//proteindomain/transmembrane
type: Collection
persistent identity: proteindomain transmembrane
display id: proteindomain_transmembrane
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: EnvZ Tail
member: BBa_K1855005
member: BBa_K1936001
member: BBa_K1855003
member: BBa_K157002
member: BBa_K1855001
member: trkD
member: BBa_K1471000
member: BBa_K1909003
member: BBa_K1909006
member: BBa_K1855004
member: BBa_K2110003
member: BBa_K1855000
member: BBa_K1855002

BBa_K1936001
type: ComponentDefinition
persistent identity: BBa K1936001
display id: BBa_K1936001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tom5-eGFP-LOV2
creator: Marcel Dickmanns
created: 2016-10-12T11:00:00Z
modified: 2016-10-19T03:03:02Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2403_
m user id: 30478
owner id: 31678
owning group id: 9
status: status/Planning
bookmark: true
mutable description: ALERT !!!Under construction !!
mutable notes: ALERT !!!Under construction !!
mutable provenance: ALERT !!!Under construction !!
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence annotation: TOM5
sequence annotation: LOV2
sequence annotation: Linker
sequence annotation: eGFP
sequence: https://synbiohub.org/public/igem/BBa_K1936001_sequence/1

TOM5
type: SequenceAnnotation
persistent identity: annotation2521537
display id: annotation2521537
version: 1
top level: BBa_K1936001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1936001/annotation2521537/range2521537/1

https://synbiohub.org/public/igem/BBa_K1936001/annotation2521537/range2521537/1
type: Range
persistent identity: range2521537
display id: range2521537
version: 1
direction: direction/forward
top level: BBa_K1936001
start: 1
end: 153
orientation: inline

LOV2
type: SequenceAnnotation
persistent identity: annotation2521541
display id: annotation2521541
version: 1
top level: BBa_K1936001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1936001/annotation2521541/range2521541/1

https://synbiohub.org/public/igem/BBa_K1936001/annotation2521541/range2521541/1
type: Range
persistent identity: range2521541
display id: range2521541
version: 1
direction: direction/forward
top level: BBa_K1936001
start: 937
end: 1368
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2521540
display id: annotation2521540
version: 1
top level: BBa_K1936001
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1936001/annotation2521540/range2521540/1

https://synbiohub.org/public/igem/BBa_K1936001/annotation2521540/range2521540/1
type: Range
persistent identity: range2521540
display id: range2521540
version: 1
direction: direction/forward
top level: BBa_K1936001
start: 907
end: 936
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2521538
display id: annotation2521538
version: 1
top level: BBa_K1936001
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1936001/annotation2521538/range2521538/1

https://synbiohub.org/public/igem/BBa_K1936001/annotation2521538/range2521538/1
type: Range
persistent identity: range2521538
display id: range2521538
version: 1
direction: direction/forward
top level: BBa_K1936001
start: 154
end: 192
orientation: inline

eGFP
type: SequenceAnnotation
persistent identity: annotation2521539
display id: annotation2521539
version: 1
top level: BBa_K1936001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1936001/annotation2521539/range2521539/1

https://synbiohub.org/public/igem/BBa_K1936001/annotation2521539/range2521539/1
type: Range
persistent identity: range2521539
display id: range2521539
version: 1
direction: direction/forward
top level: BBa_K1936001
start: 193
end: 906
orientation: inline

BBa_K1909006
type: ComponentDefinition
persistent identity: BBa K1909006
display id: BBa_K1909006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: EnvZ-LRR chimeric receptor for mDAP recognition
creator: Daniel Wedemeyer
created: 2016-10-13T11:00:00Z
modified: 2016-10-14T11:35:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2375_
m user id: 0
m user id: 29525
owner id: 29525
owning group id: 9
sample status: Not in stock
status: status/Planning
status: status/Unavailable
bookmark: false
mutable description: coming soon
mutable notes: coming soon
mutable provenance: composite part
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000110
role: partType/Temporary
component: BBa_K1909005
component: BBa_K1909003
component: BBa_K1909004
sequence annotation: BBa_K1909005
sequence annotation: BBa_K1909003
sequence annotation: BBa_K1909004
sequence: https://synbiohub.org/public/igem/BBa_K1909006_sequence/1

BBa_K1909005
type: Component
persistent identity: component2507407
display id: component2507407
version: 1
top level: BBa_K1909006
access: public
definition: EnvZ Tail

BBa_K1909003
type: Component
persistent identity: component2507398
display id: component2507398
version: 1
top level: BBa_K1909006
access: public
definition: BBa_K1909003

BBa_K1909004
type: Component
persistent identity: component2507405
display id: component2507405
version: 1
top level: BBa_K1909006
access: public
definition: https://synbiohub.org/public/igem/BBa_K1909004/1

BBa_K1909005
type: SequenceAnnotation
persistent identity: annotation2507407
display id: annotation2507407
version: 1
top level: BBa_K1909006
location: https://synbiohub.org/public/igem/BBa_K1909006/annotation2507407/range2507407/1
component: BBa_K1909005

https://synbiohub.org/public/igem/BBa_K1909006/annotation2507407/range2507407/1
type: Range
persistent identity: range2507407
display id: range2507407
version: 1
direction: direction/reverse
top level: BBa_K1909006
start: 1143
end: 2024
orientation: inline

BBa_K1909003
type: SequenceAnnotation
persistent identity: annotation2507398
display id: annotation2507398
version: 1
top level: BBa_K1909006
location: https://synbiohub.org/public/igem/BBa_K1909006/annotation2507398/range2507398/1
component: BBa_K1909003

https://synbiohub.org/public/igem/BBa_K1909006/annotation2507398/range2507398/1
type: Range
persistent identity: range2507398
display id: range2507398
version: 1
direction: direction/reverse
top level: BBa_K1909006
start: 1
end: 161
orientation: inline

