//proteindomain/linker
type: Collection
persistent identity: proteindomain linker
display id: proteindomain_linker
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_K404303
member: linker
member: MiddleLink
member: BBa_K404304
member: BBa_K1486003
member: BBa_K1680001
member: BBa_K404301
member: PLrigid
member: BBa_K404305
member: BBa_K416001
member: BBa_K1777019
member: LongLinker
member: BBa_K648006
member: BBa_K1486037
member: 5xGS
member: BBa_K1680002
member: BBa_K404300
member: GSATLinker
member: BBa_K404306
member: 3xGS
member: BBa_K1486053
member: BBa_K1777005
member: BBa_K648007
member: BBa_K648005
member: 1xGS
member: BBa_K1486004
member: BBa_K1680003
member: BBa_K243030
member: ShortLinke
member: BBa_K105012

BBa_K1486004
type: ComponentDefinition
persistent identity: BBa K1486004
display id: BBa_K1486004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: flexible linker 2x (GGGGS)
creator: iGem EPFL 2014
created: 2014-08-11T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Flexible linker used to fuse two proteins.
mutable notes: Designed considering the flexibility and separation needed between our fused proteins.
mutable provenance: Synthetic.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K1486004_sequence/1

BBa_K1486053
type: ComponentDefinition
persistent identity: BBa K1486053
display id: BBa_K1486053
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 10 aa linker
creator: EPFL iGem team 2014
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: to edit
mutable notes: to edit
mutable provenance: to edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K1486053_sequence/1

BBa_K1777005
type: ComponentDefinition
persistent identity: BBa K1777005
display id: BBa_K1777005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: flexible linker with SV40 NLS
creator: Xuhang Li
created: 2015-09-12T11:00:00Z
modified: 2015-09-19T11:42:57Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _2199_
m user id: 27798
owner id: 25645
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This is a 40-50A flexible separation for fusion domains with a SV40 NLS, and its codon is optimized for mammalian. It can be used to link different protein domains for fusion proteins locating in cell nucleus.
mutable notes: The codon is optimized for mammalian, and we insert a NLS to promote locating in cell nucleus. Besides ,we lengthen this linker to provide better separation of two domains. At last we avoid tandem repeat sequence(like GGGSPKKKRKVGGGS), which will facilitate fusing this linker to your domains via overlap PCR.
mutable provenance: This part is improved from BBa_K1486003.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: artificial GS linker
sequence annotation: natural flexible linker
sequence annotation: SV40 NLS
sequence annotation: the first linker
sequence annotation: scar from 3A assembly
sequence annotation: the second linker
sequence: https://synbiohub.org/public/igem/BBa_K1777005_sequence/1

artificial GS linker
type: SequenceAnnotation
persistent identity: annotation2476145
display id: annotation2476145
version: 1
top level: BBa_K1777005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1777005/annotation2476145/range2476145/1

https://synbiohub.org/public/igem/BBa_K1777005/annotation2476145/range2476145/1
type: Range
persistent identity: range2476145
display id: range2476145
version: 1
direction: direction/forward
top level: BBa_K1777005
start: 45
end: 59
orientation: inline

artificial GS linker
type: SequenceAnnotation
persistent identity: annotation2476147
display id: annotation2476147
version: 1
top level: BBa_K1777005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1777005/annotation2476147/range2476147/1

https://synbiohub.org/public/igem/BBa_K1777005/annotation2476147/range2476147/1
type: Range
persistent identity: range2476147
display id: range2476147
version: 1
direction: direction/forward
top level: BBa_K1777005
start: 110
end: 124
orientation: inline

natural flexible linker
type: SequenceAnnotation
persistent identity: annotation2476144
display id: annotation2476144
version: 1
top level: BBa_K1777005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1777005/annotation2476144/range2476144/1

https://synbiohub.org/public/igem/BBa_K1777005/annotation2476144/range2476144/1
type: Range
persistent identity: range2476144
display id: range2476144
version: 1
direction: direction/forward
top level: BBa_K1777005
start: 3
end: 23
orientation: inline

SV40 NLS
type: SequenceAnnotation
persistent identity: annotation2476142
display id: annotation2476142
version: 1
top level: BBa_K1777005
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1777005/annotation2476142/range2476142/1

https://synbiohub.org/public/igem/BBa_K1777005/annotation2476142/range2476142/1
type: Range
persistent identity: range2476142
display id: range2476142
version: 1
direction: direction/forward
top level: BBa_K1777005
start: 24
end: 44
orientation: inline

