//proteindomain/localization
type: Collection
persistent identity: proteindomain localization
display id: proteindomain_localization
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: CD4 signal
member: PKI NES
member: secretion
member: BBa_K801042
member: MFa1-Secre
member: BBa_K1680004
member: MTS
member: BBa_K1680013
member: BBa_K416008
member: BBa_K801039
member: BBa_K900002
member: slr2016 SS
member: BBa_K249005
member: GFP-PTS1
member: SV40 NLS
member: BBa_I712016
member: MLS
member: BBa_K1478000
member: BBa_K1909003
member: BBa_K801040
member: BBa_K801041
member: BBa_K283036
member: BBa_K392005
member: BBa_K1732000
member: BBa_K900005
member: eGFP-PTS1
member: BBa_K1642007
member: SicP-SptP
member: rbs.pelB
member: BBa_K1936001
member: BBa_K1642008
member: BBa_K900004
member: BBa_K416000
member: BBa_K416004
member: OmpA
member: BBa_K801043
member: pilA1 SS
member: PelB
member: BBa_K157010
member: BBa_K900003
member: BBa_K249004
member: BBa_K416003
member: BBa_K801030
member: mRFP1-NLS
member: myristoila
member: ErbB1 (SP)

BBa_K900003
type: ComponentDefinition
persistent identity: BBa K900003
display id: BBa_K900003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Vacuolar Membrane Targeting Protein
creator: Austin Jones
created: 2012-10-01T11:00:00Z
modified: 2015-05-08T01:13:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1165_
m user id: 0
owner id: 13597
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The localizing protein, ZRC1, a vacuolar membrane zinc transporter. C terminal fusion to ZRC1 will localize the complex to the vacuolar membrane.
mutable notes: The gene was codon optimized for yeast and internal restriction sites were removed for to compatibility with our Golden Gate cloning scheme.
mutable provenance: PCR'd from the genome.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K900003_sequence/1

BBa_K392005
type: ComponentDefinition
persistent identity: BBa K392005
display id: BBa_K392005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: yeast SUC2 leader sequence
creator: Tadashi Nakamura, Shuhei Yasumoto, Takahiro Saka, Saya Kakuda
created: 2010-10-09T11:00:00Z
modified: 2015-05-08T01:12:20Z
discontinued: false
dominant: false
experience: experience/None
group u list: _528_
m user id: 0
owner id: 6220
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: LacI promoter with RBS, followed by OmpA outer membrane protein and linker for Escherichia coli cell surface display.
mutable notes: Assembled by 3A method.
mutable provenance: Composite part BBa_K392001 (LacI promoter + RBS) and registry part BBa_K103006 (OmpA w/ linker).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K392005_sequence/1

BBa_K1642007
type: ComponentDefinition
persistent identity: BBa K1642007
display id: BBa_K1642007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ecolivk
creator: Can Yin
created: 2015-08-15T11:00:00Z
modified: 2015-08-16T06:48:24Z
discontinued: false
dominant: false
group u list: _2059_
m user id: 21558
owner id: 24405
owning group id: 9
sample status: Not in stock
status: status/Planning
status: status/Unavailable
bookmark: false
mutable description: a
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence: https://synbiohub.org/public/igem/BBa_K1642007_sequence/1

BBa_K801041
type: ComponentDefinition
persistent identity: BBa K801041
display id: BBa_K801041
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SV40NLS-PhyB-linker-LexA (part for a LexA based light-switchable promoter system)
creator: Dong-Jiunn Jeffery TRUONG
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1057_
m user id: 0
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Composite part of BBa_K801030, BBa_K801030, BBa_K365005, BBa_K801033
mutable notes: -
mutable provenance: Composite part of BBa_K801030, BBa_K801030, BBa_K365005, BBa_K801033
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: Essential Gly for photoconvertible binding of PIF3
sequence annotation: BBa_K801036
sequence annotation: Essential Arg for photoconvertible binding of PIF3
sequence annotation: Essential Cys for chromophore attachement of phycocyanobilin (PCB)
sequence annotation: BBa_K801030
sequence annotation: SV40 nuclear localization sequence (recognized by importin alpha)
sequence annotation: BBa_K801033
sequence annotation: RFC25 fusion scar
sequence annotation: BBa_K365005
sequence annotation: BBa_K801031
sequence: https://synbiohub.org/public/igem/BBa_K801041_sequence/1

Essential Gly for photoconvertible binding of PIF3
type: SequenceAnnotation
persistent identity: annotation2199710
display id: annotation2199710
version: 1
top level: BBa_K801041
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K801041/annotation2199710/range2199710/1

https://synbiohub.org/public/igem/BBa_K801041/annotation2199710/range2199710/1
type: Range
persistent identity: range2199710
display id: range2199710
version: 1
direction: direction/forward
top level: BBa_K801041
start: 391
end: 393
orientation: inline

BBa_K801036
type: SequenceAnnotation
persistent identity: annotation2199718
display id: annotation2199718
version: 1
top level: BBa_K801041
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801041/annotation2199718/range2199718/1

https://synbiohub.org/public/igem/BBa_K801041/annotation2199718/range2199718/1
type: Range
persistent identity: range2199718
display id: range2199718
version: 1
direction: direction/forward
top level: BBa_K801041
start: 2791
end: 3459
orientation: inline

Essential Arg for photoconvertible binding of PIF3
type: SequenceAnnotation
persistent identity: annotation2199712
display id: annotation2199712
version: 1
top level: BBa_K801041
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K801041/annotation2199712/range2199712/1

https://synbiohub.org/public/igem/BBa_K801041/annotation2199712/range2199712/1
type: Range
persistent identity: range2199712
display id: range2199712
version: 1
direction: direction/forward
top level: BBa_K801041
start: 1114
end: 1116
orientation: inline

Essential Cys for chromophore attachement of phycocyanobilin (PCB)
type: SequenceAnnotation
persistent identity: annotation2199713
display id: annotation2199713
version: 1
top level: BBa_K801041
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K801041/annotation2199713/range2199713/1

https://synbiohub.org/public/igem/BBa_K801041/annotation2199713/range2199713/1
type: Range
persistent identity: range2199713
display id: range2199713
version: 1
direction: direction/forward
top level: BBa_K801041
start: 1129
end: 1131
orientation: inline

Essential Arg for photoconvertible binding of PIF3
type: SequenceAnnotation
persistent identity: annotation2199709
display id: annotation2199709
version: 1
top level: BBa_K801041
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K801041/annotation2199709/range2199709/1

https://synbiohub.org/public/igem/BBa_K801041/annotation2199709/range2199709/1
type: Range
persistent identity: range2199709
display id: range2199709
version: 1
direction: direction/forward
top level: BBa_K801041
start: 388
end: 390
orientation: inline

Essential Gly for photoconvertible binding of PIF3
type: SequenceAnnotation
persistent identity: annotation2199711
display id: annotation2199711
version: 1
top level: BBa_K801041
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K801041/annotation2199711/range2199711/1

https://synbiohub.org/public/igem/BBa_K801041/annotation2199711/range2199711/1
type: Range
persistent identity: range2199711
display id: range2199711
version: 1
direction: direction/forward
top level: BBa_K801041
start: 1102
end: 1104
orientation: inline

BBa_K801030
type: SequenceAnnotation
persistent identity: annotation2199708
display id: annotation2199708
version: 1
top level: BBa_K801041
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801041/annotation2199708/range2199708/1

https://synbiohub.org/public/igem/BBa_K801041/annotation2199708/range2199708/1
type: Range
persistent identity: range2199708
display id: range2199708
version: 1
direction: direction/forward
top level: BBa_K801041
start: 1
end: 57
orientation: inline

SV40 nuclear localization sequence (recognized by importin alpha)
type: SequenceAnnotation
persistent identity: annotation2199707
display id: annotation2199707
version: 1
top level: BBa_K801041
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801041/annotation2199707/range2199707/1

https://synbiohub.org/public/igem/BBa_K801041/annotation2199707/range2199707/1
type: Range
persistent identity: range2199707
display id: range2199707
version: 1
direction: direction/forward
top level: BBa_K801041
start: 22
end: 42
orientation: inline

BBa_K801033
type: SequenceAnnotation
persistent identity: annotation2199717
display id: annotation2199717
version: 1
top level: BBa_K801041
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801041/annotation2199717/range2199717/1

https://synbiohub.org/public/igem/BBa_K801041/annotation2199717/range2199717/1
type: Range
persistent identity: range2199717
display id: range2199717
version: 1
direction: direction/forward
top level: BBa_K801041
start: 2857
end: 3459
orientation: inline

RFC25 fusion scar
type: SequenceAnnotation
persistent identity: annotation2199722
display id: annotation2199722
version: 1
top level: BBa_K801041
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801041/annotation2199722/range2199722/1

https://synbiohub.org/public/igem/BBa_K801041/annotation2199722/range2199722/1
type: Range
persistent identity: range2199722
display id: range2199722
version: 1
direction: direction/forward
top level: BBa_K801041
start: 58
end: 63
orientation: inline

BBa_K365005
type: SequenceAnnotation
persistent identity: annotation2199715
display id: annotation2199715
version: 1
top level: BBa_K801041
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801041/annotation2199715/range2199715/1

https://synbiohub.org/public/igem/BBa_K801041/annotation2199715/range2199715/1
type: Range
persistent identity: range2199715
display id: range2199715
version: 1
direction: direction/forward
top level: BBa_K801041
start: 2791
end: 2851
orientation: inline

RFC25 fusion scar
type: SequenceAnnotation
persistent identity: annotation2199724
display id: annotation2199724
version: 1
top level: BBa_K801041
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801041/annotation2199724/range2199724/1

https://synbiohub.org/public/igem/BBa_K801041/annotation2199724/range2199724/1
type: Range
persistent identity: range2199724
display id: range2199724
version: 1
direction: direction/forward
top level: BBa_K801041
start: 2785
end: 2790
orientation: inline

BBa_K801031
type: SequenceAnnotation
persistent identity: annotation2199714
display id: annotation2199714
version: 1
top level: BBa_K801041
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801041/annotation2199714/range2199714/1

https://synbiohub.org/public/igem/BBa_K801041/annotation2199714/range2199714/1
type: Range
persistent identity: range2199714
display id: range2199714
version: 1
direction: direction/forward
top level: BBa_K801041
start: 64
end: 2784
orientation: inline

RFC25 fusion scar
type: SequenceAnnotation
persistent identity: annotation2199716
display id: annotation2199716
version: 1
top level: BBa_K801041
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801041/annotation2199716/range2199716/1

https://synbiohub.org/public/igem/BBa_K801041/annotation2199716/range2199716/1
type: Range
persistent identity: range2199716
display id: range2199716
version: 1
direction: direction/forward
top level: BBa_K801041
start: 2851
end: 2856
orientation: inline

MTS
type: ComponentDefinition
persistent identity: BBa K678025
display id: BBa_K678025
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Mitochondrial targeting sequence for Aspergillus nidulans
creator: DTU-Denmark-2
created: 2011-09-03T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Mitochondrial targeting sequence (MTS) for Aspergillus nidulans. A MTS is usually required for the entry of proteins and intermediary metabolites into mitochondria. The MTS is a DNA fragment encoding the N-terminal 60 amino acids of the AcuJ.
mutable notes: Compatible with the Plug'n'Play assembly standard. This MTS should be placed at the N-terminal of the gene of interest.
mutable provenance: Aspergillus nidulans genomic DNA (AN6279)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence annotation: Linker 2
sequence annotation: MTS
sequence: https://synbiohub.org/public/igem/BBa_K678025_sequence/1

Linker 2
type: SequenceAnnotation
persistent identity: annotation2127379
display id: annotation2127379
version: 1
top level: MTS
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678025/annotation2127379/range2127379/1

https://synbiohub.org/public/igem/BBa_K678025/annotation2127379/range2127379/1
type: Range
persistent identity: range2127379
display id: range2127379
version: 1
direction: direction/forward
top level: MTS
start: 1
end: 8
orientation: inline

MTS
type: SequenceAnnotation
persistent identity: annotation2125850
display id: annotation2125850
version: 1
top level: MTS
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K678025/annotation2125850/range2125850/1

https://synbiohub.org/public/igem/BBa_K678025/annotation2125850/range2125850/1
type: Range
persistent identity: range2125850
display id: range2125850
version: 1
direction: direction/forward
top level: MTS
start: 9
end: 188
orientation: inline

