: BBa_K1486000
sequence annotation: BBa_B0034
sequence annotation: BBa_I0500
sequence annotation: BBa_K1486007
sequence annotation: BBa_K1486004
sequence: https://synbiohub.org/public/igem/BBa_K1486013_sequence/1

BBa_K1486007
type: Component
persistent identity: component2382308
display id: component2382308
version: 1
top level: BBa_K1486013
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486007/1

BBa_I0500
type: Component
persistent identity: component2382297
display id: component2382297
version: 1
top level: BBa_K1486013
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_K1486000
type: Component
persistent identity: component2382303
display id: component2382303
version: 1
top level: BBa_K1486013
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_K1486004
type: Component
persistent identity: component2382304
display id: component2382304
version: 1
top level: BBa_K1486013
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_B0034
type: Component
persistent identity: component2382299
display id: component2382299
version: 1
top level: BBa_K1486013
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2382303
display id: annotation2382303
version: 1
top level: BBa_K1486013
location: https://synbiohub.org/public/igem/BBa_K1486013/annotation2382303/range2382303/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486013/annotation2382303/range2382303/1
type: Range
persistent identity: range2382303
display id: range2382303
version: 1
direction: direction/reverse
top level: BBa_K1486013
start: 1223
end: 1924
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2382299
display id: annotation2382299
version: 1
top level: BBa_K1486013
location: https://synbiohub.org/public/igem/BBa_K1486013/annotation2382299/range2382299/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486013/annotation2382299/range2382299/1
type: Range
persistent identity: range2382299
display id: range2382299
version: 1
direction: direction/reverse
top level: BBa_K1486013
start: 1211
end: 1222
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2382297
display id: annotation2382297
version: 1
top level: BBa_K1486013
location: https://synbiohub.org/public/igem/BBa_K1486013/annotation2382297/range2382297/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486013/annotation2382297/range2382297/1
type: Range
persistent identity: range2382297
display id: range2382297
version: 1
direction: direction/reverse
top level: BBa_K1486013
start: 1
end: 1210
orientation: inline

BBa_K1486007
type: SequenceAnnotation
persistent identity: annotation2382308
display id: annotation2382308
version: 1
top level: BBa_K1486013
location: https://synbiohub.org/public/igem/BBa_K1486013/annotation2382308/range2382308/1
component: BBa_K1486007

https://synbiohub.org/public/igem/BBa_K1486013/annotation2382308/range2382308/1
type: Range
persistent identity: range2382308
display id: range2382308
version: 1
direction: direction/reverse
top level: BBa_K1486013
start: 1955
end: 2533
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2382304
display id: annotation2382304
version: 1
top level: BBa_K1486013
location: https://synbiohub.org/public/igem/BBa_K1486013/annotation2382304/range2382304/1
component: BBa_K1486004

https://synbiohub.org/public/igem/BBa_K1486013/annotation2382304/range2382304/1
type: Range
persistent identity: range2382304
display id: range2382304
version: 1
direction: direction/reverse
top level: BBa_K1486013
start: 1925
end: 1954
orientation: inline

pUSPA
type: ComponentDefinition
persistent identity: BBa K1620000
display id: BBa_K1620000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter element UspA
creator: Celio Dias Santos Jr
created: 2015-09-02T11:00:00Z
modified: 2015-09-05T12:59:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2037_
m user id: 23597
owner id: 23597
owning group id: 9
status: status/Planning
bookmark: true
mutable description: The universal stress protein A is a response of Escherichia coli cells to growth arrest, and its lacking generates cells with defective growth.This genetic element is dependent to sigma-70 factor. Its regulation is done through the concentration of a specific stationary phase allormone, guanosine-5'-diphosphate-3'-diphosphate (ppGp), as described elsewhere. The ppGp activate the transcription of downstream elements through a positive regulation of the β-subunit of RNA polymerase. In this sense, the PuspA element is considered a stationary phase promoter. However, its behavior is like a constitutive transcription promotion, becoming higher in stress situations. Diverse conditions make the Escherichia coli cell enter to stress state, like heat shocks, starvation, osmotic stress, ultraviolet light and other conditions. In these situations, the RNA polimerase sigma factors (ropS) trigger the expression of chaperones and other stress protector molecules, in order to help the cell survive. In our project we have used this promoter to trigger the expression of limonene synthase after an osmotic shock. Other uses of this part includes the environmental stress detectors making using a reporter system coupled to this promoter.
mutable notes: The original promoter site was shortened and the last three nucleotides were changed to restriction site adjustment.
mutable provenance: We have drawn the sequence for an improved PuspA promoter from an analysis of 400bp upstream the Universal Protein A (UspA) gene in E. coli K12 MG16555 genome. After design, the DNA template of part was synthesized by IDT and was amplified from a G-block and assembled using 3A assembly method.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: UspA
sequence: https://synbiohub.org/public/igem/BBa_K1620000_sequence/1

UspA
type: SequenceAnnotation
persistent identity: annotation2443284
display id: annotation2443284
version: 1
top level: pUSPA
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1620000/annotation2443284/range2443284/1

https://synbiohub.org/public/igem/BBa_K1620000/annotation2443284/range2443284/1
type: Range
persistent identity: range2443284
display id: range2443284
version: 1
direction: direction/forward
top level: pUSPA
start: 1
end: 46
orientation: inline

BBa_K177038
type: ComponentDefinition
persistent identity: BBa K177038
display id: BBa_K177038
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Bistable switch 1 testing device
creator: Anna Olchowik
created: 2009-09-08T11:00:00Z
modified: 2015-05-08T01:11:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _284_
m user id: 0
owner id: 4959
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K094130
component: BBa_K177012
component: BBa_K177034
sequence annotation: BBa_K094130
sequence annotation: BBa_K177012
sequence annotation: BBa_K177034
sequence: https://synbiohub.org/public/igem/BBa_K177038_sequence/1

BBa_K094130
type: Component
persistent identity: component2302528
display id: component2302528
version: 1
top level: BBa_K177038
access: public
definition: https://synbiohub.org/public/igem/BBa_K094130/1

BBa_K177012
type: Component
persistent identity: component2302516
display id: component2302516
version: 1
top level: BBa_K177038
access: public
definition: https://synbiohub.org/public/igem/BBa_K177012/1

BBa_K177034
type: Component
persistent identity: component2302503
display id: component2302503
version: 1
top level: BBa_K177038
access: public
definition: https://synbiohub.org/public/igem/BBa_K177034/1

BBa_K094130
type: SequenceAnnotation
persistent identity: annotation2302528
display id: annotation2302528
version: 1
top level: BBa_K177038
location: https://synbiohub.org/public/igem/BBa_K177038/annotation2302528/range2302528/1
component: BBa_K094130

https://synbiohub.org/public/igem/BBa_K177038/annotation2302528/range2302528/1
type: Range
persistent identity: range2302528
display id: range2302528
version: 1
direction: direction/reverse
top level: BBa_K177038
start: 3073
end: 3933
orientation: inline

BBa_K177012
type: SequenceAnnotation
persistent identity: annotation2302516
display id: annotation2302516
version: 1
top level: BBa_K177038
location: https://synbiohub.org/public/igem/BBa_K177038/annotation2302516/range2302516/1
component: BBa_K177012

https://synbiohub.org/public/igem/BBa_K177038/annotation2302516/range2302516/1
type: Range
persistent identity: range2302516
display id: range2302516
version: 1
direction: direction/reverse
top level: BBa_K177038
start: 1836
end: 3064
orientation: inline

BBa_K177034
type: SequenceAnnotation
persistent identity: annotation2302503
display id: annotation2302503
version: 1
top level: BBa_K177038
location: https://synbiohub.org/public/igem/BBa_K177038/annotation2302503/range2302503/1
component: BBa_K177034

https://synbiohub.org/public/igem/BBa_K177038/annotation2302503/range2302503/1
type: Range
persistent identity: range2302503
display id: range2302503
version: 1
direction: direction/reverse
top level: BBa_K177038
start: 1
end: 1827
orientation: inline

U085O11+D0
type: ComponentDefinition
persistent identity: BBa I732422
display id: BBa_I732422
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U085O11+D038O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732422_sequence/1

FlipFlop
type: ComponentDefinition
persistent identity: BBa K907002
display id: BBa_K907002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Binary signal generator, RBS(reverse) - attB - Promoter - attP - RBS
creator: Dong-hui Choe, Soo-in Lee
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1172_
m user id: 0
owner id: 12628
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Dual phase protein generator promoter reversed, RBS(reverse) - attB - Promoter(reverse) - attP - RBS This part is composed of 3 elements. 1. RBS: BBa_B0034, upsteam one is reversed 2. Promoter: BBa_J23119, Bacterial constitutive promoter 3. att site: Recognition site for Mycobacteriophage Bxb1 integrase/excisionase We designed this part to generate two different proteins one at a time. At the first state, this device promotes transcription and translation of downstream gene because its promoter orientation. When Mycobacteriophage Bxb1 integrase recognizes and inverts sequence flanking with attB and attP sequence, promoter orientation reversed. Then this device promotes transcription and translation of upstream gene(must be reversed at the construction step). This device is tested by GFP and mRFP attached construct. See BBa_K090700N and BBa_K090700N
mutable notes: No
mutable provenance: Promoter and RBS are derived from BBa_J23119 and BBa_B0034, respectively. Whole construct was de novo synthesized by Bioneer.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: attB(Bxb1 Int)
sequence annotation: BBa_J23119
sequence annotation: BBa_B0034
sequence annotation: attP(Bxb1 Int)
sequence annotation: BBa_B0034, reverse
sequence: https://synbiohub.org/public/igem/BBa_K907002_sequence/1

attB(Bxb1 Int)
type: SequenceAnnotation
persistent identity: annotation2189782
display id: annotation2189782
version: 1
top level: FlipFlop
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K907002/annotation2189782/range2189782/1

https://synbiohub.org/public/igem/BBa_K907002/annotation2189782/range2189782/1
type: Range
persistent identity: range2189782
display id: range2189782
version: 1
direction: direction/forward
top level: FlipFlop
start: 19
end: 68
orientation: inline

BBa_J23119
type: SequenceAnnotation
persistent identity: annotation2189785
display id: annotation2189785
version: 1
top level: FlipFlop
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K907002/annotation2189785/range2189785/1

https://synbiohub.org/public/igem/BBa_K907002/annotation2189785/range2189785/1
type: Range
persistent identity: range2189785
display id: range2189785
version: 1
direction: direction/forward
top level: FlipFlop
start: 69
end: 103
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2188633
display id: annotation2188633
version: 1
top level: FlipFlop
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K907002/annotation2188633/range2188633/1

https://synbiohub.org/public/igem/BBa_K907002/annotation2188633/range2188633/1
type: Range
persistent identity: range2188633
display id: range2188633
version: 1
direction: direction/forward
top level: FlipFlop
start: 163
end: 174
orientation: inline

attP(Bxb1 Int)
type: SequenceAnnotation
persistent identity: annotation2189784
display id: annotation2189784
version: 1
top level: FlipFlop
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K907002/annotation2189784/range2189784/1

https://synbiohub.org/public/igem/BBa_K907002/annotation2189784/range2189784/1
type: Range
persistent identity: range2189784
display id: range2189784
version: 1
direction: direction/forward
top level: FlipFlop
start: 104
end: 156
orientation: inline

BBa_B0034, reverse
type: SequenceAnnotation
persistent identity: annotation2188632
display id: annotation2188632
version: 1
top level: FlipFlop
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K907002/annotation2188632/range2188632/1

https://synbiohub.org/public/igem/BBa_K907002/annotation2188632/range2188632/1
type: Range
persistent identity: range2188632
display id: range2188632
version: 1
direction: direction/reverse
top level: FlipFlop
start: 1
end: 12
orientation: inline

BBa_K116001
type: ComponentDefinition
persistent identity: BBa K116001
display id: BBa_K116001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: nhaA promoter, that can be regulated by pH and nhaR protein.
creator: Chih-Hsien Yang
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:09:32Z
discontinued: false
dominant: true
group u list: _210_
m user id: 0
owner id: 592
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: It's E.coli K12 sodium/proton anti-transport promoter. It can be a pH sensor. We test pH senses in different pH value and Sodium concentration.
mutable notes: It should contain NhaR binding site.
mutable provenance: E.coli K12 MG1655 genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K116001_sequence/1

BBa_J64800
type: ComponentDefinition
persistent identity: BBa J64800
display id: BBa_J64800
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RHLR/RHLI Inducible & LasR/LasI repressible Promoter
creator: laura lavery
created: 2007-03-24T12:00:00Z
modified: 2015-05-08T01:08:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 1431
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is similar to BBa_R0079 but has an added binding site for RHLR/RHLI in between the -35 and -10 region. Thus this promoter is induced by LasR/LasI but repressed by RHLR/RHLi binding.
mutable notes: There is a 17bp spacer between the -35 and -10 regions and the binding site for RHLR/RHLI is 20bp long. I was able to fit the whole binding site in the spacer region because the last three base pairs of the -35 region and the first three base pairs of the RHLR/RHLI binding site are the same.
mutable provenance: Rust L. et. al., "Analysis of the Pseudomonas aeruginosa Elastase (lasB) Regulatory Region". Journal of Bacteriology, 1996.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: LasR/LasI
sequence annotation: -10
sequence annotation: RHLR/RHLI
sequence: https://synbiohub.org/public/igem/BBa_J64800_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation1921062
display id: annotation1921062
version: 1
top level: BBa_J64800
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J64800/annotation1921062/range1921062/1

https://synbiohub.org/public/igem/BBa_J64800/annotation1921062/range1921062/1
type: Range
persistent identity: range1921062
display id: range1921062
version: 1
direction: direction/forward
top level: BBa_J64800
start: 19
end: 24
orientation: inline

LasR/LasI
type: SequenceAnnotation
persistent identity: annotation1921061
display id: annotation1921061
version: 1
top level: BBa_J64800
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_J64800/annotation1921061/range1921061/1

https://synbiohub.org/public/igem/BBa_J64800/annotation1921061/range1921061/1
type: Range
persistent identity: range1921061
display id: range1921061
version: 1
direction: direction/forward
top level: BBa_J64800
start: 23
end: 40
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1921063
display id: annotation1921063
version: 1
top level: BBa_J64800
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J64800/annotation1921063/range1921063/1

https://synbiohub.org/public/igem/BBa_J64800/annotation1921063/range1921063/1
type: Range
persistent identity: range1921063
display id: range1921063
version: 1
direction: direction/forward
top level: BBa_J64800
start: 42
end: 47
orientation: inline

RHLR/RHLI
type: SequenceAnnotation
persistent identity: annotation1921060
display id: annotation1921060
version: 1
top level: BBa_J64800
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J64800/annotation1921060/range1921060/1

https://synbiohub.org/public/igem/BBa_J64800/annotation1921060/range1921060/1
type: Range
persistent identity: range1921060
display id: range1921060
version: 1
direction: direction/forward
top level: BBa_J64800
start: 1
end: 23
orientation: inline

BBa_K259005
type: ComponentDefinition
persistent identity: BBa K259005
display id: BBa_K259005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AraC Rheostat Promoter
creator: Petros Mina
created: 2009-09-02T11:00:00Z
modified: 2015-05-08T01:11:42Z
discontinued: false
dominant: false
group u list: _357_
m user id: 0
owner id: 5318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The AraC/pBAD promoter can be used with prokaryotic chassis to regulate expresion of proteins. When the AraC/pBAD promoter is placed upstream of your canditate translational unit (protein to be expressed) it allows control of the expression of the said protein. This is done via controlling the concentration of arabinose sugar in the environment/solution of bacterial growth. This composite part does not have an LVA degradation tag at its end, hence can be used with all prokaryotic assemblies.
mutable notes: The part was designed and assembled from the iGEM2009 kit with standard RFC10 assembly.
mutable provenance: The AraC promoter is found naturally in E.Coli at 1.52 min/centisome in the clockwise orientation. More information can be found at [http://ecogene.org/geneinfo.php?eg_id=EG10054 EcoGene].
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0081
component: BBa_R0080
sequence annotation: BBa_R0080
sequence annotation: BBa_R0081
sequence: https://synbiohub.org/public/igem/BBa_K259005_sequence/1

BBa_R0081
type: Component
persistent identity: component2018287
display id: component2018287
version: 1
top level: BBa_K259005
access: public
definition: https://synbiohub.org/public/igem/BBa_R0081/1

BBa_R0080
type: Component
persistent identity: component2018294
display id: component2018294
version: 1
top level: BBa_K259005
access: public
definition: https://synbiohub.org/public/igem/BBa_R0080/1

BBa_R0080
type: SequenceAnnotation
persistent identity: annotation2018294
display id: annotation2018294
version: 1
top level: BBa_K259005
location: https://synbiohub.org/public/igem/BBa_K259005/annotation2018294/range2018294/1
component: BBa_R0080

https://synbiohub.org/public/igem/BBa_K259005/annotation2018294/range2018294/1
type: Range
persistent identity: range2018294
display id: range2018294
version: 1
direction: direction/reverse
top level: BBa_K259005
start: 192
end: 340
orientation: inline

BBa_R0081
type: SequenceAnnotation
persistent identity: annotation2018287
display id: annotation2018287
version: 1
top level: BBa_K259005
location: https://synbiohub.org/public/igem/BBa_K259005/annotation2018287/range2018287/1
component: BBa_R0081

https://synbiohub.org/public/igem/BBa_K259005/annotation2018287/range2018287/1
type: Range
persistent identity: range2018287
display id: range2018287
version: 1
direction: direction/reverse
top level: BBa_K259005
start: 1
end: 183
orientation: inline

BBa_I12040
type: ComponentDefinition
persistent identity: BBa I12040
display id: BBa_I12040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Modified lambda P(RM) promoter: -10 region from P(L) and cooperatively repressed by 434 cI
creator: ryhsiao
created: 2004-08-18T11:00:00Z
modified: 2015-08-31T04:07:31Z
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 148
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Lamdba P(RM) promoter modified to be activated by lamda repressor (cI) and repressed by 434 repressor (cI). Two operator sites are used for both activation and repression to enhance cooperativity. Additionally, the -10 region from lambda P(L) was used instead of that from P(RM).
mutable notes: The O-R3 site of lambda (17 nt) was replaced by the O-R1 site of 434 (14 nt) to facilitate repression by 434 cI. The new 434 O-R1 is 5'-justified with the lambda O-R3 it replaced, leaving the -10 sequence intact. The 434 O-R2 site replaces the wildtype labmda promoter starting at -5, thereby preserving the 8 nt between 434 O-R1 and O-R2 in the wildtype. The mRNA transcript will contain an additional 9 nt compared to wildtype lambda phage. This part differs from BBa_I12036 because the -10 region from P(L) was used instead of that from P(RM).
mutable provenance: Bushman, F. D. The bacteriophage 434 right operator roles of O-R1, O-R2, and O-R3. J. Mol. Biol. (1993) 230, 28-40. ; Lutz, R & Bujard, H. "Independent and tight regulation of transcriptional units in Escherichia coli via the lacR/O, the TetR/O and AraC/I1-I2 regulatory elements, Nucleic Acids Research, 1997, Vol.25, No.6 (1203-1210)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OR1 434
sequence annotation: start
sequence annotation: OR2 lambda
sequence annotation: OR2 434
sequence annotation: -35
sequence annotation: OR1 lambda
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_I12040_sequence/1

OR1 434
type: SequenceAnnotation
persistent identity: annotation1059301
display id: annotation1059301
version: 1
top level: BBa_I12040
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12040/annotation1059301/range1059301/1

https://synbiohub.org/public/igem/BBa_I12040/annotation1059301/range1059301/1
type: Range
persistent identity: range1059301
display id: range1059301
version: 1
direction: direction/forward
top level: BBa_I12040
start: 56
end: 69
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1062263
display id: annotation1062263
version: 1
top level: BBa_I12040
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I12040/annotation1062263/range1062263/1

https://synbiohub.org/public/igem/BBa_I12040/annotation1062263/range1062263/1
type: Range
persistent identity: range1062263
display id: range1062263
version: 1
direction: direction/forward
top level: BBa_I12040
start: 83
end: 83
orientation: inline

OR2 lambda
type: SequenceAnnotation
persistent identity: annotation1059299
display id: annotation1059299
version: 1
top level: BBa_I12040
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12040/annotation1059299/range1059299/1

https://synbiohub.org/public/igem/BBa_I12040/annotation1059299/range1059299/1
type: Range
persistent identity: range1059299
display id: range1059299
version: 1
direction: direction/forward
top level: BBa_I12040
start: 33
end: 49
orientation: inline

OR2 434
type: SequenceAnnotation
persistent identity: annotation1059303
display id: annotation1059303
version: 1
top level: BBa_I12040
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12040/annotation1059303/range1059303/1

https://synbiohub.org/public/igem/BBa_I12040/annotation1059303/range1059303/1
type: Range
persistent identity: range1059303
display id: range1059303
version: 1
direction: direction/forward
top level: BBa_I12040
start: 78
end: 91
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1059300
display id: annotation1059300
version: 1
top level: BBa_I12040
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I12040/annotation1059300/range1059300/1

https://synbiohub.org/public/igem/BBa_I12040/annotation1059300/range1059300/1
type: Range
persistent identity: range1059300
display id: range1059300
version: 1
direction: direction/forward
top level: BBa_I12040
start: 48
end: 53
orientation: inline

OR1 lambda
type: SequenceAnnotation
persistent identity: annotation1059298
display id: annotation1059298
version: 1
top level: BBa_I12040
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12040/annotation1059298/range1059298/1

https://synbiohub.org/public/igem/BBa_I12040/annotation1059298/range1059298/1
type: Range
persistent identity: range1059298
display id: range1059298
version: 1
direction: direction/forward
top level: BBa_I12040
start: 9
end: 25
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1059302
display id: annotation1059302
version: 1
top level: BBa_I12040
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I12040/annotation1059302/range1059302/1

https://synbiohub.org/public/igem/BBa_I12040/annotation1059302/range1059302/1
type: Range
persistent identity: range1059302
display id: range1059302
version: 1
direction: direction/forward
top level: BBa_I12040
start: 71
end: 76
orientation: inline

NAND2-7
type: ComponentDefinition
persistent identity: BBa I732304
display id: BBa_I732304
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NAND Candidate (U073O22D001O47)
creator: Ma Rui
created: 2007-10-02T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1592
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: U97NUL D001O44
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732304_sequence/1

BBa_S03385
type: ComponentDefinition
persistent identity: BBa S03385
display id: BBa_S03385
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cold-sensing promoter (hybB)
creator: Elizabeth Clarke
created: 2005-11-03T12:00:00Z
modified: 2015-05-08T01:14:22Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _9_
m user id: 0
owner id: 473
owning group id: 9
sample status: No part sequence
status: status/Planning
bookmark: false
mutable description: This is the hybB promoter, which normally controlls hydrogenase regulation at low temperatures. It turns ON at 30oC and is active as low as 20oC (lower not tested). It is OFF at 37 and higher temperatures. In the literature, it is also active in anaerobic environments. This hasn't been tested.
mutable provenance: lizjaneclarke@gmail.com Univerity of California - San Francisco
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory

BBa_K1486015
type: ComponentDefinition
persistent identity: BBa K1486015
display id: BBa_K1486015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IFP[2]-CpxR under pBAD
creator: EPFL iGem team 2014
created: 2014-09-09T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part has a fused protein downstream of an arabinose-inducible promoter: IFP[2]-CpxR. Biobrick BBa_K1486014 has IFP[1]-CpxR with an arabinose promoter. This construct was made in both orientations: BBa_K1486013 is CpxR-IFP[2] (with an arabinose promoter).
mutable notes: Both orientations were made, as well as for IFP[1] so all dimerizations could be tested (see main page for biobrick numbers). Flexible linkers were used to fuse both proteins, and an RBS was put before each one of them.
mutable provenance: To edit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1486007
component: BBa_B0034
component: BBa_K1486004
component: BBa_K1486000
component: BBa_I0500
sequence annotation: BBa_I0500
sequence annotation: BBa_B0034
sequence annotation: BBa_K1486000
sequence annotation: BBa_K1486007
sequence annotation: BBa_K1486004
sequence: https://synbiohub.org/public/igem/BBa_K1486015_sequence/1

BBa_K1486007
type: Component
persistent identity: component2382854
display id: component2382854
version: 1
top level: BBa_K1486015
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486007/1

BBa_B0034
type: Component
persistent identity: component2382850
display id: component2382850
version: 1
top level: BBa_K1486015
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486004
type: Component
persistent identity: component2382855
display id: component2382855
version: 1
top level: BBa_K1486015
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_K1486000
type: Component
persistent identity: component2382859
display id: component2382859
version: 1
top level: BBa_K1486015
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_I0500
type: Component
persistent identity: component2382848
display id: component2382848
version: 1
top level: BBa_K1486015
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2382848
display id: annotation2382848
version: 1
top level: BBa_K1486015
location: https://synbiohub.org/public/igem/BBa_K1486015/annotation2382848/range2382848/1
component: BBa_I0500

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type: Range
persistent identity: range2382848
display id: range2382848
version: 1
direction: direction/reverse
top level: BBa_K1486015
start: 1
end: 1210
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2382850
display id: annotation2382850
version: 1
top level: BBa_K1486015
location: https://synbiohub.org/public/igem/BBa_K1486015/annotation2382850/range2382850/1
component: BBa_B0034

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type: Range
persistent identity: range2382850
display id: range2382850
version: 1
direction: direction/reverse
top level: BBa_K1486015
start: 1219
end: 1230
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2382859
display id: annotation2382859
version: 1
top level: BBa_K1486015
location: https://synbiohub.org/public/igem/BBa_K1486015/annotation2382859/range2382859/1
component: BBa_K1486000

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type: Range
persistent identity: range2382859
display id: range2382859
version: 1
direction: direction/reverse
top level: BBa_K1486015
start: 1860
end: 2561
orientation: inline

BBa_K1486007
type: SequenceAnnotation
persistent identity: annotation2382854
display id: annotation2382854
version: 1
top level: BBa_K1486015
location: https://synbiohub.org/public/igem/BBa_K1486015/annotation2382854/range2382854/1
component: BBa_K1486007

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type: Range
persistent identity: range2382854
display id: range2382854
version: 1
direction: direction/reverse
top level: BBa_K1486015
start: 1237
end: 1815
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2382855
display id: annotation2382855
version: 1
top level: BBa_K1486015
location: https://synbiohub.org/public/igem/BBa_K1486015/annotation2382855/range2382855/1
component: BBa_K1486004

https://synbiohub.org/public/igem/BBa_K1486015/annotation2382855/range2382855/1
type: Range
persistent identity: range2382855
display id: range2382855
version: 1
direction: direction/reverse
top level: BBa_K1486015
start: 1824
end: 1853
orientation: inline

Las CIO
type: ComponentDefinition
persistent identity: BBa I14016
display id: BBa_I14016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: P(Las) CIO
creator: Vikram Vijayan, Allen Hsu, Lawrence Fomundam
created: 2004-07-29T11:00:00Z
modified: 2015-08-31T04:07:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _4_
m user id: 0
owner id: 171
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: LasR and 3OC12HSL regulated promoter with CI Operator 1 (binding site for lambda cI repressor)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: CI lambda O1
sequence annotation: -35
sequence annotation: R0079
sequence: https://synbiohub.org/public/igem/BBa_I14016_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation1026125
display id: annotation1026125
version: 1
top level: Las CIO
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I14016/annotation1026125/range1026125/1

https://synbiohub.org/public/igem/BBa_I14016/annotation1026125/range1026125/1
type: Range
persistent identity: range1026125
display id: range1026125
version: 1
direction: direction/forward
top level: Las CIO
start: 140
end: 145
orientation: inline

CI lambda O1
type: SequenceAnnotation
persistent identity: annotation1017878
display id: annotation1017878
version: 1
top level: Las CIO
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I14016/annotation1017878/range1017878/1

https://synbiohub.org/public/igem/BBa_I14016/annotation1017878/range1017878/1
type: Range
persistent identity: range1017878
display id: range1017878
version: 1
direction: direction/forward
top level: Las CIO
start: 152
end: 168
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1026124
display id: annotation1026124
version: 1
top level: Las CIO
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I14016/annotation1026124/range1026124/1

https://synbiohub.org/public/igem/BBa_I14016/annotation1026124/range1026124/1
type: Range
persistent identity: range1026124
display id: range1026124
version: 1
direction: direction/forward
top level: Las CIO
start: 117
end: 122
orientation: inline

R0079
type: SequenceAnnotation
persistent identity: annotation1017859
display id: annotation1017859
version: 1
top level: Las CIO
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I14016/annotation1017859/range1017859/1

https://synbiohub.org/public/igem/BBa_I14016/annotation1017859/range1017859/1
type: Range
persistent identity: range1017859
display id: range1017859
version: 1
direction: direction/forward
top level: Las CIO
start: 1
end: 151
orientation: inline

BBa_I766200
type: ComponentDefinition
persistent identity: BBa I766200
display id: BBa_I766200
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSte2
creator: Nili Sommovilla
created: 2007-10-21T11:00:00Z
modified: 2015-08-31T04:08:09Z
discontinued: false
dominant: false
group u list: _155_
m user id: 0
owner id: 1931
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: endogenous promoter of Ste2
mutable notes: none.
mutable provenance: from yeast genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I766200_sequence/1

