//proteindomain/binding
type: Collection
persistent identity: proteindomain binding
display id: proteindomain_binding
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: GLucFrag2
member: ZipR34
member: BBa_K1478001
member: BBa_K801038
member: occupyDNAP
member: BBa_K772002
member: BBa_K801042
member: CBDcex
member: BBa_K1333200
member: BBa_J18940
member: flyPCD
member: BBa_J18933
member: BBa_J18938
member: BBa_K1404006
member: SP-AntiNIP
member: BBa_K404304
member: BBa_J18947
member: BBa_K801040
member: FRB
member: BBa_J18952
member: BBa_J18956
member: BBa_K801031
member: BBa_J18951
member: BBa_K1333203
member: BBa_K1979001
member: BBa_K404306
member: CBDclos(T7
member: BBa_K1404008
member: BBa_K157004
member: BBa_J18944
member: Lov2
member: BBa_J18957
member: BBa_J18950
member: BBa_K801043
member: N4 gp8
member: BBa_K863113
member: BBa_K1404007
member: BBa_J18945
member: BBa_K772100
member: BBa_K1478002
member: GLucFrag1
member: BBa_K404303
member: BBa_J18937
member: BBa_K1994008
member: CBDclos(J6
member: BBa_K801039
member: BBa_J18953
member: FKBP12
member: BBa_J18955
member: CBDcex(T7)
member: BBa_J18934
member: BBa_K801041
member: BBa_K157003
member: BBa_J18939
member: BBa_K404305
member: BBa_J18943
member: BBa_J18948
member: BBa_K352001
member: CBDclos
member: BBa_J18935
member: BBa_K1333202
member: BBa_K404314
member: CBDcex(J61
member: BBa_K801035
member: BBa_J18942
member: BBa_J18936

BBa_K1333201
type: ComponentDefinition
persistent identity: BBa K1333201
display id: BBa_K1333201
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lambda cI-LGF2(D78A)
creator: Yanwen Xie
created: 2014-09-08T11:00:00Z
modified: 2015-05-08T01:09:58Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1708_
m user id: 0
owner id: 15838
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Fuse lambda cI with LGF2 mutated from Asp to Ala at site 78. As one of the components in Bacterial Two-hybrid System, it causes a less interaction with GAL11P compared to the nomalized WT.
mutable notes: Mutation of GAC to GCC LGF2 at site 78.
mutable provenance: From Agilent Technologies' BacterioMatch ⅡTwo-Hybrid System Vector Kit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: TGA stop codon
sequence annotation: lambda cI
sequence annotation: ATG start codon
sequence annotation: LGF2(D78A)
sequence: https://synbiohub.org/public/igem/BBa_K1333201_sequence/1

TGA stop codon
type: SequenceAnnotation
persistent identity: annotation2382834
display id: annotation2382834
version: 1
top level: BBa_K1333201
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1333201/annotation2382834/range2382834/1

https://synbiohub.org/public/igem/BBa_K1333201/annotation2382834/range2382834/1
type: Range
persistent identity: range2382834
display id: range2382834
version: 1
direction: direction/forward
top level: BBa_K1333201
start: 841
end: 843
orientation: inline

lambda cI
type: SequenceAnnotation
persistent identity: annotation2382831
display id: annotation2382831
version: 1
top level: BBa_K1333201
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1333201/annotation2382831/range2382831/1

https://synbiohub.org/public/igem/BBa_K1333201/annotation2382831/range2382831/1
type: Range
persistent identity: range2382831
display id: range2382831
version: 1
direction: direction/forward
top level: BBa_K1333201
start: 1
end: 711
orientation: inline

ATG start codon
type: SequenceAnnotation
persistent identity: annotation2382833
display id: annotation2382833
version: 1
top level: BBa_K1333201
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1333201/annotation2382833/range2382833/1

https://synbiohub.org/public/igem/BBa_K1333201/annotation2382833/range2382833/1
type: Range
persistent identity: range2382833
display id: range2382833
version: 1
direction: direction/forward
top level: BBa_K1333201
start: 1
end: 3
orientation: inline

LGF2(D78A)
type: SequenceAnnotation
persistent identity: annotation2382832
display id: annotation2382832
version: 1
top level: BBa_K1333201
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1333201/annotation2382832/range2382832/1

https://synbiohub.org/public/igem/BBa_K1333201/annotation2382832/range2382832/1
type: Range
persistent identity: range2382832
display id: range2382832
version: 1
direction: direction/forward
top level: BBa_K1333201
start: 721
end: 840
orientation: inline

BBa_J18936
type: ComponentDefinition
persistent identity: BBa J18936
display id: BBa_J18936
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FKBP ~ mCherry
creator: Raik Gruenberg
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
discontinued: false
dominant: false
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: P04 expression cassette from Gruenberg et al. 2010 series of interaction reporter proteins. All proteins share the same architecture: * T7Start-domain1-5xGS-domain2-preSCsite-HisTag-T7Stop Only Domains1 and 2 vary and are taken from a list of protein interaction input and output (readout) parts. ===Expression=== Proteins were expressed in E. coli BL21 using T7 Polymerase and were purified by His-tag affinity. ===Reference=== * Gruenberg, Ferrar, van der Sloot, Serrano (2010): Building Blocks for Protein Interaction Devices
mutable notes: Constructs were assembled according to R.F.C. 25. All parts were codon optimized for E. coli.
mutable provenance: synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0105
component: BBa_J18932
component: BBa_J18922
component: BBa_J18912
component: BBa_J18909
component: BBa_J18913
component: BBa_J18919
component: BBa_J18925
sequence annotation: BBa_B0105
sequence annotation: BBa_J18919
sequence annotation: BBa_J18922
sequence annotation: BBa_J18913
sequence annotation: BBa_J18912
sequence annotation: BBa_J18909
sequence annotation: BBa_J18932
sequence annotation: BBa_J18925
sequence: https://synbiohub.org/public/igem/BBa_J18936_sequence/1

BBa_B0105
type: Component
persistent identity: component2066395
display id: component2066395
version: 1
top level: BBa_J18936
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_J18932
type: Component
persistent identity: component2066386
display id: component2066386
version: 1
top level: BBa_J18936
access: public
definition: https://synbiohub.org/public/igem/BBa_J18932/1

BBa_J18922
type: Component
persistent identity: component2066383
display id: component2066383
version: 1
top level: BBa_J18936
access: public
definition: https://synbiohub.org/public/igem/BBa_J18922/1

BBa_J18912
type: Component
persistent identity: component2066377
display id: component2066377
version: 1
top level: BBa_J18936
access: public
definition: https://synbiohub.org/public/igem/BBa_J18912/1

BBa_J18909
type: Component
persistent identity: component2066393
display id: component2066393
version: 1
top level: BBa_J18936
access: public
definition: https://synbiohub.org/public/igem/BBa_J18909/1

BBa_J18913
type: Component
persistent identity: component2066397
display id: component2066397
version: 1
top level: BBa_J18936
access: public
definition: https://synbiohub.org/public/igem/BBa_J18913/1

BBa_B0105
type: Component
persistent identity: component2066382
display id: component2066382
version: 1
top level: BBa_J18936
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_B0105
type: Component
persistent identity: component2066391
display id: component2066391
version: 1
top level: BBa_J18936
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_J18919
type: Component
persistent identity: component2066389
display id: component2066389
version: 1
top level: BBa_J18936
access: public
definition: https://synbiohub.org/public/igem/BBa_J18919/1

BBa_B0105
type: Component
persistent identity: component2066379
display id: component2066379
version: 1
top level: BBa_J18936
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_B0105
type: Component
persistent identity: component2066388
display id: component2066388
version: 1
top level: BBa_J18936
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_J18925
type: Component
persistent identity: component2066380
display id: component2066380
version: 1
top level: BBa_J18936
access: public
definition: FKBP12

BBa_B0105
type: Component
persistent identity: component2066385
display id: component2066385
version: 1
top level: BBa_J18936
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066395
display id: annotation2066395
version: 1
top level: BBa_J18936
location: https://synbiohub.org/public/igem/BBa_J18936/annotation2066395/range2066395/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18936/annotation2066395/range2066395/1
type: Range
persistent identity: range2066395
display id: range2066395
version: 1
direction: direction/reverse
top level: BBa_J18936
start: 1212
end: 1217
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066382
display id: annotation2066382
version: 1
top level: BBa_J18936
location: https://synbiohub.org/public/igem/BBa_J18936/annotation2066382/range2066382/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18936/annotation2066382/range2066382/1
type: Range
persistent identity: range2066382
display id: range2066382
version: 1
direction: direction/reverse
top level: BBa_J18936
start: 411
end: 416
orientation: inline

BBa_J18919
type: SequenceAnnotation
persistent identity: annotation2066389
display id: annotation2066389
version: 1
top level: BBa_J18936
location: https://synbiohub.org/public/igem/BBa_J18936/annotation2066389/range2066389/1
component: BBa_J18919

https://synbiohub.org/public/igem/BBa_J18936/annotation2066389/range2066389/1
type: Range
persistent identity: range2066389
display id: range2066389
version: 1
direction: direction/reverse
top level: BBa_J18936
start: 1164
end: 1187
orientation: inline

BBa_J18922
type: SequenceAnnotation
persistent identity: annotation2066383
display id: annotation2066383
version: 1
top level: BBa_J18936
location: https://synbiohub.org/public/igem/BBa_J18936/annotation2066383/range2066383/1
component: BBa_J18922

https://synbiohub.org/public/igem/BBa_J18936/annotation2066383/range2066383/1
type: Range
persistent identity: range2066383
display id: range2066383
version: 1
direction: direction/reverse
top level: BBa_J18936
start: 417
end: 446
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066388
display id: annotation2066388
version: 1
top level: BBa_J18936
location: https://synbiohub.org/public/igem/BBa_J18936/annotation2066388/range2066388/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18936/annotation2066388/range2066388/1
type: Range
persistent identity: range2066388
display id: range2066388
version: 1
direction: direction/reverse
top level: BBa_J18936
start: 1158
end: 1163
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066391
display id: annotation2066391
version: 1
top level: BBa_J18936
location: https://synbiohub.org/public/igem/BBa_J18936/annotation2066391/range2066391/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18936/annotation2066391/range2066391/1
type: Range
persistent identity: range2066391
display id: range2066391
version: 1
direction: direction/reverse
top level: BBa_J18936
start: 1188
end: 1193
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066379
display id: annotation2066379
version: 1
top level: BBa_J18936
location: https://synbiohub.org/public/igem/BBa_J18936/annotation2066379/range2066379/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18936/annotation2066379/range2066379/1
type: Range
persistent identity: range2066379
display id: range2066379
version: 1
direction: direction/reverse
top level: BBa_J18936
start: 84
end: 89
orientation: inline

BBa_J18913
type: SequenceAnnotation
persistent identity: annotation2066397
display id: annotation2066397
version: 1
top level: BBa_J18936
location: https://synbiohub.org/public/igem/BBa_J18936/annotation2066397/range2066397/1
component: BBa_J18913

https://synbiohub.org/public/igem/BBa_J18936/annotation2066397/range2066397/1
type: Range
persistent identity: range2066397
display id: range2066397
version: 1
direction: direction/reverse
top level: BBa_J18936
start: 1218
end: 1352
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066385
display id: annotation2066385
version: 1
top level: BBa_J18936
location: https://synbiohub.org/public/igem/BBa_J18936/annotation2066385/range2066385/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18936/annotation2066385/range2066385/1
type: Range
persistent identity: range2066385
display id: range2066385
version: 1
direction: direction/reverse
top level: BBa_J18936
start: 447
end: 452
orientation: inline

BBa_J18912
type: SequenceAnnotation
persistent identity: annotation2066377
display id: annotation2066377
version: 1
top level: BBa_J18936
location: https://synbiohub.org/public/igem/BBa_J18936/annotation2066377/range2066377/1
component: BBa_J18912

https://synbiohub.org/public/igem/BBa_J18936/annotation2066377/range2066377/1
type: Range
persistent identity: range2066377
display id: range2066377
version: 1
direction: direction/reverse
top level: BBa_J18936
start: 1
end: 83
orientation: inline

BBa_J18909
type: SequenceAnnotation
persistent identity: annotation2066393
display id: annotation2066393
version: 1
top level: BBa_J18936
location: https://synbiohub.org/public/igem/BBa_J18936/annotation2066393/range2066393/1
component: BBa_J18909

https://synbiohub.org/public/igem/BBa_J18936/annotation2066393/range2066393/1
type: Range
persistent identity: range2066393
display id: range2066393
version: 1
direction: direction/reverse
top level: BBa_J18936
start: 1194
end: 1211
orientation: inline

BBa_J18932
type: SequenceAnnotation
persistent identity: annotation2066386
display id: annotation2066386
version: 1
top level: BBa_J18936
location: https://synbiohub.org/public/igem/BBa_J18936/annotation2066386/range2066386/1
component: BBa_J18932

https://synbiohub.org/public/igem/BBa_J18936/annotation2066386/range2066386/1
type: Range
persistent identity: range2066386
display id: range2066386
version: 1
direction: direction/reverse
top level: BBa_J18936
start: 453
end: 1157
orientation: inline

BBa_J18925
type: SequenceAnnotation
persistent identity: annotation2066380
display id: annotation2066380
version: 1
top level: BBa_J18936
location: https://synbiohub.org/public/igem/BBa_J18936/annotation2066380/range2066380/1
component: BBa_J18925

https://synbiohub.org/public/igem/BBa_J18936/annotation2066380/range2066380/1
type: Range
persistent identity: range2066380
display id: range2066380
version: 1
direction: direction/reverse
top level: BBa_J18936
start: 90
end: 410
orientation: inline

BBa_K1979001
type: ComponentDefinition
persistent identity: BBa K1979001
display id: BBa_K1979001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CBD (cbh2)
creator: MA Xinyi
created: 2016-08-19T11:00:00Z
modified: 2016-08-24T10:46:39Z
discontinued: false
dominant: false
group u list: _2446_
m user id: 29797
owner id: 29797
owning group id: 9
status: status/Planning
bookmark: false
mutable description: The sequence codes for cellulose binding domain cbh2 in prokaryotic cells.
mutable notes: Restriction sites Xho I and Bamh I are added.
mutable provenance: The sequence is retrieved from Genbank.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: CBD cbh2
sequence: https://synbiohub.org/public/igem/BBa_K1979001_sequence/1

CBD cbh2
type: SequenceAnnotation
persistent identity: annotation2481557
display id: annotation2481557
version: 1
top level: BBa_K1979001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1979001/annotation2481557/range2481557/1

https://synbiohub.org/public/igem/BBa_K1979001/annotation2481557/range2481557/1
type: Range
persistent identity: range2481557
display id: range2481557
version: 1
direction: direction/forward
top level: BBa_K1979001
start: 1
end: 147
orientation: inline

BBa_K801038
type: ComponentDefinition
persistent identity: BBa K801038
display id: BBa_K801038
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PhyB-linker-Gal4bd
creator: Dong-Jiunn Jeffery TRUONG
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
group u list: _1057_
m user id: 0
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Composite part of BBa_K801031, Bba_K365005 and BBa_K801032
mutable notes: -
mutable provenance: Composite part of BBa_K801031, Bba_K365005 and BBa_K801032
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: Essential Cys for chromophore attachement of phycocyanobilin (PCB)
sequence annotation: BBa_K801032
sequence annotation: BBa_K801037
sequence annotation: Essential Arg for photoconvertible binding of PIF3
sequence annotation: Essential Gly for photoconvertible binding of PIF3
sequence annotation: BBa_K365005
sequence annotation: BBa_K801031
sequence annotation: RFC25 fusion scar
sequence: https://synbiohub.org/public/igem/BBa_K801038_sequence/1

Essential Cys for chromophore attachement of phycocyanobilin (PCB)
type: SequenceAnnotation
persistent identity: annotation2198916
display id: annotation2198916
version: 1
top level: BBa_K801038
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K801038/annotation2198916/range2198916/1

https://synbiohub.org/public/igem/BBa_K801038/annotation2198916/range2198916/1
type: Range
persistent identity: range2198916
display id: range2198916
version: 1
direction: direction/forward
top level: BBa_K801038
start: 1066
end: 1068
orientation: inline

BBa_K801032
type: SequenceAnnotation
persistent identity: annotation2198920
display id: annotation2198920
version: 1
top level: BBa_K801038
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801038/annotation2198920/range2198920/1

https://synbiohub.org/public/igem/BBa_K801038/annotation2198920/range2198920/1
type: Range
persistent identity: range2198920
display id: range2198920
version: 1
direction: direction/forward
top level: BBa_K801038
start: 2794
end: 3231
orientation: inline

BBa_K801037
type: SequenceAnnotation
persistent identity: annotation2198921
display id: annotation2198921
version: 1
top level: BBa_K801038
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801038/annotation2198921/range2198921/1

https://synbiohub.org/public/igem/BBa_K801038/annotation2198921/range2198921/1
type: Range
persistent identity: range2198921
display id: range2198921
version: 1
direction: direction/forward
top level: BBa_K801038
start: 2728
end: 3231
orientation: inline

Essential Arg for photoconvertible binding of PIF3
type: SequenceAnnotation
persistent identity: annotation2198912
display id: annotation2198912
version: 1
top level: BBa_K801038
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K801038/annotation2198912/range2198912/1

https://synbiohub.org/public/igem/BBa_K801038/annotation2198912/range2198912/1
type: Range
persistent identity: range2198912
display id: range2198912
version: 1
direction: direction/forward
top level: BBa_K801038
start: 325
end: 327
orientation: inline

Essential Gly for photoconvertible binding of PIF3
type: SequenceAnnotation
persistent identity: annotation2198914
display id: annotation2198914
version: 1
top level: BBa_K801038
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K801038/annotation2198914/range2198914/1

https://synbiohub.org/public/igem/BBa_K801038/annotation2198914/range2198914/1
type: Range
persistent identity: range2198914
display id: range2198914
version: 1
direction: direction/forward
top level: BBa_K801038
start: 1039
end: 1041
orientation: inline

BBa_K365005
type: SequenceAnnotation
persistent identity: annotation2198918
display id: annotation2198918
version: 1
top level: BBa_K801038
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801038/annotation2198918/range2198918/1

https://synbiohub.org/public/igem/BBa_K801038/annotation2198918/range2198918/1
type: Range
persistent identity: range2198918
display id: range2198918
version: 1
direction: direction/forward
top level: BBa_K801038
start: 2728
end: 2787
orientation: inline

BBa_K801031
type: SequenceAnnotation
persistent identity: annotation2198917
display id: annotation2198917
version: 1
top level: BBa_K801038
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801038/annotation2198917/range2198917/1

https://synbiohub.org/public/igem/BBa_K801038/annotation2198917/range2198917/1
type: Range
persistent identity: range2198917
display id: range2198917
version: 1
direction: direction/forward
top level: BBa_K801038
start: 1
end: 2721
orientation: inline

RFC25 fusion scar
type: SequenceAnnotation
persistent identity: annotation2198923
display id: annotation2198923
version: 1
top level: BBa_K801038
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801038/annotation2198923/range2198923/1

https://synbiohub.org/public/igem/BBa_K801038/annotation2198923/range2198923/1
type: Range
persistent identity: range2198923
display id: range2198923
version: 1
direction: direction/forward
top level: BBa_K801038
start: 2722
end: 2727
orientation: inline

RFC25 fusion scar
type: SequenceAnnotation
persistent identity: annotation2198919
display id: annotation2198919
version: 1
top level: BBa_K801038
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801038/annotation2198919/range2198919/1

https://synbiohub.org/public/igem/BBa_K801038/annotation2198919/range2198919/1
type: Range
persistent identity: range2198919
display id: range2198919
version: 1
direction: direction/forward
top level: BBa_K801038
start: 2788
end: 2793
orientation: inline

Essential Gly for photoconvertible binding of PIF3
type: SequenceAnnotation
persistent identity: annotation2198913
display id: annotation2198913
version: 1
top level: BBa_K801038
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K801038/annotation2198913/range2198913/1

https://synbiohub.org/public/igem/BBa_K801038/annotation2198913/range2198913/1
type: Range
persistent identity: range2198913
display id: range2198913
version: 1
direction: direction/forward
top level: BBa_K801038
start: 328
end: 330
orientation: inline

Essential Arg for photoconvertible binding of PIF3
type: SequenceAnnotation
persistent identity: annotation2198915
display id: annotation2198915
version: 1
top level: BBa_K801038
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K801038/annotation2198915/range2198915/1

https://synbiohub.org/public/igem/BBa_K801038/annotation2198915/range2198915/1
type: Range
persistent identity: range2198915
display id: range2198915
version: 1
direction: direction/forward
top level: BBa_K801038
start: 1051
end: 1053
orientation: inline

BBa_K1909004
type: ComponentDefinition
persistent identity: BBa K1909004
display id: BBa_K1909004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NOD1 LRR Domain
creator: Daniel Wedemeyer
created: 2016-10-13T11:00:00Z
modified: 2016-10-14T11:21:00Z
discontinued: false
dominant: false
group u list: _2375_
m user id: 29525
owner id: 29525
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Coming soon
mutable notes: coming soon
mutable provenance: NOD1 (ncbi: https://www.ncbi.nlm.nih.gov/gene/10392)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Changed CTG to TTG
sequence annotation: Changed CTA to TTA
sequence annotation: NOD1
sequence annotation: Changed GAC to GAT
sequence annotation: Changed CTG to CTA
sequence: https://synbiohub.org/public/igem/BBa_K1909004_sequence/1

Changed CTG to TTG
type: SequenceAnnotation
persistent identity: annotation2507292
display id: annotation2507292
version: 1
top level: BBa_K1909004
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K1909004/annotation2507292/range2507292/1

https://synbiohub.org/public/igem/BBa_K1909004/annotation2507292/range2507292/1
type: Range
persistent identity: range2507292
display id: range2507292
version: 1
direction: direction/forward
top level: BBa_K1909004
start: 700
end: 702
orientation: inline

Changed CTA to TTA
type: SequenceAnnotation
persistent identity: annotation2507293
display id: annotation2507293
version: 1
top level: BBa_K1909004
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K1909004/annotation2507293/range2507293/1

https://synbiohub.org/public/igem/BBa_K1909004/annotation2507293/range2507293/1
type: Range
persistent identity: range2507293
display id: range2507293
version: 1
direction: direction/forward
top level: BBa_K1909004
start: 718
end: 720
orientation: inline

NOD1
type: SequenceAnnotation
persistent identity: annotation2507199
display id: annotation2507199
version: 1
top level: BBa_K1909004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1909004/annotation2507199/range2507199/1

https://synbiohub.org/public/igem/BBa_K1909004/annotation2507199/range2507199/1
type: Range
persistent identity: range2507199
display id: range2507199
version: 1
direction: direction/forward
top level: BBa_K1909004
start: 1
end: 969
orientation: inline

Changed GAC to GAT
type: SequenceAnnotation
persistent identity: annotation2507200
display id: annotation2507200
version: 1
top level: BBa_K1909004
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K1909004/annotation2507200/range2507200/1

https://synbiohub.org/public/igem/BBa_K1909004/annotation2507200/range2507200/1
type: Range
persistent identity: range2507200
display id: range2507200
version: 1
direction: direction/forward
top level: BBa_K1909004
start: 181
end: 183
orientation: inline

Changed CTG to TTG
type: SequenceAnnotation
persistent identity: annotation2507291
display id: annotation2507291
version: 1
top level: BBa_K1909004
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K1909004/annotation2507291/range2507291/1

https://synbiohub.org/public/igem/BBa_K1909004/annotation2507291/range2507291/1
type: Range
persistent identity: range2507291
display id: range2507291
version: 1
direction: direction/forward
top level: BBa_K1909004
start: 277
end: 279
orientation: inline

Changed CTG to CTA
type: SequenceAnnotation
persistent identity: annotation2507294
display id: annotation2507294
version: 1
top level: BBa_K1909004
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K1909004/annotation2507294/range2507294/1

https://synbiohub.org/public/igem/BBa_K1909004/annotation2507294/range2507294/1
type: Range
persistent identity: range2507294
display id: range2507294
version: 1
direction: direction/forward
top level: BBa_K1909004
start: 727
end: 729
orientation: inline

SP-AntiNIP
type: ComponentDefinition
persistent identity: BBa K157028
display id: BBa_K157028
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SP-AntiNIP-GGGSlinker-Tm-Fluolinker-cCFP
creator: iGEM Team Freiburg 2008
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Part consists of the signal peptide of Erbb1/EGF-receptor, scFV-antiNIP, GGGSflexlinker (extracellular part), the trans-membrane region of the Erbb1/EGF-receptor and the C-terminal part of the split fluorophor YFP with a fluolinker joined to its N-Terminus (intracellular part).
mutable notes: none
mutable provenance: Derived by cloning of the parts BBa_K157001, BBa_K157003, BBa_K157013, BBa_K157002, BBa_K157009 and BBa_K157005. For further information see the description of the parts mentioned above.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: Scar (Thr, Gly)
sequence annotation: C-Term_CFP
sequence annotation: Tm
sequence annotation: Fluolinker
sequence annotation: SP
sequence annotation: AgeI
sequence annotation: NgoMI
sequence annotation: Sc-Fv_anti-NIP
sequence annotation: GGGS-Linker
sequence annotation: SP-AntiNIP-GGGSlinker-Tm-Fluolinker-cCFP
sequence: https://synbiohub.org/public/igem/BBa_K157028_sequence/1

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993292
display id: annotation1993292
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157028/annotation1993292/range1993292/1

https://synbiohub.org/public/igem/BBa_K157028/annotation1993292/range1993292/1
type: Range
persistent identity: range1993292
display id: range1993292
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 823
end: 828
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993298
display id: annotation1993298
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157028/annotation1993298/range1993298/1

https://synbiohub.org/public/igem/BBa_K157028/annotation1993298/range1993298/1
type: Range
persistent identity: range1993298
display id: range1993298
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 1006
end: 1011
orientation: inline

C-Term_CFP
type: SequenceAnnotation
persistent identity: annotation1993299
display id: annotation1993299
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157028/annotation1993299/range1993299/1

https://synbiohub.org/public/igem/BBa_K157028/annotation1993299/range1993299/1
type: Range
persistent identity: range1993299
display id: range1993299
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 1012
end: 1254
orientation: inline

Tm
type: SequenceAnnotation
persistent identity: annotation1993295
display id: annotation1993295
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157028/annotation1993295/range1993295/1

https://synbiohub.org/public/igem/BBa_K157028/annotation1993295/range1993295/1
type: Range
persistent identity: range1993295
display id: range1993295
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 880
end: 948
orientation: inline

Fluolinker
type: SequenceAnnotation
persistent identity: annotation1993297
display id: annotation1993297
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157028/annotation1993297/range1993297/1

https://synbiohub.org/public/igem/BBa_K157028/annotation1993297/range1993297/1
type: Range
persistent identity: range1993297
display id: range1993297
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 955
end: 1005
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993294
display id: annotation1993294
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157028/annotation1993294/range1993294/1

https://synbiohub.org/public/igem/BBa_K157028/annotation1993294/range1993294/1
type: Range
persistent identity: range1993294
display id: range1993294
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 874
end: 879
orientation: inline

SP
type: SequenceAnnotation
persistent identity: annotation1993289
display id: annotation1993289
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157028/annotation1993289/range1993289/1

https://synbiohub.org/public/igem/BBa_K157028/annotation1993289/range1993289/1
type: Range
persistent identity: range1993289
display id: range1993289
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 10
end: 81
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation1990181
display id: annotation1990181
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157028/annotation1990181/range1990181/1

https://synbiohub.org/public/igem/BBa_K157028/annotation1990181/range1990181/1
type: Range
persistent identity: range1990181
display id: range1990181
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 1255
end: 1260
orientation: inline

NgoMI
type: SequenceAnnotation
persistent identity: annotation1990180
display id: annotation1990180
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157028/annotation1990180/range1990180/1

https://synbiohub.org/public/igem/BBa_K157028/annotation1990180/range1990180/1
type: Range
persistent identity: range1990180
display id: range1990180
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 4
end: 9
orientation: inline

Sc-Fv_anti-NIP
type: SequenceAnnotation
persistent identity: annotation1993291
display id: annotation1993291
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157028/annotation1993291/range1993291/1

https://synbiohub.org/public/igem/BBa_K157028/annotation1993291/range1993291/1
type: Range
persistent identity: range1993291
display id: range1993291
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 88
end: 822
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993296
display id: annotation1993296
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157028/annotation1993296/range1993296/1

https://synbiohub.org/public/igem/BBa_K157028/annotation1993296/range1993296/1
type: Range
persistent identity: range1993296
display id: range1993296
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 949
end: 954
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993290
display id: annotation1993290
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157028/annotation1993290/range1993290/1

https://synbiohub.org/public/igem/BBa_K157028/annotation1993290/range1993290/1
type: Range
persistent identity: range1993290
display id: range1993290
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 82
end: 87
orientation: inline

GGGS-Linker
type: SequenceAnnotation
persistent identity: annotation1993293
display id: annotation1993293
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157028/annotation1993293/range1993293/1

https://synbiohub.org/public/igem/BBa_K157028/annotation1993293/range1993293/1
type: Range
persistent identity: range1993293
display id: range1993293
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 829
end: 873
orientation: inline

SP-AntiNIP-GGGSlinker-Tm-Fluolinker-cCFP
type: SequenceAnnotation
persistent identity: annotation1990209
display id: annotation1990209
version: 1
top level: SP-AntiNIP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157028/annotation1990209/range1990209/1

https://synbiohub.org/public/igem/BBa_K157028/annotation1990209/range1990209/1
type: Range
persistent identity: range1990209
display id: range1990209
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 1
end: 1260
orientation: inline

BBa_K801031
type: ComponentDefinition
persistent identity: BBa K801031
display id: BBa_K801031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Phytochrome B (PhyB, first 908 N-terminal amino acids)
creator: Dong-Jiunn Jeffery TRUONG
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1057_
m user id: 0
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: improved version of BBa_K365002 to allow N- and C-terminal protein fusions using RFC 25
mutable notes: -
mutable provenance: Based on BBa_K365002
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: PhyB (2-908 AA) photosensitive core
sequence annotation: Essential Cys for chromophore attachement of phycocyanobilin/phytochromobilin
sequence annotation: Essential Arg for photoconvertible binding of PIF3/6
sequence annotation: Essential Gly for photoconvertible binding of PIF3/6
sequence: https://synbiohub.org/public/igem/BBa_K801031_sequence/1

PhyB (2-908 AA) photosensitive core
type: SequenceAnnotation
persistent identity: annotation2343344
display id: annotation2343344
version: 1
top level: BBa_K801031
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K801031/annotation2343344/range2343344/1

https://synbiohub.org/public/igem/BBa_K801031/annotation2343344/range2343344/1
type: Range
persistent identity: range2343344
display id: range2343344
version: 1
direction: direction/forward
top level: BBa_K801031
start: 1
end: 2721
orientation: inline

Essential Cys for chromophore attachement of phycocyanobilin/phytochromobilin
type: SequenceAnnotation
persistent identity: annotation2198864
display id: annotation2198864
version: 1
top level: BBa_K801031
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K801031/annotation2198864/range2198864/1

https://synbiohub.org/public/igem/BBa_K801031/annotation2198864/range2198864/1
type: Range
persistent identity: range2198864
display id: range2198864
version: 1
direction: direction/forward
top level: BBa_K801031
start: 1066
end: 1068
orientation: inline

Essential Arg for photoconvertible binding of PIF3/6
type: SequenceAnnotation
persistent identity: annotation2198862
display id: annotation2198862
version: 1
top level: BBa_K801031
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K801031/annotation2198862/range2198862/1

https://synbiohub.org/public/igem/BBa_K801031/annotation2198862/range2198862/1
type: Range
persistent identity: range2198862
display id: range2198862
version: 1
direction: direction/forward
top level: BBa_K801031
start: 1051
end: 1053
orientation: inline

Essential Gly for photoconvertible binding of PIF3/6
type: SequenceAnnotation
persistent identity: annotation2198859
display id: annotation2198859
version: 1
top level: BBa_K801031
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K801031/annotation2198859/range2198859/1

https://synbiohub.org/public/igem/BBa_K801031/annotation2198859/range2198859/1
type: Range
persistent identity: range2198859
display id: range2198859
version: 1
direction: direction/forward
top level: BBa_K801031
start: 328
end: 330
orientation: inline

Essential Gly for photoconvertible binding of PIF3/6
type: SequenceAnnotation
persistent identity: annotation2198861
display id: annotation2198861
version: 1
top level: BBa_K801031
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K801031/annotation2198861/range2198861/1

https://synbiohub.org/public/igem/BBa_K801031/annotation2198861/range2198861/1
type: Range
persistent identity: range2198861
display id: range2198861
version: 1
direction: direction/forward
top level: BBa_K801031
start: 1039
end: 1041
orientation: inline

Essential Arg for photoconvertible binding of PIF3/6
type: SequenceAnnotation
persistent identity: annotation2198856
display id: annotation2198856
version: 1
top level: BBa_K801031
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K801031/annotation2198856/range2198856/1

https://synbiohub.org/public/igem/BBa_K801031/annotation2198856/range2198856/1
type: Range
persistent identity: range2198856
display id: range2198856
version: 1
direction: direction/forward
top level: BBa_K801031
start: 325
end: 327
orientation: inline

SP-AntiNIP
type: ComponentDefinition
persistent identity: BBa K157026
display id: BBa_K157026
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SP-AntiNIP-GGGSlinker-Tm-Fluolinker-cYFP
creator: iGEM Team Freiburg 2008
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: A composite part consisting of the signal peptide of Erbb1/EGF-receptor, scFV-antiNIP, GGGSflexlinker (extracellular part), the trans-membrane region of the Erbb1/EGF-receptor and the C-terminal part of the split fluorophor YFP with a fluolinker joined to its N-Terminus (intracellular part).
mutable notes: none
mutable provenance: Derived by cloning of the parts BBa_K157001, BBa_K157003, BBa_K157013, BBa_K157002, BBa_K157009 and BBa_K157007. For further information see the description of the parts mentioned above.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: Scar (Thr, Gly)
sequence annotation: C-Term_YFP
sequence annotation: NgoMI
sequence annotation: AgeI
sequence annotation: SP-AntiNIP-GGGSlinker-Tm-Fluolinker-cYFP
sequence annotation: Fluolinker
sequence annotation: GGGS-Linker
sequence annotation: SP
sequence annotation: Sc-Fv_anti-NIP
sequence annotation: Tm
sequence: https://synbiohub.org/public/igem/BBa_K157026_sequence/1

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1992645
display id: annotation1992645
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157026/annotation1992645/range1992645/1

https://synbiohub.org/public/igem/BBa_K157026/annotation1992645/range1992645/1
type: Range
persistent identity: range1992645
display id: range1992645
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 949
end: 954
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1992643
display id: annotation1992643
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157026/annotation1992643/range1992643/1

https://synbiohub.org/public/igem/BBa_K157026/annotation1992643/range1992643/1
type: Range
persistent identity: range1992643
display id: range1992643
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 874
end: 879
orientation: inline

C-Term_YFP
type: SequenceAnnotation
persistent identity: annotation1992648
display id: annotation1992648
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157026/annotation1992648/range1992648/1

https://synbiohub.org/public/igem/BBa_K157026/annotation1992648/range1992648/1
type: Range
persistent identity: range1992648
display id: range1992648
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 1012
end: 1254
orientation: inline

NgoMI
type: SequenceAnnotation
persistent identity: annotation1990174
display id: annotation1990174
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157026/annotation1990174/range1990174/1

https://synbiohub.org/public/igem/BBa_K157026/annotation1990174/range1990174/1
type: Range
persistent identity: range1990174
display id: range1990174
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 4
end: 9
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation1990175
display id: annotation1990175
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157026/annotation1990175/range1990175/1

https://synbiohub.org/public/igem/BBa_K157026/annotation1990175/range1990175/1
type: Range
persistent identity: range1990175
display id: range1990175
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 1255
end: 1260
orientation: inline

SP-AntiNIP-GGGSlinker-Tm-Fluolinker-cYFP
type: SequenceAnnotation
persistent identity: annotation1990176
display id: annotation1990176
version: 1
top level: SP-AntiNIP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157026/annotation1990176/range1990176/1

https://synbiohub.org/public/igem/BBa_K157026/annotation1990176/range1990176/1
type: Range
persistent identity: range1990176
display id: range1990176
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 1
end: 1260
orientation: inline

Fluolinker
type: SequenceAnnotation
persistent identity: annotation1992646
display id: annotation1992646
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157026/annotation1992646/range1992646/1

https://synbiohub.org/public/igem/BBa_K157026/annotation1992646/range1992646/1
type: Range
persistent identity: range1992646
display id: range1992646
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 955
end: 1005
orientation: inline

GGGS-Linker
type: SequenceAnnotation
persistent identity: annotation1992642
display id: annotation1992642
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157026/annotation1992642/range1992642/1

https://synbiohub.org/public/igem/BBa_K157026/annotation1992642/range1992642/1
type: Range
persistent identity: range1992642
display id: range1992642
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 829
end: 873
orientation: inline

SP
type: SequenceAnnotation
persistent identity: annotation1992638
display id: annotation1992638
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157026/annotation1992638/range1992638/1

https://synbiohub.org/public/igem/BBa_K157026/annotation1992638/range1992638/1
type: Range
persistent identity: range1992638
display id: range1992638
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 10
end: 81
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1992647
display id: annotation1992647
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157026/annotation1992647/range1992647/1

https://synbiohub.org/public/igem/BBa_K157026/annotation1992647/range1992647/1
type: Range
persistent identity: range1992647
display id: range1992647
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 1006
end: 1011
orientation: inline

Sc-Fv_anti-NIP
type: SequenceAnnotation
persistent identity: annotation1992640
display id: annotation1992640
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157026/annotation1992640/range1992640/1

https://synbiohub.org/public/igem/BBa_K157026/annotation1992640/range1992640/1
type: Range
persistent identity: range1992640
display id: range1992640
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 88
end: 822
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1992639
display id: annotation1992639
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157026/annotation1992639/range1992639/1

https://synbiohub.org/public/igem/BBa_K157026/annotation1992639/range1992639/1
type: Range
persistent identity: range1992639
display id: range1992639
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 82
end: 87
orientation: inline

Tm
type: SequenceAnnotation
persistent identity: annotation1992644
display id: annotation1992644
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157026/annotation1992644/range1992644/1

https://synbiohub.org/public/igem/BBa_K157026/annotation1992644/range1992644/1
type: Range
persistent identity: range1992644
display id: range1992644
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 880
end: 948
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1992641
display id: annotation1992641
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157026/annotation1992641/range1992641/1

https://synbiohub.org/public/igem/BBa_K157026/annotation1992641/range1992641/1
type: Range
persistent identity: range1992641
display id: range1992641
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 823
end: 828
orientation: inline

flyPCD
type: ComponentDefinition
persistent identity: BBa J176001
display id: BBa_J176001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: flyPCD
creator: Karmella Haynes
created: 2011-07-01T11:00:00Z
modified: 2015-08-31T04:08:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _863_
m user id: 0
owner id: 1144
owning group id: 61
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Amino acids 2-64 from the zebrafish CBX2 protein. This is the conserved Polycomb chromodomain predicted to bind trimethylated histone H3 lysine 27 in chromatin.
mutable notes: Codons were optimized for expression in cultured human cells.
mutable provenance: Danio rerio (zebrafish) CBX2 amino acids 2-64. The human codon-optimized coding sequence was synthesized by Genscript.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_J176001_sequence/1

BBa_J18951
type: ComponentDefinition
persistent identity: BBa J18951
display id: BBa_J18951
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FRB ~ mCitrine
creator: Raik Gruenberg
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
discontinued: false
dominant: false
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: P19 expression cassette from Gruenberg et al. 2010 series of interaction reporter proteins. All proteins share the same architecture: * T7Start-domain1-5xGS-domain2-preSCsite-HisTag-T7Stop Only Domains1 and 2 vary and are taken from a list of protein interaction input and output (readout) parts. ===Expression=== Proteins were expressed in E. coli BL21 using T7 Polymerase and were purified by His-tag affinity. ===Reference=== * Gruenberg, Ferrar, van der Sloot, Serrano (2010): Building Blocks for Protein Interaction Devices
mutable notes: Constructs were assembled according to R.F.C. 25. All parts were codon optimized for E. coli.
mutable provenance: synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0105
component: BBa_J18913
component: BBa_J18919
component: BBa_J18912
component: BBa_J18926
component: BBa_J18931
component: BBa_J18922
component: BBa_J18909
sequence annotation: BBa_B0105
sequence annotation: BBa_J18912
sequence annotation: BBa_J18913
sequence annotation: BBa_J18909
sequence annotation: BBa_J18919
sequence annotation: BBa_J18926
sequence annotation: BBa_J18922
sequence annotation: BBa_J18931
sequence: https://synbiohub.org/public/igem/BBa_J18951_sequence/1

BBa_B0105
type: Component
persistent identity: component2066784
display id: component2066784
version: 1
top level: BBa_J18951
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_J18913
type: Component
persistent identity: component2066802
display id: component2066802
version: 1
top level: BBa_J18951
access: public
definition: https://synbiohub.org/public/igem/BBa_J18913/1

BBa_B0105
type: Component
persistent identity: component2066790
display id: component2066790
version: 1
top level: BBa_J18951
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_B0105
type: Component
persistent identity: component2066796
display id: component2066796
version: 1
top level: BBa_J18951
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_B0105
type: Component
persistent identity: component2066793
display id: component2066793
version: 1
top level: BBa_J18951
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_J18919
type: Component
persistent identity: component2066794
display id: component2066794
version: 1
top level: BBa_J18951
access: public
definition: https://synbiohub.org/public/igem/BBa_J18919/1

BBa_B0105
type: Component
persistent identity: component2066787
display id: component2066787
version: 1
top level: BBa_J18951
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_J18912
type: Component
persistent identity: component2066782
display id: component2066782
version: 1
top level: BBa_J18951
access: public
definition: https://synbiohub.org/public/igem/BBa_J18912/1

BBa_J18926
type: Component
persistent identity: component2066785
display id: component2066785
version: 1
top level: BBa_J18951
access: public
definition: FRB

BBa_J18931
type: Component
persistent identity: component2066791
display id: component2066791
version: 1
top level: BBa_J18951
access: public
definition: https://synbiohub.org/public/igem/BBa_J18931/1

BBa_J18922
type: Component
persistent identity: component2066788
display id: component2066788
version: 1
top level: BBa_J18951
access: public
definition: https://synbiohub.org/public/igem/BBa_J18922/1

BBa_B0105
type: Component
persistent identity: component2066800
display id: component2066800
version: 1
top level: BBa_J18951
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_J18909
type: Component
persistent identity: component2066798
display id: component2066798
version: 1
top level: BBa_J18951
access: public
definition: https://synbiohub.org/public/igem/BBa_J18909/1

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066800
display id: annotation2066800
version: 1
top level: BBa_J18951
location: https://synbiohub.org/public/igem/BBa_J18951/annotation2066800/range2066800/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18951/annotation2066800/range2066800/1
type: Range
persistent identity: range2066800
display id: range2066800
version: 1
direction: direction/reverse
top level: BBa_J18951
start: 1179
end: 1184
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066793
display id: annotation2066793
version: 1
top level: BBa_J18951
location: https://synbiohub.org/public/igem/BBa_J18951/annotation2066793/range2066793/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18951/annotation2066793/range2066793/1
type: Range
persistent identity: range2066793
display id: range2066793
version: 1
direction: direction/reverse
top level: BBa_J18951
start: 1125
end: 1130
orientation: inline

BBa_J18912
type: SequenceAnnotation
persistent identity: annotation2066782
display id: annotation2066782
version: 1
top level: BBa_J18951
location: https://synbiohub.org/public/igem/BBa_J18951/annotation2066782/range2066782/1
component: BBa_J18912

https://synbiohub.org/public/igem/BBa_J18951/annotation2066782/range2066782/1
type: Range
persistent identity: range2066782
display id: range2066782
version: 1
direction: direction/reverse
top level: BBa_J18951
start: 1
end: 83
orientation: inline