BBa_K1909004
type: SequenceAnnotation
persistent identity: annotation2507405
display id: annotation2507405
version: 1
top level: BBa_K1909006
location: https://synbiohub.org/public/igem/BBa_K1909006/annotation2507405/range2507405/1
component: BBa_K1909004

https://synbiohub.org/public/igem/BBa_K1909006/annotation2507405/range2507405/1
type: Range
persistent identity: range2507405
display id: range2507405
version: 1
direction: direction/reverse
top level: BBa_K1909006
start: 168
end: 1136
orientation: inline

BBa_K1855003
type: ComponentDefinition
persistent identity: BBa K1855003
display id: BBa_K1855003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lpp-OmpA sZF14
creator: Iain Galpin
created: 2015-09-16T11:00:00Z
modified: 2015-09-17T03:12:20Z
discontinued: false
dominant: false
group u list: _2282_
m user id: 27125
owner id: 27125
owning group id: 9
status: status/Planning
bookmark: false
mutable description: A fusion protein of a transmembrane domain (Lpp-OmpA) and a zinc-finger domain (sZF14),under pL_lac promotion and containing a C-terminal FLAG tag. It is used to exhibit a zinc-finger dsDNA binding domain complimentary to TCCTCACTC on the cell surface of E.coli.
mutable notes: We had to optimise the codon for E.coli K12, and then alter the codon slightly to remove unwanted cut sites. The Lpp-OmpA domain is preceeded by an NdeI cut site, and followed by an AgeI cut site. The sZF14 domain is preceeded by an NheI cut site and followed by a ClaI cut site.
mutable provenance: Lpp-OmpA is originally from E.coli; sZF2 is from http://www.nature.com/nmeth/journal/v10/n5/abs/nmeth.2407.html
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: pL_lac
sequence annotation: Linker
sequence annotation: Lpp-OmpA
sequence annotation: sZF14
sequence annotation: BBa_K864600 T0 Terminator
sequence annotation: 3x FLAG
sequence annotation: B0034 Strong RBS
sequence: https://synbiohub.org/public/igem/BBa_K1855003_sequence/1

pL_lac
type: SequenceAnnotation
persistent identity: annotation2466846
display id: annotation2466846
version: 1
top level: BBa_K1855003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1855003/annotation2466846/range2466846/1

https://synbiohub.org/public/igem/BBa_K1855003/annotation2466846/range2466846/1
type: Range
persistent identity: range2466846
display id: range2466846
version: 1
direction: direction/forward
top level: BBa_K1855003
start: 1
end: 56
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2466851
display id: annotation2466851
version: 1
top level: BBa_K1855003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1855003/annotation2466851/range2466851/1

https://synbiohub.org/public/igem/BBa_K1855003/annotation2466851/range2466851/1
type: Range
persistent identity: range2466851
display id: range2466851
version: 1
direction: direction/forward
top level: BBa_K1855003
start: 840
end: 863
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2466849
display id: annotation2466849
version: 1
top level: BBa_K1855003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1855003/annotation2466849/range2466849/1

https://synbiohub.org/public/igem/BBa_K1855003/annotation2466849/range2466849/1
type: Range
persistent identity: range2466849
display id: range2466849
version: 1
direction: direction/forward
top level: BBa_K1855003
start: 522
end: 557
orientation: inline

Lpp-OmpA
type: SequenceAnnotation
persistent identity: annotation2466848
display id: annotation2466848
version: 1
top level: BBa_K1855003
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1855003/annotation2466848/range2466848/1

https://synbiohub.org/public/igem/BBa_K1855003/annotation2466848/range2466848/1
type: Range
persistent identity: range2466848
display id: range2466848
version: 1
direction: direction/forward
top level: BBa_K1855003
start: 84
end: 518
orientation: inline

sZF14
type: SequenceAnnotation
persistent identity: annotation2466850
display id: annotation2466850
version: 1
top level: BBa_K1855003
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1855003/annotation2466850/range2466850/1

https://synbiohub.org/public/igem/BBa_K1855003/annotation2466850/range2466850/1
type: Range
persistent identity: range2466850
display id: range2466850
version: 1
direction: direction/forward
top level: BBa_K1855003
start: 564
end: 833
orientation: inline

BBa_K864600 T0 Terminator
type: SequenceAnnotation
persistent identity: annotation2466853
display id: annotation2466853
version: 1
top level: BBa_K1855003
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1855003/annotation2466853/range2466853/1

https://synbiohub.org/public/igem/BBa_K1855003/annotation2466853/range2466853/1
type: Range
persistent identity: range2466853
display id: range2466853
version: 1
direction: direction/forward
top level: BBa_K1855003
start: 945
end: 996
orientation: inline

3x FLAG
type: SequenceAnnotation
persistent identity: annotation2466852
display id: annotation2466852
version: 1
top level: BBa_K1855003
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1855003/annotation2466852/range2466852/1

https://synbiohub.org/public/igem/BBa_K1855003/annotation2466852/range2466852/1
type: Range
persistent identity: range2466852
display id: range2466852
version: 1
direction: direction/forward
top level: BBa_K1855003
start: 867
end: 938
orientation: inline

B0034 Strong RBS
type: SequenceAnnotation
persistent identity: annotation2466847
display id: annotation2466847
version: 1
top level: BBa_K1855003
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1855003/annotation2466847/range2466847/1

https://synbiohub.org/public/igem/BBa_K1855003/annotation2466847/range2466847/1
type: Range
persistent identity: range2466847
display id: range2466847
version: 1
direction: direction/forward
top level: BBa_K1855003
start: 60
end: 80
orientation: inline

EnvZ Tail
type: ComponentDefinition
persistent identity: BBa K1909005
display id: BBa_K1909005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: EnvZ Signal Transduction and Kinase Domains
creator: Daniel Wedemeyer
created: 2016-10-13T11:00:00Z
modified: 2016-10-14T11:30:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2375_
m user id: 29525
owner id: 29525
owning group id: 9
status: status/Planning
bookmark: false
mutable description: coming soon
mutable notes: coming soon
mutable provenance: E. coli EnvZ (ncbi: https://www.ncbi.nlm.nih.gov/gene/947272)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: EnvZ
sequence: https://synbiohub.org/public/igem/BBa_K1909005_sequence/1