BBa_K1486043
type: ComponentDefinition
persistent identity: BBa K1486043
display id: BBa_K1486043
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Leucine Zipper + split rLuc
creator: EPFL iGem team 2014
created: 2014-10-01T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: To edit. Pense ?? rajouter les linkers, je ne sais pas s'ils sont de igem ou s'il faut ajouter la s??quence ?? la registry. Check pour tous tes biobricks et dis-moi, please.
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_K1486042
component: BBa_K1486019
component: BBa_K1486020
component: BBa_I0500
sequence annotation: BBa_K1486020
sequence annotation: BBa_K1486042
sequence annotation: BBa_B0034
sequence annotation: BBa_K1486019
sequence annotation: BBa_I0500
sequence: https://synbiohub.org/public/igem/BBa_K1486043_sequence/1

BBa_B0034
type: Component
persistent identity: component2392176
display id: component2392176
version: 1
top level: BBa_K1486043
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: Component
persistent identity: component2392172
display id: component2392172
version: 1
top level: BBa_K1486043
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486042
type: Component
persistent identity: component2392177
display id: component2392177
version: 1
top level: BBa_K1486043
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486042/1

BBa_K1486019
type: Component
persistent identity: component2392174
display id: component2392174
version: 1
top level: BBa_K1486043
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486019/1

BBa_K1486042
type: Component
persistent identity: component2392173
display id: component2392173
version: 1
top level: BBa_K1486043
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486042/1

BBa_K1486020
type: Component
persistent identity: component2392178
display id: component2392178
version: 1
top level: BBa_K1486043
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486020/1

BBa_I0500
type: Component
persistent identity: component2392170
display id: component2392170
version: 1
top level: BBa_K1486043
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_K1486020
type: SequenceAnnotation
persistent identity: annotation2392178
display id: annotation2392178
version: 1
top level: BBa_K1486043
location: https://synbiohub.org/public/igem/BBa_K1486043/annotation2392178/range2392178/1
component: BBa_K1486020

https://synbiohub.org/public/igem/BBa_K1486043/annotation2392178/range2392178/1
type: Range
persistent identity: range2392178
display id: range2392178
version: 1
direction: direction/reverse
top level: BBa_K1486043
start: 1865
end: 2470
orientation: inline

BBa_K1486042
type: SequenceAnnotation
persistent identity: annotation2392173
display id: annotation2392173
version: 1
top level: BBa_K1486043
location: https://synbiohub.org/public/igem/BBa_K1486043/annotation2392173/range2392173/1
component: BBa_K1486042

https://synbiohub.org/public/igem/BBa_K1486043/annotation2392173/range2392173/1
type: Range
persistent identity: range2392173
display id: range2392173
version: 1
direction: direction/reverse
top level: BBa_K1486043
start: 1223
end: 1372
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2392176
display id: annotation2392176
version: 1
top level: BBa_K1486043
location: https://synbiohub.org/public/igem/BBa_K1486043/annotation2392176/range2392176/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486043/annotation2392176/range2392176/1
type: Range
persistent identity: range2392176
display id: range2392176
version: 1
direction: direction/reverse
top level: BBa_K1486043
start: 1703
end: 1714
orientation: inline

BBa_K1486019
type: SequenceAnnotation
persistent identity: annotation2392174
display id: annotation2392174
version: 1
top level: BBa_K1486043
location: https://synbiohub.org/public/igem/BBa_K1486043/annotation2392174/range2392174/1
component: BBa_K1486019

https://synbiohub.org/public/igem/BBa_K1486043/annotation2392174/range2392174/1
type: Range
persistent identity: range2392174
display id: range2392174
version: 1
direction: direction/reverse
top level: BBa_K1486043
start: 1373
end: 1702
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2392172
display id: annotation2392172
version: 1
top level: BBa_K1486043
location: https://synbiohub.org/public/igem/BBa_K1486043/annotation2392172/range2392172/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486043/annotation2392172/range2392172/1
type: Range
persistent identity: range2392172
display id: range2392172
version: 1
direction: direction/reverse
top level: BBa_K1486043
start: 1211
end: 1222
orientation: inline

BBa_K1486042
type: SequenceAnnotation
persistent identity: annotation2392177
display id: annotation2392177
version: 1
top level: BBa_K1486043
location: https://synbiohub.org/public/igem/BBa_K1486043/annotation2392177/range2392177/1
component: BBa_K1486042

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type: Range
persistent identity: range2392177
display id: range2392177
version: 1
direction: direction/reverse
top level: BBa_K1486043
start: 1715
end: 1864
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2392170
display id: annotation2392170
version: 1
top level: BBa_K1486043
location: https://synbiohub.org/public/igem/BBa_K1486043/annotation2392170/range2392170/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486043/annotation2392170/range2392170/1
type: Range
persistent identity: range2392170
display id: range2392170
version: 1
direction: direction/reverse
top level: BBa_K1486043
start: 1
end: 1210
orientation: inline

NOR2
type: ComponentDefinition
persistent identity: BBa I732352
display id: BBa_I732352
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NOR Candidate (U035O44D001O22)
creator: Zhan Jian
created: 2007-10-02T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _156_
m user id: 0
owner id: 1592
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: U97NUL D001O61
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732352_sequence/1

BBa_K993000
type: ComponentDefinition
persistent identity: BBa K993000
display id: BBa_K993000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RFP Generator with ROSE thermometer
creator: ABD??LKADİR KARADAĞ
created: 2013-06-13T11:00:00Z
modified: 2015-05-08T01:13:51Z
discontinued: false
dominant: false
group u list: _1290_
m user id: 0
owner id: 11156
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The repression of heat shock gene expression (ROSE) element is an RNA element found in the 5' UTR of some heat shock protein's mRNAs. The ROSE element is an RNA thermometer that negatively regulates heat shock gene expression. The secondary structure is thought to be altered by temperature, thus it is an RNA thermometer. This structure blocks access to the ribosome binding site at normal temperatures. During heat shock however, the structure changes freeing the ribosome binding site and allowing expression to occur. RNA Thermometers are the only known single-component regulators of gene expression.(1) RNA Thermometers are thermosensors that regulate gene expression by temperature-induced changes in RNA conformation. Naturally occurring RNA Thermometers exhibit complex secondary structures which are believed to undergo a series of gradual structural changes in response to temperature shifts.(2) RNA Thermometers often regulate genes required during either a heat shock or cold shock response, but have been implicated in other regulatory roles such as in pathogenecity and starvation.(3)
mutable notes: Note that RNA thermometer actually works as an RBS sequence. Therefore, this part does not include an additional ribosome binding site. Also, this part does not contain an terminator sequence, but a simple stop codon. The RFP should be translated continuously.
mutable provenance: Bradyrhizobium japonicum USDA 110 and Registry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: Finish Codon
sequence annotation: RFP Generator
sequence annotation: ROSE Thermometer
sequence annotation: Start Codon
sequence annotation: pVeg2
sequence: https://synbiohub.org/public/igem/BBa_K993000_sequence/1

Finish Codon
type: SequenceAnnotation
persistent identity: annotation2329456
display id: annotation2329456
version: 1
top level: BBa_K993000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K993000/annotation2329456/range2329456/1

https://synbiohub.org/public/igem/BBa_K993000/annotation2329456/range2329456/1
type: Range
persistent identity: range2329456
display id: range2329456
version: 1
direction: direction/forward
top level: BBa_K993000
start: 900
end: 902
orientation: inline

RFP Generator
type: SequenceAnnotation
persistent identity: annotation2329455
display id: annotation2329455
version: 1
top level: BBa_K993000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K993000/annotation2329455/range2329455/1

https://synbiohub.org/public/igem/BBa_K993000/annotation2329455/range2329455/1
type: Range
persistent identity: range2329455
display id: range2329455
version: 1
direction: direction/forward
top level: BBa_K993000
start: 194
end: 902
orientation: inline

ROSE Thermometer
type: SequenceAnnotation
persistent identity: annotation2329454
display id: annotation2329454
version: 1
top level: BBa_K993000
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K993000/annotation2329454/range2329454/1

https://synbiohub.org/public/igem/BBa_K993000/annotation2329454/range2329454/1
type: Range
persistent identity: range2329454
display id: range2329454
version: 1
direction: direction/forward
top level: BBa_K993000
start: 98
end: 193
orientation: inline

Start Codon
type: SequenceAnnotation
persistent identity: annotation2329457
display id: annotation2329457
version: 1
top level: BBa_K993000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K993000/annotation2329457/range2329457/1

https://synbiohub.org/public/igem/BBa_K993000/annotation2329457/range2329457/1
type: Range
persistent identity: range2329457
display id: range2329457
version: 1
direction: direction/forward
top level: BBa_K993000
start: 194
end: 196
orientation: inline

pVeg2
type: SequenceAnnotation
persistent identity: annotation2329453
display id: annotation2329453
version: 1
top level: BBa_K993000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K993000/annotation2329453/range2329453/1

https://synbiohub.org/public/igem/BBa_K993000/annotation2329453/range2329453/1
type: Range
persistent identity: range2329453
display id: range2329453
version: 1
direction: direction/forward
top level: BBa_K993000
start: 1
end: 97
orientation: inline

U037O11+D0
type: ComponentDefinition
persistent identity: BBa I732443
display id: BBa_I732443
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U037O11+D002O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732443_sequence/1

U097NUL+D0
type: ComponentDefinition
persistent identity: BBa I732412
display id: BBa_I732412
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U097NUL+D062O22)
creator: Zhan Jian
created: 2007-09-22T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1572
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To be later edited if time permits.
mutable notes: To be later edited if time permits.
mutable provenance: To be later edited if time permits.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: consensus elements
sequence annotation: +1
sequence annotation: O2
sequence: https://synbiohub.org/public/igem/BBa_I732412_sequence/1

consensus elements
type: SequenceAnnotation
persistent identity: annotation1944564
display id: annotation1944564
version: 1
top level: U097NUL+D0
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I732412/annotation1944564/range1944564/1

https://synbiohub.org/public/igem/BBa_I732412/annotation1944564/range1944564/1
type: Range
persistent identity: range1944564
display id: range1944564
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 61
end: 137
orientation: inline

+1
type: SequenceAnnotation
persistent identity: annotation1944566
display id: annotation1944566
version: 1
top level: U097NUL+D0
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I732412/annotation1944566/range1944566/1

https://synbiohub.org/public/igem/BBa_I732412/annotation1944566/range1944566/1
type: Range
persistent identity: range1944566
display id: range1944566
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 137
end: 137
orientation: inline

O2
type: SequenceAnnotation
persistent identity: annotation1944565
display id: annotation1944565
version: 1
top level: U097NUL+D0
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I732412/annotation1944565/range1944565/1

https://synbiohub.org/public/igem/BBa_I732412/annotation1944565/range1944565/1
type: Range
persistent identity: range1944565
display id: range1944565
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 158
end: 177
orientation: inline

BBa_K145150
type: ComponentDefinition
persistent identity: BBa K145150
display id: BBa_K145150
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Hybrid promoter: HSL-LuxR activated, P22 C2 repressed
creator: Jonas Demeulemeester
created: 2008-08-05T11:00:00Z
modified: 2015-05-08T01:10:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _257_
m user id: 0
owner id: 2970
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Hybrid promoter consisting of the Lux box which overlaps partly with -35 box. Binding sites for P22 C2 OR2 and OR1 are located between the -35 and -10 boxes and downstream of them respectively.
mutable notes: -35 box is partly composite. -10 is taken from the P22 phage PR regulatory region
mutable provenance: Synthetic
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: OR2
sequence annotation: -35
sequence annotation: Lux-box
sequence annotation: OR1
sequence: https://synbiohub.org/public/igem/BBa_K145150_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation1971585
display id: annotation1971585
version: 1
top level: BBa_K145150
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K145150/annotation1971585/range1971585/1

https://synbiohub.org/public/igem/BBa_K145150/annotation1971585/range1971585/1
type: Range
persistent identity: range1971585
display id: range1971585
version: 1
direction: direction/forward
top level: BBa_K145150
start: 42
end: 47
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation1971584
display id: annotation1971584
version: 1
top level: BBa_K145150
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K145150/annotation1971584/range1971584/1

https://synbiohub.org/public/igem/BBa_K145150/annotation1971584/range1971584/1
type: Range
persistent identity: range1971584
display id: range1971584
version: 1
direction: direction/forward
top level: BBa_K145150
start: 23
end: 40
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1971583
display id: annotation1971583
version: 1
top level: BBa_K145150
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K145150/annotation1971583/range1971583/1

https://synbiohub.org/public/igem/BBa_K145150/annotation1971583/range1971583/1
type: Range
persistent identity: range1971583
display id: range1971583
version: 1
direction: direction/forward
top level: BBa_K145150
start: 20
end: 25
orientation: inline

Lux-box
type: SequenceAnnotation
persistent identity: annotation1971582
display id: annotation1971582
version: 1
top level: BBa_K145150
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K145150/annotation1971582/range1971582/1

https://synbiohub.org/public/igem/BBa_K145150/annotation1971582/range1971582/1
type: Range
persistent identity: range1971582
display id: range1971582
version: 1
direction: direction/forward
top level: BBa_K145150
start: 1
end: 20
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation1971586
display id: annotation1971586
version: 1
top level: BBa_K145150
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K145150/annotation1971586/range1971586/1

https://synbiohub.org/public/igem/BBa_K145150/annotation1971586/range1971586/1
type: Range
persistent identity: range1971586
display id: range1971586
version: 1
direction: direction/forward
top level: BBa_K145150
start: 46
end: 63
orientation: inline

BBa_K112405
type: ComponentDefinition
persistent identity: BBa K112405
display id: BBa_K112405
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter for CadA and CadB genes
creator: Christina Brown
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:17Z
discontinued: false
dominant: false
group u list: _224_
m user id: 0
owner id: 3241
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Our microarray experiment showed that this gene was responsive to sound and ultrasound. Therefore, we cloned the promoter region located approximately 250 base pairs before the start codon for the cadB gene.
mutable notes: There was a relatively high basal level of expression of this gene that made this part difficult to incorporate into our lysis device design.
mutable provenance: cloned from E. coli MG1655 genome located upstream of CadA and CadB genes
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K112405_sequence/1

BBa_I731004
type: ComponentDefinition
persistent identity: BBa I731004
display id: BBa_I731004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FecA promoter
creator: Shaunak Vankudre
created: 2007-06-25T11:00:00Z
modified: 2015-08-31T04:07:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _127_
m user id: 0
owner id: 1889
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: derived from Enz et al, 2003, J Bacteriol. 2003 April; 185(7): 2387???2391. doi: 10.1128/JB.185.7.2387-2391.2003.
mutable provenance: genomic sequence from E. Coli K12
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I731004_sequence/1

NAND59
type: ComponentDefinition
persistent identity: BBa I732305
display id: BBa_I732305
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NAND Candidate (U073O22D059O46)
creator: Ma Rui
created: 2007-10-02T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1592
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: U97NUL D001O55
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732305_sequence/1

BBa_K116500
type: ComponentDefinition
persistent identity: BBa K116500
display id: BBa_K116500
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: OmpF promoter that is activated or repressesed by OmpR according to osmolarity.
creator: Chun-Ju Yang
created: 2008-10-28T12:00:00Z
modified: 2015-05-08T01:09:33Z
discontinued: false
dominant: false
group u list: _210_
m user id: 0
owner id: 2083
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Promoter OmpF is activated by phosphorylated OmpR at low osmolarity, and is repressesed by phosphorylated OmpR at high osmolarity.In Escherichia coli, osmoregulation is mediated in part by the actions of such a two-component system consisting of EnvZ and OmpR. These proteins act to control the relative levels of the outer membrane porin genes, ompF and ompC. At low osmo larity, OmpF predominates in the outer membrane, while at high osmolarity the OmpF porin is replaced by OmpC.OmpF has a larger pore and a faster ??ow rate than OmpC. Reference: K. Mattison, R. Oropeza, N. Byers, L. J. Kenney, J Mol Biol 315, 497 (Jan 25, 2002).
mutable notes: The osmolarity range of OmpF sensing still have to be confirmed
mutable provenance: Promoter OmpF is activated by phosphorylated OmpR at low osmolarity, and is repressesed by phosphorylated OmpR at high osmolarity.In Escherichia coli, osmoregulation is mediated in part by the actions of such a two-component system consisting of EnvZ and OmpR. These proteins act to control the relative levels of the outer membrane porin genes, ompF and ompC. At low osmo larity, OmpF predominates in the outer membrane, while at high osmolarity the OmpF porin is replaced by OmpC.OmpF has a larger pore and a faster ??ow rate than OmpC.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OmpR binding
sequence: https://synbiohub.org/public/igem/BBa_K116500_sequence/1

OmpR binding
type: SequenceAnnotation
persistent identity: annotation1994040
display id: annotation1994040
version: 1
top level: BBa_K116500
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K116500/annotation1994040/range1994040/1

https://synbiohub.org/public/igem/BBa_K116500/annotation1994040/range1994040/1
type: Range
persistent identity: range1994040
display id: range1994040
version: 1
direction: direction/forward
top level: BBa_K116500
start: 27
end: 88
orientation: inline

U097NUL+D0
type: ComponentDefinition
persistent identity: BBa I732411
display id: BBa_I732411
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U097NUL+D050O22)
creator: Zhan Jian
created: 2007-09-22T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1572
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To be later edited if time permits.
mutable notes: To be later edited if time permits.
mutable provenance: To be later edited if time permits.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: +1
sequence annotation: O2
sequence annotation: consensus elements
sequence: https://synbiohub.org/public/igem/BBa_I732411_sequence/1

+1
type: SequenceAnnotation
persistent identity: annotation1944563
display id: annotation1944563
version: 1
top level: U097NUL+D0
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I732411/annotation1944563/range1944563/1

https://synbiohub.org/public/igem/BBa_I732411/annotation1944563/range1944563/1
type: Range
persistent identity: range1944563
display id: range1944563
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 137
end: 137
orientation: inline

O2
type: SequenceAnnotation
persistent identity: annotation1944562
display id: annotation1944562
version: 1
top level: U097NUL+D0
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I732411/annotation1944562/range1944562/1

https://synbiohub.org/public/igem/BBa_I732411/annotation1944562/range1944562/1
type: Range
persistent identity: range1944562
display id: range1944562
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 146
end: 165
orientation: inline

consensus elements
type: SequenceAnnotation
persistent identity: annotation1944561
display id: annotation1944561
version: 1
top level: U097NUL+D0
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I732411/annotation1944561/range1944561/1

https://synbiohub.org/public/igem/BBa_I732411/annotation1944561/range1944561/1
type: Range
persistent identity: range1944561
display id: range1944561
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 61
end: 137
orientation: inline

U037O11+D0
type: ComponentDefinition
persistent identity: BBa I732445
display id: BBa_I732445
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U037O11+D026O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732445_sequence/1

BBa_J05210
type: ComponentDefinition
persistent identity: BBa J05210
display id: BBa_J05210
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Modifed Prm+ Promoter
creator: Alexander Roth
created: 2005-09-05T11:00:00Z
modified: 2015-08-31T04:08:15Z
discontinued: false
dominant: false
group u list: _34_
m user id: 0
owner id: 412
owning group id: 34
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is identical to BBa_I12007 exept that some base has been modifed to allow for additional ZFP repressors to bind.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: -10
sequence annotation: OR2
sequence annotation: ZFP binding sites
sequence annotation: OR1
sequence: https://synbiohub.org/public/igem/BBa_J05210_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation1689959
display id: annotation1689959
version: 1
top level: BBa_J05210
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J05210/annotation1689959/range1689959/1

https://synbiohub.org/public/igem/BBa_J05210/annotation1689959/range1689959/1
type: Range
persistent identity: range1689959
display id: range1689959
version: 1
direction: direction/forward
top level: BBa_J05210
start: 48
end: 53
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1689961
display id: annotation1689961
version: 1
top level: BBa_J05210
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J05210/annotation1689961/range1689961/1

https://synbiohub.org/public/igem/BBa_J05210/annotation1689961/range1689961/1
type: Range
persistent identity: range1689961
display id: range1689961
version: 1
direction: direction/forward
top level: BBa_J05210
start: 71
end: 76
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation1689958
display id: annotation1689958
version: 1
top level: BBa_J05210
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J05210/annotation1689958/range1689958/1

https://synbiohub.org/public/igem/BBa_J05210/annotation1689958/range1689958/1
type: Range
persistent identity: range1689958
display id: range1689958
version: 1
direction: direction/forward
top level: BBa_J05210
start: 33
end: 49
orientation: inline

ZFP binding sites
type: SequenceAnnotation
persistent identity: annotation1689960
display id: annotation1689960
version: 1
top level: BBa_J05210
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J05210/annotation1689960/range1689960/1

https://synbiohub.org/public/igem/BBa_J05210/annotation1689960/range1689960/1
type: Range
persistent identity: range1689960
display id: range1689960
version: 1
direction: direction/forward
top level: BBa_J05210
start: 55
end: 66
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation1689957
display id: annotation1689957
version: 1
top level: BBa_J05210
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J05210/annotation1689957/range1689957/1

https://synbiohub.org/public/igem/BBa_J05210/annotation1689957/range1689957/1
type: Range
persistent identity: range1689957
display id: range1689957
version: 1
direction: direction/forward
top level: BBa_J05210
start: 9
end: 25
orientation: inline

BBa_K993003
type: ComponentDefinition
persistent identity: BBa K993003
display id: BBa_K993003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Urease generator with ROSE thermometer
creator: FURKAN SACİT CEYLAN
created: 2013-06-13T11:00:00Z
modified: 2015-05-08T01:13:51Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1290_
m user id: 0
owner id: 11155
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Repression of heat shock gene expression (ROSE) element The repression of heat shock gene expression (ROSE) element is an RNA element found in the 5' UTR of some heat shock protein's mRNAs. The ROSE element is an RNA thermometer that negatively regulates heat shock gene expression. The secondary structure is thought to be altered by temperature, thus it is an RNA thermometer. This structure blocks access to the ribosome binding site at normal temperatures. During heat shock however, the structure changes freeing the ribosome binding site and allowing expression to occur.[2][3] A partial structure of this RNA element has been determined using NMR.[1] ROSE1 and ROSEAT2 are specific examples of ROSE elements.[4] ROSE1 is found in Bradyrhizobium japonicum whereas ROSEAT2 is a closely related element from Agrobacterium tumefaciens. The two RNA elements have similar secondary structures with ROSE1 having an extra hairpin.[4] All ROSE elements contain a characteristic 'bulged G' opposite the Shine-Dalgarno sequence binding site, without this nucleotide the RNA thermometer loses its temperature-sensitivity.[5]
mutable notes: Note that RNA thermometer actually works as an RBS sequence. Therefore, this part does not include an additional ribosome binding site. Also, this part does not contain an terminator sequence, but a simple stop codon. The RFP should be translated continuously.
mutable provenance: AATTTTGTCAAAATAATTTTATTGACAACGTCTTATTAACGTTGATATAATTTAAATTTTTTTTGACAAAAATGGGCTCGTGTTGTACAATAAATGTGCCGCGACAAGCGGTCCGGGCGCCCTAGGGGCCCGGCGGAGACGGGCGCCGGAGGTGTCCGACGCCTGCTCGTCAAGTTCTTGCTCCTTGGAGGATATGAAGATAAACAGGCAGCAGTACGCGGAGAGCTACGGGCCGACGGTGGGGGACAGGGTGAGGCTGGCGGACACGGACCTGGGGGAGGTGGAGAAGGACTACTACTACCTGGGGGACGAGGTGAACTTCGGGGGGGGGAAGGTGCTGAGGGAGGGGATGGGGGAGAACGGGACGTACACGAGGACGGAGAACGTGTTGGACCTGCTGCTGACGAACGCGCTGATATTGGACTACACGGGGATATACAAGGCGGACATAGGGGTGAAGGACGGGTACATAGTGGGGATAGGGAAGGGGGGGAACCCGGACATAATGGACGGGGTGACGCCGAACATGATAGTGGGGACGGCGACGGAGGTGATAGCGGCGGAGGGGAAGATAGTGACGGCGGGGGGGATAGACACGCACGTGCACTTCATAAACCCGGACCAGGTGGACGTGGCGCTGGCGAACGGGATAACGACGCTGTTCGGGGGGGGGACGGGGCCGGCGGAGGGGAGCAAGGCGACGACGGTGACGCCGGGGCCGTGGAACATAGAGAAGATGCTGAAGAGCACGGAGGGGCTGCCGATAAACGTGGGGATACTGGGGAAGGGGCACGGGAGCAGCATAGCGCCGATAATGGAGCAGATAGACGCGGGGGCGGCGGGGCTGAAGATACACGAGGACTGGGGGGCGACGCCGGCGAGCATAGACAGGAGCCTGACGGTGGCGGACGAGGCGGACGTGCAGGTGGCGATACACAGCGACACGCTGAACGAGGCGGGGTTCTTGGAGGACACGGTGAGGGCGATAAACGGGAGGGTGATACACAGCTTCCACGTGGAGGGGGCGGGGGGGGGGCACGCGCCGGACATAATGGCGATGGCGGGGCACCCGAACGTGCTGCCGAGCAGCACGAACCCGACGAGGCCGTTCACGGTGAACACGATAGACGAGCACTTGGACATGCTGATGGTGTGCCACCACCTGAAGCAGAACATACCGGAGGACGTGGCGTTCGCGGACAGCAGGATAAGGCCGGAGACGATAGCGGCGGAGGACATACTGCACGACCTGGGGATAATAAGCATGATGAGCACGGACGCGCTGGCGATGGGGAGGGCGGGGGAGATGGTGCTGAGGACGTGGCAGACGGCGGACAAGATGAAGAAGCAGAGGGGGCCGCTGGCGGAGGAGAAGAACGGGAGCGACAACTTCAGGCTGAAGAGGTACGTGAGCAAGTACACGATAAACCCGGCGATAGCGCAGGGGATGGCGCACGAGGTGGGGAGCATAGAGGAGGGGAAGTTCGCGGACCTGGTGCTGTGGGAGCCGAAGTTCTTCGGGGTGAAGGCGGACAGGGTGATAAAGGGGGGGATAATAGCGTACGCGCAGATAGGGGACCCGAGCGCGAGCATACCGACGCCGCAGCCGGTGATGGGGAGGAGGATGTACGGGACGGTGGGGGACCTGATACACGACACGAACATAACGTTCATGAGCAAGAGCAGCATACAGCAGGGGGTGCCGGCGAAGCTGGGGCTGAAGAGGAGGATAGGGACGGTGAAGAATTGTCGAAACATAGGGAAGAAGGACATGAAGTGGAACGACGTGACGACGGACATAGACATAAACCCGGAGACGTACGAGGTGAAGGTGGACGGGGAGGTGCTGACGTGCGAGCCGGTGAAGGAGCTGCCGATGGCGCAGAGGTACTTCCTGTTCTAATCGCTGGGACGCCCGCCCCTAGAAAAGGAGGACTAAAAATGAGCAACAACAACTACATAGTGCCGGGGGAGTACAGGGTGGCGGAGGGGGAGATAGA…
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: RBS
sequence annotation: Insulator
sequence annotation: Urease A
sequence annotation: Urease C
sequence annotation: Start Codon
sequence annotation: Stop Codon
sequence annotation: Urease B
sequence annotation: pVeg2
sequence annotation: ROSE Thermometer
sequence: https://synbiohub.org/public/igem/BBa_K993003_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2329461
display id: annotation2329461
version: 1
top level: BBa_K993003
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K993003/annotation2329461/range2329461/1

https://synbiohub.org/public/igem/BBa_K993003/annotation2329461/range2329461/1
type: Range
persistent identity: range2329461
display id: range2329461
version: 1
direction: direction/forward
top level: BBa_K993003
start: 1919
end: 1941
orientation: inline

Insulator
type: SequenceAnnotation
persistent identity: annotation2329464
display id: annotation2329464
version: 1
top level: BBa_K993003
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K993003/annotation2329464/range2329464/1

https://synbiohub.org/public/igem/BBa_K993003/annotation2329464/range2329464/1
type: Range
persistent identity: range2329464
display id: range2329464
version: 1
direction: direction/forward
top level: BBa_K993003
start: 2323
end: 2337
orientation: inline

Urease A
type: SequenceAnnotation
persistent identity: annotation2329459
display id: annotation2329459
version: 1
top level: BBa_K993003
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K993003/annotation2329459/range2329459/1

https://synbiohub.org/public/igem/BBa_K993003/annotation2329459/range2329459/1
type: Range
persistent identity: range2329459
display id: range2329459
version: 1
direction: direction/forward
top level: BBa_K993003
start: 194
end: 1903
orientation: inline

Insulator
type: SequenceAnnotation
persistent identity: annotation2329460
display id: annotation2329460
version: 1
top level: BBa_K993003
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K993003/annotation2329460/range2329460/1

https://synbiohub.org/public/igem/BBa_K993003/annotation2329460/range2329460/1
type: Range
persistent identity: range2329460
display id: range2329460
version: 1
direction: direction/forward
top level: BBa_K993003
start: 1904
end: 1918
orientation: inline