SV40 NLS
type: SequenceAnnotation
persistent identity: annotation2476143
display id: annotation2476143
version: 1
top level: BBa_K1777005
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1777005/annotation2476143/range2476143/1

https://synbiohub.org/public/igem/BBa_K1777005/annotation2476143/range2476143/1
type: Range
persistent identity: range2476143
display id: range2476143
version: 1
direction: direction/forward
top level: BBa_K1777005
start: 89
end: 109
orientation: inline

the first linker
type: SequenceAnnotation
persistent identity: annotation2476139
display id: annotation2476139
version: 1
top level: BBa_K1777005
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1777005/annotation2476139/range2476139/1

https://synbiohub.org/public/igem/BBa_K1777005/annotation2476139/range2476139/1
type: Range
persistent identity: range2476139
display id: range2476139
version: 1
direction: direction/forward
top level: BBa_K1777005
start: 3
end: 59
orientation: inline

scar from 3A assembly
type: SequenceAnnotation
persistent identity: annotation2476141
display id: annotation2476141
version: 1
top level: BBa_K1777005
role: feature/scar
location: https://synbiohub.org/public/igem/BBa_K1777005/annotation2476141/range2476141/1

https://synbiohub.org/public/igem/BBa_K1777005/annotation2476141/range2476141/1
type: Range
persistent identity: range2476141
display id: range2476141
version: 1
direction: direction/forward
top level: BBa_K1777005
start: 60
end: 67
orientation: inline

natural flexible linker
type: SequenceAnnotation
persistent identity: annotation2476146
display id: annotation2476146
version: 1
top level: BBa_K1777005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1777005/annotation2476146/range2476146/1

https://synbiohub.org/public/igem/BBa_K1777005/annotation2476146/range2476146/1
type: Range
persistent identity: range2476146
display id: range2476146
version: 1
direction: direction/forward
top level: BBa_K1777005
start: 68
end: 88
orientation: inline

the second linker
type: SequenceAnnotation
persistent identity: annotation2476140
display id: annotation2476140
version: 1
top level: BBa_K1777005
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1777005/annotation2476140/range2476140/1

https://synbiohub.org/public/igem/BBa_K1777005/annotation2476140/range2476140/1
type: Range
persistent identity: range2476140
display id: range2476140
version: 1
direction: direction/forward
top level: BBa_K1777005
start: 68
end: 124
orientation: inline

BBa_K243030
type: ComponentDefinition
persistent identity: BBa K243030
display id: BBa_K243030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SEG
creator: Freiburg Bioware09
created: 2009-10-19T11:00:00Z
modified: 2015-05-08T01:11:37Z
discontinued: false
dominant: true
experience: experience/None
group u list: _352_
m user id: 0
owner id: 4732
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is a linker, it can be used to connect two parts and add additional space between these parts. That can be necessary to avoid interactions between these parts. The SEG linker was create to connect our protein domains Fok_a and Fok_i to an function final construct.
mutable notes: Designed for the fusion of proteins or peptides via in frame cloning, according to RFC25
mutable provenance: Ordered by Mr.Gene.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: SEG Linker
sequence: https://synbiohub.org/public/igem/BBa_K243030_sequence/1

SEG Linker
type: SequenceAnnotation
persistent identity: annotation2055436
display id: annotation2055436
version: 1
top level: BBa_K243030
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K243030/annotation2055436/range2055436/1

https://synbiohub.org/public/igem/BBa_K243030/annotation2055436/range2055436/1
type: Range
persistent identity: range2055436
display id: range2055436
version: 1
direction: direction/forward
top level: BBa_K243030
start: 1
end: 108
orientation: inline

BBa_K404306
type: ComponentDefinition
persistent identity: BBa K404306
display id: BBa_K404306
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Z-EGFR-1907_SEG-Linker
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: SEG-linker
sequence annotation: Z-EGFR-1907
sequence: https://synbiohub.org/public/igem/BBa_K404306_sequence/1

SEG-linker
type: SequenceAnnotation
persistent identity: annotation2094758
display id: annotation2094758
version: 1
top level: BBa_K404306
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404306/annotation2094758/range2094758/1

https://synbiohub.org/public/igem/BBa_K404306/annotation2094758/range2094758/1
type: Range
persistent identity: range2094758
display id: range2094758
version: 1
direction: direction/forward
top level: BBa_K404306
start: 181
end: 288
orientation: inline

Z-EGFR-1907
type: SequenceAnnotation
persistent identity: annotation2094759
display id: annotation2094759
version: 1
top level: BBa_K404306
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404306/annotation2094759/range2094759/1

https://synbiohub.org/public/igem/BBa_K404306/annotation2094759/range2094759/1
type: Range
persistent identity: range2094759
display id: range2094759
version: 1
direction: direction/forward
top level: BBa_K404306
start: 1
end: 175
orientation: inline

BBa_K1680001
type: ComponentDefinition
persistent identity: BBa K1680001
display id: BBa_K1680001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: protein linker, 9aa
creator: Katharina Sporbeck
created: 2015-09-16T11:00:00Z
modified: 2015-10-27T01:28:04Z
discontinued: false
dominant: false
group u list: _2098_
m user id: 15693
owner id: 26460
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Protein coding region for the generation of 9AA linker. The linker will be composed of 4 amino acids from the scars from using RFC25 standard and Serin/Glycin residues.
mutable notes: Parts are shipped in RFC25.
mutable provenance: gBlock designed by Team Tuebingen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K1680001_sequence/1