BBa_J18913
type: SequenceAnnotation
persistent identity: annotation2066802
display id: annotation2066802
version: 1
top level: BBa_J18951
location: https://synbiohub.org/public/igem/BBa_J18951/annotation2066802/range2066802/1
component: BBa_J18913

https://synbiohub.org/public/igem/BBa_J18951/annotation2066802/range2066802/1
type: Range
persistent identity: range2066802
display id: range2066802
version: 1
direction: direction/reverse
top level: BBa_J18951
start: 1185
end: 1319
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066796
display id: annotation2066796
version: 1
top level: BBa_J18951
location: https://synbiohub.org/public/igem/BBa_J18951/annotation2066796/range2066796/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18951/annotation2066796/range2066796/1
type: Range
persistent identity: range2066796
display id: range2066796
version: 1
direction: direction/reverse
top level: BBa_J18951
start: 1155
end: 1160
orientation: inline

BBa_J18909
type: SequenceAnnotation
persistent identity: annotation2066798
display id: annotation2066798
version: 1
top level: BBa_J18951
location: https://synbiohub.org/public/igem/BBa_J18951/annotation2066798/range2066798/1
component: BBa_J18909

https://synbiohub.org/public/igem/BBa_J18951/annotation2066798/range2066798/1
type: Range
persistent identity: range2066798
display id: range2066798
version: 1
direction: direction/reverse
top level: BBa_J18951
start: 1161
end: 1178
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066787
display id: annotation2066787
version: 1
top level: BBa_J18951
location: https://synbiohub.org/public/igem/BBa_J18951/annotation2066787/range2066787/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18951/annotation2066787/range2066787/1
type: Range
persistent identity: range2066787
display id: range2066787
version: 1
direction: direction/reverse
top level: BBa_J18951
start: 369
end: 374
orientation: inline

BBa_J18919
type: SequenceAnnotation
persistent identity: annotation2066794
display id: annotation2066794
version: 1
top level: BBa_J18951
location: https://synbiohub.org/public/igem/BBa_J18951/annotation2066794/range2066794/1
component: BBa_J18919

https://synbiohub.org/public/igem/BBa_J18951/annotation2066794/range2066794/1
type: Range
persistent identity: range2066794
display id: range2066794
version: 1
direction: direction/reverse
top level: BBa_J18951
start: 1131
end: 1154
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066784
display id: annotation2066784
version: 1
top level: BBa_J18951
location: https://synbiohub.org/public/igem/BBa_J18951/annotation2066784/range2066784/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18951/annotation2066784/range2066784/1
type: Range
persistent identity: range2066784
display id: range2066784
version: 1
direction: direction/reverse
top level: BBa_J18951
start: 84
end: 89
orientation: inline

BBa_J18926
type: SequenceAnnotation
persistent identity: annotation2066785
display id: annotation2066785
version: 1
top level: BBa_J18951
location: https://synbiohub.org/public/igem/BBa_J18951/annotation2066785/range2066785/1
component: BBa_J18926

https://synbiohub.org/public/igem/BBa_J18951/annotation2066785/range2066785/1
type: Range
persistent identity: range2066785
display id: range2066785
version: 1
direction: direction/reverse
top level: BBa_J18951
start: 90
end: 368
orientation: inline

BBa_J18922
type: SequenceAnnotation
persistent identity: annotation2066788
display id: annotation2066788
version: 1
top level: BBa_J18951
location: https://synbiohub.org/public/igem/BBa_J18951/annotation2066788/range2066788/1
component: BBa_J18922

https://synbiohub.org/public/igem/BBa_J18951/annotation2066788/range2066788/1
type: Range
persistent identity: range2066788
display id: range2066788
version: 1
direction: direction/reverse
top level: BBa_J18951
start: 375
end: 404
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066790
display id: annotation2066790
version: 1
top level: BBa_J18951
location: https://synbiohub.org/public/igem/BBa_J18951/annotation2066790/range2066790/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18951/annotation2066790/range2066790/1
type: Range
persistent identity: range2066790
display id: range2066790
version: 1
direction: direction/reverse
top level: BBa_J18951
start: 405
end: 410
orientation: inline

BBa_J18931
type: SequenceAnnotation
persistent identity: annotation2066791
display id: annotation2066791
version: 1
top level: BBa_J18951
location: https://synbiohub.org/public/igem/BBa_J18951/annotation2066791/range2066791/1
component: BBa_J18931

https://synbiohub.org/public/igem/BBa_J18951/annotation2066791/range2066791/1
type: Range
persistent identity: range2066791
display id: range2066791
version: 1
direction: direction/reverse
top level: BBa_J18951
start: 411
end: 1124
orientation: inline

SP-AntiNIP
type: ComponentDefinition
persistent identity: BBa K157024
display id: BBa_K157024
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SP-AntiNIP-GGGSlinker-Tm-betaLactamase(Bla1)
creator: iGEM Team Freiburg 2008
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: A composite part consisting of the signal-peptide of Erbb1/EGF-receptor, scFV-antiNIP, GGGSflexlinker (extracellular part), the trans-membrane region of the Erbb1/EGF-receptor and the N-terminal part of split-enzyme beta-Lactamase (intracellular part).
mutable notes: none
mutable provenance: Derived by cloning of the parts BBa_K157001, BBa_K157003, BBa_K157013, BBa_K157002 and BBa_I757011. For further information see the description of the parts mentioned above.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: NgoMI
sequence annotation: Bla1
sequence annotation: Tm
sequence annotation: Scar (Thr, Gly)
sequence annotation: SP
sequence annotation: Sc-Fv_anti-NIP
sequence annotation: SP-AntiNIP-GGGSlinker-Tm-betaLactamase(Bla1)
sequence annotation: AgeI
sequence annotation: GGGS-Linker
sequence: https://synbiohub.org/public/igem/BBa_K157024_sequence/1

NgoMI
type: SequenceAnnotation
persistent identity: annotation1990166
display id: annotation1990166
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157024/annotation1990166/range1990166/1

https://synbiohub.org/public/igem/BBa_K157024/annotation1990166/range1990166/1
type: Range
persistent identity: range1990166
display id: range1990166
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 4
end: 9
orientation: inline

Bla1
type: SequenceAnnotation
persistent identity: annotation1992613
display id: annotation1992613
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157024/annotation1992613/range1992613/1

https://synbiohub.org/public/igem/BBa_K157024/annotation1992613/range1992613/1
type: Range
persistent identity: range1992613
display id: range1992613
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 955
end: 1479
orientation: inline

Tm
type: SequenceAnnotation
persistent identity: annotation1992611
display id: annotation1992611
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157024/annotation1992611/range1992611/1

https://synbiohub.org/public/igem/BBa_K157024/annotation1992611/range1992611/1
type: Range
persistent identity: range1992611
display id: range1992611
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 880
end: 948
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1992610
display id: annotation1992610
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157024/annotation1992610/range1992610/1

https://synbiohub.org/public/igem/BBa_K157024/annotation1992610/range1992610/1
type: Range
persistent identity: range1992610
display id: range1992610
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 874
end: 879
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1992606
display id: annotation1992606
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157024/annotation1992606/range1992606/1

https://synbiohub.org/public/igem/BBa_K157024/annotation1992606/range1992606/1
type: Range
persistent identity: range1992606
display id: range1992606
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 823
end: 828
orientation: inline

SP
type: SequenceAnnotation
persistent identity: annotation1992602
display id: annotation1992602
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157024/annotation1992602/range1992602/1

https://synbiohub.org/public/igem/BBa_K157024/annotation1992602/range1992602/1
type: Range
persistent identity: range1992602
display id: range1992602
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 10
end: 81
orientation: inline

Sc-Fv_anti-NIP
type: SequenceAnnotation
persistent identity: annotation1992604
display id: annotation1992604
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157024/annotation1992604/range1992604/1

https://synbiohub.org/public/igem/BBa_K157024/annotation1992604/range1992604/1
type: Range
persistent identity: range1992604
display id: range1992604
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 88
end: 822
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1992612
display id: annotation1992612
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157024/annotation1992612/range1992612/1

https://synbiohub.org/public/igem/BBa_K157024/annotation1992612/range1992612/1
type: Range
persistent identity: range1992612
display id: range1992612
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 949
end: 954
orientation: inline

SP-AntiNIP-GGGSlinker-Tm-betaLactamase(Bla1)
type: SequenceAnnotation
persistent identity: annotation1990168
display id: annotation1990168
version: 1
top level: SP-AntiNIP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157024/annotation1990168/range1990168/1

https://synbiohub.org/public/igem/BBa_K157024/annotation1990168/range1990168/1
type: Range
persistent identity: range1990168
display id: range1990168
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 1
end: 1485
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation1990167
display id: annotation1990167
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157024/annotation1990167/range1990167/1

https://synbiohub.org/public/igem/BBa_K157024/annotation1990167/range1990167/1
type: Range
persistent identity: range1990167
display id: range1990167
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 1480
end: 1485
orientation: inline

GGGS-Linker
type: SequenceAnnotation
persistent identity: annotation1992608
display id: annotation1992608
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157024/annotation1992608/range1992608/1

https://synbiohub.org/public/igem/BBa_K157024/annotation1992608/range1992608/1
type: Range
persistent identity: range1992608
display id: range1992608
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 829
end: 873
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1992603
display id: annotation1992603
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157024/annotation1992603/range1992603/1

https://synbiohub.org/public/igem/BBa_K157024/annotation1992603/range1992603/1
type: Range
persistent identity: range1992603
display id: range1992603
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 82
end: 87
orientation: inline

FRB
type: ComponentDefinition
persistent identity: BBa J18926
display id: BBa_J18926
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FRB(T2098L)
creator: Raik Gruenberg
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Modified FKBP12-rapamycin-binding domain (FRB) of human mTOR Kinase (=mammalian Target of Rapamycin, also called FRAP = FKBP12-rapamycin associated protein). In presence of the immunosupressant drug rapamycin, FRB forms a high-affinity complex with FKBP12. There is no detectable FKBP12 - FRB interaction in absence of rapamycin. ===Modification for a non-toxic dimerizer=== Rapamycin arrests growth and proliferation of many cell types (reviewed in Jacinto and Hall, 2003) by inhibiting protein translation. The mutation T2089L (mTor numbering) makes FRB binding to a non-toxic Rapamycin analogue (rapalogue) AP21967 as well as the original Rapamycin. The FRB - FKBP12 heterodimerization can then be triggered by both Rapamycin and the non-toxic rapalogue. This variant of FRB and the synthetic dimerizer AP21967 are the basis of the Argent(tm) heterodimerization kit from Ariad Pharmaceuticals Inc. '''Note:''' Two additional mutations would convert FRB into FRB* which is used for conditional (drug-reversible) degradation of fusion proteins. These two additional mutations are not implemented in this part. ===Protein Parameters:=== * Number of amino acids: 93 * Molecular weight: 11285.9 * Theoretical pI: 6.47
mutable notes: * aa translation from human mTOR, * mutation T2089L (verified against pC4-RHE from Ariad) * GeneArt codon optimization for E. coli * gene synthesis
mutable provenance: human mTOR (aa sequence) gene synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_J18926_sequence/1

BBa_K801035
type: ComponentDefinition
persistent identity: BBa K801035
display id: BBa_K801035
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Linker-PIF3
creator: Dong-Jiunn Jeffery TRUONG
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
group u list: _1057_
m user id: 0
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: composite part of Bba_K365005 and Bba_K365000
mutable notes: -
mutable provenance: composite part of Bba_K365005 and Bba_K365000
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: RFC25 fusion scar
sequence annotation: BBa_K365000
sequence annotation: T > A
sequence annotation: BBa_K365005
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K801035_sequence/1

RFC25 fusion scar
type: SequenceAnnotation
persistent identity: annotation2198904
display id: annotation2198904
version: 1
top level: BBa_K801035
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801035/annotation2198904/range2198904/1

https://synbiohub.org/public/igem/BBa_K801035/annotation2198904/range2198904/1
type: Range
persistent identity: range2198904
display id: range2198904
version: 1
direction: direction/forward
top level: BBa_K801035
start: 61
end: 66
orientation: inline

BBa_K365000
type: SequenceAnnotation
persistent identity: annotation2190058
display id: annotation2190058
version: 1
top level: BBa_K801035
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801035/annotation2190058/range2190058/1

https://synbiohub.org/public/igem/BBa_K801035/annotation2190058/range2190058/1
type: Range
persistent identity: range2190058
display id: range2190058
version: 1
direction: direction/reverse
top level: BBa_K801035
start: 67
end: 366
orientation: inline

T > A
type: SequenceAnnotation
persistent identity: annotation2190057
display id: annotation2190057
version: 1
top level: BBa_K801035
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K801035/annotation2190057/range2190057/1

https://synbiohub.org/public/igem/BBa_K801035/annotation2190057/range2190057/1
type: Range
persistent identity: range2190057
display id: range2190057
version: 1
direction: direction/forward
top level: BBa_K801035
start: 243
end: 243
orientation: inline

BBa_K365005
type: SequenceAnnotation
persistent identity: annotation2190055
display id: annotation2190055
version: 1
top level: BBa_K801035
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801035/annotation2190055/range2190055/1

https://synbiohub.org/public/igem/BBa_K801035/annotation2190055/range2190055/1
type: Range
persistent identity: range2190055
display id: range2190055
version: 1
direction: direction/reverse
top level: BBa_K801035
start: 1
end: 60
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2190056
display id: annotation2190056
version: 1
top level: BBa_K801035
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K801035/annotation2190056/range2190056/1

https://synbiohub.org/public/igem/BBa_K801035/annotation2190056/range2190056/1
type: Range
persistent identity: range2190056
display id: range2190056
version: 1
direction: direction/forward
top level: BBa_K801035
start: 67
end: 69
orientation: inline

FKBP12
type: ComponentDefinition
persistent identity: BBa J18925
display id: BBa_J18925
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FKBP12
creator: Raik Gruenberg
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The immunophilin protein FKBP12 (FK506-binding protein) is the target of the natural compounds FK506 and rapamycin. The FKBP/rapamycin or FKBP/FK506 complex then binds to several other proteins and suppresses immune reactions. Drug screening and design has turned up a selection of other small molecules that bind with high specificity to FKBP12 itself or to mutants of FKBP12. A second human protein, FRB is among the most prominent binding targets of the FKBP/rapamycin complex (using the "other face" of the rapamycin molecule). Rapamycin can herefore induce the hetero-dimerization of FKBP12 and FRB. == See also == * structure of FRB / FKBP / Rapamycin complex: PDB 1nsg (CRG-internal ID: rg0073)
mutable notes: * gene synthesis * codon optimized for E. coli
mutable provenance: literature
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_J18925_sequence/1

SP-AntiNIP
type: ComponentDefinition
persistent identity: BBa K157030
display id: BBa_K157030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SP-AntiNIP-GGGSlinker-Tm-Splitluciferase2/2
creator: iGEM Team Freiburg 2008
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: true
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Part consists of the signal peptide of Erbb1/EGF-receptor, scFV-antiNIP, GGGSflexlinker (extracellular part), the trans-membrane region of the Erbb1/EGF-receptor and the C-terminal part of the split fluorophor YFP with a fluolinker joined to its N-Terminus (intracellular part).
mutable notes: none
mutable provenance: Derived by cloning of the parts BBa_K157001, BBa_K157003, BBa_K157013, BBa_K157002 and Part one of Split- Luciferase For further information see the description of the parts mentioned above.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: Scar (Thr, Gly)
sequence annotation: SP
sequence annotation: Splitluciferase2/2
sequence annotation: Tm
sequence annotation: NgoMI
sequence annotation: AgeI
sequence annotation: SP-AntiNIP-GGGSlinker-Tm-Splitluciferase2/2
sequence annotation: GGGS-Linker
sequence annotation: Sc-Fv_anti-NIP
sequence: https://synbiohub.org/public/igem/BBa_K157030_sequence/1

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993314
display id: annotation1993314
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157030/annotation1993314/range1993314/1

https://synbiohub.org/public/igem/BBa_K157030/annotation1993314/range1993314/1
type: Range
persistent identity: range1993314
display id: range1993314
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 874
end: 879
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993312
display id: annotation1993312
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157030/annotation1993312/range1993312/1

https://synbiohub.org/public/igem/BBa_K157030/annotation1993312/range1993312/1
type: Range
persistent identity: range1993312
display id: range1993312
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 823
end: 828
orientation: inline

SP
type: SequenceAnnotation
persistent identity: annotation1993309
display id: annotation1993309
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157030/annotation1993309/range1993309/1

https://synbiohub.org/public/igem/BBa_K157030/annotation1993309/range1993309/1
type: Range
persistent identity: range1993309
display id: range1993309
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 10
end: 81
orientation: inline

Splitluciferase2/2
type: SequenceAnnotation
persistent identity: annotation1993317
display id: annotation1993317
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157030/annotation1993317/range1993317/1

https://synbiohub.org/public/igem/BBa_K157030/annotation1993317/range1993317/1
type: Range
persistent identity: range1993317
display id: range1993317
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 955
end: 1230
orientation: inline

Tm
type: SequenceAnnotation
persistent identity: annotation1993315
display id: annotation1993315
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157030/annotation1993315/range1993315/1

https://synbiohub.org/public/igem/BBa_K157030/annotation1993315/range1993315/1
type: Range
persistent identity: range1993315
display id: range1993315
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 880
end: 948
orientation: inline

NgoMI
type: SequenceAnnotation
persistent identity: annotation1990263
display id: annotation1990263
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157030/annotation1990263/range1990263/1

https://synbiohub.org/public/igem/BBa_K157030/annotation1990263/range1990263/1
type: Range
persistent identity: range1990263
display id: range1990263
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 4
end: 9
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993316
display id: annotation1993316
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157030/annotation1993316/range1993316/1

https://synbiohub.org/public/igem/BBa_K157030/annotation1993316/range1993316/1
type: Range
persistent identity: range1993316
display id: range1993316
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 949
end: 954
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation1990264
display id: annotation1990264
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157030/annotation1990264/range1990264/1

https://synbiohub.org/public/igem/BBa_K157030/annotation1990264/range1990264/1
type: Range
persistent identity: range1990264
display id: range1990264
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 1231
end: 1236
orientation: inline

SP-AntiNIP-GGGSlinker-Tm-Splitluciferase2/2
type: SequenceAnnotation
persistent identity: annotation1990265
display id: annotation1990265
version: 1
top level: SP-AntiNIP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157030/annotation1990265/range1990265/1

https://synbiohub.org/public/igem/BBa_K157030/annotation1990265/range1990265/1
type: Range
persistent identity: range1990265
display id: range1990265
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 1
end: 1236
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993310
display id: annotation1993310
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157030/annotation1993310/range1993310/1

https://synbiohub.org/public/igem/BBa_K157030/annotation1993310/range1993310/1
type: Range
persistent identity: range1993310
display id: range1993310
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 82
end: 87
orientation: inline

GGGS-Linker
type: SequenceAnnotation
persistent identity: annotation1993313
display id: annotation1993313
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157030/annotation1993313/range1993313/1

https://synbiohub.org/public/igem/BBa_K157030/annotation1993313/range1993313/1
type: Range
persistent identity: range1993313
display id: range1993313
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 829
end: 873
orientation: inline

Sc-Fv_anti-NIP
type: SequenceAnnotation
persistent identity: annotation1993311
display id: annotation1993311
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157030/annotation1993311/range1993311/1

https://synbiohub.org/public/igem/BBa_K157030/annotation1993311/range1993311/1
type: Range
persistent identity: range1993311
display id: range1993311
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 88
end: 822
orientation: inline

BBa_K404314
type: ComponentDefinition
persistent identity: BBa K404314
display id: BBa_K404314
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: DARPin-E01
creator: Freiburg Bioware 2010
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 6733
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: DARPin-E01
sequence: https://synbiohub.org/public/igem/BBa_K404314_sequence/1

DARPin-E01
type: SequenceAnnotation
persistent identity: annotation2094760
display id: annotation2094760
version: 1
top level: BBa_K404314
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404314/annotation2094760/range2094760/1

https://synbiohub.org/public/igem/BBa_K404314/annotation2094760/range2094760/1
type: Range
persistent identity: range2094760
display id: range2094760
version: 1
direction: direction/forward
top level: BBa_K404314
start: 1
end: 459
orientation: inline

BBa_J18944
type: ComponentDefinition
persistent identity: BBa J18944
display id: BBa_J18944
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ZipE34 ~ mCerulean
creator: Raik Gruenberg
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
discontinued: false
dominant: false
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: P12 expression cassette from Gruenberg et al. 2010 series of interaction reporter proteins. All proteins share the same architecture: * T7Start-domain1-5xGS-domain2-preSCsite-HisTag-T7Stop Only Domains1 and 2 vary and are taken from a list of protein interaction input and output (readout) parts. ===Expression=== Proteins were expressed in E. coli BL21 using T7 Polymerase and were purified by His-tag affinity. ===Reference=== * Gruenberg, Ferrar, van der Sloot, Serrano (2010): Building Blocks for Protein Interaction Devices
mutable notes: Constructs were assembled according to R.F.C. 25. All parts were codon optimized for E. coli.
mutable provenance: synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0105
component: BBa_J18930
component: BBa_J18909
component: BBa_J18913
component: BBa_J18923
component: BBa_J18912
component: BBa_J18922
component: BBa_J18919
sequence annotation: BBa_J18919
sequence annotation: BBa_J18913
sequence annotation: BBa_J18922
sequence annotation: BBa_B0105
sequence annotation: BBa_J18909
sequence annotation: BBa_J18930
sequence annotation: BBa_J18912
sequence annotation: BBa_J18923
sequence: https://synbiohub.org/public/igem/BBa_J18944_sequence/1

BBa_B0105
type: Component
persistent identity: component2066639
display id: component2066639
version: 1
top level: BBa_J18944
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_B0105
type: Component
persistent identity: component2066636
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GLucFrag1
type: ComponentDefinition
persistent identity: BBa J18928
display id: BBa_J18928
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Gaussia princeps Luciferase fragment 1
creator: Raik Gruenberg
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: N-terminal fragment of H. gaussia luciferase for PCA. '''Disulfide bonds:''' * likely, many Cys in sequence ===Purification=== Inouye & Sahara (2008) discuss the purification and in-vitro activity of different Luciferases, including Gaussia. They indicate that Gaussia Luciferase was previously purified from insoluable fractions -- perhaps due to the high Cys content. Goerke et al (2008) Provide optimized in-vitro expression conditions for Gaussia Luciferase based on tuning the strain from which the cell-free lysate is produced. ===References=== http://www.ncbi.nlm.nih.gov/pubmed/17099704 Remy I, Michnick SW. (2006) A highly sensitive protein-protein interaction assay based on Gaussia luciferase. http://nar.oxfordjournals.org/cgi/content/full/27/13/e4#hd1 Maroun M, Aronheim A. (2007) A novel in vivo assay for the analysis of protein-protein interaction. PMID: 10373602 http://www.ncbi.nlm.nih.gov/pubmed/19373229 Tannous BA. (2009) Gaussia luciferase reporter assay for monitoring biological processes in culture and in vivo. http://www.ncbi.nlm.nih.gov/pubmed/18789309 Inouye S, Sahara Y. (2008) Soluble protein expression in E. coli cells using IgG-binding domain of protein A as a solubilizing partner in the cold induced system. http://www.ncbi.nlm.nih.gov/pubmed/18555198 Goerke AR, Loening AM, Gambhir SS, Swartz JR. (2008) Cell-free metabolic engineering promotes high-level production of bioactive Gaussia princeps luciferase.
mutable notes: * gene synthesis * codon optimized for E. coli
mutable provenance: gene synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_J18928_sequence/1

BBa_J18938
type: ComponentDefinition
persistent identity: BBa J18938
display id: BBa_J18938
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FKBP ~ bla-frag1
creator: Raik Gruenberg
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
discontinued: false
dominant: false
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: P06 expression cassette from Gruenberg et al. 2010 series of interaction reporter proteins. All proteins share the same architecture: * T7Start-domain1-5xGS-domain2-preSCsite-HisTag-T7Stop Only Domains1 and 2 vary and are taken from a list of protein interaction input and output (readout) parts. ===Expression=== Proteins were expressed in E. coli BL21 using T7 Polymerase and were purified by His-tag affinity. ===Reference=== * Gruenberg, Ferrar, van der Sloot, Serrano (2010): Building Blocks for Protein Interaction Devices
mutable notes: Constructs were assembled according to R.F.C. 25. All parts were codon optimized for E. coli.
mutable provenance: synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
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persistent identity: BBa K404303
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version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Z-EGFR-1907_Short-Linker
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: Short-linker
sequence annotation: Z-EGFR-1907
sequence: https://synbiohub.org/public/igem/BBa_K404303_sequence/1

Short-linker
type: SequenceAnnotation
persistent identity: annotation2094743
display id: annotation2094743
version: 1
top level: BBa_K404303
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404303/annotation2094743/range2094743/1

https://synbiohub.org/public/igem/BBa_K404303/annotation2094743/range2094743/1
type: Range
persistent identity: range2094743
display id: range2094743
version: 1
direction: direction/forward
top level: BBa_K404303
start: 181
end: 192
orientation: inline

Z-EGFR-1907
type: SequenceAnnotation
persistent identity: annotation2094742
display id: annotation2094742
version: 1
top level: BBa_K404303
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404303/annotation2094742/range2094742/1

https://synbiohub.org/public/igem/BBa_K404303/annotation2094742/range2094742/1
type: Range
persistent identity: range2094742
display id: range2094742
version: 1
direction: direction/forward
top level: BBa_K404303
start: 1
end: 175
orientation: inline

BBa_K1478001
type: ComponentDefinition
persistent identity: BBa K1478001
display id: BBa_K1478001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cellulose binding domain
creator: Fabian Fr??mling
created: 2014-09-12T11:00:00Z
modified: 2015-06-17T01:26:18Z
discontinued: false
dominant: false
group u list: _1858_
m user id: 4206
owner id: 20973
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Coding region for cellulose binding domain. When fused to a protein, protein attaches to cellulose
mutable notes: Sequence was optimized for Escherichia coli and Arabidopsis thaliana codon usage.
mutable provenance: Domain derived from cellulose binding protein from Chlostridium cellulovorans(WP_013291799).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K1478001_sequence/1

BBa_K801041
type: ComponentDefinition
persistent identity: BBa K801041
display id: BBa_K801041
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SV40NLS-PhyB-linker-LexA (part for a LexA based light-switchable promoter system)
creator: Dong-Jiunn Jeffery TRUONG
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1057_
m user id: 0
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Composite part of BBa_K801030, BBa_K801030, BBa_K365005, BBa_K801033
mutable notes: -
mutable provenance: Composite part of BBa_K801030, BBa_K801030, BBa_K365005, BBa_K801033
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: RFC25 fusion scar
sequence annotation: SV40 nuclear localization sequence (recognized by importin alpha)
sequence annotation: BBa_K801030
sequence annotation: BBa_K801036
sequence annotation: Essential Gly for photoconvertible binding of PIF3
sequence annotation: Essential Cys for chromophore attachement of phycocyanobilin (PCB)
sequence annotation: Essential Arg for photoconvertible binding of PIF3
sequence annotation: BBa_K801031
sequence annotation: BBa_K801033
sequence annotation: BBa_K365005
sequence: https://synbiohub.org/public/igem/BBa_K801041_sequence/1

RFC25 fusion scar
type: SequenceAnnotation
persistent identity: annotation2199722
display id: annotation2199722
version: 1
top level: BBa_K801041
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801041/annotation2199722/range2199722/1

https://synbiohub.org/public/igem/BBa_K801041/annotation2199722/range2199722/1
type: Range
persistent identity: range2199722
display id: range2199722
version: 1
direction: direction/forward
top level: BBa_K801041
start: 58
end: 63
orientation: inline

SV40 nuclear localization sequence (recognized by importin alpha)
type: SequenceAnnotation
persistent identity: annotation2199707
display id: annotation2199707
version: 1
top level: BBa_K801041
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801041/annotation2199707/range2199707/1

https://synbiohub.org/public/igem/BBa_K801041/annotation2199707/range2199707/1
type: Range
persistent identity: range2199707
display id: range2199707
version: 1
direction: direction/forward
top level: BBa_K801041
start: 22
end: 42
orientation: inline

BBa_K801030
type: SequenceAnnotation
persistent identity: annotation2199708
display id: annotation2199708
version: 1
top level: BBa_K801041
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801041/annotation2199708/range2199708/1

https://synbiohub.org/public/igem/BBa_K801041/annotation2199708/range2199708/1
type: Range
persistent identity: range2199708
display id: range2199708
version: 1
direction: direction/forward
top level: BBa_K801041
start: 1
end: 57
orientation: inline

BBa_K801036
type: SequenceAnnotation
persistent identity: annotation2199718
display id: annotation2199718
version: 1
top level: BBa_K801041
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801041/annotation2199718/range2199718/1

https://synbiohub.org/public/igem/BBa_K801041/annotation2199718/range2199718/1
type: Range
persistent identity: range2199718
display id: range2199718
version: 1
direction: direction/forward
top level: BBa_K801041
start: 2791
end: 3459
orientation: inline

Essential Gly for photoconvertible binding of PIF3
type: SequenceAnnotation
persistent identity: annotation2199711
display id: annotation2199711
version: 1
top level: BBa_K801041
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K801041/annotation2199711/range2199711/1

https://synbiohub.org/public/igem/BBa_K801041/annotation2199711/range2199711/1
type: Range
persistent identity: range2199711
display id: range2199711
version: 1
direction: direction/forward
top level: BBa_K801041
start: 1102
end: 1104
orientation: inline

Essential Cys for chromophore attachement of phycocyanobilin (PCB)
type: SequenceAnnotation
persistent identity: annotation2199713
display id: annotation2199713
version: 1
top level: BBa_K801041
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K801041/annotation2199713/range2199713/1

https://synbiohub.org/public/igem/BBa_K801041/annotation2199713/range2199713/1
type: Range
persistent identity: range2199713
display id: range2199713
version: 1
direction: direction/forward
top level: BBa_K801041
start: 1129
end: 1131
orientation: inline

RFC25 fusion scar
type: SequenceAnnotation
persistent identity: annotation2199716
display id: annotation2199716
version: 1
top level: BBa_K801041
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801041/annotation2199716/range2199716/1

https://synbiohub.org/public/igem/BBa_K801041/annotation2199716/range2199716/1
type: Range
persistent identity: range2199716
display id: range2199716
version: 1
direction: direction/forward
top level: BBa_K801041
start: 2851
end: 2856
orientation: inline

Essential Arg for photoconvertible binding of PIF3
type: SequenceAnnotation
persistent identity: annotation2199709
display id: annotation2199709
version: 1
top level: BBa_K801041
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K801041/annotation2199709/range2199709/1

https://synbiohub.org/public/igem/BBa_K801041/annotation2199709/range2199709/1
type: Range
persistent identity: range2199709
display id: range2199709
version: 1
direction: direction/forward
top level: BBa_K801041
start: 388
end: 390
orientation: inline

BBa_K801031
type: SequenceAnnotation
persistent identity: annotation2199714
display id: annotation2199714
version: 1
top level: BBa_K801041
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801041/annotation2199714/range2199714/1

https://synbiohub.org/public/igem/BBa_K801041/annotation2199714/range2199714/1
type: Range
persistent identity: range2199714
display id: range2199714
version: 1
direction: direction/forward
top level: BBa_K801041
start: 64
end: 2784
orientation: inline

BBa_K801033
type: SequenceAnnotation
persistent identity: annotation2199717
display id: annotation2199717
version: 1
top level: BBa_K801041
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801041/annotation2199717/range2199717/1

https://synbiohub.org/public/igem/BBa_K801041/annotation2199717/range2199717/1
type: Range
persistent identity: range2199717
display id: range2199717
version: 1
direction: direction/forward
top level: BBa_K801041
start: 2857
end: 3459
orientation: inline

Essential Gly for photoconvertible binding of PIF3
type: SequenceAnnotation
persistent identity: annotation2199710
display id: annotation2199710
version: 1
top level: BBa_K801041
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K801041/annotation2199710/range2199710/1

https://synbiohub.org/public/igem/BBa_K801041/annotation2199710/range2199710/1
type: Range
persistent identity: range2199710
display id: range2199710
version: 1
direction: direction/forward
top level: BBa_K801041
start: 391
end: 393
orientation: inline

BBa_K365005
type: SequenceAnnotation
persistent identity: annotation2199715
display id: annotation2199715
version: 1
top level: BBa_K801041
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801041/annotation2199715/range2199715/1

https://synbiohub.org/public/igem/BBa_K801041/annotation2199715/range2199715/1
type: Range
persistent identity: range2199715
display id: range2199715
version: 1
direction: direction/forward
top level: BBa_K801041
start: 2791
end: 2851
orientation: inline

RFC25 fusion scar
type: SequenceAnnotation
persistent identity: annotation2199724
display id: annotation2199724
version: 1
top level: BBa_K801041
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801041/annotation2199724/range2199724/1

https://synbiohub.org/public/igem/BBa_K801041/annotation2199724/range2199724/1
type: Range
persistent identity: range2199724
display id: range2199724
version: 1
direction: direction/forward
top level: BBa_K801041
start: 2785
end: 2790
orientation: inline

Essential Arg for photoconvertible binding of PIF3
type: SequenceAnnotation
persistent identity: annotation2199712
display id: annotation2199712
version: 1
top level: BBa_K801041
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K801041/annotation2199712/range2199712/1

https://synbiohub.org/public/igem/BBa_K801041/annotation2199712/range2199712/1
type: Range
persistent identity: range2199712
display id: range2199712
version: 1
direction: direction/forward
top level: BBa_K801041
start: 1114
end: 1116
orientation: inline

ZipR34
type: ComponentDefinition
persistent identity: BBa J18924
display id: BBa_J18924
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R34 heterodimerizing leucine zipper
creator: Raik Gruenberg
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Engineered Leu zipper based on VBP B-ZIP domain that forms medium-weak parallel heterodimers with variants E34(X). This zipper corresponds to RR34(A) in Acharya et al and R34 in Bashor et al. Bashor et al. used the three different pairs to tune the affinity of their MAPK feedback device. Interaction with E34(X) variants: * E34(I): Kd = 6.1 nM = J18923 * E34(V): Kd = 41 nM * E34(N): Kd = 810 nM == References == * Acharya et al. (2002) A heterodimerizing leucine zipper coiled coil system for examining the specificity of a position interactions: amino acids I, V, L, N, A, and K. PMID: 12450375 * Bashor et al (2008) Using engineered scaffold interactions to reshape MAP kinase pathway signaling dynamics. PMID: 18339942
mutable notes: * gene synthesis * codon optimized for E. coli
mutable provenance: gene synthesis literature
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_J18924_sequence/1

BBa_J18945
type: ComponentDefinition
persistent identity: BBa J18945
display id: BBa_J18945
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ZipR34 ~ GLucFrag2
creator: Raik Gruenberg
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: P13 expression cassette from Gruenberg et al. 2010 series of interaction reporter proteins. All proteins share the same architecture: * T7Start-domain1-5xGS-domain2-preSCsite-HisTag-T7Stop Only Domains1 and 2 vary and are taken from a list of protein interaction input and output (readout) parts. ===Expression=== Proteins were expressed in E. coli BL21 using T7 Polymerase and were purified by His-tag affinity. ===Reference=== * Gruenberg, Ferrar, van der Sloot, Serrano (2010): Building Blocks for Protein Interaction Devices
mutable notes: Constructs were assembled according to R.F.C. 25. All parts were codon optimized for E. coli.
mutable provenance: synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_J18922
component: BBa_J18913
component: BBa_J18912
component: BBa_B0105
component: BBa_J18929
component: BBa_J18924
component: BBa_J18919
component: BBa_J18909
sequence annotation: BBa_B0105
sequence annotation: BBa_J18929
sequence annotation: BBa_J18919
sequence annotation: BBa_J18912
sequence annotation: BBa_J18924
sequence annotation: BBa_J18909
sequence annotation: BBa_J18922
sequence annotation: BBa_J18913
sequence: https://synbiohub.org/public/igem/BBa_J18945_sequence/1

BBa_J18922
type: Component
persistent identity: component2066653
display id: component2066653
version: 1
top level: BBa_J18945
access: public
definition: https://synbiohub.org/public/igem/BBa_J18922/1

BBa_J18913
type: Component
persistent identity: component2066667
display id: component2066667
version: 1
top level: BBa_J18945
access: public
definition: https://synbiohub.org/public/igem/BBa_J18913/1

BBa_J18912
type: Component
persistent identity: component2066647
display id: component2066647
version: 1
top level: BBa_J18945
access: public
definition: https://synbiohub.org/public/igem/BBa_J18912/1

BBa_B0105
type: Component
persistent identity: component2066658
display id: component2066658
version: 1
top level: BBa_J18945
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_J18929
type: Component
persistent identity: component2066656
display id: component2066656
version: 1
top level: BBa_J18945
access: public
definition: GLucFrag2

BBa_J18924
type: Component
persistent identity: component2066650
display id: component2066650
version: 1
top level: BBa_J18945
access: public
definition: ZipR34

BBa_J18919
type: Component
persistent identity: component2066659
display id: component2066659
version: 1
top level: BBa_J18945
access: public
definition: https://synbiohub.org/public/igem/BBa_J18919/1

BBa_B0105
type: Component
persistent identity: component2066661
display id: component2066661
version: 1
top level: BBa_J18945
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_B0105
type: Component
persistent identity: component2066655
display id: component2066655
version: 1
top level: BBa_J18945
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_B0105
type: Component
persistent identity: component2066649
display id: component2066649
version: 1
top level: BBa_J18945
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_B0105
type: Component
persistent identity: component2066652
display id: component2066652
version: 1
top level: BBa_J18945
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_J18909
type: Component
persistent identity: component2066663
display id: component2066663
version: 1
top level: BBa_J18945
access: public
definition: https://synbiohub.org/public/igem/BBa_J18909/1

BBa_B0105
type: Component
persistent identity: component2066665
display id: component2066665
version: 1
top level: BBa_J18945
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066665
display id: annotation2066665
version: 1
top level: BBa_J18945
location: https://synbiohub.org/public/igem/BBa_J18945/annotation2066665/range2066665/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18945/annotation2066665/range2066665/1
type: Range
persistent identity: range2066665
display id: range2066665
version: 1
direction: direction/reverse
top level: BBa_J18945
start: 543
end: 548
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066661
display id: annotation2066661
version: 1
top level: BBa_J18945
location: https://synbiohub.org/public/igem/BBa_J18945/annotation2066661/range2066661/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18945/annotation2066661/range2066661/1
type: Range
persistent identity: range2066661
display id: range2066661
version: 1
direction: direction/reverse
top level: BBa_J18945
start: 519
end: 524
orientation: inline

BBa_J18929
type: SequenceAnnotation
persistent identity: annotation2066656
display id: annotation2066656
version: 1
top level: BBa_J18945
location: https://synbiohub.org/public/igem/BBa_J18945/annotation2066656/range2066656/1
component: BBa_J18929

https://synbiohub.org/public/igem/BBa_J18945/annotation2066656/range2066656/1
type: Range
persistent identity: range2066656
display id: range2066656
version: 1
direction: direction/reverse
top level: BBa_J18945
start: 261
end: 488
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066652
display id: annotation2066652
version: 1
top level: BBa_J18945
location: https://synbiohub.org/public/igem/BBa_J18945/annotation2066652/range2066652/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18945/annotation2066652/range2066652/1
type: Range
persistent identity: range2066652
display id: range2066652
version: 1
direction: direction/reverse
top level: BBa_J18945
start: 219
end: 224
orientation: inline

BBa_J18919
type: SequenceAnnotation
persistent identity: annotation2066659
display id: annotation2066659
version: 1
top level: BBa_J18945
location: https://synbiohub.org/public/igem/BBa_J18945/annotation2066659/range2066659/1
component: BBa_J18919

https://synbiohub.org/public/igem/BBa_J18945/annotation2066659/range2066659/1
type: Range
persistent identity: range2066659
display id: range2066659
version: 1
direction: direction/reverse
top level: BBa_J18945
start: 495
end: 518
orientation: inline

BBa_J18912
type: SequenceAnnotation
persistent identity: annotation2066647
display id: annotation2066647
version: 1
top level: BBa_J18945
location: https://synbiohub.org/public/igem/BBa_J18945/annotation2066647/range2066647/1
component: BBa_J18912

https://synbiohub.org/public/igem/BBa_J18945/annotation2066647/range2066647/1
type: Range
persistent identity: range2066647
display id: range2066647
version: 1
direction: direction/reverse
top level: BBa_J18945
start: 1
end: 83
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066655
display id: annotation2066655
version: 1
top level: BBa_J18945
location: https://synbiohub.org/public/igem/BBa_J18945/annotation2066655/range2066655/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18945/annotation2066655/range2066655/1
type: Range
persistent identity: range2066655
display id: range2066655
version: 1
direction: direction/reverse
top level: BBa_J18945
start: 255
end: 260
orientation: inline

BBa_J18924
type: SequenceAnnotation
persistent identity: annotation2066650
display id: annotation2066650
version: 1
top level: BBa_J18945
location: https://synbiohub.org/public/igem/BBa_J18945/annotation2066650/range2066650/1
component: BBa_J18924

https://synbiohub.org/public/igem/BBa_J18945/annotation2066650/range2066650/1
type: Range
persistent identity: range2066650
display id: range2066650
version: 1
direction: direction/reverse
top level: BBa_J18945
start: 90
end: 218
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066649
display id: annotation2066649
version: 1
top level: BBa_J18945
location: https://synbiohub.org/public/igem/BBa_J18945/annotation2066649/range2066649/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18945/annotation2066649/range2066649/1
type: Range
persistent identity: range2066649
display id: range2066649
version: 1
direction: direction/reverse
top level: BBa_J18945
start: 84
end: 89
orientation: inline

BBa_J18909
type: SequenceAnnotation
persistent identity: annotation2066663
display id: annotation2066663
version: 1
top level: BBa_J18945
location: https://synbiohub.org/public/igem/BBa_J18945/annotation2066663/range2066663/1
component: BBa_J18909

https://synbiohub.org/public/igem/BBa_J18945/annotation2066663/range2066663/1
type: Range
persistent identity: range2066663
display id: range2066663
version: 1
direction: direction/reverse
top level: BBa_J18945
start: 525
end: 542
orientation: inline

BBa_J18922
type: SequenceAnnotation
persistent identity: annotation2066653
display id: annotation2066653
version: 1
top level: BBa_J18945
location: https://synbiohub.org/public/igem/BBa_J18945/annotation2066653/range2066653/1
component: BBa_J18922

https://synbiohub.org/public/igem/BBa_J18945/annotation2066653/range2066653/1
type: Range
persistent identity: range2066653
display id: range2066653
version: 1
direction: direction/reverse
top level: BBa_J18945
start: 225
end: 254
orientation: inline

BBa_J18913
type: SequenceAnnotation
persistent identity: annotation2066667
display id: annotation2066667
version: 1
top level: BBa_J18945
location: https://synbiohub.org/public/igem/BBa_J18945/annotation2066667/range2066667/1
component: BBa_J18913

https://synbiohub.org/public/igem/BBa_J18945/annotation2066667/range2066667/1
type: Range
persistent identity: range2066667
display id: range2066667
version: 1
direction: direction/reverse
top level: BBa_J18945
start: 549
end: 683
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066658
display id: annotation2066658
version: 1
top level: BBa_J18945
location: https://synbiohub.org/public/igem/BBa_J18945/annotation2066658/range2066658/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18945/annotation2066658/range2066658/1
type: Range
persistent identity: range2066658
display id: range2066658
version: 1
direction: direction/reverse
top level: BBa_J18945
start: 489
end: 494
orientation: inline