Urease C
type: SequenceAnnotation
persistent identity: annotation2329466
display id: annotation2329466
version: 1
top level: BBa_K993003
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K993003/annotation2329466/range2329466/1

https://synbiohub.org/public/igem/BBa_K993003/annotation2329466/range2329466/1
type: Range
persistent identity: range2329466
display id: range2329466
version: 1
direction: direction/forward
top level: BBa_K993003
start: 2361
end: 2663
orientation: inline

Start Codon
type: SequenceAnnotation
persistent identity: annotation2329462
display id: annotation2329462
version: 1
top level: BBa_K993003
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K993003/annotation2329462/range2329462/1

https://synbiohub.org/public/igem/BBa_K993003/annotation2329462/range2329462/1
type: Range
persistent identity: range2329462
display id: range2329462
version: 1
direction: direction/forward
top level: BBa_K993003
start: 194
end: 196
orientation: inline

Stop Codon
type: SequenceAnnotation
persistent identity: annotation2329470
display id: annotation2329470
version: 1
top level: BBa_K993003
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K993003/annotation2329470/range2329470/1

https://synbiohub.org/public/igem/BBa_K993003/annotation2329470/range2329470/1
type: Range
persistent identity: range2329470
display id: range2329470
version: 1
direction: direction/forward
top level: BBa_K993003
start: 2320
end: 2322
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2329465
display id: annotation2329465
version: 1
top level: BBa_K993003
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K993003/annotation2329465/range2329465/1

https://synbiohub.org/public/igem/BBa_K993003/annotation2329465/range2329465/1
type: Range
persistent identity: range2329465
display id: range2329465
version: 1
direction: direction/forward
top level: BBa_K993003
start: 2338
end: 2360
orientation: inline

Urease B
type: SequenceAnnotation
persistent identity: annotation2329463
display id: annotation2329463
version: 1
top level: BBa_K993003
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K993003/annotation2329463/range2329463/1

https://synbiohub.org/public/igem/BBa_K993003/annotation2329463/range2329463/1
type: Range
persistent identity: range2329463
display id: range2329463
version: 1
direction: direction/forward
top level: BBa_K993003
start: 1942
end: 2322
orientation: inline

Start Codon
type: SequenceAnnotation
persistent identity: annotation2329468
display id: annotation2329468
version: 1
top level: BBa_K993003
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K993003/annotation2329468/range2329468/1

https://synbiohub.org/public/igem/BBa_K993003/annotation2329468/range2329468/1
type: Range
persistent identity: range2329468
display id: range2329468
version: 1
direction: direction/forward
top level: BBa_K993003
start: 2361
end: 2363
orientation: inline

pVeg2
type: SequenceAnnotation
persistent identity: annotation2329447
display id: annotation2329447
version: 1
top level: BBa_K993003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K993003/annotation2329447/range2329447/1

https://synbiohub.org/public/igem/BBa_K993003/annotation2329447/range2329447/1
type: Range
persistent identity: range2329447
display id: range2329447
version: 1
direction: direction/forward
top level: BBa_K993003
start: 1
end: 97
orientation: inline

Start Codon
type: SequenceAnnotation
persistent identity: annotation2329467
display id: annotation2329467
version: 1
top level: BBa_K993003
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K993003/annotation2329467/range2329467/1

https://synbiohub.org/public/igem/BBa_K993003/annotation2329467/range2329467/1
type: Range
persistent identity: range2329467
display id: range2329467
version: 1
direction: direction/forward
top level: BBa_K993003
start: 1942
end: 1944
orientation: inline

Stop Codon
type: SequenceAnnotation
persistent identity: annotation2329471
display id: annotation2329471
version: 1
top level: BBa_K993003
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K993003/annotation2329471/range2329471/1

https://synbiohub.org/public/igem/BBa_K993003/annotation2329471/range2329471/1
type: Range
persistent identity: range2329471
display id: range2329471
version: 1
direction: direction/forward
top level: BBa_K993003
start: 2661
end: 2663
orientation: inline

Stop Codon
type: SequenceAnnotation
persistent identity: annotation2329469
display id: annotation2329469
version: 1
top level: BBa_K993003
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K993003/annotation2329469/range2329469/1

https://synbiohub.org/public/igem/BBa_K993003/annotation2329469/range2329469/1
type: Range
persistent identity: range2329469
display id: range2329469
version: 1
direction: direction/forward
top level: BBa_K993003
start: 1901
end: 1903
orientation: inline

ROSE Thermometer
type: SequenceAnnotation
persistent identity: annotation2329458
display id: annotation2329458
version: 1
top level: BBa_K993003
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K993003/annotation2329458/range2329458/1

https://synbiohub.org/public/igem/BBa_K993003/annotation2329458/range2329458/1
type: Range
persistent identity: range2329458
display id: range2329458
version: 1
direction: direction/forward
top level: BBa_K993003
start: 98
end: 193
orientation: inline

BBa_K1486054
type: ComponentDefinition
persistent identity: BBa K1486054
display id: BBa_K1486054
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CheY/CheZ + split rLuc
creator: EPFL iGem team 2014
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: to edit
mutable notes: to edit
mutable provenance: to edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_I0500
component: BBa_K1486053
component: BBa_B0034
component: BBa_K1486020
component: BBa_K1486019
component: BBa_K569017
component: BBa_K629003
sequence annotation: BBa_K1486020
sequence annotation: BBa_K1486053
sequence annotation: BBa_B0034
sequence annotation: BBa_K1486019
sequence annotation: BBa_I0500
sequence annotation: BBa_K569017
sequence annotation: BBa_K629003
sequence: https://synbiohub.org/public/igem/BBa_K1486054_sequence/1

BBa_I0500
type: Component
persistent identity: component2399859
display id: component2399859
version: 1
top level: BBa_K1486054
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_K1486053
type: Component
persistent identity: component2399863
display id: component2399863
version: 1
top level: BBa_K1486054
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486053/1

BBa_B0034
type: Component
persistent identity: component2399861
display id: component2399861
version: 1
top level: BBa_K1486054
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486020
type: Component
persistent identity: component2399875
display id: component2399875
version: 1
top level: BBa_K1486054
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486020/1

BBa_K1486019
type: Component
persistent identity: component2399864
display id: component2399864
version: 1
top level: BBa_K1486054
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486019/1

BBa_B0034
type: Component
persistent identity: component2399866
display id: component2399866
version: 1
top level: BBa_K1486054
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K569017
type: Component
persistent identity: component2399862
display id: component2399862
version: 1
top level: BBa_K1486054
access: public
definition: https://synbiohub.org/public/igem/BBa_K569017/1

BBa_K629003
type: Component
persistent identity: component2399873
display id: component2399873
version: 1
top level: BBa_K1486054
access: public
definition: https://synbiohub.org/public/igem/BBa_K629003/1

BBa_K1486053
type: Component
persistent identity: component2399874
display id: component2399874
version: 1
top level: BBa_K1486054
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486053/1

BBa_K1486020
type: SequenceAnnotation
persistent identity: annotation2399875
display id: annotation2399875
version: 1
top level: BBa_K1486054
location: https://synbiohub.org/public/igem/BBa_K1486054/annotation2399875/range2399875/1
component: BBa_K1486020

https://synbiohub.org/public/igem/BBa_K1486054/annotation2399875/range2399875/1
type: Range
persistent identity: range2399875
display id: range2399875
version: 1
direction: direction/reverse
top level: BBa_K1486054
start: 2688
end: 3293
orientation: inline

BBa_K1486053
type: SequenceAnnotation
persistent identity: annotation2399874
display id: annotation2399874
version: 1
top level: BBa_K1486054
location: https://synbiohub.org/public/igem/BBa_K1486054/annotation2399874/range2399874/1
component: BBa_K1486053

https://synbiohub.org/public/igem/BBa_K1486054/annotation2399874/range2399874/1
type: Range
persistent identity: range2399874
display id: range2399874
version: 1
direction: direction/reverse
top level: BBa_K1486054
start: 2658
end: 2687
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2399861
display id: annotation2399861
version: 1
top level: BBa_K1486054
location: https://synbiohub.org/public/igem/BBa_K1486054/annotation2399861/range2399861/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486054/annotation2399861/range2399861/1
type: Range
persistent identity: range2399861
display id: range2399861
version: 1
direction: direction/reverse
top level: BBa_K1486054
start: 1211
end: 1222
orientation: inline

BBa_K1486053
type: SequenceAnnotation
persistent identity: annotation2399863
display id: annotation2399863
version: 1
top level: BBa_K1486054
location: https://synbiohub.org/public/igem/BBa_K1486054/annotation2399863/range2399863/1
component: BBa_K1486053

https://synbiohub.org/public/igem/BBa_K1486054/annotation2399863/range2399863/1
type: Range
persistent identity: range2399863
display id: range2399863
version: 1
direction: direction/reverse
top level: BBa_K1486054
start: 1641
end: 1670
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2399866
display id: annotation2399866
version: 1
top level: BBa_K1486054
location: https://synbiohub.org/public/igem/BBa_K1486054/annotation2399866/range2399866/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486054/annotation2399866/range2399866/1
type: Range
persistent identity: range2399866
display id: range2399866
version: 1
direction: direction/reverse
top level: BBa_K1486054
start: 2001
end: 2012
orientation: inline

BBa_K1486019
type: SequenceAnnotation
persistent identity: annotation2399864
display id: annotation2399864
version: 1
top level: BBa_K1486054
location: https://synbiohub.org/public/igem/BBa_K1486054/annotation2399864/range2399864/1
component: BBa_K1486019

https://synbiohub.org/public/igem/BBa_K1486054/annotation2399864/range2399864/1
type: Range
persistent identity: range2399864
display id: range2399864
version: 1
direction: direction/reverse
top level: BBa_K1486054
start: 1671
end: 2000
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2399859
display id: annotation2399859
version: 1
top level: BBa_K1486054
location: https://synbiohub.org/public/igem/BBa_K1486054/annotation2399859/range2399859/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486054/annotation2399859/range2399859/1
type: Range
persistent identity: range2399859
display id: range2399859
version: 1
direction: direction/reverse
top level: BBa_K1486054
start: 1
end: 1210
orientation: inline

BBa_K569017
type: SequenceAnnotation
persistent identity: annotation2399862
display id: annotation2399862
version: 1
top level: BBa_K1486054
location: https://synbiohub.org/public/igem/BBa_K1486054/annotation2399862/range2399862/1
component: BBa_K569017

https://synbiohub.org/public/igem/BBa_K1486054/annotation2399862/range2399862/1
type: Range
persistent identity: range2399862
display id: range2399862
version: 1
direction: direction/reverse
top level: BBa_K1486054
start: 1223
end: 1640
orientation: inline

BBa_K629003
type: SequenceAnnotation
persistent identity: annotation2399873
display id: annotation2399873
version: 1
top level: BBa_K1486054
location: https://synbiohub.org/public/igem/BBa_K1486054/annotation2399873/range2399873/1
component: BBa_K629003

https://synbiohub.org/public/igem/BBa_K1486054/annotation2399873/range2399873/1
type: Range
persistent identity: range2399873
display id: range2399873
version: 1
direction: direction/reverse
top level: BBa_K1486054
start: 2013
end: 2657
orientation: inline

glnAp2 pro
type: ComponentDefinition
persistent identity: BBa J64979
display id: BBa_J64979
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: glnAp2
creator: Kristopher Kuchenbecker
created: 2007-03-25T12:00:00Z
modified: 2015-05-08T01:08:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 1429
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This promoter sequence (-80 to +1) is responsive to the transcription enhancer glnG.
mutable notes: n/a
mutable provenance: ''E. coli''
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J64979_sequence/1

U037O11+D0
type: ComponentDefinition
persistent identity: BBa I732444
display id: BBa_I732444
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U037O11+D014O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732444_sequence/1

U085O11+D0
type: ComponentDefinition
persistent identity: BBa I732420
display id: BBa_I732420
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U085O11+D014O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732420_sequence/1

cI+luxR
type: ComponentDefinition
persistent identity: BBa R0065
display id: BBa_R0065
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (lambda cI and luxR regulated -- hybrid)
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: cI repressor negatively regulates this promoter and LuxR activates its transcription.The effect of cI is dominant over LuxR. This part is based on the LuxR and cI repressor regulated hybrid promoter designed and tested by Ron Weiss. It requires the binding of two cI repressor dimers for maximal repression and contains two cI repressor binding sites namely, OR1 and OR2. This promoter is leaky in the sense that 'some' transcription is seen in the absence of both cI and LuxR. LuxI cI activity of promoter + + zero + - maximum - + zero - - leaky (no quantitative information)
mutable notes: This part was designed based on the LuxR and cI repressor regulated hybrid promoter tested by Ron Weiss and the LuxR-LuxICDABE sequence annotated by Tom Knight AF170104 .
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Lux Box
sequence annotation: -10
sequence annotation: -35
sequence annotation: OR1 cI
sequence annotation: BBa_R0065
sequence annotation: lux p(R) start
sequence annotation: OR2 cI
sequence: https://synbiohub.org/public/igem/BBa_R0065_sequence/1

Lux Box
type: SequenceAnnotation
persistent identity: annotation1986775
display id: annotation1986775
version: 1
top level: cI+luxR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986775/range1986775/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986775/range1986775/1
type: Range
persistent identity: range1986775
display id: range1986775
version: 1
direction: direction/forward
top level: cI+luxR
start: 6
end: 25
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986781
display id: annotation1986781
version: 1
top level: cI+luxR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986781/range1986781/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986781/range1986781/1
type: Range
persistent identity: range1986781
display id: range1986781
version: 1
direction: direction/forward
top level: cI+luxR
start: 94
end: 97
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986779
display id: annotation1986779
version: 1
top level: cI+luxR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986779/range1986779/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986779/range1986779/1
type: Range
persistent identity: range1986779
display id: range1986779
version: 1
direction: direction/forward
top level: cI+luxR
start: 71
end: 76
orientation: inline

OR1 cI
type: SequenceAnnotation
persistent identity: annotation1986780
display id: annotation1986780
version: 1
top level: cI+luxR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986780/range1986780/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986780/range1986780/1
type: Range
persistent identity: range1986780
display id: range1986780
version: 1
direction: direction/forward
top level: cI+luxR
start: 81
end: 97
orientation: inline

BBa_R0065
type: SequenceAnnotation
persistent identity: annotation1986774
display id: annotation1986774
version: 1
top level: cI+luxR
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986774/range1986774/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986774/range1986774/1
type: Range
persistent identity: range1986774
display id: range1986774
version: 1
direction: direction/forward
top level: cI+luxR
start: 1
end: 97
orientation: inline

lux p(R) start
type: SequenceAnnotation
persistent identity: annotation1986778
display id: annotation1986778
version: 1
top level: cI+luxR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986778/range1986778/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986778/range1986778/1
type: Range
persistent identity: range1986778
display id: range1986778
version: 1
direction: direction/forward
top level: cI+luxR
start: 58
end: 58
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986776
display id: annotation1986776
version: 1
top level: cI+luxR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986776/range1986776/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986776/range1986776/1
type: Range
persistent identity: range1986776
display id: range1986776
version: 1
direction: direction/forward
top level: cI+luxR
start: 47
end: 52
orientation: inline

OR2 cI
type: SequenceAnnotation
persistent identity: annotation1986777
display id: annotation1986777
version: 1
top level: cI+luxR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986777/range1986777/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986777/range1986777/1
type: Range
persistent identity: range1986777
display id: range1986777
version: 1
direction: direction/forward
top level: cI+luxR
start: 57
end: 73
orientation: inline

BBa_K165043
type: ComponentDefinition
persistent identity: BBa K165043
display id: BBa_K165043
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Zif268-HIV binding sites + MET25 constitutive yeast promoter
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:56Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K165011
component: BBa_K165000
sequence annotation: BBa_K165011
sequence annotation: BBa_K165000
sequence: https://synbiohub.org/public/igem/BBa_K165043_sequence/1

BBa_K165011
type: Component
persistent identity: component1996569
display id: component1996569
version: 1
top level: BBa_K165043
access: public
definition: https://synbiohub.org/public/igem/BBa_K165011/1

BBa_K165000
type: Component
persistent identity: component1996570
display id: component1996570
version: 1
top level: BBa_K165043
access: public
definition: https://synbiohub.org/public/igem/BBa_K165000/1

BBa_K165011
type: SequenceAnnotation
persistent identity: annotation1996569
display id: annotation1996569
version: 1
top level: BBa_K165043
location: https://synbiohub.org/public/igem/BBa_K165043/annotation1996569/range1996569/1
component: BBa_K165011

https://synbiohub.org/public/igem/BBa_K165043/annotation1996569/range1996569/1
type: Range
persistent identity: range1996569
display id: range1996569
version: 1
direction: direction/reverse
top level: BBa_K165043
start: 1
end: 46
orientation: inline

BBa_K165000
type: SequenceAnnotation
persistent identity: annotation1996570
display id: annotation1996570
version: 1
top level: BBa_K165043
location: https://synbiohub.org/public/igem/BBa_K165043/annotation1996570/range1996570/1
component: BBa_K165000

https://synbiohub.org/public/igem/BBa_K165043/annotation1996570/range1996570/1
type: Range
persistent identity: range1996570
display id: range1996570
version: 1
direction: direction/reverse
top level: BBa_K165043
start: 55
end: 441
orientation: inline

las TetO
type: ComponentDefinition
persistent identity: BBa I14015
display id: BBa_I14015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: P(Las) TetO
creator: Vikram Vijayan, Allen Hsu, Lawrence Fomundam
created: 2004-07-29T11:00:00Z
modified: 2015-08-31T04:07:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _4_
m user id: 0
owner id: 171
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: LasR and 3OC12HSL regulated promoter with a single Tet Operator (binding site for TetR repressor)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: TetR
sequence annotation: -10
sequence annotation: R0079
sequence: https://synbiohub.org/public/igem/BBa_I14015_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation1026120
display id: annotation1026120
version: 1
top level: las TetO
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I14015/annotation1026120/range1026120/1

https://synbiohub.org/public/igem/BBa_I14015/annotation1026120/range1026120/1
type: Range
persistent identity: range1026120
display id: range1026120
version: 1
direction: direction/forward
top level: las TetO
start: 117
end: 122
orientation: inline

TetR
type: SequenceAnnotation
persistent identity: annotation1017910
display id: annotation1017910
version: 1
top level: las TetO
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I14015/annotation1017910/range1017910/1

https://synbiohub.org/public/igem/BBa_I14015/annotation1017910/range1017910/1
type: Range
persistent identity: range1017910
display id: range1017910
version: 1
direction: direction/forward
top level: las TetO
start: 152
end: 170
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1026116
display id: annotation1026116
version: 1
top level: las TetO
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I14015/annotation1026116/range1026116/1

https://synbiohub.org/public/igem/BBa_I14015/annotation1026116/range1026116/1
type: Range
persistent identity: range1026116
display id: range1026116
version: 1
direction: direction/forward
top level: las TetO
start: 140
end: 145
orientation: inline

R0079
type: SequenceAnnotation
persistent identity: annotation1017909
display id: annotation1017909
version: 1
top level: las TetO
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I14015/annotation1017909/range1017909/1

https://synbiohub.org/public/igem/BBa_I14015/annotation1017909/range1017909/1
type: Range
persistent identity: range1017909
display id: range1017909
version: 1
direction: direction/forward
top level: las TetO
start: 1
end: 151
orientation: inline

BBa_K112406
type: ComponentDefinition
persistent identity: BBa K112406
display id: BBa_K112406
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cadC promoter
creator: Bing Xia
created: 2008-10-21T11:00:00Z
modified: 2015-05-08T01:09:17Z
discontinued: false
dominant: false
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable provenance: Biobricking of cadC promoter and cadC gene PCR cadC-F and cadC-R on MG1655 (2336 bp, EcoR1/BamH1) Sub into pBca1256 (EcoR1/BamH1 910+2472bp, L) Product is pBca1256-K112406 ---------------------------- cb10013-cadC-F cgataGAATTCatgAGATCTcaccagcaatttgcgggcatatc cb10012-cad-R cgtagGGATCCtgaccagaccaaactggcg
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K112406_sequence/1

NAND01
type: ComponentDefinition
persistent identity: BBa I732303
display id: BBa_I732303
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NAND Candidate (U073O22D001O46)
creator: Ma Rui
created: 2007-10-02T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1592
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: U97NUL D001O11
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732303_sequence/1

BBa_I732451
type: ComponentDefinition
persistent identity: BBa I732451
display id: BBa_I732451
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U073O27+D062NUL)
creator: Zhan Jian
created: 2007-10-16T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732451_sequence/1

BBa_J64712
type: ComponentDefinition
persistent identity: BBa J64712
display id: BBa_J64712
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LasR/LasI Inducible & RHLR/RHLI repressible Promoter
creator: laura lavery
created: 2007-03-24T12:00:00Z
modified: 2015-05-08T01:08:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 1431
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is similar to BBa_R0079 but has an added binding site for RHLR/RHLI in between the -35 and -10 region. Thus this promoter is induced by LasR/LasI but repressed by RHLR/RHLI binding.
mutable notes: The natural spacer between the -35 and -10 is 14bp and the natural O1 site for RHLR/RHLI is 20bp long. There was not much over lap of the -35 or the -10 with this sequence, so it was nessicary to chop off some of the binding site sequence in order to maintain the correct spacing and preserve the -35 and -10 sequence. The binding site sequence is missing a tcc on is 5' end and a t on its 3' end.
mutable provenance: Rust L. et. al., "Analysis of the Pseudomonas aeruginosa Elastase (lasB) Regulatory Region". Journal of Bacteriology, 1996.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: -35
sequence annotation: RHLR/RHLI
sequence annotation: LasR/LasI O1
sequence annotation: LasR/LasI O2
sequence: https://synbiohub.org/public/igem/BBa_J64712_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation1921068
display id: annotation1921068
version: 1
top level: BBa_J64712
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J64712/annotation1921068/range1921068/1

https://synbiohub.org/public/igem/BBa_J64712/annotation1921068/range1921068/1
type: Range
persistent identity: range1921068
display id: range1921068
version: 1
direction: direction/forward
top level: BBa_J64712
start: 140
end: 145
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1921067
display id: annotation1921067
version: 1
top level: BBa_J64712
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J64712/annotation1921067/range1921067/1

https://synbiohub.org/public/igem/BBa_J64712/annotation1921067/range1921067/1
type: Range
persistent identity: range1921067
display id: range1921067
version: 1
direction: direction/forward
top level: BBa_J64712
start: 117
end: 122
orientation: inline

RHLR/RHLI
type: SequenceAnnotation
persistent identity: annotation1921066
display id: annotation1921066
version: 1
top level: BBa_J64712
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J64712/annotation1921066/range1921066/1

https://synbiohub.org/public/igem/BBa_J64712/annotation1921066/range1921066/1
type: Range
persistent identity: range1921066
display id: range1921066
version: 1
direction: direction/forward
top level: BBa_J64712
start: 125
end: 141
orientation: inline

LasR/LasI O1
type: SequenceAnnotation
persistent identity: annotation1921065
display id: annotation1921065
version: 1
top level: BBa_J64712
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_J64712/annotation1921065/range1921065/1

https://synbiohub.org/public/igem/BBa_J64712/annotation1921065/range1921065/1
type: Range
persistent identity: range1921065
display id: range1921065
version: 1
direction: direction/forward
top level: BBa_J64712
start: 106
end: 125
orientation: inline

LasR/LasI O2
type: SequenceAnnotation
persistent identity: annotation1921064
display id: annotation1921064
version: 1
top level: BBa_J64712
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_J64712/annotation1921064/range1921064/1

https://synbiohub.org/public/igem/BBa_J64712/annotation1921064/range1921064/1
type: Range
persistent identity: range1921064
display id: range1921064
version: 1
direction: direction/forward
top level: BBa_J64712
start: 46
end: 64
orientation: inline

BBa_J05209
type: ComponentDefinition
persistent identity: BBa J05209
display id: BBa_J05209
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Modifed Pr Promoter
creator: Alexander Roth
created: 2005-09-14T11:00:00Z
modified: 2015-08-31T04:08:15Z
discontinued: false
dominant: false
group u list: _34_
m user id: 0
owner id: 412
owning group id: 34
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is identical to BBa_R0051 exept that some base has been modifed to alow for additional ZFP repressors to bind
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: OR2
sequence annotation: -35
sequence annotation: ZFP binding sites
sequence: https://synbiohub.org/public/igem/BBa_J05209_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation1689812
display id: annotation1689812
version: 1
top level: BBa_J05209
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J05209/annotation1689812/range1689812/1

https://synbiohub.org/public/igem/BBa_J05209/annotation1689812/range1689812/1
type: Range
persistent identity: range1689812
display id: range1689812
version: 1
direction: direction/forward
top level: BBa_J05209
start: 38
end: 43
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation1689809
display id: annotation1689809
version: 1
top level: BBa_J05209
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J05209/annotation1689809/range1689809/1

https://synbiohub.org/public/igem/BBa_J05209/annotation1689809/range1689809/1
type: Range
persistent identity: range1689809
display id: range1689809
version: 1
direction: direction/forward
top level: BBa_J05209
start: 1
end: 17
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1689811
display id: annotation1689811
version: 1
top level: BBa_J05209
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J05209/annotation1689811/range1689811/1

https://synbiohub.org/public/igem/BBa_J05209/annotation1689811/range1689811/1
type: Range
persistent identity: range1689811
display id: range1689811
version: 1
direction: direction/forward
top level: BBa_J05209
start: 15
end: 20
orientation: inline

ZFP binding sites
type: SequenceAnnotation
persistent identity: annotation1689899
display id: annotation1689899
version: 1
top level: BBa_J05209
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J05209/annotation1689899/range1689899/1

https://synbiohub.org/public/igem/BBa_J05209/annotation1689899/range1689899/1
type: Range
persistent identity: range1689899
display id: range1689899
version: 1
direction: direction/forward
top level: BBa_J05209
start: 18
end: 29
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation1689810
display id: annotation1689810
version: 1
top level: BBa_J05209
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J05209/annotation1689810/range1689810/1

https://synbiohub.org/public/igem/BBa_J05209/annotation1689810/range1689810/1
type: Range
persistent identity: range1689810
display id: range1689810
version: 1
direction: direction/forward
top level: BBa_J05209
start: 25
end: 41
orientation: inline

BBa_K1486001
type: ComponentDefinition
persistent identity: BBa K1486001
display id: BBa_K1486001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Arabinose promoter + CpxR
creator: iGem EPFL 2014
created: 2014-08-11T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The part contains CpxR with an upstream arabinose-inducible promoter.
mutable notes: The arabinose had to be upstream of the CpxR. Also, the sequence had to be tested because of a mutation, and it works perfectly.
mutable provenance: The CpxR comes from the genome of E coli, and the arabinose promoter comes from a biobrick.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_B0034
component: BBa_I0500
component: BBa_K1486000
sequence annotation: BBa_K1486000
sequence annotation: BBa_I0500
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1486001_sequence/1

BBa_B0034
type: Component
persistent identity: component2382074
display id: component2382074
version: 1
top level: BBa_K1486001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I0500
type: Component
persistent identity: component2382072
display id: component2382072
version: 1
top level: BBa_K1486001
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_K1486000
type: Component
persistent identity: component2382078
display id: component2382078
version: 1
top level: BBa_K1486001
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2382078
display id: annotation2382078
version: 1
top level: BBa_K1486001
location: https://synbiohub.org/public/igem/BBa_K1486001/annotation2382078/range2382078/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486001/annotation2382078/range2382078/1
type: Range
persistent identity: range2382078
display id: range2382078
version: 1
direction: direction/reverse
top level: BBa_K1486001
start: 1223
end: 1924
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2382072
display id: annotation2382072
version: 1
top level: BBa_K1486001
location: https://synbiohub.org/public/igem/BBa_K1486001/annotation2382072/range2382072/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486001/annotation2382072/range2382072/1
type: Range
persistent identity: range2382072
display id: range2382072
version: 1
direction: direction/reverse
top level: BBa_K1486001
start: 1
end: 1210
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2382074
display id: annotation2382074
version: 1
top level: BBa_K1486001
location: https://synbiohub.org/public/igem/BBa_K1486001/annotation2382074/range2382074/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486001/annotation2382074/range2382074/1
type: Range
persistent identity: range2382074
display id: range2382074
version: 1
direction: direction/reverse
top level: BBa_K1486001
start: 1211
end: 1222
orientation: inline

BBa_K094120
type: ComponentDefinition
persistent identity: BBa K094120
display id: BBa_K094120
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLacI/ara-1
creator: Shi Lei
created: 2008-10-12T11:00:00Z
modified: 2015-05-08T01:08:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _255_
m user id: 0
owner id: 2589
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This promoter can be repressed by lacI protein and induced by either IPTG or arabinose.
mutable notes: IPTG has better effect on this promoter than arabinose
mutable provenance: Standard pLacI/ara-1 promoter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: pLac/ara-1
sequence: https://synbiohub.org/public/igem/BBa_K094120_sequence/1

pLac/ara-1
type: SequenceAnnotation
persistent identity: annotation1980631
display id: annotation1980631
version: 1
top level: BBa_K094120
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K094120/annotation1980631/range1980631/1

https://synbiohub.org/public/igem/BBa_K094120/annotation1980631/range1980631/1
type: Range
persistent identity: range1980631
display id: range1980631
version: 1
direction: direction/forward
top level: BBa_K094120
start: 1
end: 103
orientation: inline