BBa_K1680003
type: ComponentDefinition
persistent identity: BBa K1680003
display id: BBa_K1680003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Protein linker, 15 amino acids
creator: Katharina Sporbeck
created: 2015-09-16T11:00:00Z
modified: 2015-10-27T01:28:42Z
discontinued: false
dominant: false
group u list: _2098_
m user id: 15693
owner id: 26460
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Protein coding region for the generation of 6/9/12/15AA linker. The linker will be composed of 4 amino acids from the scars from using RFC25 standard and Serin/Glycin residues.
mutable notes: Parts are shipped in RFC25.
mutable provenance: gBlock designed by Team Tuebingen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K1680003_sequence/1

MiddleLink
type: ComponentDefinition
persistent identity: BBa K243005
display id: BBa_K243005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Middle Linker ( Gly-Gly-Ser-Gly)x2
creator: Freiburg Bioware09
created: 2009-10-13T11:00:00Z
modified: 2015-05-08T01:11:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _352_
m user id: 0
owner id: 4732
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is a linker, it can be used to connect two parts and add additional space between these parts. That can be necessary to avoid interactions between these parts. We used this part to connect our protein domains with our lipocalin domains, for our project the universal endonuclease.The sequence produced the aminoacids Gly-Gly-Ser-Gly-Gly-Gly-Ser-Gly .
mutable notes: None.
mutable provenance: Oligos synthesized by sigma. Hybridized by PCR.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence annotation: MiddleLinker
sequence: https://synbiohub.org/public/igem/BBa_K243005_sequence/1

MiddleLinker
type: SequenceAnnotation
persistent identity: annotation2041884
display id: annotation2041884
version: 1
top level: MiddleLink
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K243005/annotation2041884/range2041884/1

https://synbiohub.org/public/igem/BBa_K243005/annotation2041884/range2041884/1
type: Range
persistent identity: range2041884
display id: range2041884
version: 1
direction: direction/forward
top level: MiddleLink
start: 1
end: 24
orientation: inline

BBa_K1680002
type: ComponentDefinition
persistent identity: BBa K1680002
display id: BBa_K1680002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Protein linker, 12aa
creator: Katharina Sporbeck
created: 2015-09-16T11:00:00Z
modified: 2015-10-27T01:28:24Z
discontinued: false
dominant: false
group u list: _2098_
m user id: 15693
owner id: 26460
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Protein coding region for the generation of 12 linker. The linker will be composed of 4 amino acids from the scars from using RFC25 standard and Serin/Glycin residues.
mutable notes: Parts are shipped in RFC25.
mutable provenance: gBlock designed by Team Tuebingen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K1680002_sequence/1

3xGS
type: ComponentDefinition
persistent identity: BBa J18921
display id: BBa_J18921
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 6aa [GS]x linker
creator: Raik Gruenberg
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Highly flexible 6 amino acid linker. Translates to gsgsgs. Codon-optimize for E. coli, yeast, mammalian.
mutable notes: * gene synthesis * codon optimized for E. coli
mutable provenance: gene synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_J18921_sequence/1

GSATLinker
type: ComponentDefinition
persistent identity: BBa K243029
display id: BBa_K243029
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GSAT Linker
creator: Freiburg Bioware09
created: 2009-10-19T11:00:00Z
modified: 2015-05-08T01:11:37Z
discontinued: false
dominant: true
experience: experience/None
group u list: _352_
m user id: 0
owner id: 4732
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is a linker, it can be used to connect two parts and add additional space between these parts. That can be necessary to avoid interactions between these parts. The GSAT linker was create to connect our protein domains Fok_a and Fok_i to an function final construct.
mutable notes: Designed for the fusion of proteins or peptides via in frame cloning, according to RFC25.
mutable provenance: Ordered by Mr.Gene.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: GSAT Linker
sequence: https://synbiohub.org/public/igem/BBa_K243029_sequence/1

GSAT Linker
type: SequenceAnnotation
persistent identity: annotation2055435
display id: annotation2055435
version: 1
top level: GSATLinker
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K243029/annotation2055435/range2055435/1

https://synbiohub.org/public/igem/BBa_K243029/annotation2055435/range2055435/1
type: Range
persistent identity: range2055435
display id: range2055435
version: 1
direction: direction/forward
top level: GSATLinker
start: 1
end: 108
orientation: inline

BBa_K416001
type: ComponentDefinition
persistent identity: BBa K416001
display id: BBa_K416001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: (Gly4Ser)3 Flexible Peptide Linker
creator: Harris Kaplan
created: 2010-05-28T11:00:00Z
modified: 2015-05-08T01:12:27Z
discontinued: false
dominant: false
group u list: _527_
m user id: 0
owner id: 6459
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a 15 amino acid flexible peptide linker protein domain that is useful for creating functional fusion proteins. The linker is to be fused in frame in between two protein domains, separating the two domains so that they each retain original functions yet will be physically connected.
mutable notes: This sequence is meant to be translated between two different protein domains, so no start or stop codon is present.
mutable provenance: The DNA came from K.D. Wittrup's lab at MIT, on the pCTCON plasmid used for yeast scFv surface display.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K416001_sequence/1