EnvZ
type: SequenceAnnotation
persistent identity: annotation2507346
display id: annotation2507346
version: 1
top level: EnvZ Tail
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1909005/annotation2507346/range2507346/1

https://synbiohub.org/public/igem/BBa_K1909005/annotation2507346/range2507346/1
type: Range
persistent identity: range2507346
display id: range2507346
version: 1
direction: direction/forward
top level: EnvZ Tail
start: 1
end: 882
orientation: inline

BBa_K1855002
type: ComponentDefinition
persistent identity: BBa K1855002
display id: BBa_K1855002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lpp-OmpA sZF10
creator: Iain Galpin
created: 2015-09-16T11:00:00Z
modified: 2015-09-17T03:11:52Z
discontinued: false
dominant: false
group u list: _2282_
m user id: 27125
owner id: 27125
owning group id: 9
status: status/Planning
bookmark: false
mutable description: A fusion protein of a transmembrane domain (Lpp-OmpA) and a zinc-finger domain (sZF10),under pL_lac promotion and containing a C-terminal FLAG tag. It is used to exhibit a zinc-finger dsDNA binding domain complimentary to TGCTGCTTC on the cell surface of E.coli.
mutable notes: Lpp-OmpA is originally from E.coli; sZF10 is from http://www.nature.com/nmeth/journal/v10/n5/abs/nmeth.2407.html
mutable provenance: We had to optimise the codon for E.coli K12, and then alter the codon slightly to remove unwanted cut sites. The Lpp-OmpA domain is preceeded by an NdeI cut site, and followed by an AgeI cut site. The sZF10 domain is preceeded by an NheI cut site and followed by a ClaI cut site.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Linker
sequence annotation: BBa_K864600 T0 Terminator
sequence annotation: 3x FLAG
sequence annotation: Lpp-OmpA
sequence annotation: B0034 Strong RBS
sequence annotation: pL_lac
sequence annotation: sZF10
sequence: https://synbiohub.org/public/igem/BBa_K1855002_sequence/1

Linker
type: SequenceAnnotation
persistent identity: annotation2466781
display id: annotation2466781
version: 1
top level: BBa_K1855002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1855002/annotation2466781/range2466781/1

https://synbiohub.org/public/igem/BBa_K1855002/annotation2466781/range2466781/1
type: Range
persistent identity: range2466781
display id: range2466781
version: 1
direction: direction/forward
top level: BBa_K1855002
start: 522
end: 557
orientation: inline

BBa_K864600 T0 Terminator
type: SequenceAnnotation
persistent identity: annotation2466787
display id: annotation2466787
version: 1
top level: BBa_K1855002
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1855002/annotation2466787/range2466787/1

https://synbiohub.org/public/igem/BBa_K1855002/annotation2466787/range2466787/1
type: Range
persistent identity: range2466787
display id: range2466787
version: 1
direction: direction/forward
top level: BBa_K1855002
start: 945
end: 996
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2466784
display id: annotation2466784
version: 1
top level: BBa_K1855002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1855002/annotation2466784/range2466784/1

https://synbiohub.org/public/igem/BBa_K1855002/annotation2466784/range2466784/1
type: Range
persistent identity: range2466784
display id: range2466784
version: 1
direction: direction/forward
top level: BBa_K1855002
start: 840
end: 863
orientation: inline

3x FLAG
type: SequenceAnnotation
persistent identity: annotation2466785
display id: annotation2466785
version: 1
top level: BBa_K1855002
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1855002/annotation2466785/range2466785/1

https://synbiohub.org/public/igem/BBa_K1855002/annotation2466785/range2466785/1
type: Range
persistent identity: range2466785
display id: range2466785
version: 1
direction: direction/forward
top level: BBa_K1855002
start: 867
end: 938
orientation: inline

Lpp-OmpA
type: SequenceAnnotation
persistent identity: annotation2466779
display id: annotation2466779
version: 1
top level: BBa_K1855002
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1855002/annotation2466779/range2466779/1

https://synbiohub.org/public/igem/BBa_K1855002/annotation2466779/range2466779/1
type: Range
persistent identity: range2466779
display id: range2466779
version: 1
direction: direction/forward
top level: BBa_K1855002
start: 84
end: 518
orientation: inline

B0034 Strong RBS
type: SequenceAnnotation
persistent identity: annotation2466777
display id: annotation2466777
version: 1
top level: BBa_K1855002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1855002/annotation2466777/range2466777/1

https://synbiohub.org/public/igem/BBa_K1855002/annotation2466777/range2466777/1
type: Range
persistent identity: range2466777
display id: range2466777
version: 1
direction: direction/forward
top level: BBa_K1855002
start: 60
end: 80
orientation: inline

pL_lac
type: SequenceAnnotation
persistent identity: annotation2466776
display id: annotation2466776
version: 1
top level: BBa_K1855002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1855002/annotation2466776/range2466776/1

https://synbiohub.org/public/igem/BBa_K1855002/annotation2466776/range2466776/1
type: Range
persistent identity: range2466776
display id: range2466776
version: 1
direction: direction/forward
top level: BBa_K1855002
start: 1
end: 56
orientation: inline

sZF10
type: SequenceAnnotation
persistent identity: annotation2466782
display id: annotation2466782
version: 1
top level: BBa_K1855002
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1855002/annotation2466782/range2466782/1

https://synbiohub.org/public/igem/BBa_K1855002/annotation2466782/range2466782/1
type: Range
persistent identity: range2466782
display id: range2466782
version: 1
direction: direction/forward
top level: BBa_K1855002
start: 564
end: 833
orientation: inline