BBa_K091104
type: ComponentDefinition
persistent identity: BBa K091104
display id: BBa_K091104
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLac/Mnt Hybrid Promoter
creator: Robert Cool
created: 2008-06-18T11:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _191_
m user id: 0
owner id: 3080
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This promoter is a modified version of the Mnt promoter that is also responsive to LacI. The promoter should be repressed by Mnt repressor. It should also be repressed by LacI, and in the absence of Mnt repressor, should be induced by IPTG.
mutable notes: Mnt repressor binds as a tetramer to two half-operator sites. Introduction of a LacI binding site between the Mnt promoter -10 sequence and the start site for transcription should allow for repression by LacI. Accordingly, the hybrid promoter was designed by 1) Remove all bases of mnt promoter 3??? to -10: gagtcgtattaattt will be replaced by tgtgtggaattgtga, and 2) Position lacI binding site (composed of an inverted repeat) from the lacI regulated promoter (R0010) immediately 3??? to truncated mnt promoter.
mutable provenance: This part comes from the Mnt promoter and the LacI promoter
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: LacI
sequence annotation: Mnt
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_K091104_sequence/1

LacI
type: SequenceAnnotation
persistent identity: annotation1963738
display id: annotation1963738
version: 1
top level: BBa_K091104
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K091104/annotation1963738/range1963738/1

https://synbiohub.org/public/igem/BBa_K091104/annotation1963738/range1963738/1
type: Range
persistent identity: range1963738
display id: range1963738
version: 1
direction: direction/forward
top level: BBa_K091104
start: 53
end: 87
orientation: inline

Mnt
type: SequenceAnnotation
persistent identity: annotation1963743
display id: annotation1963743
version: 1
top level: BBa_K091104
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K091104/annotation1963743/range1963743/1

https://synbiohub.org/public/igem/BBa_K091104/annotation1963743/range1963743/1
type: Range
persistent identity: range1963743
display id: range1963743
version: 1
direction: direction/forward
top level: BBa_K091104
start: 1
end: 52
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1963737
display id: annotation1963737
version: 1
top level: BBa_K091104
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K091104/annotation1963737/range1963737/1

https://synbiohub.org/public/igem/BBa_K091104/annotation1963737/range1963737/1
type: Range
persistent identity: range1963737
display id: range1963737
version: 1
direction: direction/forward
top level: BBa_K091104
start: 47
end: 52
orientation: inline

U073O11+D0
type: ComponentDefinition
persistent identity: BBa I732426
display id: BBa_I732426
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U073O11+D014O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732426_sequence/1

BBa_K101002
type: ComponentDefinition
persistent identity: BBa K101002
display id: BBa_K101002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Dual-Repressed Promoter for p22 cII and TetR
creator: Bennett Swiniarski
created: 2008-07-27T11:00:00Z
modified: 2015-05-08T01:08:41Z
discontinued: false
dominant: false
group u list: _205_
m user id: 0
owner id: 3363
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: k;kljg
mutable notes: TetR usually binds to two operator sites, one located upstream from -35 and one located between -35 and -10. However, p22 mnt also has two operator sites, one between -35 and -10 and another downstream from -10. Since each had an operator site in between -35 and -10, we had to eliminate the TetR operator site in order to allow the promoter to work. TetR was picked because it has a stronger binding than p22 mnt, and so would be more able to use only one site to bind.
mutable provenance: This part was designed by combining sequences from parts R0040 (TetR promoter) and R0053 (p22 cII promoter).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K101002_sequence/1

PN
type: ComponentDefinition
persistent identity: BBa K100000
display id: BBa_K100000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Natural Xylose Regulated Bi-Directional Operator
creator: Garrett Tobin
created: 2008-08-17T11:00:00Z
modified: 2015-05-08T01:08:41Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _213_
m user id: 0
owner id: 1752
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part contains the operator region controlling transcription for the xyl operon. This regulatory region is dependent upon the binding of CRP-cAMP and XylR bound to xylose for activation. Upon binding, transcription is activated in both directions (xylFGH and xylAB facing). This part has an unscrambled CRP binding site and and unaltered promoter region (unlike BBa_K100001 through BBa_K100003).
mutable notes: Both directions were kept in the sequence of the part even though it is intended to work in the right facing direction because of possible looping that may occur as a transcriptional control.
mutable provenance: Taken from E. Coli genome bases 3728830-3729092
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: RNA poly. binding site
sequence annotation: CRP binding site
sequence: https://synbiohub.org/public/igem/BBa_K100000_sequence/1

RNA poly. binding site
type: SequenceAnnotation
persistent identity: annotation1972196
display id: annotation1972196
version: 1
top level: PN
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K100000/annotation1972196/range1972196/1

https://synbiohub.org/public/igem/BBa_K100000/annotation1972196/range1972196/1
type: Range
persistent identity: range1972196
display id: range1972196
version: 1
direction: direction/forward
top level: PN
start: 45
end: 50
orientation: inline

RNA poly. binding site
type: SequenceAnnotation
persistent identity: annotation1972173
display id: annotation1972173
version: 1
top level: PN
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K100000/annotation1972173/range1972173/1

https://synbiohub.org/public/igem/BBa_K100000/annotation1972173/range1972173/1
type: Range
persistent identity: range1972173
display id: range1972173
version: 1
direction: direction/forward
top level: PN
start: 21
end: 26
orientation: inline

RNA poly. binding site
type: SequenceAnnotation
persistent identity: annotation1972263
display id: annotation1972263
version: 1
top level: PN
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K100000/annotation1972263/range1972263/1

https://synbiohub.org/public/igem/BBa_K100000/annotation1972263/range1972263/1
type: Range
persistent identity: range1972263
display id: range1972263
version: 1
direction: direction/forward
top level: PN
start: 243
end: 248
orientation: inline

CRP binding site
type: SequenceAnnotation
persistent identity: annotation1972261
display id: annotation1972261
version: 1
top level: PN
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K100000/annotation1972261/range1972261/1

https://synbiohub.org/public/igem/BBa_K100000/annotation1972261/range1972261/1
type: Range
persistent identity: range1972261
display id: range1972261
version: 1
direction: direction/forward
top level: PN
start: 90
end: 107
orientation: inline

RNA poly. binding site
type: SequenceAnnotation
persistent identity: annotation1972266
display id: annotation1972266
version: 1
top level: PN
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K100000/annotation1972266/range1972266/1

https://synbiohub.org/public/igem/BBa_K100000/annotation1972266/range1972266/1
type: Range
persistent identity: range1972266
display id: range1972266
version: 1
direction: direction/forward
top level: PN
start: 264
end: 269
orientation: inline

U061O11+D0
type: ComponentDefinition
persistent identity: BBa I732434
display id: BBa_I732434
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U061O11+D038O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732434_sequence/1

BBa_K101001
type: ComponentDefinition
persistent identity: BBa K101001
display id: BBa_K101001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Dual-Repressed Promoter for LacI and LambdacI
creator: Bennett Swiniarski
created: 2008-07-27T11:00:00Z
modified: 2015-05-08T01:08:41Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _205_
m user id: 0
owner id: 3363
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is a new construct of a promoter that is dually repressed by either LacI or LambdacI proteins.
mutable notes: LambdacI usually binds to two operator sites, one located upstream from -35 and one located between -35 and -10. By itself, LacI binds downstream from -10. This made the part design very straightforward, since the locations of the operator sites do not overlap at all. In order to complete this part, we simply combined the proper sequences and we had a dually-repressed promoter.
mutable provenance: This part was made by combining BioBrick parts R0010(LacI promoter) and R0051 (LambdacI promoter), along with intermediate sequence between these operator sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K101001_sequence/1

BBa_K1486040
type: ComponentDefinition
persistent identity: BBa K1486040
display id: BBa_K1486040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sfGFPN + CpxR
creator: EPFL iGem team 2014
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1486038
component: BBa_B0034
component: BBa_K1486000
component: BBa_I0500
component: BBa_K1486037
sequence annotation: BBa_K1486038
sequence annotation: BBa_K1486037
sequence annotation: BBa_I0500
sequence annotation: BBa_K1486000
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1486040_sequence/1

BBa_K1486038
type: Component
persistent identity: component2387188
display id: component2387188
version: 1
top level: BBa_K1486040
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486038/1

BBa_B0034
type: Component
persistent identity: component2387187
display id: component2387187
version: 1
top level: BBa_K1486040
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486000
type: Component
persistent identity: component2387193
display id: component2387193
version: 1
top level: BBa_K1486040
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_I0500
type: Component
persistent identity: component2387185
display id: component2387185
version: 1
top level: BBa_K1486040
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_K1486037
type: Component
persistent identity: component2387189
display id: component2387189
version: 1
top level: BBa_K1486040
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486037/1

BBa_K1486038
type: SequenceAnnotation
persistent identity: annotation2387188
display id: annotation2387188
version: 1
top level: BBa_K1486040
location: https://synbiohub.org/public/igem/BBa_K1486040/annotation2387188/range2387188/1
component: BBa_K1486038

https://synbiohub.org/public/igem/BBa_K1486040/annotation2387188/range2387188/1
type: Range
persistent identity: range2387188
display id: range2387188
version: 1
direction: direction/reverse
top level: BBa_K1486040
start: 1223
end: 1864
orientation: inline

BBa_K1486037
type: SequenceAnnotation
persistent identity: annotation2387189
display id: annotation2387189
version: 1
top level: BBa_K1486040
location: https://synbiohub.org/public/igem/BBa_K1486040/annotation2387189/range2387189/1
component: BBa_K1486037

https://synbiohub.org/public/igem/BBa_K1486040/annotation2387189/range2387189/1
type: Range
persistent identity: range2387189
display id: range2387189
version: 1
direction: direction/reverse
top level: BBa_K1486040
start: 1865
end: 1903
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2387185
display id: annotation2387185
version: 1
top level: BBa_K1486040
location: https://synbiohub.org/public/igem/BBa_K1486040/annotation2387185/range2387185/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486040/annotation2387185/range2387185/1
type: Range
persistent identity: range2387185
display id: range2387185
version: 1
direction: direction/reverse
top level: BBa_K1486040
start: 1
end: 1210
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2387193
display id: annotation2387193
version: 1
top level: BBa_K1486040
location: https://synbiohub.org/public/igem/BBa_K1486040/annotation2387193/range2387193/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486040/annotation2387193/range2387193/1
type: Range
persistent identity: range2387193
display id: range2387193
version: 1
direction: direction/reverse
top level: BBa_K1486040
start: 1904
end: 2605
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2387187
display id: annotation2387187
version: 1
top level: BBa_K1486040
location: https://synbiohub.org/public/igem/BBa_K1486040/annotation2387187/range2387187/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486040/annotation2387187/range2387187/1
type: Range
persistent identity: range2387187
display id: range2387187
version: 1
direction: direction/reverse
top level: BBa_K1486040
start: 1211
end: 1222
orientation: inline

U049O11+D0
type: ComponentDefinition
persistent identity: BBa I732442
display id: BBa_I732442
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U049O11+D062O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732442_sequence/1

BBa_K266007
type: ComponentDefinition
persistent identity: BBa K266007
display id: BBa_K266007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Complex QS -> LuxI & LasI circuit
creator: UNAM FCIENCIAS-CINVESTAV IRA
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _363_
m user id: 0
owner id: 1955
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Complex Quorum sensing circuit that receives the signal of PAI+LasR and AI+LuxR to control the production of LuxI and LasI enzymes.
mutable notes: None
mutable provenance: The biobricks BBa_K266006 and BBa_K266000 where used to build this biobrick
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_K266000
component: BBa_K266006
sequence annotation: BBa_K266006
sequence annotation: BBa_K266000
sequence: https://synbiohub.org/public/igem/BBa_K266007_sequence/1

BBa_K266000
type: Component
persistent identity: component2297452
display id: component2297452
version: 1
top level: BBa_K266007
access: public
definition: https://synbiohub.org/public/igem/BBa_K266000/1

BBa_K266006
type: Component
persistent identity: component2297433
display id: component2297433
version: 1
top level: BBa_K266007
access: public
definition: BBa_K266006

BBa_K266006
type: SequenceAnnotation
persistent identity: annotation2297433
display id: annotation2297433
version: 1
top level: BBa_K266007
location: https://synbiohub.org/public/igem/BBa_K266007/annotation2297433/range2297433/1
component: BBa_K266006

https://synbiohub.org/public/igem/BBa_K266007/annotation2297433/range2297433/1
type: Range
persistent identity: range2297433
display id: range2297433
version: 1
direction: direction/reverse
top level: BBa_K266007
start: 1
end: 1705
orientation: inline

BBa_K266000
type: SequenceAnnotation
persistent identity: annotation2297452
display id: annotation2297452
version: 1
top level: BBa_K266007
location: https://synbiohub.org/public/igem/BBa_K266007/annotation2297452/range2297452/1
component: BBa_K266000

https://synbiohub.org/public/igem/BBa_K266007/annotation2297452/range2297452/1
type: Range
persistent identity: range2297452
display id: range2297452
version: 1
direction: direction/reverse
top level: BBa_K266007
start: 1714
end: 2676
orientation: inline

BBa_K1486002
type: ComponentDefinition
persistent identity: BBa K1486002
display id: BBa_K1486002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Arabinose promoter + sfGFP CpxR (Nterm)
creator: iGem EPFL 2014
created: 2014-08-11T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is composed of an arabinose-inducible promoter for superfolded GFP attached by a linker to CpxR (on the N terminus of CpxR).
mutable notes: We had to test both attachments, N and C terminus of the CpxR.
mutable provenance: The arabinose promoter and the sfGFP were obtained from the registry, while the CpxR was obtained from the E coli genome.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1486000
component: BBa_K1486003
component: BBa_I746916
component: BBa_B0034
component: BBa_I0500
sequence annotation: BBa_I746916
sequence annotation: BBa_K1486003
sequence annotation: BBa_I0500
sequence annotation: BBa_B0034
sequence annotation: BBa_K1486000
sequence: https://synbiohub.org/public/igem/BBa_K1486002_sequence/1

BBa_K1486000
type: Component
persistent identity: component2382068
display id: component2382068
version: 1
top level: BBa_K1486002
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_K1486003
type: Component
persistent identity: component2382064
display id: component2382064
version: 1
top level: BBa_K1486002
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486003/1

BBa_I746916
type: Component
persistent identity: component2382063
display id: component2382063
version: 1
top level: BBa_K1486002
access: public
definition: https://synbiohub.org/public/igem/BBa_I746916/1

BBa_B0034
type: Component
persistent identity: component2382059
display id: component2382059
version: 1
top level: BBa_K1486002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I0500
type: Component
persistent identity: component2382057
display id: component2382057
version: 1
top level: BBa_K1486002
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_I746916
type: SequenceAnnotation
persistent identity: annotation2382063
display id: annotation2382063
version: 1
top level: BBa_K1486002
location: https://synbiohub.org/public/igem/BBa_K1486002/annotation2382063/range2382063/1
component: BBa_I746916

https://synbiohub.org/public/igem/BBa_K1486002/annotation2382063/range2382063/1
type: Range
persistent identity: range2382063
display id: range2382063
version: 1
direction: direction/reverse
top level: BBa_K1486002
start: 1223
end: 1942
orientation: inline

BBa_K1486003
type: SequenceAnnotation
persistent identity: annotation2382064
display id: annotation2382064
version: 1
top level: BBa_K1486002
location: https://synbiohub.org/public/igem/BBa_K1486002/annotation2382064/range2382064/1
component: BBa_K1486003

https://synbiohub.org/public/igem/BBa_K1486002/annotation2382064/range2382064/1
type: Range
persistent identity: range2382064
display id: range2382064
version: 1
direction: direction/reverse
top level: BBa_K1486002
start: 1943
end: 1966
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2382057
display id: annotation2382057
version: 1
top level: BBa_K1486002
location: https://synbiohub.org/public/igem/BBa_K1486002/annotation2382057/range2382057/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486002/annotation2382057/range2382057/1
type: Range
persistent identity: range2382057
display id: range2382057
version: 1
direction: direction/reverse
top level: BBa_K1486002
start: 1
end: 1210
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2382059
display id: annotation2382059
version: 1
top level: BBa_K1486002
location: https://synbiohub.org/public/igem/BBa_K1486002/annotation2382059/range2382059/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486002/annotation2382059/range2382059/1
type: Range
persistent identity: range2382059
display id: range2382059
version: 1
direction: direction/reverse
top level: BBa_K1486002
start: 1211
end: 1222
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2382068
display id: annotation2382068
version: 1
top level: BBa_K1486002
location: https://synbiohub.org/public/igem/BBa_K1486002/annotation2382068/range2382068/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486002/annotation2382068/range2382068/1
type: Range
persistent identity: range2382068
display id: range2382068
version: 1
direction: direction/reverse
top level: BBa_K1486002
start: 1967
end: 2668
orientation: inline

BBa_I714924
type: ComponentDefinition
persistent identity: BBa I714924
display id: BBa_I714924
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RecA_DlexO_DLacO1
creator: Chunbo Lou
created: 2007-10-21T11:00:00Z
modified: 2015-08-31T04:07:48Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _142_
m user id: 0
owner id: 1911
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: a NOR gate
mutable notes: NOR gat
mutable provenance: synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: LacO1
sequence annotation: -10
sequence annotation: SDY_EGFP
sequence annotation: LexA
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_I714924_sequence/1

LacO1
type: SequenceAnnotation
persistent identity: annotation1951924
display id: annotation1951924
version: 1
top level: BBa_I714924
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I714924/annotation1951924/range1951924/1

https://synbiohub.org/public/igem/BBa_I714924/annotation1951924/range1951924/1
type: Range
persistent identity: range1951924
display id: range1951924
version: 1
direction: direction/forward
top level: BBa_I714924
start: 93
end: 112
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1951926
display id: annotation1951926
version: 1
top level: BBa_I714924
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I714924/annotation1951926/range1951926/1

https://synbiohub.org/public/igem/BBa_I714924/annotation1951926/range1951926/1
type: Range
persistent identity: range1951926
display id: range1951926
version: 1
direction: direction/forward
top level: BBa_I714924
start: 81
end: 86
orientation: inline

LacO1
type: SequenceAnnotation
persistent identity: annotation1951921
display id: annotation1951921
version: 1
top level: BBa_I714924
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I714924/annotation1951921/range1951921/1

https://synbiohub.org/public/igem/BBa_I714924/annotation1951921/range1951921/1
type: Range
persistent identity: range1951921
display id: range1951921
version: 1
direction: direction/forward
top level: BBa_I714924
start: 1
end: 21
orientation: inline

SDY_EGFP
type: SequenceAnnotation
persistent identity: annotation1956422
display id: annotation1956422
version: 1
top level: BBa_I714924
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_I714924/annotation1956422/range1956422/1

https://synbiohub.org/public/igem/BBa_I714924/annotation1956422/range1956422/1
type: Range
persistent identity: range1956422
display id: range1956422
version: 1
direction: direction/forward
top level: BBa_I714924
start: 121
end: 862
orientation: inline

LexA
type: SequenceAnnotation
persistent identity: annotation1951922
display id: annotation1951922
version: 1
top level: BBa_I714924
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I714924/annotation1951922/range1951922/1

https://synbiohub.org/public/igem/BBa_I714924/annotation1951922/range1951922/1
type: Range
persistent identity: range1951922
display id: range1951922
version: 1
direction: direction/forward
top level: BBa_I714924
start: 65
end: 80
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1951925
display id: annotation1951925
version: 1
top level: BBa_I714924
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I714924/annotation1951925/range1951925/1

https://synbiohub.org/public/igem/BBa_I714924/annotation1951925/range1951925/1
type: Range
persistent identity: range1951925
display id: range1951925
version: 1
direction: direction/forward
top level: BBa_I714924
start: 59
end: 64
orientation: inline

LexA
type: SequenceAnnotation
persistent identity: annotation1956155
display id: annotation1956155
version: 1
top level: BBa_I714924
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I714924/annotation1956155/range1956155/1

https://synbiohub.org/public/igem/BBa_I714924/annotation1956155/range1956155/1
type: Range
persistent identity: range1956155
display id: range1956155
version: 1
direction: direction/forward
top level: BBa_I714924
start: 35
end: 55
orientation: inline

U037O11+D0
type: ComponentDefinition
persistent identity: BBa I732406
display id: BBa_I732406
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U037O11+D062NUL)
creator: Zhan Jian
created: 2007-09-19T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1572
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: A Double Reporter (LacZ-alpha and GFP-AAV) loaded on pSB3K5. For more information please refer to BBa_I732403.
mutable notes: Might be later edited.
mutable provenance: Might be later edited.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: conserved
sequence annotation: BBa_B0012
sequence annotation: BamHI
sequence annotation: stop
sequence annotation: BBa_I732006
sequence annotation: BBa_B0034
sequence annotation: BBa_E0040
sequence annotation: BglII
sequence annotation: GFP protein
sequence annotation: BBa_I732007
sequence annotation: T7 TE
sequence annotation: START
sequence annotation: STOP
sequence annotation: stem_loop
sequence annotation: polya
sequence annotation: BBa_B0010
sequence annotation: lacZ
sequence: https://synbiohub.org/public/igem/BBa_I732406_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation1944463
display id: annotation1944463
version: 1
top level: U037O11+D0
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944463/range1944463/1

https://synbiohub.org/public/igem/BBa_I732406/annotation1944463/range1944463/1
type: Range
persistent identity: range1944463
display id: range1944463
version: 1
direction: direction/forward
top level: U037O11+D0
start: 32
end: 35
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1944475
display id: annotation1944475
version: 1
top level: U037O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944475/range1944475/1

https://synbiohub.org/public/igem/BBa_I732406/annotation1944475/range1944475/1
type: Range
persistent identity: range1944475
display id: range1944475
version: 1
direction: direction/forward
top level: U037O11+D0
start: 1122
end: 1162
orientation: inline

BamHI
type: SequenceAnnotation
persistent identity: annotation1944461
display id: annotation1944461
version: 1
top level: U037O11+D0
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944461/range1944461/1

https://synbiohub.org/public/igem/BBa_I732406/annotation1944461/range1944461/1
type: Range
persistent identity: range1944461
display id: range1944461
version: 1
direction: direction/forward
top level: U037O11+D0
start: 12
end: 17
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1944478
display id: annotation1944478
version: 1
top level: U037O11+D0
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944478/range1944478/1

https://synbiohub.org/public/igem/BBa_I732406/annotation1944478/range1944478/1
type: Range
persistent identity: range1944478
display id: range1944478
version: 1
direction: direction/forward
top level: U037O11+D0
start: 1155
end: 1155
orientation: inline

BBa_I732006
type: SequenceAnnotation
persistent identity: annotation1944468
display id: annotation1944468
version: 1
top level: U037O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944468/range1944468/1

https://synbiohub.org/public/igem/BBa_I732406/annotation1944468/range1944468/1
type: Range
persistent identity: range1944468
display id: range1944468
version: 1
direction: direction/reverse
top level: U037O11+D0
start: 46
end: 279
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation1944469
display id: annotation1944469
version: 1
top level: U037O11+D0
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944469/range1944469/1

https://synbiohub.org/public/igem/BBa_I732406/annotation1944469/range1944469/1
type: Range
persistent identity: range1944469
display id: range1944469
version: 1
direction: direction/forward
top level: U037O11+D0
start: 292
end: 295
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1944470
display id: annotation1944470
version: 1
top level: U037O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944470/range1944470/1

https://synbiohub.org/public/igem/BBa_I732406/annotation1944470/range1944470/1
type: Range
persistent identity: range1944470
display id: range1944470
version: 1
direction: direction/reverse
top level: U037O11+D0
start: 288
end: 299
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1944472
display id: annotation1944472
version: 1
top level: U037O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944472/range1944472/1

https://synbiohub.org/public/igem/BBa_I732406/annotation1944472/range1944472/1
type: Range
persistent identity: range1944472
display id: range1944472
version: 1
direction: direction/reverse
top level: U037O11+D0
start: 306
end: 1025
orientation: inline

BglII
type: SequenceAnnotation
persistent identity: annotation1944460
display id: annotation1944460
version: 1
top level: U037O11+D0
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944460/range1944460/1

https://synbiohub.org/public/igem/BBa_I732406/annotation1944460/range1944460/1
type: Range
persistent identity: range1944460
display id: range1944460
version: 1
direction: direction/forward
top level: U037O11+D0
start: 3
end: 8
orientation: inline

GFP protein
type: SequenceAnnotation
persistent identity: annotation1944471
display id: annotation1944471
version: 1
top level: U037O11+D0
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944471/range1944471/1

https://synbiohub.org/public/igem/BBa_I732406/annotation1944471/range1944471/1
type: Range
persistent identity: range1944471
display id: range1944471
version: 1
direction: direction/forward
top level: U037O11+D0
start: 306
end: 1025
orientation: inline

BBa_I732007
type: SequenceAnnotation
persistent identity: annotation1944462
display id: annotation1944462
version: 1
top level: U037O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944462/range1944462/1

https://synbiohub.org/public/igem/BBa_I732406/annotation1944462/range1944462/1
type: Range
persistent identity: range1944462
display id: range1944462
version: 1
direction: direction/reverse
top level: U037O11+D0
start: 1
end: 19
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1944476
display id: annotation1944476
version: 1
top level: U037O11+D0
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944476/range1944476/1

https://synbiohub.org/public/igem/BBa_I732406/annotation1944476/range1944476/1
type: Range
persistent identity: range1944476
display id: range1944476
version: 1
direction: direction/forward
top level: U037O11+D0
start: 1129
end: 1148
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1944464
display id: annotation1944464
version: 1
top level: U037O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944464/range1944464/1

https://synbiohub.org/public/igem/BBa_I732406/annotation1944464/range1944464/1
type: Range
persistent identity: range1944464
display id: range1944464
version: 1
direction: direction/reverse
top level: U037O11+D0
start: 28
end: 39
orientation: inline

START
type: SequenceAnnotation
persistent identity: annotation1944465
display id: annotation1944465
version: 1
top level: U037O11+D0
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944465/range1944465/1

https://synbiohub.org/public/igem/BBa_I732406/annotation1944465/range1944465/1
type: Range
persistent identity: range1944465
display id: range1944465
version: 1
direction: direction/forward
top level: U037O11+D0
start: 46
end: 48
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation1944467
display id: annotation1944467
version: 1
top level: U037O11+D0
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944467/range1944467/1

https://synbiohub.org/public/igem/BBa_I732406/annotation1944467/range1944467/1
type: Range
persistent identity: range1944467
display id: range1944467
version: 1
direction: direction/forward
top level: U037O11+D0
start: 274
end: 279
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1944474
display id: annotation1944474
version: 1
top level: U037O11+D0
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944474/range1944474/1

https://synbiohub.org/public/igem/BBa_I732406/annotation1944474/range1944474/1
type: Range
persistent identity: range1944474
display id: range1944474
version: 1
direction: direction/forward
top level: U037O11+D0
start: 1045
end: 1088
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1944477
display id: annotation1944477
version: 1
top level: U037O11+D0
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944477/range1944477/1

https://synbiohub.org/public/igem/BBa_I732406/annotation1944477/range1944477/1
type: Range
persistent identity: range1944477
display id: range1944477
version: 1
direction: direction/forward
top level: U037O11+D0
start: 1149
end: 1162
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1944473
display id: annotation1944473
version: 1
top level: U037O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944473/range1944473/1

https://synbiohub.org/public/igem/BBa_I732406/annotation1944473/range1944473/1
type: Range
persistent identity: range1944473
display id: range1944473
version: 1
direction: direction/forward
top level: U037O11+D0
start: 1034
end: 1113
orientation: inline

lacZ
type: SequenceAnnotation
persistent identity: annotation1944466
display id: annotation1944466
version: 1
top level: U037O11+D0
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944466/range1944466/1

https://synbiohub.org/public/igem/BBa_I732406/annotation1944466/range1944466/1
type: Range
persistent identity: range1944466
display id: range1944466
version: 1
direction: direction/forward
top level: U037O11+D0
start: 46
end: 279
orientation: inline

U037O11+D0
type: ComponentDefinition
persistent identity: BBa I732448
display id: BBa_I732448
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U037O11+D062O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732448_sequence/1

U097NUL+D0
type: ComponentDefinition
persistent identity: BBa I732407
display id: BBa_I732407
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U097NUL+D002O22)
creator: Zhan Jian
created: 2007-09-19T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1572
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: A Double Reporter (LacZ-alpha and GFP-AAV) loaded on pSB3C5. For more information please refer to BBa_I732401.
mutable notes: Might be later edited.
mutable provenance: Might be later edited.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_B0034
sequence annotation: stem_loop
sequence annotation: BamHI
sequence annotation: START
sequence annotation: lacZ
sequence annotation: polya
sequence annotation: T7 TE
sequence annotation: BBa_E0044
sequence annotation: BBa_B0012
sequence annotation: stop
sequence annotation: BglII
sequence annotation: conserved
sequence annotation: BBa_I732007
sequence annotation: BBa_B0010
sequence annotation: STOP
sequence annotation: BBa_I732006
sequence: https://synbiohub.org/public/igem/BBa_I732407_sequence/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1944483
display id: annotation1944483
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944483/range1944483/1

https://synbiohub.org/public/igem/BBa_I732407/annotation1944483/range1944483/1
type: Range
persistent identity: range1944483
display id: range1944483
version: 1
direction: direction/reverse
top level: U097NUL+D0
start: 28
end: 39
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1944492
display id: annotation1944492
version: 1
top level: U097NUL+D0
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944492/range1944492/1

https://synbiohub.org/public/igem/BBa_I732407/annotation1944492/range1944492/1
type: Range
persistent identity: range1944492
display id: range1944492
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 1084
end: 1127
orientation: inline