BBa_K648006
type: ComponentDefinition
persistent identity: BBa K648006
display id: BBa_K648006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Long 10AA Fusion Protein Linker with Standard 25 Prefix/Suffix
creator: Jim Rose, Alex Bina, Ben Alouidor, Brian Avison
created: 2011-07-02T11:00:00Z
modified: 2015-05-08T01:12:59Z
discontinued: false
dominant: false
experience: experience/None
group u list: _825_
m user id: 0
owner id: 9871
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the longest of the three fusion protein linkers created by the Penn State 2011 iGEM team. It encodes for the ten amino acids GENLYFQSGG and has a prefix and suffix compatible with assembly standard 25. It is used in one of the variants of our Fast-Fusion protein reporter system.
mutable notes: This part contains the AgeI and NgoMIV sites as part of it's prefix/suffix that allows it to be used in standard 25 assembly. This part was synthesized through PCR oligo synthesis methods using the following primers: Forward: TATTGAATTCGCGGCCGCTTCTAGATGGCCGGCggtgaaaatttgtattttcaa Reverse: AATACTGCAGCGGCCGCTACTAGTATTAACCGGTaccaccagattgaaaatacaaattttcacc
mutable provenance: Patrick Argos, An investigation of oligopeptides linking domains in protein tertiary structures and possible candidates for general gene fusion, Journal of Molecular Biology, Volume 211, Issue 4, 20 February 1990, Pages 943-958, ISSN 0022-2836, DOI: 10.1016/0022-2836(90)90085-Z. (http://www.sciencedirect.com/science/article/pii/002228369090085Z) Ryoichi Arai,Hiroshi Ueda,Atsushi Kitayama,Noriho Kamiya,and Teruyuki Nagamune. Design of the linkers which effectively separate domains of a bifunctional fusion protein Protein Eng. (2001) 14(8): 529-532 doi:10.1093/protein/14.8.529
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K648006_sequence/1

5xGS
type: ComponentDefinition
persistent identity: BBa J18922
display id: BBa_J18922
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 10aa [GS]x linker
creator: Raik Gruenberg
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Highly flexible 10 amino acid linker. Translates to [GS]_5. Codon-optimize for E. coli, yeast, mammalian.
mutable notes: * gene synthesis * codon optimized for E. coli * experimental distance distributions are described in Neuweiler et al. 2006 papers.
mutable provenance: gene synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_J18922_sequence/1

linker
type: ComponentDefinition
persistent identity: BBa K157013
display id: BBa_K157013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 15 aa flexible glycine-serine protein domain linker; Freiburg standard
creator: Kristian M??ller
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: A flexible, 15 aa long linker designed for fusion to/of proteins or peptides via in frame cloning. Consists of Glycine and Serine only.
mutable notes: Between BioBrick 1.0 sites this part is flanked 5' by NgoMI(=NgoMIV) and 3' AgeI(=PinAI) to facilitate in frame cloning for protein fusions.
mutable provenance: Gene synthesis by ATG:biosynthetics, optimized for expression in homo sapiens by iGEM-Team Freiburg 2008
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: GGGGSx3-Linker
sequence: https://synbiohub.org/public/igem/BBa_K157013_sequence/1

GGGGSx3-Linker
type: SequenceAnnotation
persistent identity: annotation2064780
display id: annotation2064780
version: 1
top level: linker
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157013/annotation2064780/range2064780/1

https://synbiohub.org/public/igem/BBa_K157013/annotation2064780/range2064780/1
type: Range
persistent identity: range2064780
display id: range2064780
version: 1
direction: direction/forward
top level: linker
start: 1
end: 45
orientation: inline

BBa_K1486037
type: ComponentDefinition
persistent identity: BBa K1486037
display id: BBa_K1486037
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 13 amino acids linker [GGGS GGGGS GGGS]
creator: EPFL iGem team 2014
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: 13 amino acids linker
sequence: https://synbiohub.org/public/igem/BBa_K1486037_sequence/1

13 amino acids linker
type: SequenceAnnotation
persistent identity: annotation2417686
display id: annotation2417686
version: 1
top level: BBa_K1486037
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1486037/annotation2417686/range2417686/1

https://synbiohub.org/public/igem/BBa_K1486037/annotation2417686/range2417686/1
type: Range
persistent identity: range2417686
display id: range2417686
version: 1
direction: direction/forward
top level: BBa_K1486037
start: 1
end: 39
orientation: inline