BBa_K801042
type: ComponentDefinition
persistent identity: BBa K801042
display id: BBa_K801042
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GAL4 based yeast light-switchable promoter system
creator: Dong-Jiunn Jeffery TRUONG
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1057_
m user id: 0
owner id: 11507
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: composite part of Bba_K319003, BBa_K801039, BBa_K801011 Bba_K319003, Bba_K801040, and BBa_K801011
mutable notes: -
mutable provenance: composite part of Bba_K319003, BBa_K801039, BBa_K801011 Bba_K319003, Bba_K801040, and BBa_K801011
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: HA epitope tage
sequence annotation: T7 promoter (for sequencing)
sequence annotation: GAL4DBD (GAL4 DNA binding domain)
sequence annotation: BBa_K801011, (tTEF1, TEF1 terminator)
sequence annotation: Phytochrome B (PhyB, 908 first N-terminal amino acids)
sequence annotation: BBa_K319003 (pTEF1, TEF1 promoter)
sequence annotation: BBa_K801040 (SV40NLS-PhyB-Linker-GAL4DBD)
sequence annotation: SV40NLS-PhyB-Linker-GAL4DBD
sequence annotation: Linker
sequence annotation: PIF3 (First 100 N-terminal amino acids)
sequence annotation: SV40 NLS (SV40 nuclear localization signal)
sequence annotation: GAL4AD (GAL4 transcriptonal activation domain)
sequence annotation: 20 amino acids long linker (BBa_K365005)
sequence annotation: BBa_K801039 (SV40NLS-GAL4AD-Linker-PIF3)
sequence: https://synbiohub.org/public/igem/BBa_K801042_sequence/1

HA epitope tage
type: SequenceAnnotation
persistent identity: annotation2198787
display id: annotation2198787
version: 1
top level: BBa_K801042
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198787/range2198787/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198787/range2198787/1
type: Range
persistent identity: range2198787
display id: range2198787
version: 1
direction: direction/forward
top level: BBa_K801042
start: 912
end: 932
orientation: inline

T7 promoter (for sequencing)
type: SequenceAnnotation
persistent identity: annotation2198786
display id: annotation2198786
version: 1
top level: BBa_K801042
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198786/range2198786/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198786/range2198786/1
type: Range
persistent identity: range2198786
display id: range2198786
version: 1
direction: direction/forward
top level: BBa_K801042
start: 869
end: 891
orientation: inline

GAL4DBD (GAL4 DNA binding domain)
type: SequenceAnnotation
persistent identity: annotation2198796
display id: annotation2198796
version: 1
top level: BBa_K801042
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198796/range2198796/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198796/range2198796/1
type: Range
persistent identity: range2198796
display id: range2198796
version: 1
direction: direction/forward
top level: BBa_K801042
start: 5102
end: 5539
orientation: inline

BBa_K801011, (tTEF1, TEF1 terminator)
type: SequenceAnnotation
persistent identity: annotation2198816
display id: annotation2198816
version: 1
top level: BBa_K801042
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198816/range2198816/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198816/range2198816/1
type: Range
persistent identity: range2198816
display id: range2198816
version: 1
direction: direction/forward
top level: BBa_K801042
start: 5557
end: 6063
orientation: inline

Phytochrome B (PhyB, 908 first N-terminal amino acids)
type: SequenceAnnotation
persistent identity: annotation2198794
display id: annotation2198794
version: 1
top level: BBa_K801042
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198794/range2198794/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198794/range2198794/1
type: Range
persistent identity: range2198794
display id: range2198794
version: 1
direction: direction/forward
top level: BBa_K801042
start: 2309
end: 5029
orientation: inline

BBa_K319003 (pTEF1, TEF1 promoter)
type: SequenceAnnotation
persistent identity: annotation2198790
display id: annotation2198790
version: 1
top level: BBa_K801042
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198790/range2198790/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198790/range2198790/1
type: Range
persistent identity: range2198790
display id: range2198790
version: 1
direction: direction/forward
top level: BBa_K801042
start: 1782
end: 2231
orientation: inline

BBa_K801040 (SV40NLS-PhyB-Linker-GAL4DBD)
type: SequenceAnnotation
persistent identity: annotation2198793
display id: annotation2198793
version: 1
top level: BBa_K801042
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198793/range2198793/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198793/range2198793/1
type: Range
persistent identity: range2198793
display id: range2198793
version: 1
direction: direction/forward
top level: BBa_K801042
start: 2246
end: 5539
orientation: inline

SV40NLS-PhyB-Linker-GAL4DBD
type: SequenceAnnotation
persistent identity: annotation2198791
display id: annotation2198791
version: 1
top level: BBa_K801042
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198791/range2198791/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198791/range2198791/1
type: Range
persistent identity: range2198791
display id: range2198791
version: 1
direction: direction/forward
top level: BBa_K801042
start: 2237
end: 5548
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2198785
display id: annotation2198785
version: 1
top level: BBa_K801042
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198785/range2198785/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198785/range2198785/1
type: Range
persistent identity: range2198785
display id: range2198785
version: 1
direction: direction/forward
top level: BBa_K801042
start: 864
end: 959
orientation: inline

PIF3 (First 100 N-terminal amino acids)
type: SequenceAnnotation
persistent identity: annotation2198788
display id: annotation2198788
version: 1
top level: BBa_K801042
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198788/range2198788/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198788/range2198788/1
type: Range
persistent identity: range2198788
display id: range2198788
version: 1
direction: direction/forward
top level: BBa_K801042
start: 960
end: 1256
orientation: inline

BBa_K319003 (pTEF1, TEF1 promoter)
type: SequenceAnnotation
persistent identity: annotation2198781
display id: annotation2198781
version: 1
top level: BBa_K801042
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198781/range2198781/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198781/range2198781/1
type: Range
persistent identity: range2198781
display id: range2198781
version: 1
direction: direction/forward
top level: BBa_K801042
start: 1
end: 450
orientation: inline

SV40 NLS (SV40 nuclear localization signal)
type: SequenceAnnotation
persistent identity: annotation2198783
display id: annotation2198783
version: 1
top level: BBa_K801042
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198783/range2198783/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198783/range2198783/1
type: Range
persistent identity: range2198783
display id: range2198783
version: 1
direction: direction/forward
top level: BBa_K801042
start: 465
end: 521
orientation: inline

GAL4AD (GAL4 transcriptonal activation domain)
type: SequenceAnnotation
persistent identity: annotation2198784
display id: annotation2198784
version: 1
top level: BBa_K801042
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198784/range2198784/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198784/range2198784/1
type: Range
persistent identity: range2198784
display id: range2198784
version: 1
direction: direction/forward
top level: BBa_K801042
start: 525
end: 863
orientation: inline

20 amino acids long linker (BBa_K365005)
type: SequenceAnnotation
persistent identity: annotation2198795
display id: annotation2198795
version: 1
top level: BBa_K801042
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198795/range2198795/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198795/range2198795/1
type: Range
persistent identity: range2198795
display id: range2198795
version: 1
direction: direction/forward
top level: BBa_K801042
start: 5036
end: 5095
orientation: inline

SV40 NLS (SV40 nuclear localization signal)
type: SequenceAnnotation
persistent identity: annotation2198792
display id: annotation2198792
version: 1
top level: BBa_K801042
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198792/range2198792/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198792/range2198792/1
type: Range
persistent identity: range2198792
display id: range2198792
version: 1
direction: direction/forward
top level: BBa_K801042
start: 2246
end: 2302
orientation: inline

BBa_K801039 (SV40NLS-GAL4AD-Linker-PIF3)
type: SequenceAnnotation
persistent identity: annotation2198782
display id: annotation2198782
version: 1
top level: BBa_K801042
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198782/range2198782/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198782/range2198782/1
type: Range
persistent identity: range2198782
display id: range2198782
version: 1
direction: direction/forward
top level: BBa_K801042
start: 456
end: 1259
orientation: inline

BBa_K801011, (tTEF1, TEF1 terminator)
type: SequenceAnnotation
persistent identity: annotation2198789
display id: annotation2198789
version: 1
top level: BBa_K801042
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801042/annotation2198789/range2198789/1

https://synbiohub.org/public/igem/BBa_K801042/annotation2198789/range2198789/1
type: Range
persistent identity: range2198789
display id: range2198789
version: 1
direction: direction/forward
top level: BBa_K801042
start: 1268
end: 1774
orientation: inline

CBDcex
type: ComponentDefinition
persistent identity: BBa K863101
display id: BBa_K863101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cellulose binding Domain of Cellulomonas Fimi Exoglucanse (Freiburg-Standard)
creator: Moritz M??ller
created: 2012-09-17T11:00:00Z
modified: 2015-06-17T01:18:43Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1123_
m user id: 4206
owner id: 13080
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Cellulose binding domain of the Cellulomonas fimi ATCC 484 exoglucanase gene
mutable notes: The sequence starts with the Freiburg-prefix (ATG+NgoMIV-restrictionsite) followed by 12 Bases (4 AS) of the exoglucanase gene in front of the CBD. Then 9 bases of the exoglucanase gene behind the CBD. After that the sequence continues with a short GS-Linker.
mutable provenance: Cloning-Vector with a CBDcex-Barnase fusion-protein
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: Exoglucanase
sequence annotation: NgoMIV
sequence annotation: GS-Linker
sequence annotation: TAA
sequence annotation: AgeI
sequence annotation: Cellulose binding domain (CBDcex)
sequence annotation: ATG
sequence: https://synbiohub.org/public/igem/BBa_K863101_sequence/1

Exoglucanase
type: SequenceAnnotation
persistent identity: annotation2184937
display id: annotation2184937
version: 1
top level: CBDcex
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863101/annotation2184937/range2184937/1

https://synbiohub.org/public/igem/BBa_K863101/annotation2184937/range2184937/1
type: Range
persistent identity: range2184937
display id: range2184937
version: 1
direction: direction/forward
top level: CBDcex
start: 322
end: 330
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2184939
display id: annotation2184939
version: 1
top level: CBDcex
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863101/annotation2184939/range2184939/1

https://synbiohub.org/public/igem/BBa_K863101/annotation2184939/range2184939/1
type: Range
persistent identity: range2184939
display id: range2184939
version: 1
direction: direction/forward
top level: CBDcex
start: 4
end: 9
orientation: inline

GS-Linker
type: SequenceAnnotation
persistent identity: annotation2184938
display id: annotation2184938
version: 1
top level: CBDcex
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863101/annotation2184938/range2184938/1

https://synbiohub.org/public/igem/BBa_K863101/annotation2184938/range2184938/1
type: Range
persistent identity: range2184938
display id: range2184938
version: 1
direction: direction/forward
top level: CBDcex
start: 331
end: 336
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2184934
display id: annotation2184934
version: 1
top level: CBDcex
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863101/annotation2184934/range2184934/1

https://synbiohub.org/public/igem/BBa_K863101/annotation2184934/range2184934/1
type: Range
persistent identity: range2184934
display id: range2184934
version: 1
direction: direction/forward
top level: CBDcex
start: 343
end: 345
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2184940
display id: annotation2184940
version: 1
top level: CBDcex
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863101/annotation2184940/range2184940/1

https://synbiohub.org/public/igem/BBa_K863101/annotation2184940/range2184940/1
type: Range
persistent identity: range2184940
display id: range2184940
version: 1
direction: direction/forward
top level: CBDcex
start: 337
end: 342
orientation: inline

Cellulose binding domain (CBDcex)
type: SequenceAnnotation
persistent identity: annotation2184935
display id: annotation2184935
version: 1
top level: CBDcex
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K863101/annotation2184935/range2184935/1

https://synbiohub.org/public/igem/BBa_K863101/annotation2184935/range2184935/1
type: Range
persistent identity: range2184935
display id: range2184935
version: 1
direction: direction/forward
top level: CBDcex
start: 22
end: 321
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2184933
display id: annotation2184933
version: 1
top level: CBDcex
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863101/annotation2184933/range2184933/1

https://synbiohub.org/public/igem/BBa_K863101/annotation2184933/range2184933/1
type: Range
persistent identity: range2184933
display id: range2184933
version: 1
direction: direction/forward
top level: CBDcex
start: 1
end: 3
orientation: inline

Exoglucanase
type: SequenceAnnotation
persistent identity: annotation2184936
display id: annotation2184936
version: 1
top level: CBDcex
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863101/annotation2184936/range2184936/1

https://synbiohub.org/public/igem/BBa_K863101/annotation2184936/range2184936/1
type: Range
persistent identity: range2184936
display id: range2184936
version: 1
direction: direction/forward
top level: CBDcex
start: 10
end: 21
orientation: inline

CBDclos(J6
type: ComponentDefinition
persistent identity: BBa K863114
display id: BBa_K863114
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CBD of C. cellulovorans cellulose binding protein gene with const. Promoter (J23100+J61101) and GFP
creator: Moritz M??ller
created: 2012-09-22T11:00:00Z
modified: 2015-06-17T01:20:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1123_
m user id: 4206
owner id: 13080
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Cellulose binding Domain of Clostridium cellulovorans [http://www.ncbi.nlm.nih.gov/nuccore/M73817 cellulose binding protein gene (cbp A)] with a constitutive promoter and RBS ( J61101 ) and a fused-GFP protein with His-tag.
mutable notes: Was made to switch from a T7-promoter to a constitutive promoter
mutable provenance: Cellulose binding domain form a cloning-vector. J61101 and [http://partsregistry.org/Part:BBa_I13522 GFP] from the partsregistry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Freiburg-Scar
sequence annotation: NgoMIV
sequence annotation: Cellulose Binding Protein
sequence annotation: TAA
sequence annotation: His-Tag
sequence annotation: BBa_E0040
sequence annotation: ATG
sequence annotation: Cellulose binding domain (CBDclos)
sequence annotation: BBa_K863111
sequence annotation: GS-Linker
sequence annotation: AgeI
sequence annotation: BBa_K863121
sequence annotation: BBa_J23100
sequence annotation: BBa_J61101
sequence: https://synbiohub.org/public/igem/BBa_K863114_sequence/1

Freiburg-Scar
type: SequenceAnnotation
persistent identity: annotation2194138
display id: annotation2194138
version: 1
top level: CBDclos(J6
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194138/range2194138/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194138/range2194138/1
type: Range
persistent identity: range2194138
display id: range2194138
version: 1
direction: direction/forward
top level: CBDclos(J6
start: 371
end: 376
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2194133
display id: annotation2194133
version: 1
top level: CBDclos(J6
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194133/range2194133/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194133/range2194133/1
type: Range
persistent identity: range2194133
display id: range2194133
version: 1
direction: direction/forward
top level: CBDclos(J6
start: 65
end: 70
orientation: inline

Cellulose Binding Protein
type: SequenceAnnotation
persistent identity: annotation2194136
display id: annotation2194136
version: 1
top level: CBDclos(J6
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194136/range2194136/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194136/range2194136/1
type: Range
persistent identity: range2194136
display id: range2194136
version: 1
direction: direction/forward
top level: CBDclos(J6
start: 359
end: 364
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2194143
display id: annotation2194143
version: 1
top level: CBDclos(J6
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194143/range2194143/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194143/range2194143/1
type: Range
persistent identity: range2194143
display id: range2194143
version: 1
direction: direction/forward
top level: CBDclos(J6
start: 1115
end: 1117
orientation: inline

His-Tag
type: SequenceAnnotation
persistent identity: annotation2194141
display id: annotation2194141
version: 1
top level: CBDclos(J6
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194141/range2194141/1

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type: Range
persistent identity: range2194141
display id: range2194141
version: 1
direction: direction/forward
top level: CBDclos(J6
start: 1091
end: 1108
orientation: inline

Cellulose Binding Protein
type: SequenceAnnotation
persistent identity: annotation2194134
display id: annotation2194134
version: 1
top level: CBDclos(J6
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194134/range2194134/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194134/range2194134/1
type: Range
persistent identity: range2194134
display id: range2194134
version: 1
direction: direction/forward
top level: CBDclos(J6
start: 71
end: 82
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2194140
display id: annotation2194140
version: 1
top level: CBDclos(J6
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194140/range2194140/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194140/range2194140/1
type: Range
persistent identity: range2194140
display id: range2194140
version: 1
direction: direction/forward
top level: CBDclos(J6
start: 377
end: 1090
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2194132
display id: annotation2194132
version: 1
top level: CBDclos(J6
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194132/range2194132/1

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type: Range
persistent identity: range2194132
display id: range2194132
version: 1
direction: direction/forward
top level: CBDclos(J6
start: 62
end: 64
orientation: inline

Cellulose binding domain (CBDclos)
type: SequenceAnnotation
persistent identity: annotation2194135
display id: annotation2194135
version: 1
top level: CBDclos(J6
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194135/range2194135/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194135/range2194135/1
type: Range
persistent identity: range2194135
display id: range2194135
version: 1
direction: direction/forward
top level: CBDclos(J6
start: 83
end: 358
orientation: inline

BBa_K863111
type: SequenceAnnotation
persistent identity: annotation2194131
display id: annotation2194131
version: 1
top level: CBDclos(J6
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194131/range2194131/1

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type: Range
persistent identity: range2194131
display id: range2194131
version: 1
direction: direction/reverse
top level: CBDclos(J6
start: 62
end: 370
orientation: inline

GS-Linker
type: SequenceAnnotation
persistent identity: annotation2194137
display id: annotation2194137
version: 1
top level: CBDclos(J6
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194137/range2194137/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194137/range2194137/1
type: Range
persistent identity: range2194137
display id: range2194137
version: 1
direction: direction/forward
top level: CBDclos(J6
start: 365
end: 370
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2194142
display id: annotation2194142
version: 1
top level: CBDclos(J6
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194142/range2194142/1

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type: Range
persistent identity: range2194142
display id: range2194142
version: 1
direction: direction/forward
top level: CBDclos(J6
start: 1109
end: 1114
orientation: inline

BBa_K863121
type: SequenceAnnotation
persistent identity: annotation2194139
display id: annotation2194139
version: 1
top level: CBDclos(J6
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194139/range2194139/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194139/range2194139/1
type: Range
persistent identity: range2194139
display id: range2194139
version: 1
direction: direction/reverse
top level: CBDclos(J6
start: 377
end: 1117
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2194129
display id: annotation2194129
version: 1
top level: CBDclos(J6
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194129/range2194129/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194129/range2194129/1
type: Range
persistent identity: range2194129
display id: range2194129
version: 1
direction: direction/reverse
top level: CBDclos(J6
start: 1
end: 35
orientation: inline

BBa_J61101
type: SequenceAnnotation
persistent identity: annotation2194130
display id: annotation2194130
version: 1
top level: CBDclos(J6
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194130/range2194130/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194130/range2194130/1
type: Range
persistent identity: range2194130
display id: range2194130
version: 1
direction: direction/reverse
top level: CBDclos(J6
start: 44
end: 55
orientation: inline

BBa_J18943
type: ComponentDefinition
persistent identity: BBa J18943
display id: BBa_J18943
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ZipE34 ~ GLucFrag1
creator: Raik Gruenberg
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
discontinued: false
dominant: false
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: P11 expression cassette from Gruenberg et al. 2010 series of interaction reporter proteins. All proteins share the same architecture: * T7Start-domain1-5xGS-domain2-preSCsite-HisTag-T7Stop Only Domains1 and 2 vary and are taken from a list of protein interaction input and output (readout) parts. ===Expression=== Proteins were expressed in E. coli BL21 using T7 Polymerase and were purified by His-tag affinity. ===Reference=== * Gruenberg, Ferrar, van der Sloot, Serrano (2010): Building Blocks for Protein Interaction Devices
mutable notes: Constructs were assembled according to R.F.C. 25. All parts were codon optimized for E. coli.
mutable provenance: synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_J18919
component: BBa_J18923
component: BBa_B0105
component: BBa_J18922
component: BBa_J18909
component: BBa_J18928
component: BBa_J18913
component: BBa_J18912
sequence annotation: BBa_J18922
sequence annotation: BBa_J18909
sequence annotation: BBa_J18923
sequence annotation: BBa_J18919
sequence annotation: BBa_B0105
sequence annotation: BBa_J18928
sequence annotation: BBa_J18913
sequence annotation: BBa_J18912
sequence: https://synbiohub.org/public/igem/BBa_J18943_sequence/1

BBa_J18919
type: Component
persistent identity: component2066615
display id: component2066615
version: 1
top level: BBa_J18943
access: public
definition: https://synbiohub.org/public/igem/BBa_J18919/1

BBa_J18923
type: Component
persistent identity: component2066606
display id: component2066606
version: 1
top level: BBa_J18943
access: public
definition: ZipE34

BBa_B0105
type: Component
persistent identity: component2066611
display id: component2066611
version: 1
top level: BBa_J18943
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_J18922
type: Component
persistent identity: component2066609
display id: component2066609
version: 1
top level: BBa_J18943
access: public
definition: https://synbiohub.org/public/igem/BBa_J18922/1

BBa_B0105
type: Component
persistent identity: component2066614
display id: component2066614
version: 1
top level: BBa_J18943
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_J18909
type: Component
persistent identity: component2066619
display id: component2066619
version: 1
top level: BBa_J18943
access: public
definition: https://synbiohub.org/public/igem/BBa_J18909/1

BBa_B0105
type: Component
persistent identity: component2066621
display id: component2066621
version: 1
top level: BBa_J18943
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_B0105
type: Component
persistent identity: component2066617
display id: component2066617
version: 1
top level: BBa_J18943
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_J18928
type: Component
persistent identity: component2066612
display id: component2066612
version: 1
top level: BBa_J18943
access: public
definition: GLucFrag1

BBa_J18913
type: Component
persistent identity: component2066623
display id: component2066623
version: 1
top level: BBa_J18943
access: public
definition: https://synbiohub.org/public/igem/BBa_J18913/1

BBa_J18912
type: Component
persistent identity: component2066603
display id: component2066603
version: 1
top level: BBa_J18943
access: public
definition: https://synbiohub.org/public/igem/BBa_J18912/1

BBa_B0105
type: Component
persistent identity: component2066605
display id: component2066605
version: 1
top level: BBa_J18943
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_B0105
type: Component
persistent identity: component2066608
display id: component2066608
version: 1
top level: BBa_J18943
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_J18922
type: SequenceAnnotation
persistent identity: annotation2066609
display id: annotation2066609
version: 1
top level: BBa_J18943
location: https://synbiohub.org/public/igem/BBa_J18943/annotation2066609/range2066609/1
component: BBa_J18922

https://synbiohub.org/public/igem/BBa_J18943/annotation2066609/range2066609/1
type: Range
persistent identity: range2066609
display id: range2066609
version: 1
direction: direction/reverse
top level: BBa_J18943
start: 225
end: 254
orientation: inline

BBa_J18909
type: SequenceAnnotation
persistent identity: annotation2066619
display id: annotation2066619
version: 1
top level: BBa_J18943
location: https://synbiohub.org/public/igem/BBa_J18943/annotation2066619/range2066619/1
component: BBa_J18909

https://synbiohub.org/public/igem/BBa_J18943/annotation2066619/range2066619/1
type: Range
persistent identity: range2066619
display id: range2066619
version: 1
direction: direction/reverse
top level: BBa_J18943
start: 573
end: 590
orientation: inline

BBa_J18923
type: SequenceAnnotation
persistent identity: annotation2066606
display id: annotation2066606
version: 1
top level: BBa_J18943
location: https://synbiohub.org/public/igem/BBa_J18943/annotation2066606/range2066606/1
component: BBa_J18923

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type: Range
persistent identity: range2066606
display id: range2066606
version: 1
direction: direction/reverse
top level: BBa_J18943
start: 90
end: 218
orientation: inline

BBa_J18919
type: SequenceAnnotation
persistent identity: annotation2066615
display id: annotation2066615
version: 1
top level: BBa_J18943
location: https://synbiohub.org/public/igem/BBa_J18943/annotation2066615/range2066615/1
component: BBa_J18919

https://synbiohub.org/public/igem/BBa_J18943/annotation2066615/range2066615/1
type: Range
persistent identity: range2066615
display id: range2066615
version: 1
direction: direction/reverse
top level: BBa_J18943
start: 543
end: 566
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066608
display id: annotation2066608
version: 1
top level: BBa_J18943
location: https://synbiohub.org/public/igem/BBa_J18943/annotation2066608/range2066608/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18943/annotation2066608/range2066608/1
type: Range
persistent identity: range2066608
display id: range2066608
version: 1
direction: direction/reverse
top level: BBa_J18943
start: 219
end: 224
orientation: inline

BBa_J18928
type: SequenceAnnotation
persistent identity: annotation2066612
display id: annotation2066612
version: 1
top level: BBa_J18943
location: https://synbiohub.org/public/igem/BBa_J18943/annotation2066612/range2066612/1
component: BBa_J18928

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type: Range
persistent identity: range2066612
display id: range2066612
version: 1
direction: direction/reverse
top level: BBa_J18943
start: 261
end: 536
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066617
display id: annotation2066617
version: 1
top level: BBa_J18943
location: https://synbiohub.org/public/igem/BBa_J18943/annotation2066617/range2066617/1
component: BBa_B0105

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type: Range
persistent identity: range2066617
display id: range2066617
version: 1
direction: direction/reverse
top level: BBa_J18943
start: 567
end: 572
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066605
display id: annotation2066605
version: 1
top level: BBa_J18943
location: https://synbiohub.org/public/igem/BBa_J18943/annotation2066605/range2066605/1
component: BBa_B0105

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type: Range
persistent identity: range2066605
display id: range2066605
version: 1
direction: direction/reverse
top level: BBa_J18943
start: 84
end: 89
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066614
display id: annotation2066614
version: 1
top level: BBa_J18943
location: https://synbiohub.org/public/igem/BBa_J18943/annotation2066614/range2066614/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18943/annotation2066614/range2066614/1
type: Range
persistent identity: range2066614
display id: range2066614
version: 1
direction: direction/reverse
top level: BBa_J18943
start: 537
end: 542
orientation: inline

BBa_J18913
type: SequenceAnnotation
persistent identity: annotation2066623
display id: annotation2066623
version: 1
top level: BBa_J18943
location: https://synbiohub.org/public/igem/BBa_J18943/annotation2066623/range2066623/1
component: BBa_J18913

https://synbiohub.org/public/igem/BBa_J18943/annotation2066623/range2066623/1
type: Range
persistent identity: range2066623
display id: range2066623
version: 1
direction: direction/reverse
top level: BBa_J18943
start: 597
end: 731
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066611
display id: annotation2066611
version: 1
top level: BBa_J18943
location: https://synbiohub.org/public/igem/BBa_J18943/annotation2066611/range2066611/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18943/annotation2066611/range2066611/1
type: Range
persistent identity: range2066611
display id: range2066611
version: 1
direction: direction/reverse
top level: BBa_J18943
start: 255
end: 260
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066621
display id: annotation2066621
version: 1
top level: BBa_J18943
location: https://synbiohub.org/public/igem/BBa_J18943/annotation2066621/range2066621/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18943/annotation2066621/range2066621/1
type: Range
persistent identity: range2066621
display id: range2066621
version: 1
direction: direction/reverse
top level: BBa_J18943
start: 591
end: 596
orientation: inline

BBa_J18912
type: SequenceAnnotation
persistent identity: annotation2066603
display id: annotation2066603
version: 1
top level: BBa_J18943
location: https://synbiohub.org/public/igem/BBa_J18943/annotation2066603/range2066603/1
component: BBa_J18912

https://synbiohub.org/public/igem/BBa_J18943/annotation2066603/range2066603/1
type: Range
persistent identity: range2066603
display id: range2066603
version: 1
direction: direction/reverse
top level: BBa_J18943
start: 1
end: 83
orientation: inline

BBa_K772100
type: ComponentDefinition
persistent identity: BBa K772100
display id: BBa_K772100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: EpCAM Detection Device
creator: Mustafa Elitok
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:15Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1024_
m user id: 0
owner id: 9573
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This gene sequence includes both of EpCAM Binding Docks; BBa_K772001 and BBa_K772002. Thus, this composite part is the main component of CTC (Circulating Tumor Cell) detection system. Docks include an EpCAM (Epithelial Cell Adhesion Molecule) antigen binding domain, OmpA transmembrane protein (dock) and TEV protease C or N terminus. Hopefully, after binding to the EpCAM antigen, TEV protease domains will fix together to form the full structure in order to activate the signalling pathway. (For more information: BBa_K772003) EpCAM is an natural antigen which is found high significantly in the cases of some breast and colorectal cancer subtypes. On the upstream region of protein sequences, there are a constitutive pLac promoter and appropriate RBS. To see how this part works: Experience.
mutable notes: There are two protein sequences between domains and the dock: One between the binding domain (antibody) and dock and one between the domain of TEV protease.
mutable provenance: CD225 (EpCAM antibody) is commonly used for the test of presence of EpCAM in the laboratories. The sequences of OmpA and TEV protease terminuses are acquired from the Registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_K772001
component: BBa_K772002
sequence annotation: BBa_K772002
sequence annotation: BBa_K772001
sequence: https://synbiohub.org/public/igem/BBa_K772100_sequence/1

BBa_K772001
type: Component
persistent identity: component2211341
display id: component2211341
version: 1
top level: BBa_K772100
access: public
definition: BBa_K772001

BBa_K772002
type: Component
persistent identity: component2211348
display id: component2211348
version: 1
top level: BBa_K772100
access: public
definition: BBa_K772002

BBa_K772002
type: SequenceAnnotation
persistent identity: annotation2211348
display id: annotation2211348
version: 1
top level: BBa_K772100
location: https://synbiohub.org/public/igem/BBa_K772100/annotation2211348/range2211348/1
component: BBa_K772002

https://synbiohub.org/public/igem/BBa_K772100/annotation2211348/range2211348/1
type: Range
persistent identity: range2211348
display id: range2211348
version: 1
direction: direction/reverse
top level: BBa_K772100
start: 1168
end: 2305
orientation: inline

BBa_K772001
type: SequenceAnnotation
persistent identity: annotation2211341
display id: annotation2211341
version: 1
top level: BBa_K772100
location: https://synbiohub.org/public/igem/BBa_K772100/annotation2211341/range2211341/1
component: BBa_K772001

https://synbiohub.org/public/igem/BBa_K772100/annotation2211341/range2211341/1
type: Range
persistent identity: range2211341
display id: range2211341
version: 1
direction: direction/reverse
top level: BBa_K772100
start: 1
end: 1159
orientation: inline

BBa_J18947
type: ComponentDefinition
persistent identity: BBa J18947
display id: BBa_J18947
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lov2 ~ GLucFrag1
creator: Raik Gruenberg
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
discontinued: false
dominant: false
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: P15 expression cassette from Gruenberg et al. 2010 series of interaction reporter proteins. All proteins share the same architecture: * T7Start-domain1-5xGS-domain2-preSCsite-HisTag-T7Stop Only Domains1 and 2 vary and are taken from a list of protein interaction input and output (readout) parts. ===Expression=== Proteins were expressed in E. coli BL21 using T7 Polymerase and were purified by His-tag affinity. ===Reference=== * Gruenberg, Ferrar, van der Sloot, Serrano (2010): Building Blocks for Protein Interaction Devices
mutable notes: Constructs were assembled according to R.F.C. 25. All parts were codon optimized for E. coli.
mutable provenance: synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0105
component: BBa_J18919
component: BBa_J18927
component: BBa_J18913
component: BBa_J18909
component: BBa_J18912
component: BBa_J18922
component: BBa_J18928
sequence annotation: BBa_J18928
sequence annotation: BBa_B0105
sequence annotation: BBa_J18919
sequence annotation: BBa_J18927
sequence annotation: BBa_J18922
sequence annotation: BBa_J18912
sequence annotation: BBa_J18913
sequence annotation: BBa_J18909
sequence: https://synbiohub.org/public/igem/BBa_J18947_sequence/1

BBa_B0105
type: Component
persistent identity: component2066699
display id: component2066699
version: 1
top level: BBa_J18947
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_J18919
type: Component
persistent identity: component2066706
display id: component2066706
version: 1
top level: BBa_J18947
access: public
definition: https://synbiohub.org/public/igem/BBa_J18919/1

BBa_J18927
type: Component
persistent identity: component2066697
display id: component2066697
version: 1
top level: BBa_J18947
access: public
definition: Lov2

BBa_B0105
type: Component
persistent identity: component2066712
display id: component2066712
version: 1
top level: BBa_J18947
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_J18913
type: Component
persistent identity: component2066714
display id: component2066714
version: 1
top level: BBa_J18947
access: public
definition: https://synbiohub.org/public/igem/BBa_J18913/1

BBa_J18909
type: Component
persistent identity: component2066710
display id: component2066710
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BBa_J18922
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BBa_J18928
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BBa_B0105
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BBa_B0105
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BBa_B0105
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BBa_B0105
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BBa_J18909
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BBa_J18950
type: ComponentDefinition
persistent identity: BBa J18950
display id: BBa_J18950
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mCitrine ~ Lov2
creator: Raik Gruenberg
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
discontinued: false
dominant: false
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: P18 expression cassette from Gruenberg et al. 2010 series of interaction reporter proteins. All proteins share the same architecture: * T7Start-domain1-5xGS-domain2-preSCsite-HisTag-T7Stop Only Domains1 and 2 vary and are taken from a list of protein interaction input and output (readout) parts. ===Expression=== Proteins were expressed in E. coli BL21 using T7 Polymerase and were purified by His-tag affinity. ===Reference=== * Gruenberg, Ferrar, van der Sloot, Serrano (2010): Building Blocks for Protein Interaction Devices
mutable notes: Constructs were assembled according to R.F.C. 25. All parts were codon optimized for E. coli.
mutable provenance: synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_J18922
component: BBa_J18913
component: BBa_J18912
component: BBa_J18927
component: BBa_B0105
component: BBa_J18909
component: BBa_J18931
component: BBa_J18919
sequence annotation: BBa_J18909
sequence annotation: BBa_J18913
sequence annotation: BBa_B0105
sequence annotation: BBa_J18922
sequence annotation: BBa_J18931
sequence annotation: BBa_J18927
sequence annotation: BBa_J18919
sequence annotation: BBa_J18912
sequence: https://synbiohub.org/public/igem/BBa_J18950_sequence/1

BBa_J18922
type: Component
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definition: https://synbiohub.org/public/igem/BBa_J18922/1

BBa_J18913
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definition: https://synbiohub.org/public/igem/BBa_J18913/1

BBa_J18912
type: Component
persistent identity: component2066760
display id: component2066760
version: 1
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definition: https://synbiohub.org/public/igem/BBa_J18912/1

BBa_J18927
type: Component
persistent identity: component2066769
display id: component2066769
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access: public
definition: Lov2

BBa_B0105
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definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_J18909
type: Component
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definition: https://synbiohub.org/public/igem/BBa_J18909/1

BBa_B0105
type: Component
persistent identity: component2066768
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definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_B0105
type: Component
persistent identity: component2066774
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definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_J18931
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persistent identity: component2066763
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definition: https://synbiohub.org/public/igem/BBa_J18931/1

BBa_B0105
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definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_B0105
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BBa_J18919
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definition: https://synbiohub.org/public/igem/BBa_J18919/1

BBa_B0105
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persistent identity: component2066762
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definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_J18909
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BBa_J18913
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display id: range2066780
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start: 1320
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BBa_B0105
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persistent identity: range2066768
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direction: direction/reverse
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start: 840
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BBa_J18922
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direction: direction/reverse
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start: 810
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BBa_J18931
type: SequenceAnnotation
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persistent identity: range2066763
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direction: direction/reverse
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BBa_B0105
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start: 1290
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BBa_J18927
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direction: direction/reverse
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start: 846
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BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066762
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persistent identity: range2066762
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direction: direction/reverse
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start: 84
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BBa_J18919
type: SequenceAnnotation
persistent identity: annotation2066772
display id: annotation2066772
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persistent identity: range2066772
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start: 1266
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BBa_B0105
type: SequenceAnnotation
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persistent identity: range2066771
display id: range2066771
version: 1
direction: direction/reverse
top level: BBa_J18950
start: 1260
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BBa_J18912
type: SequenceAnnotation
persistent identity: annotation2066760
display id: annotation2066760
version: 1
top level: BBa_J18950
location: https://synbiohub.org/public/igem/BBa_J18950/annotation2066760/range2066760/1
component: BBa_J18912

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type: Range
persistent identity: range2066760
display id: range2066760
version: 1
direction: direction/reverse
top level: BBa_J18950
start: 1
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BBa_K1333202
type: ComponentDefinition
persistent identity: BBa K1333202
display id: BBa_K1333202
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lambda cI-LGF2(R74A)
creator: Yanwen Xie
created: 2014-09-22T11:00:00Z
modified: 2015-05-08T01:09:58Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1708_
m user id: 0
owner id: 15838
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Fuse lambda cI with LGF2 mutated from Arg to Ala at site 74 (the Glu which locates after three continuous Ala behind lambda repressor is at site 58). As one of the components in Bacterial Two-hybrid System, it causes a stronger interaction with GAL11P compared to the normalized WT.
mutable notes: The LGF2 is mutated from CGA to GCA at site 74.
mutable provenance: It is from Agilent Technologies' BacterioMatch ⅡTwo-Hybrid System Vector Kit and modified by Site-Directed Mutagenesis(SDM).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: TGA stop codon
sequence annotation: lambda cI
sequence annotation: ATG start codon
sequence annotation: LGF2(R74A)
sequence: https://synbiohub.org/public/igem/BBa_K1333202_sequence/1

TGA stop codon
type: SequenceAnnotation
persistent identity: annotation2385729
display id: annotation2385729
version: 1
top level: BBa_K1333202
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1333202/annotation2385729/range2385729/1

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type: Range
persistent identity: range2385729
display id: range2385729
version: 1
direction: direction/forward
top level: BBa_K1333202
start: 841
end: 843
orientation: inline

lambda cI
type: SequenceAnnotation
persistent identity: annotation2385730
display id: annotation2385730
version: 1
top level: BBa_K1333202
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1333202/annotation2385730/range2385730/1

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type: Range
persistent identity: range2385730
display id: range2385730
version: 1
direction: direction/forward
top level: BBa_K1333202
start: 1
end: 711
orientation: inline

ATG start codon
type: SequenceAnnotation
persistent identity: annotation2385728
display id: annotation2385728
version: 1
top level: BBa_K1333202
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1333202/annotation2385728/range2385728/1

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type: Range
persistent identity: range2385728
display id: range2385728
version: 1
direction: direction/forward
top level: BBa_K1333202
start: 1
end: 3
orientation: inline

LGF2(R74A)
type: SequenceAnnotation
persistent identity: annotation2385731
display id: annotation2385731
version: 1
top level: BBa_K1333202
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1333202/annotation2385731/range2385731/1

https://synbiohub.org/public/igem/BBa_K1333202/annotation2385731/range2385731/1
type: Range
persistent identity: range2385731
display id: range2385731
version: 1
direction: direction/forward
top level: BBa_K1333202
start: 721
end: 840
orientation: inline

BBa_J18954
type: ComponentDefinition
persistent identity: BBa J18954
display id: BBa_J18954
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ZipR34 ~ mCitrine
creator: Raik Gruenberg
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
discontinued: false
dominant: false
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: P22 expression cassette from Gruenberg et al. 2010 series of interaction reporter proteins. All proteins share the same architecture: * T7Start-domain1-5xGS-domain2-preSCsite-HisTag-T7Stop Only Domains1 and 2 vary and are taken from a list of protein interaction input and output (readout) parts. ===Expression=== Proteins were expressed in E. coli BL21 using T7 Polymerase and were purified by His-tag affinity. ===Reference=== * Gruenberg, Ferrar, van der Sloot, Serrano (2010): Building Blocks for Protein Interaction Devices
mutable notes: Constructs were assembled according to R.F.C. 25. All parts were codon optimized for E. coli.
mutable provenance: synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0105
component: BBa_J18931
component: BBa_J18909
component: BBa_J18913
component: BBa_J18922
component: BBa_J18919
component: BBa_J18924
component: BBa_J18912
sequence annotation: BBa_B0105
sequence annotation: BBa_J18913
sequence annotation: BBa_J18931
sequence annotation: BBa_J18919
sequence annotation: BBa_J18922
sequence annotation: BBa_J18912
sequence annotation: BBa_J18924
sequence annotation: BBa_J18909
sequence: https://synbiohub.org/public/igem/BBa_J18954_sequence/1

BBa_B0105
type: Component
persistent identity: component2066994
display id: component2066994
version: 1
top level: BBa_J18954
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_J18931
type: Component
persistent identity: component2066989
display id: component2066989
version: 1
top level: BBa_J18954
access: public
definition: https://synbiohub.org/public/igem/BBa_J18931/1

BBa_B0105
type: Component
persistent identity: component2066991
display id: component2066991
version: 1
top level: BBa_J18954
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_B0105
type: Component
persistent identity: component2066998
display id: component2066998
version: 1
top level: BBa_J18954
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_J18909
type: Component
persistent identity: component2066996
display id: component2066996
version: 1
top level: BBa_J18954
access: public
definition: https://synbiohub.org/public/igem/BBa_J18909/1

BBa_J18913
type: Component
persistent identity: component2067000
display id: component2067000
version: 1
top level: BBa_J18954
access: public
definition: https://synbiohub.org/public/igem/BBa_J18913/1

BBa_J18922
type: Component
persistent identity: component2066986
display id: component2066986
version: 1
top level: BBa_J18954
access: public
definition: https://synbiohub.org/public/igem/BBa_J18922/1

BBa_J18919
type: Component
persistent identity: component2066992
display id: component2066992
version: 1
top level: BBa_J18954
access: public
definition: https://synbiohub.org/public/igem/BBa_J18919/1

BBa_B0105
type: Component
persistent identity: component2066982
display id: component2066982
version: 1
top level: BBa_J18954
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_B0105
type: Component
persistent identity: component2066985
display id: component2066985
version: 1
top level: BBa_J18954
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_J18924
type: Component
persistent identity: component2066983
display id: component2066983
version: 1
top level: BBa_J18954
access: public
definition: ZipR34

BBa_J18912
type: Component
persistent identity: component2066980
display id: component2066980
version: 1
top level: BBa_J18954
access: public
definition: https://synbiohub.org/public/igem/BBa_J18912/1

BBa_B0105
type: Component
persistent identity: component2066988
display id: component2066988
version: 1
top level: BBa_J18954
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066994
display id: annotation2066994
version: 1
top level: BBa_J18954
location: https://synbiohub.org/public/igem/BBa_J18954/annotation2066994/range2066994/1
component: BBa_B0105

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type: Range
persistent identity: range2066994
display id: range2066994
version: 1
direction: direction/reverse
top level: BBa_J18954
start: 1005
end: 1010
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066998
display id: annotation2066998
version: 1
top level: BBa_J18954
location: https://synbiohub.org/public/igem/BBa_J18954/annotation2066998/range2066998/1
component: BBa_B0105

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type: Range
persistent identity: range2066998
display id: range2066998
version: 1
direction: direction/reverse
top level: BBa_J18954
start: 1029
end: 1034
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066991
display id: annotation2066991
version: 1
top level: BBa_J18954
location: https://synbiohub.org/public/igem/BBa_J18954/annotation2066991/range2066991/1
component: BBa_B0105

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type: Range
persistent identity: range2066991
display id: range2066991
version: 1
direction: direction/reverse
top level: BBa_J18954
start: 975
end: 980
orientation: inline

BBa_J18913
type: SequenceAnnotation
persistent identity: annotation2067000
display id: annotation2067000
version: 1
top level: BBa_J18954
location: https://synbiohub.org/public/igem/BBa_J18954/annotation2067000/range2067000/1
component: BBa_J18913

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type: Range
persistent identity: range2067000
display id: range2067000
version: 1
direction: direction/reverse
top level: BBa_J18954
start: 1035
end: 1169
orientation: inline

BBa_J18931
type: SequenceAnnotation
persistent identity: annotation2066989
display id: annotation2066989
version: 1
top level: BBa_J18954
location: https://synbiohub.org/public/igem/BBa_J18954/annotation2066989/range2066989/1
component: BBa_J18931

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type: Range
persistent identity: range2066989
display id: range2066989
version: 1
direction: direction/reverse
top level: BBa_J18954
start: 261
end: 974
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066988
display id: annotation2066988
version: 1
top level: BBa_J18954
location: https://synbiohub.org/public/igem/BBa_J18954/annotation2066988/range2066988/1
component: BBa_B0105

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type: Range
persistent identity: range2066988
display id: range2066988
version: 1
direction: direction/reverse
top level: BBa_J18954
start: 255
end: 260
orientation: inline

BBa_J18919
type: SequenceAnnotation
persistent identity: annotation2066992
display id: annotation2066992
version: 1
top level: BBa_J18954
location: https://synbiohub.org/public/igem/BBa_J18954/annotation2066992/range2066992/1
component: BBa_J18919

https://synbiohub.org/public/igem/BBa_J18954/annotation2066992/range2066992/1
type: Range
persistent identity: range2066992
display id: range2066992
version: 1
direction: direction/reverse
top level: BBa_J18954
start: 981
end: 1004
orientation: inline