BBa_K1855000
type: ComponentDefinition
persistent identity: BBa K1855000
display id: BBa_K1855000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lpp-OmpA Zif268
creator: Iain Galpin
created: 2015-09-16T11:00:00Z
modified: 2015-09-17T03:10:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2282_
m user id: 27125
owner id: 27125
owning group id: 9
status: status/Planning
bookmark: false
mutable description: A fusion protein of a transmembrane domain (Lpp-OmpA) and a zinc-finger domain (Zif268 1AAY fragment),under pL_lac promotion and containing a C-terminal FLAG tag. It is used to exhibit a zinc-finger dsDNA binding domain complimentary to CCGCCCNCGC on the cell surface of E.coli.
mutable notes: We had to optimise the codon for E.coli K12, and then alter the codon slightly to remove unwanted cut sites.
mutable provenance: Lpp-OmpA is originally from E.coli; Zif268 is from mice.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Lpp-OmpA
sequence annotation: Zif268
sequence annotation: pL_lac
sequence annotation: B0034 Strong RBS
sequence annotation: 3x FLAG
sequence annotation: Linker
sequence annotation: BBa_K864600 T0 Terminator
sequence: https://synbiohub.org/public/igem/BBa_K1855000_sequence/1

Lpp-OmpA
type: SequenceAnnotation
persistent identity: annotation2466649
display id: annotation2466649
version: 1
top level: BBa_K1855000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1855000/annotation2466649/range2466649/1

https://synbiohub.org/public/igem/BBa_K1855000/annotation2466649/range2466649/1
type: Range
persistent identity: range2466649
display id: range2466649
version: 1
direction: direction/forward
top level: BBa_K1855000
start: 84
end: 518
orientation: inline

Zif268
type: SequenceAnnotation
persistent identity: annotation2466651
display id: annotation2466651
version: 1
top level: BBa_K1855000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1855000/annotation2466651/range2466651/1

https://synbiohub.org/public/igem/BBa_K1855000/annotation2466651/range2466651/1
type: Range
persistent identity: range2466651
display id: range2466651
version: 1
direction: direction/forward
top level: BBa_K1855000
start: 564
end: 830
orientation: inline

pL_lac
type: SequenceAnnotation
persistent identity: annotation2466646
display id: annotation2466646
version: 1
top level: BBa_K1855000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1855000/annotation2466646/range2466646/1

https://synbiohub.org/public/igem/BBa_K1855000/annotation2466646/range2466646/1
type: Range
persistent identity: range2466646
display id: range2466646
version: 1
direction: direction/forward
top level: BBa_K1855000
start: 1
end: 56
orientation: inline

B0034 Strong RBS
type: SequenceAnnotation
persistent identity: annotation2466647
display id: annotation2466647
version: 1
top level: BBa_K1855000
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1855000/annotation2466647/range2466647/1

https://synbiohub.org/public/igem/BBa_K1855000/annotation2466647/range2466647/1
type: Range
persistent identity: range2466647
display id: range2466647
version: 1
direction: direction/forward
top level: BBa_K1855000
start: 60
end: 80
orientation: inline

3x FLAG
type: SequenceAnnotation
persistent identity: annotation2466653
display id: annotation2466653
version: 1
top level: BBa_K1855000
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1855000/annotation2466653/range2466653/1

https://synbiohub.org/public/igem/BBa_K1855000/annotation2466653/range2466653/1
type: Range
persistent identity: range2466653
display id: range2466653
version: 1
direction: direction/forward
top level: BBa_K1855000
start: 864
end: 935
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2466650
display id: annotation2466650
version: 1
top level: BBa_K1855000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1855000/annotation2466650/range2466650/1

https://synbiohub.org/public/igem/BBa_K1855000/annotation2466650/range2466650/1
type: Range
persistent identity: range2466650
display id: range2466650
version: 1
direction: direction/forward
top level: BBa_K1855000
start: 522
end: 557
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2466652
display id: annotation2466652
version: 1
top level: BBa_K1855000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1855000/annotation2466652/range2466652/1

https://synbiohub.org/public/igem/BBa_K1855000/annotation2466652/range2466652/1
type: Range
persistent identity: range2466652
display id: range2466652
version: 1
direction: direction/forward
top level: BBa_K1855000
start: 837
end: 860
orientation: inline

BBa_K864600 T0 Terminator
type: SequenceAnnotation
persistent identity: annotation2466654
display id: annotation2466654
version: 1
top level: BBa_K1855000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1855000/annotation2466654/range2466654/1

https://synbiohub.org/public/igem/BBa_K1855000/annotation2466654/range2466654/1
type: Range
persistent identity: range2466654
display id: range2466654
version: 1
direction: direction/forward
top level: BBa_K1855000
start: 942
end: 993
orientation: inline

