//plasmidbackbone/version/_5
type: Collection
persistent identity: plasmidbackbone version 5
display id: plasmidbackbone_version__5
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_K864013
member: BB base ve
member: pSB8K15
member: BBa_K864000
member: pSB8C15FRT
member: pSB4A5
member: BBa_K864005
member: BBa_K864014
member: BBa_K864012
member: BBa_K864006
member: pSB8A15FRT
member: pSB8S15
member: pSB4K50
member: BBa_I51000
member: BBa_K864015
member: pSB4K5
member: pSB4T5
member: pSB4C50
member: BBa_K864011
member: BBa_I51001
member: BBa_K864002
member: pSB3C5
member: BBa_K864007
member: BBa_I51019
member: BBa_K864010
member: pSB4A50
member: BBa_K864003
member: BBa_K864008
member: pSB8A15
member: pSB8S15FRT
member: pSB3K5
member: BBa_K864001
member: pSB3T5
member: pSB4C5
member: pSB8C15
member: BBa_K864004
member: BBa_K864009
member: pSB8K15FRT

BBa_I51000
type: ComponentDefinition
persistent identity: BBa I51000
display id: BBa_I51000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BioBrick base vector, as originally designed
creator: Reshma Shetty
created: 2006-03-13T12:00:00Z
modified: 2015-08-31T04:07:42Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
component: BBa_B0054
component: BBa_B0062
component: BBa_G00102
component: BBa_B0055
component: BBa_B0053
component: BBa_B0044
component: BBa_G00001
component: BBa_B0045
component: BBa_B0042
component: BBa_G00000
component: BBa_B0043
component: BBa_I52000
component: BBa_P1006
component: BBa_G00100
sequence annotation: BBa_B0043
sequence annotation: BBa_P1006
sequence annotation: BBa_G00000
sequence annotation: BBa_B0044
sequence annotation: BBa_B0042
sequence annotation: BBa_B0062
sequence annotation: BBa_G00100
sequence annotation: BBa_B0053
sequence annotation: BBa_B0055
sequence annotation: BBa_B0045
sequence annotation: BBa_G00102
sequence annotation: BBa_B0054
sequence annotation: BBa_I52000
sequence annotation: BBa_G00001
sequence: https://synbiohub.org/public/igem/BBa_I51000_sequence/1

BBa_B0054
type: Component
persistent identity: component2236075
display id: component2236075
version: 1
top level: BBa_I51000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0054/1

BBa_B0062
type: Component
persistent identity: component2236079
display id: component2236079
version: 1
top level: BBa_I51000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0062/1

BBa_G00102
type: Component
persistent identity: component2236077
display id: component2236077
version: 1
top level: BBa_I51000
access: public
definition: https://synbiohub.org/public/igem/BBa_G00102/1

BBa_B0055
type: Component
persistent identity: component2236095
display id: component2236095
version: 1
top level: BBa_I51000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0055/1

BBa_B0053
type: Component
persistent identity: component2236090
display id: component2236090
version: 1
top level: BBa_I51000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0053/1

BBa_B0044
type: Component
persistent identity: component2236065
display id: component2236065
version: 1
top level: BBa_I51000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0044/1

BBa_G00001
type: Component
persistent identity: component2236060
display id: component2236060
version: 1
top level: BBa_I51000
access: public
definition: https://synbiohub.org/public/igem/BBa_G00001/1

BBa_B0045
type: Component
persistent identity: component2236088
display id: component2236088
version: 1
top level: BBa_I51000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0045/1

BBa_B0042
type: Component
persistent identity: component2236102
display id: component2236102
version: 1
top level: BBa_I51000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_G00000
type: Component
persistent identity: component2236112
display id: component2236112
version: 1
top level: BBa_I51000
access: public
definition: https://synbiohub.org/public/igem/BBa_G00000/1

BBa_B0043
type: Component
persistent identity: component2236107
display id: component2236107
version: 1
top level: BBa_I51000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0043/1

BBa_B0042
type: Component
persistent identity: component2236072
display id: component2236072
version: 1
top level: BBa_I51000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_B0045
type: Component
persistent identity: component2236081
display id: component2236081
version: 1
top level: BBa_I51000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0045/1

BBa_I52000
type: Component
persistent identity: component2236127
display id: component2236127
version: 1
top level: BBa_I51000
access: public
definition: https://synbiohub.org/public/igem/BBa_I52000/1