BBa_J18922
type: SequenceAnnotation
persistent identity: annotation2066986
display id: annotation2066986
version: 1
top level: BBa_J18954
location: https://synbiohub.org/public/igem/BBa_J18954/annotation2066986/range2066986/1
component: BBa_J18922

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type: Range
persistent identity: range2066986
display id: range2066986
version: 1
direction: direction/reverse
top level: BBa_J18954
start: 225
end: 254
orientation: inline

BBa_J18912
type: SequenceAnnotation
persistent identity: annotation2066980
display id: annotation2066980
version: 1
top level: BBa_J18954
location: https://synbiohub.org/public/igem/BBa_J18954/annotation2066980/range2066980/1
component: BBa_J18912

https://synbiohub.org/public/igem/BBa_J18954/annotation2066980/range2066980/1
type: Range
persistent identity: range2066980
display id: range2066980
version: 1
direction: direction/reverse
top level: BBa_J18954
start: 1
end: 83
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066982
display id: annotation2066982
version: 1
top level: BBa_J18954
location: https://synbiohub.org/public/igem/BBa_J18954/annotation2066982/range2066982/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18954/annotation2066982/range2066982/1
type: Range
persistent identity: range2066982
display id: range2066982
version: 1
direction: direction/reverse
top level: BBa_J18954
start: 84
end: 89
orientation: inline

BBa_J18924
type: SequenceAnnotation
persistent identity: annotation2066983
display id: annotation2066983
version: 1
top level: BBa_J18954
location: https://synbiohub.org/public/igem/BBa_J18954/annotation2066983/range2066983/1
component: BBa_J18924

https://synbiohub.org/public/igem/BBa_J18954/annotation2066983/range2066983/1
type: Range
persistent identity: range2066983
display id: range2066983
version: 1
direction: direction/reverse
top level: BBa_J18954
start: 90
end: 218
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066985
display id: annotation2066985
version: 1
top level: BBa_J18954
location: https://synbiohub.org/public/igem/BBa_J18954/annotation2066985/range2066985/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18954/annotation2066985/range2066985/1
type: Range
persistent identity: range2066985
display id: range2066985
version: 1
direction: direction/reverse
top level: BBa_J18954
start: 219
end: 224
orientation: inline

BBa_J18909
type: SequenceAnnotation
persistent identity: annotation2066996
display id: annotation2066996
version: 1
top level: BBa_J18954
location: https://synbiohub.org/public/igem/BBa_J18954/annotation2066996/range2066996/1
component: BBa_J18909

https://synbiohub.org/public/igem/BBa_J18954/annotation2066996/range2066996/1
type: Range
persistent identity: range2066996
display id: range2066996
version: 1
direction: direction/reverse
top level: BBa_J18954
start: 1011
end: 1028
orientation: inline

ZipE34
type: ComponentDefinition
persistent identity: BBa J18923
display id: BBa_J18923
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: E34(I) strong heterodimerizing leucine zipper
creator: Raik Gruenberg
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Engineered Leu zipper based on VBP B-ZIP domain that forms strong parallel heterodimers with R34. Interaction with R34: * Kd = 6.1 nM References ============ * Acharya et al. (2002) A heterodimerizing leucine zipper coiled coil system for examining the specificity of a position interactions: amino acids I, V, L, N, A, and K. PMID: 12450375 * Bashor et al (2008) Using engineered scaffold interactions to reshape MAP kinase pathway signaling dynamics. PMID: 18339942
mutable notes: * gene synthesis * codon optimized for E. coli
mutable provenance: gene synthesis literature
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_J18923_sequence/1

BBa_K801040
type: ComponentDefinition
persistent identity: BBa K801040
display id: BBa_K801040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SV40NLS-PhyB-linker-Gal4DBD (part for GAL4 based light switchable promoter system)
creator: Dong-Jiunn Jeffery TRUONG
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1057_
m user id: 0
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Composite part of BBa_K801030, BBa_K801030, BBa_K365005, BBa_K801035
mutable notes: -
mutable provenance: Composite part of BBa_K801030, BBa_K801030, BBa_K365005, BBa_K801035
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_K801031
sequence annotation: BBa_K365005
sequence annotation: Essential Arg for photoconvertible binding of PIF3
sequence annotation: BBa_K801037
sequence annotation: BBa_K801030
sequence annotation: Essential Gly for photoconvertible binding of PIF3
sequence annotation: Essential Cys for chromophore attachement of phycocyanobilin (PCB)
sequence annotation: BBa_K801038
sequence annotation: SV40 nuclear localization sequence (recognized by importin alpha)
sequence annotation: RFC25 fusion scar
sequence annotation: BBa_K801032
sequence: https://synbiohub.org/public/igem/BBa_K801040_sequence/1

BBa_K801031
type: SequenceAnnotation
persistent identity: annotation2199580
display id: annotation2199580
version: 1
top level: BBa_K801040
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801040/annotation2199580/range2199580/1

https://synbiohub.org/public/igem/BBa_K801040/annotation2199580/range2199580/1
type: Range
persistent identity: range2199580
display id: range2199580
version: 1
direction: direction/forward
top level: BBa_K801040
start: 64
end: 2784
orientation: inline

BBa_K365005
type: SequenceAnnotation
persistent identity: annotation2199587
display id: annotation2199587
version: 1
top level: BBa_K801040
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801040/annotation2199587/range2199587/1

https://synbiohub.org/public/igem/BBa_K801040/annotation2199587/range2199587/1
type: Range
persistent identity: range2199587
display id: range2199587
version: 1
direction: direction/forward
top level: BBa_K801040
start: 2791
end: 2850
orientation: inline

Essential Arg for photoconvertible binding of PIF3
type: SequenceAnnotation
persistent identity: annotation2199584
display id: annotation2199584
version: 1
top level: BBa_K801040
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K801040/annotation2199584/range2199584/1

https://synbiohub.org/public/igem/BBa_K801040/annotation2199584/range2199584/1
type: Range
persistent identity: range2199584
display id: range2199584
version: 1
direction: direction/forward
top level: BBa_K801040
start: 1114
end: 1116
orientation: inline

BBa_K801037
type: SequenceAnnotation
persistent identity: annotation2199588
display id: annotation2199588
version: 1
top level: BBa_K801040
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801040/annotation2199588/range2199588/1

https://synbiohub.org/public/igem/BBa_K801040/annotation2199588/range2199588/1
type: Range
persistent identity: range2199588
display id: range2199588
version: 1
direction: direction/forward
top level: BBa_K801040
start: 2791
end: 3294
orientation: inline

BBa_K801030
type: SequenceAnnotation
persistent identity: annotation2199579
display id: annotation2199579
version: 1
top level: BBa_K801040
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801040/annotation2199579/range2199579/1

https://synbiohub.org/public/igem/BBa_K801040/annotation2199579/range2199579/1
type: Range
persistent identity: range2199579
display id: range2199579
version: 1
direction: direction/reverse
top level: BBa_K801040
start: 1
end: 57
orientation: inline

Essential Gly for photoconvertible binding of PIF3
type: SequenceAnnotation
persistent identity: annotation2199583
display id: annotation2199583
version: 1
top level: BBa_K801040
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K801040/annotation2199583/range2199583/1

https://synbiohub.org/public/igem/BBa_K801040/annotation2199583/range2199583/1
type: Range
persistent identity: range2199583
display id: range2199583
version: 1
direction: direction/forward
top level: BBa_K801040
start: 1102
end: 1104
orientation: inline

Essential Cys for chromophore attachement of phycocyanobilin (PCB)
type: SequenceAnnotation
persistent identity: annotation2199585
display id: annotation2199585
version: 1
top level: BBa_K801040
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K801040/annotation2199585/range2199585/1

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type: Range
persistent identity: range2199585
display id: range2199585
version: 1
direction: direction/forward
top level: BBa_K801040
start: 1129
end: 1131
orientation: inline

BBa_K801038
type: SequenceAnnotation
persistent identity: annotation2199591
display id: annotation2199591
version: 1
top level: BBa_K801040
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801040/annotation2199591/range2199591/1

https://synbiohub.org/public/igem/BBa_K801040/annotation2199591/range2199591/1
type: Range
persistent identity: range2199591
display id: range2199591
version: 1
direction: direction/forward
top level: BBa_K801040
start: 64
end: 3294
orientation: inline

SV40 nuclear localization sequence (recognized by importin alpha)
type: SequenceAnnotation
persistent identity: annotation2199578
display id: annotation2199578
version: 1
top level: BBa_K801040
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801040/annotation2199578/range2199578/1

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type: Range
persistent identity: range2199578
display id: range2199578
version: 1
direction: direction/forward
top level: BBa_K801040
start: 22
end: 42
orientation: inline

RFC25 fusion scar
type: SequenceAnnotation
persistent identity: annotation2199586
display id: annotation2199586
version: 1
top level: BBa_K801040
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801040/annotation2199586/range2199586/1

https://synbiohub.org/public/igem/BBa_K801040/annotation2199586/range2199586/1
type: Range
persistent identity: range2199586
display id: range2199586
version: 1
direction: direction/forward
top level: BBa_K801040
start: 2785
end: 2790
orientation: inline

Essential Arg for photoconvertible binding of PIF3
type: SequenceAnnotation
persistent identity: annotation2199581
display id: annotation2199581
version: 1
top level: BBa_K801040
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K801040/annotation2199581/range2199581/1

https://synbiohub.org/public/igem/BBa_K801040/annotation2199581/range2199581/1
type: Range
persistent identity: range2199581
display id: range2199581
version: 1
direction: direction/forward
top level: BBa_K801040
start: 388
end: 390
orientation: inline

BBa_K801032
type: SequenceAnnotation
persistent identity: annotation2199590
display id: annotation2199590
version: 1
top level: BBa_K801040
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801040/annotation2199590/range2199590/1

https://synbiohub.org/public/igem/BBa_K801040/annotation2199590/range2199590/1
type: Range
persistent identity: range2199590
display id: range2199590
version: 1
direction: direction/forward
top level: BBa_K801040
start: 2857
end: 3294
orientation: inline

Essential Gly for photoconvertible binding of PIF3
type: SequenceAnnotation
persistent identity: annotation2199582
display id: annotation2199582
version: 1
top level: BBa_K801040
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K801040/annotation2199582/range2199582/1

https://synbiohub.org/public/igem/BBa_K801040/annotation2199582/range2199582/1
type: Range
persistent identity: range2199582
display id: range2199582
version: 1
direction: direction/forward
top level: BBa_K801040
start: 391
end: 393
orientation: inline

RFC25 fusion scar
type: SequenceAnnotation
persistent identity: annotation2199589
display id: annotation2199589
version: 1
top level: BBa_K801040
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801040/annotation2199589/range2199589/1

https://synbiohub.org/public/igem/BBa_K801040/annotation2199589/range2199589/1
type: Range
persistent identity: range2199589
display id: range2199589
version: 1
direction: direction/forward
top level: BBa_K801040
start: 2851
end: 2856
orientation: inline

BBa_J18942
type: ComponentDefinition
persistent identity: BBa J18942
display id: BBa_J18942
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FRB ~ mCherry
creator: Raik Gruenberg
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
discontinued: false
dominant: false
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: P10 expression cassette from Gruenberg et al. 2010 series of interaction reporter proteins. All proteins share the same architecture: * T7Start-domain1-5xGS-domain2-preSCsite-HisTag-T7Stop Only Domains1 and 2 vary and are taken from a list of protein interaction input and output (readout) parts. ===Expression=== Proteins were expressed in E. coli BL21 using T7 Polymerase and were purified by His-tag affinity. ===Reference=== * Gruenberg, Ferrar, van der Sloot, Serrano (2010): Building Blocks for Protein Interaction Devices
mutable notes: Constructs were assembled according to R.F.C. 25. All parts were codon optimized for E. coli.
mutable provenance: synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
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component: BBa_J18912
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component: BBa_J18922
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sequence annotation: BBa_J18909
sequence annotation: BBa_J18932
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sequence annotation: BBa_J18919
sequence annotation: BBa_J18913
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type: ComponentDefinition
persistent identity: BBa J18955
display id: BBa_J18955
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lov2 ~ mCitrine
creator: Raik Gruenberg
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
discontinued: false
dominant: false
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: P23 expression cassette from Gruenberg et al. 2010 series of interaction reporter proteins. All proteins share the same architecture: * T7Start-domain1-5xGS-domain2-preSCsite-HisTag-T7Stop Only Domains1 and 2 vary and are taken from a list of protein interaction input and output (readout) parts. ===Expression=== Proteins were expressed in E. coli BL21 using T7 Polymerase and were purified by His-tag affinity. ===Reference=== * Gruenberg, Ferrar, van der Sloot, Serrano (2010): Building Blocks for Protein Interaction Devices
mutable notes: Constructs were assembled according to R.F.C. 25. All parts were codon optimized for E. coli.
mutable provenance: synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
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display id: BBa_J18939
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FKBP ~ bla-frag2
creator: Raik Gruenberg
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
discontinued: false
dominant: false
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: P01 expression cassette from Gruenberg et al. 2010 series of interaction reporter proteins. All proteins share the same architecture: * T7Start-domain1-5xGS-domain2-preSCsite-HisTag-T7Stop Only Domains1 and 2 vary and are taken from a list of protein interaction input and output (readout) parts. ===Expression=== Proteins were expressed in E. coli BL21 using T7 Polymerase and were purified by His-tag affinity. ===Reference=== * Gruenberg, Ferrar, van der Sloot, Serrano (2010): Building Blocks for Protein Interaction Devices
mutable notes: Constructs were assembled according to R.F.C. 25. All parts were codon optimized for E. coli.
mutable provenance: synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0105
component: BBa_J18925
component: BBa_J18909
component: BBa_J18919
component: BBa_I757012
component: BBa_J18922
component: BBa_J18912
component: BBa_J18913
sequence annotation: BBa_J18913
sequence annotation: BBa_B0105
sequence annotation: BBa_J18919
sequence annotation: BBa_I757012
sequence annotation: BBa_J18912
sequence annotation: BBa_J18909
sequence annotation: BBa_J18925
sequence annotation: BBa_J18922
sequence: https://synbiohub.org/public/igem/BBa_J18939_sequence/1

BBa_B0105
type: Component
persistent identity: component2066517
display id: component2066517
version: 1
top level: BBa_J18939
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_B0105
type: Component
persistent identity: component2066526
display id: component2066526
version: 1
top level: BBa_J18939
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_J18925
type: Component
persistent identity: component2066515
display id: component2066515
version: 1
top level: BBa_J18939
access: public
definition: FKBP12

BBa_B0105
type: Component
persistent identity: component2066520
display id: component2066520
version: 1
top level: BBa_J18939
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_J18909
type: Component
persistent identity: component2066531
display id: component2066531
version: 1
top level: BBa_J18939
access: public
definition: https://synbiohub.org/public/igem/BBa_J18909/1

BBa_J18919
type: Component
persistent identity: component2066527
display id: component2066527
version: 1
top level: BBa_J18939
access: public
definition: https://synbiohub.org/public/igem/BBa_J18919/1

BBa_B0105
type: Component
persistent identity: component2066514
display id: component2066514
version: 1
top level: BBa_J18939
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_B0105
type: Component
persistent identity: component2066529
display id: component2066529
version: 1
top level: BBa_J18939
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_I757012
type: Component
persistent identity: component2066524
display id: component2066524
version: 1
top level: BBa_J18939
access: public
definition: https://synbiohub.org/public/igem/BBa_I757012/1

BBa_J18922
type: Component
persistent identity: component2066518
display id: component2066518
version: 1
top level: BBa_J18939
access: public
definition: https://synbiohub.org/public/igem/BBa_J18922/1

BBa_J18912
type: Component
persistent identity: component2066512
display id: component2066512
version: 1
top level: BBa_J18939
access: public
definition: https://synbiohub.org/public/igem/BBa_J18912/1

BBa_J18913
type: Component
persistent identity: component2066535
display id: component2066535
version: 1
top level: BBa_J18939
access: public
definition: https://synbiohub.org/public/igem/BBa_J18913/1

BBa_B0105
type: Component
persistent identity: component2066533
display id: component2066533
version: 1
top level: BBa_J18939
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_J18913
type: SequenceAnnotation
persistent identity: annotation2066535
display id: annotation2066535
version: 1
top level: BBa_J18939
location: https://synbiohub.org/public/igem/BBa_J18939/annotation2066535/range2066535/1
component: BBa_J18913

https://synbiohub.org/public/igem/BBa_J18939/annotation2066535/range2066535/1
type: Range
persistent identity: range2066535
display id: range2066535
version: 1
direction: direction/reverse
top level: BBa_J18939
start: 801
end: 935
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066533
display id: annotation2066533
version: 1
top level: BBa_J18939
location: https://synbiohub.org/public/igem/BBa_J18939/annotation2066533/range2066533/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18939/annotation2066533/range2066533/1
type: Range
persistent identity: range2066533
display id: range2066533
version: 1
direction: direction/reverse
top level: BBa_J18939
start: 795
end: 800
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066514
display id: annotation2066514
version: 1
top level: BBa_J18939
location: https://synbiohub.org/public/igem/BBa_J18939/annotation2066514/range2066514/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18939/annotation2066514/range2066514/1
type: Range
persistent identity: range2066514
display id: range2066514
version: 1
direction: direction/reverse
top level: BBa_J18939
start: 84
end: 89
orientation: inline

BBa_J18919
type: SequenceAnnotation
persistent identity: annotation2066527
display id: annotation2066527
version: 1
top level: BBa_J18939
location: https://synbiohub.org/public/igem/BBa_J18939/annotation2066527/range2066527/1
component: BBa_J18919

https://synbiohub.org/public/igem/BBa_J18939/annotation2066527/range2066527/1
type: Range
persistent identity: range2066527
display id: range2066527
version: 1
direction: direction/reverse
top level: BBa_J18939
start: 747
end: 770
orientation: inline

BBa_I757012
type: SequenceAnnotation
persistent identity: annotation2066524
display id: annotation2066524
version: 1
top level: BBa_J18939
location: https://synbiohub.org/public/igem/BBa_J18939/annotation2066524/range2066524/1
component: BBa_I757012

https://synbiohub.org/public/igem/BBa_J18939/annotation2066524/range2066524/1
type: Range
persistent identity: range2066524
display id: range2066524
version: 1
direction: direction/reverse
top level: BBa_J18939
start: 453
end: 740
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066529
display id: annotation2066529
version: 1
top level: BBa_J18939
location: https://synbiohub.org/public/igem/BBa_J18939/annotation2066529/range2066529/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18939/annotation2066529/range2066529/1
type: Range
persistent identity: range2066529
display id: range2066529
version: 1
direction: direction/reverse
top level: BBa_J18939
start: 771
end: 776
orientation: inline

BBa_J18912
type: SequenceAnnotation
persistent identity: annotation2066512
display id: annotation2066512
version: 1
top level: BBa_J18939
location: https://synbiohub.org/public/igem/BBa_J18939/annotation2066512/range2066512/1
component: BBa_J18912

https://synbiohub.org/public/igem/BBa_J18939/annotation2066512/range2066512/1
type: Range
persistent identity: range2066512
display id: range2066512
version: 1
direction: direction/reverse
top level: BBa_J18939
start: 1
end: 83
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066517
display id: annotation2066517
version: 1
top level: BBa_J18939
location: https://synbiohub.org/public/igem/BBa_J18939/annotation2066517/range2066517/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18939/annotation2066517/range2066517/1
type: Range
persistent identity: range2066517
display id: range2066517
version: 1
direction: direction/reverse
top level: BBa_J18939
start: 411
end: 416
orientation: inline

BBa_J18909
type: SequenceAnnotation
persistent identity: annotation2066531
display id: annotation2066531
version: 1
top level: BBa_J18939
location: https://synbiohub.org/public/igem/BBa_J18939/annotation2066531/range2066531/1
component: BBa_J18909

https://synbiohub.org/public/igem/BBa_J18939/annotation2066531/range2066531/1
type: Range
persistent identity: range2066531
display id: range2066531
version: 1
direction: direction/reverse
top level: BBa_J18939
start: 777
end: 794
orientation: inline

BBa_J18925
type: SequenceAnnotation
persistent identity: annotation2066515
display id: annotation2066515
version: 1
top level: BBa_J18939
location: https://synbiohub.org/public/igem/BBa_J18939/annotation2066515/range2066515/1
component: BBa_J18925

https://synbiohub.org/public/igem/BBa_J18939/annotation2066515/range2066515/1
type: Range
persistent identity: range2066515
display id: range2066515
version: 1
direction: direction/reverse
top level: BBa_J18939
start: 90
end: 410
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066526
display id: annotation2066526
version: 1
top level: BBa_J18939
location: https://synbiohub.org/public/igem/BBa_J18939/annotation2066526/range2066526/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18939/annotation2066526/range2066526/1
type: Range
persistent identity: range2066526
display id: range2066526
version: 1
direction: direction/reverse
top level: BBa_J18939
start: 741
end: 746
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066520
display id: annotation2066520
version: 1
top level: BBa_J18939
location: https://synbiohub.org/public/igem/BBa_J18939/annotation2066520/range2066520/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18939/annotation2066520/range2066520/1
type: Range
persistent identity: range2066520
display id: range2066520
version: 1
direction: direction/reverse
top level: BBa_J18939
start: 447
end: 452
orientation: inline

BBa_J18922
type: SequenceAnnotation
persistent identity: annotation2066518
display id: annotation2066518
version: 1
top level: BBa_J18939
location: https://synbiohub.org/public/igem/BBa_J18939/annotation2066518/range2066518/1
component: BBa_J18922

https://synbiohub.org/public/igem/BBa_J18939/annotation2066518/range2066518/1
type: Range
persistent identity: range2066518
display id: range2066518
version: 1
direction: direction/reverse
top level: BBa_J18939
start: 417
end: 446
orientation: inline

BBa_K1478002
type: ComponentDefinition
persistent identity: BBa K1478002
display id: BBa_K1478002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Top metal binding protein. Binds zinc, arsenic, copper and cadmium.
creator: Fabian Fr??mling
created: 2014-09-17T11:00:00Z
modified: 2015-05-27T02:23:01Z
discontinued: false
dominant: false
group u list: _1858_
m user id: 4206
owner id: 20973
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Multiple domain protein. Domains bind zinc, arsenic, copper and cadmium. Sequences derived from metallothioneins and transcription factors of different organisms. Arsenic, zinc and cadmium binding regions are full length metallothioneins. Zinc binding region derived from a transcritpion factor binding domain. Different protein binding regions are separated with glycerin-serine spacers to improve domain folding. Metal binding regions consists of disulfide bonds. For expression in E. coli disulfide bond forming strains are useful (e. g. Origami).
mutable notes: Sequence was optimized for Escherichia coli and Arabidopsis thaliana codon usage.
mutable provenance: Arsenic (CAA06729), zinc (CAD88267) and cadmium (AAN06822) metallothioneins are full length. Copper AAA34542)domain is partial.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1478002_sequence/1

SP-AntiNIP
type: ComponentDefinition
persistent identity: BBa K157031
display id: BBa_K157031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SP-AntiNIP-GGGSlinker-Tm-Splitluciferase1/2
creator: iGEM Team Freiburg 2008
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: true
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Part consists of the signal peptide of Erbb1/EGF-receptor, scFV-antiNIP, GGGSflexlinker (extracellular part), the trans-membrane region of the Erbb1/EGF-receptor and the C-terminal part of the split fluorophor YFP with a fluolinker joined to its N-Terminus (intracellular part).
mutable notes: none
mutable provenance: Derived by cloning of the parts BBa_K157001, BBa_K157003, BBa_K157013, BBa_K157002 and Part 2 of Split- Luciferase For further information see the description of the parts mentioned above.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: SP-AntiNIP-GGGSlinker-Tm-Splitluciferase1/2
sequence annotation: Scar (Thr, Gly)
sequence annotation: AgeI
sequence annotation: GGGS-Linker
sequence annotation: Splitluciferase1/2
sequence annotation: SP
sequence annotation: Tm
sequence annotation: Sc-Fv_anti-NIP
sequence annotation: NgoMI
sequence: https://synbiohub.org/public/igem/BBa_K157031_sequence/1

SP-AntiNIP-GGGSlinker-Tm-Splitluciferase1/2
type: SequenceAnnotation
persistent identity: annotation1990268
display id: annotation1990268
version: 1
top level: SP-AntiNIP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157031/annotation1990268/range1990268/1

https://synbiohub.org/public/igem/BBa_K157031/annotation1990268/range1990268/1
type: Range
persistent identity: range1990268
display id: range1990268
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 1
end: 1236
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993323
display id: annotation1993323
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157031/annotation1993323/range1993323/1

https://synbiohub.org/public/igem/BBa_K157031/annotation1993323/range1993323/1
type: Range
persistent identity: range1993323
display id: range1993323
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 874
end: 879
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation1990267
display id: annotation1990267
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157031/annotation1990267/range1990267/1

https://synbiohub.org/public/igem/BBa_K157031/annotation1990267/range1990267/1
type: Range
persistent identity: range1990267
display id: range1990267
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 1231
end: 1236
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993325
display id: annotation1993325
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157031/annotation1993325/range1993325/1

https://synbiohub.org/public/igem/BBa_K157031/annotation1993325/range1993325/1
type: Range
persistent identity: range1993325
display id: range1993325
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 949
end: 954
orientation: inline

GGGS-Linker
type: SequenceAnnotation
persistent identity: annotation1993322
display id: annotation1993322
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157031/annotation1993322/range1993322/1

https://synbiohub.org/public/igem/BBa_K157031/annotation1993322/range1993322/1
type: Range
persistent identity: range1993322
display id: range1993322
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 829
end: 873
orientation: inline

Splitluciferase1/2
type: SequenceAnnotation
persistent identity: annotation1993326
display id: annotation1993326
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157031/annotation1993326/range1993326/1

https://synbiohub.org/public/igem/BBa_K157031/annotation1993326/range1993326/1
type: Range
persistent identity: range1993326
display id: range1993326
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 955
end: 1230
orientation: inline

SP
type: SequenceAnnotation
persistent identity: annotation1993318
display id: annotation1993318
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157031/annotation1993318/range1993318/1

https://synbiohub.org/public/igem/BBa_K157031/annotation1993318/range1993318/1
type: Range
persistent identity: range1993318
display id: range1993318
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 10
end: 81
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993319
display id: annotation1993319
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157031/annotation1993319/range1993319/1

https://synbiohub.org/public/igem/BBa_K157031/annotation1993319/range1993319/1
type: Range
persistent identity: range1993319
display id: range1993319
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 82
end: 87
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993321
display id: annotation1993321
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157031/annotation1993321/range1993321/1

https://synbiohub.org/public/igem/BBa_K157031/annotation1993321/range1993321/1
type: Range
persistent identity: range1993321
display id: range1993321
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 823
end: 828
orientation: inline

Tm
type: SequenceAnnotation
persistent identity: annotation1993324
display id: annotation1993324
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157031/annotation1993324/range1993324/1

https://synbiohub.org/public/igem/BBa_K157031/annotation1993324/range1993324/1
type: Range
persistent identity: range1993324
display id: range1993324
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 880
end: 948
orientation: inline

Sc-Fv_anti-NIP
type: SequenceAnnotation
persistent identity: annotation1993320
display id: annotation1993320
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157031/annotation1993320/range1993320/1

https://synbiohub.org/public/igem/BBa_K157031/annotation1993320/range1993320/1
type: Range
persistent identity: range1993320
display id: range1993320
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 88
end: 822
orientation: inline

NgoMI
type: SequenceAnnotation
persistent identity: annotation1990266
display id: annotation1990266
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157031/annotation1990266/range1990266/1

https://synbiohub.org/public/igem/BBa_K157031/annotation1990266/range1990266/1
type: Range
persistent identity: range1990266
display id: range1990266
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 4
end: 9
orientation: inline

BBa_K863110
type: ComponentDefinition
persistent identity: BBa K863110
display id: BBa_K863110
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cellulose binding Domain from Clostridium cellulovorans cellulose binding protein
creator: Moritz M??ller
created: 2012-09-19T11:00:00Z
modified: 2015-06-17T01:20:08Z
discontinued: false
dominant: false
group u list: _1123_
m user id: 4206
owner id: 13080
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Cellulose binding Domain from Clostridium cellulovorans [http://www.ncbi.nlm.nih.gov/nuccore/M73817 cellulose binding protein gene] in Freiburg-Standard.
mutable notes: Added Freiburg-pre- and suffix for in frame assembly and a two amino acids (GS)-Linker.
mutable provenance: Cloning-vector
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: NgoMIV
sequence annotation: ATG
sequence annotation: CBP
sequence annotation: Cellulose binding domain (CBDclos)
sequence annotation: TAA
sequence annotation: AgeI
sequence: https://synbiohub.org/public/igem/BBa_K863110_sequence/1

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2188265
display id: annotation2188265
version: 1
top level: BBa_K863110
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863110/annotation2188265/range2188265/1

https://synbiohub.org/public/igem/BBa_K863110/annotation2188265/range2188265/1
type: Range
persistent identity: range2188265
display id: range2188265
version: 1
direction: direction/forward
top level: BBa_K863110
start: 4
end: 9
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2188264
display id: annotation2188264
version: 1
top level: BBa_K863110
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863110/annotation2188264/range2188264/1

https://synbiohub.org/public/igem/BBa_K863110/annotation2188264/range2188264/1
type: Range
persistent identity: range2188264
display id: range2188264
version: 1
direction: direction/forward
top level: BBa_K863110
start: 1
end: 3
orientation: inline

CBP
type: SequenceAnnotation
persistent identity: annotation2188407
display id: annotation2188407
version: 1
top level: BBa_K863110
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863110/annotation2188407/range2188407/1

https://synbiohub.org/public/igem/BBa_K863110/annotation2188407/range2188407/1
type: Range
persistent identity: range2188407
display id: range2188407
version: 1
direction: direction/forward
top level: BBa_K863110
start: 298
end: 303
orientation: inline

Cellulose binding domain (CBDclos)
type: SequenceAnnotation
persistent identity: annotation2188266
display id: annotation2188266
version: 1
top level: BBa_K863110
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K863110/annotation2188266/range2188266/1

https://synbiohub.org/public/igem/BBa_K863110/annotation2188266/range2188266/1
type: Range
persistent identity: range2188266
display id: range2188266
version: 1
direction: direction/forward
top level: BBa_K863110
start: 22
end: 297
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2188268
display id: annotation2188268
version: 1
top level: BBa_K863110
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863110/annotation2188268/range2188268/1

https://synbiohub.org/public/igem/BBa_K863110/annotation2188268/range2188268/1
type: Range
persistent identity: range2188268
display id: range2188268
version: 1
direction: direction/forward
top level: BBa_K863110
start: 310
end: 312
orientation: inline

CBP
type: SequenceAnnotation
persistent identity: annotation2188406
display id: annotation2188406
version: 1
top level: BBa_K863110
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863110/annotation2188406/range2188406/1

https://synbiohub.org/public/igem/BBa_K863110/annotation2188406/range2188406/1
type: Range
persistent identity: range2188406
display id: range2188406
version: 1
direction: direction/forward
top level: BBa_K863110
start: 10
end: 21
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2188267
display id: annotation2188267
version: 1
top level: BBa_K863110
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863110/annotation2188267/range2188267/1

https://synbiohub.org/public/igem/BBa_K863110/annotation2188267/range2188267/1
type: Range
persistent identity: range2188267
display id: range2188267
version: 1
direction: direction/forward
top level: BBa_K863110
start: 304
end: 309
orientation: inline

BBa_K1333200
type: ComponentDefinition
persistent identity: BBa K1333200
display id: BBa_K1333200
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lambda cI-LGF2(E75A)
creator: Yanwen Xie
created: 2014-09-22T11:00:00Z
modified: 2015-05-08T01:09:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1708_
m user id: 0
owner id: 15838
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Fuse lambda cI with LGF2 mutated from Glu to Ala at site 75 (the Glu which locates after three continuous Ala behind lambda repressor is at site 68). As one of the components in Bacterial Two-hybrid System, it causes little interaction with GAL11P compared to the normalized WT.
mutable notes: The LGF2 is mutated from GAA to GCA at site 75.
mutable provenance: It is from Agilent Technologies' BacterioMatch ⅡTwo-Hybrid System Vector Kit and modified by Site-Directed Mutagenesis(SDM).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: LGF2(E75A)
sequence annotation: lambda cI
sequence annotation: ATG start codon
sequence annotation: TGA stop codon
sequence: https://synbiohub.org/public/igem/BBa_K1333200_sequence/1

LGF2(E75A)
type: SequenceAnnotation
persistent identity: annotation2385726
display id: annotation2385726
version: 1
top level: BBa_K1333200
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1333200/annotation2385726/range2385726/1

https://synbiohub.org/public/igem/BBa_K1333200/annotation2385726/range2385726/1
type: Range
persistent identity: range2385726
display id: range2385726
version: 1
direction: direction/forward
top level: BBa_K1333200
start: 721
end: 840
orientation: inline

lambda cI
type: SequenceAnnotation
persistent identity: annotation2385724
display id: annotation2385724
version: 1
top level: BBa_K1333200
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1333200/annotation2385724/range2385724/1

https://synbiohub.org/public/igem/BBa_K1333200/annotation2385724/range2385724/1
type: Range
persistent identity: range2385724
display id: range2385724
version: 1
direction: direction/forward
top level: BBa_K1333200
start: 1
end: 711
orientation: inline

ATG start codon
type: SequenceAnnotation
persistent identity: annotation2385725
display id: annotation2385725
version: 1
top level: BBa_K1333200
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1333200/annotation2385725/range2385725/1

https://synbiohub.org/public/igem/BBa_K1333200/annotation2385725/range2385725/1
type: Range
persistent identity: range2385725
display id: range2385725
version: 1
direction: direction/forward
top level: BBa_K1333200
start: 1
end: 3
orientation: inline

TGA stop codon
type: SequenceAnnotation
persistent identity: annotation2385727
display id: annotation2385727
version: 1
top level: BBa_K1333200
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1333200/annotation2385727/range2385727/1

https://synbiohub.org/public/igem/BBa_K1333200/annotation2385727/range2385727/1
type: Range
persistent identity: range2385727
display id: range2385727
version: 1
direction: direction/forward
top level: BBa_K1333200
start: 841
end: 843
orientation: inline

BBa_K404305
type: ComponentDefinition
persistent identity: BBa K404305
display id: BBa_K404305
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Z-EGFR-1907_Long-Linker
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: Long-linker
sequence annotation: Z-EGFR-1907
sequence: https://synbiohub.org/public/igem/BBa_K404305_sequence/1

Long-linker
type: SequenceAnnotation
persistent identity: annotation2094757
display id: annotation2094757
version: 1
top level: BBa_K404305
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404305/annotation2094757/range2094757/1

https://synbiohub.org/public/igem/BBa_K404305/annotation2094757/range2094757/1
type: Range
persistent identity: range2094757
display id: range2094757
version: 1
direction: direction/forward
top level: BBa_K404305
start: 181
end: 216
orientation: inline

Z-EGFR-1907
type: SequenceAnnotation
persistent identity: annotation2094756
display id: annotation2094756
version: 1
top level: BBa_K404305
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404305/annotation2094756/range2094756/1

https://synbiohub.org/public/igem/BBa_K404305/annotation2094756/range2094756/1
type: Range
persistent identity: range2094756
display id: range2094756
version: 1
direction: direction/forward
top level: BBa_K404305
start: 1
end: 175
orientation: inline

BBa_K157004
type: ComponentDefinition
persistent identity: BBa K157004
display id: BBa_K157004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fluoresceine-A-binding
creator: iGEM Team Freiburg 2008
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Fluoresceine A -binding derivative of a Lipocalin (BBP, bilin binding protein from pieris brassicae), originally designed by Arne Skerra [see Ref. 1,2].
mutable notes: Between BioBrick 1.0 sites this part is flanked 5' by NgoMI(=NgoMIV) and 3' AgeI(=PinAI) to facilitate in frame cloning for protein fusions.
mutable provenance: Gene synthesis by GeneArt, optimized for expression in homo sapiens.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: Lipocalin FluA
sequence: https://synbiohub.org/public/igem/BBa_K157004_sequence/1

Lipocalin FluA
type: SequenceAnnotation
persistent identity: annotation2040659
display id: annotation2040659
version: 1
top level: BBa_K157004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157004/annotation2040659/range2040659/1

https://synbiohub.org/public/igem/BBa_K157004/annotation2040659/range2040659/1
type: Range
persistent identity: range2040659
display id: range2040659
version: 1
direction: direction/forward
top level: BBa_K157004
start: 1
end: 522
orientation: inline

BBa_J18952
type: ComponentDefinition
persistent identity: BBa J18952
display id: BBa_J18952
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FRB ~ mCerulean
creator: Raik Gruenberg
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
discontinued: false
dominant: false
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: P20 expression cassette from Gruenberg et al. 2010 series of interaction reporter proteins. All proteins share the same architecture: * T7Start-domain1-5xGS-domain2-preSCsite-HisTag-T7Stop Only Domains1 and 2 vary and are taken from a list of protein interaction input and output (readout) parts. ===Expression=== Proteins were expressed in E. coli BL21 using T7 Polymerase and were purified by His-tag affinity. ===Reference=== * Gruenberg, Ferrar, van der Sloot, Serrano (2010): Building Blocks for Protein Interaction Devices
mutable notes: Constructs were assembled according to R.F.C. 25. All parts were codon optimized for E. coli.
mutable provenance: synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_J18919
component: BBa_B0105
component: BBa_J18922
component: BBa_J18909
component: BBa_J18930
component: BBa_J18913
component: BBa_J18912
component: BBa_J18926
sequence annotation: BBa_J18909
sequence annotation: BBa_B0105
sequence annotation: BBa_J18930
sequence annotation: BBa_J18912
sequence annotation: BBa_J18913
sequence annotation: BBa_J18922
sequence annotation: BBa_J18926
sequence annotation: BBa_J18919
sequence: https://synbiohub.org/public/igem/BBa_J18952_sequence/1

BBa_J18919
type: Component
persistent identity: component2066816
display id: component2066816
version: 1
top level: BBa_J18952
access: public
definition: https://synbiohub.org/public/igem/BBa_J18919/1

BBa_B0105
type: Component
persistent identity: component2066812
display id: component2066812
version: 1
top level: BBa_J18952
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_J18922
type: Component
persistent identity: component2066810
display id: component2066810
version: 1
top level: BBa_J18952
access: public
definition: https://synbiohub.org/public/igem/BBa_J18922/1

BBa_J18909
type: Component
persistent identity: component2066820
display id: component2066820
version: 1
top level: BBa_J18952
access: public
definition: https://synbiohub.org/public/igem/BBa_J18909/1

BBa_B0105
type: Component
persistent identity: component2066822
display id: component2066822
version: 1
top level: BBa_J18952
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_B0105
type: Component
persistent identity: component2066809
display id: component2066809
version: 1
top level: BBa_J18952
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_J18930
type: Component
persistent identity: component2066813
display id: component2066813
version: 1
top level: BBa_J18952
access: public
definition: https://synbiohub.org/public/igem/BBa_J18930/1

BBa_J18913
type: Component
persistent identity: component2066824
display id: component2066824
version: 1
top level: BBa_J18952
access: public
definition: https://synbiohub.org/public/igem/BBa_J18913/1

BBa_B0105
type: Component
persistent identity: component2066815
display id: component2066815
version: 1
top level: BBa_J18952
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_B0105
type: Component
persistent identity: component2066818
display id: component2066818
version: 1
top level: BBa_J18952
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_J18912
type: Component
persistent identity: component2066804
display id: component2066804
version: 1
top level: BBa_J18952
access: public
definition: https://synbiohub.org/public/igem/BBa_J18912/1

BBa_J18926
type: Component
persistent identity: component2066807
display id: component2066807
version: 1
top level: BBa_J18952
access: public
definition: FRB

BBa_B0105
type: Component
persistent identity: component2066806
display id: component2066806
version: 1
top level: BBa_J18952
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_J18909
type: SequenceAnnotation
persistent identity: annotation2066820
display id: annotation2066820
version: 1
top level: BBa_J18952
location: https://synbiohub.org/public/igem/BBa_J18952/annotation2066820/range2066820/1
component: BBa_J18909

https://synbiohub.org/public/igem/BBa_J18952/annotation2066820/range2066820/1
type: Range
persistent identity: range2066820
display id: range2066820
version: 1
direction: direction/reverse
top level: BBa_J18952
start: 1161
end: 1178
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066818
display id: annotation2066818
version: 1
top level: BBa_J18952
location: https://synbiohub.org/public/igem/BBa_J18952/annotation2066818/range2066818/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18952/annotation2066818/range2066818/1
type: Range
persistent identity: range2066818
display id: range2066818
version: 1
direction: direction/reverse
top level: BBa_J18952
start: 1155
end: 1160
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066812
display id: annotation2066812
version: 1
top level: BBa_J18952
location: https://synbiohub.org/public/igem/BBa_J18952/annotation2066812/range2066812/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18952/annotation2066812/range2066812/1
type: Range
persistent identity: range2066812
display id: range2066812
version: 1
direction: direction/reverse
top level: BBa_J18952
start: 405
end: 410
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066809
display id: annotation2066809
version: 1
top level: BBa_J18952
location: https://synbiohub.org/public/igem/BBa_J18952/annotation2066809/range2066809/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18952/annotation2066809/range2066809/1
type: Range
persistent identity: range2066809
display id: range2066809
version: 1
direction: direction/reverse
top level: BBa_J18952
start: 369
end: 374
orientation: inline

BBa_J18930
type: SequenceAnnotation
persistent identity: annotation2066813
display id: annotation2066813
version: 1
top level: BBa_J18952
location: https://synbiohub.org/public/igem/BBa_J18952/annotation2066813/range2066813/1
component: BBa_J18930

https://synbiohub.org/public/igem/BBa_J18952/annotation2066813/range2066813/1
type: Range
persistent identity: range2066813
display id: range2066813
version: 1
direction: direction/reverse
top level: BBa_J18952
start: 411
end: 1124
orientation: inline

BBa_J18912
type: SequenceAnnotation
persistent identity: annotation2066804
display id: annotation2066804
version: 1
top level: BBa_J18952
location: https://synbiohub.org/public/igem/BBa_J18952/annotation2066804/range2066804/1
component: BBa_J18912

https://synbiohub.org/public/igem/BBa_J18952/annotation2066804/range2066804/1
type: Range
persistent identity: range2066804
display id: range2066804
version: 1
direction: direction/reverse
top level: BBa_J18952
start: 1
end: 83
orientation: inline

BBa_J18913
type: SequenceAnnotation
persistent identity: annotation2066824
display id: annotation2066824
version: 1
top level: BBa_J18952
location: https://synbiohub.org/public/igem/BBa_J18952/annotation2066824/range2066824/1
component: BBa_J18913

https://synbiohub.org/public/igem/BBa_J18952/annotation2066824/range2066824/1
type: Range
persistent identity: range2066824
display id: range2066824
version: 1
direction: direction/reverse
top level: BBa_J18952
start: 1185
end: 1319
orientation: inline

BBa_J18922
type: SequenceAnnotation
persistent identity: annotation2066810
display id: annotation2066810
version: 1
top level: BBa_J18952
location: https://synbiohub.org/public/igem/BBa_J18952/annotation2066810/range2066810/1
component: BBa_J18922

https://synbiohub.org/public/igem/BBa_J18952/annotation2066810/range2066810/1
type: Range
persistent identity: range2066810
display id: range2066810
version: 1
direction: direction/reverse
top level: BBa_J18952
start: 375
end: 404
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066806
display id: annotation2066806
version: 1
top level: BBa_J18952
location: https://synbiohub.org/public/igem/BBa_J18952/annotation2066806/range2066806/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18952/annotation2066806/range2066806/1
type: Range
persistent identity: range2066806
display id: range2066806
version: 1
direction: direction/reverse
top level: BBa_J18952
start: 84
end: 89
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066822
display id: annotation2066822
version: 1
top level: BBa_J18952
location: https://synbiohub.org/public/igem/BBa_J18952/annotation2066822/range2066822/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18952/annotation2066822/range2066822/1
type: Range
persistent identity: range2066822
display id: range2066822
version: 1
direction: direction/reverse
top level: BBa_J18952
start: 1179
end: 1184
orientation: inline