trkD
type: ComponentDefinition
persistent identity: BBa K629005
display id: BBa_K629005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: trkD, a functional Kup (formerly TrkD) system took up Cs+ with a moderate rate and affinity
creator: Zilong WANG, Yi ZHENG
created: 2011-10-03T11:00:00Z
modified: 2015-05-08T01:12:56Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _801_
m user id: 0
owner id: 9022
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Escherichia coli cells which contain a functional Kup (formerly TrkD) system took up Cs' with a moderate rate and affinity. Kup is a separate K+ uptake system with relatively little discrimination in the transport of the cations K+, Rb+, and Cs'. Regardless of the presence or absence of Kup, K+-replete cells took up Cs'primarily by a very low affinity mode, proportional to the ratio of the Cs' and K+ concentrations in the medium. In our project, trkD is used to collect Cs-137 when recN is activated by radiation and start the transcription of trkD,in order to absorb this kind of radioactive source like Cs-137
mutable notes: none
mutable provenance: ATGAGCACTGATAATAAGCAATCATTGCCCGCGATTACCCTCGCGGCGATTGGAGTTGTCTACGGCGATATTGGTACCAGCCCGTTATATACACTTCGTGAATGTTTGTCCGGCCAGTTTGGTTTTGGCGTTGAACGCGATGCCGTGTTTGGCTTTTTATCGCTGATCTTCTGGCTGCTAATCTTTGTGGTTTCCATTAAATATCTCACCTTCGTGATGCGGGCAGATAACGCCGGTGAGGGGGGGATCCTGACGTTGATGTCGCTTGCCGGGCGTAATACGTCGGCGCGAACCACATCAATGCTGGTGATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAGCATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCGATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGCTGTCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGACACTTTGGTAAGTTCCCTATTCGTCTGGCGTGGTTCACTGTCGTATTACCTTCCTTAACTCTTAATTACTTCGGCCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTTTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATCGCCTCGCAGGCGGTTATCTCTGGCGTCTTTTCATTGACGCGTCAGGCGGTACGTCTGGGATATTTGTCGCCGATGCGCATTATTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGGAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTGTGTCGATATTCCATTGTTCACCGCTAACCTCGATAAACTGCTCTCCGGCGGCTGGTTGCCATTGAGCCTCGGTACTGTGATGTTTATCGTGATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTGGAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGACCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTGTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: TrkD
sequence annotation: terminator
sequence annotation: start
sequence annotation: fwd primer
sequence annotation: rev primer
sequence: https://synbiohub.org/public/igem/BBa_K629005_sequence/1

TrkD
type: SequenceAnnotation
persistent identity: annotation2157756
display id: annotation2157756
version: 1
top level: trkD
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K629005/annotation2157756/range2157756/1

https://synbiohub.org/public/igem/BBa_K629005/annotation2157756/range2157756/1
type: Range
persistent identity: range2157756
display id: range2157756
version: 1
direction: direction/forward
top level: trkD
start: 1
end: 1869
orientation: inline

terminator
type: SequenceAnnotation
persistent identity: annotation2157758
display id: annotation2157758
version: 1
top level: trkD
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K629005/annotation2157758/range2157758/1

https://synbiohub.org/public/igem/BBa_K629005/annotation2157758/range2157758/1
type: Range
persistent identity: range2157758
display id: range2157758
version: 1
direction: direction/forward
top level: trkD
start: 1867
end: 1869
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2157754
display id: annotation2157754
version: 1
top level: trkD
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K629005/annotation2157754/range2157754/1

https://synbiohub.org/public/igem/BBa_K629005/annotation2157754/range2157754/1
type: Range
persistent identity: range2157754
display id: range2157754
version: 1
direction: direction/forward
top level: trkD
start: 1
end: 3
orientation: inline

fwd primer
type: SequenceAnnotation
persistent identity: annotation2157755
display id: annotation2157755
version: 1
top level: trkD
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K629005/annotation2157755/range2157755/1

https://synbiohub.org/public/igem/BBa_K629005/annotation2157755/range2157755/1
type: Range
persistent identity: range2157755
display id: range2157755
version: 1
direction: direction/forward
top level: trkD
start: 1
end: 21
orientation: inline

rev primer
type: SequenceAnnotation
persistent identity: annotation2157757
display id: annotation2157757
version: 1
top level: trkD
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K629005/annotation2157757/range2157757/1

https://synbiohub.org/public/igem/BBa_K629005/annotation2157757/range2157757/1
type: Range
persistent identity: range2157757
display id: range2157757
version: 1
direction: direction/reverse
top level: trkD
start: 1849
end: 1869
orientation: inline

BBa_K1471000
type: ComponentDefinition
persistent identity: BBa K1471000
display id: BBa_K1471000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MerE.
creator: Juan No?? Hern??ndez Salazar
created: 2014-10-07T11:00:00Z
modified: 2015-07-22T02:39:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1850_
m user id: 4206
owner id: 16464
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: For mercury bioaccumulation.
mutable notes: We have to optimizes its codons in Arabidopsis Thaliana and removed the restriction sites for EcoR1, Xba1, Spe1 and Pst1.
mutable provenance: Bacterial operon mer.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1471000_sequence/1

BBa_K2110003
type: ComponentDefinition
persistent identity: BBa K2110003
display id: BBa_K2110003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TPA transporting protein gene
creator: Le He
created: 2016-10-04T11:00:00Z
modified: 2016-10-06T12:50:35Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2578_
m user id: 32964
owner id: 32957
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This gene encodes the terephthalic acid(TPA) transporting protein. TPA is not a kind of nutrient substance for most organisms, including common chassis organisms like E.coli and Saccharomyces cerevisiae. However, as a kind of TPA degradable organism, RhodococcusjostiiRHA1 can transport TPA into their cells because of the TPA transporting protein genetpaK in their genome. This gene is also verified to be useful in Saccharomyces cerevisiae.
mutable notes: /
mutable provenance: It comes from the genome of RhodococcusjostiiRHA1.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: misc
sequence: https://synbiohub.org/public/igem/BBa_K2110003_sequence/1

misc
type: SequenceAnnotation
persistent identity: annotation2486172
display id: annotation2486172
version: 1
top level: BBa_K2110003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K2110003/annotation2486172/range2486172/1

https://synbiohub.org/public/igem/BBa_K2110003/annotation2486172/range2486172/1
type: Range
persistent identity: range2486172
display id: range2486172
version: 1
direction: direction/reverse
top level: BBa_K2110003
start: 1
end: 1380
orientation: inline