BamHI
type: SequenceAnnotation
persistent identity: annotation1944480
display id: annotation1944480
version: 1
top level: U097NUL+D0
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944480/range1944480/1

https://synbiohub.org/public/igem/BBa_I732407/annotation1944480/range1944480/1
type: Range
persistent identity: range1944480
display id: range1944480
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 12
end: 17
orientation: inline

START
type: SequenceAnnotation
persistent identity: annotation1944484
display id: annotation1944484
version: 1
top level: U097NUL+D0
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944484/range1944484/1

https://synbiohub.org/public/igem/BBa_I732407/annotation1944484/range1944484/1
type: Range
persistent identity: range1944484
display id: range1944484
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 46
end: 48
orientation: inline

lacZ
type: SequenceAnnotation
persistent identity: annotation1944485
display id: annotation1944485
version: 1
top level: U097NUL+D0
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944485/range1944485/1

https://synbiohub.org/public/igem/BBa_I732407/annotation1944485/range1944485/1
type: Range
persistent identity: range1944485
display id: range1944485
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 46
end: 279
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1944495
display id: annotation1944495
version: 1
top level: U097NUL+D0
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944495/range1944495/1

https://synbiohub.org/public/igem/BBa_I732407/annotation1944495/range1944495/1
type: Range
persistent identity: range1944495
display id: range1944495
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 1188
end: 1201
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1944489
display id: annotation1944489
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944489/range1944489/1

https://synbiohub.org/public/igem/BBa_I732407/annotation1944489/range1944489/1
type: Range
persistent identity: range1944489
display id: range1944489
version: 1
direction: direction/reverse
top level: U097NUL+D0
start: 288
end: 299
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1944494
display id: annotation1944494
version: 1
top level: U097NUL+D0
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944494/range1944494/1

https://synbiohub.org/public/igem/BBa_I732407/annotation1944494/range1944494/1
type: Range
persistent identity: range1944494
display id: range1944494
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 1168
end: 1187
orientation: inline

BBa_E0044
type: SequenceAnnotation
persistent identity: annotation1944490
display id: annotation1944490
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944490/range1944490/1

https://synbiohub.org/public/igem/BBa_I732407/annotation1944490/range1944490/1
type: Range
persistent identity: range1944490
display id: range1944490
version: 1
direction: direction/reverse
top level: U097NUL+D0
start: 306
end: 1064
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1944493
display id: annotation1944493
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944493/range1944493/1

https://synbiohub.org/public/igem/BBa_I732407/annotation1944493/range1944493/1
type: Range
persistent identity: range1944493
display id: range1944493
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 1161
end: 1201
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1944496
display id: annotation1944496
version: 1
top level: U097NUL+D0
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944496/range1944496/1

https://synbiohub.org/public/igem/BBa_I732407/annotation1944496/range1944496/1
type: Range
persistent identity: range1944496
display id: range1944496
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 1194
end: 1194
orientation: inline

BglII
type: SequenceAnnotation
persistent identity: annotation1944479
display id: annotation1944479
version: 1
top level: U097NUL+D0
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944479/range1944479/1

https://synbiohub.org/public/igem/BBa_I732407/annotation1944479/range1944479/1
type: Range
persistent identity: range1944479
display id: range1944479
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 3
end: 8
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation1944488
display id: annotation1944488
version: 1
top level: U097NUL+D0
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944488/range1944488/1

https://synbiohub.org/public/igem/BBa_I732407/annotation1944488/range1944488/1
type: Range
persistent identity: range1944488
display id: range1944488
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 292
end: 295
orientation: inline

BBa_I732007
type: SequenceAnnotation
persistent identity: annotation1944481
display id: annotation1944481
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944481/range1944481/1

https://synbiohub.org/public/igem/BBa_I732407/annotation1944481/range1944481/1
type: Range
persistent identity: range1944481
display id: range1944481
version: 1
direction: direction/reverse
top level: U097NUL+D0
start: 1
end: 19
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1944491
display id: annotation1944491
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944491/range1944491/1

https://synbiohub.org/public/igem/BBa_I732407/annotation1944491/range1944491/1
type: Range
persistent identity: range1944491
display id: range1944491
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 1073
end: 1152
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation1944482
display id: annotation1944482
version: 1
top level: U097NUL+D0
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944482/range1944482/1

https://synbiohub.org/public/igem/BBa_I732407/annotation1944482/range1944482/1
type: Range
persistent identity: range1944482
display id: range1944482
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 32
end: 35
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation1944486
display id: annotation1944486
version: 1
top level: U097NUL+D0
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944486/range1944486/1

https://synbiohub.org/public/igem/BBa_I732407/annotation1944486/range1944486/1
type: Range
persistent identity: range1944486
display id: range1944486
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 274
end: 279
orientation: inline

BBa_I732006
type: SequenceAnnotation
persistent identity: annotation1944487
display id: annotation1944487
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944487/range1944487/1

https://synbiohub.org/public/igem/BBa_I732407/annotation1944487/range1944487/1
type: Range
persistent identity: range1944487
display id: range1944487
version: 1
direction: direction/reverse
top level: U097NUL+D0
start: 46
end: 279
orientation: inline

U097O11+D0
type: ComponentDefinition
persistent identity: BBa I732413
display id: BBa_I732413
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U097O11+D002O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732413_sequence/1

BBa_K091143
type: ComponentDefinition
persistent identity: BBa K091143
display id: BBa_K091143
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLas/cI Hybrid Promoter
creator: Xiao Zhu
created: 2008-07-14T11:00:00Z
modified: 2015-05-08T01:08:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _191_
m user id: 0
owner id: 3130
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: this hybrid promoter is designed to be consecutively off.It combines with plasR(R0079) and lambda cI OR1(see C0051).Adding P-AI-1 sould activate the promoter, while repressed by lambda cI.
mutable notes: "CI binding of the OR region. It binds OR1 with highest affinity, OR2 with next highest, and OR3 with lowest"(http://www.nonanone.com/qw/files/Biology%204450/test2_lecture8,9.txt)
mutable provenance: .
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: plasR OP1
sequence annotation: plasR OP2
sequence annotation: -35
sequence annotation: cI OR1
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_K091143_sequence/1

plasR OP1
type: SequenceAnnotation
persistent identity: annotation1967107
display id: annotation1967107
version: 1
top level: BBa_K091143
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K091143/annotation1967107/range1967107/1

https://synbiohub.org/public/igem/BBa_K091143/annotation1967107/range1967107/1
type: Range
persistent identity: range1967107
display id: range1967107
version: 1
direction: direction/forward
top level: BBa_K091143
start: 46
end: 63
orientation: inline

plasR OP2
type: SequenceAnnotation
persistent identity: annotation1967108
display id: annotation1967108
version: 1
top level: BBa_K091143
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K091143/annotation1967108/range1967108/1

https://synbiohub.org/public/igem/BBa_K091143/annotation1967108/range1967108/1
type: Range
persistent identity: range1967108
display id: range1967108
version: 1
direction: direction/forward
top level: BBa_K091143
start: 106
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1967105
display id: annotation1967105
version: 1
top level: BBa_K091143
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K091143/annotation1967105/range1967105/1

https://synbiohub.org/public/igem/BBa_K091143/annotation1967105/range1967105/1
type: Range
persistent identity: range1967105
display id: range1967105
version: 1
direction: direction/forward
top level: BBa_K091143
start: 117
end: 122
orientation: inline

cI OR1
type: SequenceAnnotation
persistent identity: annotation1967106
display id: annotation1967106
version: 1
top level: BBa_K091143
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K091143/annotation1967106/range1967106/1

https://synbiohub.org/public/igem/BBa_K091143/annotation1967106/range1967106/1
type: Range
persistent identity: range1967106
display id: range1967106
version: 1
direction: direction/forward
top level: BBa_K091143
start: 147
end: 164
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1967104
display id: annotation1967104
version: 1
top level: BBa_K091143
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K091143/annotation1967104/range1967104/1

https://synbiohub.org/public/igem/BBa_K091143/annotation1967104/range1967104/1
type: Range
persistent identity: range1967104
display id: range1967104
version: 1
direction: direction/forward
top level: BBa_K091143
start: 140
end: 145
orientation: inline

BBa_I739103
type: ComponentDefinition
persistent identity: BBa I739103
display id: BBa_I739103
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Double Promoter (lacI, negative / P22 cII, negative)
creator: Stefan Luzi
created: 2007-10-14T11:00:00Z
modified: 2015-08-31T04:08:01Z
discontinued: false
dominant: false
group u list: _124_
m user id: 0
owner id: 2183
owning group id: 62
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: asdf
mutable notes: asdf
mutable provenance: asf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OR1
sequence annotation: R0053 (2x OR1)
sequence annotation: R0011
sequence: https://synbiohub.org/public/igem/BBa_I739103_sequence/1

OR1
type: SequenceAnnotation
persistent identity: annotation1947821
display id: annotation1947821
version: 1
top level: BBa_I739103
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I739103/annotation1947821/range1947821/1

https://synbiohub.org/public/igem/BBa_I739103/annotation1947821/range1947821/1
type: Range
persistent identity: range1947821
display id: range1947821
version: 1
direction: direction/forward
top level: BBa_I739103
start: 50
end: 68
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation1947822
display id: annotation1947822
version: 1
top level: BBa_I739103
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I739103/annotation1947822/range1947822/1

https://synbiohub.org/public/igem/BBa_I739103/annotation1947822/range1947822/1
type: Range
persistent identity: range1947822
display id: range1947822
version: 1
direction: direction/forward
top level: BBa_I739103
start: 69
end: 87
orientation: inline

R0053 (2x OR1)
type: SequenceAnnotation
persistent identity: annotation1947823
display id: annotation1947823
version: 1
top level: BBa_I739103
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I739103/annotation1947823/range1947823/1

https://synbiohub.org/public/igem/BBa_I739103/annotation1947823/range1947823/1
type: Range
persistent identity: range1947823
display id: range1947823
version: 1
direction: direction/forward
top level: BBa_I739103
start: 50
end: 87
orientation: inline

R0011
type: SequenceAnnotation
persistent identity: annotation1947820
display id: annotation1947820
version: 1
top level: BBa_I739103
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I739103/annotation1947820/range1947820/1

https://synbiohub.org/public/igem/BBa_I739103/annotation1947820/range1947820/1
type: Range
persistent identity: range1947820
display id: range1947820
version: 1
direction: direction/forward
top level: BBa_I739103
start: 1
end: 48
orientation: inline

pAND
type: ComponentDefinition
persistent identity: BBa J58100
display id: BBa_J58100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AND-type promoter synergistically activated by cI and CRP
creator: Valencia iGEM 2006
created: 2006-10-25T11:00:00Z
modified: 2015-08-31T03:14:48Z
discontinued: false
dominant: false
experience: experience/None
group u list: _83_
m user id: 0
owner id: 1100
owning group id: 83
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: none
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: spacer from I12007 (26-32)
sequence annotation: CRP binding site
sequence annotation: -10
sequence annotation: spacer from I12007 (77-82)
sequence annotation: spacer from I12007 (54-70)
sequence annotation: spacer from I12007 (1-11, 59-72)
sequence annotation: lam-cI OR2 binding site
sequence: https://synbiohub.org/public/igem/BBa_J58100_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation1908178
display id: annotation1908178
version: 1
top level: pAND
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J58100/annotation1908178/range1908178/1

https://synbiohub.org/public/igem/BBa_J58100/annotation1908178/range1908178/1
type: Range
persistent identity: range1908178
display id: range1908178
version: 1
direction: direction/forward
top level: pAND
start: 72
end: 77
orientation: inline

spacer from I12007 (26-32)
type: SequenceAnnotation
persistent identity: annotation1908180
display id: annotation1908180
version: 1
top level: pAND
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J58100/annotation1908180/range1908180/1

https://synbiohub.org/public/igem/BBa_J58100/annotation1908180/range1908180/1
type: Range
persistent identity: range1908180
display id: range1908180
version: 1
direction: direction/forward
top level: pAND
start: 50
end: 56
orientation: inline

CRP binding site
type: SequenceAnnotation
persistent identity: annotation1908168
display id: annotation1908168
version: 1
top level: pAND
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_J58100/annotation1908168/range1908168/1

https://synbiohub.org/public/igem/BBa_J58100/annotation1908168/range1908168/1
type: Range
persistent identity: range1908168
display id: range1908168
version: 1
direction: direction/forward
top level: pAND
start: 3
end: 24
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1908179
display id: annotation1908179
version: 1
top level: pAND
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J58100/annotation1908179/range1908179/1

https://synbiohub.org/public/igem/BBa_J58100/annotation1908179/range1908179/1
type: Range
persistent identity: range1908179
display id: range1908179
version: 1
direction: direction/forward
top level: pAND
start: 95
end: 100
orientation: inline

spacer from I12007 (77-82)
type: SequenceAnnotation
persistent identity: annotation1908182
display id: annotation1908182
version: 1
top level: pAND
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J58100/annotation1908182/range1908182/1

https://synbiohub.org/public/igem/BBa_J58100/annotation1908182/range1908182/1
type: Range
persistent identity: range1908182
display id: range1908182
version: 1
direction: direction/forward
top level: pAND
start: 101
end: 106
orientation: inline

spacer from I12007 (54-70)
type: SequenceAnnotation
persistent identity: annotation1908181
display id: annotation1908181
version: 1
top level: pAND
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J58100/annotation1908181/range1908181/1

https://synbiohub.org/public/igem/BBa_J58100/annotation1908181/range1908181/1
type: Range
persistent identity: range1908181
display id: range1908181
version: 1
direction: direction/forward
top level: pAND
start: 78
end: 94
orientation: inline

spacer from I12007 (1-11, 59-72)
type: SequenceAnnotation
persistent identity: annotation1908171
display id: annotation1908171
version: 1
top level: pAND
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J58100/annotation1908171/range1908171/1

https://synbiohub.org/public/igem/BBa_J58100/annotation1908171/range1908171/1
type: Range
persistent identity: range1908171
display id: range1908171
version: 1
direction: direction/forward
top level: pAND
start: 25
end: 49
orientation: inline

lam-cI OR2 binding site
type: SequenceAnnotation
persistent identity: annotation1908169
display id: annotation1908169
version: 1
top level: pAND
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_J58100/annotation1908169/range1908169/1

https://synbiohub.org/public/igem/BBa_J58100/annotation1908169/range1908169/1
type: Range
persistent identity: range1908169
display id: range1908169
version: 1
direction: direction/forward
top level: pAND
start: 57
end: 74
orientation: inline

U097O11+D0
type: ComponentDefinition
persistent identity: BBa I732418
display id: BBa_I732418
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U097O11+D062O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732418_sequence/1

BBa_K1125002
type: ComponentDefinition
persistent identity: BBa K1125002
display id: BBa_K1125002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tir1 promoter, Saccharomyces cerevisiae cold shock promoter
creator: Tsung-Yu, Lu
created: 2013-09-15T11:00:00Z
modified: 2015-05-08T01:09:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1437_
m user id: 0
owner id: 17654
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: used to induce the expression of SrUCP under low temperature
mutable notes: presence of an invalid PstI site
mutable provenance: cloned from genomic DNA of Saccharomyces cerevisiae
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1125002_sequence/1

OL
type: ComponentDefinition
persistent identity: BBa K566000
display id: BBa_K566000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: OL region from Lambda
creator: Daniel Rodriguez, Eduardo Almeyda, Porfirio Quintero
created: 2011-09-25T11:00:00Z
modified: 2015-05-08T01:12:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _734_
m user id: 0
owner id: 8650
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: OL region form Lambda phage. It is used to allow a construction to be controlled dually by cI protein. cI protein may interact with pRM promoter and OL region in order to form an octameric structure which permits, first the activation of transcription from pRM promoter and then effectively repression from it as well.
mutable notes: Analysis of illegal restriction sites was performed, but it does not have them originally.
mutable provenance: It comes directly from genomic DNA of Lambda phage. It was extracted by PCR as recommended by the Registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OL
sequence: https://synbiohub.org/public/igem/BBa_K566000_sequence/1

OL
type: SequenceAnnotation
persistent identity: annotation2146174
display id: annotation2146174
version: 1
top level: OL
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K566000/annotation2146174/range2146174/1

https://synbiohub.org/public/igem/BBa_K566000/annotation2146174/range2146174/1
type: Range
persistent identity: range2146174
display id: range2146174
version: 1
direction: direction/forward
top level: OL
start: 1
end: 129
orientation: inline

BBa_K112318
type: ComponentDefinition
persistent identity: BBa K112318
display id: BBa_K112318
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {< bolA promoter>} in BBb format
creator: Aron Lau
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:16Z
discontinued: false
dominant: false
group u list: _224_
m user id: 0
owner id: 2999
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: bolA promoter is a promoter where levels of expression are inversely proportional to the growth rate. To read more, go to following link [http://www.pubmedcentral.nih.gov/picrender.fcgi?artid=402084&blobtype=pdf] This part is actually in BBb format. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: PCR al0017/al0018 on MG1655 (467bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112318 ----------------------------------------------- al0017 Forward Biobricking of bolA promoter CCATAGAATTCatgAGATCTtgctgtggcagtgtaatcgtc al0018 Reverse Biobricking of bolA promoter CGttaGGATCCcttctatccgctcacgtatc
mutable provenance: MG1655
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K112318_sequence/1

U049O11+D0
type: ComponentDefinition
persistent identity: BBa I732405
display id: BBa_I732405
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U049O11+D062NUL)
creator: Zhan Jian
created: 2007-09-19T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1572
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: A Double Reporter (LacZ-alpha and GFP-AAV) loaded on pSB3K5. For more information please refer to BBa_I732402.
mutable notes: Might be later edited.
mutable provenance: Might be later edited.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BamHI
sequence annotation: BBa_I732006
sequence annotation: BBa_I732007
sequence annotation: stop
sequence annotation: polya
sequence annotation: lacZ
sequence annotation: conserved
sequence annotation: mrfp1
sequence annotation: BBa_B0034
sequence annotation: START
sequence annotation: BBa_B0012
sequence annotation: STOP
sequence annotation: BBa_E1010
sequence annotation: stem_loop
sequence annotation: barcode
sequence annotation: T7 TE
sequence annotation: BglII
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_I732405_sequence/1

BamHI
type: SequenceAnnotation
persistent identity: annotation1944405
display id: annotation1944405
version: 1
top level: U049O11+D0
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944405/range1944405/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944405/range1944405/1
type: Range
persistent identity: range1944405
display id: range1944405
version: 1
direction: direction/forward
top level: U049O11+D0
start: 12
end: 17
orientation: inline

BBa_I732006
type: SequenceAnnotation
persistent identity: annotation1944412
display id: annotation1944412
version: 1
top level: U049O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944412/range1944412/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944412/range1944412/1
type: Range
persistent identity: range1944412
display id: range1944412
version: 1
direction: direction/reverse
top level: U049O11+D0
start: 46
end: 279
orientation: inline

BBa_I732007
type: SequenceAnnotation
persistent identity: annotation1944406
display id: annotation1944406
version: 1
top level: U049O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944406/range1944406/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944406/range1944406/1
type: Range
persistent identity: range1944406
display id: range1944406
version: 1
direction: direction/reverse
top level: U049O11+D0
start: 1
end: 19
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1944423
display id: annotation1944423
version: 1
top level: U049O11+D0
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944423/range1944423/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944423/range1944423/1
type: Range
persistent identity: range1944423
display id: range1944423
version: 1
direction: direction/forward
top level: U049O11+D0
start: 1141
end: 1141
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1944422
display id: annotation1944422
version: 1
top level: U049O11+D0
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944422/range1944422/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944422/range1944422/1
type: Range
persistent identity: range1944422
display id: range1944422
version: 1
direction: direction/forward
top level: U049O11+D0
start: 1135
end: 1148
orientation: inline

lacZ
type: SequenceAnnotation
persistent identity: annotation1944410
display id: annotation1944410
version: 1
top level: U049O11+D0
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944410/range1944410/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944410/range1944410/1
type: Range
persistent identity: range1944410
display id: range1944410
version: 1
direction: direction/forward
top level: U049O11+D0
start: 46
end: 279
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation1944413
display id: annotation1944413
version: 1
top level: U049O11+D0
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944413/range1944413/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944413/range1944413/1
type: Range
persistent identity: range1944413
display id: range1944413
version: 1
direction: direction/forward
top level: U049O11+D0
start: 292
end: 295
orientation: inline

mrfp1
type: SequenceAnnotation
persistent identity: annotation1944415
display id: annotation1944415
version: 1
top level: U049O11+D0
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944415/range1944415/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944415/range1944415/1
type: Range
persistent identity: range1944415
display id: range1944415
version: 1
direction: direction/forward
top level: U049O11+D0
start: 306
end: 980
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1944408
display id: annotation1944408
version: 1
top level: U049O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944408/range1944408/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944408/range1944408/1
type: Range
persistent identity: range1944408
display id: range1944408
version: 1
direction: direction/reverse
top level: U049O11+D0
start: 28
end: 39
orientation: inline

START
type: SequenceAnnotation
persistent identity: annotation1944409
display id: annotation1944409
version: 1
top level: U049O11+D0
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944409/range1944409/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944409/range1944409/1
type: Range
persistent identity: range1944409
display id: range1944409
version: 1
direction: direction/forward
top level: U049O11+D0
start: 46
end: 48
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1944420
display id: annotation1944420
version: 1
top level: U049O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944420/range1944420/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944420/range1944420/1
type: Range
persistent identity: range1944420
display id: range1944420
version: 1
direction: direction/forward
top level: U049O11+D0
start: 1108
end: 1148
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1944414
display id: annotation1944414
version: 1
top level: U049O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944414/range1944414/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944414/range1944414/1
type: Range
persistent identity: range1944414
display id: range1944414
version: 1
direction: direction/reverse
top level: U049O11+D0
start: 288
end: 299
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation1944407
display id: annotation1944407
version: 1
top level: U049O11+D0
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944407/range1944407/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944407/range1944407/1
type: Range
persistent identity: range1944407
display id: range1944407
version: 1
direction: direction/forward
top level: U049O11+D0
start: 32
end: 35
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation1944411
display id: annotation1944411
version: 1
top level: U049O11+D0
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944411/range1944411/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944411/range1944411/1
type: Range
persistent identity: range1944411
display id: range1944411
version: 1
direction: direction/forward
top level: U049O11+D0
start: 274
end: 279
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1944416
display id: annotation1944416
version: 1
top level: U049O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944416/range1944416/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944416/range1944416/1
type: Range
persistent identity: range1944416
display id: range1944416
version: 1
direction: direction/reverse
top level: U049O11+D0
start: 306
end: 986
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1944419
display id: annotation1944419
version: 1
top level: U049O11+D0
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944419/range1944419/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944419/range1944419/1
type: Range
persistent identity: range1944419
display id: range1944419
version: 1
direction: direction/forward
top level: U049O11+D0
start: 1031
end: 1074
orientation: inline

barcode
type: SequenceAnnotation
persistent identity: annotation1944417
display id: annotation1944417
version: 1
top level: U049O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944417/range1944417/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944417/range1944417/1
type: Range
persistent identity: range1944417
display id: range1944417
version: 1
direction: direction/reverse
top level: U049O11+D0
start: 987
end: 1011
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1944421
display id: annotation1944421
version: 1
top level: U049O11+D0
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944421/range1944421/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944421/range1944421/1
type: Range
persistent identity: range1944421
display id: range1944421
version: 1
direction: direction/forward
top level: U049O11+D0
start: 1115
end: 1134
orientation: inline

BglII
type: SequenceAnnotation
persistent identity: annotation1944404
display id: annotation1944404
version: 1
top level: U049O11+D0
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944404/range1944404/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944404/range1944404/1
type: Range
persistent identity: range1944404
display id: range1944404
version: 1
direction: direction/forward
top level: U049O11+D0
start: 3
end: 8
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1944418
display id: annotation1944418
version: 1
top level: U049O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944418/range1944418/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944418/range1944418/1
type: Range
persistent identity: range1944418
display id: range1944418
version: 1
direction: direction/forward
top level: U049O11+D0
start: 1020
end: 1099
orientation: inline

BBa_J05216
type: ComponentDefinition
persistent identity: BBa J05216
display id: BBa_J05216
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Regulator for R3-ATF6
creator: Alexander Roth
created: 2005-09-07T11:00:00Z
modified: 2015-08-31T04:08:15Z
discontinued: false
dominant: false
group u list: _34_
m user id: 0
owner id: 412
owning group id: 34
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: binding sites for R1-CREBH and R4-cMaf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J05216_sequence/1

U097NUL+D0
type: ComponentDefinition
persistent identity: BBa I732409
display id: BBa_I732409
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U097NUL+D026O22)
creator: Zhan Jian
created: 2007-09-19T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1572
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: A Double Reporter (LacZ-alpha and GFP) loaded on pSB3C5. For more information please refer to BBa_I732403.
mutable notes: Might be later edited.
mutable provenance: Might be later edited.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_B0034
sequence annotation: stem_loop
sequence annotation: BBa_E0040
sequence annotation: T7 TE
sequence annotation: BamHI
sequence annotation: BBa_B0010
sequence annotation: STOP
sequence annotation: polya
sequence annotation: conserved
sequence annotation: GFP protein
sequence annotation: BBa_I732006
sequence annotation: stop
sequence annotation: lacZ
sequence annotation: BBa_I732007
sequence annotation: START
sequence annotation: BBa_B0012
sequence annotation: BglII
sequence: https://synbiohub.org/public/igem/BBa_I732409_sequence/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1944527
display id: annotation1944527
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944527/range1944527/1

https://synbiohub.org/public/igem/BBa_I732409/annotation1944527/range1944527/1
type: Range
persistent identity: range1944527
display id: range1944527
version: 1
direction: direction/reverse
top level: U097NUL+D0
start: 288
end: 299
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1944521
display id: annotation1944521
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944521/range1944521/1

https://synbiohub.org/public/igem/BBa_I732409/annotation1944521/range1944521/1
type: Range
persistent identity: range1944521
display id: range1944521
version: 1
direction: direction/reverse
top level: U097NUL+D0
start: 28
end: 39
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1944531
display id: annotation1944531
version: 1
top level: U097NUL+D0
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944531/range1944531/1

https://synbiohub.org/public/igem/BBa_I732409/annotation1944531/range1944531/1
type: Range
persistent identity: range1944531
display id: range1944531
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 1045
end: 1088
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1944529
display id: annotation1944529
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944529/range1944529/1

https://synbiohub.org/public/igem/BBa_I732409/annotation1944529/range1944529/1
type: Range
persistent identity: range1944529
display id: range1944529
version: 1
direction: direction/reverse
top level: U097NUL+D0
start: 306
end: 1025
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1944533
display id: annotation1944533
version: 1
top level: U097NUL+D0
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944533/range1944533/1

https://synbiohub.org/public/igem/BBa_I732409/annotation1944533/range1944533/1
type: Range
persistent identity: range1944533
display id: range1944533
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 1129
end: 1148
orientation: inline

BamHI
type: SequenceAnnotation
persistent identity: annotation1944518
display id: annotation1944518
version: 1
top level: U097NUL+D0
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944518/range1944518/1

https://synbiohub.org/public/igem/BBa_I732409/annotation1944518/range1944518/1
type: Range
persistent identity: range1944518
display id: range1944518
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 12
end: 17
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1944530
display id: annotation1944530
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944530/range1944530/1

https://synbiohub.org/public/igem/BBa_I732409/annotation1944530/range1944530/1
type: Range
persistent identity: range1944530
display id: range1944530
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 1034
end: 1113
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation1944524
display id: annotation1944524
version: 1
top level: U097NUL+D0
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944524/range1944524/1

https://synbiohub.org/public/igem/BBa_I732409/annotation1944524/range1944524/1
type: Range
persistent identity: range1944524
display id: range1944524
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 274
end: 279
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1944534
display id: annotation1944534
version: 1
top level: U097NUL+D0
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944534/range1944534/1

https://synbiohub.org/public/igem/BBa_I732409/annotation1944534/range1944534/1
type: Range
persistent identity: range1944534
display id: range1944534
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 1149
end: 1162
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation1944520
display id: annotation1944520
version: 1
top level: U097NUL+D0
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944520/range1944520/1

https://synbiohub.org/public/igem/BBa_I732409/annotation1944520/range1944520/1
type: Range
persistent identity: range1944520
display id: range1944520
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 32
end: 35
orientation: inline

GFP protein
type: SequenceAnnotation
persistent identity: annotation1944528
display id: annotation1944528
version: 1
top level: U097NUL+D0
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944528/range1944528/1

https://synbiohub.org/public/igem/BBa_I732409/annotation1944528/range1944528/1
type: Range
persistent identity: range1944528
display id: range1944528
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 306
end: 1025
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation1944526
display id: annotation1944526
version: 1
top level: U097NUL+D0
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944526/range1944526/1

https://synbiohub.org/public/igem/BBa_I732409/annotation1944526/range1944526/1
type: Range
persistent identity: range1944526
display id: range1944526
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 292
end: 295
orientation: inline