1xGS
type: ComponentDefinition
persistent identity: BBa J18920
display id: BBa_J18920
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 2aa GS linker
creator: Raik Gruenberg
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Highly flexible 2 aa linker. Translates to GS. Codon-optimal for E. coli, yeast, mammalian.
mutable notes: * gene synthesis * codon optimized for E. coli
mutable provenance: gene synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_J18920_sequence/1

BBa_K404305
type: ComponentDefinition
persistent identity: BBa K404305
display id: BBa_K404305
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Z-EGFR-1907_Long-Linker
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: Z-EGFR-1907
sequence annotation: Long-linker
sequence: https://synbiohub.org/public/igem/BBa_K404305_sequence/1

Z-EGFR-1907
type: SequenceAnnotation
persistent identity: annotation2094756
display id: annotation2094756
version: 1
top level: BBa_K404305
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404305/annotation2094756/range2094756/1

https://synbiohub.org/public/igem/BBa_K404305/annotation2094756/range2094756/1
type: Range
persistent identity: range2094756
display id: range2094756
version: 1
direction: direction/forward
top level: BBa_K404305
start: 1
end: 175
orientation: inline

Long-linker
type: SequenceAnnotation
persistent identity: annotation2094757
display id: annotation2094757
version: 1
top level: BBa_K404305
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404305/annotation2094757/range2094757/1

https://synbiohub.org/public/igem/BBa_K404305/annotation2094757/range2094757/1
type: Range
persistent identity: range2094757
display id: range2094757
version: 1
direction: direction/forward
top level: BBa_K404305
start: 181
end: 216
orientation: inline

linker
type: ComponentDefinition
persistent identity: BBa K157009
display id: BBa_K157009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Split fluorophore linker; Freiburg standard
creator: iGEM Team Freiburg 2008
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Originally, this linker was used for fusion to the N-terminus of the C-terminal half of split fluorophores: Protein interactions can be examined by BiFC[1] using complementary, non-fluorescent fragments of fluorophores[2]. Therefore, it is essential that the C-terminal fragment of the fluorophore is not restricted too much in its mobility. The linker allows orientation and adaption of the C-terminal fragment to the N-terminal fragment of the split fluorophore and, thus, the reassembly of a working fluorescent protein. It has already been used in BiFC assays and is known to serve this purpose well[3]; anyway, another, more flexible linker that could be used instead is our ???GGGGS-linker??? (Part Bba_K157010).References see "Part Design".
mutable notes: Between BioBrick 1.0 sites this part is flanked 5' by NgoMI(=NgoMIV) and 3' AgeI(=PinAI) to facilitate in frame cloning for protein fusions.
mutable provenance: Gene synthesis by ATG:biosynthetics, optimized for expression in homo sapiens by iGEM-Team Freiburg 2008
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: Split-Fluorophore Linker
sequence: https://synbiohub.org/public/igem/BBa_K157009_sequence/1

Split-Fluorophore Linker
type: SequenceAnnotation
persistent identity: annotation2040643
display id: annotation2040643
version: 1
top level: linker
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157009/annotation2040643/range2040643/1

https://synbiohub.org/public/igem/BBa_K157009/annotation2040643/range2040643/1
type: Range
persistent identity: range2040643
display id: range2040643
version: 1
direction: direction/forward
top level: linker
start: 1
end: 51
orientation: inline

BBa_K404300
type: ComponentDefinition
persistent identity: BBa K404300
display id: BBa_K404300
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SEG-Linker
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: SEG-Linker
sequence: https://synbiohub.org/public/igem/BBa_K404300_sequence/1

SEG-Linker
type: SequenceAnnotation
persistent identity: annotation2079419
display id: annotation2079419
version: 1
top level: BBa_K404300
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K404300/annotation2079419/range2079419/1

https://synbiohub.org/public/igem/BBa_K404300/annotation2079419/range2079419/1
type: Range
persistent identity: range2079419
display id: range2079419
version: 1
direction: direction/forward
top level: BBa_K404300
start: 1
end: 108
orientation: inline

BBa_K404301
type: ComponentDefinition
persistent identity: BBa K404301
display id: BBa_K404301
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GSAT-Linker
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: GSAT-Linker
sequence: https://synbiohub.org/public/igem/BBa_K404301_sequence/1

GSAT-Linker
type: SequenceAnnotation
persistent identity: annotation2079420
display id: annotation2079420
version: 1
top level: BBa_K404301
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K404301/annotation2079420/range2079420/1

https://synbiohub.org/public/igem/BBa_K404301/annotation2079420/range2079420/1
type: Range
persistent identity: range2079420
display id: range2079420
version: 1
direction: direction/forward
top level: BBa_K404301
start: 1
end: 108
orientation: inline