BBa_K801039
type: ComponentDefinition
persistent identity: BBa K801039
display id: BBa_K801039
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SV40NLS-GAL4AD-linker-PIF3 (part for GAL4/LexA based light-switchable promoter system)
creator: Dong-Jiunn Jeffery TRUONG
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1057_
m user id: 0
owner id: 11507
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: CDS for a synthetic hybrid fusion protein. Consists of serveral domains. RFC10 pre and suffix
mutable notes: -
mutable provenance: CDS for a synthetic hybrid fusion protein
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: HA epitope tag
sequence annotation: Linker
sequence annotation: EcoRI/MfeI fusion scar
sequence annotation: SV40 nuclear localization sequence (recognized by importin alpha)
sequence annotation: GAL4 activation domain (GAL4AD)
sequence annotation: T7 promoter (for sequencing)
sequence annotation: PIF3 (First 100 N-terminal amino acids)
sequence: https://synbiohub.org/public/igem/BBa_K801039_sequence/1

HA epitope tag
type: SequenceAnnotation
persistent identity: annotation2200012
display id: annotation2200012
version: 1
top level: BBa_K801039
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801039/annotation2200012/range2200012/1

https://synbiohub.org/public/igem/BBa_K801039/annotation2200012/range2200012/1
type: Range
persistent identity: range2200012
display id: range2200012
version: 1
direction: direction/forward
top level: BBa_K801039
start: 457
end: 477
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2200008
display id: annotation2200008
version: 1
top level: BBa_K801039
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801039/annotation2200008/range2200008/1

https://synbiohub.org/public/igem/BBa_K801039/annotation2200008/range2200008/1
type: Range
persistent identity: range2200008
display id: range2200008
version: 1
direction: direction/forward
top level: BBa_K801039
start: 409
end: 498
orientation: inline

EcoRI/MfeI fusion scar
type: SequenceAnnotation
persistent identity: annotation2200000
display id: annotation2200000
version: 1
top level: BBa_K801039
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801039/annotation2200000/range2200000/1

https://synbiohub.org/public/igem/BBa_K801039/annotation2200000/range2200000/1
type: Range
persistent identity: range2200000
display id: range2200000
version: 1
direction: direction/forward
top level: BBa_K801039
start: 499
end: 504
orientation: inline

SV40 nuclear localization sequence (recognized by importin alpha)
type: SequenceAnnotation
persistent identity: annotation2199929
display id: annotation2199929
version: 1
top level: BBa_K801039
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801039/annotation2199929/range2199929/1

https://synbiohub.org/public/igem/BBa_K801039/annotation2199929/range2199929/1
type: Range
persistent identity: range2199929
display id: range2199929
version: 1
direction: direction/forward
top level: BBa_K801039
start: 31
end: 51
orientation: inline

GAL4 activation domain (GAL4AD)
type: SequenceAnnotation
persistent identity: annotation2199997
display id: annotation2199997
version: 1
top level: BBa_K801039
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K801039/annotation2199997/range2199997/1

https://synbiohub.org/public/igem/BBa_K801039/annotation2199997/range2199997/1
type: Range
persistent identity: range2199997
display id: range2199997
version: 1
direction: direction/forward
top level: BBa_K801039
start: 70
end: 408
orientation: inline

T7 promoter (for sequencing)
type: SequenceAnnotation
persistent identity: annotation2200010
display id: annotation2200010
version: 1
top level: BBa_K801039
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K801039/annotation2200010/range2200010/1

https://synbiohub.org/public/igem/BBa_K801039/annotation2200010/range2200010/1
type: Range
persistent identity: range2200010
display id: range2200010
version: 1
direction: direction/forward
top level: BBa_K801039
start: 414
end: 436
orientation: inline

PIF3 (First 100 N-terminal amino acids)
type: SequenceAnnotation
persistent identity: annotation2199998
display id: annotation2199998
version: 1
top level: BBa_K801039
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K801039/annotation2199998/range2199998/1

https://synbiohub.org/public/igem/BBa_K801039/annotation2199998/range2199998/1
type: Range
persistent identity: range2199998
display id: range2199998
version: 1
direction: direction/forward
top level: BBa_K801039
start: 503
end: 801
orientation: inline

BBa_K1478000
type: ComponentDefinition
persistent identity: BBa K1478000
display id: BBa_K1478000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Expa4 plant secretion signal, localizes to extracellular space
creator: Fabian Fr??mling
created: 2014-09-12T11:00:00Z
modified: 2015-05-27T02:12:26Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1858_
m user id: 4206
owner id: 20973
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Coding region for first 20 aminoacids of plant protein Exansin4. Secretion signal for plants (derived from A. thaliana). When fused to a protein, directs the protein to extracellular space.
mutable notes: Sequence was optimized for Escherichia coli and Arabidopsis thaliana codon usage.
mutable provenance: Derived from Expansin4 from Arabidopsis thaliana(NP_181500).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K1478000_sequence/1

BBa_K1909003
type: ComponentDefinition
persistent identity: BBa K1909003
display id: BBa_K1909003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: EnvZ Signal Peptide and first Transmembrane Domain
creator: Daniel Wedemeyer
created: 2016-10-13T11:00:00Z
modified: 2016-10-14T11:06:33Z
discontinued: false
dominant: false
group u list: _2375_
m user id: 29525
owner id: 29525
owning group id: 9
status: status/Planning
bookmark: false
mutable description: For EnvZ fusion protein design. Long description will be added soon.
mutable notes: coming soon
mutable provenance: Source: E. coli EnvZ protein (ncbi: https://www.ncbi.nlm.nih.gov/gene/947272)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: EnvZ
sequence annotation: FLAG
sequence: https://synbiohub.org/public/igem/BBa_K1909003_sequence/1

EnvZ
type: SequenceAnnotation
persistent identity: annotation2507125
display id: annotation2507125
version: 1
top level: BBa_K1909003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1909003/annotation2507125/range2507125/1

https://synbiohub.org/public/igem/BBa_K1909003/annotation2507125/range2507125/1
type: Range
persistent identity: range2507125
display id: range2507125
version: 1
direction: direction/forward
top level: BBa_K1909003
start: 1
end: 161
orientation: inline

FLAG
type: SequenceAnnotation
persistent identity: annotation2507149
display id: annotation2507149
version: 1
top level: BBa_K1909003
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1909003/annotation2507149/range2507149/1

https://synbiohub.org/public/igem/BBa_K1909003/annotation2507149/range2507149/1
type: Range
persistent identity: range2507149
display id: range2507149
version: 1
direction: direction/forward
top level: BBa_K1909003
start: 46
end: 69
orientation: inline

BBa_K801040
type: ComponentDefinition
persistent identity: BBa K801040
display id: BBa_K801040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SV40NLS-PhyB-linker-Gal4DBD (part for GAL4 based light switchable promoter system)
creator: Dong-Jiunn Jeffery TRUONG
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1057_
m user id: 0
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Composite part of BBa_K801030, BBa_K801030, BBa_K365005, BBa_K801035
mutable notes: -
mutable provenance: Composite part of BBa_K801030, BBa_K801030, BBa_K365005, BBa_K801035
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Essential Gly for photoconvertible binding of PIF3
sequence annotation: RFC25 fusion scar
sequence annotation: Essential Arg for photoconvertible binding of PIF3
sequence annotation: BBa_K801032
sequence annotation: Essential Cys for chromophore attachement of phycocyanobilin (PCB)
sequence annotation: BBa_K801037
sequence annotation: BBa_K801038
sequence annotation: BBa_K801030
sequence annotation: BBa_K365005
sequence annotation: BBa_K801031
sequence annotation: SV40 nuclear localization sequence (recognized by importin alpha)
sequence: https://synbiohub.org/public/igem/BBa_K801040_sequence/1

Essential Gly for photoconvertible binding of PIF3
type: SequenceAnnotation
persistent identity: annotation2199582
display id: annotation2199582
version: 1
top level: BBa_K801040
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K801040/annotation2199582/range2199582/1

https://synbiohub.org/public/igem/BBa_K801040/annotation2199582/range2199582/1
type: Range
persistent identity: range2199582
display id: range2199582
version: 1
direction: direction/forward
top level: BBa_K801040
start: 391
end: 393
orientation: inline

Essential Gly for photoconvertible binding of PIF3
type: SequenceAnnotation
persistent identity: annotation2199583
display id: annotation2199583
version: 1
top level: BBa_K801040
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K801040/annotation2199583/range2199583/1

https://synbiohub.org/public/igem/BBa_K801040/annotation2199583/range2199583/1
type: Range
persistent identity: range2199583
display id: range2199583
version: 1
direction: direction/forward
top level: BBa_K801040
start: 1102
end: 1104
orientation: inline

RFC25 fusion scar
type: SequenceAnnotation
persistent identity: annotation2199586
display id: annotation2199586
version: 1
top level: BBa_K801040
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801040/annotation2199586/range2199586/1

https://synbiohub.org/public/igem/BBa_K801040/annotation2199586/range2199586/1
type: Range
persistent identity: range2199586
display id: range2199586
version: 1
direction: direction/forward
top level: BBa_K801040
start: 2785
end: 2790
orientation: inline

Essential Arg for photoconvertible binding of PIF3
type: SequenceAnnotation
persistent identity: annotation2199584
display id: annotation2199584
version: 1
top level: BBa_K801040
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K801040/annotation2199584/range2199584/1

https://synbiohub.org/public/igem/BBa_K801040/annotation2199584/range2199584/1
type: Range
persistent identity: range2199584
display id: range2199584
version: 1
direction: direction/forward
top level: BBa_K801040
start: 1114
end: 1116
orientation: inline

BBa_K801032
type: SequenceAnnotation
persistent identity: annotation2199590
display id: annotation2199590
version: 1
top level: BBa_K801040
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801040/annotation2199590/range2199590/1

https://synbiohub.org/public/igem/BBa_K801040/annotation2199590/range2199590/1
type: Range
persistent identity: range2199590
display id: range2199590
version: 1
direction: direction/forward
top level: BBa_K801040
start: 2857
end: 3294
orientation: inline

Essential Cys for chromophore attachement of phycocyanobilin (PCB)
type: SequenceAnnotation
persistent identity: annotation2199585
display id: annotation2199585
version: 1
top level: BBa_K801040
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K801040/annotation2199585/range2199585/1

https://synbiohub.org/public/igem/BBa_K801040/annotation2199585/range2199585/1
type: Range
persistent identity: range2199585
display id: range2199585
version: 1
direction: direction/forward
top level: BBa_K801040
start: 1129
end: 1131
orientation: inline

BBa_K801037
type: SequenceAnnotation
persistent identity: annotation2199588
display id: annotation2199588
version: 1
top level: BBa_K801040
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801040/annotation2199588/range2199588/1

https://synbiohub.org/public/igem/BBa_K801040/annotation2199588/range2199588/1
type: Range
persistent identity: range2199588
display id: range2199588
version: 1
direction: direction/forward
top level: BBa_K801040
start: 2791
end: 3294
orientation: inline

BBa_K801038
type: SequenceAnnotation
persistent identity: annotation2199591
display id: annotation2199591
version: 1
top level: BBa_K801040
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801040/annotation2199591/range2199591/1

https://synbiohub.org/public/igem/BBa_K801040/annotation2199591/range2199591/1
type: Range
persistent identity: range2199591
display id: range2199591
version: 1
direction: direction/forward
top level: BBa_K801040
start: 64
end: 3294
orientation: inline

BBa_K801030
type: SequenceAnnotation
persistent identity: annotation2199579
display id: annotation2199579
version: 1
top level: BBa_K801040
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801040/annotation2199579/range2199579/1

https://synbiohub.org/public/igem/BBa_K801040/annotation2199579/range2199579/1
type: Range
persistent identity: range2199579
display id: range2199579
version: 1
direction: direction/reverse
top level: BBa_K801040
start: 1
end: 57
orientation: inline

Essential Arg for photoconvertible binding of PIF3
type: SequenceAnnotation
persistent identity: annotation2199581
display id: annotation2199581
version: 1
top level: BBa_K801040
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K801040/annotation2199581/range2199581/1

https://synbiohub.org/public/igem/BBa_K801040/annotation2199581/range2199581/1
type: Range
persistent identity: range2199581
display id: range2199581
version: 1
direction: direction/forward
top level: BBa_K801040
start: 388
end: 390
orientation: inline

BBa_K365005
type: SequenceAnnotation
persistent identity: annotation2199587
display id: annotation2199587
version: 1
top level: BBa_K801040
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801040/annotation2199587/range2199587/1

https://synbiohub.org/public/igem/BBa_K801040/annotation2199587/range2199587/1
type: Range
persistent identity: range2199587
display id: range2199587
version: 1
direction: direction/forward
top level: BBa_K801040
start: 2791
end: 2850
orientation: inline

BBa_K801031
type: SequenceAnnotation
persistent identity: annotation2199580
display id: annotation2199580
version: 1
top level: BBa_K801040
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801040/annotation2199580/range2199580/1

https://synbiohub.org/public/igem/BBa_K801040/annotation2199580/range2199580/1
type: Range
persistent identity: range2199580
display id: range2199580
version: 1
direction: direction/forward
top level: BBa_K801040
start: 64
end: 2784
orientation: inline