BBa_K1855005
type: ComponentDefinition
persistent identity: BBa K1855005
display id: BBa_K1855005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BclA-Zif268
creator: Iain Galpin
created: 2015-09-16T11:00:00Z
modified: 2015-09-17T03:13:15Z
discontinued: false
dominant: false
group u list: _2282_
m user id: 27125
owner id: 27125
owning group id: 9
status: status/Planning
bookmark: false
mutable description: A fusion protein of a transmembrane domain (BclA) and a zinc-finger domain (Zif268 1AAY fragment),under pL_lac promotion and containing a C-terminal FLAG tag. It is used to exhibit a zinc-finger dsDNA binding domain complimentary to CCGCCCNCGC on the cell surface of E.coli.
mutable notes: We had to optimise the codon for E.coli K12, and then alter the codon slightly to remove unwanted cut sites. The BclA domain is preceeded by an NdeI cut site, and followed by an AgeI cut site. The Zif268 fragment domain is preceeded by an NheI cut site and followed by a ClaI cut site.
mutable provenance: BclA is originally from Bacillus Anthracis; Zif268 is from mice.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Zif268
sequence annotation: Linker
sequence annotation: B0034 Strong RBS
sequence annotation: 3x FLAG
sequence annotation: pL_lac
sequence annotation: BBa_K864600 T0 Terminator
sequence annotation: BclA
sequence: https://synbiohub.org/public/igem/BBa_K1855005_sequence/1

Zif268
type: SequenceAnnotation
persistent identity: annotation2466913
display id: annotation2466913
version: 1
top level: BBa_K1855005
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1855005/annotation2466913/range2466913/1

https://synbiohub.org/public/igem/BBa_K1855005/annotation2466913/range2466913/1
type: Range
persistent identity: range2466913
display id: range2466913
version: 1
direction: direction/forward
top level: BBa_K1855005
start: 192
end: 458
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2466914
display id: annotation2466914
version: 1
top level: BBa_K1855005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1855005/annotation2466914/range2466914/1

https://synbiohub.org/public/igem/BBa_K1855005/annotation2466914/range2466914/1
type: Range
persistent identity: range2466914
display id: range2466914
version: 1
direction: direction/forward
top level: BBa_K1855005
start: 465
end: 488
orientation: inline

B0034 Strong RBS
type: SequenceAnnotation
persistent identity: annotation2466910
display id: annotation2466910
version: 1
top level: BBa_K1855005
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1855005/annotation2466910/range2466910/1

https://synbiohub.org/public/igem/BBa_K1855005/annotation2466910/range2466910/1
type: Range
persistent identity: range2466910
display id: range2466910
version: 1
direction: direction/forward
top level: BBa_K1855005
start: 60
end: 80
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2466912
display id: annotation2466912
version: 1
top level: BBa_K1855005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1855005/annotation2466912/range2466912/1

https://synbiohub.org/public/igem/BBa_K1855005/annotation2466912/range2466912/1
type: Range
persistent identity: range2466912
display id: range2466912
version: 1
direction: direction/forward
top level: BBa_K1855005
start: 150
end: 185
orientation: inline

3x FLAG
type: SequenceAnnotation
persistent identity: annotation2466915
display id: annotation2466915
version: 1
top level: BBa_K1855005
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1855005/annotation2466915/range2466915/1

https://synbiohub.org/public/igem/BBa_K1855005/annotation2466915/range2466915/1
type: Range
persistent identity: range2466915
display id: range2466915
version: 1
direction: direction/forward
top level: BBa_K1855005
start: 492
end: 563
orientation: inline

pL_lac
type: SequenceAnnotation
persistent identity: annotation2466909
display id: annotation2466909
version: 1
top level: BBa_K1855005
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1855005/annotation2466909/range2466909/1

https://synbiohub.org/public/igem/BBa_K1855005/annotation2466909/range2466909/1
type: Range
persistent identity: range2466909
display id: range2466909
version: 1
direction: direction/forward
top level: BBa_K1855005
start: 1
end: 56
orientation: inline

BBa_K864600 T0 Terminator
type: SequenceAnnotation
persistent identity: annotation2466916
display id: annotation2466916
version: 1
top level: BBa_K1855005
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1855005/annotation2466916/range2466916/1

https://synbiohub.org/public/igem/BBa_K1855005/annotation2466916/range2466916/1
type: Range
persistent identity: range2466916
display id: range2466916
version: 1
direction: direction/forward
top level: BBa_K1855005
start: 570
end: 621
orientation: inline

BclA
type: SequenceAnnotation
persistent identity: annotation2466911
display id: annotation2466911
version: 1
top level: BBa_K1855005
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1855005/annotation2466911/range2466911/1

https://synbiohub.org/public/igem/BBa_K1855005/annotation2466911/range2466911/1
type: Range
persistent identity: range2466911
display id: range2466911
version: 1
direction: direction/forward
top level: BBa_K1855005
start: 84
end: 146
orientation: inline

BBa_K1855001
type: ComponentDefinition
persistent identity: BBa K1855001
display id: BBa_K1855001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lpp-OmpA sZF2
creator: Iain Galpin
created: 2015-09-16T11:00:00Z
modified: 2015-09-17T03:11:22Z
discontinued: false
dominant: false
group u list: _2282_
m user id: 27125
owner id: 27125
owning group id: 9
status: status/Planning
bookmark: false
mutable description: A fusion protein of a transmembrane domain (Lpp-OmpA) and a zinc-finger domain (sZF2),under pL_lac promotion and containing a C-terminal FLAG tag. It is used to exhibit a zinc-finger dsDNA binding domain complimentary to TGCTGCTTC on the cell surface of E.coli.
mutable notes: We had to optimise the codon for E.coli K12, and then alter the codon slightly to remove unwanted cut sites. The Lpp-OmpA domain is preceeded by an NdeI cut site, and followed by an AgeI cut site. The sZF2 domain is preceeded by an NheI cut site and followed by a ClaI cut site.
mutable provenance: Lpp-OmpA is originally from E.coli; sZF2 is from http://www.nature.com/nmeth/journal/v10/n5/abs/nmeth.2407.html
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: sZF2
sequence annotation: B0034 Strong RBS
sequence annotation: pL_lac
sequence annotation: 3x FLAG
sequence annotation: Lpp-OmpA
sequence annotation: BBa_K864600 T0 Terminator
sequence annotation: Linker
sequence: https://synbiohub.org/public/igem/BBa_K1855001_sequence/1

sZF2
type: SequenceAnnotation
persistent identity: annotation2466711
display id: annotation2466711
version: 1
top level: BBa_K1855001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1855001/annotation2466711/range2466711/1

https://synbiohub.org/public/igem/BBa_K1855001/annotation2466711/range2466711/1
type: Range
persistent identity: range2466711
display id: range2466711
version: 1
direction: direction/forward
top level: BBa_K1855001
start: 564
end: 833
orientation: inline