BBa_J18926
type: SequenceAnnotation
persistent identity: annotation2066807
display id: annotation2066807
version: 1
top level: BBa_J18952
location: https://synbiohub.org/public/igem/BBa_J18952/annotation2066807/range2066807/1
component: BBa_J18926

https://synbiohub.org/public/igem/BBa_J18952/annotation2066807/range2066807/1
type: Range
persistent identity: range2066807
display id: range2066807
version: 1
direction: direction/reverse
top level: BBa_J18952
start: 90
end: 368
orientation: inline

BBa_J18919
type: SequenceAnnotation
persistent identity: annotation2066816
display id: annotation2066816
version: 1
top level: BBa_J18952
location: https://synbiohub.org/public/igem/BBa_J18952/annotation2066816/range2066816/1
component: BBa_J18919

https://synbiohub.org/public/igem/BBa_J18952/annotation2066816/range2066816/1
type: Range
persistent identity: range2066816
display id: range2066816
version: 1
direction: direction/reverse
top level: BBa_J18952
start: 1131
end: 1154
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066815
display id: annotation2066815
version: 1
top level: BBa_J18952
location: https://synbiohub.org/public/igem/BBa_J18952/annotation2066815/range2066815/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18952/annotation2066815/range2066815/1
type: Range
persistent identity: range2066815
display id: range2066815
version: 1
direction: direction/reverse
top level: BBa_J18952
start: 1125
end: 1130
orientation: inline

Lov2
type: ComponentDefinition
persistent identity: BBa J18927
display id: BBa_J18927
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: phot1-LOV2
creator: Raik Gruenberg
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The second LOV domain of Arabidopsis phototropin 1 shows light-induced transient (30s) homo-dimerization between a ground state and an excited monomer (Nakasone et al. 2006). LOV2 is predominantly monomeric at concentrations below 100microM but turns dimeric above. Funnily, the high-conc. dimer state experiences photo-dissociation instead. Either the excitation follows a different course from the dimer starting state or the excitation event itself pushes the complex apart. (CRG-internal ID: rg0090) ===References=== * Nakasone et al. 2006 PMID: 16679373 * Freddolino, Peter L.; Dittrich, Markus; Schulten, Klaus (2006) Dynamic Switching Mechanisms in LOV1 and LOV2 Domains of Plant Phototropins PMID: 16935961
mutable notes: The fragment used by Nakasone et al. is 475K - 578G. The core domain 449E - 586R has reportedly lower stability at RT (aggregation). * gene synthesis * codon optimized for E. coli
mutable provenance: literature gene synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_J18927_sequence/1

BBa_K157003
type: ComponentDefinition
persistent identity: BBa K157003
display id: BBa_K157003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anti-NIP singlechain Fv-Fragment
creator: iGEM Team Freiburg 2008
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Singlechain Fv.Fragment of an Anti-Nitro-Iodo-Phenol-antibody B1-8. Binds the hapten NIP (Nitro-Iodo-Phenole) with an affinity of 2.0 uM[1,2]. Designed for fusion to proteins or peptides via in frame cloning.
mutable notes: Between BioBrick 1.0 sites this part is flanked 5' by NgoMI(=NgoMIV) and 3' AgeI(=PinAI) to facilitate in frame cloning for protein fusions.
mutable provenance: Gene synthesis by GeneArt, optimized for expression in homo sapiens.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: Anti-NIP sc Fv-fragment
sequence: https://synbiohub.org/public/igem/BBa_K157003_sequence/1

Anti-NIP sc Fv-fragment
type: SequenceAnnotation
persistent identity: annotation2064690
display id: annotation2064690
version: 1
top level: BBa_K157003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157003/annotation2064690/range2064690/1

https://synbiohub.org/public/igem/BBa_K157003/annotation2064690/range2064690/1
type: Range
persistent identity: range2064690
display id: range2064690
version: 1
direction: direction/forward
top level: BBa_K157003
start: 1
end: 735
orientation: inline

BBa_K404302
type: ComponentDefinition
persistent identity: BBa K404302
display id: BBa_K404302
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Z-EGFR-1907
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: Z-EGFR-1907
sequence: https://synbiohub.org/public/igem/BBa_K404302_sequence/1

Z-EGFR-1907
type: SequenceAnnotation
persistent identity: annotation2094740
display id: annotation2094740
version: 1
top level: BBa_K404302
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404302/annotation2094740/range2094740/1

https://synbiohub.org/public/igem/BBa_K404302/annotation2094740/range2094740/1
type: Range
persistent identity: range2094740
display id: range2094740
version: 1
direction: direction/forward
top level: BBa_K404302
start: 1
end: 174
orientation: inline

BBa_K404304
type: ComponentDefinition
persistent identity: BBa K404304
display id: BBa_K404304
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Z-EGFR-1907_Middle-Linker
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: Z-EGFR-1907
sequence annotation: Middle-linker
sequence: https://synbiohub.org/public/igem/BBa_K404304_sequence/1

Z-EGFR-1907
type: SequenceAnnotation
persistent identity: annotation2094745
display id: annotation2094745
version: 1
top level: BBa_K404304
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404304/annotation2094745/range2094745/1

https://synbiohub.org/public/igem/BBa_K404304/annotation2094745/range2094745/1
type: Range
persistent identity: range2094745
display id: range2094745
version: 1
direction: direction/forward
top level: BBa_K404304
start: 1
end: 175
orientation: inline

Middle-linker
type: SequenceAnnotation
persistent identity: annotation2094746
display id: annotation2094746
version: 1
top level: BBa_K404304
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404304/annotation2094746/range2094746/1

https://synbiohub.org/public/igem/BBa_K404304/annotation2094746/range2094746/1
type: Range
persistent identity: range2094746
display id: range2094746
version: 1
direction: direction/forward
top level: BBa_K404304
start: 181
end: 204
orientation: inline

CBDcex(J61
type: ComponentDefinition
persistent identity: BBa K863104
display id: BBa_K863104
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cellulose binding Domain from C. Fimi with const. Promotor and GFP
creator: Moritz M??ller
created: 2012-09-20T11:00:00Z
modified: 2015-06-17T01:19:39Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1123_
m user id: 4206
owner id: 13080
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Cellulose binding Domain from Cellulomonas Fimi with const. Promotor J61101 and Reporter GFP
mutable notes: a switch to a const. Promoter
mutable provenance: different
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: BBa_J23100
sequence annotation: Freiburg-Scar
sequence annotation: Cellulose binding domain (CBDcex)
sequence annotation: BBa_K863101
sequence annotation: BBa_K863121
sequence annotation: TAA
sequence annotation: Exoglucanase
sequence annotation: GS-Linker
sequence annotation: ATG
sequence annotation: AgeI
sequence annotation: BBa_J61101
sequence annotation: BBa_E0040
sequence annotation: NgoMIV
sequence annotation: His-Tag
sequence: https://synbiohub.org/public/igem/BBa_K863104_sequence/1

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2189483
display id: annotation2189483
version: 1
top level: CBDcex(J61
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189483/range2189483/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189483/range2189483/1
type: Range
persistent identity: range2189483
display id: range2189483
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 1
end: 35
orientation: inline

Freiburg-Scar
type: SequenceAnnotation
persistent identity: annotation2189492
display id: annotation2189492
version: 1
top level: CBDcex(J61
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189492/range2189492/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189492/range2189492/1
type: Range
persistent identity: range2189492
display id: range2189492
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 398
end: 403
orientation: inline

Cellulose binding domain (CBDcex)
type: SequenceAnnotation
persistent identity: annotation2189489
display id: annotation2189489
version: 1
top level: CBDcex(J61
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189489/range2189489/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189489/range2189489/1
type: Range
persistent identity: range2189489
display id: range2189489
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 83
end: 382
orientation: inline

BBa_K863101
type: SequenceAnnotation
persistent identity: annotation2189486
display id: annotation2189486
version: 1
top level: CBDcex(J61
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189486/range2189486/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189486/range2189486/1
type: Range
persistent identity: range2189486
display id: range2189486
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 62
end: 397
orientation: inline

BBa_K863121
type: SequenceAnnotation
persistent identity: annotation2189493
display id: annotation2189493
version: 1
top level: CBDcex(J61
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189493/range2189493/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189493/range2189493/1
type: Range
persistent identity: range2189493
display id: range2189493
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 404
end: 1144
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2189497
display id: annotation2189497
version: 1
top level: CBDcex(J61
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189497/range2189497/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189497/range2189497/1
type: Range
persistent identity: range2189497
display id: range2189497
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 1142
end: 1144
orientation: inline

Exoglucanase
type: SequenceAnnotation
persistent identity: annotation2189490
display id: annotation2189490
version: 1
top level: CBDcex(J61
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189490/range2189490/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189490/range2189490/1
type: Range
persistent identity: range2189490
display id: range2189490
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 383
end: 391
orientation: inline

GS-Linker
type: SequenceAnnotation
persistent identity: annotation2189491
display id: annotation2189491
version: 1
top level: CBDcex(J61
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189491/range2189491/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189491/range2189491/1
type: Range
persistent identity: range2189491
display id: range2189491
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 392
end: 397
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2189485
display id: annotation2189485
version: 1
top level: CBDcex(J61
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189485/range2189485/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189485/range2189485/1
type: Range
persistent identity: range2189485
display id: range2189485
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 62
end: 64
orientation: inline

Exoglucanase
type: SequenceAnnotation
persistent identity: annotation2189488
display id: annotation2189488
version: 1
top level: CBDcex(J61
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189488/range2189488/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189488/range2189488/1
type: Range
persistent identity: range2189488
display id: range2189488
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 71
end: 82
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2189496
display id: annotation2189496
version: 1
top level: CBDcex(J61
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189496/range2189496/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189496/range2189496/1
type: Range
persistent identity: range2189496
display id: range2189496
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 1136
end: 1141
orientation: inline

BBa_J61101
type: SequenceAnnotation
persistent identity: annotation2189484
display id: annotation2189484
version: 1
top level: CBDcex(J61
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189484/range2189484/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189484/range2189484/1
type: Range
persistent identity: range2189484
display id: range2189484
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 44
end: 55
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2189494
display id: annotation2189494
version: 1
top level: CBDcex(J61
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189494/range2189494/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189494/range2189494/1
type: Range
persistent identity: range2189494
display id: range2189494
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 404
end: 1117
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2189487
display id: annotation2189487
version: 1
top level: CBDcex(J61
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189487/range2189487/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189487/range2189487/1
type: Range
persistent identity: range2189487
display id: range2189487
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 65
end: 70
orientation: inline

His-Tag
type: SequenceAnnotation
persistent identity: annotation2189495
display id: annotation2189495
version: 1
top level: CBDcex(J61
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189495/range2189495/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189495/range2189495/1
type: Range
persistent identity: range2189495
display id: range2189495
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 1118
end: 1135
orientation: inline

BBa_J18949
type: ComponentDefinition
persistent identity: BBa J18949
display id: BBa_J18949
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GLucFrag1 ~ Lov2
creator: Raik Gruenberg
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
discontinued: false
dominant: false
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: P17 expression cassette from Gruenberg et al. 2010 series of interaction reporter proteins. All proteins share the same architecture: * T7Start-domain1-5xGS-domain2-preSCsite-HisTag-T7Stop Only Domains1 and 2 vary and are taken from a list of protein interaction input and output (readout) parts. ===Expression=== Proteins were expressed in E. coli BL21 using T7 Polymerase and were purified by His-tag affinity. ===Reference=== * Gruenberg, Ferrar, van der Sloot, Serrano (2010): Building Blocks for Protein Interaction Devices
mutable notes: Constructs were assembled according to R.F.C. 25. All parts were codon optimized for E. coli.
mutable provenance: synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0105
component: BBa_J18922
component: BBa_J18927
component: BBa_J18928
component: BBa_J18909
component: BBa_J18919
component: BBa_J18912
component: BBa_J18913
sequence annotation: BBa_B0105
sequence annotation: BBa_J18912
sequence annotation: BBa_J18922
sequence annotation: BBa_J18927
sequence annotation: BBa_J18928
sequence annotation: BBa_J18913
sequence annotation: BBa_J18909
sequence annotation: BBa_J18919
sequence: https://synbiohub.org/public/igem/BBa_J18949_sequence/1

BBa_B0105
type: Component
persistent identity: component2066756
display id: component2066756
version: 1
top level: BBa_J18949
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_J18922
type: Component
persistent identity: component2066744
display id: component2066744
version: 1
top level: BBa_J18949
access: public
definition: https://synbiohub.org/public/igem/BBa_J18922/1

BBa_J18927
type: Component
persistent identity: component2066747
display id: component2066747
version: 1
top level: BBa_J18949
access: public
definition: Lov2

BBa_J18928
type: Component
persistent identity: component2066741
display id: component2066741
version: 1
top level: BBa_J18949
access: public
definition: GLucFrag1

BBa_J18909
type: Component
persistent identity: component2066754
display id: component2066754
version: 1
top level: BBa_J18949
access: public
definition: https://synbiohub.org/public/igem/BBa_J18909/1

BBa_J18919
type: Component
persistent identity: component2066750
display id: component2066750
version: 1
top level: BBa_J18949
access: public
definition: https://synbiohub.org/public/igem/BBa_J18919/1

BBa_B0105
type: Component
persistent identity: component2066740
display id: component2066740
version: 1
top level: BBa_J18949
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_B0105
type: Component
persistent identity: component2066752
display id: component2066752
version: 1
top level: BBa_J18949
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_B0105
type: Component
persistent identity: component2066743
display id: component2066743
version: 1
top level: BBa_J18949
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_B0105
type: Component
persistent identity: component2066746
display id: component2066746
version: 1
top level: BBa_J18949
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_J18912
type: Component
persistent identity: component2066738
display id: component2066738
version: 1
top level: BBa_J18949
access: public
definition: https://synbiohub.org/public/igem/BBa_J18912/1

BBa_J18913
type: Component
persistent identity: component2066758
display id: component2066758
version: 1
top level: BBa_J18949
access: public
definition: https://synbiohub.org/public/igem/BBa_J18913/1

BBa_B0105
type: Component
persistent identity: component2066749
display id: component2066749
version: 1
top level: BBa_J18949
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066740
display id: annotation2066740
version: 1
top level: BBa_J18949
location: https://synbiohub.org/public/igem/BBa_J18949/annotation2066740/range2066740/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18949/annotation2066740/range2066740/1
type: Range
persistent identity: range2066740
display id: range2066740
version: 1
direction: direction/reverse
top level: BBa_J18949
start: 84
end: 89
orientation: inline

BBa_J18912
type: SequenceAnnotation
persistent identity: annotation2066738
display id: annotation2066738
version: 1
top level: BBa_J18949
location: https://synbiohub.org/public/igem/BBa_J18949/annotation2066738/range2066738/1
component: BBa_J18912

https://synbiohub.org/public/igem/BBa_J18949/annotation2066738/range2066738/1
type: Range
persistent identity: range2066738
display id: range2066738
version: 1
direction: direction/reverse
top level: BBa_J18949
start: 1
end: 83
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066756
display id: annotation2066756
version: 1
top level: BBa_J18949
location: https://synbiohub.org/public/igem/BBa_J18949/annotation2066756/range2066756/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18949/annotation2066756/range2066756/1
type: Range
persistent identity: range2066756
display id: range2066756
version: 1
direction: direction/reverse
top level: BBa_J18949
start: 876
end: 881
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066743
display id: annotation2066743
version: 1
top level: BBa_J18949
location: https://synbiohub.org/public/igem/BBa_J18949/annotation2066743/range2066743/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18949/annotation2066743/range2066743/1
type: Range
persistent identity: range2066743
display id: range2066743
version: 1
direction: direction/reverse
top level: BBa_J18949
start: 366
end: 371
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066749
display id: annotation2066749
version: 1
top level: BBa_J18949
location: https://synbiohub.org/public/igem/BBa_J18949/annotation2066749/range2066749/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18949/annotation2066749/range2066749/1
type: Range
persistent identity: range2066749
display id: range2066749
version: 1
direction: direction/reverse
top level: BBa_J18949
start: 822
end: 827
orientation: inline

BBa_J18922
type: SequenceAnnotation
persistent identity: annotation2066744
display id: annotation2066744
version: 1
top level: BBa_J18949
location: https://synbiohub.org/public/igem/BBa_J18949/annotation2066744/range2066744/1
component: BBa_J18922

https://synbiohub.org/public/igem/BBa_J18949/annotation2066744/range2066744/1
type: Range
persistent identity: range2066744
display id: range2066744
version: 1
direction: direction/reverse
top level: BBa_J18949
start: 372
end: 401
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066746
display id: annotation2066746
version: 1
top level: BBa_J18949
location: https://synbiohub.org/public/igem/BBa_J18949/annotation2066746/range2066746/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18949/annotation2066746/range2066746/1
type: Range
persistent identity: range2066746
display id: range2066746
version: 1
direction: direction/reverse
top level: BBa_J18949
start: 402
end: 407
orientation: inline

BBa_J18927
type: SequenceAnnotation
persistent identity: annotation2066747
display id: annotation2066747
version: 1
top level: BBa_J18949
location: https://synbiohub.org/public/igem/BBa_J18949/annotation2066747/range2066747/1
component: BBa_J18927

https://synbiohub.org/public/igem/BBa_J18949/annotation2066747/range2066747/1
type: Range
persistent identity: range2066747
display id: range2066747
version: 1
direction: direction/reverse
top level: BBa_J18949
start: 408
end: 821
orientation: inline

BBa_J18928
type: SequenceAnnotation
persistent identity: annotation2066741
display id: annotation2066741
version: 1
top level: BBa_J18949
location: https://synbiohub.org/public/igem/BBa_J18949/annotation2066741/range2066741/1
component: BBa_J18928

https://synbiohub.org/public/igem/BBa_J18949/annotation2066741/range2066741/1
type: Range
persistent identity: range2066741
display id: range2066741
version: 1
direction: direction/reverse
top level: BBa_J18949
start: 90
end: 365
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066752
display id: annotation2066752
version: 1
top level: BBa_J18949
location: https://synbiohub.org/public/igem/BBa_J18949/annotation2066752/range2066752/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18949/annotation2066752/range2066752/1
type: Range
persistent identity: range2066752
display id: range2066752
version: 1
direction: direction/reverse
top level: BBa_J18949
start: 852
end: 857
orientation: inline

BBa_J18913
type: SequenceAnnotation
persistent identity: annotation2066758
display id: annotation2066758
version: 1
top level: BBa_J18949
location: https://synbiohub.org/public/igem/BBa_J18949/annotation2066758/range2066758/1
component: BBa_J18913

https://synbiohub.org/public/igem/BBa_J18949/annotation2066758/range2066758/1
type: Range
persistent identity: range2066758
display id: range2066758
version: 1
direction: direction/reverse
top level: BBa_J18949
start: 882
end: 1016
orientation: inline

BBa_J18909
type: SequenceAnnotation
persistent identity: annotation2066754
display id: annotation2066754
version: 1
top level: BBa_J18949
location: https://synbiohub.org/public/igem/BBa_J18949/annotation2066754/range2066754/1
component: BBa_J18909

https://synbiohub.org/public/igem/BBa_J18949/annotation2066754/range2066754/1
type: Range
persistent identity: range2066754
display id: range2066754
version: 1
direction: direction/reverse
top level: BBa_J18949
start: 858
end: 875
orientation: inline

BBa_J18919
type: SequenceAnnotation
persistent identity: annotation2066750
display id: annotation2066750
version: 1
top level: BBa_J18949
location: https://synbiohub.org/public/igem/BBa_J18949/annotation2066750/range2066750/1
component: BBa_J18919

https://synbiohub.org/public/igem/BBa_J18949/annotation2066750/range2066750/1
type: Range
persistent identity: range2066750
display id: range2066750
version: 1
direction: direction/reverse
top level: BBa_J18949
start: 828
end: 851
orientation: inline

BBa_J18934
type: ComponentDefinition
persistent identity: BBa J18934
display id: BBa_J18934
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FKBP~GLucFrag2
creator: Raik Gruenberg
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
discontinued: false
dominant: false
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: P02 expression cassette from Gruenberg et al. 2010 series of interaction reporter proteins. All proteins share the same architecture: * T7Start-domain1-5xGS-domain2-preSCsite-HisTag-T7Stop Only Domains1 and 2 vary and are taken from a list of protein interaction input and output (readout) parts. ===Expression=== Proteins were expressed in E. coli BL21 using T7 Polymerase and were purified by His-tag affinity. ===Reference=== * Gruenberg, Ferrar, van der Sloot, Serrano (2010): Building Blocks for Protein Interaction Devices
mutable notes: Constructs were assembled according to R.F.C. 25 All parts were codon optimized for E. coli
mutable provenance: synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_J18909
component: BBa_B0105
component: BBa_J18922
component: BBa_J18913
component: BBa_J18912
component: BBa_J18925
component: BBa_J18919
component: BBa_J18929
sequence annotation: BBa_J18919
sequence annotation: BBa_J18925
sequence annotation: BBa_B0105
sequence annotation: BBa_J18913
sequence annotation: BBa_J18912
sequence annotation: BBa_J18922
sequence annotation: BBa_J18929
sequence annotation: BBa_J18909
sequence: https://synbiohub.org/public/igem/BBa_J18934_sequence/1

BBa_J18909
type: Component
persistent identity: component2066437
display id: component2066437
version: 1
top level: BBa_J18934
access: public
definition: https://synbiohub.org/public/igem/BBa_J18909/1

BBa_B0105
type: Component
persistent identity: component2066426
display id: component2066426
version: 1
top level: BBa_J18934
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_J18922
type: Component
persistent identity: component2066427
display id: component2066427
version: 1
top level: BBa_J18934
access: public
definition: https://synbiohub.org/public/igem/BBa_J18922/1

BBa_J18913
type: Component
persistent identity: component2066441
display id: component2066441
version: 1
top level: BBa_J18934
access: public
definition: https://synbiohub.org/public/igem/BBa_J18913/1

BBa_J18912
type: Component
persistent identity: component2066421
display id: component2066421
version: 1
top level: BBa_J18934
access: public
definition: https://synbiohub.org/public/igem/BBa_J18912/1

BBa_B0105
type: Component
persistent identity: component2066429
display id: component2066429
version: 1
top level: BBa_J18934
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_J18925
type: Component
persistent identity: component2066424
display id: component2066424
version: 1
top level: BBa_J18934
access: public
definition: FKBP12

BBa_J18919
type: Component
persistent identity: component2066433
display id: component2066433
version: 1
top level: BBa_J18934
access: public
definition: https://synbiohub.org/public/igem/BBa_J18919/1

BBa_B0105
type: Component
persistent identity: component2066423
display id: component2066423
version: 1
top level: BBa_J18934
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_B0105
type: Component
persistent identity: component2066439
display id: component2066439
version: 1
top level: BBa_J18934
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_B0105
type: Component
persistent identity: component2066435
display id: component2066435
version: 1
top level: BBa_J18934
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_B0105
type: Component
persistent identity: component2066432
display id: component2066432
version: 1
top level: BBa_J18934
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_J18929
type: Component
persistent identity: component2066430
display id: component2066430
version: 1
top level: BBa_J18934
access: public
definition: GLucFrag2

BBa_J18919
type: SequenceAnnotation
persistent identity: annotation2066433
display id: annotation2066433
version: 1
top level: BBa_J18934
location: https://synbiohub.org/public/igem/BBa_J18934/annotation2066433/range2066433/1
component: BBa_J18919

https://synbiohub.org/public/igem/BBa_J18934/annotation2066433/range2066433/1
type: Range
persistent identity: range2066433
display id: range2066433
version: 1
direction: direction/reverse
top level: BBa_J18934
start: 687
end: 710
orientation: inline

BBa_J18925
type: SequenceAnnotation
persistent identity: annotation2066424
display id: annotation2066424
version: 1
top level: BBa_J18934
location: https://synbiohub.org/public/igem/BBa_J18934/annotation2066424/range2066424/1
component: BBa_J18925

https://synbiohub.org/public/igem/BBa_J18934/annotation2066424/range2066424/1
type: Range
persistent identity: range2066424
display id: range2066424
version: 1
direction: direction/reverse
top level: BBa_J18934
start: 90
end: 410
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066429
display id: annotation2066429
version: 1
top level: BBa_J18934
location: https://synbiohub.org/public/igem/BBa_J18934/annotation2066429/range2066429/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18934/annotation2066429/range2066429/1
type: Range
persistent identity: range2066429
display id: range2066429
version: 1
direction: direction/reverse
top level: BBa_J18934
start: 447
end: 452
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066432
display id: annotation2066432
version: 1
top level: BBa_J18934
location: https://synbiohub.org/public/igem/BBa_J18934/annotation2066432/range2066432/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18934/annotation2066432/range2066432/1
type: Range
persistent identity: range2066432
display id: range2066432
version: 1
direction: direction/reverse
top level: BBa_J18934
start: 681
end: 686
orientation: inline

BBa_J18913
type: SequenceAnnotation
persistent identity: annotation2066441
display id: annotation2066441
version: 1
top level: BBa_J18934
location: https://synbiohub.org/public/igem/BBa_J18934/annotation2066441/range2066441/1
component: BBa_J18913

https://synbiohub.org/public/igem/BBa_J18934/annotation2066441/range2066441/1
type: Range
persistent identity: range2066441
display id: range2066441
version: 1
direction: direction/reverse
top level: BBa_J18934
start: 741
end: 875
orientation: inline

BBa_J18912
type: SequenceAnnotation
persistent identity: annotation2066421
display id: annotation2066421
version: 1
top level: BBa_J18934
location: https://synbiohub.org/public/igem/BBa_J18934/annotation2066421/range2066421/1
component: BBa_J18912

https://synbiohub.org/public/igem/BBa_J18934/annotation2066421/range2066421/1
type: Range
persistent identity: range2066421
display id: range2066421
version: 1
direction: direction/reverse
top level: BBa_J18934
start: 1
end: 83
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066426
display id: annotation2066426
version: 1
top level: BBa_J18934
location: https://synbiohub.org/public/igem/BBa_J18934/annotation2066426/range2066426/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18934/annotation2066426/range2066426/1
type: Range
persistent identity: range2066426
display id: range2066426
version: 1
direction: direction/reverse
top level: BBa_J18934
start: 411
end: 416
orientation: inline

BBa_J18922
type: SequenceAnnotation
persistent identity: annotation2066427
display id: annotation2066427
version: 1
top level: BBa_J18934
location: https://synbiohub.org/public/igem/BBa_J18934/annotation2066427/range2066427/1
component: BBa_J18922

https://synbiohub.org/public/igem/BBa_J18934/annotation2066427/range2066427/1
type: Range
persistent identity: range2066427
display id: range2066427
version: 1
direction: direction/reverse
top level: BBa_J18934
start: 417
end: 446
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066423
display id: annotation2066423
version: 1
top level: BBa_J18934
location: https://synbiohub.org/public/igem/BBa_J18934/annotation2066423/range2066423/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18934/annotation2066423/range2066423/1
type: Range
persistent identity: range2066423
display id: range2066423
version: 1
direction: direction/reverse
top level: BBa_J18934
start: 84
end: 89
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066435
display id: annotation2066435
version: 1
top level: BBa_J18934
location: https://synbiohub.org/public/igem/BBa_J18934/annotation2066435/range2066435/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18934/annotation2066435/range2066435/1
type: Range
persistent identity: range2066435
display id: range2066435
version: 1
direction: direction/reverse
top level: BBa_J18934
start: 711
end: 716
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066439
display id: annotation2066439
version: 1
top level: BBa_J18934
location: https://synbiohub.org/public/igem/BBa_J18934/annotation2066439/range2066439/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18934/annotation2066439/range2066439/1
type: Range
persistent identity: range2066439
display id: range2066439
version: 1
direction: direction/reverse
top level: BBa_J18934
start: 735
end: 740
orientation: inline

BBa_J18929
type: SequenceAnnotation
persistent identity: annotation2066430
display id: annotation2066430
version: 1
top level: BBa_J18934
location: https://synbiohub.org/public/igem/BBa_J18934/annotation2066430/range2066430/1
component: BBa_J18929

https://synbiohub.org/public/igem/BBa_J18934/annotation2066430/range2066430/1
type: Range
persistent identity: range2066430
display id: range2066430
version: 1
direction: direction/reverse
top level: BBa_J18934
start: 453
end: 680
orientation: inline

BBa_J18909
type: SequenceAnnotation
persistent identity: annotation2066437
display id: annotation2066437
version: 1
top level: BBa_J18934
location: https://synbiohub.org/public/igem/BBa_J18934/annotation2066437/range2066437/1
component: BBa_J18909

https://synbiohub.org/public/igem/BBa_J18934/annotation2066437/range2066437/1
type: Range
persistent identity: range2066437
display id: range2066437
version: 1
direction: direction/reverse
top level: BBa_J18934
start: 717
end: 734
orientation: inline

BBa_K801043
type: ComponentDefinition
persistent identity: BBa K801043
display id: BBa_K801043
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LexA based yeast light-swithable promoter system
creator: Dong-Jiunn Jeffery TRUONG
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1057_
m user id: 0
owner id: 11507
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: composite part of Bba_K319003, K801039, BBa_K801011 , Bba_K319003, Bba_K801041, and BBa_K801011
mutable notes: -
mutable provenance: composite part of Bba_K319003, K801039, BBa_K801011 , Bba_K319003, Bba_K801041, and BBa_K801011
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: BBa_K319003 (pTEF1, TEF1 promoter)
sequence annotation: T7 promoter (for sequencing)
sequence annotation: HA epitope tage
sequence annotation: BBa_K365005 (20 amino acids long linker)
sequence annotation: BBa_K801041 (SV40NLS-PhyB-Linker-LexA)
sequence annotation: BBa_K801011, (tTEF1, TEF1 terminator)
sequence annotation: Phytochrome B (PhyB, 908 first N-terminal amino acids)
sequence annotation: BBa_K801039 (SV40NLS-GAL4AD-Linker-PIF3)
sequence annotation: GAL4AD (GAL4 transcriptonal activation domain)
sequence annotation: PIF3 (First 100 N-terminal amino acids)
sequence annotation: SV40 NLS (SV40 nuclear localization signal)
sequence annotation: SV40NLS-PhyB-Linker-LexA
sequence annotation: Linker
sequence annotation: LexA
sequence: https://synbiohub.org/public/igem/BBa_K801043_sequence/1

BBa_K319003 (pTEF1, TEF1 promoter)
type: SequenceAnnotation
persistent identity: annotation2198806
display id: annotation2198806
version: 1
top level: BBa_K801043
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K801043/annotation2198806/range2198806/1

https://synbiohub.org/public/igem/BBa_K801043/annotation2198806/range2198806/1
type: Range
persistent identity: range2198806
display id: range2198806
version: 1
direction: direction/forward
top level: BBa_K801043
start: 1782
end: 2231
orientation: inline

T7 promoter (for sequencing)
type: SequenceAnnotation
persistent identity: annotation2198802
display id: annotation2198802
version: 1
top level: BBa_K801043
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K801043/annotation2198802/range2198802/1

https://synbiohub.org/public/igem/BBa_K801043/annotation2198802/range2198802/1
type: Range
persistent identity: range2198802
display id: range2198802
version: 1
direction: direction/forward
top level: BBa_K801043
start: 869
end: 891
orientation: inline

HA epitope tage
type: SequenceAnnotation
persistent identity: annotation2198803
display id: annotation2198803
version: 1
top level: BBa_K801043
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801043/annotation2198803/range2198803/1

https://synbiohub.org/public/igem/BBa_K801043/annotation2198803/range2198803/1
type: Range
persistent identity: range2198803
display id: range2198803
version: 1
direction: direction/forward
top level: BBa_K801043
start: 912
end: 932
orientation: inline

BBa_K365005 (20 amino acids long linker)
type: SequenceAnnotation
persistent identity: annotation2198810
display id: annotation2198810
version: 1
top level: BBa_K801043
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801043/annotation2198810/range2198810/1

https://synbiohub.org/public/igem/BBa_K801043/annotation2198810/range2198810/1
type: Range
persistent identity: range2198810
display id: range2198810
version: 1
direction: direction/forward
top level: BBa_K801043
start: 5036
end: 5095
orientation: inline

BBa_K801041 (SV40NLS-PhyB-Linker-LexA)
type: SequenceAnnotation
persistent identity: annotation2198814
display id: annotation2198814
version: 1
top level: BBa_K801043
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801043/annotation2198814/range2198814/1

https://synbiohub.org/public/igem/BBa_K801043/annotation2198814/range2198814/1
type: Range
persistent identity: range2198814
display id: range2198814
version: 1
direction: direction/forward
top level: BBa_K801043
start: 2246
end: 5704
orientation: inline

BBa_K801011, (tTEF1, TEF1 terminator)
type: SequenceAnnotation
persistent identity: annotation2198812
display id: annotation2198812
version: 1
top level: BBa_K801043
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801043/annotation2198812/range2198812/1

https://synbiohub.org/public/igem/BBa_K801043/annotation2198812/range2198812/1
type: Range
persistent identity: range2198812
display id: range2198812
version: 1
direction: direction/forward
top level: BBa_K801043
start: 5722
end: 6228
orientation: inline

Phytochrome B (PhyB, 908 first N-terminal amino acids)
type: SequenceAnnotation
persistent identity: annotation2198809
display id: annotation2198809
version: 1
top level: BBa_K801043
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K801043/annotation2198809/range2198809/1

https://synbiohub.org/public/igem/BBa_K801043/annotation2198809/range2198809/1
type: Range
persistent identity: range2198809
display id: range2198809
version: 1
direction: direction/forward
top level: BBa_K801043
start: 2309
end: 5029
orientation: inline

BBa_K801039 (SV40NLS-GAL4AD-Linker-PIF3)
type: SequenceAnnotation
persistent identity: annotation2198798
display id: annotation2198798
version: 1
top level: BBa_K801043
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801043/annotation2198798/range2198798/1

https://synbiohub.org/public/igem/BBa_K801043/annotation2198798/range2198798/1
type: Range
persistent identity: range2198798
display id: range2198798
version: 1
direction: direction/forward
top level: BBa_K801043
start: 456
end: 1259
orientation: inline

GAL4AD (GAL4 transcriptonal activation domain)
type: SequenceAnnotation
persistent identity: annotation2198800
display id: annotation2198800
version: 1
top level: BBa_K801043
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K801043/annotation2198800/range2198800/1

https://synbiohub.org/public/igem/BBa_K801043/annotation2198800/range2198800/1
type: Range
persistent identity: range2198800
display id: range2198800
version: 1
direction: direction/forward
top level: BBa_K801043
start: 525
end: 863
orientation: inline

PIF3 (First 100 N-terminal amino acids)
type: SequenceAnnotation
persistent identity: annotation2198804
display id: annotation2198804
version: 1
top level: BBa_K801043
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K801043/annotation2198804/range2198804/1

https://synbiohub.org/public/igem/BBa_K801043/annotation2198804/range2198804/1
type: Range
persistent identity: range2198804
display id: range2198804
version: 1
direction: direction/forward
top level: BBa_K801043
start: 960
end: 1256
orientation: inline

BBa_K319003 (pTEF1, TEF1 promoter)
type: SequenceAnnotation
persistent identity: annotation2198797
display id: annotation2198797
version: 1
top level: BBa_K801043
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K801043/annotation2198797/range2198797/1

https://synbiohub.org/public/igem/BBa_K801043/annotation2198797/range2198797/1
type: Range
persistent identity: range2198797
display id: range2198797
version: 1
direction: direction/forward
top level: BBa_K801043
start: 1
end: 450
orientation: inline

SV40 NLS (SV40 nuclear localization signal)
type: SequenceAnnotation
persistent identity: annotation2198808
display id: annotation2198808
version: 1
top level: BBa_K801043
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801043/annotation2198808/range2198808/1

https://synbiohub.org/public/igem/BBa_K801043/annotation2198808/range2198808/1
type: Range
persistent identity: range2198808
display id: range2198808
version: 1
direction: direction/forward
top level: BBa_K801043
start: 2246
end: 2302
orientation: inline

SV40NLS-PhyB-Linker-LexA
type: SequenceAnnotation
persistent identity: annotation2198807
display id: annotation2198807
version: 1
top level: BBa_K801043
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801043/annotation2198807/range2198807/1

https://synbiohub.org/public/igem/BBa_K801043/annotation2198807/range2198807/1
type: Range
persistent identity: range2198807
display id: range2198807
version: 1
direction: direction/forward
top level: BBa_K801043
start: 2237
end: 5713
orientation: inline

SV40 NLS (SV40 nuclear localization signal)
type: SequenceAnnotation
persistent identity: annotation2198799
display id: annotation2198799
version: 1
top level: BBa_K801043
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801043/annotation2198799/range2198799/1

https://synbiohub.org/public/igem/BBa_K801043/annotation2198799/range2198799/1
type: Range
persistent identity: range2198799
display id: range2198799
version: 1
direction: direction/forward
top level: BBa_K801043
start: 465
end: 521
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2198801
display id: annotation2198801
version: 1
top level: BBa_K801043
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801043/annotation2198801/range2198801/1

https://synbiohub.org/public/igem/BBa_K801043/annotation2198801/range2198801/1
type: Range
persistent identity: range2198801
display id: range2198801
version: 1
direction: direction/forward
top level: BBa_K801043
start: 864
end: 959
orientation: inline

LexA
type: SequenceAnnotation
persistent identity: annotation2198811
display id: annotation2198811
version: 1
top level: BBa_K801043
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K801043/annotation2198811/range2198811/1

https://synbiohub.org/public/igem/BBa_K801043/annotation2198811/range2198811/1
type: Range
persistent identity: range2198811
display id: range2198811
version: 1
direction: direction/forward
top level: BBa_K801043
start: 5102
end: 5704
orientation: inline

BBa_K801011, (tTEF1, TEF1 terminator)
type: SequenceAnnotation
persistent identity: annotation2198805
display id: annotation2198805
version: 1
top level: BBa_K801043
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801043/annotation2198805/range2198805/1

https://synbiohub.org/public/igem/BBa_K801043/annotation2198805/range2198805/1
type: Range
persistent identity: range2198805
display id: range2198805
version: 1
direction: direction/forward
top level: BBa_K801043
start: 1268
end: 1774
orientation: inline

BBa_J18940
type: ComponentDefinition
persistent identity: BBa J18940
display id: BBa_J18940
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FRB ~ GLucFrag1
creator: Raik Gruenberg
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
discontinued: false
dominant: false
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: P08 expression cassette from Gruenberg et al. 2010 series of interaction reporter proteins. All proteins share the same architecture: * T7Start-domain1-5xGS-domain2-preSCsite-HisTag-T7Stop Only Domains1 and 2 vary and are taken from a list of protein interaction input and output (readout) parts. ===Expression=== Proteins were expressed in E. coli BL21 using T7 Polymerase and were purified by His-tag affinity. ===Reference=== * Gruenberg, Ferrar, van der Sloot, Serrano (2010): Building Blocks for Protein Interaction Devices
mutable notes: Constructs were assembled according to R.F.C. 25. All parts were codon optimized for E. coli.
mutable provenance: synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0105
component: BBa_J18909
component: BBa_J18926
component: BBa_J18912
component: BBa_J18913
component: BBa_J18922
component: BBa_J18928
component: BBa_J18919
sequence annotation: BBa_B0105
sequence annotation: BBa_J18928
sequence annotation: BBa_J18926
sequence annotation: BBa_J18922
sequence annotation: BBa_J18912
sequence annotation: BBa_J18909
sequence annotation: BBa_J18913
sequence annotation: BBa_J18919
sequence: https://synbiohub.org/public/igem/BBa_J18940_sequence/1

BBa_B0105
type: Component
persistent identity: component2066548
display id: component2066548
version: 1
top level: BBa_J18940
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_J18909
type: Component
persistent identity: component2066553
display id: component2066553
version: 1
top level: BBa_J18940
access: public
definition: https://synbiohub.org/public/igem/BBa_J18909/1

BBa_J18926
type: Component
persistent identity: component2066540
display id: component2066540
version: 1
top level: BBa_J18940
access: public
definition: FRB

BBa_B0105
type: Component
persistent identity: component2066545
display id: component2066545
version: 1
top level: BBa_J18940
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_B0105
type: Component
persistent identity: component2066555
display id: component2066555
version: 1
top level: BBa_J18940
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_B0105
type: Component
persistent identity: component2066542
display id: component2066542
version: 1
top level: BBa_J18940
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_B0105
type: Component
persistent identity: component2066551
display id: component2066551
version: 1
top level: BBa_J18940
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_J18912
type: Component
persistent identity: component2066537
display id: component2066537
version: 1
top level: BBa_J18940
access: public
definition: https://synbiohub.org/public/igem/BBa_J18912/1

BBa_J18913
type: Component
persistent identity: component2066557
display id: component2066557
version: 1
top level: BBa_J18940
access: public
definition: https://synbiohub.org/public/igem/BBa_J18913/1

BBa_J18922
type: Component
persistent identity: component2066543
display id: component2066543
version: 1
top level: BBa_J18940
access: public
definition: https://synbiohub.org/public/igem/BBa_J18922/1

BBa_J18928
type: Component
persistent identity: component2066546
display id: component2066546
version: 1
top level: BBa_J18940
access: public
definition: GLucFrag1

BBa_J18919
type: Component
persistent identity: component2066549
display id: component2066549
version: 1
top level: BBa_J18940
access: public
definition: https://synbiohub.org/public/igem/BBa_J18919/1

BBa_B0105
type: Component
persistent identity: component2066539
display id: component2066539
version: 1
top level: BBa_J18940
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066551
display id: annotation2066551
version: 1
top level: BBa_J18940
location: https://synbiohub.org/public/igem/BBa_J18940/annotation2066551/range2066551/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18940/annotation2066551/range2066551/1
type: Range
persistent identity: range2066551
display id: range2066551
version: 1
direction: direction/reverse
top level: BBa_J18940
start: 717
end: 722
orientation: inline

BBa_J18928
type: SequenceAnnotation
persistent identity: annotation2066546
display id: annotation2066546
version: 1
top level: BBa_J18940
location: https://synbiohub.org/public/igem/BBa_J18940/annotation2066546/range2066546/1
component: BBa_J18928

https://synbiohub.org/public/igem/BBa_J18940/annotation2066546/range2066546/1
type: Range
persistent identity: range2066546
display id: range2066546
version: 1
direction: direction/reverse
top level: BBa_J18940
start: 411
end: 686
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066545
display id: annotation2066545
version: 1
top level: BBa_J18940
location: https://synbiohub.org/public/igem/BBa_J18940/annotation2066545/range2066545/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18940/annotation2066545/range2066545/1
type: Range
persistent identity: range2066545
display id: range2066545
version: 1
direction: direction/reverse
top level: BBa_J18940
start: 405
end: 410
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066548
display id: annotation2066548
version: 1
top level: BBa_J18940
location: https://synbiohub.org/public/igem/BBa_J18940/annotation2066548/range2066548/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18940/annotation2066548/range2066548/1
type: Range
persistent identity: range2066548
display id: range2066548
version: 1
direction: direction/reverse
top level: BBa_J18940
start: 687
end: 692
orientation: inline

BBa_J18926
type: SequenceAnnotation
persistent identity: annotation2066540
display id: annotation2066540
version: 1
top level: BBa_J18940
location: https://synbiohub.org/public/igem/BBa_J18940/annotation2066540/range2066540/1
component: BBa_J18926

https://synbiohub.org/public/igem/BBa_J18940/annotation2066540/range2066540/1
type: Range
persistent identity: range2066540
display id: range2066540
version: 1
direction: direction/reverse
top level: BBa_J18940
start: 90
end: 368
orientation: inline

BBa_J18922
type: SequenceAnnotation
persistent identity: annotation2066543
display id: annotation2066543
version: 1
top level: BBa_J18940
location: https://synbiohub.org/public/igem/BBa_J18940/annotation2066543/range2066543/1
component: BBa_J18922

https://synbiohub.org/public/igem/BBa_J18940/annotation2066543/range2066543/1
type: Range
persistent identity: range2066543
display id: range2066543
version: 1
direction: direction/reverse
top level: BBa_J18940
start: 375
end: 404
orientation: inline