RFC25 fusion scar
type: SequenceAnnotation
persistent identity: annotation2199589
display id: annotation2199589
version: 1
top level: BBa_K801040
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801040/annotation2199589/range2199589/1

https://synbiohub.org/public/igem/BBa_K801040/annotation2199589/range2199589/1
type: Range
persistent identity: range2199589
display id: range2199589
version: 1
direction: direction/forward
top level: BBa_K801040
start: 2851
end: 2856
orientation: inline

SV40 nuclear localization sequence (recognized by importin alpha)
type: SequenceAnnotation
persistent identity: annotation2199578
display id: annotation2199578
version: 1
top level: BBa_K801040
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801040/annotation2199578/range2199578/1

https://synbiohub.org/public/igem/BBa_K801040/annotation2199578/range2199578/1
type: Range
persistent identity: range2199578
display id: range2199578
version: 1
direction: direction/forward
top level: BBa_K801040
start: 22
end: 42
orientation: inline

eGFP-PTS1
type: ComponentDefinition
persistent identity: BBa K678002
display id: BBa_K678002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: eGFP-PTS1 for mammalian cells
creator: DTU-Denmark-2
created: 2011-09-01T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Vector expressing eGFP from the CMV promoter with the BGH polyA tail and with a hygromycin resistance cassette
mutable notes: Compatible with the Plug'n'Play with DNA assembly system.
mutable provenance: Source to be announced
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: Kozak
sequence annotation: Linker 2
sequence annotation: Linker 1
sequence annotation: Linker 5
sequence annotation: PTS1
sequence annotation: CMV
sequence annotation: Linker 6
sequence annotation: eGFP
sequence annotation: Linker 4
sequence annotation: Hygromycin cassette
sequence annotation: BGH pA
sequence: https://synbiohub.org/public/igem/BBa_K678002_sequence/1

Kozak
type: SequenceAnnotation
persistent identity: annotation2126935
display id: annotation2126935
version: 1
top level: eGFP-PTS1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678002/annotation2126935/range2126935/1

https://synbiohub.org/public/igem/BBa_K678002/annotation2126935/range2126935/1
type: Range
persistent identity: range2126935
display id: range2126935
version: 1
direction: direction/forward
top level: eGFP-PTS1
start: 672
end: 682
orientation: inline

Linker 2
type: SequenceAnnotation
persistent identity: annotation2126914
display id: annotation2126914
version: 1
top level: eGFP-PTS1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678002/annotation2126914/range2126914/1

https://synbiohub.org/public/igem/BBa_K678002/annotation2126914/range2126914/1
type: Range
persistent identity: range2126914
display id: range2126914
version: 1
direction: direction/forward
top level: eGFP-PTS1
start: 663
end: 671
orientation: inline

Linker 1
type: SequenceAnnotation
persistent identity: annotation2126912
display id: annotation2126912
version: 1
top level: eGFP-PTS1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678002/annotation2126912/range2126912/1

https://synbiohub.org/public/igem/BBa_K678002/annotation2126912/range2126912/1
type: Range
persistent identity: range2126912
display id: range2126912
version: 1
direction: direction/forward
top level: eGFP-PTS1
start: 1
end: 8
orientation: inline

Linker 5
type: SequenceAnnotation
persistent identity: annotation2126939
display id: annotation2126939
version: 1
top level: eGFP-PTS1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678002/annotation2126939/range2126939/1

https://synbiohub.org/public/igem/BBa_K678002/annotation2126939/range2126939/1
type: Range
persistent identity: range2126939
display id: range2126939
version: 1
direction: direction/forward
top level: eGFP-PTS1
start: 1640
end: 1647
orientation: inline

PTS1
type: SequenceAnnotation
persistent identity: annotation2126936
display id: annotation2126936
version: 1
top level: eGFP-PTS1
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K678002/annotation2126936/range2126936/1

https://synbiohub.org/public/igem/BBa_K678002/annotation2126936/range2126936/1
type: Range
persistent identity: range2126936
display id: range2126936
version: 1
direction: direction/forward
top level: eGFP-PTS1
start: 1395
end: 1406
orientation: inline

CMV
type: SequenceAnnotation
persistent identity: annotation2126913
display id: annotation2126913
version: 1
top level: eGFP-PTS1
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K678002/annotation2126913/range2126913/1

https://synbiohub.org/public/igem/BBa_K678002/annotation2126913/range2126913/1
type: Range
persistent identity: range2126913
display id: range2126913
version: 1
direction: direction/forward
top level: eGFP-PTS1
start: 9
end: 662
orientation: inline

Linker 6
type: SequenceAnnotation
persistent identity: annotation2126941
display id: annotation2126941
version: 1
top level: eGFP-PTS1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678002/annotation2126941/range2126941/1

https://synbiohub.org/public/igem/BBa_K678002/annotation2126941/range2126941/1
type: Range
persistent identity: range2126941
display id: range2126941
version: 1
direction: direction/forward
top level: eGFP-PTS1
start: 3399
end: 3406
orientation: inline

eGFP
type: SequenceAnnotation
persistent identity: annotation2126934
display id: annotation2126934
version: 1
top level: eGFP-PTS1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K678002/annotation2126934/range2126934/1

https://synbiohub.org/public/igem/BBa_K678002/annotation2126934/range2126934/1
type: Range
persistent identity: range2126934
display id: range2126934
version: 1
direction: direction/forward
top level: eGFP-PTS1
start: 679
end: 1394
orientation: inline

Linker 4
type: SequenceAnnotation
persistent identity: annotation2126937
display id: annotation2126937
version: 1
top level: eGFP-PTS1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678002/annotation2126937/range2126937/1

https://synbiohub.org/public/igem/BBa_K678002/annotation2126937/range2126937/1
type: Range
persistent identity: range2126937
display id: range2126937
version: 1
direction: direction/forward
top level: eGFP-PTS1
start: 1407
end: 1414
orientation: inline

Hygromycin cassette
type: SequenceAnnotation
persistent identity: annotation2126940
display id: annotation2126940
version: 1
top level: eGFP-PTS1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678002/annotation2126940/range2126940/1

https://synbiohub.org/public/igem/BBa_K678002/annotation2126940/range2126940/1
type: Range
persistent identity: range2126940
display id: range2126940
version: 1
direction: direction/forward
top level: eGFP-PTS1
start: 1648
end: 3398
orientation: inline

BGH pA
type: SequenceAnnotation
persistent identity: annotation2126938
display id: annotation2126938
version: 1
top level: eGFP-PTS1
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K678002/annotation2126938/range2126938/1

https://synbiohub.org/public/igem/BBa_K678002/annotation2126938/range2126938/1
type: Range
persistent identity: range2126938
display id: range2126938
version: 1
direction: direction/forward
top level: eGFP-PTS1
start: 1415
end: 1639
orientation: inline

slr2016 SS
type: ComponentDefinition
persistent identity: BBa K125310
display id: BBa_K125310
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: slr2016 signal sequence from cyanobacterium Synechocystis; secretes protein
creator: Krystle Salazar and Grace Kwan
created: 2008-07-24T11:00:00Z
modified: 2015-05-08T01:09:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _179_
m user id: 0
owner id: 3229
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable provenance: The ''slr2016'' signal sequence is located at the amino terminus of slr2016 polypeptides synthesized by ''Synechocystis'' sp. PCC 6803.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence annotation: slr2016 signal sequence
sequence: https://synbiohub.org/public/igem/BBa_K125310_sequence/1

slr2016 signal sequence
type: SequenceAnnotation
persistent identity: annotation1968259
display id: annotation1968259
version: 1
top level: slr2016 SS
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K125310/annotation1968259/range1968259/1

https://synbiohub.org/public/igem/BBa_K125310/annotation1968259/range1968259/1
type: Range
persistent identity: range1968259
display id: range1968259
version: 1
direction: direction/forward
top level: slr2016 SS
start: 1
end: 53
orientation: inline

mRFP1-NLS
type: ComponentDefinition
persistent identity: BBa K678034
display id: BBa_K678034
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mRFP1-NLS, targeted to the nucleus
creator: DTU-Denmark-2
created: 2011-09-09T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: mRFP
mutable notes: This part was designed for the Plug'n'Play with DNA assembly standard (RFC 80), therefore this part contains tails at the 5' and 3' ends as shown on the figure below. Please notice that mRFP1-NLS is not the same as BBa_K678030, BBa_K678031, or BBa_K678032.
mutable provenance: From p71-mRFP1 of the Center for Microbial Biotechnology plasmid collection at the Technical University of Denmark. The NLS sequence was synthesized.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: stop
sequence annotation: RFP
sequence annotation: NLS
sequence annotation: start
sequence annotation: Linker 2
sequence: https://synbiohub.org/public/igem/BBa_K678034_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2126905
display id: annotation2126905
version: 1
top level: mRFP1-NLS
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K678034/annotation2126905/range2126905/1

https://synbiohub.org/public/igem/BBa_K678034/annotation2126905/range2126905/1
type: Range
persistent identity: range2126905
display id: range2126905
version: 1
direction: direction/forward
top level: mRFP1-NLS
start: 705
end: 707
orientation: inline

RFP
type: SequenceAnnotation
persistent identity: annotation2127400
display id: annotation2127400
version: 1
top level: mRFP1-NLS
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K678034/annotation2127400/range2127400/1

https://synbiohub.org/public/igem/BBa_K678034/annotation2127400/range2127400/1
type: Range
persistent identity: range2127400
display id: range2127400
version: 1
direction: direction/forward
top level: mRFP1-NLS
start: 9
end: 683
orientation: inline

NLS
type: SequenceAnnotation
persistent identity: annotation2126903
display id: annotation2126903
version: 1
top level: mRFP1-NLS
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678034/annotation2126903/range2126903/1

https://synbiohub.org/public/igem/BBa_K678034/annotation2126903/range2126903/1
type: Range
persistent identity: range2126903
display id: range2126903
version: 1
direction: direction/forward
top level: mRFP1-NLS
start: 684
end: 704
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2126906
display id: annotation2126906
version: 1
top level: mRFP1-NLS
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K678034/annotation2126906/range2126906/1

https://synbiohub.org/public/igem/BBa_K678034/annotation2126906/range2126906/1
type: Range
persistent identity: range2126906
display id: range2126906
version: 1
direction: direction/forward
top level: mRFP1-NLS
start: 9
end: 11
orientation: inline

Linker 2
type: SequenceAnnotation
persistent identity: annotation2126904
display id: annotation2126904
version: 1
top level: mRFP1-NLS
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678034/annotation2126904/range2126904/1

https://synbiohub.org/public/igem/BBa_K678034/annotation2126904/range2126904/1
type: Range
persistent identity: range2126904
display id: range2126904
version: 1
direction: direction/forward
top level: mRFP1-NLS
start: 1
end: 8
orientation: inline

BBa_K1642008
type: ComponentDefinition
persistent identity: BBa K1642008
display id: BBa_K1642008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Sll1338
creator: Can Yin
created: 2015-08-15T11:00:00Z
modified: 2015-08-16T06:16:24Z
discontinued: false
dominant: false
group u list: _2059_
m user id: 21558
owner id: 24405
owning group id: 9
sample status: Not in stock
status: status/Planning
status: status/Unavailable
bookmark: false
mutable description: a
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence: https://synbiohub.org/public/igem/BBa_K1642008_sequence/1

BBa_K416008
type: ComponentDefinition
persistent identity: BBa K416008
display id: BBa_K416008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Aga2 fused to eCFP
creator: Russell Durrett
created: 2010-10-26T11:00:00Z
modified: 2015-05-08T01:12:27Z
discontinued: false
dominant: false
experience: experience/None
group u list: _527_
m user id: 0
owner id: 6443
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: When working correctly this reporter construct transcribes Aga2-eCFP from the Gal promoter. If the yeast strain is also expressing Aga1, then this fusion should be anchored onto the plasma membrane, allowing you to visualize eCFP as a halo around the yeast cell.
mutable notes: We considered fusing this reporter to a variety of promoters, but in the end chose pGAL because it gives a very high level of transcription and we could turn the reporter on and off
mutable provenance: This part comes from a mixture of biobricks that were received in the 2010 initial distribution and from parts that NYU iGEM 2010 made ourselves.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K416005
component: BBa_K106018
component: BBa_K105009
component: BBa_K416000
component: BBa_K416001
sequence annotation: BBa_K416005
sequence annotation: BBa_K106018
sequence annotation: BBa_K416000
sequence annotation: BBa_K105009
sequence annotation: BBa_K416001
sequence: https://synbiohub.org/public/igem/BBa_K416008_sequence/1