BBa_K1777019
type: ComponentDefinition
persistent identity: BBa K1777019
display id: BBa_K1777019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: flexible linker
creator: Xuhang Li
created: 2015-09-17T11:00:00Z
modified: 2015-09-20T02:06:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2199_
m user id: 27798
owner id: 25645
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This a flexible linker combined by two former linkers, which can be easily connect to the end of protein with overlap pcr.
mutable notes: when we chose a linker for our fusion protein，we would have wanted to use -gggsgggs- linker. But we found that such a tandem repeating linker is hard to work well with overlap PCR because the OE region should be unique. Therefore, we improved the original part by change the first GGGS to a carefully selected natural linker from linker database to avoid repeating sequence. Besides, we used the selected natural linker to adjust its length to about 50A.
mutable provenance: BBa_K1486003
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: natural linker
sequence annotation: GS linker
sequence: https://synbiohub.org/public/igem/BBa_K1777019_sequence/1

natural linker
type: SequenceAnnotation
persistent identity: annotation2476148
display id: annotation2476148
version: 1
top level: BBa_K1777019
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1777019/annotation2476148/range2476148/1

https://synbiohub.org/public/igem/BBa_K1777019/annotation2476148/range2476148/1
type: Range
persistent identity: range2476148
display id: range2476148
version: 1
direction: direction/forward
top level: BBa_K1777019
start: 1
end: 21
orientation: inline

GS linker
type: SequenceAnnotation
persistent identity: annotation2476149
display id: annotation2476149
version: 1
top level: BBa_K1777019
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1777019/annotation2476149/range2476149/1

https://synbiohub.org/public/igem/BBa_K1777019/annotation2476149/range2476149/1
type: Range
persistent identity: range2476149
display id: range2476149
version: 1
direction: direction/forward
top level: BBa_K1777019
start: 22
end: 36
orientation: inline

BBa_K404303
type: ComponentDefinition
persistent identity: BBa K404303
display id: BBa_K404303
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Z-EGFR-1907_Short-Linker
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: Short-linker
sequence annotation: Z-EGFR-1907
sequence: https://synbiohub.org/public/igem/BBa_K404303_sequence/1

Short-linker
type: SequenceAnnotation
persistent identity: annotation2094743
display id: annotation2094743
version: 1
top level: BBa_K404303
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404303/annotation2094743/range2094743/1

https://synbiohub.org/public/igem/BBa_K404303/annotation2094743/range2094743/1
type: Range
persistent identity: range2094743
display id: range2094743
version: 1
direction: direction/forward
top level: BBa_K404303
start: 181
end: 192
orientation: inline

Z-EGFR-1907
type: SequenceAnnotation
persistent identity: annotation2094742
display id: annotation2094742
version: 1
top level: BBa_K404303
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404303/annotation2094742/range2094742/1

https://synbiohub.org/public/igem/BBa_K404303/annotation2094742/range2094742/1
type: Range
persistent identity: range2094742
display id: range2094742
version: 1
direction: direction/forward
top level: BBa_K404303
start: 1
end: 175
orientation: inline

LongLinker
type: ComponentDefinition
persistent identity: BBa K243006
display id: BBa_K243006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Long Linker (Gly-Gly-Ser-Gly)x3
creator: Freiburg Bioware09
created: 2009-10-13T11:00:00Z
modified: 2015-05-08T01:11:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _352_
m user id: 0
owner id: 4732
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is a linker, it can be used to connect two parts and add additional space between these parts. That can be necessary to avoid interactions between these parts. We used this part to connect our protein domains with our lipocalin domains, for our project the universal endonuclease.The sequence produced the aminoacids Gly-Gly-Ser-Gly-Gly-Gly-Ser-Gly-Gly-Gly-Ser-Gly.
mutable notes: None.
mutable provenance: Oligos synthesized by sigma. Hybridized by PCR.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence annotation: Long Linker
sequence: https://synbiohub.org/public/igem/BBa_K243006_sequence/1

Long Linker
type: SequenceAnnotation
persistent identity: annotation2041937
display id: annotation2041937
version: 1
top level: LongLinker
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K243006/annotation2041937/range2041937/1

https://synbiohub.org/public/igem/BBa_K243006/annotation2041937/range2041937/1
type: Range
persistent identity: range2041937
display id: range2041937
version: 1
direction: direction/forward
top level: LongLinker
start: 1
end: 36
orientation: inline

ShortLinke
type: ComponentDefinition
persistent identity: BBa K243004
display id: BBa_K243004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Short Linker (Gly-Gly-Ser-Gly)
creator: Freiburg Bioware09
created: 2009-10-11T11:00:00Z
modified: 2015-05-08T01:11:37Z
discontinued: false
dominant: true
experience: experience/None
group u list: _352_
m user id: 0
owner id: 4732
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This linker was used to connect two different parts. The sequence produced the aminoacids Gly-Gly-Ser-Gly.
mutable notes: None.
mutable provenance: Oligos synthesized by sigma. Hybridized by PCR.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence annotation: Short Linker
sequence: https://synbiohub.org/public/igem/BBa_K243004_sequence/1