BBa_P1006
type: Component
persistent identity: component2236086
display id: component2236086
version: 1
top level: BBa_I51000
access: public
definition: https://synbiohub.org/public/igem/BBa_P1006/1

BBa_G00100
type: Component
persistent identity: component2236092
display id: component2236092
version: 1
top level: BBa_I51000
access: public
definition: https://synbiohub.org/public/igem/BBa_G00100/1

BBa_B0043
type: SequenceAnnotation
persistent identity: annotation2236107
display id: annotation2236107
version: 1
top level: BBa_I51000
location: https://synbiohub.org/public/igem/BBa_I51000/annotation2236107/range2236107/1
component: BBa_B0043

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persistent identity: range2236107
display id: range2236107
version: 1
direction: direction/reverse
top level: BBa_I51000
start: 1314
end: 1326
orientation: inline

BBa_P1006
type: SequenceAnnotation
persistent identity: annotation2236086
display id: annotation2236086
version: 1
top level: BBa_I51000
location: https://synbiohub.org/public/igem/BBa_I51000/annotation2236086/range2236086/1
component: BBa_P1006

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type: Range
persistent identity: range2236086
display id: range2236086
version: 1
direction: direction/reverse
top level: BBa_I51000
start: 183
end: 1125
orientation: inline

BBa_G00000
type: SequenceAnnotation
persistent identity: annotation2236112
display id: annotation2236112
version: 1
top level: BBa_I51000
location: https://synbiohub.org/public/igem/BBa_I51000/annotation2236112/range2236112/1
component: BBa_G00000

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type: Range
persistent identity: range2236112
display id: range2236112
version: 1
direction: direction/reverse
top level: BBa_I51000
start: 1327
end: 1348
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BBa_B0044
type: SequenceAnnotation
persistent identity: annotation2236065
display id: annotation2236065
version: 1
top level: BBa_I51000
location: https://synbiohub.org/public/igem/BBa_I51000/annotation2236065/range2236065/1
component: BBa_B0044

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type: Range
persistent identity: range2236065
display id: range2236065
version: 1
direction: direction/reverse
top level: BBa_I51000
start: 22
end: 34
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2236102
display id: annotation2236102
version: 1
top level: BBa_I51000
location: https://synbiohub.org/public/igem/BBa_I51000/annotation2236102/range2236102/1
component: BBa_B0042

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type: Range
persistent identity: range2236102
display id: range2236102
version: 1
direction: direction/reverse
top level: BBa_I51000
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orientation: inline

BBa_B0062
type: SequenceAnnotation
persistent identity: annotation2236079
display id: annotation2236079
version: 1
top level: BBa_I51000
location: https://synbiohub.org/public/igem/BBa_I51000/annotation2236079/range2236079/1
component: BBa_B0062

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persistent identity: range2236079
display id: range2236079
version: 1
direction: direction/reverse
top level: BBa_I51000
start: 136
end: 176
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2236072
display id: annotation2236072
version: 1
top level: BBa_I51000
location: https://synbiohub.org/public/igem/BBa_I51000/annotation2236072/range2236072/1
component: BBa_B0042

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type: Range
persistent identity: range2236072
display id: range2236072
version: 1
direction: direction/reverse
top level: BBa_I51000
start: 35
end: 46
orientation: inline

BBa_G00100
type: SequenceAnnotation
persistent identity: annotation2236092
display id: annotation2236092
version: 1
top level: BBa_I51000
location: https://synbiohub.org/public/igem/BBa_I51000/annotation2236092/range2236092/1
component: BBa_G00100

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type: Range
persistent identity: range2236092
display id: range2236092
version: 1
direction: direction/reverse
top level: BBa_I51000
start: 1204
end: 1223
orientation: inline

BBa_B0053
type: SequenceAnnotation
persistent identity: annotation2236090
display id: annotation2236090
version: 1
top level: BBa_I51000
location: https://synbiohub.org/public/igem/BBa_I51000/annotation2236090/range2236090/1
component: BBa_B0053

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type: Range
persistent identity: range2236090
display id: range2236090
version: 1
direction: direction/reverse
top level: BBa_I51000
start: 1132
end: 1203
orientation: inline

BBa_B0055
type: SequenceAnnotation
persistent identity: annotation2236095
display id: annotation2236095
version: 1
top level: BBa_I51000
location: https://synbiohub.org/public/igem/BBa_I51000/annotation2236095/range2236095/1
component: BBa_B0055