BBa_K416005
type: Component
persistent identity: component2110283
display id: component2110283
version: 1
top level: BBa_K416008
access: public
definition: https://synbiohub.org/public/igem/BBa_K416005/1

BBa_K106018
type: Component
persistent identity: component2110288
display id: component2110288
version: 1
top level: BBa_K416008
access: public
definition: https://synbiohub.org/public/igem/BBa_K106018/1

BBa_K105009
type: Component
persistent identity: component2110287
display id: component2110287
version: 1
top level: BBa_K416008
access: public
definition: https://synbiohub.org/public/igem/BBa_K105009/1

BBa_K416000
type: Component
persistent identity: component2110285
display id: component2110285
version: 1
top level: BBa_K416008
access: public
definition: BBa_K416000

BBa_K416001
type: Component
persistent identity: component2110286
display id: component2110286
version: 1
top level: BBa_K416008
access: public
definition: https://synbiohub.org/public/igem/BBa_K416001/1

BBa_K416005
type: SequenceAnnotation
persistent identity: annotation2110283
display id: annotation2110283
version: 1
top level: BBa_K416008
location: https://synbiohub.org/public/igem/BBa_K416008/annotation2110283/range2110283/1
component: BBa_K416005

https://synbiohub.org/public/igem/BBa_K416008/annotation2110283/range2110283/1
type: Range
persistent identity: range2110283
display id: range2110283
version: 1
direction: direction/reverse
top level: BBa_K416008
start: 1
end: 575
orientation: inline

BBa_K106018
type: SequenceAnnotation
persistent identity: annotation2110288
display id: annotation2110288
version: 1
top level: BBa_K416008
location: https://synbiohub.org/public/igem/BBa_K416008/annotation2110288/range2110288/1
component: BBa_K106018

https://synbiohub.org/public/igem/BBa_K416008/annotation2110288/range2110288/1
type: Range
persistent identity: range2110288
display id: range2110288
version: 1
direction: direction/reverse
top level: BBa_K416008
start: 1656
end: 1835
orientation: inline

BBa_K416000
type: SequenceAnnotation
persistent identity: annotation2110285
display id: annotation2110285
version: 1
top level: BBa_K416008
location: https://synbiohub.org/public/igem/BBa_K416008/annotation2110285/range2110285/1
component: BBa_K416000

https://synbiohub.org/public/igem/BBa_K416008/annotation2110285/range2110285/1
type: Range
persistent identity: range2110285
display id: range2110285
version: 1
direction: direction/reverse
top level: BBa_K416008
start: 582
end: 842
orientation: inline

BBa_K105009
type: SequenceAnnotation
persistent identity: annotation2110287
display id: annotation2110287
version: 1
top level: BBa_K416008
location: https://synbiohub.org/public/igem/BBa_K416008/annotation2110287/range2110287/1
component: BBa_K105009

https://synbiohub.org/public/igem/BBa_K416008/annotation2110287/range2110287/1
type: Range
persistent identity: range2110287
display id: range2110287
version: 1
direction: direction/reverse
top level: BBa_K416008
start: 904
end: 1647
orientation: inline

BBa_K416001
type: SequenceAnnotation
persistent identity: annotation2110286
display id: annotation2110286
version: 1
top level: BBa_K416008
location: https://synbiohub.org/public/igem/BBa_K416008/annotation2110286/range2110286/1
component: BBa_K416001

https://synbiohub.org/public/igem/BBa_K416008/annotation2110286/range2110286/1
type: Range
persistent identity: range2110286
display id: range2110286
version: 1
direction: direction/reverse
top level: BBa_K416008
start: 851
end: 895
orientation: inline

myristoila
type: ComponentDefinition
persistent identity: BBa I712026
display id: BBa_I712026
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: myristoilation signal sequence; anchors protein to membrane
creator: Katja Kolar
created: 2007-10-22T11:00:00Z
modified: 2015-08-31T04:07:46Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _130_
m user id: 0
owner id: 1883
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Signal sequence for myristoilation.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence: https://synbiohub.org/public/igem/BBa_I712026_sequence/1

pilA1 SS
type: ComponentDefinition
persistent identity: BBa K125300
display id: BBa_K125300
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pilA1 signal sequence from cyanobacterium Synechocystis; secretes protein
creator: Krystle Salazar and Grace Kwan
created: 2008-07-24T11:00:00Z
modified: 2015-05-08T01:09:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _179_
m user id: 0
owner id: 3229
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: ''pilA'' is a pilin protein known to be secreted by the cyanobacteria ''Synechocystis'' sp. PCC6803.
mutable provenance: The ''pilA'' signal sequence is found at the amino terminal of ''pilA'' polypeptides synthesized by ''Synechocystis'' sp. PCC6803.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence annotation: pilA signal sequence
sequence: https://synbiohub.org/public/igem/BBa_K125300_sequence/1

pilA signal sequence
type: SequenceAnnotation
persistent identity: annotation1968258
display id: annotation1968258
version: 1
top level: pilA1 SS
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K125300/annotation1968258/range1968258/1

https://synbiohub.org/public/igem/BBa_K125300/annotation1968258/range1968258/1
type: Range
persistent identity: range1968258
display id: range1968258
version: 1
direction: direction/forward
top level: pilA1 SS
start: 1
end: 62
orientation: inline

BBa_K1732000
type: ComponentDefinition
persistent identity: BBa K1732000
display id: BBa_K1732000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23100-PelB-GlucHCO-B0015
creator: Donna Lee
created: 2015-09-02T11:00:00Z
modified: 2015-09-18T01:01:25Z
discontinued: false
dominant: false
group u list: _2154_
m user id: 27195
owner id: 27195
owning group id: 9
status: status/Planning
bookmark: false
mutable description: J23100 Promoter with a RBS (BBa_S04423), PelB Leader sequence, Gaussia princeps luciferase, and a B0015 terminator. Allows Gaussia princeps luciferase to be transcribed and translated using a strong promoter. Luminescence can be expressed by reacting the luciferase product with coelenterazine.
mutable notes: This sequence is codon optimized for E.coli. Sequence contains a PelB leader sequence that allows the Gaussia princeps luciferase proteins to secreted into the periplasmic space of the cell.
mutable provenance: Engineered from XXXXXXXX. Provided by the Bruchez lab at Carnegie Mellon.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
component: BBa_J23100
component: BBa_K208004
component: BBa_B0034
component: BBa_K1732027
component: BBa_B0015
sequence annotation: BBa_K208004
sequence annotation: BBa_B0034
sequence annotation: BBa_B0015
sequence annotation: BBa_J23100
sequence annotation: BBa_K1732027
sequence: https://synbiohub.org/public/igem/BBa_K1732000_sequence/1

BBa_J23100
type: Component
persistent identity: component2469775
display id: component2469775
version: 1
top level: BBa_K1732000
access: public
definition: https://synbiohub.org/public/igem/BBa_J23100/1

BBa_K208004
type: Component
persistent identity: component2469780
display id: component2469780
version: 1
top level: BBa_K1732000
access: public
definition: https://synbiohub.org/public/igem/BBa_K208004/1

BBa_B0034
type: Component
persistent identity: component2469777
display id: component2469777
version: 1
top level: BBa_K1732000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1732027
type: Component
persistent identity: component2469783
display id: component2469783
version: 1
top level: BBa_K1732000
access: public
definition: https://synbiohub.org/public/igem/BBa_K1732027/1

BBa_B0015
type: Component
persistent identity: component2469790
display id: component2469790
version: 1
top level: BBa_K1732000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K208004
type: SequenceAnnotation
persistent identity: annotation2469780
display id: annotation2469780
version: 1
top level: BBa_K1732000
location: https://synbiohub.org/public/igem/BBa_K1732000/annotation2469780/range2469780/1
component: BBa_K208004

https://synbiohub.org/public/igem/BBa_K1732000/annotation2469780/range2469780/1
type: Range
persistent identity: range2469780
display id: range2469780
version: 1
direction: direction/reverse
top level: BBa_K1732000
start: 62
end: 127
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2469777
display id: annotation2469777
version: 1
top level: BBa_K1732000
location: https://synbiohub.org/public/igem/BBa_K1732000/annotation2469777/range2469777/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1732000/annotation2469777/range2469777/1
type: Range
persistent identity: range2469777
display id: range2469777
version: 1
direction: direction/reverse
top level: BBa_K1732000
start: 44
end: 55
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2469790
display id: annotation2469790
version: 1
top level: BBa_K1732000
location: https://synbiohub.org/public/igem/BBa_K1732000/annotation2469790/range2469790/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1732000/annotation2469790/range2469790/1
type: Range
persistent identity: range2469790
display id: range2469790
version: 1
direction: direction/reverse
top level: BBa_K1732000
start: 666
end: 794
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2469775
display id: annotation2469775
version: 1
top level: BBa_K1732000
location: https://synbiohub.org/public/igem/BBa_K1732000/annotation2469775/range2469775/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K1732000/annotation2469775/range2469775/1
type: Range
persistent identity: range2469775
display id: range2469775
version: 1
direction: direction/reverse
top level: BBa_K1732000
start: 1
end: 35
orientation: inline

BBa_K1732027
type: SequenceAnnotation
persistent identity: annotation2469783
display id: annotation2469783
version: 1
top level: BBa_K1732000
location: https://synbiohub.org/public/igem/BBa_K1732000/annotation2469783/range2469783/1
component: BBa_K1732027

https://synbiohub.org/public/igem/BBa_K1732000/annotation2469783/range2469783/1
type: Range
persistent identity: range2469783
display id: range2469783
version: 1
direction: direction/reverse
top level: BBa_K1732000
start: 136
end: 657
orientation: inline

MLS
type: ComponentDefinition
persistent identity: BBa K1119000
display id: BBa_K1119000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Mitochondrial Leader Sequence in RFC10 standard
creator: Wai In Hui
created: 2013-09-10T11:00:00Z
modified: 2015-05-08T01:09:13Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1430_
m user id: 0
owner id: 16863
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Mitochondrial leader sequence (MLS) helps direct protein to the mitochondria. When this sequence is cloned in frame at the N-terminus of the protein, it can target the recombinant protein to the mitochondria in mammalian cells. MLS will be removed upon translocation into the mitochondrial matrix. However, at least four additional amino acids (Ile-His-Ser-Leu) on this sequence are known to be retained on the N-terminus of the protein.
mutable notes: No
mutable provenance: Plasmid from Invitrogen: pCMV/myc/mito (http://tools.lifetechnologies.com/content/sfs/manuals/pshooter_pcmv_man.pdf)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: Mitochondrial Leader Sequence
sequence: https://synbiohub.org/public/igem/BBa_K1119000_sequence/1

Mitochondrial Leader Sequence
type: SequenceAnnotation
persistent identity: annotation2363256
display id: annotation2363256
version: 1
top level: MLS
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1119000/annotation2363256/range2363256/1

https://synbiohub.org/public/igem/BBa_K1119000/annotation2363256/range2363256/1
type: Range
persistent identity: range2363256
display id: range2363256
version: 1
direction: direction/forward
top level: MLS
start: 1
end: 87
orientation: inline

MLS
type: ComponentDefinition
persistent identity: BBa K1119001
display id: BBa_K1119001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Mitochondrial Leader Sequence with RFC25 standard
creator: Wai In Hui
created: 2013-09-10T11:00:00Z
modified: 2015-05-08T01:09:13Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1430_
m user id: 0
owner id: 16863
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Mitochondrial leader sequence (MLS) helps direct protein to the mitochondria. When this sequence is cloned in frame at the N-terminus of the protein, it can target the recombinant protein to the mitochondria in mammalian cells. MLS will be removed upon translocation into the mitochondrial matrix. However, at least four additional amino acids (Ile-His-Ser-Leu) on this sequence are known to be retained on the N-terminus of the protein. Since MLS always fuses with protein for function, this BioBrick is in RFC 25 standard, which facilitate protein fusion in order to introduce other protein into mitochondria.
mutable notes: No
mutable provenance: Invitrogen: pCMV/myc/mito (http://tools.lifetechnologies.com/content/sfs/manuals/pshooter_pcmv_man.pdf)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: Mitochondrial Leader Sequence
sequence: https://synbiohub.org/public/igem/BBa_K1119001_sequence/1

Mitochondrial Leader Sequence
type: SequenceAnnotation
persistent identity: annotation2363257
display id: annotation2363257
version: 1
top level: MLS
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1119001/annotation2363257/range2363257/1

https://synbiohub.org/public/igem/BBa_K1119001/annotation2363257/range2363257/1
type: Range
persistent identity: range2363257
display id: range2363257
version: 1
direction: direction/forward
top level: MLS
start: 1
end: 87
orientation: inline