Short Linker
type: SequenceAnnotation
persistent identity: annotation2041881
display id: annotation2041881
version: 1
top level: ShortLinke
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K243004/annotation2041881/range2041881/1

https://synbiohub.org/public/igem/BBa_K243004/annotation2041881/range2041881/1
type: Range
persistent identity: range2041881
display id: range2041881
version: 1
direction: direction/forward
top level: ShortLinke
start: 1
end: 12
orientation: inline

BBa_K648005
type: ComponentDefinition
persistent identity: BBa K648005
display id: BBa_K648005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Short Fusion Protein Linker: GGSG with standard 25 prefix/suffix
creator: Jim Rose, Alex Bina, Ben Alouidor, Brian Avison
created: 2011-07-02T11:00:00Z
modified: 2015-05-08T01:12:59Z
discontinued: false
dominant: false
experience: experience/None
group u list: _825_
m user id: 0
owner id: 9871
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the shortest of the three fusion protein linkers created by the Penn State 2011 iGEM team. It encodes for the four amino acids GGSG and has a prefix and suffix compatible with assembly standard 25. It is used in one of the variants of our Fast-Fusion protein reporter system.
mutable notes: This part contains the AgeI and NgoMIV sites as part of it's prefix/suffix that allows it to be used in standard 25 assembly. This part was synthesized through PCR oligo synthesis methods using the following primers: Forward (57.78 degrees) TATTGAATTCGCGGCCGCTTCTAGATGGCCGGCggtggttctggtACC Reverse (57.78 degrees) AATACTGCAGCGGCCGCTACTAGTATTAACCGGTaccagaaccaccG
mutable provenance: Patrick Argos, An investigation of oligopeptides linking domains in protein tertiary structures and possible candidates for general gene fusion, Journal of Molecular Biology, Volume 211, Issue 4, 20 February 1990, Pages 943-958, ISSN 0022-2836, DOI: 10.1016/0022-2836(90)90085-Z. (http://www.sciencedirect.com/science/article/pii/002228369090085Z) Ryoichi Arai,Hiroshi Ueda,Atsushi Kitayama,Noriho Kamiya,and Teruyuki Nagamune. Design of the linkers which effectively separate domains of a bifunctional fusion protein Protein Eng. (2001) 14(8): 529-532 doi:10.1093/protein/14.8.529
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K648005_sequence/1

BBa_K648007
type: ComponentDefinition
persistent identity: BBa K648007
display id: BBa_K648007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Medium 6AA Fusion Protein Linker: GGSGGS with Standard 25 Prefix/Suffix
creator: Jim Rose
created: 2011-07-02T11:00:00Z
modified: 2015-05-08T01:12:59Z
discontinued: false
dominant: false
experience: experience/None
group u list: _825_
m user id: 0
owner id: 9871
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the mid-sized linker of the three fusion protein linkers created by the Penn State 2011 iGEM team. It encodes for the six amino acids GGSGGS and has a prefix and suffix compatible with assembly standard 25. It is used in one of the variants of our Fast-Fusion protein reporter system.
mutable notes: This part contains the AgeI and NgoMIV sites as part of it's prefix/suffix that allows it to be used in standard 25 assembly. This part was synthesized through PCR oligo synthesis methods using the following primers: Forward: 5??? TATTGAATTCGCGGCCGCTTCTAGATGGCCGGCggaggttcagg 3??? Reverse: 5???AATACTGCAGCGGCCGCTACTAGTATTAACCGGTgctgcctcctgaacctccGC 3'
mutable provenance: The inspiration for this part came from the linker used in the 2010 Imperial College London iGEM team GFP-Xyle fusion part: BBa_K316007 Other Sources: Patrick Argos, An investigation of oligopeptides linking domains in protein tertiary structures and possible candidates for general gene fusion, Journal of Molecular Biology, Volume 211, Issue 4, 20 February 1990, Pages 943-958, ISSN 0022-2836, DOI: 10.1016/0022-2836(90)90085-Z. (http://www.sciencedirect.com/science/article/pii/002228369090085Z) Ryoichi Arai,Hiroshi Ueda,Atsushi Kitayama,Noriho Kamiya,and Teruyuki Nagamune. Design of the linkers which effectively separate domains of a bifunctional fusion protein Protein Eng. (2001) 14(8): 529-532 doi:10.1093/protein/14.8.529
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K648007_sequence/1

BBa_K105012
type: ComponentDefinition
persistent identity: BBa K105012
display id: BBa_K105012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 10 aa flexible protein domain linker
creator: Manuel Gersbacher, Katja Karstens
created: 2008-10-18T11:00:00Z
modified: 2015-05-08T01:08:52Z
discontinued: false
dominant: true
experience: experience/None
group u list: _253_
m user id: 0
owner id: 3313
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is a 10 amino acides long linker peptide which can be used to join protein domains together in a flexible way. So fusion proteins with variable DNA-binding and activation or repression domains might be assembled. <br\n>
mutable notes: To enable the fusion with other domains in frame the vector of this BioBrick has no base pair in between the restriction side and the BioBrick. Furthermore, this coding sequence does not include a start codon.<br\n> For more information about this issus, see:<br\n> Phillips, I.E. and Silver, P.A. "A new Biobrick Assembly Strategy Designed for Facile Protein Engineering." <br\n> DSpace http://hdl.handle.net/1721.1/32535 (2006).
mutable provenance: Oligos with a yeast optimized coding for the peptide GENLYFQSGG have been ordered <br\n> The amino acide sequence corresponds to the interdomaine linker of xxx. ''paper''
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence: https://synbiohub.org/public/igem/BBa_K105012_sequence/1