BBa_J18912
type: SequenceAnnotation
persistent identity: annotation2066537
display id: annotation2066537
version: 1
top level: BBa_J18940
location: https://synbiohub.org/public/igem/BBa_J18940/annotation2066537/range2066537/1
component: BBa_J18912

https://synbiohub.org/public/igem/BBa_J18940/annotation2066537/range2066537/1
type: Range
persistent identity: range2066537
display id: range2066537
version: 1
direction: direction/reverse
top level: BBa_J18940
start: 1
end: 83
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066539
display id: annotation2066539
version: 1
top level: BBa_J18940
location: https://synbiohub.org/public/igem/BBa_J18940/annotation2066539/range2066539/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18940/annotation2066539/range2066539/1
type: Range
persistent identity: range2066539
display id: range2066539
version: 1
direction: direction/reverse
top level: BBa_J18940
start: 84
end: 89
orientation: inline

BBa_J18909
type: SequenceAnnotation
persistent identity: annotation2066553
display id: annotation2066553
version: 1
top level: BBa_J18940
location: https://synbiohub.org/public/igem/BBa_J18940/annotation2066553/range2066553/1
component: BBa_J18909

https://synbiohub.org/public/igem/BBa_J18940/annotation2066553/range2066553/1
type: Range
persistent identity: range2066553
display id: range2066553
version: 1
direction: direction/reverse
top level: BBa_J18940
start: 723
end: 740
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066542
display id: annotation2066542
version: 1
top level: BBa_J18940
location: https://synbiohub.org/public/igem/BBa_J18940/annotation2066542/range2066542/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18940/annotation2066542/range2066542/1
type: Range
persistent identity: range2066542
display id: range2066542
version: 1
direction: direction/reverse
top level: BBa_J18940
start: 369
end: 374
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066555
display id: annotation2066555
version: 1
top level: BBa_J18940
location: https://synbiohub.org/public/igem/BBa_J18940/annotation2066555/range2066555/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18940/annotation2066555/range2066555/1
type: Range
persistent identity: range2066555
display id: range2066555
version: 1
direction: direction/reverse
top level: BBa_J18940
start: 741
end: 746
orientation: inline

BBa_J18913
type: SequenceAnnotation
persistent identity: annotation2066557
display id: annotation2066557
version: 1
top level: BBa_J18940
location: https://synbiohub.org/public/igem/BBa_J18940/annotation2066557/range2066557/1
component: BBa_J18913

https://synbiohub.org/public/igem/BBa_J18940/annotation2066557/range2066557/1
type: Range
persistent identity: range2066557
display id: range2066557
version: 1
direction: direction/reverse
top level: BBa_J18940
start: 747
end: 881
orientation: inline

BBa_J18919
type: SequenceAnnotation
persistent identity: annotation2066549
display id: annotation2066549
version: 1
top level: BBa_J18940
location: https://synbiohub.org/public/igem/BBa_J18940/annotation2066549/range2066549/1
component: BBa_J18919

https://synbiohub.org/public/igem/BBa_J18940/annotation2066549/range2066549/1
type: Range
persistent identity: range2066549
display id: range2066549
version: 1
direction: direction/reverse
top level: BBa_J18940
start: 693
end: 716
orientation: inline

CBDclos(T7
type: ComponentDefinition
persistent identity: BBa K863112
display id: BBa_K863112
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cellulose binding domain of C. cellulovorans with T7, RBS, GS-Linker (Freiburg-Standard)
creator: Moritz M??ller
created: 2012-09-19T11:00:00Z
modified: 2015-06-17T01:20:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1123_
m user id: 4206
owner id: 13080
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Cellulose binding domain of the [http://www.ncbi.nlm.nih.gov/nucleotide/327179207?report=genbank&log$=nucltop&blast_rank=3&RID=152ZCN0E01N Cellulomonas fimi ATCC 484 exoglucanase gene] in Freiburg-standard
mutable notes: Added the regulatory unit BBa_K525998 (T7+RBS) in front of the CBD and a short GS-Linker behind the domain and used Freiburg-Standard to be able to fuse proteins to the domain.
mutable provenance: Cloning-vector
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: BBa_K525998
sequence annotation: TAA
sequence annotation: B0034
sequence annotation: Cellulose Binding Protein
sequence annotation: silent mutation (A original)
sequence annotation: AgeI
sequence annotation: RBS
sequence annotation: BBa_K863111
sequence annotation: GS-Linker
sequence annotation: ATG
sequence annotation: Cellulose binding domain (CBDclos)
sequence annotation: T7 promoter
sequence: https://synbiohub.org/public/igem/BBa_K863112_sequence/1

BBa_K525998
type: SequenceAnnotation
persistent identity: annotation2188283
display id: annotation2188283
version: 1
top level: CBDclos(T7
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863112/annotation2188283/range2188283/1

https://synbiohub.org/public/igem/BBa_K863112/annotation2188283/range2188283/1
type: Range
persistent identity: range2188283
display id: range2188283
version: 1
direction: direction/forward
top level: CBDclos(T7
start: 1
end: 32
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2188293
display id: annotation2188293
version: 1
top level: CBDclos(T7
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863112/annotation2188293/range2188293/1

https://synbiohub.org/public/igem/BBa_K863112/annotation2188293/range2188293/1
type: Range
persistent identity: range2188293
display id: range2188293
version: 1
direction: direction/forward
top level: CBDclos(T7
start: 335
end: 337
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2188284
display id: annotation2188284
version: 1
top level: CBDclos(T7
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863112/annotation2188284/range2188284/1

https://synbiohub.org/public/igem/BBa_K863112/annotation2188284/range2188284/1
type: Range
persistent identity: range2188284
display id: range2188284
version: 1
direction: direction/forward
top level: CBDclos(T7
start: 21
end: 32
orientation: inline

Cellulose Binding Protein
type: SequenceAnnotation
persistent identity: annotation2188290
display id: annotation2188290
version: 1
top level: CBDclos(T7
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863112/annotation2188290/range2188290/1

https://synbiohub.org/public/igem/BBa_K863112/annotation2188290/range2188290/1
type: Range
persistent identity: range2188290
display id: range2188290
version: 1
direction: direction/forward
top level: CBDclos(T7
start: 317
end: 322
orientation: inline

silent mutation (A original)
type: SequenceAnnotation
persistent identity: annotation2200498
display id: annotation2200498
version: 1
top level: CBDclos(T7
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K863112/annotation2200498/range2200498/1

https://synbiohub.org/public/igem/BBa_K863112/annotation2200498/range2200498/1
type: Range
persistent identity: range2200498
display id: range2200498
version: 1
direction: direction/forward
top level: CBDclos(T7
start: 112
end: 112
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2188292
display id: annotation2188292
version: 1
top level: CBDclos(T7
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863112/annotation2188292/range2188292/1

https://synbiohub.org/public/igem/BBa_K863112/annotation2188292/range2188292/1
type: Range
persistent identity: range2188292
display id: range2188292
version: 1
direction: direction/forward
top level: CBDclos(T7
start: 329
end: 334
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2188285
display id: annotation2188285
version: 1
top level: CBDclos(T7
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K863112/annotation2188285/range2188285/1

https://synbiohub.org/public/igem/BBa_K863112/annotation2188285/range2188285/1
type: Range
persistent identity: range2188285
display id: range2188285
version: 1
direction: direction/forward
top level: CBDclos(T7
start: 24
end: 27
orientation: inline

BBa_K863111
type: SequenceAnnotation
persistent identity: annotation2188286
display id: annotation2188286
version: 1
top level: CBDclos(T7
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863112/annotation2188286/range2188286/1

https://synbiohub.org/public/igem/BBa_K863112/annotation2188286/range2188286/1
type: Range
persistent identity: range2188286
display id: range2188286
version: 1
direction: direction/forward
top level: CBDclos(T7
start: 35
end: 337
orientation: inline

GS-Linker
type: SequenceAnnotation
persistent identity: annotation2188291
display id: annotation2188291
version: 1
top level: CBDclos(T7
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863112/annotation2188291/range2188291/1

https://synbiohub.org/public/igem/BBa_K863112/annotation2188291/range2188291/1
type: Range
persistent identity: range2188291
display id: range2188291
version: 1
direction: direction/forward
top level: CBDclos(T7
start: 323
end: 328
orientation: inline

Cellulose Binding Protein
type: SequenceAnnotation
persistent identity: annotation2188288
display id: annotation2188288
version: 1
top level: CBDclos(T7
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863112/annotation2188288/range2188288/1

https://synbiohub.org/public/igem/BBa_K863112/annotation2188288/range2188288/1
type: Range
persistent identity: range2188288
display id: range2188288
version: 1
direction: direction/forward
top level: CBDclos(T7
start: 35
end: 40
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2188287
display id: annotation2188287
version: 1
top level: CBDclos(T7
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863112/annotation2188287/range2188287/1

https://synbiohub.org/public/igem/BBa_K863112/annotation2188287/range2188287/1
type: Range
persistent identity: range2188287
display id: range2188287
version: 1
direction: direction/forward
top level: CBDclos(T7
start: 35
end: 38
orientation: inline

Cellulose binding domain (CBDclos)
type: SequenceAnnotation
persistent identity: annotation2188289
display id: annotation2188289
version: 1
top level: CBDclos(T7
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K863112/annotation2188289/range2188289/1

https://synbiohub.org/public/igem/BBa_K863112/annotation2188289/range2188289/1
type: Range
persistent identity: range2188289
display id: range2188289
version: 1
direction: direction/forward
top level: CBDclos(T7
start: 41
end: 316
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2188282
display id: annotation2188282
version: 1
top level: CBDclos(T7
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K863112/annotation2188282/range2188282/1

https://synbiohub.org/public/igem/BBa_K863112/annotation2188282/range2188282/1
type: Range
persistent identity: range2188282
display id: range2188282
version: 1
direction: direction/forward
top level: CBDclos(T7
start: 1
end: 20
orientation: inline

occupyDNAP
type: ComponentDefinition
persistent identity: BBa M36549
display id: BBa_M36549
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PoPS --> N4 gp8 (DNA replication inhibitor)
creator: Rashmi Sharma, Bryce Bajar, Kendrick Wang
created: 2011-12-08T12:00:00Z
modified: 2015-05-08T01:14:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _848_
m user id: 0
owner id: 11054
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: to be added
mutable notes: to be added
mutable provenance: to be added
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_M36292
component: BBa_B0032
component: BBa_M36548
sequence annotation: BBa_B0032
sequence annotation: BBa_M36292
sequence annotation: BBa_M36548
sequence: https://synbiohub.org/public/igem/BBa_M36549_sequence/1

BBa_M36292
type: Component
persistent identity: component2166552
display id: component2166552
version: 1
top level: occupyDNAP
access: public
definition: https://synbiohub.org/public/igem/BBa_M36292/1

BBa_B0032
type: Component
persistent identity: component2166548
display id: component2166548
version: 1
top level: occupyDNAP
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_M36548
type: Component
persistent identity: component2166551
display id: component2166551
version: 1
top level: occupyDNAP
access: public
definition: N4 gp8

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2166548
display id: annotation2166548
version: 1
top level: occupyDNAP
location: https://synbiohub.org/public/igem/BBa_M36549/annotation2166548/range2166548/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_M36549/annotation2166548/range2166548/1
type: Range
persistent identity: range2166548
display id: range2166548
version: 1
direction: direction/forward
top level: occupyDNAP
start: 1
end: 13
orientation: inline

BBa_M36292
type: SequenceAnnotation
persistent identity: annotation2166552
display id: annotation2166552
version: 1
top level: occupyDNAP
location: https://synbiohub.org/public/igem/BBa_M36549/annotation2166552/range2166552/1
component: BBa_M36292

https://synbiohub.org/public/igem/BBa_M36549/annotation2166552/range2166552/1
type: Range
persistent identity: range2166552
display id: range2166552
version: 1
direction: direction/reverse
top level: occupyDNAP
start: 244
end: 325
orientation: inline

BBa_M36548
type: SequenceAnnotation
persistent identity: annotation2166551
display id: annotation2166551
version: 1
top level: occupyDNAP
location: https://synbiohub.org/public/igem/BBa_M36549/annotation2166551/range2166551/1
component: BBa_M36548

https://synbiohub.org/public/igem/BBa_M36549/annotation2166551/range2166551/1
type: Range
persistent identity: range2166551
display id: range2166551
version: 1
direction: direction/reverse
top level: occupyDNAP
start: 20
end: 235
orientation: inline

BBa_K352001
type: ComponentDefinition
persistent identity: BBa K352001
display id: BBa_K352001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CooA from Rhodospirillum rubrum
creator: Cihan Tastan
created: 2010-10-01T11:00:00Z
modified: 2015-05-08T01:12:11Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _474_606_
m user id: 0
owner id: 5815
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: CooA is a heme-containing transcriptional activator that enables Rhodospirillum rubrum to sense and grow on CO as a sole energy source. CooA is a member of a family of transcriptional regulators similar to the cAMP receptor protein and fumavate nitrate reduction from Escherichia coli. The protein is active in sequence-specific DNA binding in the presence of CO, but not in the absence of CO. The protein to be a dimer in the absence of CO. The product, CooA, is 28% identical (51% similar) to CRP(cAMP receptor protein) and 18% identical (45% similar) to FNR(fumavate nitrate reduction) from Escherichia coli. Inactive Fe(II) CooA structure adapted from that of the strain with PDB identification no. 1FT9. The protein consists of two monomers, shaded differently, which dimerize along the central C-helices of adjacent effector-binding domains. The solved structure is asymmetric, in which one monomer contains fused C- and D-helices. Nonetheless, both F-helices that interact with DNA in a sequence-specific manner are buried from the surface in the structure. The 4/5 loop is noted and so are the Pro2 and His77 heme Fe(II) ligands.
mutable notes: The sequence information was acquired from NCBI and physical DNA was synthesized from GENEART. Classical cloning strategies(restriction digestion) were used to produce biobricks.
mutable provenance: Hwan Youn, Robert L. Kerby, Mary Conrad, et al. 2004. Functionally Critical Elements of CooA-Related CO Sensors. J. Bacteriol. 186(5):1320-1329. doi:10.1128/JB.186.5.1320-1329.2004.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Start Codon
sequence: https://synbiohub.org/public/igem/BBa_K352001_sequence/1

Start Codon
type: SequenceAnnotation
persistent identity: annotation2081251
display id: annotation2081251
version: 1
top level: BBa_K352001
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K352001/annotation2081251/range2081251/1

https://synbiohub.org/public/igem/BBa_K352001/annotation2081251/range2081251/1
type: Range
persistent identity: range2081251
display id: range2081251
version: 1
direction: direction/forward
top level: BBa_K352001
start: 1
end: 3
orientation: inline

hPCD
type: ComponentDefinition
persistent identity: BBa J176000
display id: BBa_J176000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: hPCD
creator: Karmella Haynes
created: 2011-06-23T11:00:00Z
modified: 2015-08-31T04:08:33Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _863_
m user id: 0
owner id: 1144
owning group id: 61
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Human Polycomb chromodomain from the CBX8 protein (a.a. 1-63). See Haynes and Silver, JBC, 2011
mutable notes: The sequence was not modified.
mutable provenance: Synthesized by GenScript.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_J176000_sequence/1

CBDcex(T7)
type: ComponentDefinition
persistent identity: BBa K863103
display id: BBa_K863103
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cellulose binding Domain from Cellulomonas Fimi with Reporter GFP
creator: Moritz M??ller
created: 2012-09-19T11:00:00Z
modified: 2015-06-17T01:19:26Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1123_
m user id: 4206
owner id: 13080
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Cellulose binding Domain of C. Fimi Exoglucanase gene with T7, RBS linked with two amino acids (GS) to a GFP (Freiburg-Standard)
mutable notes: CBDcex fused to a his-tagged GFP in a Freiburg-manner
mutable provenance: Cloning-vector and [http://partsregistry.org/Part:BBa_K863121 BBa_K863121]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: T7 promoter
sequence annotation: B0034
sequence annotation: GS-Linker
sequence annotation: Exoglucanase
sequence annotation: BBa_K863101
sequence annotation: BBa_K863102
sequence annotation: BBa_E0040
sequence annotation: RBS
sequence annotation: AgeI
sequence annotation: ATG
sequence annotation: BBa_K525998
sequence annotation: TAA
sequence annotation: His-Tag
sequence annotation: Freiburg-Scar
sequence annotation: Cellulose binding domain (CBDcex)
sequence annotation: BBa_K863121
sequence: https://synbiohub.org/public/igem/BBa_K863103_sequence/1

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2188012
display id: annotation2188012
version: 1
top level: CBDcex(T7)
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188012/range2188012/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188012/range2188012/1
type: Range
persistent identity: range2188012
display id: range2188012
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 1
end: 20
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2188015
display id: annotation2188015
version: 1
top level: CBDcex(T7)
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188015/range2188015/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188015/range2188015/1
type: Range
persistent identity: range2188015
display id: range2188015
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 21
end: 32
orientation: inline

GS-Linker
type: SequenceAnnotation
persistent identity: annotation2188022
display id: annotation2188022
version: 1
top level: CBDcex(T7)
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188022/range2188022/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188022/range2188022/1
type: Range
persistent identity: range2188022
display id: range2188022
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 359
end: 364
orientation: inline

Exoglucanase
type: SequenceAnnotation
persistent identity: annotation2188018
display id: annotation2188018
version: 1
top level: CBDcex(T7)
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188018/range2188018/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188018/range2188018/1
type: Range
persistent identity: range2188018
display id: range2188018
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 38
end: 49
orientation: inline

BBa_K863101
type: SequenceAnnotation
persistent identity: annotation2188019
display id: annotation2188019
version: 1
top level: CBDcex(T7)
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188019/range2188019/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188019/range2188019/1
type: Range
persistent identity: range2188019
display id: range2188019
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 38
end: 364
orientation: inline

BBa_K863102
type: SequenceAnnotation
persistent identity: annotation2188014
display id: annotation2188014
version: 1
top level: CBDcex(T7)
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188014/range2188014/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188014/range2188014/1
type: Range
persistent identity: range2188014
display id: range2188014
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 1
end: 364
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2188024
display id: annotation2188024
version: 1
top level: CBDcex(T7)
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188024/range2188024/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188024/range2188024/1
type: Range
persistent identity: range2188024
display id: range2188024
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 371
end: 1084
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2188016
display id: annotation2188016
version: 1
top level: CBDcex(T7)
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188016/range2188016/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188016/range2188016/1
type: Range
persistent identity: range2188016
display id: range2188016
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 24
end: 27
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2188027
display id: annotation2188027
version: 1
top level: CBDcex(T7)
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188027/range2188027/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188027/range2188027/1
type: Range
persistent identity: range2188027
display id: range2188027
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 1103
end: 1108
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2188017
display id: annotation2188017
version: 1
top level: CBDcex(T7)
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188017/range2188017/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188017/range2188017/1
type: Range
persistent identity: range2188017
display id: range2188017
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 35
end: 37
orientation: inline

Exoglucanase
type: SequenceAnnotation
persistent identity: annotation2188021
display id: annotation2188021
version: 1
top level: CBDcex(T7)
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188021/range2188021/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188021/range2188021/1
type: Range
persistent identity: range2188021
display id: range2188021
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 350
end: 358
orientation: inline

BBa_K525998
type: SequenceAnnotation
persistent identity: annotation2188013
display id: annotation2188013
version: 1
top level: CBDcex(T7)
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188013/range2188013/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188013/range2188013/1
type: Range
persistent identity: range2188013
display id: range2188013
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 1
end: 32
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2188028
display id: annotation2188028
version: 1
top level: CBDcex(T7)
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188028/range2188028/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188028/range2188028/1
type: Range
persistent identity: range2188028
display id: range2188028
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 1109
end: 1111
orientation: inline

His-Tag
type: SequenceAnnotation
persistent identity: annotation2188026
display id: annotation2188026
version: 1
top level: CBDcex(T7)
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188026/range2188026/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188026/range2188026/1
type: Range
persistent identity: range2188026
display id: range2188026
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 1085
end: 1102
orientation: inline

Freiburg-Scar
type: SequenceAnnotation
persistent identity: annotation2188023
display id: annotation2188023
version: 1
top level: CBDcex(T7)
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188023/range2188023/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188023/range2188023/1
type: Range
persistent identity: range2188023
display id: range2188023
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 365
end: 370
orientation: inline

Cellulose binding domain (CBDcex)
type: SequenceAnnotation
persistent identity: annotation2188020
display id: annotation2188020
version: 1
top level: CBDcex(T7)
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188020/range2188020/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188020/range2188020/1
type: Range
persistent identity: range2188020
display id: range2188020
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 50
end: 349
orientation: inline

BBa_K863121
type: SequenceAnnotation
persistent identity: annotation2188025
display id: annotation2188025
version: 1
top level: CBDcex(T7)
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188025/range2188025/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188025/range2188025/1
type: Range
persistent identity: range2188025
display id: range2188025
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 371
end: 1111
orientation: inline

CBDclos
type: ComponentDefinition
persistent identity: BBa K863111
display id: BBa_K863111
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cellulose binding domain of C. cellulovorans cellulose binding protein gene (Freiburg-Standard)
creator: Moritz M??ller
created: 2012-09-17T11:00:00Z
modified: 2015-06-17T01:18:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1123_
m user id: 4206
owner id: 13080
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Cellulose binding Domain of Clostridium cellulovorans cellulose binding protein gene (cbp A) http://www.ncbi.nlm.nih.gov/nuccore/M73817
mutable notes: The sequence starts with the Freiburg-prefix (ATG+NgoMIV-restriction-site) followed by 12 Bases (4 AS) of the cellulose binding protein gene in front of the CBD; then 12 bases (4 amino acids) of the cellulose binding protein gene behind the CBD; after that the sequence continues with a short GS-Linker and ends with the Freiburg-suffix (AgeI-restirction-site+TAA).
mutable provenance: Cloning-Vector
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: AgeI
sequence annotation: TAA
sequence annotation: ATG
sequence annotation: Cellulose Binding Protein
sequence annotation: Siltent mutation (A original)
sequence annotation: NgoMIV
sequence annotation: Cellulose binding domain (CBDclos)
sequence annotation: GS-Linker
sequence: https://synbiohub.org/public/igem/BBa_K863111_sequence/1

AgeI
type: SequenceAnnotation
persistent identity: annotation2185123
display id: annotation2185123
version: 1
top level: CBDclos
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863111/annotation2185123/range2185123/1

https://synbiohub.org/public/igem/BBa_K863111/annotation2185123/range2185123/1
type: Range
persistent identity: range2185123
display id: range2185123
version: 1
direction: direction/forward
top level: CBDclos
start: 310
end: 315
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2185122
display id: annotation2185122
version: 1
top level: CBDclos
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863111/annotation2185122/range2185122/1

https://synbiohub.org/public/igem/BBa_K863111/annotation2185122/range2185122/1
type: Range
persistent identity: range2185122
display id: range2185122
version: 1
direction: direction/forward
top level: CBDclos
start: 316
end: 318
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2185119
display id: annotation2185119
version: 1
top level: CBDclos
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863111/annotation2185119/range2185119/1

https://synbiohub.org/public/igem/BBa_K863111/annotation2185119/range2185119/1
type: Range
persistent identity: range2185119
display id: range2185119
version: 1
direction: direction/forward
top level: CBDclos
start: 1
end: 3
orientation: inline

Cellulose Binding Protein
type: SequenceAnnotation
persistent identity: annotation2185121
display id: annotation2185121
version: 1
top level: CBDclos
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863111/annotation2185121/range2185121/1

https://synbiohub.org/public/igem/BBa_K863111/annotation2185121/range2185121/1
type: Range
persistent identity: range2185121
display id: range2185121
version: 1
direction: direction/forward
top level: CBDclos
start: 10
end: 21
orientation: inline

Siltent mutation (A original)
type: SequenceAnnotation
persistent identity: annotation2202131
display id: annotation2202131
version: 1
top level: CBDclos
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K863111/annotation2202131/range2202131/1

https://synbiohub.org/public/igem/BBa_K863111/annotation2202131/range2202131/1
type: Range
persistent identity: range2202131
display id: range2202131
version: 1
direction: direction/forward
top level: CBDclos
start: 93
end: 93
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2185120
display id: annotation2185120
version: 1
top level: CBDclos
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863111/annotation2185120/range2185120/1

https://synbiohub.org/public/igem/BBa_K863111/annotation2185120/range2185120/1
type: Range
persistent identity: range2185120
display id: range2185120
version: 1
direction: direction/forward
top level: CBDclos
start: 4
end: 9
orientation: inline

Cellulose Binding Protein
type: SequenceAnnotation
persistent identity: annotation2185126
display id: annotation2185126
version: 1
top level: CBDclos
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863111/annotation2185126/range2185126/1

https://synbiohub.org/public/igem/BBa_K863111/annotation2185126/range2185126/1
type: Range
persistent identity: range2185126
display id: range2185126
version: 1
direction: direction/forward
top level: CBDclos
start: 298
end: 303
orientation: inline

Cellulose binding domain (CBDclos)
type: SequenceAnnotation
persistent identity: annotation2185124
display id: annotation2185124
version: 1
top level: CBDclos
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K863111/annotation2185124/range2185124/1

https://synbiohub.org/public/igem/BBa_K863111/annotation2185124/range2185124/1
type: Range
persistent identity: range2185124
display id: range2185124
version: 1
direction: direction/forward
top level: CBDclos
start: 22
end: 297
orientation: inline

GS-Linker
type: SequenceAnnotation
persistent identity: annotation2185127
display id: annotation2185127
version: 1
top level: CBDclos
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863111/annotation2185127/range2185127/1

https://synbiohub.org/public/igem/BBa_K863111/annotation2185127/range2185127/1
type: Range
persistent identity: range2185127
display id: range2185127
version: 1
direction: direction/forward
top level: CBDclos
start: 304
end: 309
orientation: inline

BBa_K1333203
type: ComponentDefinition
persistent identity: BBa K1333203
display id: BBa_K1333203
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lambda cI-LGF2(D76A&D78A)
creator: Yanwen Xie
created: 2014-09-22T11:00:00Z
modified: 2015-05-08T01:09:58Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1708_
m user id: 0
owner id: 15838
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Fuse lambda cI with LGF2 mutated from Asp to Ala at sites 76 and 78 (the Glu which locates after three continuous Ala behind lambda repressor is at site 58). As one of the components in Bacterial Two-hybrid System, it causes little interaction with GAL11P compared to the normalized WT.
mutable notes: The LGF2 is mutated from GAC to GCC at site 76 and from GAC to GCC at site 78.
mutable provenance: It is from Agilent Technologies' BacterioMatch ⅡTwo-Hybrid System Vector Kit and modified by Site-Directed Mutagenesis(SDM).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: ATG start codon
sequence annotation: TGA stop codon
sequence annotation: LGF2(D76A&D78A)
sequence annotation: lambda cI
sequence: https://synbiohub.org/public/igem/BBa_K1333203_sequence/1

ATG start codon
type: SequenceAnnotation
persistent identity: annotation2385732
display id: annotation2385732
version: 1
top level: BBa_K1333203
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1333203/annotation2385732/range2385732/1

https://synbiohub.org/public/igem/BBa_K1333203/annotation2385732/range2385732/1
type: Range
persistent identity: range2385732
display id: range2385732
version: 1
direction: direction/forward
top level: BBa_K1333203
start: 1
end: 3
orientation: inline

TGA stop codon
type: SequenceAnnotation
persistent identity: annotation2385733
display id: annotation2385733
version: 1
top level: BBa_K1333203
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1333203/annotation2385733/range2385733/1

https://synbiohub.org/public/igem/BBa_K1333203/annotation2385733/range2385733/1
type: Range
persistent identity: range2385733
display id: range2385733
version: 1
direction: direction/forward
top level: BBa_K1333203
start: 841
end: 843
orientation: inline

LGF2(D76A&D78A)
type: SequenceAnnotation
persistent identity: annotation2385735
display id: annotation2385735
version: 1
top level: BBa_K1333203
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1333203/annotation2385735/range2385735/1

https://synbiohub.org/public/igem/BBa_K1333203/annotation2385735/range2385735/1
type: Range
persistent identity: range2385735
display id: range2385735
version: 1
direction: direction/forward
top level: BBa_K1333203
start: 721
end: 840
orientation: inline

lambda cI
type: SequenceAnnotation
persistent identity: annotation2385734
display id: annotation2385734
version: 1
top level: BBa_K1333203
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1333203/annotation2385734/range2385734/1

https://synbiohub.org/public/igem/BBa_K1333203/annotation2385734/range2385734/1
type: Range
persistent identity: range2385734
display id: range2385734
version: 1
direction: direction/forward
top level: BBa_K1333203
start: 1
end: 711
orientation: inline

BBa_K801039
type: ComponentDefinition
persistent identity: BBa K801039
display id: BBa_K801039
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SV40NLS-GAL4AD-linker-PIF3 (part for GAL4/LexA based light-switchable promoter system)
creator: Dong-Jiunn Jeffery TRUONG
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1057_
m user id: 0
owner id: 11507
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: CDS for a synthetic hybrid fusion protein. Consists of serveral domains. RFC10 pre and suffix
mutable notes: -
mutable provenance: CDS for a synthetic hybrid fusion protein
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: GAL4 activation domain (GAL4AD)
sequence annotation: SV40 nuclear localization sequence (recognized by importin alpha)
sequence annotation: T7 promoter (for sequencing)
sequence annotation: EcoRI/MfeI fusion scar
sequence annotation: HA epitope tag
sequence annotation: PIF3 (First 100 N-terminal amino acids)
sequence annotation: Linker
sequence: https://synbiohub.org/public/igem/BBa_K801039_sequence/1

GAL4 activation domain (GAL4AD)
type: SequenceAnnotation
persistent identity: annotation2199997
display id: annotation2199997
version: 1
top level: BBa_K801039
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K801039/annotation2199997/range2199997/1

https://synbiohub.org/public/igem/BBa_K801039/annotation2199997/range2199997/1
type: Range
persistent identity: range2199997
display id: range2199997
version: 1
direction: direction/forward
top level: BBa_K801039
start: 70
end: 408
orientation: inline

SV40 nuclear localization sequence (recognized by importin alpha)
type: SequenceAnnotation
persistent identity: annotation2199929
display id: annotation2199929
version: 1
top level: BBa_K801039
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801039/annotation2199929/range2199929/1

https://synbiohub.org/public/igem/BBa_K801039/annotation2199929/range2199929/1
type: Range
persistent identity: range2199929
display id: range2199929
version: 1
direction: direction/forward
top level: BBa_K801039
start: 31
end: 51
orientation: inline

T7 promoter (for sequencing)
type: SequenceAnnotation
persistent identity: annotation2200010
display id: annotation2200010
version: 1
top level: BBa_K801039
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K801039/annotation2200010/range2200010/1

https://synbiohub.org/public/igem/BBa_K801039/annotation2200010/range2200010/1
type: Range
persistent identity: range2200010
display id: range2200010
version: 1
direction: direction/forward
top level: BBa_K801039
start: 414
end: 436
orientation: inline

EcoRI/MfeI fusion scar
type: SequenceAnnotation
persistent identity: annotation2200000
display id: annotation2200000
version: 1
top level: BBa_K801039
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801039/annotation2200000/range2200000/1

https://synbiohub.org/public/igem/BBa_K801039/annotation2200000/range2200000/1
type: Range
persistent identity: range2200000
display id: range2200000
version: 1
direction: direction/forward
top level: BBa_K801039
start: 499
end: 504
orientation: inline

HA epitope tag
type: SequenceAnnotation
persistent identity: annotation2200012
display id: annotation2200012
version: 1
top level: BBa_K801039
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801039/annotation2200012/range2200012/1

https://synbiohub.org/public/igem/BBa_K801039/annotation2200012/range2200012/1
type: Range
persistent identity: range2200012
display id: range2200012
version: 1
direction: direction/forward
top level: BBa_K801039
start: 457
end: 477
orientation: inline

PIF3 (First 100 N-terminal amino acids)
type: SequenceAnnotation
persistent identity: annotation2199998
display id: annotation2199998
version: 1
top level: BBa_K801039
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K801039/annotation2199998/range2199998/1

https://synbiohub.org/public/igem/BBa_K801039/annotation2199998/range2199998/1
type: Range
persistent identity: range2199998
display id: range2199998
version: 1
direction: direction/forward
top level: BBa_K801039
start: 503
end: 801
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2200008
display id: annotation2200008
version: 1
top level: BBa_K801039
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801039/annotation2200008/range2200008/1

https://synbiohub.org/public/igem/BBa_K801039/annotation2200008/range2200008/1
type: Range
persistent identity: range2200008
display id: range2200008
version: 1
direction: direction/forward
top level: BBa_K801039
start: 409
end: 498
orientation: inline

BBa_K772001
type: ComponentDefinition
persistent identity: BBa K772001
display id: BBa_K772001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: EpCAM Binding Dock: C-terminus
creator: Mustafa Elitok
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:15Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1024_
m user id: 0
owner id: 9573
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: This gene sequence includes an EpCAM (Epithelial Cell Adhesion Molecule) antigen binding domain, OmpA transmembrane protein (dock) and TEV protease C terminus. It is planned to use this subpart with BBa_772, which contains only TEV protease N terminus differently instead of C terminus, in order to induce a signal reaction in the case of EpCAM presence. EpCAM is an natural antigen which is found high significantly in the cases of some breast and colorectal cancer subtypes. On the upstream region of protein sequence, there are a constitutive pLac promoter and appropiate RBS. To see how this part works: Experience.
mutable notes: There are two protein sequences between domains and the dock: One between the binding domain (antibody) and dock and one between the part of TEV protease.
mutable provenance: CD225 (EpCAM antibody) is commonly used for the test of presence of EpCAM in the laboratories. The sequences of OmpA and TEV protease terminuses are acquired from the Registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: TEV Protease C terminus
sequence annotation: Binding Dock
sequence annotation: pCons Promoter
sequence annotation: RBS
sequence annotation: OmpA (Dock)
sequence annotation: CD215 Antibody
sequence: https://synbiohub.org/public/igem/BBa_K772001_sequence/1

TEV Protease C terminus
type: SequenceAnnotation
persistent identity: annotation2203672
display id: annotation2203672
version: 1
top level: BBa_K772001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K772001/annotation2203672/range2203672/1

https://synbiohub.org/public/igem/BBa_K772001/annotation2203672/range2203672/1
type: Range
persistent identity: range2203672
display id: range2203672
version: 1
direction: direction/forward
top level: BBa_K772001
start: 94
end: 419
orientation: inline

Binding Dock
type: SequenceAnnotation
persistent identity: annotation2203674
display id: annotation2203674
version: 1
top level: BBa_K772001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K772001/annotation2203674/range2203674/1

https://synbiohub.org/public/igem/BBa_K772001/annotation2203674/range2203674/1
type: Range
persistent identity: range2203674
display id: range2203674
version: 1
direction: direction/forward
top level: BBa_K772001
start: 94
end: 1159
orientation: inline

pCons Promoter
type: SequenceAnnotation
persistent identity: annotation2203669
display id: annotation2203669
version: 1
top level: BBa_K772001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K772001/annotation2203669/range2203669/1

https://synbiohub.org/public/igem/BBa_K772001/annotation2203669/range2203669/1
type: Range
persistent identity: range2203669
display id: range2203669
version: 1
direction: direction/forward
top level: BBa_K772001
start: 1
end: 72
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2203670
display id: annotation2203670
version: 1
top level: BBa_K772001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K772001/annotation2203670/range2203670/1

https://synbiohub.org/public/igem/BBa_K772001/annotation2203670/range2203670/1
type: Range
persistent identity: range2203670
display id: range2203670
version: 1
direction: direction/forward
top level: BBa_K772001
start: 73
end: 93
orientation: inline

OmpA (Dock)
type: SequenceAnnotation
persistent identity: annotation2203671
display id: annotation2203671
version: 1
top level: BBa_K772001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K772001/annotation2203671/range2203671/1

https://synbiohub.org/public/igem/BBa_K772001/annotation2203671/range2203671/1
type: Range
persistent identity: range2203671
display id: range2203671
version: 1
direction: direction/forward
top level: BBa_K772001
start: 420
end: 857
orientation: inline

CD215 Antibody
type: SequenceAnnotation
persistent identity: annotation2203673
display id: annotation2203673
version: 1
top level: BBa_K772001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K772001/annotation2203673/range2203673/1

https://synbiohub.org/public/igem/BBa_K772001/annotation2203673/range2203673/1
type: Range
persistent identity: range2203673
display id: range2203673
version: 1
direction: direction/forward
top level: BBa_K772001
start: 858
end: 1159
orientation: inline

BBa_J18935
type: ComponentDefinition
persistent identity: BBa J18935
display id: BBa_J18935
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FKBP ~ mCitrine
creator: Raik Gruenberg
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
discontinued: false
dominant: false
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: P03 expression cassette from Gruenberg et al. 2010 series of interaction reporter proteins. All proteins share the same architecture: * T7Start-domain1-5xGS-domain2-preSCsite-HisTag-T7Stop Only Domains1 and 2 vary and are taken from a list of protein interaction input and output (readout) parts. ===Expression=== Proteins were expressed in E. coli BL21 using T7 Polymerase and were purified by His-tag affinity. ===Reference=== * Gruenberg, Ferrar, van der Sloot, Serrano (2010): Building Blocks for Protein Interaction Devices
mutable notes: Constructs were assembled according to R.F.C. 25. All parts were codon optimized for E. coli.
mutable provenance: synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_J18922
component: BBa_J18913
component: BBa_J18912
component: BBa_B0105
component: BBa_J18919
component: BBa_J18931
component: BBa_J18925
component: BBa_J18909
sequence annotation: BBa_J18922
sequence annotation: BBa_B0105
sequence annotation: BBa_J18925
sequence annotation: BBa_J18931
sequence annotation: BBa_J18912
sequence annotation: BBa_J18909
sequence annotation: BBa_J18919
sequence annotation: BBa_J18913
sequence: https://synbiohub.org/public/igem/BBa_J18935_sequence/1

BBa_J18922
type: Component
persistent identity: component2066449
display id: component2066449
version: 1
top level: BBa_J18935
access: public
definition: https://synbiohub.org/public/igem/BBa_J18922/1

BBa_J18913
type: Component
persistent identity: component2066463
display id: component2066463
version: 1
top level: BBa_J18935
access: public
definition: https://synbiohub.org/public/igem/BBa_J18913/1

BBa_J18912
type: Component
persistent identity: component2066443
display id: component2066443
version: 1
top level: BBa_J18935
access: public
definition: https://synbiohub.org/public/igem/BBa_J18912/1

BBa_B0105
type: Component
persistent identity: component2066451
display id: component2066451
version: 1
top level: BBa_J18935
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_B0105
type: Component
persistent identity: component2066454
display id: component2066454
version: 1
top level: BBa_J18935
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_B0105
type: Component
persistent identity: component2066445
display id: component2066445
version: 1
top level: BBa_J18935
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_J18919
type: Component
persistent identity: component2066455
display id: component2066455
version: 1
top level: BBa_J18935
access: public
definition: https://synbiohub.org/public/igem/BBa_J18919/1

BBa_B0105
type: Component
persistent identity: component2066457
display id: component2066457
version: 1
top level: BBa_J18935
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_J18931
type: Component
persistent identity: component2066452
display id: component2066452
version: 1
top level: BBa_J18935
access: public
definition: https://synbiohub.org/public/igem/BBa_J18931/1

BBa_B0105
type: Component
persistent identity: component2066448
display id: component2066448
version: 1
top level: BBa_J18935
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_J18925
type: Component
persistent identity: component2066446
display id: component2066446
version: 1
top level: BBa_J18935
access: public
definition: FKBP12

BBa_J18909
type: Component
persistent identity: component2066459
display id: component2066459
version: 1
top level: BBa_J18935
access: public
definition: https://synbiohub.org/public/igem/BBa_J18909/1

BBa_B0105
type: Component
persistent identity: component2066461
display id: component2066461
version: 1
top level: BBa_J18935
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_J18922
type: SequenceAnnotation
persistent identity: annotation2066449
display id: annotation2066449
version: 1
top level: BBa_J18935
location: https://synbiohub.org/public/igem/BBa_J18935/annotation2066449/range2066449/1
component: BBa_J18922

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type: Range
persistent identity: range2066449
display id: range2066449
version: 1
direction: direction/reverse
top level: BBa_J18935
start: 417
end: 446
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066448
display id: annotation2066448
version: 1
top level: BBa_J18935
location: https://synbiohub.org/public/igem/BBa_J18935/annotation2066448/range2066448/1
component: BBa_B0105

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type: Range
persistent identity: range2066448
display id: range2066448
version: 1
direction: direction/reverse
top level: BBa_J18935
start: 411
end: 416
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066454
display id: annotation2066454
version: 1
top level: BBa_J18935
location: https://synbiohub.org/public/igem/BBa_J18935/annotation2066454/range2066454/1
component: BBa_B0105

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type: Range
persistent identity: range2066454
display id: range2066454
version: 1
direction: direction/reverse
top level: BBa_J18935
start: 1167
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orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066457
display id: annotation2066457
version: 1
top level: BBa_J18935
location: https://synbiohub.org/public/igem/BBa_J18935/annotation2066457/range2066457/1
component: BBa_B0105

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type: Range
persistent identity: range2066457
display id: range2066457
version: 1
direction: direction/reverse
top level: BBa_J18935
start: 1197
end: 1202
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066461
display id: annotation2066461
version: 1
top level: BBa_J18935
location: https://synbiohub.org/public/igem/BBa_J18935/annotation2066461/range2066461/1
component: BBa_B0105

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type: Range
persistent identity: range2066461
display id: range2066461
version: 1
direction: direction/reverse
top level: BBa_J18935
start: 1221
end: 1226
orientation: inline

BBa_J18925
type: SequenceAnnotation
persistent identity: annotation2066446
display id: annotation2066446
version: 1
top level: BBa_J18935
location: https://synbiohub.org/public/igem/BBa_J18935/annotation2066446/range2066446/1
component: BBa_J18925

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type: Range
persistent identity: range2066446
display id: range2066446
version: 1
direction: direction/reverse
top level: BBa_J18935
start: 90
end: 410
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066445
display id: annotation2066445
version: 1
top level: BBa_J18935
location: https://synbiohub.org/public/igem/BBa_J18935/annotation2066445/range2066445/1
component: BBa_B0105

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type: Range
persistent identity: range2066445
display id: range2066445
version: 1
direction: direction/reverse
top level: BBa_J18935
start: 84
end: 89
orientation: inline

BBa_J18931
type: SequenceAnnotation
persistent identity: annotation2066452
display id: annotation2066452
version: 1
top level: BBa_J18935
location: https://synbiohub.org/public/igem/BBa_J18935/annotation2066452/range2066452/1
component: BBa_J18931

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type: Range
persistent identity: range2066452
display id: range2066452
version: 1
direction: direction/reverse
top level: BBa_J18935
start: 453
end: 1166
orientation: inline

BBa_J18912
type: SequenceAnnotation
persistent identity: annotation2066443
display id: annotation2066443
version: 1
top level: BBa_J18935
location: https://synbiohub.org/public/igem/BBa_J18935/annotation2066443/range2066443/1
component: BBa_J18912

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type: Range
persistent identity: range2066443
display id: range2066443
version: 1
direction: direction/reverse
top level: BBa_J18935
start: 1
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orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066451
display id: annotation2066451
version: 1
top level: BBa_J18935
location: https://synbiohub.org/public/igem/BBa_J18935/annotation2066451/range2066451/1
component: BBa_B0105

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type: Range
persistent identity: range2066451
display id: range2066451
version: 1
direction: direction/reverse
top level: BBa_J18935
start: 447
end: 452
orientation: inline

BBa_J18909
type: SequenceAnnotation
persistent identity: annotation2066459
display id: annotation2066459
version: 1
top level: BBa_J18935
location: https://synbiohub.org/public/igem/BBa_J18935/annotation2066459/range2066459/1
component: BBa_J18909

https://synbiohub.org/public/igem/BBa_J18935/annotation2066459/range2066459/1
type: Range
persistent identity: range2066459
display id: range2066459
version: 1
direction: direction/reverse
top level: BBa_J18935
start: 1203
end: 1220
orientation: inline