GFP-PTS1
type: ComponentDefinition
persistent identity: BBa K678033
display id: BBa_K678033
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP-PTS1
creator: DTU-Denmark-2
created: 2011-09-10T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: GFP targeted to the peroxisomes of Aspergillus nidulans
mutable notes: Design considerations This part was designed for the Plug'n'Play with DNA assembly standard (RFC 80), therefore this part contains linkers at the 5' and 3' ends as shown on the figure below.
mutable provenance: to be announced
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: PTS1
sequence annotation: Linker 2
sequence annotation: stop
sequence annotation: GFP
sequence: https://synbiohub.org/public/igem/BBa_K678033_sequence/1

PTS1
type: SequenceAnnotation
persistent identity: annotation2127397
display id: annotation2127397
version: 1
top level: GFP-PTS1
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K678033/annotation2127397/range2127397/1

https://synbiohub.org/public/igem/BBa_K678033/annotation2127397/range2127397/1
type: Range
persistent identity: range2127397
display id: range2127397
version: 1
direction: direction/forward
top level: GFP-PTS1
start: 726
end: 734
orientation: inline

Linker 2
type: SequenceAnnotation
persistent identity: annotation2127395
display id: annotation2127395
version: 1
top level: GFP-PTS1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678033/annotation2127395/range2127395/1

https://synbiohub.org/public/igem/BBa_K678033/annotation2127395/range2127395/1
type: Range
persistent identity: range2127395
display id: range2127395
version: 1
direction: direction/forward
top level: GFP-PTS1
start: 1
end: 8
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2127398
display id: annotation2127398
version: 1
top level: GFP-PTS1
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K678033/annotation2127398/range2127398/1

https://synbiohub.org/public/igem/BBa_K678033/annotation2127398/range2127398/1
type: Range
persistent identity: range2127398
display id: range2127398
version: 1
direction: direction/forward
top level: GFP-PTS1
start: 735
end: 737
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation2127396
display id: annotation2127396
version: 1
top level: GFP-PTS1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K678033/annotation2127396/range2127396/1

https://synbiohub.org/public/igem/BBa_K678033/annotation2127396/range2127396/1
type: Range
persistent identity: range2127396
display id: range2127396
version: 1
direction: direction/forward
top level: GFP-PTS1
start: 9
end: 725
orientation: inline

BBa_K900005
type: ComponentDefinition
persistent identity: BBa K900005
display id: BBa_K900005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plasma Membrane Targeting Sequence
creator: Austin Jones
created: 2012-10-01T11:00:00Z
modified: 2015-05-08T01:13:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1165_
m user id: 0
owner id: 13597
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N terminal signal sequence (CIIC) that targets the protein to the cellular periphery. The signal sequence isderived from the Ras protein, a signal protein located in the cell membrane that activates in response to extracellular signals for growth and differentiation
mutable notes: none
mutable provenance: Fused to GFP using Golden Gate cloning.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence: https://synbiohub.org/public/igem/BBa_K900005_sequence/1

BBa_K416000
type: ComponentDefinition
persistent identity: BBa K416000
display id: BBa_K416000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Aga2 CDS Responsible for Yeast Surface Display
creator: Russell Durrett
created: 2010-05-27T11:00:00Z
modified: 2015-05-08T01:12:27Z
discontinued: false
dominant: false
experience: experience/None
group u list: _527_
m user id: 0
owner id: 6443
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: The Aga2 protein is fused in frame with a protein that the user wants to display on the yeast cellular surface. Yeast cells constitutively expressing Aga1 (strain EBY100) secrete the Aga2 fusion protein, which associates with Aga1 through two disulfide bonds and quaternary structure to anchor Aga2 to the surface. The fused protein then is displayed on the surface of the yeast cell. The STOP codon at the end of the CDS was removed to allow for N- and C-terminus fusions.
mutable notes: We removed the STOP codon to allow for N- and C-terminal fusions, which have both been proven to occur efficiently.
mutable provenance: This parts was biobricked from the pCTCON2 plasmid which was sent to us by Dr. D. K. Wittrup at MIT.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence annotation: Aga2 CDS
sequence: https://synbiohub.org/public/igem/BBa_K416000_sequence/1

Aga2 CDS
type: SequenceAnnotation
persistent identity: annotation2069499
display id: annotation2069499
version: 1
top level: BBa_K416000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K416000/annotation2069499/range2069499/1

https://synbiohub.org/public/igem/BBa_K416000/annotation2069499/range2069499/1
type: Range
persistent identity: range2069499
display id: range2069499
version: 1
direction: direction/forward
top level: BBa_K416000
start: 1
end: 261
orientation: inline

OmpA
type: ComponentDefinition
persistent identity: BBa K103006
display id: BBa_K103006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: OmpA outer membrane protein A fused to linker; displays proteins on cell surface
creator: Michael Lower
created: 2008-09-13T11:00:00Z
modified: 2015-05-08T01:08:46Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _180_
m user id: 0
owner id: 2582
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: One of our basic bricks used to create fusions attached to outer membrane
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence annotation: linker
sequence annotation: ATG
sequence annotation: NdeI
sequence annotation: SacI
sequence annotation: OmpA
sequence: https://synbiohub.org/public/igem/BBa_K103006_sequence/1

linker
type: SequenceAnnotation
persistent identity: annotation1990001
display id: annotation1990001
version: 1
top level: OmpA
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K103006/annotation1990001/range1990001/1

https://synbiohub.org/public/igem/BBa_K103006/annotation1990001/range1990001/1
type: Range
persistent identity: range1990001
display id: range1990001
version: 1
direction: direction/forward
top level: OmpA
start: 439
end: 464
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation1993480
display id: annotation1993480
version: 1
top level: OmpA
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K103006/annotation1993480/range1993480/1

https://synbiohub.org/public/igem/BBa_K103006/annotation1993480/range1993480/1
type: Range
persistent identity: range1993480
display id: range1993480
version: 1
direction: direction/forward
top level: OmpA
start: 4
end: 6
orientation: inline

NdeI
type: SequenceAnnotation
persistent identity: annotation1989999
display id: annotation1989999
version: 1
top level: OmpA
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K103006/annotation1989999/range1989999/1

https://synbiohub.org/public/igem/BBa_K103006/annotation1989999/range1989999/1
type: Range
persistent identity: range1989999
display id: range1989999
version: 1
direction: direction/forward
top level: OmpA
start: 1
end: 7
orientation: inline

SacI
type: SequenceAnnotation
persistent identity: annotation1990002
display id: annotation1990002
version: 1
top level: OmpA
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K103006/annotation1990002/range1990002/1

https://synbiohub.org/public/igem/BBa_K103006/annotation1990002/range1990002/1
type: Range
persistent identity: range1990002
display id: range1990002
version: 1
direction: direction/forward
top level: OmpA
start: 459
end: 464
orientation: inline

OmpA
type: SequenceAnnotation
persistent identity: annotation1990000
display id: annotation1990000
version: 1
top level: OmpA
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K103006/annotation1990000/range1990000/1

https://synbiohub.org/public/igem/BBa_K103006/annotation1990000/range1990000/1
type: Range
persistent identity: range1990000
display id: range1990000
version: 1
direction: direction/forward
top level: OmpA
start: 4
end: 438
orientation: inline

PKI NES
type: ComponentDefinition
persistent identity: BBa J63007
display id: BBa_J63007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PKI nuclear export sequence; yeast codon optimized
creator: Caroline Ajo-Franklin
created: 2006-10-10T11:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _97_
m user id: 0
owner id: 545
owning group id: 97
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: yeast codon optimized PKI nuclear export sequence
mutable notes: optimized codons for S. cerevisiae
mutable provenance: from genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence: https://synbiohub.org/public/igem/BBa_J63007_sequence/1

SicP-SptP
type: ComponentDefinition
persistent identity: BBa J64008
display id: BBa_J64008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SicP-SptP chaperone-secretion tag pair from Salmonella type III secretion system
creator: Dan Widmaier
created: 2008-01-15T12:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _98_
m user id: 0
owner id: 2407
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is the natural implementation of the SicP chaperone (BBa_J64002) and SptP secretion signal (BBa_J64003) from the Salmonella Type III Secretion System (T3S, the Salmonella T3S coding region is referred to as Salmonella Pathogeneity Island 1 [SPI-1]). In the natural implementation the coding regions of SicP and SptP overlap out of frame. This part captures that configuration for use in creating secreted protein / chaperone pairs.
mutable notes: This part is not compatible with biobrick standard assembly.
mutable provenance: Salmonella LT2 Genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence annotation: sptP RBS
sequence annotation: sicP
sequence annotation: sptP167
sequence: https://synbiohub.org/public/igem/BBa_J64008_sequence/1

sptP RBS
type: SequenceAnnotation
persistent identity: annotation1959602
display id: annotation1959602
version: 1
top level: SicP-SptP
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J64008/annotation1959602/range1959602/1

https://synbiohub.org/public/igem/BBa_J64008/annotation1959602/range1959602/1
type: Range
persistent identity: range1959602
display id: range1959602
version: 1
direction: direction/forward
top level: SicP-SptP
start: 359
end: 379
orientation: inline

sicP
type: SequenceAnnotation
persistent identity: annotation1959600
display id: annotation1959600
version: 1
top level: SicP-SptP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_J64008/annotation1959600/range1959600/1

https://synbiohub.org/public/igem/BBa_J64008/annotation1959600/range1959600/1
type: Range
persistent identity: range1959600
display id: range1959600
version: 1
direction: direction/forward
top level: SicP-SptP
start: 1
end: 393
orientation: inline

sptP167
type: SequenceAnnotation
persistent identity: annotation1959601
display id: annotation1959601
version: 1
top level: SicP-SptP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_J64008/annotation1959601/range1959601/1

https://synbiohub.org/public/igem/BBa_J64008/annotation1959601/range1959601/1
type: Range
persistent identity: range1959601
display id: range1959601
version: 1
direction: direction/forward
top level: SicP-SptP
start: 380
end: 880
orientation: inline

BBa_K283036
type: ComponentDefinition
persistent identity: BBa K283036
display id: BBa_K283036
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: invasin(1-625aa)
creator: Shi Lei
created: 2009-10-15T11:00:00Z
modified: 2015-05-08T01:11:48Z
discontinued: false
dominant: false
experience: experience/None
group u list: _383_
m user id: 0
owner id: 5494
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part includes a LacY coding sequence on the backbone J61002
mutable notes: BBa_J61002
mutable provenance: From the registry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: invasin (1-625aa)
sequence: https://synbiohub.org/public/igem/BBa_K283036_sequence/1

invasin (1-625aa)
type: SequenceAnnotation
persistent identity: annotation2047678
display id: annotation2047678
version: 1
top level: BBa_K283036
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K283036/annotation2047678/range2047678/1

https://synbiohub.org/public/igem/BBa_K283036/annotation2047678/range2047678/1
type: Range
persistent identity: range2047678
display id: range2047678
version: 1
direction: direction/forward
top level: BBa_K283036
start: 1
end: 1900
orientation: inline

MFa1-Secre
type: ComponentDefinition
persistent identity: BBa K792002
display id: BBa_K792002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Secretion tag from yeast α-factor mating pheromone (MFα1)
creator: Manuel Gim??nez
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:23Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1047_
m user id: 0
owner id: 11811
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is the secretion signal peptide for the yeast α-mating factor. This signal peptide directs the secretion of the produced protein, and therefore allows for the exportation of it. This peptides are cleaved once the protein is in the lumen of the ER.
mutable notes: None
mutable provenance: Prepro-a-factorHas a Cleavable Signal Sequence [Water et al 1987]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence annotation: secretion tag
sequence: https://synbiohub.org/public/igem/BBa_K792002_sequence/1

secretion tag
type: SequenceAnnotation
persistent identity: annotation2197873
display id: annotation2197873
version: 1
top level: MFa1-Secre
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K792002/annotation2197873/range2197873/1

https://synbiohub.org/public/igem/BBa_K792002/annotation2197873/range2197873/1
type: Range
persistent identity: range2197873
display id: range2197873
version: 1
direction: direction/forward
top level: MFa1-Secre
start: 1
end: 54
orientation: inline

BBa_K900004
type: ComponentDefinition
persistent identity: BBa K900004
display id: BBa_K900004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Actin Targeting Protein
creator: Austin Jones
created: 2012-10-01T11:00:00Z
modified: 2015-05-08T01:13:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1165_
m user id: 0
owner id: 13597
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The actin targeting protein, ABP1, maintains the actin cytoskeleton. C terminally fusing a protein of interest to ABP1 will localize it to the actin.
mutable notes: The gene was codon optimized for yeast and internal restriction sites were removed for to compatibility with our Golden Gate cloning scheme.
mutable provenance: PCR'd from the genome.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K900004_sequence/1