B0034 Strong RBS
type: SequenceAnnotation
persistent identity: annotation2466694
display id: annotation2466694
version: 1
top level: BBa_K1855001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1855001/annotation2466694/range2466694/1

https://synbiohub.org/public/igem/BBa_K1855001/annotation2466694/range2466694/1
type: Range
persistent identity: range2466694
display id: range2466694
version: 1
direction: direction/forward
top level: BBa_K1855001
start: 60
end: 80
orientation: inline

pL_lac
type: SequenceAnnotation
persistent identity: annotation2466693
display id: annotation2466693
version: 1
top level: BBa_K1855001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1855001/annotation2466693/range2466693/1

https://synbiohub.org/public/igem/BBa_K1855001/annotation2466693/range2466693/1
type: Range
persistent identity: range2466693
display id: range2466693
version: 1
direction: direction/forward
top level: BBa_K1855001
start: 1
end: 56
orientation: inline

3x FLAG
type: SequenceAnnotation
persistent identity: annotation2466713
display id: annotation2466713
version: 1
top level: BBa_K1855001
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1855001/annotation2466713/range2466713/1

https://synbiohub.org/public/igem/BBa_K1855001/annotation2466713/range2466713/1
type: Range
persistent identity: range2466713
display id: range2466713
version: 1
direction: direction/forward
top level: BBa_K1855001
start: 867
end: 938
orientation: inline

Lpp-OmpA
type: SequenceAnnotation
persistent identity: annotation2466695
display id: annotation2466695
version: 1
top level: BBa_K1855001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1855001/annotation2466695/range2466695/1

https://synbiohub.org/public/igem/BBa_K1855001/annotation2466695/range2466695/1
type: Range
persistent identity: range2466695
display id: range2466695
version: 1
direction: direction/forward
top level: BBa_K1855001
start: 84
end: 518
orientation: inline

BBa_K864600 T0 Terminator
type: SequenceAnnotation
persistent identity: annotation2466714
display id: annotation2466714
version: 1
top level: BBa_K1855001
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1855001/annotation2466714/range2466714/1

https://synbiohub.org/public/igem/BBa_K1855001/annotation2466714/range2466714/1
type: Range
persistent identity: range2466714
display id: range2466714
version: 1
direction: direction/forward
top level: BBa_K1855001
start: 945
end: 996
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2466712
display id: annotation2466712
version: 1
top level: BBa_K1855001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1855001/annotation2466712/range2466712/1

https://synbiohub.org/public/igem/BBa_K1855001/annotation2466712/range2466712/1
type: Range
persistent identity: range2466712
display id: range2466712
version: 1
direction: direction/forward
top level: BBa_K1855001
start: 840
end: 863
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2466710
display id: annotation2466710
version: 1
top level: BBa_K1855001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1855001/annotation2466710/range2466710/1

https://synbiohub.org/public/igem/BBa_K1855001/annotation2466710/range2466710/1
type: Range
persistent identity: range2466710
display id: range2466710
version: 1
direction: direction/forward
top level: BBa_K1855001
start: 522
end: 557
orientation: inline

BBa_K1855004
type: ComponentDefinition
persistent identity: BBa K1855004
display id: BBa_K1855004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: INP-Zif268
creator: Iain Galpin
created: 2015-09-16T11:00:00Z
modified: 2015-09-17T03:12:50Z
discontinued: false
dominant: false
group u list: _2282_
m user id: 27125
owner id: 27125
owning group id: 9
status: status/Planning
bookmark: false
mutable description: A fusion protein of a transmembrane domain (INP) and a zinc-finger domain (Zif268 1AAY fragment),under pL_lac promotion and containing a C-terminal FLAG tag. It is used to exhibit a zinc-finger dsDNA binding domain complimentary to CCGCCCNCGC on the cell surface of E.coli.
mutable notes: We had to optimise the codon for E.coli K12, and then alter the codon slightly to remove unwanted cut sites. The INP domain is preceeded by an NdeI cut site, and followed by an AgeI cut site. The Zif268 fragment domain is preceeded by an NheI cut site and followed by a ClaI cut site.
mutable provenance: Lpp-OmpA is originally from Pseudomonas syringae; Zif268 is from mice.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Linker
sequence annotation: pL_lac
sequence annotation: INP
sequence annotation: Zif268
sequence annotation: 3x FLAG
sequence annotation: BBa_K864600 T0 Terminator
sequence annotation: B0034 Strong RBS
sequence: https://synbiohub.org/public/igem/BBa_K1855004_sequence/1

Linker
type: SequenceAnnotation
persistent identity: annotation2466889
display id: annotation2466889
version: 1
top level: BBa_K1855004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1855004/annotation2466889/range2466889/1

https://synbiohub.org/public/igem/BBa_K1855004/annotation2466889/range2466889/1
type: Range
persistent identity: range2466889
display id: range2466889
version: 1
direction: direction/forward
top level: BBa_K1855004
start: 1326
end: 1349
orientation: inline

pL_lac
type: SequenceAnnotation
persistent identity: annotation2466884
display id: annotation2466884
version: 1
top level: BBa_K1855004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1855004/annotation2466884/range2466884/1

https://synbiohub.org/public/igem/BBa_K1855004/annotation2466884/range2466884/1
type: Range
persistent identity: range2466884
display id: range2466884
version: 1
direction: direction/forward
top level: BBa_K1855004
start: 1
end: 56
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2466887
display id: annotation2466887
version: 1
top level: BBa_K1855004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1855004/annotation2466887/range2466887/1

https://synbiohub.org/public/igem/BBa_K1855004/annotation2466887/range2466887/1
type: Range
persistent identity: range2466887
display id: range2466887
version: 1
direction: direction/forward
top level: BBa_K1855004
start: 1011
end: 1046
orientation: inline