BBa_I732006
type: SequenceAnnotation
persistent identity: annotation1944525
display id: annotation1944525
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944525/range1944525/1

https://synbiohub.org/public/igem/BBa_I732409/annotation1944525/range1944525/1
type: Range
persistent identity: range1944525
display id: range1944525
version: 1
direction: direction/reverse
top level: U097NUL+D0
start: 46
end: 279
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1944535
display id: annotation1944535
version: 1
top level: U097NUL+D0
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944535/range1944535/1

https://synbiohub.org/public/igem/BBa_I732409/annotation1944535/range1944535/1
type: Range
persistent identity: range1944535
display id: range1944535
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 1155
end: 1155
orientation: inline

lacZ
type: SequenceAnnotation
persistent identity: annotation1944523
display id: annotation1944523
version: 1
top level: U097NUL+D0
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944523/range1944523/1

https://synbiohub.org/public/igem/BBa_I732409/annotation1944523/range1944523/1
type: Range
persistent identity: range1944523
display id: range1944523
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 46
end: 279
orientation: inline

BBa_I732007
type: SequenceAnnotation
persistent identity: annotation1944519
display id: annotation1944519
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944519/range1944519/1

https://synbiohub.org/public/igem/BBa_I732409/annotation1944519/range1944519/1
type: Range
persistent identity: range1944519
display id: range1944519
version: 1
direction: direction/reverse
top level: U097NUL+D0
start: 1
end: 19
orientation: inline

START
type: SequenceAnnotation
persistent identity: annotation1944522
display id: annotation1944522
version: 1
top level: U097NUL+D0
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944522/range1944522/1

https://synbiohub.org/public/igem/BBa_I732409/annotation1944522/range1944522/1
type: Range
persistent identity: range1944522
display id: range1944522
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 46
end: 48
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1944532
display id: annotation1944532
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944532/range1944532/1

https://synbiohub.org/public/igem/BBa_I732409/annotation1944532/range1944532/1
type: Range
persistent identity: range1944532
display id: range1944532
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 1122
end: 1162
orientation: inline

BglII
type: SequenceAnnotation
persistent identity: annotation1944517
display id: annotation1944517
version: 1
top level: U097NUL+D0
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944517/range1944517/1

https://synbiohub.org/public/igem/BBa_I732409/annotation1944517/range1944517/1
type: Range
persistent identity: range1944517
display id: range1944517
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 3
end: 8
orientation: inline

BBa_K112322
type: ComponentDefinition
persistent identity: BBa K112322
display id: BBa_K112322
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {Pdps} in BBb format
creator: Aron Lau
created: 2008-10-21T11:00:00Z
modified: 2015-05-08T01:09:16Z
discontinued: false
dominant: false
group u list: _224_
m user id: 0
owner id: 2999
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Pdps promoter. This part is actually in BBb format. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: PCR JH089F/JH089R on Salmonella L2. (379 bp, EcoRI/BamHI/DpnI) Sub into pBjh1601CK (EcoRI/BamHI, 3134+910, L) Product is pBjh1601CK-K112322 ----------------------------------------------- JH089F Forward biobricking of {Pdps} cgataGAATTCatgAGATCTaacgattcgacgcgaaccgc JH089R Reverse biobricking of {Pdps} cgttaGGATCCaatctcatatcctcttgatg
mutable provenance: Salmonella L2
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K112322_sequence/1

U061O11+D0
type: ComponentDefinition
persistent identity: BBa I732433
display id: BBa_I732433
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U061O11+D026O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732433_sequence/1

BBa_K091146
type: ComponentDefinition
persistent identity: BBa K091146
display id: BBa_K091146
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLas/Lux Hybrid Promoter
creator: Erin Feeney
created: 2008-07-16T11:00:00Z
modified: 2015-05-08T01:08:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _191_
m user id: 0
owner id: 3067
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: K091146 is a modified version of the pLas' promoter (BBa_K091117) containing the addition of a luxR binding site between the -35 and -10 regions. This promoter is expected to be activated in the presence of PAI-1 and LasR and repressed in the presence of AI-1 and LuxR.
mutable notes: The Lux box was placed adjacent to the LasR binding site with the aim of minimizing cross-talk in the presence of both PAI-1 and AI-1; since the LasR site should have a higher affinity, we hope that the binding of PAI-1 will competitively exclude the binding of AI-1.
mutable provenance: The LuxR binding site is from BBa_R0062 and the pLas' promoter is from the region upstream of the LasI gene in Pseudomonas aeruginosa.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: LuxR
sequence annotation: LasR
sequence annotation: -35
sequence annotation: -10
sequence annotation: Transcriptional Start
sequence: https://synbiohub.org/public/igem/BBa_K091146_sequence/1

LuxR
type: SequenceAnnotation
persistent identity: annotation1967355
display id: annotation1967355
version: 1
top level: BBa_K091146
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K091146/annotation1967355/range1967355/1

https://synbiohub.org/public/igem/BBa_K091146/annotation1967355/range1967355/1
type: Range
persistent identity: range1967355
display id: range1967355
version: 1
direction: direction/forward
top level: BBa_K091146
start: 94
end: 113
orientation: inline

LasR
type: SequenceAnnotation
persistent identity: annotation1967354
display id: annotation1967354
version: 1
top level: BBa_K091146
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K091146/annotation1967354/range1967354/1

https://synbiohub.org/public/igem/BBa_K091146/annotation1967354/range1967354/1
type: Range
persistent identity: range1967354
display id: range1967354
version: 1
direction: direction/forward
top level: BBa_K091146
start: 75
end: 93
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1967357
display id: annotation1967357
version: 1
top level: BBa_K091146
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K091146/annotation1967357/range1967357/1

https://synbiohub.org/public/igem/BBa_K091146/annotation1967357/range1967357/1
type: Range
persistent identity: range1967357
display id: range1967357
version: 1
direction: direction/forward
top level: BBa_K091146
start: 86
end: 91
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1967356
display id: annotation1967356
version: 1
top level: BBa_K091146
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K091146/annotation1967356/range1967356/1

https://synbiohub.org/public/igem/BBa_K091146/annotation1967356/range1967356/1
type: Range
persistent identity: range1967356
display id: range1967356
version: 1
direction: direction/forward
top level: BBa_K091146
start: 113
end: 118
orientation: inline

Transcriptional Start
type: SequenceAnnotation
persistent identity: annotation1967358
display id: annotation1967358
version: 1
top level: BBa_K091146
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K091146/annotation1967358/range1967358/1

https://synbiohub.org/public/igem/BBa_K091146/annotation1967358/range1967358/1
type: Range
persistent identity: range1967358
display id: range1967358
version: 1
direction: direction/forward
top level: BBa_K091146
start: 125
end: 125
orientation: inline

U073O11+D0
type: ComponentDefinition
persistent identity: BBa I732427
display id: BBa_I732427
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U073O11+D026O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732427_sequence/1

BBa_K091106
type: ComponentDefinition
persistent identity: BBa K091106
display id: BBa_K091106
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LsrA/cI hybrid promoter
creator: Andrew Gordon
created: 2008-06-18T11:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _191_
m user id: 0
owner id: 3129
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This hybrid promoter uses the LsrA promoter that is capable of being repressed by LsrR - induction can occur with phospho-AI-2. Binding sites for cI repressor also occur. As a result the promoter is off in the absence of AI-2 and on in the presence of AI-2, but always off in the presence of cI.
mutable notes: The promoter region for LsrA should remain unaltered while cI binding sites (OR1 and OR2) are introduced downstream. The OR2 sites with cI bound should not allow transcription. The cI binding sites have a natural 6 bp spacer between them. It would be desirable to position the cI binding sites immediately downstream of the -10 region of the LsrA promoter. However, experimental evidence for the transciption start (+1) appears to be lacking.
mutable provenance: The Missouri Western/Davidson team designed primers for LsrA and extracted the DNA from E. coli MG1655. The CI portion was pulled from the registry as part number R0051.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K091106_sequence/1

BBa_K091157
type: ComponentDefinition
persistent identity: BBa K091157
display id: BBa_K091157
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLux/Las Hybrid Promoter
creator: Erin Feeney
created: 2008-07-24T11:00:00Z
modified: 2015-05-08T01:08:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _191_
m user id: 0
owner id: 3067
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: K091157 is a modified version of the pLux' promoter (BBa_K091156) containing the addition of a LasR binding site between the -35 and -10 regions. This promoter is expected to be activated in the presence of AI-1 and LuxR and repressed in the presence of PAI-1 and LasR.
mutable notes: In order to fit the LasR binding site between the -35 and -10 regions, the last base of the Las Box was cropped and the -35 region was modified to overlap with the first two bases of the Las Box.
mutable provenance: The LasR binding site is from the region upstream of the LasI gene in Pseudomonas aeruginosa. The rest of the promoter is the same as BBa_K091156.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10 Region
sequence annotation: LasR + PAI-1
sequence annotation: LuxR+AI-1
sequence annotation: -35 Region
sequence: https://synbiohub.org/public/igem/BBa_K091157_sequence/1

-10 Region
type: SequenceAnnotation
persistent identity: annotation1968424
display id: annotation1968424
version: 1
top level: BBa_K091157
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K091157/annotation1968424/range1968424/1

https://synbiohub.org/public/igem/BBa_K091157/annotation1968424/range1968424/1
type: Range
persistent identity: range1968424
display id: range1968424
version: 1
direction: direction/forward
top level: BBa_K091157
start: 42
end: 47
orientation: inline

LasR + PAI-1
type: SequenceAnnotation
persistent identity: annotation1968425
display id: annotation1968425
version: 1
top level: BBa_K091157
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K091157/annotation1968425/range1968425/1

https://synbiohub.org/public/igem/BBa_K091157/annotation1968425/range1968425/1
type: Range
persistent identity: range1968425
display id: range1968425
version: 1
direction: direction/forward
top level: BBa_K091157
start: 24
end: 42
orientation: inline

LuxR+AI-1
type: SequenceAnnotation
persistent identity: annotation1968426
display id: annotation1968426
version: 1
top level: BBa_K091157
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K091157/annotation1968426/range1968426/1

https://synbiohub.org/public/igem/BBa_K091157/annotation1968426/range1968426/1
type: Range
persistent identity: range1968426
display id: range1968426
version: 1
direction: direction/forward
top level: BBa_K091157
start: 1
end: 20
orientation: inline

-35 Region
type: SequenceAnnotation
persistent identity: annotation1968423
display id: annotation1968423
version: 1
top level: BBa_K091157
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K091157/annotation1968423/range1968423/1

https://synbiohub.org/public/igem/BBa_K091157/annotation1968423/range1968423/1
type: Range
persistent identity: range1968423
display id: range1968423
version: 1
direction: direction/forward
top level: BBa_K091157
start: 20
end: 25
orientation: inline

BBa_K283018
type: ComponentDefinition
persistent identity: BBa K283018
display id: BBa_K283018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FF-FF ansB promoter -rbs
creator: Shi Lei, W Ling , Nelson Chu
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:47Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _383_
m user id: 0
owner id: 5494
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: panas promoter with a rbs. The panas promoter is hypoxia inducible.
mutable notes: BBa_K283018
mutable provenance: The panas promoter is self-designed The rbs is from the registry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K283031
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_K283031
sequence: https://synbiohub.org/public/igem/BBa_K283018_sequence/1

BBa_K283031
type: Component
persistent identity: component2047407
display id: component2047407
version: 1
top level: BBa_K283018
access: public
definition: https://synbiohub.org/public/igem/BBa_K283031/1

BBa_B0034
type: Component
persistent identity: component2047409
display id: component2047409
version: 1
top level: BBa_K283018
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2047409
display id: annotation2047409
version: 1
top level: BBa_K283018
location: https://synbiohub.org/public/igem/BBa_K283018/annotation2047409/range2047409/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K283018/annotation2047409/range2047409/1
type: Range
persistent identity: range2047409
display id: range2047409
version: 1
direction: direction/reverse
top level: BBa_K283018
start: 114
end: 125
orientation: inline

BBa_K283031
type: SequenceAnnotation
persistent identity: annotation2047407
display id: annotation2047407
version: 1
top level: BBa_K283018
location: https://synbiohub.org/public/igem/BBa_K283018/annotation2047407/range2047407/1
component: BBa_K283031

https://synbiohub.org/public/igem/BBa_K283018/annotation2047407/range2047407/1
type: Range
persistent identity: range2047407
display id: range2047407
version: 1
direction: direction/reverse
top level: BBa_K283018
start: 1
end: 105
orientation: inline

nitA promo
type: ComponentDefinition
persistent identity: BBa K836008
display id: BBa_K836008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: nitA promoter
creator: Ricardo Alvarado & Veronica Delgado
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1095_
m user id: 0
owner id: 11819
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Promoter inducible by nitrile and similar agents.
mutable notes: The sequence was used as reported.
mutable provenance: Promoter taken from NCBI (http://www.ncbi.nlm.nih.gov/nucleotide/346420896?report=genbank&log$=nuclalign&blast_rank=1&RID=W0SH1PA7016).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: nitA promoter
sequence: https://synbiohub.org/public/igem/BBa_K836008_sequence/1

nitA promoter
type: SequenceAnnotation
persistent identity: annotation2187537
display id: annotation2187537
version: 1
top level: nitA promo
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K836008/annotation2187537/range2187537/1

https://synbiohub.org/public/igem/BBa_K836008/annotation2187537/range2187537/1
type: Range
persistent identity: range2187537
display id: range2187537
version: 1
direction: direction/forward
top level: nitA promo
start: 1
end: 115
orientation: inline

U097O11+D0
type: ComponentDefinition
persistent identity: BBa I732414
display id: BBa_I732414
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U097O11+D014O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732414_sequence/1

BBa_I741018
type: ComponentDefinition
persistent identity: BBa I741018
display id: BBa_I741018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Right facing promoter (for xylF) controlled by xylR and CRP-cAMP
creator: Lucien Weiss
created: 2007-06-20T11:00:00Z
modified: 2015-08-31T04:08:01Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _143_
m user id: 0
owner id: 1763
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: potential looping feature possibly removed
mutable notes: removed RBS, left promoter, and several xylR binding sites
mutable provenance: ecoli k12 chromosome
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Promoter: xylFp
sequence annotation: xylR activator binding site
sequence annotation: CRP-cAMP duel regulator binding site
sequence annotation: +1
sequence: https://synbiohub.org/public/igem/BBa_I741018_sequence/1

Promoter: xylFp
type: SequenceAnnotation
persistent identity: annotation1935200
display id: annotation1935200
version: 1
top level: BBa_I741018
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I741018/annotation1935200/range1935200/1

https://synbiohub.org/public/igem/BBa_I741018/annotation1935200/range1935200/1
type: Range
persistent identity: range1935200
display id: range1935200
version: 1
direction: direction/forward
top level: BBa_I741018
start: 138
end: 215
orientation: inline

xylR activator binding site
type: SequenceAnnotation
persistent identity: annotation1935199
display id: annotation1935199
version: 1
top level: BBa_I741018
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I741018/annotation1935199/range1935199/1

https://synbiohub.org/public/igem/BBa_I741018/annotation1935199/range1935199/1
type: Range
persistent identity: range1935199
display id: range1935199
version: 1
direction: direction/forward
top level: BBa_I741018
start: 125
end: 142
orientation: inline

CRP-cAMP duel regulator binding site
type: SequenceAnnotation
persistent identity: annotation1935203
display id: annotation1935203
version: 1
top level: BBa_I741018
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I741018/annotation1935203/range1935203/1

https://synbiohub.org/public/igem/BBa_I741018/annotation1935203/range1935203/1
type: Range
persistent identity: range1935203
display id: range1935203
version: 1
direction: direction/forward
top level: BBa_I741018
start: 4
end: 25
orientation: inline

xylR activator binding site
type: SequenceAnnotation
persistent identity: annotation1935201
display id: annotation1935201
version: 1
top level: BBa_I741018
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I741018/annotation1935201/range1935201/1

https://synbiohub.org/public/igem/BBa_I741018/annotation1935201/range1935201/1
type: Range
persistent identity: range1935201
display id: range1935201
version: 1
direction: direction/forward
top level: BBa_I741018
start: 146
end: 162
orientation: inline

+1
type: SequenceAnnotation
persistent identity: annotation1935202
display id: annotation1935202
version: 1
top level: BBa_I741018
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I741018/annotation1935202/range1935202/1

https://synbiohub.org/public/igem/BBa_I741018/annotation1935202/range1935202/1
type: Range
persistent identity: range1935202
display id: range1935202
version: 1
direction: direction/forward
top level: BBa_I741018
start: 195
end: 195
orientation: inline

NAND2717
type: ComponentDefinition
persistent identity: BBa I732302
display id: BBa_I732302
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NAND Candidate (U073O27D001O17)
creator: Ma Rui
created: 2007-10-02T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _156_
m user id: 0
owner id: 1592
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: U97NUL D001O22
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732302_sequence/1

BBa_K091105
type: ComponentDefinition
persistent identity: BBa K091105
display id: BBa_K091105
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pTet/Mnt Hybrid Promoter
creator: Robert Cool
created: 2008-06-18T11:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _191_
m user id: 0
owner id: 3080
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This promoter is a modified version of the Mnt promoter that is also responsive to TetR. The promoter should be repressed by Mnt repressor. It should also be repressed by TetR, and in the absence of Mnt repressor, should be induced by aTc.
mutable notes: Mnt repressor binds as a tetramer to two half-operator sites. Introduction of TetR binding sites between the Mnt promoter -10 sequence and the start site for transcription should allow for repression by TetR. Accordingly, the hybrid promoter was designed by 1) Remove all bases of mnt promoter 3??? to -10: gagtcgtattaattt will be replaced by tccctatcagtgata, and 2) truncate the second half of ptet -10 and mutate -35 so that it function to bind tetR (replace ttgaca with actgta), but is not a promoter, and 3) place the tetR1 and tetR2 binding sites 3??? to truncated mnt -10 sequence.
mutable provenance: This part comes from the Mnt promoter and the TetR promoter
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: tetR2
sequence annotation: tetR1
sequence annotation: Mnt
sequence: https://synbiohub.org/public/igem/BBa_K091105_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation1963739
display id: annotation1963739
version: 1
top level: BBa_K091105
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K091105/annotation1963739/range1963739/1

https://synbiohub.org/public/igem/BBa_K091105/annotation1963739/range1963739/1
type: Range
persistent identity: range1963739
display id: range1963739
version: 1
direction: direction/forward
top level: BBa_K091105
start: 47
end: 52
orientation: inline

tetR2
type: SequenceAnnotation
persistent identity: annotation1963741
display id: annotation1963741
version: 1
top level: BBa_K091105
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K091105/annotation1963741/range1963741/1

https://synbiohub.org/public/igem/BBa_K091105/annotation1963741/range1963741/1
type: Range
persistent identity: range1963741
display id: range1963741
version: 1
direction: direction/forward
top level: BBa_K091105
start: 78
end: 98
orientation: inline

tetR1
type: SequenceAnnotation
persistent identity: annotation1963740
display id: annotation1963740
version: 1
top level: BBa_K091105
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K091105/annotation1963740/range1963740/1

https://synbiohub.org/public/igem/BBa_K091105/annotation1963740/range1963740/1
type: Range
persistent identity: range1963740
display id: range1963740
version: 1
direction: direction/forward
top level: BBa_K091105
start: 53
end: 71
orientation: inline

Mnt
type: SequenceAnnotation
persistent identity: annotation1963742
display id: annotation1963742
version: 1
top level: BBa_K091105
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K091105/annotation1963742/range1963742/1

https://synbiohub.org/public/igem/BBa_K091105/annotation1963742/range1963742/1
type: Range
persistent identity: range1963742
display id: range1963742
version: 1
direction: direction/forward
top level: BBa_K091105
start: 1
end: 52
orientation: inline

U037O11+D0
type: ComponentDefinition
persistent identity: BBa I732447
display id: BBa_I732447
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U037O11+D050O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732447_sequence/1

BBa_K165034
type: ComponentDefinition
persistent identity: BBa K165034
display id: BBa_K165034
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Zif268-HIV bs + LexA bs + mCYC promoter
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:56Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The Zif268-HIV and LexA transcription factor binding sites on the mCYC minimal promoter (in that order.) Transcription regulation comes from Zif268-HIV and LexA-binding transcription factors.
mutable notes: -
mutable provenance: Constructed in our lab with parts from Caroline Ajo-Franklin and David Drubin.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K165011
component: BBa_K165031
sequence annotation: BBa_K165031
sequence annotation: BBa_K165011
sequence: https://synbiohub.org/public/igem/BBa_K165034_sequence/1

BBa_K165011
type: Component
persistent identity: component1995464
display id: component1995464
version: 1
top level: BBa_K165034
access: public
definition: https://synbiohub.org/public/igem/BBa_K165011/1

BBa_K165031
type: Component
persistent identity: component1995465
display id: component1995465
version: 1
top level: BBa_K165034
access: public
definition: https://synbiohub.org/public/igem/BBa_K165031/1

BBa_K165031
type: SequenceAnnotation
persistent identity: annotation1995465
display id: annotation1995465
version: 1
top level: BBa_K165034
location: https://synbiohub.org/public/igem/BBa_K165034/annotation1995465/range1995465/1
component: BBa_K165031

https://synbiohub.org/public/igem/BBa_K165034/annotation1995465/range1995465/1
type: Range
persistent identity: range1995465
display id: range1995465
version: 1
direction: direction/reverse
top level: BBa_K165034
start: 55
end: 457
orientation: inline

BBa_K165011
type: SequenceAnnotation
persistent identity: annotation1995464
display id: annotation1995464
version: 1
top level: BBa_K165034
location: https://synbiohub.org/public/igem/BBa_K165034/annotation1995464/range1995464/1
component: BBa_K165011

https://synbiohub.org/public/igem/BBa_K165034/annotation1995464/range1995464/1
type: Range
persistent identity: range1995464
display id: range1995464
version: 1
direction: direction/reverse
top level: BBa_K165034
start: 1
end: 46
orientation: inline

BBa_I732450
type: ComponentDefinition
persistent identity: BBa I732450
display id: BBa_I732450
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U073O26+D062NUL)
creator: Zhan Jian
created: 2007-10-16T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732450_sequence/1

U061O11+D0
type: ComponentDefinition
persistent identity: BBa I732432
display id: BBa_I732432
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U061O11+D014O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732432_sequence/1

BBa_I761011
type: ComponentDefinition
persistent identity: BBa I761011
display id: BBa_I761011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CinR, CinL and glucose controlled promotor
creator: 2007 NYMU_Taiwan iGEM Team
created: 2007-10-18T11:00:00Z
modified: 2015-08-31T04:08:08Z
discontinued: false
dominant: false
experience: experience/None
group u list: _140_
m user id: 0
owner id: 2031
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This promotor is controlled by cinr, cinl and extrcellular glucose concentration. High extracellular glucose concentration will inhibit this promotor through the action of phosphorylated OmpR. Phosphorylated OmpR will bind the OmpR binding site of the promotor and block the movement of RNA polymerase. In contrast, the combined action of CinR and CinL will turn on the promotor. But only when the extracellular glucose level is low so OmpR dephosphorylate and dissociate from its binding site will this promotor become active. In essence, this promotor function as an AND gate, it will turn on if and only if the extracellular glucose level is low and there are some functional CinR and CinL protein inside the cell.
mutable notes: The key to the function of the promotor is the inhibitory ability of OmpR binding site. To ensure maximum affinity with phosphorylated OmpR, we use all three OmpR binding site.s.
mutable provenance: *BBa_R0078 *BBa_I761001
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_R0078
component: BBa_I761001
sequence annotation: BBa_R0078
sequence annotation: BBa_I761001
sequence: https://synbiohub.org/public/igem/BBa_I761011_sequence/1

BBa_R0078
type: Component
persistent identity: component1950118
display id: component1950118
version: 1
top level: BBa_I761011
access: public
definition: https://synbiohub.org/public/igem/BBa_R0078/1

BBa_I761001
type: Component
persistent identity: component1950122
display id: component1950122
version: 1
top level: BBa_I761011
access: public
definition: https://synbiohub.org/public/igem/BBa_I761001/1

BBa_R0078
type: SequenceAnnotation
persistent identity: annotation1950118
display id: annotation1950118
version: 1
top level: BBa_I761011
location: https://synbiohub.org/public/igem/BBa_I761011/annotation1950118/range1950118/1
component: BBa_R0078

https://synbiohub.org/public/igem/BBa_I761011/annotation1950118/range1950118/1
type: Range
persistent identity: range1950118
display id: range1950118
version: 1
direction: direction/reverse
top level: BBa_I761011
start: 1
end: 225
orientation: inline

BBa_I761001
type: SequenceAnnotation
persistent identity: annotation1950122
display id: annotation1950122
version: 1
top level: BBa_I761011
location: https://synbiohub.org/public/igem/BBa_I761011/annotation1950122/range1950122/1
component: BBa_I761001

https://synbiohub.org/public/igem/BBa_I761011/annotation1950122/range1950122/1
type: Range
persistent identity: range1950122
display id: range1950122
version: 1
direction: direction/reverse
top level: BBa_I761011
start: 234
end: 295
orientation: inline

BBa_K993002
type: ComponentDefinition
persistent identity: BBa K993002
display id: BBa_K993002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IbpB RNA Thermometer with RFP
creator: İBRAHİM YASİR ORHAN
created: 2013-06-13T11:00:00Z
modified: 2015-05-08T01:13:51Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1290_
m user id: 0
owner id: 11153
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: The IbpB thermometer is an RNA thermometer element found in the ibpAB operon. The operon contains two heat-shock genes, encoding inclusion body binding proteins A and B (IbpA/B), and is the most drastically upregulated operon under heat-shock in Escherichia coli. IbpA is regulated by a ROSE element found in its 5' UTR, while IbpB has its own heat-sensitive cis-regulatory element. The activity of this thermoregulator was confirmed in vitro but was not found in vivo, suggesting more complicated operon regulation exists in bacterial cells. In this part, red fluorescent protein (RFP) is added in the downstream region to measure the activity of RNA thermometer. You check our Experience page for further information about our characterization and experiments. For the inner elements in the part design; check out the Part Design page. RNA Thermometers are the only known single-component regulators of gene expression.(1) RNA Thermometers are thermosensors that regulate gene expression by temperature-induced changes in RNA conformation. Naturally occurring RNA Thermometers exhibit complex secondary structures which are believed to undergo a series of gradual structural changes in response to temperature shifts.(2) RNA Thermometers often regulate genes required during either a heat shock or cold shock response, but have been implicated in other regulatory roles such as in pathogenecity and starvation.(3)
mutable notes: Note that RNA thermometer actually works as an RBS sequence. Therefore, this part does not include an additional ribosome binding site. Also, this part does not contain an terminator sequence, but a simple stop codon. The RFP should be translated continuously.
mutable provenance: Escherichia coli, NCBI GeneBank Other elements are obtained from Registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: Stop codon
sequence annotation: pVeg2
sequence annotation: Start codon
sequence annotation: IbpB thermometer
sequence annotation: RFP
sequence: https://synbiohub.org/public/igem/BBa_K993002_sequence/1

Stop codon
type: SequenceAnnotation
persistent identity: annotation2329445
display id: annotation2329445
version: 1
top level: BBa_K993002
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K993002/annotation2329445/range2329445/1

https://synbiohub.org/public/igem/BBa_K993002/annotation2329445/range2329445/1
type: Range
persistent identity: range2329445
display id: range2329445
version: 1
direction: direction/forward
top level: BBa_K993002
start: 915
end: 917
orientation: inline

pVeg2
type: SequenceAnnotation
persistent identity: annotation2329442
display id: annotation2329442
version: 1
top level: BBa_K993002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K993002/annotation2329442/range2329442/1

https://synbiohub.org/public/igem/BBa_K993002/annotation2329442/range2329442/1
type: Range
persistent identity: range2329442
display id: range2329442
version: 1
direction: direction/forward
top level: BBa_K993002
start: 1
end: 97
orientation: inline

Start codon
type: SequenceAnnotation
persistent identity: annotation2329446
display id: annotation2329446
version: 1
top level: BBa_K993002
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K993002/annotation2329446/range2329446/1

https://synbiohub.org/public/igem/BBa_K993002/annotation2329446/range2329446/1
type: Range
persistent identity: range2329446
display id: range2329446
version: 1
direction: direction/forward
top level: BBa_K993002
start: 209
end: 211
orientation: inline

IbpB thermometer
type: SequenceAnnotation
persistent identity: annotation2329443
display id: annotation2329443
version: 1
top level: BBa_K993002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K993002/annotation2329443/range2329443/1

https://synbiohub.org/public/igem/BBa_K993002/annotation2329443/range2329443/1
type: Range
persistent identity: range2329443
display id: range2329443
version: 1
direction: direction/forward
top level: BBa_K993002
start: 98
end: 208
orientation: inline

RFP
type: SequenceAnnotation
persistent identity: annotation2329444
display id: annotation2329444
version: 1
top level: BBa_K993002
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K993002/annotation2329444/range2329444/1

https://synbiohub.org/public/igem/BBa_K993002/annotation2329444/range2329444/1
type: Range
persistent identity: range2329444
display id: range2329444
version: 1
direction: direction/forward
top level: BBa_K993002
start: 209
end: 917
orientation: inline