BBa_K1936001
type: ComponentDefinition
persistent identity: BBa K1936001
display id: BBa_K1936001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tom5-eGFP-LOV2
creator: Marcel Dickmanns
created: 2016-10-12T11:00:00Z
modified: 2016-10-19T03:03:02Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2403_
m user id: 30478
owner id: 31678
owning group id: 9
status: status/Planning
bookmark: true
mutable description: ALERT !!!Under construction !!
mutable notes: ALERT !!!Under construction !!
mutable provenance: ALERT !!!Under construction !!
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence annotation: Linker
sequence annotation: TOM5
sequence annotation: eGFP
sequence annotation: LOV2
sequence: https://synbiohub.org/public/igem/BBa_K1936001_sequence/1

Linker
type: SequenceAnnotation
persistent identity: annotation2521540
display id: annotation2521540
version: 1
top level: BBa_K1936001
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1936001/annotation2521540/range2521540/1

https://synbiohub.org/public/igem/BBa_K1936001/annotation2521540/range2521540/1
type: Range
persistent identity: range2521540
display id: range2521540
version: 1
direction: direction/forward
top level: BBa_K1936001
start: 907
end: 936
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2521538
display id: annotation2521538
version: 1
top level: BBa_K1936001
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1936001/annotation2521538/range2521538/1

https://synbiohub.org/public/igem/BBa_K1936001/annotation2521538/range2521538/1
type: Range
persistent identity: range2521538
display id: range2521538
version: 1
direction: direction/forward
top level: BBa_K1936001
start: 154
end: 192
orientation: inline

TOM5
type: SequenceAnnotation
persistent identity: annotation2521537
display id: annotation2521537
version: 1
top level: BBa_K1936001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1936001/annotation2521537/range2521537/1

https://synbiohub.org/public/igem/BBa_K1936001/annotation2521537/range2521537/1
type: Range
persistent identity: range2521537
display id: range2521537
version: 1
direction: direction/forward
top level: BBa_K1936001
start: 1
end: 153
orientation: inline

eGFP
type: SequenceAnnotation
persistent identity: annotation2521539
display id: annotation2521539
version: 1
top level: BBa_K1936001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1936001/annotation2521539/range2521539/1

https://synbiohub.org/public/igem/BBa_K1936001/annotation2521539/range2521539/1
type: Range
persistent identity: range2521539
display id: range2521539
version: 1
direction: direction/forward
top level: BBa_K1936001
start: 193
end: 906
orientation: inline

LOV2
type: SequenceAnnotation
persistent identity: annotation2521541
display id: annotation2521541
version: 1
top level: BBa_K1936001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1936001/annotation2521541/range2521541/1

https://synbiohub.org/public/igem/BBa_K1936001/annotation2521541/range2521541/1
type: Range
persistent identity: range2521541
display id: range2521541
version: 1
direction: direction/forward
top level: BBa_K1936001
start: 937
end: 1368
orientation: inline

BBa_K157010
type: ComponentDefinition
persistent identity: BBa K157010
display id: BBa_K157010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: glycine-serine linker fused to B-cell receptor transmembrane region; displays protein on cell surfac
creator: iGEM Team Freiburg 2008
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Helical single-span transmembrane region of the B-Cell-Receptor with a flexible 15 aa Linker at the N-terminus. Designed for fusion to proteins or peptides that are to be presented at the cells surface; signal peptide for membrane integration (e.g. part Bba_K157001) required at the N-terminus of the whole construct!
mutable notes: Between BioBrick 1.0 sites this part is flanked 5' by NgoMI(=NgoMIV) and 3' AgeI(=PinAI) to facilitate in frame cloning for protein fusions.
mutable provenance: Gene synthesis by GeneArt, optimized for expression in homo sapiens.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: GGGGSx3-BCR-Transmembrane
sequence: https://synbiohub.org/public/igem/BBa_K157010_sequence/1

GGGGSx3-BCR-Transmembrane
type: SequenceAnnotation
persistent identity: annotation2064765
display id: annotation2064765
version: 1
top level: BBa_K157010
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157010/annotation2064765/range2064765/1

https://synbiohub.org/public/igem/BBa_K157010/annotation2064765/range2064765/1
type: Range
persistent identity: range2064765
display id: range2064765
version: 1
direction: direction/forward
top level: BBa_K157010
start: 1
end: 108
orientation: inline

BBa_K416004
type: ComponentDefinition
persistent identity: BBa K416004
display id: BBa_K416004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Aga2:(Gly3Ser)4 Linker Composite Part
creator: Russell Durrett
created: 2010-07-18T11:00:00Z
modified: 2015-05-08T01:12:27Z
discontinued: false
dominant: false
group u list: _527_
m user id: 0
owner id: 6443
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This composite part consists of the Aga2 yeast surface display CDS linked to a flexible (Glycine3Ser)4 linker. It can be used by linking it upstream of the protein you wish to be displayed on the surface of the yeast cell. Note: Aga1 must be constitutively expressed by the yeast strain for Aga2 to anchor to the cellular surface.
mutable notes: This composite part was put together following RFC23, thus the scar between parts consists of the sequence ACTAGA
mutable provenance: This part comes from the Aga2 [K416000] and Linker [K416001] parts we constructed this year.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K416000
component: BBa_K416001
sequence annotation: BBa_K416001
sequence annotation: BBa_K416000
sequence: https://synbiohub.org/public/igem/BBa_K416004_sequence/1

BBa_K416000
type: Component
persistent identity: component2074507
display id: component2074507
version: 1
top level: BBa_K416004
access: public
definition: BBa_K416000

BBa_K416001
type: Component
persistent identity: component2074508
display id: component2074508
version: 1
top level: BBa_K416004
access: public
definition: https://synbiohub.org/public/igem/BBa_K416001/1

BBa_K416001
type: SequenceAnnotation
persistent identity: annotation2074508
display id: annotation2074508
version: 1
top level: BBa_K416004
location: https://synbiohub.org/public/igem/BBa_K416004/annotation2074508/range2074508/1
component: BBa_K416001

https://synbiohub.org/public/igem/BBa_K416004/annotation2074508/range2074508/1
type: Range
persistent identity: range2074508
display id: range2074508
version: 1
direction: direction/reverse
top level: BBa_K416004
start: 270
end: 314
orientation: inline

BBa_K416000
type: SequenceAnnotation
persistent identity: annotation2074507
display id: annotation2074507
version: 1
top level: BBa_K416004
location: https://synbiohub.org/public/igem/BBa_K416004/annotation2074507/range2074507/1
component: BBa_K416000

https://synbiohub.org/public/igem/BBa_K416004/annotation2074507/range2074507/1
type: Range
persistent identity: range2074507
display id: range2074507
version: 1
direction: direction/reverse
top level: BBa_K416004
start: 1
end: 261
orientation: inline

PelB
type: ComponentDefinition
persistent identity: BBa J32015
display id: BBa_J32015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PelB leader sequence; directs protein to E. coli periplasmic membrane
creator: Austen Heinz
created: 2006-08-24T11:00:00Z
modified: 2015-08-31T04:08:46Z
discontinued: false
dominant: true
experience: experience/None
group u list: _50_
m user id: 0
owner id: 495
owning group id: 50
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: The pelB leader sequence is a sequence of amino acids which when attached to a protein, directs the protein to the periplasmic membrane of E. coli, where the sequence is removed by pelB peptidase. It is used to direct coat protein-antigen fusions to the cell surface.
mutable notes: .
mutable provenance: Novagen
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence annotation: PelB
sequence: https://synbiohub.org/public/igem/BBa_J32015_sequence/1

PelB
type: SequenceAnnotation
persistent identity: annotation1898182
display id: annotation1898182
version: 1
top level: PelB
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_J32015/annotation1898182/range1898182/1

https://synbiohub.org/public/igem/BBa_J32015/annotation1898182/range1898182/1
type: Range
persistent identity: range1898182
display id: range1898182
version: 1
direction: direction/forward
top level: PelB
start: 1
end: 66
orientation: inline

BBa_K1680004
type: ComponentDefinition
persistent identity: BBa K1680004
display id: BBa_K1680004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SV40 NLS
creator: Katharina Sporbeck
created: 2015-09-16T11:00:00Z
modified: 2015-10-28T06:41:10Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2098_
m user id: 26366
owner id: 26460
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Protein coding sequence for nuclear localization signal of SV40 large T antigen (amino acids: PKKKRKV).
mutable notes: Part is shipped in RFC25.
mutable provenance: gBlock designed by Team Tuebingen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence: https://synbiohub.org/public/igem/BBa_K1680004_sequence/1

BBa_K416003
type: ComponentDefinition
persistent identity: BBa K416003
display id: BBa_K416003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Yeast Secretion Tag
creator: John Phair
created: 2010-06-22T11:00:00Z
modified: 2015-05-08T01:12:27Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _527_
m user id: 0
owner id: 7430
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This secretion tag when linked to the N-terminal of the protein directs extracellular secretion of the protein. After targeting through the ER to the golgi, this "pre-pro" tag is cleaved by the KEX-2 protease resulting in secretion of protein without the aforementioned tag. To use this tag, attached it directly upstream of your proteins CDS.
mutable notes: There was an illegal Xbal1 site at bp 101 and we removed it through altering the primers necessary for biobricking the sequence.
mutable provenance: This part was sent to use from Dr. Sheldon Park of the University of Buffalo.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence: https://synbiohub.org/public/igem/BBa_K416003_sequence/1

ErbB1 (SP)
type: ComponentDefinition
persistent identity: BBa K157001
display id: BBa_K157001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: EGFR/ErbB1 signal peptide; mediates protein transport to a translocational pore
creator: iGEM Team Freiburg 2008
created: 2008-10-23T11:00:00Z
modified: 2015-05-08T01:10:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The N-terminal signal-peptide ensures protein transport to the cytoplasm-membrane.
mutable notes: none
mutable provenance: Part derived from the sequence of EGFR/ErbB1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: SP
sequence annotation: ErbB-1 Signal peptide
sequence: https://synbiohub.org/public/igem/BBa_K157001_sequence/1

SP
type: SequenceAnnotation
persistent identity: annotation2041667
display id: annotation2041667
version: 1
top level: ErbB1 (SP)
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157001/annotation2041667/range2041667/1

https://synbiohub.org/public/igem/BBa_K157001/annotation2041667/range2041667/1
type: Range
persistent identity: range2041667
display id: range2041667
version: 1
direction: direction/forward
top level: ErbB1 (SP)
start: 1
end: 72
orientation: inline

ErbB-1 Signal peptide
type: SequenceAnnotation
persistent identity: annotation2041666
display id: annotation2041666
version: 1
top level: ErbB1 (SP)
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157001/annotation2041666/range2041666/1

https://synbiohub.org/public/igem/BBa_K157001/annotation2041666/range2041666/1
type: Range
persistent identity: range2041666
display id: range2041666
version: 1
direction: direction/forward
top level: ErbB1 (SP)
start: 1
end: 72
orientation: inline

BBa_K900002
type: ComponentDefinition
persistent identity: BBa K900002
display id: BBa_K900002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Nucleus Targeting Protein
creator: Austin Jones
created: 2012-10-01T11:00:00Z
modified: 2015-05-08T01:13:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1165_
m user id: 0
owner id: 13597
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: long description
mutable notes: modified for expression in yeast.
mutable provenance: PCR'd from the genome.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K900002_sequence/1

BBa_K801043
type: ComponentDefinition
persistent identity: BBa K801043
display id: BBa_K801043
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LexA based yeast light-swithable promoter system
creator: Dong-Jiunn Jeffery TRUONG
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1057_
m user id: 0
owner id: 11507
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: composite part of Bba_K319003, K801039, BBa_K801011 , Bba_K319003, Bba_K801041, and BBa_K801011
mutable notes: -
mutable provenance: composite part of Bba_K319003, K801039, BBa_K801011 , Bba_K319003, Bba_K801041, and BBa_K801011
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: Phytochrome B (PhyB, 908 first N-terminal amino acids)
sequence annotation: BBa_K801039 (SV40NLS-GAL4AD-Linker-PIF3)
sequence annotation: T7 promoter (for sequencing)
sequence annotation: BBa_K801041 (SV40NLS-PhyB-Linker-LexA)
sequence annotation: BBa_K801011, (tTEF1, TEF1 terminator)
sequence annotation: PIF3 (First 100 N-terminal amino acids)
sequence annotation: GAL4AD (GAL4 transcriptonal activation domain)
sequence annotation: LexA
sequence annotation: BBa_K319003 (pTEF1, TEF1 promoter)
sequence annotation: SV40 NLS (SV40 nuclear localization signal)
sequence annotation: Linker
sequence annotation: BBa_K365005 (20 amino acids long linker)
sequence annotation: SV40NLS-PhyB-Linker-LexA
sequence annotation: HA epitope tage
sequence: https://synbiohub.org/public/igem/BBa_K801043_sequence/1