PLrigid
type: ComponentDefinition
persistent identity: BBa J176131
display id: BBa_J176131
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PLrigid
creator: Karmella Haynes
created: 2011-10-27T11:00:00Z
modified: 2015-08-31T04:08:35Z
discontinued: false
dominant: false
experience: experience/None
group u list: _863_
m user id: 0
owner id: 10865
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: (EAAAR)4 peptide linker, rigid; alpha helix secondary structure; see Yan et al. 2007
mutable notes: TBA
mutable provenance: TBA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: EAAAR
sequence: https://synbiohub.org/public/igem/BBa_J176131_sequence/1

EAAAR
type: SequenceAnnotation
persistent identity: annotation2179297
display id: annotation2179297
version: 1
top level: PLrigid
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_J176131/annotation2179297/range2179297/1

https://synbiohub.org/public/igem/BBa_J176131/annotation2179297/range2179297/1
type: Range
persistent identity: range2179297
display id: range2179297
version: 1
direction: direction/forward
top level: PLrigid
start: 16
end: 30
orientation: inline

EAAAR
type: SequenceAnnotation
persistent identity: annotation2179296
display id: annotation2179296
version: 1
top level: PLrigid
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_J176131/annotation2179296/range2179296/1

https://synbiohub.org/public/igem/BBa_J176131/annotation2179296/range2179296/1
type: Range
persistent identity: range2179296
display id: range2179296
version: 1
direction: direction/forward
top level: PLrigid
start: 1
end: 15
orientation: inline

EAAAR
type: SequenceAnnotation
persistent identity: annotation2179299
display id: annotation2179299
version: 1
top level: PLrigid
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_J176131/annotation2179299/range2179299/1

https://synbiohub.org/public/igem/BBa_J176131/annotation2179299/range2179299/1
type: Range
persistent identity: range2179299
display id: range2179299
version: 1
direction: direction/forward
top level: PLrigid
start: 46
end: 60
orientation: inline

EAAAR
type: SequenceAnnotation
persistent identity: annotation2179298
display id: annotation2179298
version: 1
top level: PLrigid
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_J176131/annotation2179298/range2179298/1

https://synbiohub.org/public/igem/BBa_J176131/annotation2179298/range2179298/1
type: Range
persistent identity: range2179298
display id: range2179298
version: 1
direction: direction/forward
top level: PLrigid
start: 31
end: 45
orientation: inline

BBa_K404304
type: ComponentDefinition
persistent identity: BBa K404304
display id: BBa_K404304
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Z-EGFR-1907_Middle-Linker
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: Middle-linker
sequence annotation: Z-EGFR-1907
sequence: https://synbiohub.org/public/igem/BBa_K404304_sequence/1

Middle-linker
type: SequenceAnnotation
persistent identity: annotation2094746
display id: annotation2094746
version: 1
top level: BBa_K404304
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404304/annotation2094746/range2094746/1

https://synbiohub.org/public/igem/BBa_K404304/annotation2094746/range2094746/1
type: Range
persistent identity: range2094746
display id: range2094746
version: 1
direction: direction/forward
top level: BBa_K404304
start: 181
end: 204
orientation: inline

Z-EGFR-1907
type: SequenceAnnotation
persistent identity: annotation2094745
display id: annotation2094745
version: 1
top level: BBa_K404304
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404304/annotation2094745/range2094745/1

https://synbiohub.org/public/igem/BBa_K404304/annotation2094745/range2094745/1
type: Range
persistent identity: range2094745
display id: range2094745
version: 1
direction: direction/forward
top level: BBa_K404304
start: 1
end: 175
orientation: inline

linker
type: ComponentDefinition
persistent identity: BBa K133132
display id: BBa_K133132
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 8 aa protein domain linker
creator: Jan Lonzaric
created: 2008-10-23T11:00:00Z
modified: 2015-05-08T01:09:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _231_
m user id: 0
owner id: 3358
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence: https://synbiohub.org/public/igem/BBa_K133132_sequence/1

BBa_K1486003
type: ComponentDefinition
persistent identity: BBa K1486003
display id: BBa_K1486003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Flexible linker 2x (GGGS)
creator: iGem EPFL 2014
created: 2014-08-11T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Flexible linker used to fuse two proteins.
mutable notes: Designed considering the flexibility and separation needed between our fused proteins.
mutable provenance: Synthetic.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K1486003_sequence/1