BBa_J05215
type: ComponentDefinition
persistent identity: BBa J05215
display id: BBa_J05215
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Regulator for R1-CREBH
creator: Alexander Roth
created: 2005-09-07T11:00:00Z
modified: 2015-08-31T04:08:15Z
discontinued: false
dominant: false
group u list: _34_
m user id: 0
owner id: 412
owning group id: 34
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: binding sites for R3-ATF6 and R2-YAP7
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J05215_sequence/1

U085O11+D0
type: ComponentDefinition
persistent identity: BBa I732421
display id: BBa_I732421
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U085O11+D026O22)
creator: Zhan Jian
created: 2007-10-02T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1592
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Our first NOR logical gate
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732421_sequence/1

BBa_K1486011
type: ComponentDefinition
persistent identity: BBa K1486011
display id: BBa_K1486011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CxpR & Split IFP1.4 [Cterm + Nterm]
creator: iGem EPFL 2014
created: 2014-08-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part has two fused proteins downstream of an arabinose-inducible promoter: CpxR-IFP[1] and CpxR-IFP[2]. This construct was made in all orientations: - CpxR-IFP[1] + CpxR-IFP[2] (BBa_K1286008) - IFP[1]-CpxR + CpxR-IFP[2] (BBa_K1486010) - CpxR-IFP[1] + IFP[2]-CpxR (BBa_K1486011)
mutable notes: All orientations were made, so all dimerizations could be tested (see main page for biobrick numbers). Flexible linkers were used for each of the fused proteins, and an RBS was put before each one of them.
mutable provenance: From Michnick Article. To Edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_K1486004
component: BBa_K1486006
component: BBa_K1486000
component: BBa_K1486007
component: BBa_I0500
sequence annotation: BBa_K1486007
sequence annotation: BBa_K1486006
sequence annotation: BBa_K1486004
sequence annotation: BBa_B0034
sequence annotation: BBa_K1486000
sequence annotation: BBa_I0500
sequence: https://synbiohub.org/public/igem/BBa_K1486011_sequence/1

BBa_B0034
type: Component
persistent identity: component2382245
display id: component2382245
version: 1
top level: BBa_K1486011
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486004
type: Component
persistent identity: component2382250
display id: component2382250
version: 1
top level: BBa_K1486011
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_K1486006
type: Component
persistent identity: component2382243
display id: component2382243
version: 1
top level: BBa_K1486011
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486006/1

BBa_B0034
type: Component
persistent identity: component2382237
display id: component2382237
version: 1
top level: BBa_K1486011
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486000
type: Component
persistent identity: component2382254
display id: component2382254
version: 1
top level: BBa_K1486011
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_K1486007
type: Component
persistent identity: component2382249
display id: component2382249
version: 1
top level: BBa_K1486011
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486007/1

BBa_K1486004
type: Component
persistent identity: component2382242
display id: component2382242
version: 1
top level: BBa_K1486011
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_I0500
type: Component
persistent identity: component2382235
display id: component2382235
version: 1
top level: BBa_K1486011
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_K1486000
type: Component
persistent identity: component2382241
display id: component2382241
version: 1
top level: BBa_K1486011
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_K1486007
type: SequenceAnnotation
persistent identity: annotation2382249
display id: annotation2382249
version: 1
top level: BBa_K1486011
location: https://synbiohub.org/public/igem/BBa_K1486011/annotation2382249/range2382249/1
component: BBa_K1486007

https://synbiohub.org/public/igem/BBa_K1486011/annotation2382249/range2382249/1
type: Range
persistent identity: range2382249
display id: range2382249
version: 1
direction: direction/reverse
top level: BBa_K1486011
start: 2549
end: 3127
orientation: inline

BBa_K1486006
type: SequenceAnnotation
persistent identity: annotation2382243
display id: annotation2382243
version: 1
top level: BBa_K1486011
location: https://synbiohub.org/public/igem/BBa_K1486011/annotation2382243/range2382243/1
component: BBa_K1486006

https://synbiohub.org/public/igem/BBa_K1486011/annotation2382243/range2382243/1
type: Range
persistent identity: range2382243
display id: range2382243
version: 1
direction: direction/reverse
top level: BBa_K1486011
start: 1955
end: 2536
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2382250
display id: annotation2382250
version: 1
top level: BBa_K1486011
location: https://synbiohub.org/public/igem/BBa_K1486011/annotation2382250/range2382250/1
component: BBa_K1486004

https://synbiohub.org/public/igem/BBa_K1486011/annotation2382250/range2382250/1
type: Range
persistent identity: range2382250
display id: range2382250
version: 1
direction: direction/reverse
top level: BBa_K1486011
start: 3128
end: 3157
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2382237
display id: annotation2382237
version: 1
top level: BBa_K1486011
location: https://synbiohub.org/public/igem/BBa_K1486011/annotation2382237/range2382237/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486011/annotation2382237/range2382237/1
type: Range
persistent identity: range2382237
display id: range2382237
version: 1
direction: direction/reverse
top level: BBa_K1486011
start: 1211
end: 1222
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2382241
display id: annotation2382241
version: 1
top level: BBa_K1486011
location: https://synbiohub.org/public/igem/BBa_K1486011/annotation2382241/range2382241/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486011/annotation2382241/range2382241/1
type: Range
persistent identity: range2382241
display id: range2382241
version: 1
direction: direction/reverse
top level: BBa_K1486011
start: 1223
end: 1924
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2382245
display id: annotation2382245
version: 1
top level: BBa_K1486011
location: https://synbiohub.org/public/igem/BBa_K1486011/annotation2382245/range2382245/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486011/annotation2382245/range2382245/1
type: Range
persistent identity: range2382245
display id: range2382245
version: 1
direction: direction/reverse
top level: BBa_K1486011
start: 2537
end: 2548
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2382254
display id: annotation2382254
version: 1
top level: BBa_K1486011
location: https://synbiohub.org/public/igem/BBa_K1486011/annotation2382254/range2382254/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486011/annotation2382254/range2382254/1
type: Range
persistent identity: range2382254
display id: range2382254
version: 1
direction: direction/reverse
top level: BBa_K1486011
start: 3158
end: 3859
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2382235
display id: annotation2382235
version: 1
top level: BBa_K1486011
location: https://synbiohub.org/public/igem/BBa_K1486011/annotation2382235/range2382235/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486011/annotation2382235/range2382235/1
type: Range
persistent identity: range2382235
display id: range2382235
version: 1
direction: direction/reverse
top level: BBa_K1486011
start: 1
end: 1210
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2382242
display id: annotation2382242
version: 1
top level: BBa_K1486011
location: https://synbiohub.org/public/igem/BBa_K1486011/annotation2382242/range2382242/1
component: BBa_K1486004

https://synbiohub.org/public/igem/BBa_K1486011/annotation2382242/range2382242/1
type: Range
persistent identity: range2382242
display id: range2382242
version: 1
direction: direction/reverse
top level: BBa_K1486011
start: 1925
end: 1954
orientation: inline

U061O11+D0
type: ComponentDefinition
persistent identity: BBa I732435
display id: BBa_I732435
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U061O11+D050O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732435_sequence/1

U097O11+D0
type: ComponentDefinition
persistent identity: BBa I732401
display id: BBa_I732401
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U097O11+D062NUL)
creator: Zhan Jian
created: 2007-10-02T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1592
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Our first NAND logical gate
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732401_sequence/1

U073O11+D0
type: ComponentDefinition
persistent identity: BBa I732428
display id: BBa_I732428
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U073O11+D038O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732428_sequence/1

U061O11+D0
type: ComponentDefinition
persistent identity: BBa I732404
display id: BBa_I732404
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U061O11+D062NUL)
creator: Zhan Jian
created: 2007-09-19T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1572
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: A Double Reporter (LacZ-alpha and GFP-AAV) loaded on pSB3K5. For more information please refer to BBa_I732401.
mutable notes: Might be later edited.
mutable provenance: Might be later edited.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: START
sequence annotation: BBa_B0034
sequence annotation: stop
sequence annotation: polya
sequence annotation: lacZ
sequence annotation: BBa_I732006
sequence annotation: conserved
sequence annotation: T7 TE
sequence annotation: BglII
sequence annotation: BamHI
sequence annotation: STOP
sequence annotation: BBa_E0044
sequence annotation: stem_loop
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_I732404_sequence/1

START
type: SequenceAnnotation
persistent identity: annotation1947690
display id: annotation1947690
version: 1
top level: U061O11+D0
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947690/range1947690/1

https://synbiohub.org/public/igem/BBa_I732404/annotation1947690/range1947690/1
type: Range
persistent identity: range1947690
display id: range1947690
version: 1
direction: direction/forward
top level: U061O11+D0
start: 46
end: 48
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1947692
display id: annotation1947692
version: 1
top level: U061O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947692/range1947692/1

https://synbiohub.org/public/igem/BBa_I732404/annotation1947692/range1947692/1
type: Range
persistent identity: range1947692
display id: range1947692
version: 1
direction: direction/reverse
top level: U061O11+D0
start: 288
end: 299
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1947700
display id: annotation1947700
version: 1
top level: U061O11+D0
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947700/range1947700/1

https://synbiohub.org/public/igem/BBa_I732404/annotation1947700/range1947700/1
type: Range
persistent identity: range1947700
display id: range1947700
version: 1
direction: direction/forward
top level: U061O11+D0
start: 1194
end: 1194
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1947699
display id: annotation1947699
version: 1
top level: U061O11+D0
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947699/range1947699/1

https://synbiohub.org/public/igem/BBa_I732404/annotation1947699/range1947699/1
type: Range
persistent identity: range1947699
display id: range1947699
version: 1
direction: direction/forward
top level: U061O11+D0
start: 1188
end: 1201
orientation: inline

lacZ
type: SequenceAnnotation
persistent identity: annotation1947689
display id: annotation1947689
version: 1
top level: U061O11+D0
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947689/range1947689/1

https://synbiohub.org/public/igem/BBa_I732404/annotation1947689/range1947689/1
type: Range
persistent identity: range1947689
display id: range1947689
version: 1
direction: direction/forward
top level: U061O11+D0
start: 46
end: 279
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1947686
display id: annotation1947686
version: 1
top level: U061O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947686/range1947686/1

https://synbiohub.org/public/igem/BBa_I732404/annotation1947686/range1947686/1
type: Range
persistent identity: range1947686
display id: range1947686
version: 1
direction: direction/reverse
top level: U061O11+D0
start: 28
end: 39
orientation: inline

BBa_I732006
type: SequenceAnnotation
persistent identity: annotation1947688
display id: annotation1947688
version: 1
top level: U061O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947688/range1947688/1

https://synbiohub.org/public/igem/BBa_I732404/annotation1947688/range1947688/1
type: Range
persistent identity: range1947688
display id: range1947688
version: 1
direction: direction/reverse
top level: U061O11+D0
start: 46
end: 279
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation1947687
display id: annotation1947687
version: 1
top level: U061O11+D0
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947687/range1947687/1

https://synbiohub.org/public/igem/BBa_I732404/annotation1947687/range1947687/1
type: Range
persistent identity: range1947687
display id: range1947687
version: 1
direction: direction/forward
top level: U061O11+D0
start: 32
end: 35
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1947698
display id: annotation1947698
version: 1
top level: U061O11+D0
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947698/range1947698/1

https://synbiohub.org/public/igem/BBa_I732404/annotation1947698/range1947698/1
type: Range
persistent identity: range1947698
display id: range1947698
version: 1
direction: direction/forward
top level: U061O11+D0
start: 1168
end: 1187
orientation: inline

BglII
type: SequenceAnnotation
persistent identity: annotation1947684
display id: annotation1947684
version: 1
top level: U061O11+D0
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947684/range1947684/1

https://synbiohub.org/public/igem/BBa_I732404/annotation1947684/range1947684/1
type: Range
persistent identity: range1947684
display id: range1947684
version: 1
direction: direction/forward
top level: U061O11+D0
start: 3
end: 8
orientation: inline

BamHI
type: SequenceAnnotation
persistent identity: annotation1947685
display id: annotation1947685
version: 1
top level: U061O11+D0
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947685/range1947685/1

https://synbiohub.org/public/igem/BBa_I732404/annotation1947685/range1947685/1
type: Range
persistent identity: range1947685
display id: range1947685
version: 1
direction: direction/forward
top level: U061O11+D0
start: 12
end: 17
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation1947691
display id: annotation1947691
version: 1
top level: U061O11+D0
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947691/range1947691/1

https://synbiohub.org/public/igem/BBa_I732404/annotation1947691/range1947691/1
type: Range
persistent identity: range1947691
display id: range1947691
version: 1
direction: direction/forward
top level: U061O11+D0
start: 274
end: 279
orientation: inline

BBa_E0044
type: SequenceAnnotation
persistent identity: annotation1947694
display id: annotation1947694
version: 1
top level: U061O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947694/range1947694/1

https://synbiohub.org/public/igem/BBa_I732404/annotation1947694/range1947694/1
type: Range
persistent identity: range1947694
display id: range1947694
version: 1
direction: direction/reverse
top level: U061O11+D0
start: 306
end: 1064
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1947696
display id: annotation1947696
version: 1
top level: U061O11+D0
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947696/range1947696/1

https://synbiohub.org/public/igem/BBa_I732404/annotation1947696/range1947696/1
type: Range
persistent identity: range1947696
display id: range1947696
version: 1
direction: direction/forward
top level: U061O11+D0
start: 1084
end: 1127
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation1947693
display id: annotation1947693
version: 1
top level: U061O11+D0
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947693/range1947693/1

https://synbiohub.org/public/igem/BBa_I732404/annotation1947693/range1947693/1
type: Range
persistent identity: range1947693
display id: range1947693
version: 1
direction: direction/forward
top level: U061O11+D0
start: 292
end: 295
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1947697
display id: annotation1947697
version: 1
top level: U061O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947697/range1947697/1

https://synbiohub.org/public/igem/BBa_I732404/annotation1947697/range1947697/1
type: Range
persistent identity: range1947697
display id: range1947697
version: 1
direction: direction/forward
top level: U061O11+D0
start: 1161
end: 1201
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1947695
display id: annotation1947695
version: 1
top level: U061O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947695/range1947695/1

https://synbiohub.org/public/igem/BBa_I732404/annotation1947695/range1947695/1
type: Range
persistent identity: range1947695
display id: range1947695
version: 1
direction: direction/forward
top level: U061O11+D0
start: 1073
end: 1152
orientation: inline

BBa_J64804
type: ComponentDefinition
persistent identity: BBa J64804
display id: BBa_J64804
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: The promoter region (inclusive of regulator binding sites) of the B. subtilis RocDEF operon
creator: Sai Duriseti
created: 2007-03-19T12:00:00Z
modified: 2015-05-08T01:08:17Z
discontinued: false
dominant: false
group u list: _98_
m user id: 0
owner id: 1428
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the reported promoter region from the Bacillus subtilis RocDEF operon as taken from the DBTBS website (http://dbtbs.hgc.jp/). Base 1 to base 21 contains the binding site for the binding factor, RocR. The sequence, TATGCAAAAGAATTTTGCACT, reportedly contains the consensus sequence for binding of this factor. Base 42 to base 62 contains another binding site for the RocR binding factor, with ATATCAGAATGTTTTTGCACC as the binding consensus for this sequence. Bases 113-135 outline the binding site of the binding factor AhrC (CTTGCATTTATATAAAGGGAAAG). Bases 96 to 135 outline the promoter region for this operon (CTTGATTTGGCACAGAACTTGCATTTATATAAAGGGAAAG).
mutable notes: This sequence only outlines the reported promoter region as ascertained from the online resource described above, and may lack nucleotides not described by the operon analysis used.
mutable provenance: Information was obtained from: http://dbtbs.hgc.jp/COG/prom/rocDEF.html.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: AhrC
sequence annotation: RocR Binding Site
sequence annotation: Promoter
sequence: https://synbiohub.org/public/igem/BBa_J64804_sequence/1

AhrC
type: SequenceAnnotation
persistent identity: annotation1920955
display id: annotation1920955
version: 1
top level: BBa_J64804
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J64804/annotation1920955/range1920955/1

https://synbiohub.org/public/igem/BBa_J64804/annotation1920955/range1920955/1
type: Range
persistent identity: range1920955
display id: range1920955
version: 1
direction: direction/forward
top level: BBa_J64804
start: 113
end: 131
orientation: inline

RocR Binding Site
type: SequenceAnnotation
persistent identity: annotation1920951
display id: annotation1920951
version: 1
top level: BBa_J64804
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J64804/annotation1920951/range1920951/1

https://synbiohub.org/public/igem/BBa_J64804/annotation1920951/range1920951/1
type: Range
persistent identity: range1920951
display id: range1920951
version: 1
direction: direction/forward
top level: BBa_J64804
start: 1
end: 21
orientation: inline

RocR Binding Site
type: SequenceAnnotation
persistent identity: annotation1920952
display id: annotation1920952
version: 1
top level: BBa_J64804
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J64804/annotation1920952/range1920952/1

https://synbiohub.org/public/igem/BBa_J64804/annotation1920952/range1920952/1
type: Range
persistent identity: range1920952
display id: range1920952
version: 1
direction: direction/forward
top level: BBa_J64804
start: 42
end: 62
orientation: inline

Promoter
type: SequenceAnnotation
persistent identity: annotation1920953
display id: annotation1920953
version: 1
top level: BBa_J64804
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J64804/annotation1920953/range1920953/1

https://synbiohub.org/public/igem/BBa_J64804/annotation1920953/range1920953/1
type: Range
persistent identity: range1920953
display id: range1920953
version: 1
direction: direction/forward
top level: BBa_J64804
start: 96
end: 135
orientation: inline

BBa_K091107
type: ComponentDefinition
persistent identity: BBa K091107
display id: BBa_K091107
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLux/cI Hybrid Promoter
creator: Xiao Zhu
created: 2008-06-18T11:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _191_
m user id: 0
owner id: 3130
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This hybrid promoter is designed to respond to activation by LuxR when it is bound to AHL and to repression by the phage lambda cI repressor. The repression needs to win over activation.
mutable notes: This part is based on the idea of replacing the region of the Lux promoter downstream of the -35 with the OR2 cI half-operator, added LuxR-10 region. Based on what we have found up to now, promoters which are constructed just by inserting OR1/2 downstream of Lux are all very leaky.
mutable provenance: this part comes from LuxR promoter and cI lambda promoter.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Lux -10
sequence annotation: Lux Box
sequence annotation: Lux -35
sequence annotation: cI OR2
sequence: https://synbiohub.org/public/igem/BBa_K091107_sequence/1

Lux -10
type: SequenceAnnotation
persistent identity: annotation1963753
display id: annotation1963753
version: 1
top level: BBa_K091107
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K091107/annotation1963753/range1963753/1

https://synbiohub.org/public/igem/BBa_K091107/annotation1963753/range1963753/1
type: Range
persistent identity: range1963753
display id: range1963753
version: 1
direction: direction/forward
top level: BBa_K091107
start: 44
end: 49
orientation: inline

Lux Box
type: SequenceAnnotation
persistent identity: annotation1963750
display id: annotation1963750
version: 1
top level: BBa_K091107
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K091107/annotation1963750/range1963750/1

https://synbiohub.org/public/igem/BBa_K091107/annotation1963750/range1963750/1
type: Range
persistent identity: range1963750
display id: range1963750
version: 1
direction: direction/forward
top level: BBa_K091107
start: 1
end: 20
orientation: inline

Lux -35
type: SequenceAnnotation
persistent identity: annotation1963751
display id: annotation1963751
version: 1
top level: BBa_K091107
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K091107/annotation1963751/range1963751/1

https://synbiohub.org/public/igem/BBa_K091107/annotation1963751/range1963751/1
type: Range
persistent identity: range1963751
display id: range1963751
version: 1
direction: direction/forward
top level: BBa_K091107
start: 20
end: 25
orientation: inline

cI OR2
type: SequenceAnnotation
persistent identity: annotation1963752
display id: annotation1963752
version: 1
top level: BBa_K091107
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K091107/annotation1963752/range1963752/1

https://synbiohub.org/public/igem/BBa_K091107/annotation1963752/range1963752/1
type: Range
persistent identity: range1963752
display id: range1963752
version: 1
direction: direction/forward
top level: BBa_K091107
start: 26
end: 43
orientation: inline

Lux
type: ComponentDefinition
persistent identity: BBa I1051
display id: BBa_I1051
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lux cassette right promoter
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:30Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux repressor, LuxR. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.In thisversion, a binding site for cI dimer has been added: binding to this site dominantly represses transcription.
mutable provenance: V. fishceri and phage lambda (courtesy of Ron Weiss)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: start
sequence annotation: BBa_I1051
sequence annotation: LuxR/HSL
sequence annotation: cI (OR1)
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_I1051_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation1937
display id: annotation1937
version: 1
top level: Lux
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I1051/annotation1937/range1937/1

https://synbiohub.org/public/igem/BBa_I1051/annotation1937/range1937/1
type: Range
persistent identity: range1937
display id: range1937
version: 1
direction: direction/forward
top level: Lux
start: 20
end: 25
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1940
display id: annotation1940
version: 1
top level: Lux
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I1051/annotation1940/range1940/1

https://synbiohub.org/public/igem/BBa_I1051/annotation1940/range1940/1
type: Range
persistent identity: range1940
display id: range1940
version: 1
direction: direction/forward
top level: Lux
start: 53
end: 53
orientation: inline

BBa_I1051
type: SequenceAnnotation
persistent identity: annotation7059
display id: annotation7059
version: 1
top level: Lux
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I1051/annotation7059/range7059/1

https://synbiohub.org/public/igem/BBa_I1051/annotation7059/range7059/1
type: Range
persistent identity: range7059
display id: range7059
version: 1
direction: direction/forward
top level: Lux
start: 1
end: 68
orientation: inline

LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation1936
display id: annotation1936
version: 1
top level: Lux
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I1051/annotation1936/range1936/1

https://synbiohub.org/public/igem/BBa_I1051/annotation1936/range1936/1
type: Range
persistent identity: range1936
display id: range1936
version: 1
direction: direction/forward
top level: Lux
start: 1
end: 20
orientation: inline

cI (OR1)
type: SequenceAnnotation
persistent identity: annotation1939
display id: annotation1939
version: 1
top level: Lux
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I1051/annotation1939/range1939/1

https://synbiohub.org/public/igem/BBa_I1051/annotation1939/range1939/1
type: Range
persistent identity: range1939
display id: range1939
version: 1
direction: direction/forward
top level: Lux
start: 52
end: 68
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1938
display id: annotation1938
version: 1
top level: Lux
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I1051/annotation1938/range1938/1

https://synbiohub.org/public/igem/BBa_I1051/annotation1938/range1938/1
type: Range
persistent identity: range1938
display id: range1938
version: 1
direction: direction/forward
top level: Lux
start: 42
end: 47
orientation: inline

U049O11+D0
type: ComponentDefinition
persistent identity: BBa I732439
display id: BBa_I732439
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U049O11+D026O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732439_sequence/1

BBa_I741019
type: ComponentDefinition
persistent identity: BBa I741019
display id: BBa_I741019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Right facing promoter (for xylA) controlled by xylR and CRP-cAMP
creator: Lucien Weiss
created: 2007-06-20T11:00:00Z
modified: 2015-08-31T04:08:02Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _143_
m user id: 0
owner id: 1763
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: naturally the promoter to xylA and xylB
mutable notes: removed RBS cut out several xylR activation sites
mutable provenance: ecoli k12 chromosome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: xylAp
sequence annotation: XylR-Xylose transcriptional activator activates transcription
sequence annotation: CRP-cAMP transcriptional dual regulator activates transcription
sequence: https://synbiohub.org/public/igem/BBa_I741019_sequence/1

xylAp
type: SequenceAnnotation
persistent identity: annotation1935191
display id: annotation1935191
version: 1
top level: BBa_I741019
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I741019/annotation1935191/range1935191/1

https://synbiohub.org/public/igem/BBa_I741019/annotation1935191/range1935191/1
type: Range
persistent identity: range1935191
display id: range1935191
version: 1
direction: direction/reverse
top level: BBa_I741019
start: 76
end: 117
orientation: inline

XylR-Xylose transcriptional activator activates transcription
type: SequenceAnnotation
persistent identity: annotation1935195
display id: annotation1935195
version: 1
top level: BBa_I741019
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I741019/annotation1935195/range1935195/1

https://synbiohub.org/public/igem/BBa_I741019/annotation1935195/range1935195/1
type: Range
persistent identity: range1935195
display id: range1935195
version: 1
direction: direction/forward
top level: BBa_I741019
start: 69
end: 85
orientation: inline

CRP-cAMP transcriptional dual regulator activates transcription
type: SequenceAnnotation
persistent identity: annotation1935193
display id: annotation1935193
version: 1
top level: BBa_I741019
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I741019/annotation1935193/range1935193/1

https://synbiohub.org/public/igem/BBa_I741019/annotation1935193/range1935193/1
type: Range
persistent identity: range1935193
display id: range1935193
version: 1
direction: direction/forward
top level: BBa_I741019
start: 86
end: 107
orientation: inline

XylR-Xylose transcriptional activator activates transcription
type: SequenceAnnotation
persistent identity: annotation1935211
display id: annotation1935211
version: 1
top level: BBa_I741019
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I741019/annotation1935211/range1935211/1

https://synbiohub.org/public/igem/BBa_I741019/annotation1935211/range1935211/1
type: Range
persistent identity: range1935211
display id: range1935211
version: 1
direction: direction/reverse
top level: BBa_I741019
start: 48
end: 64
orientation: inline

BBa_K1486008
type: ComponentDefinition
persistent identity: BBa K1486008
display id: BBa_K1486008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CxpR & Split IFP1.4 [Cterm + Cterm][1]
creator: iGem EPFL 2014
created: 2014-08-25T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part has two fused proteins downstream of an arabinose-inducible promoter: CpxR-IFP[1] and CpxR-IFP[2]. This construct was made in all orientations: - IFP[1]-CpxR + IFP[2]-CpxR (BBa_K1286009) - IFP[1]-CpxR + CpxR-IFP[2] (BBa_K1486010) - CpxR-IFP[1] + IFP[2]-CpxR (BBa_K1486011)
mutable notes: All orientations were made, so all dimerizations could be tested (see main page for biobrick numbers). Flexible linkers were used for each of the fused proteins, and an RBS was put before each one of them.
mutable provenance: From Michnick Article. To Edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1486004
component: BBa_K1486000
component: BBa_B0034
component: BBa_K1486007
component: BBa_K1486006
component: BBa_K1486052
component: BBa_I0500
sequence annotation: BBa_K1486052
sequence annotation: BBa_K1486007
sequence annotation: BBa_K1486000
sequence annotation: BBa_K1486006
sequence annotation: BBa_B0034
sequence annotation: BBa_K1486004
sequence annotation: BBa_I0500
sequence: https://synbiohub.org/public/igem/BBa_K1486008_sequence/1

BBa_K1486004
type: Component
persistent identity: component2418041
display id: component2418041
version: 1
top level: BBa_K1486008
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_K1486000
type: Component
persistent identity: component2418040
display id: component2418040
version: 1
top level: BBa_K1486008
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_B0034
type: Component
persistent identity: component2418036
display id: component2418036
version: 1
top level: BBa_K1486008
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486007
type: Component
persistent identity: component2418057
display id: component2418057
version: 1
top level: BBa_K1486008
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486007/1

BBa_B0034
type: Component
persistent identity: component2418048
display id: component2418048
version: 1
top level: BBa_K1486008
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486006
type: Component
persistent identity: component2418045
display id: component2418045
version: 1
top level: BBa_K1486008
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486006/1

BBa_K1486052
type: Component
persistent identity: component2418046
display id: component2418046
version: 1
top level: BBa_K1486008
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486052/1

BBa_K1486004
type: Component
persistent identity: component2418053
display id: component2418053
version: 1
top level: BBa_K1486008
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_K1486000
type: Component
persistent identity: component2418052
display id: component2418052
version: 1
top level: BBa_K1486008
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_I0500
type: Component
persistent identity: component2418034
display id: component2418034
version: 1
top level: BBa_K1486008
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_K1486052
type: SequenceAnnotation
persistent identity: annotation2418046
display id: annotation2418046
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418046/range2418046/1
component: BBa_K1486052

https://synbiohub.org/public/igem/BBa_K1486008/annotation2418046/range2418046/1
type: Range
persistent identity: range2418046
display id: range2418046
version: 1
direction: direction/reverse
top level: BBa_K1486008
start: 2360
end: 2397
orientation: inline

BBa_K1486007
type: SequenceAnnotation
persistent identity: annotation2418057
display id: annotation2418057
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418057/range2418057/1
component: BBa_K1486007

https://synbiohub.org/public/igem/BBa_K1486008/annotation2418057/range2418057/1
type: Range
persistent identity: range2418057
display id: range2418057
version: 1
direction: direction/reverse
top level: BBa_K1486008
start: 3142
end: 3726
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2418040
display id: annotation2418040
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418040/range2418040/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486008/annotation2418040/range2418040/1
type: Range
persistent identity: range2418040
display id: range2418040
version: 1
direction: direction/reverse
top level: BBa_K1486008
start: 1223
end: 1924
orientation: inline