Phytochrome B (PhyB, 908 first N-terminal amino acids)
type: SequenceAnnotation
persistent identity: annotation2198809
display id: annotation2198809
version: 1
top level: BBa_K801043
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K801043/annotation2198809/range2198809/1

https://synbiohub.org/public/igem/BBa_K801043/annotation2198809/range2198809/1
type: Range
persistent identity: range2198809
display id: range2198809
version: 1
direction: direction/forward
top level: BBa_K801043
start: 2309
end: 5029
orientation: inline

BBa_K801039 (SV40NLS-GAL4AD-Linker-PIF3)
type: SequenceAnnotation
persistent identity: annotation2198798
display id: annotation2198798
version: 1
top level: BBa_K801043
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801043/annotation2198798/range2198798/1

https://synbiohub.org/public/igem/BBa_K801043/annotation2198798/range2198798/1
type: Range
persistent identity: range2198798
display id: range2198798
version: 1
direction: direction/forward
top level: BBa_K801043
start: 456
end: 1259
orientation: inline

T7 promoter (for sequencing)
type: SequenceAnnotation
persistent identity: annotation2198802
display id: annotation2198802
version: 1
top level: BBa_K801043
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K801043/annotation2198802/range2198802/1

https://synbiohub.org/public/igem/BBa_K801043/annotation2198802/range2198802/1
type: Range
persistent identity: range2198802
display id: range2198802
version: 1
direction: direction/forward
top level: BBa_K801043
start: 869
end: 891
orientation: inline

BBa_K801041 (SV40NLS-PhyB-Linker-LexA)
type: SequenceAnnotation
persistent identity: annotation2198814
display id: annotation2198814
version: 1
top level: BBa_K801043
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801043/annotation2198814/range2198814/1

https://synbiohub.org/public/igem/BBa_K801043/annotation2198814/range2198814/1
type: Range
persistent identity: range2198814
display id: range2198814
version: 1
direction: direction/forward
top level: BBa_K801043
start: 2246
end: 5704
orientation: inline

BBa_K801011, (tTEF1, TEF1 terminator)
type: SequenceAnnotation
persistent identity: annotation2198805
display id: annotation2198805
version: 1
top level: BBa_K801043
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801043/annotation2198805/range2198805/1

https://synbiohub.org/public/igem/BBa_K801043/annotation2198805/range2198805/1
type: Range
persistent identity: range2198805
display id: range2198805
version: 1
direction: direction/forward
top level: BBa_K801043
start: 1268
end: 1774
orientation: inline

PIF3 (First 100 N-terminal amino acids)
type: SequenceAnnotation
persistent identity: annotation2198804
display id: annotation2198804
version: 1
top level: BBa_K801043
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K801043/annotation2198804/range2198804/1

https://synbiohub.org/public/igem/BBa_K801043/annotation2198804/range2198804/1
type: Range
persistent identity: range2198804
display id: range2198804
version: 1
direction: direction/forward
top level: BBa_K801043
start: 960
end: 1256
orientation: inline

GAL4AD (GAL4 transcriptonal activation domain)
type: SequenceAnnotation
persistent identity: annotation2198800
display id: annotation2198800
version: 1
top level: BBa_K801043
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K801043/annotation2198800/range2198800/1

https://synbiohub.org/public/igem/BBa_K801043/annotation2198800/range2198800/1
type: Range
persistent identity: range2198800
display id: range2198800
version: 1
direction: direction/forward
top level: BBa_K801043
start: 525
end: 863
orientation: inline

LexA
type: SequenceAnnotation
persistent identity: annotation2198811
display id: annotation2198811
version: 1
top level: BBa_K801043
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K801043/annotation2198811/range2198811/1

https://synbiohub.org/public/igem/BBa_K801043/annotation2198811/range2198811/1
type: Range
persistent identity: range2198811
display id: range2198811
version: 1
direction: direction/forward
top level: BBa_K801043
start: 5102
end: 5704
orientation: inline

BBa_K319003 (pTEF1, TEF1 promoter)
type: SequenceAnnotation
persistent identity: annotation2198806
display id: annotation2198806
version: 1
top level: BBa_K801043
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K801043/annotation2198806/range2198806/1

https://synbiohub.org/public/igem/BBa_K801043/annotation2198806/range2198806/1
type: Range
persistent identity: range2198806
display id: range2198806
version: 1
direction: direction/forward
top level: BBa_K801043
start: 1782
end: 2231
orientation: inline

BBa_K801011, (tTEF1, TEF1 terminator)
type: SequenceAnnotation
persistent identity: annotation2198812
display id: annotation2198812
version: 1
top level: BBa_K801043
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801043/annotation2198812/range2198812/1

https://synbiohub.org/public/igem/BBa_K801043/annotation2198812/range2198812/1
type: Range
persistent identity: range2198812
display id: range2198812
version: 1
direction: direction/forward
top level: BBa_K801043
start: 5722
end: 6228
orientation: inline

SV40 NLS (SV40 nuclear localization signal)
type: SequenceAnnotation
persistent identity: annotation2198799
display id: annotation2198799
version: 1
top level: BBa_K801043
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801043/annotation2198799/range2198799/1

https://synbiohub.org/public/igem/BBa_K801043/annotation2198799/range2198799/1
type: Range
persistent identity: range2198799
display id: range2198799
version: 1
direction: direction/forward
top level: BBa_K801043
start: 465
end: 521
orientation: inline

BBa_K319003 (pTEF1, TEF1 promoter)
type: SequenceAnnotation
persistent identity: annotation2198797
display id: annotation2198797
version: 1
top level: BBa_K801043
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K801043/annotation2198797/range2198797/1

https://synbiohub.org/public/igem/BBa_K801043/annotation2198797/range2198797/1
type: Range
persistent identity: range2198797
display id: range2198797
version: 1
direction: direction/forward
top level: BBa_K801043
start: 1
end: 450
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2198801
display id: annotation2198801
version: 1
top level: BBa_K801043
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801043/annotation2198801/range2198801/1

https://synbiohub.org/public/igem/BBa_K801043/annotation2198801/range2198801/1
type: Range
persistent identity: range2198801
display id: range2198801
version: 1
direction: direction/forward
top level: BBa_K801043
start: 864
end: 959
orientation: inline

BBa_K365005 (20 amino acids long linker)
type: SequenceAnnotation
persistent identity: annotation2198810
display id: annotation2198810
version: 1
top level: BBa_K801043
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801043/annotation2198810/range2198810/1

https://synbiohub.org/public/igem/BBa_K801043/annotation2198810/range2198810/1
type: Range
persistent identity: range2198810
display id: range2198810
version: 1
direction: direction/forward
top level: BBa_K801043
start: 5036
end: 5095
orientation: inline

SV40NLS-PhyB-Linker-LexA
type: SequenceAnnotation
persistent identity: annotation2198807
display id: annotation2198807
version: 1
top level: BBa_K801043
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801043/annotation2198807/range2198807/1

https://synbiohub.org/public/igem/BBa_K801043/annotation2198807/range2198807/1
type: Range
persistent identity: range2198807
display id: range2198807
version: 1
direction: direction/forward
top level: BBa_K801043
start: 2237
end: 5713
orientation: inline

HA epitope tage
type: SequenceAnnotation
persistent identity: annotation2198803
display id: annotation2198803
version: 1
top level: BBa_K801043
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801043/annotation2198803/range2198803/1

https://synbiohub.org/public/igem/BBa_K801043/annotation2198803/range2198803/1
type: Range
persistent identity: range2198803
display id: range2198803
version: 1
direction: direction/forward
top level: BBa_K801043
start: 912
end: 932
orientation: inline

SV40 NLS (SV40 nuclear localization signal)
type: SequenceAnnotation
persistent identity: annotation2198808
display id: annotation2198808
version: 1
top level: BBa_K801043
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801043/annotation2198808/range2198808/1

https://synbiohub.org/public/igem/BBa_K801043/annotation2198808/range2198808/1
type: Range
persistent identity: range2198808
display id: range2198808
version: 1
direction: direction/forward
top level: BBa_K801043
start: 2246
end: 2302
orientation: inline

BBa_I712016
type: ComponentDefinition
persistent identity: BBa I712016
display id: BBa_I712016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: myristoilation signal sequence fused to Tobacco Etch Virus (TEV) protease cleavage site
creator: Katja Kolar
created: 2007-10-22T11:00:00Z
modified: 2015-08-31T04:07:46Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _130_
m user id: 0
owner id: 1883
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Signal sequence for myristoilation with proteolytic cleavage site for TEV protease.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence: https://synbiohub.org/public/igem/BBa_I712016_sequence/1

BBa_K249005
type: ComponentDefinition
persistent identity: BBa K249005
display id: BBa_K249005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: C-terminal Arginine Fusion Vector
creator: Roxanne Shank
created: 2009-08-25T11:00:00Z
modified: 2015-05-08T01:11:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _342_
m user id: 0
owner id: 3171
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: pSB1A3 with an C-terminal Arginine tag. This particular plasmid utilizes the BioFusion (aka Silver Lab) Standard for BioBrick assembly. Adds 10 Arginine residues to the C-terminus of a Protein (Includes Stop Codon)
mutable notes: Standard
mutable provenance: Synthesized DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K249005_sequence/1

BBa_K249004
type: ComponentDefinition
persistent identity: BBa K249004
display id: BBa_K249004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: N-terminal Arginine fusion vector
creator: Roxanne Shank
created: 2009-08-25T11:00:00Z
modified: 2015-05-08T01:11:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _342_
m user id: 0
owner id: 3171
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: pSB1A3 with an N-terminal Arginine tag. This particular plasmid utilizes the BioFusion (aka Silver Lab) Standard for BioBrick assembly. Adds 10 Arginine residues to the N-terminus of a Protein.
mutable notes: Standard
mutable provenance: Synthesized Tag
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K249004_sequence/1

SV40 NLS
type: ComponentDefinition
persistent identity: BBa J63008
display id: BBa_J63008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SV40 nuclear localization sequence from SV40; yeast codon optimized
creator: Caroline Ajo-Franklin
created: 2006-10-10T11:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _97_
m user id: 0
owner id: 545
owning group id: 97
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: yeast codon-optimized nuclear localization sequence from simian virus 40
mutable notes: as described in literature
mutable provenance: direct synthesis from published sequence
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence: https://synbiohub.org/public/igem/BBa_J63008_sequence/1

CD4 signal
type: ComponentDefinition
persistent identity: BBa I712009
display id: BBa_I712009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CD4 signal peptide; localizes to plasma membrane
creator: Peter Cimermancic
created: 2007-10-21T11:00:00Z
modified: 2015-08-31T04:07:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _130_
m user id: 0
owner id: 1850
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Coding region for first 25 amino acids (including start codon) of CD4 receptor which is signal peptide for plasma membrane localization.
mutable notes: No considerations.
mutable provenance: It comes from human gene for CD4 receptor.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence: https://synbiohub.org/public/igem/BBa_I712009_sequence/1

BBa_K801030
type: ComponentDefinition
persistent identity: BBa K801030
display id: BBa_K801030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Polypeptide containing SV40 nuclear localization sequence (SV40 NLS) for nuclear translocation
creator: Dong-Jiunn Jeffery TRUONG
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1057_
m user id: 0
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: SV40NLS nuclear localization sequence
mutable notes: -
mutable provenance: designed by oligo hybridisation based on plasmid "pGADT7 AD" from Clontech, w/ RFC25-pre and suffix for protein fusions
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: SV40 nuclear localization sequence (recognized by importin alpha)
sequence: https://synbiohub.org/public/igem/BBa_K801030_sequence/1

SV40 nuclear localization sequence (recognized by importin alpha)
type: SequenceAnnotation
persistent identity: annotation2198843
display id: annotation2198843
version: 1
top level: BBa_K801030
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801030/annotation2198843/range2198843/1

https://synbiohub.org/public/igem/BBa_K801030/annotation2198843/range2198843/1
type: Range
persistent identity: range2198843
display id: range2198843
version: 1
direction: direction/forward
top level: BBa_K801030
start: 22
end: 42
orientation: inline

secretion
type: ComponentDefinition
persistent identity: BBa K128004
display id: BBa_K128004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: signal sequence from Lactobacillus bulgaricus; secretes protein
creator: Sara Mouradian
created: 2008-07-17T11:00:00Z
modified: 2015-05-08T01:09:46Z
discontinued: false
dominant: false
experience: experience/None
group u list: _178_
m user id: 0
owner id: 2822
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a short signal sequence recognized as a secretion tag by several Lactobacillus strains. As the protein that is attatched to is secreted, the signal peptide is cut off. Using SignalP, the cleavage site is between residues 39 and 40 (VQA - AS). This may change depending on the following sequence. This part uses the modified Silver BioBirck prefix and suffix to allow for protein construction.
mutable notes: The cleavage site may be influenced by the protein that is attached to the signal peptide. To make sure that the protein does not get cleaved by mistake, four residues are added to the signal peptide sequence found by the Germond paper. Signal peptides are almost always used in conjunction with another protein so we used the modified Silver BioBrick prefix and suffix to make the construction of funcitonal fusion peptides possible
mutable provenance: It was isolated from the 5' end of the prtB gene in Lactobacillus bulgaricus, which codes for a lactose digesting protein that is bound to the surface (Germond JE et al.; Appl Environ Microbiol. 2003). This part uses the modified Silver BioBrick prefix and suffix to allow for protein construction.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence: https://synbiohub.org/public/igem/BBa_K128004_sequence/1