BBa_J18919
type: SequenceAnnotation
persistent identity: annotation2066455
display id: annotation2066455
version: 1
top level: BBa_J18935
location: https://synbiohub.org/public/igem/BBa_J18935/annotation2066455/range2066455/1
component: BBa_J18919

https://synbiohub.org/public/igem/BBa_J18935/annotation2066455/range2066455/1
type: Range
persistent identity: range2066455
display id: range2066455
version: 1
direction: direction/reverse
top level: BBa_J18935
start: 1173
end: 1196
orientation: inline

BBa_J18913
type: SequenceAnnotation
persistent identity: annotation2066463
display id: annotation2066463
version: 1
top level: BBa_J18935
location: https://synbiohub.org/public/igem/BBa_J18935/annotation2066463/range2066463/1
component: BBa_J18913

https://synbiohub.org/public/igem/BBa_J18935/annotation2066463/range2066463/1
type: Range
persistent identity: range2066463
display id: range2066463
version: 1
direction: direction/reverse
top level: BBa_J18935
start: 1227
end: 1361
orientation: inline

SP-AntiNIP
type: ComponentDefinition
persistent identity: BBa K157027
display id: BBa_K157027
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SP-AntiNIP-GGGSlinker-Tm-nYFP
creator: iGEM Team Freiburg 2008
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Part consists of the signal peptide of Erbb1/EGF-receptor, which ensures the transport to the cell membrane scFV-antiNIP, GGGSflexlinker (extracellular parts), the trans-membrane region of the Erbb1/EGF-receptor and the N-terminal part of the split-fluorophor YFP(intracellular part).
mutable notes: none
mutable provenance: Derived by cloning of the parts BBa_K157001, BBa_K157003, BBa_K157013, BBa_K157002, BBa_K157008. For further information see the description of the parts mentioned above.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: Scar (Thr, Gly)
sequence annotation: SP-AntiNIP-GGGSlinker-Tm-nYFP
sequence annotation: NgoMI
sequence annotation: GGGS-Linker
sequence annotation: SP
sequence annotation: Tm
sequence annotation: Sc-Fv_anti-NIP
sequence annotation: AgeI
sequence annotation: N-Term_YFP
sequence: https://synbiohub.org/public/igem/BBa_K157027_sequence/1

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1992658
display id: annotation1992658
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157027/annotation1992658/range1992658/1

https://synbiohub.org/public/igem/BBa_K157027/annotation1992658/range1992658/1
type: Range
persistent identity: range1992658
display id: range1992658
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 874
end: 879
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1992650
display id: annotation1992650
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157027/annotation1992650/range1992650/1

https://synbiohub.org/public/igem/BBa_K157027/annotation1992650/range1992650/1
type: Range
persistent identity: range1992650
display id: range1992650
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 82
end: 87
orientation: inline

SP-AntiNIP-GGGSlinker-Tm-nYFP
type: SequenceAnnotation
persistent identity: annotation1990179
display id: annotation1990179
version: 1
top level: SP-AntiNIP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157027/annotation1990179/range1990179/1

https://synbiohub.org/public/igem/BBa_K157027/annotation1990179/range1990179/1
type: Range
persistent identity: range1990179
display id: range1990179
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 1
end: 1428
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1992659
display id: annotation1992659
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157027/annotation1992659/range1992659/1

https://synbiohub.org/public/igem/BBa_K157027/annotation1992659/range1992659/1
type: Range
persistent identity: range1992659
display id: range1992659
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 949
end: 954
orientation: inline

NgoMI
type: SequenceAnnotation
persistent identity: annotation1990177
display id: annotation1990177
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157027/annotation1990177/range1990177/1

https://synbiohub.org/public/igem/BBa_K157027/annotation1990177/range1990177/1
type: Range
persistent identity: range1990177
display id: range1990177
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 4
end: 9
orientation: inline

GGGS-Linker
type: SequenceAnnotation
persistent identity: annotation1992653
display id: annotation1992653
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157027/annotation1992653/range1992653/1

https://synbiohub.org/public/igem/BBa_K157027/annotation1992653/range1992653/1
type: Range
persistent identity: range1992653
display id: range1992653
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 829
end: 873
orientation: inline

SP
type: SequenceAnnotation
persistent identity: annotation1992649
display id: annotation1992649
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157027/annotation1992649/range1992649/1

https://synbiohub.org/public/igem/BBa_K157027/annotation1992649/range1992649/1
type: Range
persistent identity: range1992649
display id: range1992649
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 10
end: 81
orientation: inline

Tm
type: SequenceAnnotation
persistent identity: annotation1992655
display id: annotation1992655
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157027/annotation1992655/range1992655/1

https://synbiohub.org/public/igem/BBa_K157027/annotation1992655/range1992655/1
type: Range
persistent identity: range1992655
display id: range1992655
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 880
end: 948
orientation: inline

Sc-Fv_anti-NIP
type: SequenceAnnotation
persistent identity: annotation1992651
display id: annotation1992651
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157027/annotation1992651/range1992651/1

https://synbiohub.org/public/igem/BBa_K157027/annotation1992651/range1992651/1
type: Range
persistent identity: range1992651
display id: range1992651
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 88
end: 822
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation1990178
display id: annotation1990178
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157027/annotation1990178/range1990178/1

https://synbiohub.org/public/igem/BBa_K157027/annotation1990178/range1990178/1
type: Range
persistent identity: range1990178
display id: range1990178
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 1423
end: 1428
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1992652
display id: annotation1992652
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157027/annotation1992652/range1992652/1

https://synbiohub.org/public/igem/BBa_K157027/annotation1992652/range1992652/1
type: Range
persistent identity: range1992652
display id: range1992652
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 823
end: 828
orientation: inline

N-Term_YFP
type: SequenceAnnotation
persistent identity: annotation1992660
display id: annotation1992660
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157027/annotation1992660/range1992660/1

https://synbiohub.org/public/igem/BBa_K157027/annotation1992660/range1992660/1
type: Range
persistent identity: range1992660
display id: range1992660
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 955
end: 1422
orientation: inline

BBa_K404306
type: ComponentDefinition
persistent identity: BBa K404306
display id: BBa_K404306
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Z-EGFR-1907_SEG-Linker
creator: Freiburg Bioware 2010
created: 2010-08-27T11:00:00Z
modified: 2015-05-08T01:12:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _508_
m user id: 0
owner id: 7049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: SEG-linker
sequence annotation: Z-EGFR-1907
sequence: https://synbiohub.org/public/igem/BBa_K404306_sequence/1

SEG-linker
type: SequenceAnnotation
persistent identity: annotation2094758
display id: annotation2094758
version: 1
top level: BBa_K404306
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404306/annotation2094758/range2094758/1

https://synbiohub.org/public/igem/BBa_K404306/annotation2094758/range2094758/1
type: Range
persistent identity: range2094758
display id: range2094758
version: 1
direction: direction/forward
top level: BBa_K404306
start: 181
end: 288
orientation: inline

Z-EGFR-1907
type: SequenceAnnotation
persistent identity: annotation2094759
display id: annotation2094759
version: 1
top level: BBa_K404306
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K404306/annotation2094759/range2094759/1

https://synbiohub.org/public/igem/BBa_K404306/annotation2094759/range2094759/1
type: Range
persistent identity: range2094759
display id: range2094759
version: 1
direction: direction/forward
top level: BBa_K404306
start: 1
end: 175
orientation: inline

SP-AntiNIP
type: ComponentDefinition
persistent identity: BBa K157022
display id: BBa_K157022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SP-AntiNIP-GGGSlinker
creator: iGEM Team Freiburg 2008
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The scFv-anti-NIP (with a flexible GGGS-linker at the C-terminus) fused to the signal peptide of Erbb1/EGF-receptor.
mutable notes: none
mutable provenance: Derived by cloning of the parts BBa_K157001, BBa_K157003 and BBa_K157013. For further information see the description of the parts mentioned above.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: Sc-Fv_anti-NIP
sequence annotation: NgoMI
sequence annotation: SP-AntiNIP-GGGSlinker
sequence annotation: Scar (Thr, Gly)
sequence annotation: SP
sequence annotation: AgeI
sequence annotation: GGGS-Linker
sequence: https://synbiohub.org/public/igem/BBa_K157022_sequence/1

Sc-Fv_anti-NIP
type: SequenceAnnotation
persistent identity: annotation1991631
display id: annotation1991631
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157022/annotation1991631/range1991631/1

https://synbiohub.org/public/igem/BBa_K157022/annotation1991631/range1991631/1
type: Range
persistent identity: range1991631
display id: range1991631
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 88
end: 822
orientation: inline

NgoMI
type: SequenceAnnotation
persistent identity: annotation1990125
display id: annotation1990125
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157022/annotation1990125/range1990125/1

https://synbiohub.org/public/igem/BBa_K157022/annotation1990125/range1990125/1
type: Range
persistent identity: range1990125
display id: range1990125
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 4
end: 9
orientation: inline

SP-AntiNIP-GGGSlinker
type: SequenceAnnotation
persistent identity: annotation1990157
display id: annotation1990157
version: 1
top level: SP-AntiNIP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157022/annotation1990157/range1990157/1

https://synbiohub.org/public/igem/BBa_K157022/annotation1990157/range1990157/1
type: Range
persistent identity: range1990157
display id: range1990157
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 1
end: 879
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1991580
display id: annotation1991580
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157022/annotation1991580/range1991580/1

https://synbiohub.org/public/igem/BBa_K157022/annotation1991580/range1991580/1
type: Range
persistent identity: range1991580
display id: range1991580
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 82
end: 87
orientation: inline

SP
type: SequenceAnnotation
persistent identity: annotation1991579
display id: annotation1991579
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157022/annotation1991579/range1991579/1

https://synbiohub.org/public/igem/BBa_K157022/annotation1991579/range1991579/1
type: Range
persistent identity: range1991579
display id: range1991579
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 10
end: 81
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1991698
display id: annotation1991698
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157022/annotation1991698/range1991698/1

https://synbiohub.org/public/igem/BBa_K157022/annotation1991698/range1991698/1
type: Range
persistent identity: range1991698
display id: range1991698
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 823
end: 828
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation1990141
display id: annotation1990141
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157022/annotation1990141/range1990141/1

https://synbiohub.org/public/igem/BBa_K157022/annotation1990141/range1990141/1
type: Range
persistent identity: range1990141
display id: range1990141
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 874
end: 879
orientation: inline

GGGS-Linker
type: SequenceAnnotation
persistent identity: annotation1991699
display id: annotation1991699
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157022/annotation1991699/range1991699/1

https://synbiohub.org/public/igem/BBa_K157022/annotation1991699/range1991699/1
type: Range
persistent identity: range1991699
display id: range1991699
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 828
end: 873
orientation: inline

BBa_K772002
type: ComponentDefinition
persistent identity: BBa K772002
display id: BBa_K772002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: EpCAM Binding Dock: N terminus
creator: Mustafa Elitok
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:15Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1024_
m user id: 0
owner id: 9573
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: This gene sequence includes an EpCAM (Epithelial p? Cell Adhesion Molecule) antigen binding domain, OmpA transmembrane protein (dock) and TEV protease N terminus. It is planned to use this subpart with BBa_772, which contains only TEV protease C terminus differently instead of N terminus, in order to induce a signal reaction in the case of EpCAM presence. EpCAM is an natural antigen which is found high significantly in the cases of some breast and colorectal cancer subtypes. On the upstream region of protein sequence, there are a constitutive pLac promoter and appropiate RBS. To see how this part works: Experience.
mutable notes: There are two protein sequences between domains and the dock: One between the binding domain (antibody) and dock and one between the part of TEV protease.
mutable provenance: CD225 (EpCAM antibody) is commonly used for the test of presence of EpCAM in the laboratories. The sequences of OmpA and TEV protease terminuses are acquired from the Registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: RBS
sequence annotation: TEV Protease N terminus
sequence annotation: OmpA (Dock)
sequence annotation: pCons Promoter
sequence annotation: CD215 antibody
sequence annotation: Binding Dock
sequence: https://synbiohub.org/public/igem/BBa_K772002_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2203683
display id: annotation2203683
version: 1
top level: BBa_K772002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K772002/annotation2203683/range2203683/1

https://synbiohub.org/public/igem/BBa_K772002/annotation2203683/range2203683/1
type: Range
persistent identity: range2203683
display id: range2203683
version: 1
direction: direction/forward
top level: BBa_K772002
start: 73
end: 93
orientation: inline

TEV Protease N terminus
type: SequenceAnnotation
persistent identity: annotation2203685
display id: annotation2203685
version: 1
top level: BBa_K772002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K772002/annotation2203685/range2203685/1

https://synbiohub.org/public/igem/BBa_K772002/annotation2203685/range2203685/1
type: Range
persistent identity: range2203685
display id: range2203685
version: 1
direction: direction/forward
top level: BBa_K772002
start: 94
end: 398
orientation: inline

OmpA (Dock)
type: SequenceAnnotation
persistent identity: annotation2203682
display id: annotation2203682
version: 1
top level: BBa_K772002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K772002/annotation2203682/range2203682/1

https://synbiohub.org/public/igem/BBa_K772002/annotation2203682/range2203682/1
type: Range
persistent identity: range2203682
display id: range2203682
version: 1
direction: direction/forward
top level: BBa_K772002
start: 399
end: 836
orientation: inline

pCons Promoter
type: SequenceAnnotation
persistent identity: annotation2203681
display id: annotation2203681
version: 1
top level: BBa_K772002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K772002/annotation2203681/range2203681/1

https://synbiohub.org/public/igem/BBa_K772002/annotation2203681/range2203681/1
type: Range
persistent identity: range2203681
display id: range2203681
version: 1
direction: direction/forward
top level: BBa_K772002
start: 1
end: 72
orientation: inline

CD215 antibody
type: SequenceAnnotation
persistent identity: annotation2203686
display id: annotation2203686
version: 1
top level: BBa_K772002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K772002/annotation2203686/range2203686/1

https://synbiohub.org/public/igem/BBa_K772002/annotation2203686/range2203686/1
type: Range
persistent identity: range2203686
display id: range2203686
version: 1
direction: direction/forward
top level: BBa_K772002
start: 837
end: 1138
orientation: inline

Binding Dock
type: SequenceAnnotation
persistent identity: annotation2203684
display id: annotation2203684
version: 1
top level: BBa_K772002
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K772002/annotation2203684/range2203684/1

https://synbiohub.org/public/igem/BBa_K772002/annotation2203684/range2203684/1
type: Range
persistent identity: range2203684
display id: range2203684
version: 1
direction: direction/forward
top level: BBa_K772002
start: 94
end: 1138
orientation: inline

BBa_J18957
type: ComponentDefinition
persistent identity: BBa J18957
display id: BBa_J18957
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mCerulean ~ Lov2
creator: Raik Gruenberg
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
discontinued: false
dominant: false
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: P25 expression cassette from Gruenberg et al. 2010 series of interaction reporter proteins. All proteins share the same architecture: * T7Start-domain1-5xGS-domain2-preSCsite-HisTag-T7Stop Only Domains1 and 2 vary and are taken from a list of protein interaction input and output (readout) parts. ===Expression=== Proteins were expressed in E. coli BL21 using T7 Polymerase and were purified by His-tag affinity. ===Reference=== * Gruenberg, Ferrar, van der Sloot, Serrano (2010): Building Blocks for Protein Interaction Devices
mutable notes: Constructs were assembled according to R.F.C. 25. All parts were codon optimized for E. coli.
mutable provenance: synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0105
component: BBa_J18930
component: BBa_J18909
component: BBa_J18922
component: BBa_J18927
component: BBa_J18919
component: BBa_J18913
component: BBa_J18912
sequence annotation: BBa_J18927
sequence annotation: BBa_J18913
sequence annotation: BBa_B0105
sequence annotation: BBa_J18919
sequence annotation: BBa_J18912
sequence annotation: BBa_J18930
sequence annotation: BBa_J18922
sequence annotation: BBa_J18909
sequence: https://synbiohub.org/public/igem/BBa_J18957_sequence/1

BBa_B0105
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display id: component2066928
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BBa_B0105
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display id: component2066919
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access: public
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BBa_J18930
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display id: component2066917
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access: public
definition: https://synbiohub.org/public/igem/BBa_J18930/1

BBa_J18909
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display id: component2066930
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BBa_J18922
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display id: component2066920
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access: public
definition: https://synbiohub.org/public/igem/BBa_J18922/1

BBa_B0105
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display id: component2066922
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_J18927
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display id: component2066923
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access: public
definition: Lov2

BBa_B0105
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display id: component2066916
version: 1
top level: BBa_J18957
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_B0105
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display id: component2066925
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access: public
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BBa_J18919
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display id: component2066926
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access: public
definition: https://synbiohub.org/public/igem/BBa_J18919/1

BBa_J18913
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display id: component2066934
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_J18913/1

BBa_J18912
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display id: component2066914
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_J18912/1

BBa_B0105
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_J18927
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display id: range2066934
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direction: direction/reverse
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start: 1320
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BBa_B0105
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BBa_J18919
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BBa_B0105
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BBa_B0105
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BBa_B0105
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BBa_J18912
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BBa_J18930
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component: BBa_J18930

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BBa_B0105
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direction: direction/reverse
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BBa_J18922
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display id: annotation2066920
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BBa_B0105
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BBa_J18909
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direction: direction/reverse
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BBa_K1404008
type: ComponentDefinition
persistent identity: BBa K1404008
display id: BBa_K1404008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: p70-CsgA-His*2, double His-tagged curli generator
creator: Juliette Paillet, Alexandre Duprey, Emiel van der Kouwe
created: 2014-09-23T11:00:00Z
modified: 2015-05-08T01:10:17Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1782_
m user id: 0
owner id: 21195
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: temp
mutable notes: temp
mutable provenance: temp
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: BBa_K342000
sequence annotation: His tag
sequence annotation: linker
sequence annotation: CsgD signal
sequence annotation: Beta sheet2
sequence annotation: Linker
sequence annotation: CsgA-His2
sequence annotation: RBS Paris 2009
sequence annotation: SEC signal
sequence annotation: GCT into GCC
sequence annotation: Beta sheet5
sequence annotation: Beta sheet
sequence annotation: Beta sheet4
sequence: https://synbiohub.org/public/igem/BBa_K1404008_sequence/1

BBa_K342000
type: SequenceAnnotation
persistent identity: annotation2426507
display id: annotation2426507
version: 1
top level: BBa_K1404008
role: SO:0000167
role: feature/promoter
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type: Range
persistent identity: range2426507
display id: range2426507
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 1
end: 70
orientation: inline

His tag
type: SequenceAnnotation
persistent identity: annotation2426521
display id: annotation2426521
version: 1
top level: BBa_K1404008
role: SO:0000324
role: feature/tag
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type: Range
persistent identity: range2426521
display id: range2426521
version: 1
direction: direction/forward
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start: 587
end: 604
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation2426520
display id: annotation2426520
version: 1
top level: BBa_K1404008
role: SO:0000110
role: feature/misc
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type: Range
persistent identity: range2426520
display id: range2426520
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 575
end: 586
orientation: inline

CsgD signal
type: SequenceAnnotation
persistent identity: annotation2426511
display id: annotation2426511
version: 1
top level: BBa_K1404008
role: SO:0000324
role: feature/tag
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persistent identity: range2426511
display id: range2426511
version: 1
direction: direction/forward
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start: 152
end: 217
orientation: inline

Beta sheet2
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persistent identity: annotation2426513
display id: annotation2426513
version: 1
top level: BBa_K1404008
role: SO:0000110
role: feature/misc
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type: Range
persistent identity: range2426513
display id: range2426513
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 287
end: 352
orientation: inline

Linker
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persistent identity: annotation2426518
display id: annotation2426518
version: 1
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role: SO:0000110
role: feature/misc
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type: Range
persistent identity: range2426518
display id: range2426518
version: 1
direction: direction/forward
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start: 545
end: 556
orientation: inline

His tag
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persistent identity: annotation2426519
display id: annotation2426519
version: 1
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role: SO:0000324
role: feature/tag
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type: Range
persistent identity: range2426519
display id: range2426519
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 557
end: 574
orientation: inline

CsgA-His2
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persistent identity: annotation2426510
display id: annotation2426510
version: 1
top level: BBa_K1404008
role: feature/cds
role: SO:0000316
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type: Range
persistent identity: range2426510
display id: range2426510
version: 1
direction: direction/forward
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start: 92
end: 607
orientation: inline

RBS Paris 2009
type: SequenceAnnotation
persistent identity: annotation2426508
display id: annotation2426508
version: 1
top level: BBa_K1404008
role: feature/rbs
role: SO:0000139
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type: Range
persistent identity: range2426508
display id: range2426508
version: 1
direction: direction/forward
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start: 79
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orientation: inline

SEC signal
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persistent identity: annotation2426509
display id: annotation2426509
version: 1
top level: BBa_K1404008
role: SO:0000324
role: feature/tag
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type: Range
persistent identity: range2426509
display id: range2426509
version: 1
direction: direction/forward
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start: 92
end: 151
orientation: inline

GCT into GCC
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persistent identity: annotation2426516
display id: annotation2426516
version: 1
top level: BBa_K1404008
role: SO:0001059
role: feature/mutation
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type: Range
persistent identity: range2426516
display id: range2426516
version: 1
direction: direction/forward
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start: 461
end: 463
orientation: inline

Beta sheet5
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display id: annotation2426517
version: 1
top level: BBa_K1404008
role: SO:0000110
role: feature/misc
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persistent identity: range2426517
display id: range2426517
version: 1
direction: direction/forward
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end: 544
orientation: inline

Beta sheet
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display id: annotation2426514
version: 1
top level: BBa_K1404008
role: SO:0000110
role: feature/misc
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type: Range
persistent identity: range2426514
display id: range2426514
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 353
end: 421
orientation: inline

Beta sheet4
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persistent identity: annotation2426515
display id: annotation2426515
version: 1
top level: BBa_K1404008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426515/range2426515/1

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type: Range
persistent identity: range2426515
display id: range2426515
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 422
end: 487
orientation: inline

Beta sheet
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persistent identity: annotation2426512
display id: annotation2426512
version: 1
top level: BBa_K1404008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426512/range2426512/1

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type: Range
persistent identity: range2426512
display id: range2426512
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 218
end: 286
orientation: inline

BBa_K801034
type: ComponentDefinition
persistent identity: BBa K801034
display id: BBa_K801034
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PIF3-linker
creator: Dong-Jiunn Jeffery TRUONG
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
group u list: _1057_
m user id: 0
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: composite part of Bba_K365000 and Bba_K365005
mutable notes: -
mutable provenance: composite part of Bba_K365000 and Bba_K365005
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: start
sequence annotation: BBa_K365000
sequence annotation: T > A
sequence annotation: RFC25 fusion scar
sequence annotation: BBa_K365005
sequence: https://synbiohub.org/public/igem/BBa_K801034_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2198897
display id: annotation2198897
version: 1
top level: BBa_K801034
role: SO:0000318
role: feature/start
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type: Range
persistent identity: range2198897
display id: range2198897
version: 1
direction: direction/forward
top level: BBa_K801034
start: 1
end: 3
orientation: inline

BBa_K365000
type: SequenceAnnotation
persistent identity: annotation2198898
display id: annotation2198898
version: 1
top level: BBa_K801034
role: SO:0000804
role: feature/BioBrick
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type: Range
persistent identity: range2198898
display id: range2198898
version: 1
direction: direction/forward
top level: BBa_K801034
start: 1
end: 300
orientation: inline

T > A
type: SequenceAnnotation
persistent identity: annotation2198899
display id: annotation2198899
version: 1
top level: BBa_K801034
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K801034/annotation2198899/range2198899/1

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type: Range
persistent identity: range2198899
display id: range2198899
version: 1
direction: direction/forward
top level: BBa_K801034
start: 177
end: 177
orientation: inline

RFC25 fusion scar
type: SequenceAnnotation
persistent identity: annotation2198901
display id: annotation2198901
version: 1
top level: BBa_K801034
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801034/annotation2198901/range2198901/1

https://synbiohub.org/public/igem/BBa_K801034/annotation2198901/range2198901/1
type: Range
persistent identity: range2198901
display id: range2198901
version: 1
direction: direction/forward
top level: BBa_K801034
start: 301
end: 306
orientation: inline

BBa_K365005
type: SequenceAnnotation
persistent identity: annotation2198900
display id: annotation2198900
version: 1
top level: BBa_K801034
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K801034/annotation2198900/range2198900/1

https://synbiohub.org/public/igem/BBa_K801034/annotation2198900/range2198900/1
type: Range
persistent identity: range2198900
display id: range2198900
version: 1
direction: direction/forward
top level: BBa_K801034
start: 307
end: 366
orientation: inline

BBa_K1404006
type: ComponentDefinition
persistent identity: BBa K1404006
display id: BBa_K1404006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: p70-CsgA, curli generator
creator: Juliette Paillet, Alexandre Duprey, Emiel van der Kouwe
created: 2014-09-23T11:00:00Z
modified: 2015-05-08T01:10:17Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1782_
m user id: 0
owner id: 21195
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: temp
mutable notes: temp
mutable provenance: temp
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: Beta sheet2
sequence annotation: Beta sheet1
sequence annotation: Beta sheet3
sequence annotation: CsgG signal
sequence annotation: Beta sheet4
sequence annotation: mutation
sequence annotation: RBS Paris 2009
sequence annotation: CsgA-WT
sequence annotation: Beta sheet5
sequence annotation: Sec signal
sequence annotation: Part:BBa_K342000
sequence: https://synbiohub.org/public/igem/BBa_K1404006_sequence/1

Beta sheet2
type: SequenceAnnotation
persistent identity: annotation2425860
display id: annotation2425860
version: 1
top level: BBa_K1404006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425860/range2425860/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425860/range2425860/1
type: Range
persistent identity: range2425860
display id: range2425860
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 287
end: 352
orientation: inline

Beta sheet1
type: SequenceAnnotation
persistent identity: annotation2425859
display id: annotation2425859
version: 1
top level: BBa_K1404006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425859/range2425859/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425859/range2425859/1
type: Range
persistent identity: range2425859
display id: range2425859
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 218
end: 286
orientation: inline

Beta sheet3
type: SequenceAnnotation
persistent identity: annotation2425861
display id: annotation2425861
version: 1
top level: BBa_K1404006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425861/range2425861/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425861/range2425861/1
type: Range
persistent identity: range2425861
display id: range2425861
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 353
end: 421
orientation: inline

CsgG signal
type: SequenceAnnotation
persistent identity: annotation2425825
display id: annotation2425825
version: 1
top level: BBa_K1404006
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425825/range2425825/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425825/range2425825/1
type: Range
persistent identity: range2425825
display id: range2425825
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 152
end: 217
orientation: inline

Beta sheet4
type: SequenceAnnotation
persistent identity: annotation2425862
display id: annotation2425862
version: 1
top level: BBa_K1404006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425862/range2425862/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425862/range2425862/1
type: Range
persistent identity: range2425862
display id: range2425862
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 422
end: 487
orientation: inline

mutation
type: SequenceAnnotation
persistent identity: annotation2425844
display id: annotation2425844
version: 1
top level: BBa_K1404006
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425844/range2425844/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425844/range2425844/1
type: Range
persistent identity: range2425844
display id: range2425844
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 461
end: 463
orientation: inline

RBS Paris 2009
type: SequenceAnnotation
persistent identity: annotation2425875
display id: annotation2425875
version: 1
top level: BBa_K1404006
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425875/range2425875/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425875/range2425875/1
type: Range
persistent identity: range2425875
display id: range2425875
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 79
end: 91
orientation: inline

CsgA-WT
type: SequenceAnnotation
persistent identity: annotation2425824
display id: annotation2425824
version: 1
top level: BBa_K1404006
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425824/range2425824/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425824/range2425824/1
type: Range
persistent identity: range2425824
display id: range2425824
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 92
end: 547
orientation: inline

Beta sheet5
type: SequenceAnnotation
persistent identity: annotation2425863
display id: annotation2425863
version: 1
top level: BBa_K1404006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425863/range2425863/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425863/range2425863/1
type: Range
persistent identity: range2425863
display id: range2425863
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 488
end: 544
orientation: inline

Sec signal
type: SequenceAnnotation
persistent identity: annotation2425823
display id: annotation2425823
version: 1
top level: BBa_K1404006
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425823/range2425823/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425823/range2425823/1
type: Range
persistent identity: range2425823
display id: range2425823
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 92
end: 151
orientation: inline

Part:BBa_K342000
type: SequenceAnnotation
persistent identity: annotation2425822
display id: annotation2425822
version: 1
top level: BBa_K1404006
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425822/range2425822/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425822/range2425822/1
type: Range
persistent identity: range2425822
display id: range2425822
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 1
end: 70
orientation: inline

SP-AntiNIP
type: ComponentDefinition
persistent identity: BBa K157029
display id: BBa_K157029
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SP-AntiNIP-GGGSlinker-Tm-nCFP
creator: iGEM Team Freiburg 2008
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Part consists of the signal peptide of Erbb1/EGF-receptor, scFV-antiNIP, GGGSflexlinker (extracellular part), the trans-membrane region of the Erbb1/EGF-receptor and the C-terminal part of the split fluorophor YFP with a fluolinker joined to its N-Terminus (intracellular part).
mutable notes: none
mutable provenance: Derived by cloning of the parts BBa_K157001, BBa_K157003, BBa_K157013, BBa_K157002, BBa_K157006. For further information see the description of the parts mentioned above.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: Scar (Thr, Gly)
sequence annotation: SP
sequence annotation: Tm
sequence annotation: Sc-Fv_anti-NIP
sequence annotation: N-Term_CFP
sequence annotation: GGGS-Linker
sequence annotation: AgeI
sequence annotation: SP-AntiNIP-GGGSlinker-Tm-nCFP
sequence annotation: NgoMI
sequence: https://synbiohub.org/public/igem/BBa_K157029_sequence/1

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993301
display id: annotation1993301
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157029/annotation1993301/range1993301/1

https://synbiohub.org/public/igem/BBa_K157029/annotation1993301/range1993301/1
type: Range
persistent identity: range1993301
display id: range1993301
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 82
end: 87
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993307
display id: annotation1993307
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157029/annotation1993307/range1993307/1

https://synbiohub.org/public/igem/BBa_K157029/annotation1993307/range1993307/1
type: Range
persistent identity: range1993307
display id: range1993307
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 949
end: 954
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993303
display id: annotation1993303
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157029/annotation1993303/range1993303/1

https://synbiohub.org/public/igem/BBa_K157029/annotation1993303/range1993303/1
type: Range
persistent identity: range1993303
display id: range1993303
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 823
end: 828
orientation: inline

SP
type: SequenceAnnotation
persistent identity: annotation1993300
display id: annotation1993300
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157029/annotation1993300/range1993300/1

https://synbiohub.org/public/igem/BBa_K157029/annotation1993300/range1993300/1
type: Range
persistent identity: range1993300
display id: range1993300
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 10
end: 81
orientation: inline

Tm
type: SequenceAnnotation
persistent identity: annotation1993306
display id: annotation1993306
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157029/annotation1993306/range1993306/1

https://synbiohub.org/public/igem/BBa_K157029/annotation1993306/range1993306/1
type: Range
persistent identity: range1993306
display id: range1993306
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 880
end: 948
orientation: inline

Sc-Fv_anti-NIP
type: SequenceAnnotation
persistent identity: annotation1993302
display id: annotation1993302
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157029/annotation1993302/range1993302/1

https://synbiohub.org/public/igem/BBa_K157029/annotation1993302/range1993302/1
type: Range
persistent identity: range1993302
display id: range1993302
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 88
end: 822
orientation: inline

N-Term_CFP
type: SequenceAnnotation
persistent identity: annotation1993308
display id: annotation1993308
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157029/annotation1993308/range1993308/1

https://synbiohub.org/public/igem/BBa_K157029/annotation1993308/range1993308/1
type: Range
persistent identity: range1993308
display id: range1993308
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 955
end: 1419
orientation: inline

GGGS-Linker
type: SequenceAnnotation
persistent identity: annotation1993304
display id: annotation1993304
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157029/annotation1993304/range1993304/1

https://synbiohub.org/public/igem/BBa_K157029/annotation1993304/range1993304/1
type: Range
persistent identity: range1993304
display id: range1993304
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 829
end: 873
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation1990245
display id: annotation1990245
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157029/annotation1990245/range1990245/1

https://synbiohub.org/public/igem/BBa_K157029/annotation1990245/range1990245/1
type: Range
persistent identity: range1990245
display id: range1990245
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 1420
end: 1425
orientation: inline

SP-AntiNIP-GGGSlinker-Tm-nCFP
type: SequenceAnnotation
persistent identity: annotation1990253
display id: annotation1990253
version: 1
top level: SP-AntiNIP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157029/annotation1990253/range1990253/1

https://synbiohub.org/public/igem/BBa_K157029/annotation1990253/range1990253/1
type: Range
persistent identity: range1990253
display id: range1990253
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 1
end: 1425
orientation: inline

NgoMI
type: SequenceAnnotation
persistent identity: annotation1990244
display id: annotation1990244
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157029/annotation1990244/range1990244/1

https://synbiohub.org/public/igem/BBa_K157029/annotation1990244/range1990244/1
type: Range
persistent identity: range1990244
display id: range1990244
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 4
end: 9
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993305
display id: annotation1993305
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157029/annotation1993305/range1993305/1

https://synbiohub.org/public/igem/BBa_K157029/annotation1993305/range1993305/1
type: Range
persistent identity: range1993305
display id: range1993305
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 874
end: 879
orientation: inline

N4 gp8
type: ComponentDefinition
persistent identity: BBa M36548
display id: BBa_M36548
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: DNA Polymerase Inhibitor (via N4 coliphage gp8)
creator: Rashmi Sharma, Bryce Bajar, Kendrick Wang
created: 2011-12-07T12:00:00Z
modified: 2015-05-08T01:14:05Z
discontinued: false
dominant: false
experience: experience/None
group u list: _848_
m user id: 0
owner id: 11092
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: to be added
mutable notes: to be added
mutable provenance: to be added
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: N4 gp8
sequence: https://synbiohub.org/public/igem/BBa_M36548_sequence/1

N4 gp8
type: SequenceAnnotation
persistent identity: annotation2166525
display id: annotation2166525
version: 1
top level: N4 gp8
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_M36548/annotation2166525/range2166525/1

https://synbiohub.org/public/igem/BBa_M36548/annotation2166525/range2166525/1
type: Range
persistent identity: range2166525
display id: range2166525
version: 1
direction: direction/forward
top level: N4 gp8
start: 1
end: 216
orientation: inline

BBa_J18933
type: ComponentDefinition
persistent identity: BBa J18933
display id: BBa_J18933
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FKBP~GLucFrag1
creator: Raik Gruenberg
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: P01 expression cassette from Gruenberg et al. 2010 series of interaction reporter proteins. All proteins share the same architecture: * T7Start-domain1-5xGS-domain2-preSCsite-HisTag-T7Stop Only Domains1 and 2 vary and are taken from a list of protein interaction input and output (readout) parts. ===Expression=== Proteins were expressed in E. coli BL21 using T7 Polymerase and were purified by His-tag affinity. ===Reference=== * Gruenberg, Ferrar, van der Sloot, Serrano (2010): Building Blocks for Protein Interaction Devices
mutable notes: * Parts were assembled according to R.F.C. 25. * All parts were codon optimized for E. coli
mutable provenance: gene synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0105
component: BBa_J18913
component: BBa_J18919
component: BBa_J18909
component: BBa_J18922
component: BBa_J18912
component: BBa_J18928
component: BBa_J18925
sequence annotation: BBa_J18928
sequence annotation: BBa_B0105
sequence annotation: BBa_J18912
sequence annotation: BBa_J18922
sequence annotation: BBa_J18909
sequence annotation: BBa_J18925
sequence annotation: BBa_J18919
sequence annotation: BBa_J18913
sequence: https://synbiohub.org/public/igem/BBa_J18933_sequence/1

BBa_B0105
type: Component
persistent identity: component2066404
display id: component2066404
version: 1
top level: BBa_J18933
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_B0105
type: Component
persistent identity: component2066410
display id: component2066410
version: 1
top level: BBa_J18933
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_B0105
type: Component
persistent identity: component2066401
display id: component2066401
version: 1
top level: BBa_J18933
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_B0105
type: Component
persistent identity: component2066413
display id: component2066413
version: 1
top level: BBa_J18933
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_J18913
type: Component
persistent identity: component2066419
display id: component2066419
version: 1
top level: BBa_J18933
access: public
definition: https://synbiohub.org/public/igem/BBa_J18913/1

BBa_J18919
type: Component
persistent identity: component2066411
display id: component2066411
version: 1
top level: BBa_J18933
access: public
definition: https://synbiohub.org/public/igem/BBa_J18919/1

BBa_J18909
type: Component
persistent identity: component2066415
display id: component2066415
version: 1
top level: BBa_J18933
access: public
definition: https://synbiohub.org/public/igem/BBa_J18909/1

BBa_B0105
type: Component
persistent identity: component2066417
display id: component2066417
version: 1
top level: BBa_J18933
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_J18922
type: Component
persistent identity: component2066405
display id: component2066405
version: 1
top level: BBa_J18933
access: public
definition: https://synbiohub.org/public/igem/BBa_J18922/1

BBa_J18912
type: Component
persistent identity: component2066399
display id: component2066399
version: 1
top level: BBa_J18933
access: public
definition: https://synbiohub.org/public/igem/BBa_J18912/1

BBa_J18928
type: Component
persistent identity: component2066408
display id: component2066408
version: 1
top level: BBa_J18933
access: public
definition: GLucFrag1

BBa_B0105
type: Component
persistent identity: component2066407
display id: component2066407
version: 1
top level: BBa_J18933
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_J18925
type: Component
persistent identity: component2066402
display id: component2066402
version: 1
top level: BBa_J18933
access: public
definition: FKBP12

BBa_J18928
type: SequenceAnnotation
persistent identity: annotation2066408
display id: annotation2066408
version: 1
top level: BBa_J18933
location: https://synbiohub.org/public/igem/BBa_J18933/annotation2066408/range2066408/1
component: BBa_J18928

https://synbiohub.org/public/igem/BBa_J18933/annotation2066408/range2066408/1
type: Range
persistent identity: range2066408
display id: range2066408
version: 1
direction: direction/reverse
top level: BBa_J18933
start: 453
end: 728
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066407
display id: annotation2066407
version: 1
top level: BBa_J18933
location: https://synbiohub.org/public/igem/BBa_J18933/annotation2066407/range2066407/1
component: BBa_B0105

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BBa_B0105
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BBa_J18922
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BBa_J18909
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BBa_J18925
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BBa_B0105
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BBa_J18913
type: SequenceAnnotation
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display id: annotation2066419
version: 1
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location: https://synbiohub.org/public/igem/BBa_J18933/annotation2066419/range2066419/1
component: BBa_J18913

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BBa_J18937
type: ComponentDefinition
persistent identity: BBa J18937
display id: BBa_J18937
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FKBP ~ mCerulean
creator: Raik Gruenberg
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
discontinued: false
dominant: false
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: P05 expression cassette from Gruenberg et al. 2010 series of interaction reporter proteins. All proteins share the same architecture: * T7Start-domain1-5xGS-domain2-preSCsite-HisTag-T7Stop Only Domains1 and 2 vary and are taken from a list of protein interaction input and output (readout) parts. ===Expression=== Proteins were expressed in E. coli BL21 using T7 Polymerase and were purified by His-tag affinity. ===Reference=== * Gruenberg, Ferrar, van der Sloot, Serrano (2010): Building Blocks for Protein Interaction Devices
mutable notes: Constructs were assembled according to R.F.C. 25. All parts were codon optimized for E. coli.
mutable provenance: synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0105
component: BBa_J18919
component: BBa_J18922
component: BBa_J18913
component: BBa_J18909
component: BBa_J18930
component: BBa_J18925
component: BBa_J18912
sequence annotation: BBa_B0105
sequence annotation: BBa_J18912
sequence annotation: BBa_J18922
sequence annotation: BBa_J18909
sequence annotation: BBa_J18930
sequence annotation: BBa_J18919
sequence annotation: BBa_J18913
sequence annotation: BBa_J18925
sequence: https://synbiohub.org/public/igem/BBa_J18937_sequence/1

BBa_B0105
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display id: component2066470
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_J18919
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version: 1
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definition: https://synbiohub.org/public/igem/BBa_J18919/1

BBa_B0105
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display id: component2066476
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_B0105
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definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_B0105
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definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_B0105
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_J18922
type: Component
persistent identity: component2066471
display id: component2066471
version: 1
top level: BBa_J18937
access: public
definition: https://synbiohub.org/public/igem/BBa_J18922/1

BBa_J18913
type: Component
persistent identity: component2066485
display id: component2066485
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_J18913/1

BBa_B0105
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persistent identity: component2066467
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definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_J18909
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persistent identity: component2066481
display id: component2066481
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_J18909/1

BBa_J18930
type: Component
persistent identity: component2066474
display id: component2066474
version: 1
top level: BBa_J18937
access: public
definition: https://synbiohub.org/public/igem/BBa_J18930/1

BBa_J18925
type: Component
persistent identity: component2066468
display id: component2066468
version: 1
top level: BBa_J18937
access: public
definition: FKBP12

BBa_J18912
type: Component
persistent identity: component2066465
display id: component2066465
version: 1
top level: BBa_J18937
access: public
definition: https://synbiohub.org/public/igem/BBa_J18912/1

BBa_B0105
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type: SequenceAnnotation
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type: Range
persistent identity: range2066465
display id: range2066465
version: 1
direction: direction/reverse
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BBa_J18922
type: SequenceAnnotation
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version: 1
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component: BBa_J18922

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persistent identity: range2066471
display id: range2066471
version: 1
direction: direction/reverse
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BBa_B0105
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component: BBa_B0105

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persistent identity: range2066470
display id: range2066470
version: 1
direction: direction/reverse
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start: 411
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BBa_B0105
type: SequenceAnnotation
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component: BBa_B0105

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type: Range
persistent identity: range2066473
display id: range2066473
version: 1
direction: direction/reverse
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BBa_B0105
type: SequenceAnnotation
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version: 1
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type: Range
persistent identity: range2066479
display id: range2066479
version: 1
direction: direction/reverse
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persistent identity: annotation2066481
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version: 1
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persistent identity: range2066481
display id: range2066481
version: 1
direction: direction/reverse
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start: 1203
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type: SequenceAnnotation
persistent identity: annotation2066474
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version: 1
top level: BBa_J18937
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component: BBa_J18930

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type: Range
persistent identity: range2066474
display id: range2066474
version: 1
direction: direction/reverse
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start: 453
end: 1166
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BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066467
display id: annotation2066467
version: 1
top level: BBa_J18937
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component: BBa_B0105

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persistent identity: range2066467
display id: range2066467
version: 1
direction: direction/reverse
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start: 84
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orientation: inline

BBa_J18919
type: SequenceAnnotation
persistent identity: annotation2066477
display id: annotation2066477
version: 1
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location: https://synbiohub.org/public/igem/BBa_J18937/annotation2066477/range2066477/1
component: BBa_J18919

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type: Range
persistent identity: range2066477
display id: range2066477
version: 1
direction: direction/reverse
top level: BBa_J18937
start: 1173
end: 1196
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066476
display id: annotation2066476
version: 1
top level: BBa_J18937
location: https://synbiohub.org/public/igem/BBa_J18937/annotation2066476/range2066476/1
component: BBa_B0105

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type: Range
persistent identity: range2066476
display id: range2066476
version: 1
direction: direction/reverse
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start: 1167
end: 1172
orientation: inline

BBa_J18913
type: SequenceAnnotation
persistent identity: annotation2066485
display id: annotation2066485
version: 1
top level: BBa_J18937
location: https://synbiohub.org/public/igem/BBa_J18937/annotation2066485/range2066485/1
component: BBa_J18913