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type: Range
persistent identity: range2236095
display id: range2236095
version: 1
direction: direction/reverse
top level: BBa_I51000
start: 1224
end: 1301
orientation: inline

BBa_B0045
type: SequenceAnnotation
persistent identity: annotation2236088
display id: annotation2236088
version: 1
top level: BBa_I51000
location: https://synbiohub.org/public/igem/BBa_I51000/annotation2236088/range2236088/1
component: BBa_B0045

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type: Range
persistent identity: range2236088
display id: range2236088
version: 1
direction: direction/reverse
top level: BBa_I51000
start: 1126
end: 1131
orientation: inline

BBa_B0045
type: SequenceAnnotation
persistent identity: annotation2236081
display id: annotation2236081
version: 1
top level: BBa_I51000
location: https://synbiohub.org/public/igem/BBa_I51000/annotation2236081/range2236081/1
component: BBa_B0045

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type: Range
persistent identity: range2236081
display id: range2236081
version: 1
direction: direction/reverse
top level: BBa_I51000
start: 177
end: 182
orientation: inline

BBa_G00102
type: SequenceAnnotation
persistent identity: annotation2236077
display id: annotation2236077
version: 1
top level: BBa_I51000
location: https://synbiohub.org/public/igem/BBa_I51000/annotation2236077/range2236077/1
component: BBa_G00102

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type: Range
persistent identity: range2236077
display id: range2236077
version: 1
direction: direction/reverse
top level: BBa_I51000
start: 116
end: 135
orientation: inline

BBa_B0054
type: SequenceAnnotation
persistent identity: annotation2236075
display id: annotation2236075
version: 1
top level: BBa_I51000
location: https://synbiohub.org/public/igem/BBa_I51000/annotation2236075/range2236075/1
component: BBa_B0054

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type: Range
persistent identity: range2236075
display id: range2236075
version: 1
direction: direction/reverse
top level: BBa_I51000
start: 47
end: 115
orientation: inline

BBa_I52000
type: SequenceAnnotation
persistent identity: annotation2236127
display id: annotation2236127
version: 1
top level: BBa_I51000
location: https://synbiohub.org/public/igem/BBa_I51000/annotation2236127/range2236127/1
component: BBa_I52000

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type: Range
persistent identity: range2236127
display id: range2236127
version: 1
direction: direction/reverse
top level: BBa_I51000
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orientation: inline

BBa_G00001
type: SequenceAnnotation
persistent identity: annotation2236060
display id: annotation2236060
version: 1
top level: BBa_I51000
location: https://synbiohub.org/public/igem/BBa_I51000/annotation2236060/range2236060/1
component: BBa_G00001

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type: Range
persistent identity: range2236060
display id: range2236060
version: 1
direction: direction/reverse
top level: BBa_I51000
start: 1
end: 21
orientation: inline

BBa_K864006
type: ComponentDefinition
persistent identity: BBa K864006
display id: BBa_K864006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB4K15(FRT) is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and kanamycin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone. The pSC101 origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
mutable provenance: Based on pSB3T5
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K864006_sequence/1

pSB4A50
type: ComponentDefinition
persistent identity: BBa K1362095
display id: BBa_K1362095
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB4A50: Low copy BioBrick cloning/expression backbone carrying Amp resistance
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1738_
m user id: 0
owner id: 22830
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
component: BBa_G00102
component: BBa_B0042
component: BBa_B0044
component: BBa_B0062
component: BBa_K1362463
component: BBa_B0043
component: BBa_B0055
component: BBa_G00000
component: BBa_I50042
component: BBa_B0054
component: BBa_G00100
component: BBa_G00001
component: BBa_P1002
component: BBa_G0003
component: BBa_B0053
component: BBa_G0002
component: BBa_G0004
sequence annotation: BBa_G00100
sequence annotation: BBa_G0003
sequence annotation: BBa_P1002
sequence annotation: BBa_B0062
sequence annotation: BBa_B0042
sequence annotation: BBa_K1362463
sequence annotation: BBa_G00102
sequence annotation: BBa_B0053
sequence annotation: BBa_B0044
sequence annotation: BBa_G00001
sequence annotation: BBa_G0004
sequence annotation: BBa_I50042
sequence annotation: BBa_B0043
sequence annotation: BBa_B0055
sequence annotation: BBa_B0054
sequence annotation: BBa_G0002
sequence annotation: BBa_G00000
sequence: https://synbiohub.org/public/igem/BBa_K1362095_sequence/1