INP
type: SequenceAnnotation
persistent identity: annotation2466886
display id: annotation2466886
version: 1
top level: BBa_K1855004
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1855004/annotation2466886/range2466886/1

https://synbiohub.org/public/igem/BBa_K1855004/annotation2466886/range2466886/1
type: Range
persistent identity: range2466886
display id: range2466886
version: 1
direction: direction/forward
top level: BBa_K1855004
start: 84
end: 1007
orientation: inline

Zif268
type: SequenceAnnotation
persistent identity: annotation2466888
display id: annotation2466888
version: 1
top level: BBa_K1855004
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1855004/annotation2466888/range2466888/1

https://synbiohub.org/public/igem/BBa_K1855004/annotation2466888/range2466888/1
type: Range
persistent identity: range2466888
display id: range2466888
version: 1
direction: direction/forward
top level: BBa_K1855004
start: 1053
end: 1319
orientation: inline

3x FLAG
type: SequenceAnnotation
persistent identity: annotation2466890
display id: annotation2466890
version: 1
top level: BBa_K1855004
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1855004/annotation2466890/range2466890/1

https://synbiohub.org/public/igem/BBa_K1855004/annotation2466890/range2466890/1
type: Range
persistent identity: range2466890
display id: range2466890
version: 1
direction: direction/forward
top level: BBa_K1855004
start: 1353
end: 1424
orientation: inline

BBa_K864600 T0 Terminator
type: SequenceAnnotation
persistent identity: annotation2466891
display id: annotation2466891
version: 1
top level: BBa_K1855004
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1855004/annotation2466891/range2466891/1

https://synbiohub.org/public/igem/BBa_K1855004/annotation2466891/range2466891/1
type: Range
persistent identity: range2466891
display id: range2466891
version: 1
direction: direction/forward
top level: BBa_K1855004
start: 1431
end: 1482
orientation: inline

B0034 Strong RBS
type: SequenceAnnotation
persistent identity: annotation2466885
display id: annotation2466885
version: 1
top level: BBa_K1855004
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1855004/annotation2466885/range2466885/1

https://synbiohub.org/public/igem/BBa_K1855004/annotation2466885/range2466885/1
type: Range
persistent identity: range2466885
display id: range2466885
version: 1
direction: direction/forward
top level: BBa_K1855004
start: 60
end: 80
orientation: inline

BBa_K1909003
type: ComponentDefinition
persistent identity: BBa K1909003
display id: BBa_K1909003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: EnvZ Signal Peptide and first Transmembrane Domain
creator: Daniel Wedemeyer
created: 2016-10-13T11:00:00Z
modified: 2016-10-14T11:06:33Z
discontinued: false
dominant: false
group u list: _2375_
m user id: 29525
owner id: 29525
owning group id: 9
status: status/Planning
bookmark: false
mutable description: For EnvZ fusion protein design. Long description will be added soon.
mutable notes: coming soon
mutable provenance: Source: E. coli EnvZ protein (ncbi: https://www.ncbi.nlm.nih.gov/gene/947272)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: EnvZ
sequence annotation: FLAG
sequence: https://synbiohub.org/public/igem/BBa_K1909003_sequence/1

EnvZ
type: SequenceAnnotation
persistent identity: annotation2507125
display id: annotation2507125
version: 1
top level: BBa_K1909003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1909003/annotation2507125/range2507125/1

https://synbiohub.org/public/igem/BBa_K1909003/annotation2507125/range2507125/1
type: Range
persistent identity: range2507125
display id: range2507125
version: 1
direction: direction/forward
top level: BBa_K1909003
start: 1
end: 161
orientation: inline

FLAG
type: SequenceAnnotation
persistent identity: annotation2507149
display id: annotation2507149
version: 1
top level: BBa_K1909003
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1909003/annotation2507149/range2507149/1

https://synbiohub.org/public/igem/BBa_K1909003/annotation2507149/range2507149/1
type: Range
persistent identity: range2507149
display id: range2507149
version: 1
direction: direction/forward
top level: BBa_K1909003
start: 46
end: 69
orientation: inline

BBa_K157002
type: ComponentDefinition
persistent identity: BBa K157002
display id: BBa_K157002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Transmembrane region of the EGF-Receptor (ErbB-1)
creator: iGEM Team Freiburg 2008
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Helical, single-span transmembrane region of the human EGF-Receptor type ErbB-1, sequence taken from UniProtKB/Swiss-Prot entry P00533.
mutable notes: Between BioBrick 1.0 sites this part is flanked 5' by NgoMI(=NgoMIV) and 3' AgeI(=PinAI) to facilitate in frame cloning for protein fusions.
mutable provenance: Transmembrane region of the human EGF-Receptor type ErbB-1. Sequence taken from UniProtKB/Swiss-Prot entry P00533. Gene synthesis by ATG:biosynthetics, optimized for expression in homo sapiens by iGEM-Team Freiburg 2008.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: EGFR-Transmembrane region
sequence: https://synbiohub.org/public/igem/BBa_K157002_sequence/1

EGFR-Transmembrane region
type: SequenceAnnotation
persistent identity: annotation2064675
display id: annotation2064675
version: 1
top level: BBa_K157002
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157002/annotation2064675/range2064675/1

https://synbiohub.org/public/igem/BBa_K157002/annotation2064675/range2064675/1
type: Range
persistent identity: range2064675
display id: range2064675
version: 1
direction: direction/forward
top level: BBa_K157002
start: 1
end: 69
orientation: inline