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type: Range
persistent identity: range2066485
display id: range2066485
version: 1
direction: direction/reverse
top level: BBa_J18937
start: 1227
end: 1361
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BBa_J18925
type: SequenceAnnotation
persistent identity: annotation2066468
display id: annotation2066468
version: 1
top level: BBa_J18937
location: https://synbiohub.org/public/igem/BBa_J18937/annotation2066468/range2066468/1
component: BBa_J18925

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type: Range
persistent identity: range2066468
display id: range2066468
version: 1
direction: direction/reverse
top level: BBa_J18937
start: 90
end: 410
orientation: inline

CBDcex(T7)
type: ComponentDefinition
persistent identity: BBa K863102
display id: BBa_K863102
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cellulose binding Domain of C. Fimi Exoglucanase with T7, RBS, GS-Linker (Freiburg-Standard)
creator: Moritz M??ller
created: 2012-09-18T11:00:00Z
modified: 2015-06-17T01:19:09Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1123_
m user id: 4206
owner id: 13080
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Cellulose binding domain of the [http://www.ncbi.nlm.nih.gov/nucleotide/327179207?report=genbank&log$=nucltop&blast_rank=3&RID=152ZCN0E01N Cellulomonas fimi ATCC 484 exoglucanase gene] in Freiburg-standard with T7, RBS, GS-Linker (Freiburg-Standard)
mutable notes: You can find additional info to the Cellulose binding domain here: [http://partsregistry.org/Part:BBa_K863101:Design CBDcex_Freiburg]
mutable provenance: Cloning-vector
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: T7 promoter
sequence annotation: Exoglucanase
sequence annotation: GS-Linker
sequence annotation: TAA
sequence annotation: B0034
sequence annotation: Cellulose binding domain (CBDcex)
sequence annotation: ATG
sequence annotation: BBa_K863101
sequence annotation: RBS
sequence annotation: BBa_K525998
sequence annotation: AgeI
sequence: https://synbiohub.org/public/igem/BBa_K863102_sequence/1

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2187503
display id: annotation2187503
version: 1
top level: CBDcex(T7)
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K863102/annotation2187503/range2187503/1

https://synbiohub.org/public/igem/BBa_K863102/annotation2187503/range2187503/1
type: Range
persistent identity: range2187503
display id: range2187503
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 1
end: 20
orientation: inline

Exoglucanase
type: SequenceAnnotation
persistent identity: annotation2187508
display id: annotation2187508
version: 1
top level: CBDcex(T7)
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863102/annotation2187508/range2187508/1

https://synbiohub.org/public/igem/BBa_K863102/annotation2187508/range2187508/1
type: Range
persistent identity: range2187508
display id: range2187508
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 38
end: 49
orientation: inline

GS-Linker
type: SequenceAnnotation
persistent identity: annotation2187512
display id: annotation2187512
version: 1
top level: CBDcex(T7)
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863102/annotation2187512/range2187512/1

https://synbiohub.org/public/igem/BBa_K863102/annotation2187512/range2187512/1
type: Range
persistent identity: range2187512
display id: range2187512
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 359
end: 364
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2187514
display id: annotation2187514
version: 1
top level: CBDcex(T7)
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863102/annotation2187514/range2187514/1

https://synbiohub.org/public/igem/BBa_K863102/annotation2187514/range2187514/1
type: Range
persistent identity: range2187514
display id: range2187514
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 371
end: 373
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2187505
display id: annotation2187505
version: 1
top level: CBDcex(T7)
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863102/annotation2187505/range2187505/1

https://synbiohub.org/public/igem/BBa_K863102/annotation2187505/range2187505/1
type: Range
persistent identity: range2187505
display id: range2187505
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 21
end: 32
orientation: inline

Cellulose binding domain (CBDcex)
type: SequenceAnnotation
persistent identity: annotation2187510
display id: annotation2187510
version: 1
top level: CBDcex(T7)
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K863102/annotation2187510/range2187510/1

https://synbiohub.org/public/igem/BBa_K863102/annotation2187510/range2187510/1
type: Range
persistent identity: range2187510
display id: range2187510
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 50
end: 349
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2187507
display id: annotation2187507
version: 1
top level: CBDcex(T7)
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863102/annotation2187507/range2187507/1

https://synbiohub.org/public/igem/BBa_K863102/annotation2187507/range2187507/1
type: Range
persistent identity: range2187507
display id: range2187507
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 35
end: 37
orientation: inline

Exoglucanase
type: SequenceAnnotation
persistent identity: annotation2187511
display id: annotation2187511
version: 1
top level: CBDcex(T7)
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863102/annotation2187511/range2187511/1

https://synbiohub.org/public/igem/BBa_K863102/annotation2187511/range2187511/1
type: Range
persistent identity: range2187511
display id: range2187511
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 350
end: 358
orientation: inline

BBa_K863101
type: SequenceAnnotation
persistent identity: annotation2187509
display id: annotation2187509
version: 1
top level: CBDcex(T7)
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863102/annotation2187509/range2187509/1

https://synbiohub.org/public/igem/BBa_K863102/annotation2187509/range2187509/1
type: Range
persistent identity: range2187509
display id: range2187509
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 38
end: 373
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2187506
display id: annotation2187506
version: 1
top level: CBDcex(T7)
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K863102/annotation2187506/range2187506/1

https://synbiohub.org/public/igem/BBa_K863102/annotation2187506/range2187506/1
type: Range
persistent identity: range2187506
display id: range2187506
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 24
end: 27
orientation: inline

BBa_K525998
type: SequenceAnnotation
persistent identity: annotation2187504
display id: annotation2187504
version: 1
top level: CBDcex(T7)
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863102/annotation2187504/range2187504/1

https://synbiohub.org/public/igem/BBa_K863102/annotation2187504/range2187504/1
type: Range
persistent identity: range2187504
display id: range2187504
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 1
end: 32
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2187513
display id: annotation2187513
version: 1
top level: CBDcex(T7)
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863102/annotation2187513/range2187513/1

https://synbiohub.org/public/igem/BBa_K863102/annotation2187513/range2187513/1
type: Range
persistent identity: range2187513
display id: range2187513
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 365
end: 370
orientation: inline

BBa_J18941
type: ComponentDefinition
persistent identity: BBa J18941
display id: BBa_J18941
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FRB ~ GLucFrag2
creator: Raik Gruenberg
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
discontinued: false
dominant: false
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: P09 expression cassette from Gruenberg et al. 2010 series of interaction reporter proteins. All proteins share the same architecture: * T7Start-domain1-5xGS-domain2-preSCsite-HisTag-T7Stop Only Domains1 and 2 vary and are taken from a list of protein interaction input and output (readout) parts. ===Expression=== Proteins were expressed in E. coli BL21 using T7 Polymerase and were purified by His-tag affinity. ===Reference=== * Gruenberg, Ferrar, van der Sloot, Serrano (2010): Building Blocks for Protein Interaction Devices
mutable notes: Constructs were assembled according to R.F.C. 25. All parts were codon optimized for E. coli.
mutable provenance: synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_J18922
component: BBa_B0105
component: BBa_J18919
component: BBa_J18929
component: BBa_J18909
component: BBa_J18913
component: BBa_J18912
component: BBa_J18926
sequence annotation: BBa_J18912
sequence annotation: BBa_B0105
sequence annotation: BBa_J18913
sequence annotation: BBa_J18919
sequence annotation: BBa_J18909
sequence annotation: BBa_J18922
sequence annotation: BBa_J18929
sequence annotation: BBa_J18926
sequence: https://synbiohub.org/public/igem/BBa_J18941_sequence/1

BBa_J18922
type: Component
persistent identity: component2066565
display id: component2066565
version: 1
top level: BBa_J18941
access: public
definition: https://synbiohub.org/public/igem/BBa_J18922/1

BBa_B0105
type: Component
persistent identity: component2066577
display id: component2066577
version: 1
top level: BBa_J18941
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_J18919
type: Component
persistent identity: component2066571
display id: component2066571
version: 1
top level: BBa_J18941
access: public
definition: https://synbiohub.org/public/igem/BBa_J18919/1

BBa_J18929
type: Component
persistent identity: component2066568
display id: component2066568
version: 1
top level: BBa_J18941
access: public
definition: GLucFrag2

BBa_J18909
type: Component
persistent identity: component2066575
display id: component2066575
version: 1
top level: BBa_J18941
access: public
definition: https://synbiohub.org/public/igem/BBa_J18909/1

BBa_J18913
type: Component
persistent identity: component2066579
display id: component2066579
version: 1
top level: BBa_J18941
access: public
definition: https://synbiohub.org/public/igem/BBa_J18913/1

BBa_J18912
type: Component
persistent identity: component2066559
display id: component2066559
version: 1
top level: BBa_J18941
access: public
definition: https://synbiohub.org/public/igem/BBa_J18912/1

BBa_J18926
type: Component
persistent identity: component2066562
display id: component2066562
version: 1
top level: BBa_J18941
access: public
definition: FRB

BBa_B0105
type: Component
persistent identity: component2066573
display id: component2066573
version: 1
top level: BBa_J18941
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_B0105
type: Component
persistent identity: component2066567
display id: component2066567
version: 1
top level: BBa_J18941
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_B0105
type: Component
persistent identity: component2066570
display id: component2066570
version: 1
top level: BBa_J18941
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_B0105
type: Component
persistent identity: component2066564
display id: component2066564
version: 1
top level: BBa_J18941
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_B0105
type: Component
persistent identity: component2066561
display id: component2066561
version: 1
top level: BBa_J18941
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_J18912
type: SequenceAnnotation
persistent identity: annotation2066559
display id: annotation2066559
version: 1
top level: BBa_J18941
location: https://synbiohub.org/public/igem/BBa_J18941/annotation2066559/range2066559/1
component: BBa_J18912

https://synbiohub.org/public/igem/BBa_J18941/annotation2066559/range2066559/1
type: Range
persistent identity: range2066559
display id: range2066559
version: 1
direction: direction/reverse
top level: BBa_J18941
start: 1
end: 83
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066561
display id: annotation2066561
version: 1
top level: BBa_J18941
location: https://synbiohub.org/public/igem/BBa_J18941/annotation2066561/range2066561/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18941/annotation2066561/range2066561/1
type: Range
persistent identity: range2066561
display id: range2066561
version: 1
direction: direction/reverse
top level: BBa_J18941
start: 84
end: 89
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066573
display id: annotation2066573
version: 1
top level: BBa_J18941
location: https://synbiohub.org/public/igem/BBa_J18941/annotation2066573/range2066573/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18941/annotation2066573/range2066573/1
type: Range
persistent identity: range2066573
display id: range2066573
version: 1
direction: direction/reverse
top level: BBa_J18941
start: 669
end: 674
orientation: inline

BBa_J18913
type: SequenceAnnotation
persistent identity: annotation2066579
display id: annotation2066579
version: 1
top level: BBa_J18941
location: https://synbiohub.org/public/igem/BBa_J18941/annotation2066579/range2066579/1
component: BBa_J18913

https://synbiohub.org/public/igem/BBa_J18941/annotation2066579/range2066579/1
type: Range
persistent identity: range2066579
display id: range2066579
version: 1
direction: direction/reverse
top level: BBa_J18941
start: 699
end: 833
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066577
display id: annotation2066577
version: 1
top level: BBa_J18941
location: https://synbiohub.org/public/igem/BBa_J18941/annotation2066577/range2066577/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18941/annotation2066577/range2066577/1
type: Range
persistent identity: range2066577
display id: range2066577
version: 1
direction: direction/reverse
top level: BBa_J18941
start: 693
end: 698
orientation: inline

BBa_J18919
type: SequenceAnnotation
persistent identity: annotation2066571
display id: annotation2066571
version: 1
top level: BBa_J18941
location: https://synbiohub.org/public/igem/BBa_J18941/annotation2066571/range2066571/1
component: BBa_J18919

https://synbiohub.org/public/igem/BBa_J18941/annotation2066571/range2066571/1
type: Range
persistent identity: range2066571
display id: range2066571
version: 1
direction: direction/reverse
top level: BBa_J18941
start: 645
end: 668
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066567
display id: annotation2066567
version: 1
top level: BBa_J18941
location: https://synbiohub.org/public/igem/BBa_J18941/annotation2066567/range2066567/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18941/annotation2066567/range2066567/1
type: Range
persistent identity: range2066567
display id: range2066567
version: 1
direction: direction/reverse
top level: BBa_J18941
start: 405
end: 410
orientation: inline

BBa_J18909
type: SequenceAnnotation
persistent identity: annotation2066575
display id: annotation2066575
version: 1
top level: BBa_J18941
location: https://synbiohub.org/public/igem/BBa_J18941/annotation2066575/range2066575/1
component: BBa_J18909

https://synbiohub.org/public/igem/BBa_J18941/annotation2066575/range2066575/1
type: Range
persistent identity: range2066575
display id: range2066575
version: 1
direction: direction/reverse
top level: BBa_J18941
start: 675
end: 692
orientation: inline

BBa_J18922
type: SequenceAnnotation
persistent identity: annotation2066565
display id: annotation2066565
version: 1
top level: BBa_J18941
location: https://synbiohub.org/public/igem/BBa_J18941/annotation2066565/range2066565/1
component: BBa_J18922

https://synbiohub.org/public/igem/BBa_J18941/annotation2066565/range2066565/1
type: Range
persistent identity: range2066565
display id: range2066565
version: 1
direction: direction/reverse
top level: BBa_J18941
start: 375
end: 404
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066564
display id: annotation2066564
version: 1
top level: BBa_J18941
location: https://synbiohub.org/public/igem/BBa_J18941/annotation2066564/range2066564/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18941/annotation2066564/range2066564/1
type: Range
persistent identity: range2066564
display id: range2066564
version: 1
direction: direction/reverse
top level: BBa_J18941
start: 369
end: 374
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066570
display id: annotation2066570
version: 1
top level: BBa_J18941
location: https://synbiohub.org/public/igem/BBa_J18941/annotation2066570/range2066570/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18941/annotation2066570/range2066570/1
type: Range
persistent identity: range2066570
display id: range2066570
version: 1
direction: direction/reverse
top level: BBa_J18941
start: 639
end: 644
orientation: inline

BBa_J18929
type: SequenceAnnotation
persistent identity: annotation2066568
display id: annotation2066568
version: 1
top level: BBa_J18941
location: https://synbiohub.org/public/igem/BBa_J18941/annotation2066568/range2066568/1
component: BBa_J18929

https://synbiohub.org/public/igem/BBa_J18941/annotation2066568/range2066568/1
type: Range
persistent identity: range2066568
display id: range2066568
version: 1
direction: direction/reverse
top level: BBa_J18941
start: 411
end: 638
orientation: inline

BBa_J18926
type: SequenceAnnotation
persistent identity: annotation2066562
display id: annotation2066562
version: 1
top level: BBa_J18941
location: https://synbiohub.org/public/igem/BBa_J18941/annotation2066562/range2066562/1
component: BBa_J18926

https://synbiohub.org/public/igem/BBa_J18941/annotation2066562/range2066562/1
type: Range
persistent identity: range2066562
display id: range2066562
version: 1
direction: direction/reverse
top level: BBa_J18941
start: 90
end: 368
orientation: inline

SP-AntiNIP
type: ComponentDefinition
persistent identity: BBa K157025
display id: BBa_K157025
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SP-AntiNIP-GGGSlinker-Tm-betaLactamase(Bla2)
creator: iGEM Team Freiburg 2008
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: A composite part consisting of the signal peptide of Erbb1/EGF-receptor, scFV-antiNIP, GGGSflexlinker (extracellular part), the trans-membrane region of the Erbb1/EGF-receptor and the C-terminal part of the split-enzyme beta-Lactamase (intracellular part).
mutable notes: none
mutable provenance: Derived by cloning of the parts BBa_K157001, BBa_K157003, BBa_K157013, BBa_K157002 and BBa_I757012. For further information see the description of the parts mentioned above.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: Tm
sequence annotation: Sc-Fv_anti-NIP
sequence annotation: SP-AntiNIP-GGGSlinker-Tm-betaLactamase(Bla2)
sequence annotation: SP
sequence annotation: AgeI
sequence annotation: Scar (Thr, Gly)
sequence annotation: Bla2
sequence annotation: GGGS-Linker
sequence annotation: NgoMI
sequence: https://synbiohub.org/public/igem/BBa_K157025_sequence/1

Tm
type: SequenceAnnotation
persistent identity: annotation1992634
display id: annotation1992634
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157025/annotation1992634/range1992634/1

https://synbiohub.org/public/igem/BBa_K157025/annotation1992634/range1992634/1
type: Range
persistent identity: range1992634
display id: range1992634
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 880
end: 948
orientation: inline

Sc-Fv_anti-NIP
type: SequenceAnnotation
persistent identity: annotation1992630
display id: annotation1992630
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157025/annotation1992630/range1992630/1

https://synbiohub.org/public/igem/BBa_K157025/annotation1992630/range1992630/1
type: Range
persistent identity: range1992630
display id: range1992630
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 88
end: 822
orientation: inline

SP-AntiNIP-GGGSlinker-Tm-betaLactamase(Bla2)
type: SequenceAnnotation
persistent identity: annotation1992626
display id: annotation1992626
version: 1
top level: SP-AntiNIP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157025/annotation1992626/range1992626/1

https://synbiohub.org/public/igem/BBa_K157025/annotation1992626/range1992626/1
type: Range
persistent identity: range1992626
display id: range1992626
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 1
end: 1230
orientation: inline

SP
type: SequenceAnnotation
persistent identity: annotation1992628
display id: annotation1992628
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157025/annotation1992628/range1992628/1

https://synbiohub.org/public/igem/BBa_K157025/annotation1992628/range1992628/1
type: Range
persistent identity: range1992628
display id: range1992628
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 10
end: 81
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation1992637
display id: annotation1992637
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157025/annotation1992637/range1992637/1

https://synbiohub.org/public/igem/BBa_K157025/annotation1992637/range1992637/1
type: Range
persistent identity: range1992637
display id: range1992637
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 1225
end: 1230
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1992629
display id: annotation1992629
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157025/annotation1992629/range1992629/1

https://synbiohub.org/public/igem/BBa_K157025/annotation1992629/range1992629/1
type: Range
persistent identity: range1992629
display id: range1992629
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 82
end: 87
orientation: inline

Bla2
type: SequenceAnnotation
persistent identity: annotation1992636
display id: annotation1992636
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157025/annotation1992636/range1992636/1

https://synbiohub.org/public/igem/BBa_K157025/annotation1992636/range1992636/1
type: Range
persistent identity: range1992636
display id: range1992636
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 955
end: 1224
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1992631
display id: annotation1992631
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157025/annotation1992631/range1992631/1

https://synbiohub.org/public/igem/BBa_K157025/annotation1992631/range1992631/1
type: Range
persistent identity: range1992631
display id: range1992631
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 823
end: 828
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1992633
display id: annotation1992633
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157025/annotation1992633/range1992633/1

https://synbiohub.org/public/igem/BBa_K157025/annotation1992633/range1992633/1
type: Range
persistent identity: range1992633
display id: range1992633
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 874
end: 879
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1992635
display id: annotation1992635
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157025/annotation1992635/range1992635/1

https://synbiohub.org/public/igem/BBa_K157025/annotation1992635/range1992635/1
type: Range
persistent identity: range1992635
display id: range1992635
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 949
end: 954
orientation: inline

GGGS-Linker
type: SequenceAnnotation
persistent identity: annotation1992632
display id: annotation1992632
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157025/annotation1992632/range1992632/1

https://synbiohub.org/public/igem/BBa_K157025/annotation1992632/range1992632/1
type: Range
persistent identity: range1992632
display id: range1992632
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 829
end: 873
orientation: inline

NgoMI
type: SequenceAnnotation
persistent identity: annotation1992627
display id: annotation1992627
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157025/annotation1992627/range1992627/1

https://synbiohub.org/public/igem/BBa_K157025/annotation1992627/range1992627/1
type: Range
persistent identity: range1992627
display id: range1992627
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 4
end: 9
orientation: inline

BBa_K1994008
type: ComponentDefinition
persistent identity: BBa K1994008
display id: BBa_K1994008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PP7 RNA binding protein
creator: Liam Carroll
created: 2016-09-21T11:00:00Z
modified: 2016-10-19T07:35:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2461_
m user id: 30870
owner id: 27126
owning group id: 9
status: status/Planning
bookmark: false
mutable description: RNA binding protein, recognises and binds RNA sequence:
mutable notes: No special considerations
mutable provenance: PP7 bacteriophage
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1994008_sequence/1

BBa_K1404007
type: ComponentDefinition
persistent identity: BBa K1404007
display id: BBa_K1404007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: p70-CsgA-His, His-tagged curli generator
creator: Juliette Paillet, Alexandre Duprey, Emiel van der Kouwe
created: 2014-09-23T11:00:00Z
modified: 2015-05-08T01:10:17Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1782_
m user id: 0
owner id: 21195
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: temp
mutable notes: temp
mutable provenance: temp
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: Beta sheet2
sequence annotation: Beta sheet4
sequence annotation: CsgG signal
sequence annotation: RBS Paris 2009
sequence annotation: His tag
sequence annotation: Beta sheet5
sequence annotation: CsgA-His1
sequence annotation: Linker
sequence annotation: BBa_K342000
sequence annotation: Sec signal
sequence annotation: Beta sheet 1
sequence annotation: Beta sheet3
sequence annotation: GCT into GCC
sequence: https://synbiohub.org/public/igem/BBa_K1404007_sequence/1

Beta sheet2
type: SequenceAnnotation
persistent identity: annotation2426224
display id: annotation2426224
version: 1
top level: BBa_K1404007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426224/range2426224/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426224/range2426224/1
type: Range
persistent identity: range2426224
display id: range2426224
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 287
end: 352
orientation: inline

Beta sheet4
type: SequenceAnnotation
persistent identity: annotation2426226
display id: annotation2426226
version: 1
top level: BBa_K1404007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426226/range2426226/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426226/range2426226/1
type: Range
persistent identity: range2426226
display id: range2426226
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 422
end: 487
orientation: inline

CsgG signal
type: SequenceAnnotation
persistent identity: annotation2426222
display id: annotation2426222
version: 1
top level: BBa_K1404007
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426222/range2426222/1

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type: Range
persistent identity: range2426222
display id: range2426222
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 152
end: 217
orientation: inline

RBS Paris 2009
type: SequenceAnnotation
persistent identity: annotation2426220
display id: annotation2426220
version: 1
top level: BBa_K1404007
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426220/range2426220/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426220/range2426220/1
type: Range
persistent identity: range2426220
display id: range2426220
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 79
end: 91
orientation: inline

His tag
type: SequenceAnnotation
persistent identity: annotation2426230
display id: annotation2426230
version: 1
top level: BBa_K1404007
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426230/range2426230/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426230/range2426230/1
type: Range
persistent identity: range2426230
display id: range2426230
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 557
end: 574
orientation: inline

Beta sheet5
type: SequenceAnnotation
persistent identity: annotation2426228
display id: annotation2426228
version: 1
top level: BBa_K1404007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426228/range2426228/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426228/range2426228/1
type: Range
persistent identity: range2426228
display id: range2426228
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 488
end: 544
orientation: inline

CsgA-His1
type: SequenceAnnotation
persistent identity: annotation2426219
display id: annotation2426219
version: 1
top level: BBa_K1404007
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426219/range2426219/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426219/range2426219/1
type: Range
persistent identity: range2426219
display id: range2426219
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 92
end: 577
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2426229
display id: annotation2426229
version: 1
top level: BBa_K1404007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426229/range2426229/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426229/range2426229/1
type: Range
persistent identity: range2426229
display id: range2426229
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 545
end: 556
orientation: inline

BBa_K342000
type: SequenceAnnotation
persistent identity: annotation2426218
display id: annotation2426218
version: 1
top level: BBa_K1404007
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426218/range2426218/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426218/range2426218/1
type: Range
persistent identity: range2426218
display id: range2426218
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 1
end: 70
orientation: inline

Sec signal
type: SequenceAnnotation
persistent identity: annotation2426221
display id: annotation2426221
version: 1
top level: BBa_K1404007
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426221/range2426221/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426221/range2426221/1
type: Range
persistent identity: range2426221
display id: range2426221
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 92
end: 151
orientation: inline

Beta sheet 1
type: SequenceAnnotation
persistent identity: annotation2426223
display id: annotation2426223
version: 1
top level: BBa_K1404007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426223/range2426223/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426223/range2426223/1
type: Range
persistent identity: range2426223
display id: range2426223
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 218
end: 286
orientation: inline

Beta sheet3
type: SequenceAnnotation
persistent identity: annotation2426225
display id: annotation2426225
version: 1
top level: BBa_K1404007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426225/range2426225/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426225/range2426225/1
type: Range
persistent identity: range2426225
display id: range2426225
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 353
end: 421
orientation: inline

GCT into GCC
type: SequenceAnnotation
persistent identity: annotation2426227
display id: annotation2426227
version: 1
top level: BBa_K1404007
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426227/range2426227/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426227/range2426227/1
type: Range
persistent identity: range2426227
display id: range2426227
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 461
end: 463
orientation: inline

GLucFrag2
type: ComponentDefinition
persistent identity: BBa J18929
display id: BBa_J18929
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: hGaussia Luciferase fragment 2
creator: Raik Gruenberg
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: C-terminal fragment of H. gaussia luciferase for PCA (detection of protein-protein interactions). See part J18928 for details.
mutable notes: * gene synthesis * codon optimized for E. coli
mutable provenance: gene synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_J18929_sequence/1

BBa_J18956
type: ComponentDefinition
persistent identity: BBa J18956
display id: BBa_J18956
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lov2 ~ mCerulean
creator: Raik Gruenberg
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
discontinued: false
dominant: false
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: P24 expression cassette from Gruenberg et al. 2010 series of interaction reporter proteins. All proteins share the same architecture: * T7Start-domain1-5xGS-domain2-preSCsite-HisTag-T7Stop Only Domains1 and 2 vary and are taken from a list of protein interaction input and output (readout) parts. ===Expression=== Proteins were expressed in E. coli BL21 using T7 Polymerase and were purified by His-tag affinity. ===Reference=== * Gruenberg, Ferrar, van der Sloot, Serrano (2010): Building Blocks for Protein Interaction Devices
mutable notes: Constructs were assembled according to R.F.C. 25. All parts were codon optimized for E. coli.
mutable provenance: synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0105
component: BBa_J18912
component: BBa_J18913
component: BBa_J18909
component: BBa_J18927
component: BBa_J18930
component: BBa_J18922
component: BBa_J18919
sequence annotation: BBa_J18913
sequence annotation: BBa_J18927
sequence annotation: BBa_B0105
sequence annotation: BBa_J18930
sequence annotation: BBa_J18912
sequence annotation: BBa_J18919
sequence annotation: BBa_J18922
sequence annotation: BBa_J18909
sequence: https://synbiohub.org/public/igem/BBa_J18956_sequence/1

BBa_B0105
type: Component
persistent identity: component2066950
display id: component2066950
version: 1
top level: BBa_J18956
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_B0105
type: Component
persistent identity: component2066941
display id: component2066941
version: 1
top level: BBa_J18956
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_J18912
type: Component
persistent identity: component2066936
display id: component2066936
version: 1
top level: BBa_J18956
access: public
definition: https://synbiohub.org/public/igem/BBa_J18912/1

BBa_J18913
type: Component
persistent identity: component2066956
display id: component2066956
version: 1
top level: BBa_J18956
access: public
definition: https://synbiohub.org/public/igem/BBa_J18913/1

BBa_B0105
type: Component
persistent identity: component2066954
display id: component2066954
version: 1
top level: BBa_J18956
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_J18909
type: Component
persistent identity: component2066952
display id: component2066952
version: 1
top level: BBa_J18956
access: public
definition: https://synbiohub.org/public/igem/BBa_J18909/1

BBa_J18927
type: Component
persistent identity: component2066939
display id: component2066939
version: 1
top level: BBa_J18956
access: public
definition: Lov2

BBa_J18930
type: Component
persistent identity: component2066945
display id: component2066945
version: 1
top level: BBa_J18956
access: public
definition: https://synbiohub.org/public/igem/BBa_J18930/1

BBa_B0105
type: Component
persistent identity: component2066938
display id: component2066938
version: 1
top level: BBa_J18956
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_B0105
type: Component
persistent identity: component2066944
display id: component2066944
version: 1
top level: BBa_J18956
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_J18922
type: Component
persistent identity: component2066942
display id: component2066942
version: 1
top level: BBa_J18956
access: public
definition: https://synbiohub.org/public/igem/BBa_J18922/1

BBa_J18919
type: Component
persistent identity: component2066948
display id: component2066948
version: 1
top level: BBa_J18956
access: public
definition: https://synbiohub.org/public/igem/BBa_J18919/1

BBa_B0105
type: Component
persistent identity: component2066947
display id: component2066947
version: 1
top level: BBa_J18956
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_J18913
type: SequenceAnnotation
persistent identity: annotation2066956
display id: annotation2066956
version: 1
top level: BBa_J18956
location: https://synbiohub.org/public/igem/BBa_J18956/annotation2066956/range2066956/1
component: BBa_J18913

https://synbiohub.org/public/igem/BBa_J18956/annotation2066956/range2066956/1
type: Range
persistent identity: range2066956
display id: range2066956
version: 1
direction: direction/reverse
top level: BBa_J18956
start: 1320
end: 1454
orientation: inline

BBa_J18927
type: SequenceAnnotation
persistent identity: annotation2066939
display id: annotation2066939
version: 1
top level: BBa_J18956
location: https://synbiohub.org/public/igem/BBa_J18956/annotation2066939/range2066939/1
component: BBa_J18927

https://synbiohub.org/public/igem/BBa_J18956/annotation2066939/range2066939/1
type: Range
persistent identity: range2066939
display id: range2066939
version: 1
direction: direction/reverse
top level: BBa_J18956
start: 90
end: 503
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066938
display id: annotation2066938
version: 1
top level: BBa_J18956
location: https://synbiohub.org/public/igem/BBa_J18956/annotation2066938/range2066938/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18956/annotation2066938/range2066938/1
type: Range
persistent identity: range2066938
display id: range2066938
version: 1
direction: direction/reverse
top level: BBa_J18956
start: 84
end: 89
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066947
display id: annotation2066947
version: 1
top level: BBa_J18956
location: https://synbiohub.org/public/igem/BBa_J18956/annotation2066947/range2066947/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18956/annotation2066947/range2066947/1
type: Range
persistent identity: range2066947
display id: range2066947
version: 1
direction: direction/reverse
top level: BBa_J18956
start: 1260
end: 1265
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066941
display id: annotation2066941
version: 1
top level: BBa_J18956
location: https://synbiohub.org/public/igem/BBa_J18956/annotation2066941/range2066941/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18956/annotation2066941/range2066941/1
type: Range
persistent identity: range2066941
display id: range2066941
version: 1
direction: direction/reverse
top level: BBa_J18956
start: 504
end: 509
orientation: inline

BBa_J18930
type: SequenceAnnotation
persistent identity: annotation2066945
display id: annotation2066945
version: 1
top level: BBa_J18956
location: https://synbiohub.org/public/igem/BBa_J18956/annotation2066945/range2066945/1
component: BBa_J18930

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type: Range
persistent identity: range2066945
display id: range2066945
version: 1
direction: direction/reverse
top level: BBa_J18956
start: 546
end: 1259
orientation: inline

BBa_J18912
type: SequenceAnnotation
persistent identity: annotation2066936
display id: annotation2066936
version: 1
top level: BBa_J18956
location: https://synbiohub.org/public/igem/BBa_J18956/annotation2066936/range2066936/1
component: BBa_J18912

https://synbiohub.org/public/igem/BBa_J18956/annotation2066936/range2066936/1
type: Range
persistent identity: range2066936
display id: range2066936
version: 1
direction: direction/reverse
top level: BBa_J18956
start: 1
end: 83
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066944
display id: annotation2066944
version: 1
top level: BBa_J18956
location: https://synbiohub.org/public/igem/BBa_J18956/annotation2066944/range2066944/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18956/annotation2066944/range2066944/1
type: Range
persistent identity: range2066944
display id: range2066944
version: 1
direction: direction/reverse
top level: BBa_J18956
start: 540
end: 545
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066954
display id: annotation2066954
version: 1
top level: BBa_J18956
location: https://synbiohub.org/public/igem/BBa_J18956/annotation2066954/range2066954/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_J18956/annotation2066954/range2066954/1
type: Range
persistent identity: range2066954
display id: range2066954
version: 1
direction: direction/reverse
top level: BBa_J18956
start: 1314
end: 1319
orientation: inline

BBa_J18919
type: SequenceAnnotation
persistent identity: annotation2066948
display id: annotation2066948
version: 1
top level: BBa_J18956
location: https://synbiohub.org/public/igem/BBa_J18956/annotation2066948/range2066948/1
component: BBa_J18919

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type: Range
persistent identity: range2066948
display id: range2066948
version: 1
direction: direction/reverse
top level: BBa_J18956
start: 1266
end: 1289
orientation: inline

BBa_J18922
type: SequenceAnnotation
persistent identity: annotation2066942
display id: annotation2066942
version: 1
top level: BBa_J18956
location: https://synbiohub.org/public/igem/BBa_J18956/annotation2066942/range2066942/1
component: BBa_J18922

https://synbiohub.org/public/igem/BBa_J18956/annotation2066942/range2066942/1
type: Range
persistent identity: range2066942
display id: range2066942
version: 1
direction: direction/reverse
top level: BBa_J18956
start: 510
end: 539
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2066950
display id: annotation2066950
version: 1
top level: BBa_J18956
location: https://synbiohub.org/public/igem/BBa_J18956/annotation2066950/range2066950/1
component: BBa_B0105

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type: Range
persistent identity: range2066950
display id: range2066950
version: 1
direction: direction/reverse
top level: BBa_J18956
start: 1290
end: 1295
orientation: inline

BBa_J18909
type: SequenceAnnotation
persistent identity: annotation2066952
display id: annotation2066952
version: 1
top level: BBa_J18956
location: https://synbiohub.org/public/igem/BBa_J18956/annotation2066952/range2066952/1
component: BBa_J18909

https://synbiohub.org/public/igem/BBa_J18956/annotation2066952/range2066952/1
type: Range
persistent identity: range2066952
display id: range2066952
version: 1
direction: direction/reverse
top level: BBa_J18956
start: 1296
end: 1313
orientation: inline

BBa_J18953
type: ComponentDefinition
persistent identity: BBa J18953
display id: BBa_J18953
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ZipE34 ~ mCherry
creator: Raik Gruenberg
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
discontinued: false
dominant: false
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: P21 expression cassette from Gruenberg et al. 2010 series of interaction reporter proteins. All proteins share the same architecture: * T7Start-domain1-5xGS-domain2-preSCsite-HisTag-T7Stop Only Domains1 and 2 vary and are taken from a list of protein interaction input and output (readout) parts. ===Expression=== Proteins were expressed in E. coli BL21 using T7 Polymerase and were purified by His-tag affinity. ===Reference=== * Gruenberg, Ferrar, van der Sloot, Serrano (2010): Building Blocks for Protein Interaction Devices
mutable notes: Constructs were assembled according to R.F.C. 25. All parts were codon optimized for E. coli.
mutable provenance: synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_J18909
component: BBa_B0105
component: BBa_J18922
component: BBa_J18932
component: BBa_J18923
component: BBa_J18913
component: BBa_J18912
component: BBa_J18919
sequence annotation: BBa_B0105
sequence annotation: BBa_J18909
sequence annotation: BBa_J18912
sequence annotation: BBa_J18919
sequence annotation: BBa_J18913
sequence annotation: BBa_J18923
sequence annotation: BBa_J18932
sequence annotation: BBa_J18922
sequence: https://synbiohub.org/public/igem/BBa_J18953_sequence/1

BBa_J18909
type: Component
persistent identity: component2066842
display id: component2066842
version: 1
top level: BBa_J18953
access: public
definition: https://synbiohub.org/public/igem/BBa_J18909/1

BBa_B0105
type: Component
persistent identity: component2066828
display id: component2066828
version: 1
top level: BBa_J18953
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_B0105
type: Component
persistent identity: component2066837
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BBa_J18948
type: ComponentDefinition
persistent identity: BBa J18948
display id: BBa_J18948
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lov2 ~ GLucFrag2
creator: Raik Gruenberg
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
discontinued: false
dominant: false
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: P16 expression cassette from Gruenberg et al. 2010 series of interaction reporter proteins. All proteins share the same architecture: * T7Start-domain1-5xGS-domain2-preSCsite-HisTag-T7Stop Only Domains1 and 2 vary and are taken from a list of protein interaction input and output (readout) parts. ===Expression=== Proteins were expressed in E. coli BL21 using T7 Polymerase and were purified by His-tag affinity. ===Reference=== * Gruenberg, Ferrar, van der Sloot, Serrano (2010): Building Blocks for Protein Interaction Devices
mutable notes: Constructs were assembled according to R.F.C. 25. All parts were codon optimized for E. coli.
mutable provenance: synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
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component: BBa_J18922
component: BBa_J18919
component: BBa_J18913
component: BBa_J18929
component: BBa_J18912
component: BBa_J18927
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sequence annotation: BBa_J18922
sequence annotation: BBa_J18912
sequence annotation: BBa_J18927
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sequence annotation: BBa_J18929
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BBa_K863113
type: ComponentDefinition
persistent identity: BBa K863113
display id: BBa_K863113
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cellulose binding Domain of C. cellulovorans cellulose binding protein with Reporter GFP
creator: Moritz M??ller
created: 2012-09-19T11:00:00Z
modified: 2015-06-17T01:20:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1123_
m user id: 4206
owner id: 13080
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Cellulose binding Domain from Clostridium cellulovorans [http://www.ncbi.nlm.nih.gov/nuccore/M73817 cellulose binding protein gene] with T7, RBS linked with two amino acids (GS) to a GFP with His-tag.
mutable notes: CBDclos fused to a his-tagged GFP in a Freiburg-manner
mutable provenance: Cloning-vector and [http://partsregistry.org/Part:BBa_K863121 BBa_K863121]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Cellulose Binding Protein
sequence annotation: BBa_K863112
sequence annotation: TAA
sequence annotation: T7 promoter
sequence annotation: BBa_K525998
sequence annotation: ATG
sequence annotation: GS-Linker
sequence annotation: BBa_E0040
sequence annotation: Silent mutation (A original)
sequence annotation: Cellulose binding domain (CBDclos)
sequence annotation: B0034
sequence annotation: RBS
sequence annotation: His-Tag
sequence annotation: AgeI
sequence annotation: BBa_K863111
sequence annotation: BBa_K863121
sequence annotation: Freiburg-Scar
sequence: https://synbiohub.org/public/igem/BBa_K863113_sequence/1

Cellulose Binding Protein
type: SequenceAnnotation
persistent identity: annotation2188395
display id: annotation2188395
version: 1
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role: feature/conserved
role: SO:0000330
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version: 1
top level: BBa_K863113
role: SO:0000804
role: feature/BioBrick
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https://synbiohub.org/public/igem/BBa_K863113/annotation2188386/range2188386/1
type: Range
persistent identity: range2188386
display id: range2188386
version: 1
direction: direction/reverse
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start: 1
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orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2188402
display id: annotation2188402
version: 1
top level: BBa_K863113
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188402/range2188402/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188402/range2188402/1
type: Range
persistent identity: range2188402
display id: range2188402
version: 1
direction: direction/forward
top level: BBa_K863113
start: 1073
end: 1075
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2188388
display id: annotation2188388
version: 1
top level: BBa_K863113
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188388/range2188388/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188388/range2188388/1
type: Range
persistent identity: range2188388
display id: range2188388
version: 1
direction: direction/forward
top level: BBa_K863113
start: 1
end: 20
orientation: inline

BBa_K525998
type: SequenceAnnotation
persistent identity: annotation2188387
display id: annotation2188387
version: 1
top level: BBa_K863113
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188387/range2188387/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188387/range2188387/1
type: Range
persistent identity: range2188387
display id: range2188387
version: 1
direction: direction/forward
top level: BBa_K863113
start: 1
end: 32
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2188391
display id: annotation2188391
version: 1
top level: BBa_K863113
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188391/range2188391/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188391/range2188391/1
type: Range
persistent identity: range2188391
display id: range2188391
version: 1
direction: direction/forward
top level: BBa_K863113
start: 35
end: 38
orientation: inline

GS-Linker
type: SequenceAnnotation
persistent identity: annotation2188396
display id: annotation2188396
version: 1
top level: BBa_K863113
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188396/range2188396/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188396/range2188396/1
type: Range
persistent identity: range2188396
display id: range2188396
version: 1
direction: direction/forward
top level: BBa_K863113
start: 323
end: 328
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2188399
display id: annotation2188399
version: 1
top level: BBa_K863113
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188399/range2188399/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188399/range2188399/1
type: Range
persistent identity: range2188399
display id: range2188399
version: 1
direction: direction/forward
top level: BBa_K863113
start: 335
end: 1048
orientation: inline

Silent mutation (A original)
type: SequenceAnnotation
persistent identity: annotation2200501
display id: annotation2200501
version: 1
top level: BBa_K863113
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2200501/range2200501/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2200501/range2200501/1
type: Range
persistent identity: range2200501
display id: range2200501
version: 1
direction: direction/forward
top level: BBa_K863113
start: 112
end: 112
orientation: inline

Cellulose binding domain (CBDclos)
type: SequenceAnnotation
persistent identity: annotation2188394
display id: annotation2188394
version: 1
top level: BBa_K863113
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188394/range2188394/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188394/range2188394/1
type: Range
persistent identity: range2188394
display id: range2188394
version: 1
direction: direction/forward
top level: BBa_K863113
start: 41
end: 316
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2188389
display id: annotation2188389
version: 1
top level: BBa_K863113
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188389/range2188389/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188389/range2188389/1
type: Range
persistent identity: range2188389
display id: range2188389
version: 1
direction: direction/forward
top level: BBa_K863113
start: 21
end: 32
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2188390
display id: annotation2188390
version: 1
top level: BBa_K863113
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188390/range2188390/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188390/range2188390/1
type: Range
persistent identity: range2188390
display id: range2188390
version: 1
direction: direction/forward
top level: BBa_K863113
start: 24
end: 27
orientation: inline

His-Tag
type: SequenceAnnotation
persistent identity: annotation2188400
display id: annotation2188400
version: 1
top level: BBa_K863113
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188400/range2188400/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188400/range2188400/1
type: Range
persistent identity: range2188400
display id: range2188400
version: 1
direction: direction/forward
top level: BBa_K863113
start: 1049
end: 1066
orientation: inline

Cellulose Binding Protein
type: SequenceAnnotation
persistent identity: annotation2188392
display id: annotation2188392
version: 1
top level: BBa_K863113
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188392/range2188392/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188392/range2188392/1
type: Range
persistent identity: range2188392
display id: range2188392
version: 1
direction: direction/forward
top level: BBa_K863113
start: 35
end: 40
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2188401
display id: annotation2188401
version: 1
top level: BBa_K863113
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188401/range2188401/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188401/range2188401/1
type: Range
persistent identity: range2188401
display id: range2188401
version: 1
direction: direction/forward
top level: BBa_K863113
start: 1068
end: 1072
orientation: inline

BBa_K863111
type: SequenceAnnotation
persistent identity: annotation2188393
display id: annotation2188393
version: 1
top level: BBa_K863113
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188393/range2188393/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188393/range2188393/1
type: Range
persistent identity: range2188393
display id: range2188393
version: 1
direction: direction/forward
top level: BBa_K863113
start: 35
end: 328
orientation: inline

BBa_K863121
type: SequenceAnnotation
persistent identity: annotation2188398
display id: annotation2188398
version: 1
top level: BBa_K863113
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188398/range2188398/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188398/range2188398/1
type: Range
persistent identity: range2188398
display id: range2188398
version: 1
direction: direction/reverse
top level: BBa_K863113
start: 335
end: 1075
orientation: inline

Freiburg-Scar
type: SequenceAnnotation
persistent identity: annotation2188397
display id: annotation2188397
version: 1
top level: BBa_K863113
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188397/range2188397/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188397/range2188397/1
type: Range
persistent identity: range2188397
display id: range2188397
version: 1
direction: direction/forward
top level: BBa_K863113
start: 329
end: 334
orientation: inline