BBa_G00102
type: Component
persistent identity: component2409781
display id: component2409781
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_G00102/1

BBa_B0042
type: Component
persistent identity: component2409776
display id: component2409776
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_B0044
type: Component
persistent identity: component2409769
display id: component2409769
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0044/1

BBa_B0062
type: Component
persistent identity: component2409783
display id: component2409783
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0062/1

BBa_K1362463
type: Component
persistent identity: component2409842
display id: component2409842
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362463/1

BBa_B0042
type: Component
persistent identity: component2409833
display id: component2409833
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_B0043
type: Component
persistent identity: component2409838
display id: component2409838
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0043/1

BBa_B0055
type: Component
persistent identity: component2409826
display id: component2409826
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0055/1

BBa_G00000
type: Component
persistent identity: component2409847
display id: component2409847
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_G00000/1

BBa_I50042
type: Component
persistent identity: component2409812
display id: component2409812
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_I50042/1

BBa_B0054
type: Component
persistent identity: component2409779
display id: component2409779
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0054/1

BBa_G00100
type: Component
persistent identity: component2409823
display id: component2409823
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_G00100/1

BBa_G00001
type: Component
persistent identity: component2409764
display id: component2409764
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_G00001/1

BBa_P1002
type: Component
persistent identity: component2409818
display id: component2409818
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_P1002/1

BBa_G0003
type: Component
persistent identity: component2409784
display id: component2409784
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_G0003/1

BBa_B0053
type: Component
persistent identity: component2409821
display id: component2409821
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0053/1

BBa_G0002
type: Component
persistent identity: component2409813
display id: component2409813
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_G0002/1

BBa_G0004
type: Component
persistent identity: component2409819
display id: component2409819
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_G0004/1

BBa_G00100
type: SequenceAnnotation
persistent identity: annotation2409823
display id: annotation2409823
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409823/range2409823/1
component: BBa_G00100

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type: Range
persistent identity: range2409823
display id: range2409823
version: 1
direction: direction/reverse
top level: pSB4A50
start: 3251
end: 3270
orientation: inline

BBa_G0003
type: SequenceAnnotation
persistent identity: annotation2409784
display id: annotation2409784
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409784/range2409784/1
component: BBa_G0003

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type: Range
persistent identity: range2409784
display id: range2409784
version: 1
direction: direction/reverse
top level: pSB4A50
start: 177
end: 183
orientation: inline

BBa_P1002
type: SequenceAnnotation
persistent identity: annotation2409818
display id: annotation2409818
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409818/range2409818/1
component: BBa_P1002

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409818/range2409818/1
type: Range
persistent identity: range2409818
display id: range2409818
version: 1
direction: direction/reverse
top level: pSB4A50
start: 2229
end: 3171
orientation: inline

BBa_B0062
type: SequenceAnnotation
persistent identity: annotation2409783
display id: annotation2409783
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409783/range2409783/1
component: BBa_B0062

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409783/range2409783/1
type: Range
persistent identity: range2409783
display id: range2409783
version: 1
direction: direction/reverse
top level: pSB4A50
start: 136
end: 176
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2409776
display id: annotation2409776
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409776/range2409776/1
component: BBa_B0042

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409776/range2409776/1
type: Range
persistent identity: range2409776
display id: range2409776
version: 1
direction: direction/reverse
top level: pSB4A50
start: 35
end: 46
orientation: inline

BBa_K1362463
type: SequenceAnnotation
persistent identity: annotation2409842
display id: annotation2409842
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409842/range2409842/1
component: BBa_K1362463

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409842/range2409842/1
type: Range
persistent identity: range2409842
display id: range2409842
version: 1
direction: direction/reverse
top level: pSB4A50
start: 3374
end: 3424
orientation: inline

BBa_G00102
type: SequenceAnnotation
persistent identity: annotation2409781
display id: annotation2409781
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409781/range2409781/1
component: BBa_G00102

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409781/range2409781/1
type: Range
persistent identity: range2409781
display id: range2409781
version: 1
direction: direction/reverse
top level: pSB4A50
start: 116
end: 135
orientation: inline

BBa_B0053
type: SequenceAnnotation
persistent identity: annotation2409821
display id: annotation2409821
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409821/range2409821/1
component: BBa_B0053

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409821/range2409821/1
type: Range
persistent identity: range2409821
display id: range2409821
version: 1
direction: direction/reverse
top level: pSB4A50
start: 3179
end: 3250
orientation: inline