BBa_K1486006
type: SequenceAnnotation
persistent identity: annotation2418045
display id: annotation2418045
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418045/range2418045/1
component: BBa_K1486006

https://synbiohub.org/public/igem/BBa_K1486008/annotation2418045/range2418045/1
type: Range
persistent identity: range2418045
display id: range2418045
version: 1
direction: direction/reverse
top level: BBa_K1486008
start: 1955
end: 2359
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2418036
display id: annotation2418036
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418036/range2418036/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486008/annotation2418036/range2418036/1
type: Range
persistent identity: range2418036
display id: range2418036
version: 1
direction: direction/reverse
top level: BBa_K1486008
start: 1211
end: 1222
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2418052
display id: annotation2418052
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418052/range2418052/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486008/annotation2418052/range2418052/1
type: Range
persistent identity: range2418052
display id: range2418052
version: 1
direction: direction/reverse
top level: BBa_K1486008
start: 2410
end: 3111
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2418053
display id: annotation2418053
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418053/range2418053/1
component: BBa_K1486004

https://synbiohub.org/public/igem/BBa_K1486008/annotation2418053/range2418053/1
type: Range
persistent identity: range2418053
display id: range2418053
version: 1
direction: direction/reverse
top level: BBa_K1486008
start: 3112
end: 3141
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2418041
display id: annotation2418041
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418041/range2418041/1
component: BBa_K1486004

https://synbiohub.org/public/igem/BBa_K1486008/annotation2418041/range2418041/1
type: Range
persistent identity: range2418041
display id: range2418041
version: 1
direction: direction/reverse
top level: BBa_K1486008
start: 1925
end: 1954
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2418048
display id: annotation2418048
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418048/range2418048/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486008/annotation2418048/range2418048/1
type: Range
persistent identity: range2418048
display id: range2418048
version: 1
direction: direction/reverse
top level: BBa_K1486008
start: 2398
end: 2409
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2418034
display id: annotation2418034
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418034/range2418034/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486008/annotation2418034/range2418034/1
type: Range
persistent identity: range2418034
display id: range2418034
version: 1
direction: direction/reverse
top level: BBa_K1486008
start: 1
end: 1210
orientation: inline

U049O11+D0
type: ComponentDefinition
persistent identity: BBa I732441
display id: BBa_I732441
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U049O11+D050O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732441_sequence/1

U061O11+D0
type: ComponentDefinition
persistent identity: BBa I732431
display id: BBa_I732431
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U061O11+D002O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732431_sequence/1

BBa_K101000
type: ComponentDefinition
persistent identity: BBa K101000
display id: BBa_K101000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Dual-Repressed Promoter for p22 mnt and TetR
creator: Bennett Swiniarski
created: 2008-07-06T11:00:00Z
modified: 2015-05-08T01:08:41Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _205_
m user id: 0
owner id: 3363
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is a promoter that is repressed by both p22 mnt and TetR. It can be used to control the response of any gene that follows this promoter. It will be repressed by the presence of either p22 mnt or TetR.
mutable notes: TetR usually binds to two operator sites, one located upstream from -35 and one located between -35 and -10. However, p22 mnt also has two operator sites, one between -35 and -10 and another downstream from -10. Since each had an operator site in between -35 and -10, we had to eliminate the TetR operator site in order to allow the promoter to work. TetR was picked because it has a stronger binding than p22 mnt, and so would be more able to use only one site to bind.
mutable provenance: This part was designed by combining sequences from parts C0040 (TetR promoter) and C0072 (p22 mnt promoter).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K101000_sequence/1

NOR1
type: ComponentDefinition
persistent identity: BBa I732351
display id: BBa_I732351
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NOR Candidate (U037O11D002O22)
creator: Zhan Jian
created: 2007-10-02T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: true
experience: experience/Issues
group u list: _156_
m user id: 0
owner id: 1592
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: U97NUL D001O16
mutable notes: N/A
mutable provenance: LacIq
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732351_sequence/1

U097NUL+D0
type: ComponentDefinition
persistent identity: BBa I732400
display id: BBa_I732400
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U097NUL+D062NUL)
creator: Zhan Jian
created: 2007-10-03T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1572
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: P_template1 serves as the truss for PCR cloning. By respectively applying 7 different primers upstream and downstream, that is, 49 pairs of primers in total, we have got 49 different promoters. Then we made them digsted and loaded into a vector that has already possessed a lacZ-alpha fragment. In this way, we can read from the color of the clones on the plate whether the inserted promoter works or not. By this means, We can try seven different positions for Operator A and seven for Operator B, thus constructing 7*7 kinds of promoters which have actually constituted a specific promoter family. After transformed into the four test plasmids (pCR00, pCR01, pCR10, pCR11) together with a downstream double-reporter system, we will get to know the solo-repression and co-repression effects of the two repressors on the specific promoter.
mutable notes: None.
mutable provenance: None.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732400_sequence/1

U097O11+D0
type: ComponentDefinition
persistent identity: BBa I732415
display id: BBa_I732415
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U097O11+D026O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732415_sequence/1

BBa_K112701
type: ComponentDefinition
persistent identity: BBa K112701
display id: BBa_K112701
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: hns promoter
creator: Bing Xia
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:18Z
discontinued: false
dominant: false
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Phns in BBb1 format. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable provenance: Construction of [<Phns>] Basic Part K112701 PCR dv001/dv006 on Phns from E. coli K12 MG1655 (~342 bp, gp = A) PCR dv005/dv003 on Phns from E. coli K12 MG1655 (225 bp, gp = B) PCR dv004/dv002 on Phns from E. coli K12 MG1655 (~149 bp, gp = C) --------------------------------------------------- PCR dv001/dv003 on A+B (355 bp, gp = D) --------------------------------------------------- PCR dv001/dv002 on C+D (697bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+697) Product is pBca1256-K112701 ----------------------------------------------- dv001 Forward Biobricking of [<Phns>] CgATAgaattcATGagatctGAAATATAGCTGTGCCATCAGCC dv002 Reverse Biobricking of [<Phns>] cagtcggatccGCACGAAGAGTACGGATG dv003 Removing the 2nd EcoRI site in [<Phns>] (R) GCCAGGAATGTAAGGgATTCAAAATTGTTC dv004 Removing the 2ND EcoRI site in [<Phns>] (F) GAACAATTTTGAATcCCTTACATTCCTGGC dv005 Removing the FIRST EcoRI site in [<Phns>] (F)CGCTTAATAGGGgATTCTCGTAAACAC dv006 Removing the FIRST EcoRI site in [<Phns>] (R)GTGTTTACGAGAATcCCCTATTAAGCG
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K112701_sequence/1

BBa_K259013
type: ComponentDefinition
persistent identity: BBa K259013
display id: BBa_K259013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AraC-RBS-GFP-Terminator
creator: Petros Mina
created: 2009-10-14T11:00:00Z
modified: 2015-05-08T01:11:42Z
discontinued: false
dominant: false
group u list: _357_
m user id: 0
owner id: 5318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: A part used to quantify the AraC-RBS construct K259007 created by the BCCS-Bristol iGEM'09 team.
mutable notes: RFC10 assembly
mutable provenance: This part was designed with the specified biobricks from the parts registry. AraC occurs naturally in E.coli. GFP occurs in jellyfish species, RBS designed by the Anderson family and Terminator by Resma Shetty.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0011
component: BBa_B0012
component: BBa_J61100
component: BBa_R0080
component: BBa_E0040
component: BBa_R0081
sequence annotation: BBa_J61100
sequence annotation: BBa_R0081
sequence annotation: BBa_R0080
sequence annotation: BBa_B0011
sequence annotation: BBa_B0012
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_K259013_sequence/1

BBa_B0011
type: Component
persistent identity: component2041451
display id: component2041451
version: 1
top level: BBa_K259013
access: public
definition: https://synbiohub.org/public/igem/BBa_B0011/1

BBa_B0012
type: Component
persistent identity: component2041447
display id: component2041447
version: 1
top level: BBa_K259013
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_J61100
type: Component
persistent identity: component2041444
display id: component2041444
version: 1
top level: BBa_K259013
access: public
definition: https://synbiohub.org/public/igem/BBa_J61100/1

BBa_R0080
type: Component
persistent identity: component2041443
display id: component2041443
version: 1
top level: BBa_K259013
access: public
definition: https://synbiohub.org/public/igem/BBa_R0080/1

BBa_E0040
type: Component
persistent identity: component2041446
display id: component2041446
version: 1
top level: BBa_K259013
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_R0081
type: Component
persistent identity: component2041436
display id: component2041436
version: 1
top level: BBa_K259013
access: public
definition: https://synbiohub.org/public/igem/BBa_R0081/1

BBa_J61100
type: SequenceAnnotation
persistent identity: annotation2041444
display id: annotation2041444
version: 1
top level: BBa_K259013
location: https://synbiohub.org/public/igem/BBa_K259013/annotation2041444/range2041444/1
component: BBa_J61100

https://synbiohub.org/public/igem/BBa_K259013/annotation2041444/range2041444/1
type: Range
persistent identity: range2041444
display id: range2041444
version: 1
direction: direction/reverse
top level: BBa_K259013
start: 349
end: 360
orientation: inline

BBa_R0081
type: SequenceAnnotation
persistent identity: annotation2041436
display id: annotation2041436
version: 1
top level: BBa_K259013
location: https://synbiohub.org/public/igem/BBa_K259013/annotation2041436/range2041436/1
component: BBa_R0081

https://synbiohub.org/public/igem/BBa_K259013/annotation2041436/range2041436/1
type: Range
persistent identity: range2041436
display id: range2041436
version: 1
direction: direction/reverse
top level: BBa_K259013
start: 1
end: 183
orientation: inline

BBa_R0080
type: SequenceAnnotation
persistent identity: annotation2041443
display id: annotation2041443
version: 1
top level: BBa_K259013
location: https://synbiohub.org/public/igem/BBa_K259013/annotation2041443/range2041443/1
component: BBa_R0080

https://synbiohub.org/public/igem/BBa_K259013/annotation2041443/range2041443/1
type: Range
persistent identity: range2041443
display id: range2041443
version: 1
direction: direction/reverse
top level: BBa_K259013
start: 192
end: 340
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation2041451
display id: annotation2041451
version: 1
top level: BBa_K259013
location: https://synbiohub.org/public/igem/BBa_K259013/annotation2041451/range2041451/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_K259013/annotation2041451/range2041451/1
type: Range
persistent identity: range2041451
display id: range2041451
version: 1
direction: direction/forward
top level: BBa_K259013
start: 1144
end: 1189
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2041447
display id: annotation2041447
version: 1
top level: BBa_K259013
location: https://synbiohub.org/public/igem/BBa_K259013/annotation2041447/range2041447/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K259013/annotation2041447/range2041447/1
type: Range
persistent identity: range2041447
display id: range2041447
version: 1
direction: direction/forward
top level: BBa_K259013
start: 1095
end: 1135
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2041446
display id: annotation2041446
version: 1
top level: BBa_K259013
location: https://synbiohub.org/public/igem/BBa_K259013/annotation2041446/range2041446/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K259013/annotation2041446/range2041446/1
type: Range
persistent identity: range2041446
display id: range2041446
version: 1
direction: direction/reverse
top level: BBa_K259013
start: 367
end: 1086
orientation: inline

U085O11+D0
type: ComponentDefinition
persistent identity: BBa I732419
display id: BBa_I732419
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U085O11+D002O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732419_sequence/1

U061O11+D0
type: ComponentDefinition
persistent identity: BBa I732436
display id: BBa_I732436
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U061O11+D062O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732436_sequence/1

BBa_K136004
type: ComponentDefinition
persistent identity: BBa K136004
display id: BBa_K136004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter of flhDC gene from E. coli flagella
creator: Cyprien Maisonnier
created: 2008-08-11T11:00:00Z
modified: 2015-05-08T01:10:02Z
discontinued: true
dominant: false
experience: experience/None
group u list: _211_
m user id: 0
owner id: 3012
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: ''flhDC'' is the only class 1 gene that is part of the construction of the flagella. It is the master regulator of the biosynthesis of the flagella ( ''Ordering Genes in a Flagella Pathway by Analysis of Expression Kinetics from Living Bacteria'' - S. Kalir, J. McClure, K. Pabbaraju, C. Southward, M. Ronen, S. Leibler, M. G. Surette, U. Alon ). The promoter of ''flhDC'' is regulated by many transcription factors, for instance, OmpR* and EnvZ* are two repressors of this gene. '''Work in progress :''' Characterization of the repression by OmpR* and EnvZ*
mutable notes: The PCR on colony to amplify the promoter of ''flhDC'' did not work. We amplified both the promoter and the gene and then we did a specific amplification of the promoter from the full operon. We had a PCR amplicon of the right length this time. The primers used to amplify the promoter and the gene are : * Forward : GTTTCTTCGAATTCGCGGCCGCTTCTAGAGTTGTATGTGCGTGTAGTGACGAGTACAG * Reverse : GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAAACAGCCTGTACTCTCTGTTCATCC Then to amplify only the promoter we used : * Forward : GTTTCTTCGAATTCGCGGCCGCTTCTAGAGTTGTATGTGCGTGTAGTGACGAGTACAG * Reverse : GTTTCTTCCTGCAGCGGCCGCTACTAGTATCCCACCCAGAATAACCAACTTTAT
mutable provenance: This part comes from the genome of ''E.coli'' K12 strain MG1655.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OmpR binding site
sequence annotation: Transcription start base
sequence: https://synbiohub.org/public/igem/BBa_K136004_sequence/1

OmpR binding site
type: SequenceAnnotation
persistent identity: annotation1971578
display id: annotation1971578
version: 1
top level: BBa_K136004
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K136004/annotation1971578/range1971578/1

https://synbiohub.org/public/igem/BBa_K136004/annotation1971578/range1971578/1
type: Range
persistent identity: range1971578
display id: range1971578
version: 1
direction: direction/forward
top level: BBa_K136004
start: 43
end: 63
orientation: inline

Transcription start base
type: SequenceAnnotation
persistent identity: annotation1971577
display id: annotation1971577
version: 1
top level: BBa_K136004
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K136004/annotation1971577/range1971577/1

https://synbiohub.org/public/igem/BBa_K136004/annotation1971577/range1971577/1
type: Range
persistent identity: range1971577
display id: range1971577
version: 1
direction: direction/forward
top level: BBa_K136004
start: 36
end: 36
orientation: inline

BBa_I739102
type: ComponentDefinition
persistent identity: BBa I739102
display id: BBa_I739102
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Double Promoter (cI, negative / TetR, negative)
creator: Stefan Luzi
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:08:01Z
discontinued: false
dominant: false
group u list: _124_
m user id: 0
owner id: 2183
owning group id: 62
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: asdf
mutable notes: asdf
mutable provenance: Synthetic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: R0040 (2x TetR 1)
sequence annotation: R0051
sequence annotation: TetR 1
sequence annotation: Scar
sequence: https://synbiohub.org/public/igem/BBa_I739102_sequence/1

R0040 (2x TetR 1)
type: SequenceAnnotation
persistent identity: annotation1946933
display id: annotation1946933
version: 1
top level: BBa_I739102
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I739102/annotation1946933/range1946933/1

https://synbiohub.org/public/igem/BBa_I739102/annotation1946933/range1946933/1
type: Range
persistent identity: range1946933
display id: range1946933
version: 1
direction: direction/forward
top level: BBa_I739102
start: 58
end: 97
orientation: inline

R0051
type: SequenceAnnotation
persistent identity: annotation1946930
display id: annotation1946930
version: 1
top level: BBa_I739102
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I739102/annotation1946930/range1946930/1

https://synbiohub.org/public/igem/BBa_I739102/annotation1946930/range1946930/1
type: Range
persistent identity: range1946930
display id: range1946930
version: 1
direction: direction/forward
top level: BBa_I739102
start: 1
end: 49
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1946934
display id: annotation1946934
version: 1
top level: BBa_I739102
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I739102/annotation1946934/range1946934/1

https://synbiohub.org/public/igem/BBa_I739102/annotation1946934/range1946934/1
type: Range
persistent identity: range1946934
display id: range1946934
version: 1
direction: direction/forward
top level: BBa_I739102
start: 58
end: 77
orientation: inline

Scar
type: SequenceAnnotation
persistent identity: annotation1946931
display id: annotation1946931
version: 1
top level: BBa_I739102
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I739102/annotation1946931/range1946931/1

https://synbiohub.org/public/igem/BBa_I739102/annotation1946931/range1946931/1
type: Range
persistent identity: range1946931
display id: range1946931
version: 1
direction: direction/forward
top level: BBa_I739102
start: 50
end: 57
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1946932
display id: annotation1946932
version: 1
top level: BBa_I739102
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I739102/annotation1946932/range1946932/1

https://synbiohub.org/public/igem/BBa_I739102/annotation1946932/range1946932/1
type: Range
persistent identity: range1946932
display id: range1946932
version: 1
direction: direction/forward
top level: BBa_I739102
start: 78
end: 97
orientation: inline

pRM
type: ComponentDefinition
persistent identity: BBa K566001
display id: BBa_K566001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pRM promoter from Lambda
creator: Daniel Rodriguez, Eduardo Almeyda, Porfirio Quintero
created: 2011-09-25T11:00:00Z
modified: 2015-05-08T01:12:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _734_
m user id: 0
owner id: 8650
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: pRM promoter from Lambda phage. It may be positive and negative regulated according to cI protein concentration. It needs OL region in order to achieve better negative regulation. It may be used to establish a biphasic switch which could permit activation and repression of genes transcribed from it.
mutable notes: Analysis of illegal restriction sites was performed, but it does not have them originally.
mutable provenance: It comes directly from genomic DNA of Lambda phage. It was extracted by PCR as recommended by the Registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: pRM
sequence: https://synbiohub.org/public/igem/BBa_K566001_sequence/1

pRM
type: SequenceAnnotation
persistent identity: annotation2145891
display id: annotation2145891
version: 1
top level: pRM
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K566001/annotation2145891/range2145891/1

https://synbiohub.org/public/igem/BBa_K566001/annotation2145891/range2145891/1
type: Range
persistent identity: range2145891
display id: range2145891
version: 1
direction: direction/forward
top level: pRM
start: 1
end: 85
orientation: inline

Plac(rev)
type: ComponentDefinition
persistent identity: BBa I742124
display id: BBa_I742124
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Reverse complement Lac promoter
creator: Caroline Dahl
created: 2007-10-21T11:00:00Z
modified: 2015-08-31T04:08:02Z
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 1968
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is an 'upside-down' version of I14032. It is a strong, constitual promoter. By adding this part, we wish to emphasise the possibility of engineering both DNA strands, and the additional reglatory possibility that this gives. The upstream sequence is non-functional and simply there to faciliate gel analysis or to physically enable the part to be recomineered (see the Edinburgh 2007 project where it is flanked by dif-sites).
mutable notes: When dealing with reverse complements of a locus, suffix and prefix need be fitted in opposite order.
mutable provenance: This sequence is part of the E.coli K12 genome.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Plac -35
sequence annotation: LacI binding site
sequence annotation: Plac -10
sequence annotation: CAP binding site
sequence: https://synbiohub.org/public/igem/BBa_I742124_sequence/1

Plac -35
type: SequenceAnnotation
persistent identity: annotation1955298
display id: annotation1955298
version: 1
top level: Plac(rev)
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I742124/annotation1955298/range1955298/1

https://synbiohub.org/public/igem/BBa_I742124/annotation1955298/range1955298/1
type: Range
persistent identity: range1955298
display id: range1955298
version: 1
direction: direction/reverse
top level: Plac(rev)
start: 61
end: 66
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1955267
display id: annotation1955267
version: 1
top level: Plac(rev)
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I742124/annotation1955267/range1955267/1

https://synbiohub.org/public/igem/BBa_I742124/annotation1955267/range1955267/1
type: Range
persistent identity: range1955267
display id: range1955267
version: 1
direction: direction/reverse
top level: Plac(rev)
start: 11
end: 31
orientation: inline

Plac -10
type: SequenceAnnotation
persistent identity: annotation1955299
display id: annotation1955299
version: 1
top level: Plac(rev)
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I742124/annotation1955299/range1955299/1

https://synbiohub.org/public/igem/BBa_I742124/annotation1955299/range1955299/1
type: Range
persistent identity: range1955299
display id: range1955299
version: 1
direction: direction/reverse
top level: Plac(rev)
start: 38
end: 43
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1955266
display id: annotation1955266
version: 1
top level: Plac(rev)
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I742124/annotation1955266/range1955266/1

https://synbiohub.org/public/igem/BBa_I742124/annotation1955266/range1955266/1
type: Range
persistent identity: range1955266
display id: range1955266
version: 1
direction: direction/reverse
top level: Plac(rev)
start: 78
end: 115
orientation: inline

BBa_K109200
type: ComponentDefinition
persistent identity: BBa K109200
display id: BBa_K109200
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AraC and TetR promoter (hybrid)
creator: Faifan Tantakitti
created: 2008-07-27T11:00:00Z
modified: 2015-05-08T01:09:07Z
discontinued: false
dominant: false
experience: experience/None
group u list: _250_
m user id: 0
owner id: 3345
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is lacI. trial version.
mutable notes: ......blank2....
mutable provenance: .....blank1...
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: TetR
sequence annotation: -10
sequence annotation: -35
sequence annotation: AraC
sequence: https://synbiohub.org/public/igem/BBa_K109200_sequence/1

TetR
type: SequenceAnnotation
persistent identity: annotation1969066
display id: annotation1969066
version: 1
top level: BBa_K109200
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K109200/annotation1969066/range1969066/1

https://synbiohub.org/public/igem/BBa_K109200/annotation1969066/range1969066/1
type: Range
persistent identity: range1969066
display id: range1969066
version: 1
direction: direction/forward
top level: BBa_K109200
start: 81
end: 99
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1980471
display id: annotation1980471
version: 1
top level: BBa_K109200
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K109200/annotation1980471/range1980471/1

https://synbiohub.org/public/igem/BBa_K109200/annotation1980471/range1980471/1
type: Range
persistent identity: range1980471
display id: range1980471
version: 1
direction: direction/forward
top level: BBa_K109200
start: 73
end: 78
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1980470
display id: annotation1980470
version: 1
top level: BBa_K109200
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K109200/annotation1980470/range1980470/1

https://synbiohub.org/public/igem/BBa_K109200/annotation1980470/range1980470/1
type: Range
persistent identity: range1980470
display id: range1980470
version: 1
direction: direction/forward
top level: BBa_K109200
start: 49
end: 54
orientation: inline

AraC
type: SequenceAnnotation
persistent identity: annotation1969061
display id: annotation1969061
version: 1
top level: BBa_K109200
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K109200/annotation1969061/range1969061/1

https://synbiohub.org/public/igem/BBa_K109200/annotation1969061/range1969061/1
type: Range
persistent identity: range1969061
display id: range1969061
version: 1
direction: direction/forward
top level: BBa_K109200
start: 34
end: 50
orientation: inline

BBa_I739104
type: ComponentDefinition
persistent identity: BBa I739104
display id: BBa_I739104
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Double Promoter (LuxR/HSL, positive / P22 cII, negative)
creator: Stefan Luzi
created: 2007-10-14T11:00:00Z
modified: 2015-08-31T04:08:01Z
discontinued: false
dominant: false
group u list: _124_
m user id: 0
owner id: 2183
owning group id: 62
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: asdf
mutable notes: asdf
mutable provenance: Synthetic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OR1
sequence annotation: R0053 (2x OR1)
sequence annotation: Scar
sequence annotation: R0062
sequence: https://synbiohub.org/public/igem/BBa_I739104_sequence/1

OR1
type: SequenceAnnotation
persistent identity: annotation1947894
display id: annotation1947894
version: 1
top level: BBa_I739104
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I739104/annotation1947894/range1947894/1

https://synbiohub.org/public/igem/BBa_I739104/annotation1947894/range1947894/1
type: Range
persistent identity: range1947894
display id: range1947894
version: 1
direction: direction/forward
top level: BBa_I739104
start: 83
end: 101
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation1947893
display id: annotation1947893
version: 1
top level: BBa_I739104
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I739104/annotation1947893/range1947893/1

https://synbiohub.org/public/igem/BBa_I739104/annotation1947893/range1947893/1
type: Range
persistent identity: range1947893
display id: range1947893
version: 1
direction: direction/forward
top level: BBa_I739104
start: 64
end: 82
orientation: inline

R0053 (2x OR1)
type: SequenceAnnotation
persistent identity: annotation1947895
display id: annotation1947895
version: 1
top level: BBa_I739104
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I739104/annotation1947895/range1947895/1

https://synbiohub.org/public/igem/BBa_I739104/annotation1947895/range1947895/1
type: Range
persistent identity: range1947895
display id: range1947895
version: 1
direction: direction/forward
top level: BBa_I739104
start: 64
end: 101
orientation: inline

Scar
type: SequenceAnnotation
persistent identity: annotation1947892
display id: annotation1947892
version: 1
top level: BBa_I739104
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I739104/annotation1947892/range1947892/1

https://synbiohub.org/public/igem/BBa_I739104/annotation1947892/range1947892/1
type: Range
persistent identity: range1947892
display id: range1947892
version: 1
direction: direction/forward
top level: BBa_I739104
start: 56
end: 63
orientation: inline

R0062
type: SequenceAnnotation
persistent identity: annotation1947891
display id: annotation1947891
version: 1
top level: BBa_I739104
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I739104/annotation1947891/range1947891/1

https://synbiohub.org/public/igem/BBa_I739104/annotation1947891/range1947891/1
type: Range
persistent identity: range1947891
display id: range1947891
version: 1
direction: direction/forward
top level: BBa_I739104
start: 1
end: 55
orientation: inline

BBa_J05217
type: ComponentDefinition
persistent identity: BBa J05217
display id: BBa_J05217
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Regulator for R2-YAP7
creator: Alexander Roth
created: 2005-09-07T11:00:00Z
modified: 2015-08-31T04:08:15Z
discontinued: false
dominant: false
group u list: _34_
m user id: 0
owner id: 412
owning group id: 34
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: binding sites for R3-ATF6 and R4-cMaf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J05217_sequence/1

BBa_I751502
type: ComponentDefinition
persistent identity: BBa I751502
display id: BBa_I751502
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: plux-lac hybrid promoter
creator: Kenichiro Iwasaki
created: 2007-10-24T11:00:00Z
modified: 2015-08-31T04:08:06Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _151_
m user id: 0
owner id: 2188
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: sense TWO INPUTS, activation by AHL and repression by LacI.
mutable notes: Compared with existing BioBrick hybrid promoter R0065, this promoter is different in that its sequence is much shorter with lux box and -35 region overlapping, and that it is repressed by LacI, not cI. Also, the output is clearly detected as shown in Fig. 3.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: O1
sequence annotation: -10
sequence annotation: luxR binding site
sequence annotation: -35
sequence annotation: plac
sequence: https://synbiohub.org/public/igem/BBa_I751502_sequence/1

O1
type: SequenceAnnotation
persistent identity: annotation1955562
display id: annotation1955562
version: 1
top level: BBa_I751502
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I751502/annotation1955562/range1955562/1

https://synbiohub.org/public/igem/BBa_I751502/annotation1955562/range1955562/1
type: Range
persistent identity: range1955562
display id: range1955562
version: 1
direction: direction/forward
top level: BBa_I751502
start: 27
end: 41
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1955561
display id: annotation1955561
version: 1
top level: BBa_I751502
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I751502/annotation1955561/range1955561/1

https://synbiohub.org/public/igem/BBa_I751502/annotation1955561/range1955561/1
type: Range
persistent identity: range1955561
display id: range1955561
version: 1
direction: direction/forward
top level: BBa_I751502
start: 42
end: 48
orientation: inline

O1
type: SequenceAnnotation
persistent identity: annotation1955563
display id: annotation1955563
version: 1
top level: BBa_I751502
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I751502/annotation1955563/range1955563/1

https://synbiohub.org/public/igem/BBa_I751502/annotation1955563/range1955563/1
type: Range
persistent identity: range1955563
display id: range1955563
version: 1
direction: direction/forward
top level: BBa_I751502
start: 49
end: 74
orientation: inline

luxR binding site
type: SequenceAnnotation
persistent identity: annotation1955564
display id: annotation1955564
version: 1
top level: BBa_I751502
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I751502/annotation1955564/range1955564/1

https://synbiohub.org/public/igem/BBa_I751502/annotation1955564/range1955564/1
type: Range
persistent identity: range1955564
display id: range1955564
version: 1
direction: direction/forward
top level: BBa_I751502
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1955560
display id: annotation1955560
version: 1
top level: BBa_I751502
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I751502/annotation1955560/range1955560/1

https://synbiohub.org/public/igem/BBa_I751502/annotation1955560/range1955560/1
type: Range
persistent identity: range1955560
display id: range1955560
version: 1
direction: direction/forward
top level: BBa_I751502
start: 20
end: 26
orientation: inline

plac
type: SequenceAnnotation
persistent identity: annotation1955559
display id: annotation1955559
version: 1
top level: BBa_I751502
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I751502/annotation1955559/range1955559/1

https://synbiohub.org/public/igem/BBa_I751502/annotation1955559/range1955559/1
type: Range
persistent identity: range1955559
display id: range1955559
version: 1
direction: direction/forward
top level: BBa_I751502
start: 20
end: 74
orientation: inline