BBa_K1680013
type: ComponentDefinition
persistent identity: BBa K1680013
display id: BBa_K1680013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NDegron
creator: Katharina Sporbeck
created: 2015-09-16T11:00:00Z
modified: 2015-10-27T01:42:39Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2098_
m user id: 15693
owner id: 26460
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Protein coding region for the firefly [Photinus pyralis] luciferase, codon optimized for yeast.
mutable notes: Shipped in RFC10
mutable provenance: gBlock designed by Team Tuebingen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Nuclear export sequence
sequence annotation: SV40 NLS
sequence annotation: TEV cleavage sites
sequence annotation: Spacer
sequence annotation: NDegron
sequence: https://synbiohub.org/public/igem/BBa_K1680013_sequence/1

Nuclear export sequence
type: SequenceAnnotation
persistent identity: annotation2477351
display id: annotation2477351
version: 1
top level: BBa_K1680013
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1680013/annotation2477351/range2477351/1

https://synbiohub.org/public/igem/BBa_K1680013/annotation2477351/range2477351/1
type: Range
persistent identity: range2477351
display id: range2477351
version: 1
direction: direction/forward
top level: BBa_K1680013
start: 130
end: 147
orientation: inline

SV40 NLS
type: SequenceAnnotation
persistent identity: annotation2477348
display id: annotation2477348
version: 1
top level: BBa_K1680013
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1680013/annotation2477348/range2477348/1

https://synbiohub.org/public/igem/BBa_K1680013/annotation2477348/range2477348/1
type: Range
persistent identity: range2477348
display id: range2477348
version: 1
direction: direction/forward
top level: BBa_K1680013
start: 13
end: 33
orientation: inline

TEV cleavage sites
type: SequenceAnnotation
persistent identity: annotation2477349
display id: annotation2477349
version: 1
top level: BBa_K1680013
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1680013/annotation2477349/range2477349/1

https://synbiohub.org/public/igem/BBa_K1680013/annotation2477349/range2477349/1
type: Range
persistent identity: range2477349
display id: range2477349
version: 1
direction: direction/forward
top level: BBa_K1680013
start: 67
end: 86
orientation: inline

Spacer
type: SequenceAnnotation
persistent identity: annotation2477352
display id: annotation2477352
version: 1
top level: BBa_K1680013
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1680013/annotation2477352/range2477352/1

https://synbiohub.org/public/igem/BBa_K1680013/annotation2477352/range2477352/1
type: Range
persistent identity: range2477352
display id: range2477352
version: 1
direction: direction/forward
top level: BBa_K1680013
start: 34
end: 66
orientation: inline

NDegron
type: SequenceAnnotation
persistent identity: annotation2477347
display id: annotation2477347
version: 1
top level: BBa_K1680013
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680013/annotation2477347/range2477347/1

https://synbiohub.org/public/igem/BBa_K1680013/annotation2477347/range2477347/1
type: Range
persistent identity: range2477347
display id: range2477347
version: 1
direction: direction/forward
top level: BBa_K1680013
start: 1
end: 165
orientation: inline

NDegron
type: SequenceAnnotation
persistent identity: annotation2477350
display id: annotation2477350
version: 1
top level: BBa_K1680013
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1680013/annotation2477350/range2477350/1

https://synbiohub.org/public/igem/BBa_K1680013/annotation2477350/range2477350/1
type: Range
persistent identity: range2477350
display id: range2477350
version: 1
direction: direction/forward
top level: BBa_K1680013
start: 97
end: 138
orientation: inline

rbs.pelB
type: ComponentDefinition
persistent identity: BBa K112230
display id: BBa_K112230
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {rbs.pelB} The pelB leader with native rbs; assembly standard 21
creator: Molly Allen
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:15Z
discontinued: false
dominant: true
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2048
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: ?
mutable provenance: Synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K112230_sequence/1

BBa_K801042
type: ComponentDefinition
persistent identity: BBa K801042
display id: BBa_K801042
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GAL4 based yeast light-switchable promoter system
creator: Dong-Jiunn Jeffery TRUONG
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1057_
m user id: 0
owner id: 11507
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: composite part of Bba_K319003, BBa_K801039, BBa_K801011 Bba_K319003, Bba_K801040, and BBa_K801011
mutable notes: -
mutable provenance: composite part of Bba_K319003, BBa_K801039, BBa_K801011 Bba_K319003, Bba_K801040, and BBa_K801011
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: SV40 NLS (SV40 nuclear localization signal)
sequence annotation: BBa_K801011, (tTEF1, TEF1 terminator)
sequence annotation: Phytochrome B (PhyB, 908 first N-terminal amino acids)
sequence annotation: HA epitope tage
sequence annotation: GAL4DBD (GAL4 DNA binding domain)
sequence annotation: Linker
sequence annotation: SV40NLS-PhyB-Linker-GAL4DBD
sequence annotation: T7 promoter (for sequencing)
sequence annotation: PIF3 (First 100 N-terminal amino acids)
sequence annotation: BBa_K319003 (pTEF1, TEF1 promoter)
sequence annotation: GAL4AD (GAL4 transcriptonal activation domain)
sequence annotation: BBa_K801040 (SV40NLS-PhyB-Linker-GAL4DBD)
sequence annotation: BBa_K801039 (SV40NLS-GAL4AD-Linker-PIF3)
sequence annotation: 20 amino acids long linker (BBa_K365005)
sequence: https://synbiohub.org/public/igem/BBa_K801042_sequence/1

SV40 NLS (SV40 nuclear localization signal)
type: SequenceAnnotation
persistent identity: annotation2198792
display id: annotation2198792
version: 1
top level: BBa_K801042
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198792/range2198792/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198792/range2198792/1
type: Range
persistent identity: range2198792
display id: range2198792
version: 1
direction: direction/forward
top level: BBa_K801042
start: 2246
end: 2302
orientation: inline

BBa_K801011, (tTEF1, TEF1 terminator)
type: SequenceAnnotation
persistent identity: annotation2198816
display id: annotation2198816
version: 1
top level: BBa_K801042
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198816/range2198816/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198816/range2198816/1
type: Range
persistent identity: range2198816
display id: range2198816
version: 1
direction: direction/forward
top level: BBa_K801042
start: 5557
end: 6063
orientation: inline

Phytochrome B (PhyB, 908 first N-terminal amino acids)
type: SequenceAnnotation
persistent identity: annotation2198794
display id: annotation2198794
version: 1
top level: BBa_K801042
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198794/range2198794/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198794/range2198794/1
type: Range
persistent identity: range2198794
display id: range2198794
version: 1
direction: direction/forward
top level: BBa_K801042
start: 2309
end: 5029
orientation: inline

HA epitope tage
type: SequenceAnnotation
persistent identity: annotation2198787
display id: annotation2198787
version: 1
top level: BBa_K801042
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198787/range2198787/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198787/range2198787/1
type: Range
persistent identity: range2198787
display id: range2198787
version: 1
direction: direction/forward
top level: BBa_K801042
start: 912
end: 932
orientation: inline

GAL4DBD (GAL4 DNA binding domain)
type: SequenceAnnotation
persistent identity: annotation2198796
display id: annotation2198796
version: 1
top level: BBa_K801042
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198796/range2198796/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198796/range2198796/1
type: Range
persistent identity: range2198796
display id: range2198796
version: 1
direction: direction/forward
top level: BBa_K801042
start: 5102
end: 5539
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2198785
display id: annotation2198785
version: 1
top level: BBa_K801042
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198785/range2198785/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198785/range2198785/1
type: Range
persistent identity: range2198785
display id: range2198785
version: 1
direction: direction/forward
top level: BBa_K801042
start: 864
end: 959
orientation: inline

SV40NLS-PhyB-Linker-GAL4DBD
type: SequenceAnnotation
persistent identity: annotation2198791
display id: annotation2198791
version: 1
top level: BBa_K801042
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198791/range2198791/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198791/range2198791/1
type: Range
persistent identity: range2198791
display id: range2198791
version: 1
direction: direction/forward
top level: BBa_K801042
start: 2237
end: 5548
orientation: inline

T7 promoter (for sequencing)
type: SequenceAnnotation
persistent identity: annotation2198786
display id: annotation2198786
version: 1
top level: BBa_K801042
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198786/range2198786/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198786/range2198786/1
type: Range
persistent identity: range2198786
display id: range2198786
version: 1
direction: direction/forward
top level: BBa_K801042
start: 869
end: 891
orientation: inline

BBa_K801011, (tTEF1, TEF1 terminator)
type: SequenceAnnotation
persistent identity: annotation2198789
display id: annotation2198789
version: 1
top level: BBa_K801042
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198789/range2198789/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198789/range2198789/1
type: Range
persistent identity: range2198789
display id: range2198789
version: 1
direction: direction/forward
top level: BBa_K801042
start: 1268
end: 1774
orientation: inline

PIF3 (First 100 N-terminal amino acids)
type: SequenceAnnotation
persistent identity: annotation2198788
display id: annotation2198788
version: 1
top level: BBa_K801042
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198788/range2198788/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198788/range2198788/1
type: Range
persistent identity: range2198788
display id: range2198788
version: 1
direction: direction/forward
top level: BBa_K801042
start: 960
end: 1256
orientation: inline

BBa_K319003 (pTEF1, TEF1 promoter)
type: SequenceAnnotation
persistent identity: annotation2198790
display id: annotation2198790
version: 1
top level: BBa_K801042
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198790/range2198790/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198790/range2198790/1
type: Range
persistent identity: range2198790
display id: range2198790
version: 1
direction: direction/forward
top level: BBa_K801042
start: 1782
end: 2231
orientation: inline

GAL4AD (GAL4 transcriptonal activation domain)
type: SequenceAnnotation
persistent identity: annotation2198784
display id: annotation2198784
version: 1
top level: BBa_K801042
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198784/range2198784/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198784/range2198784/1
type: Range
persistent identity: range2198784
display id: range2198784
version: 1
direction: direction/forward
top level: BBa_K801042
start: 525
end: 863
orientation: inline

BBa_K801040 (SV40NLS-PhyB-Linker-GAL4DBD)
type: SequenceAnnotation
persistent identity: annotation2198793
display id: annotation2198793
version: 1
top level: BBa_K801042
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198793/range2198793/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198793/range2198793/1
type: Range
persistent identity: range2198793
display id: range2198793
version: 1
direction: direction/forward
top level: BBa_K801042
start: 2246
end: 5539
orientation: inline

SV40 NLS (SV40 nuclear localization signal)
type: SequenceAnnotation
persistent identity: annotation2198783
display id: annotation2198783
version: 1
top level: BBa_K801042
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198783/range2198783/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198783/range2198783/1
type: Range
persistent identity: range2198783
display id: range2198783
version: 1
direction: direction/forward
top level: BBa_K801042
start: 465
end: 521
orientation: inline

BBa_K319003 (pTEF1, TEF1 promoter)
type: SequenceAnnotation
persistent identity: annotation2198781
display id: annotation2198781
version: 1
top level: BBa_K801042
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198781/range2198781/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198781/range2198781/1
type: Range
persistent identity: range2198781
display id: range2198781
version: 1
direction: direction/forward
top level: BBa_K801042
start: 1
end: 450
orientation: inline

BBa_K801039 (SV40NLS-GAL4AD-Linker-PIF3)
type: SequenceAnnotation
persistent identity: annotation2198782
display id: annotation2198782
version: 1
top level: BBa_K801042
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198782/range2198782/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198782/range2198782/1
type: Range
persistent identity: range2198782
display id: range2198782
version: 1
direction: direction/forward
top level: BBa_K801042
start: 456
end: 1259
orientation: inline

20 amino acids long linker (BBa_K365005)
type: SequenceAnnotation
persistent identity: annotation2198795
display id: annotation2198795
version: 1
top level: BBa_K801042
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198795/range2198795/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198795/range2198795/1
type: Range
persistent identity: range2198795
display id: range2198795
version: 1
direction: direction/forward
top level: BBa_K801042
start: 5036
end: 5095
orientation: inline