BBa_B0044
type: SequenceAnnotation
persistent identity: annotation2409769
display id: annotation2409769
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409769/range2409769/1
component: BBa_B0044

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409769/range2409769/1
type: Range
persistent identity: range2409769
display id: range2409769
version: 1
direction: direction/reverse
top level: pSB4A50
start: 22
end: 34
orientation: inline

BBa_G00001
type: SequenceAnnotation
persistent identity: annotation2409764
display id: annotation2409764
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409764/range2409764/1
component: BBa_G00001

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409764/range2409764/1
type: Range
persistent identity: range2409764
display id: range2409764
version: 1
direction: direction/reverse
top level: pSB4A50
start: 1
end: 21
orientation: inline

BBa_G0004
type: SequenceAnnotation
persistent identity: annotation2409819
display id: annotation2409819
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409819/range2409819/1
component: BBa_G0004

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409819/range2409819/1
type: Range
persistent identity: range2409819
display id: range2409819
version: 1
direction: direction/reverse
top level: pSB4A50
start: 3172
end: 3178
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2409833
display id: annotation2409833
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409833/range2409833/1
component: BBa_B0042

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409833/range2409833/1
type: Range
persistent identity: range2409833
display id: range2409833
version: 1
direction: direction/reverse
top level: pSB4A50
start: 3349
end: 3360
orientation: inline

BBa_I50042
type: SequenceAnnotation
persistent identity: annotation2409812
display id: annotation2409812
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409812/range2409812/1
component: BBa_I50042

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409812/range2409812/1
type: Range
persistent identity: range2409812
display id: range2409812
version: 1
direction: direction/reverse
top level: pSB4A50
start: 184
end: 2220
orientation: inline

BBa_B0043
type: SequenceAnnotation
persistent identity: annotation2409838
display id: annotation2409838
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409838/range2409838/1
component: BBa_B0043

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409838/range2409838/1
type: Range
persistent identity: range2409838
display id: range2409838
version: 1
direction: direction/reverse
top level: pSB4A50
start: 3361
end: 3373
orientation: inline

BBa_B0055
type: SequenceAnnotation
persistent identity: annotation2409826
display id: annotation2409826
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409826/range2409826/1
component: BBa_B0055

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409826/range2409826/1
type: Range
persistent identity: range2409826
display id: range2409826
version: 1
direction: direction/reverse
top level: pSB4A50
start: 3271
end: 3348
orientation: inline

BBa_B0054
type: SequenceAnnotation
persistent identity: annotation2409779
display id: annotation2409779
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409779/range2409779/1
component: BBa_B0054

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409779/range2409779/1
type: Range
persistent identity: range2409779
display id: range2409779
version: 1
direction: direction/reverse
top level: pSB4A50
start: 47
end: 115
orientation: inline

BBa_G0002
type: SequenceAnnotation
persistent identity: annotation2409813
display id: annotation2409813
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409813/range2409813/1
component: BBa_G0002

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409813/range2409813/1
type: Range
persistent identity: range2409813
display id: range2409813
version: 1
direction: direction/reverse
top level: pSB4A50
start: 2221
end: 2228
orientation: inline

BBa_G00000
type: SequenceAnnotation
persistent identity: annotation2409847
display id: annotation2409847
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409847/range2409847/1
component: BBa_G00000

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409847/range2409847/1
type: Range
persistent identity: range2409847
display id: range2409847
version: 1
direction: direction/reverse
top level: pSB4A50
start: 3425
end: 3446
orientation: inline

pSB4A5
type: ComponentDefinition
persistent identity: pSB4A5
display id: pSB4A5
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector
creator: Reshma Shetty
created: 2007-02-26T12:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: pSB4A5 is a low copy plasmid with ampicillin resistance. It has a repA pSC101-derived replication origin. (Copy number ~10-12, Lutz and Bujard, 1997.) It is derived from the BioBricks vector scaffold BBa_I51001.
mutable notes: See http://openwetware.org/wiki/Synthetic_Biology:Vectors/pSB%2A%2A5_design
mutable provenance: BBa_I51001, pSC101 origin and a new version of ampR stripped of restriction sites.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
component: BBa_G00100
component: BBa_B0062
component: BBa_B0054
component: BBa_B0042
component: BBa_B0043
component: BBa_G00102
component: BBa_G00000
component: BBa_I50042
component: BBa_P1002
component: BBa_G00001
component: BBa_B0055
component: BBa_G0004
component: BBa_B0044
component: BBa_G0003
component: BBa_B0053
component: BBa_G0002
sequence annotation: BBa_G00102
sequence annotation: BBa_G0004
sequence annotation: BBa_G0002
sequence annotation: BBa_G00001
sequence annotation: BBa_G0003
sequence annotation: BBa_I50042
sequence annotation: BBa_B0042
sequence annotation: BBa_P1002
sequence annotation: BBa_B0054
sequence annotation: BBa_G00100
sequence annotation: BBa_B0062
sequence annotation: BBa_G00000
sequence annotation: BBa_B0055
sequence annotation: BBa_B0053
sequence annotation: BBa_B0044
sequence annotation: BBa_B0043
sequence: https://synbiohub.org/public/igem/pSB4A5_sequence/1

BBa_G00100
type: Component
persistent identity: component2236376
display id: component2236376
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00100/1

BBa_B0062
type: Component
persistent identity: component2236336
display id: component2236336
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0062/1

BBa_B0054
type: Component
persistent identity: component2236332
display id: component2236332
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0054/1

BBa_B0042
type: Component
persistent identity: component2236329
display id: component2236329
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_B0043
type: Component
persistent identity: component2236391
display id: component2236391
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0043/1

BBa_G00102
type: Component
persistent identity: component2236334
display id: component2236334
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00102/1

BBa_G00000
type: Component
persistent identity: component2236396
display id: component2236396
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00000/1

BBa_I50042
type: Component
persistent identity: component2236365
display id: component2236365
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_I50042/1

BBa_P1002
type: Component
persistent identity: component2236371
display id: component2236371
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_P1002/1

BBa_G00001
type: Component
persistent identity: component2236317
display id: component2236317
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00001/1

BBa_B0055
type: Component
persistent identity: component2236379
display id: component2236379
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0055/1

BBa_B0042
type: Component
persistent identity: component2236386
display id: component2236386
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_G0004
type: Component
persistent identity: component2236372
display id: component2236372
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0004/1

BBa_B0044
type: Component
persistent identity: component2236322
display id: component2236322
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0044/1

BBa_G0003
type: Component
persistent identity: component2236337
display id: component2236337
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0003/1

BBa_B0053
type: Component
persistent identity: component2236374
display id: component2236374
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0053/1

BBa_G0002
type: Component
persistent identity: component2236366
display id: component2236366
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0002/1

BBa_G00102
type: SequenceAnnotation
persistent identity: annotation2236334
display id: annotation2236334
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236334/range2236334/1
component: BBa_G00102

https://synbiohub.org/public/igem/pSB4A5/annotation2236334/range2236334/1
type: Range
persistent identity: range2236334
display id: range2236334
version: 1
direction: direction/reverse
top level: pSB4A5
start: 116
end: 135
orientation: inline

BBa_G0004
type: SequenceAnnotation
persistent identity: annotation2236372
display id: annotation2236372
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236372/range2236372/1
component: BBa_G0004

https://synbiohub.org/public/igem/pSB4A5/annotation2236372/range2236372/1
type: Range
persistent identity: range2236372
display id: range2236372
version: 1
direction: direction/reverse
top level: pSB4A5
start: 3172
end: 3178
orientation: inline

BBa_G0002
type: SequenceAnnotation
persistent identity: annotation2236366
display id: annotation2236366
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236366/range2236366/1
component: BBa_G0002

https://synbiohub.org/public/igem/pSB4A5/annotation2236366/range2236366/1
type: Range
persistent identity: range2236366
display id: range2236366
version: 1
direction: direction/reverse
top level: pSB4A5
start: 2221
end: 2228
orientation: inline

BBa_G00001
type: SequenceAnnotation
persistent identity: annotation2236317
display id: annotation2236317
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236317/range2236317/1
component: BBa_G00001

https://synbiohub.org/public/igem/pSB4A5/annotation2236317/range2236317/1
type: Range
persistent identity: range2236317
display id: range2236317
version: 1
direction: direction/reverse
top level: pSB4A5
start: 1
end: 21
orientation: inline

BBa_G0003
type: SequenceAnnotation
persistent identity: annotation2236337
display id: annotation2236337
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236337/range2236337/1
component: BBa_G0003

https://synbiohub.org/public/igem/pSB4A5/annotation2236337/range2236337/1
type: Range
persistent identity: range2236337
display id: range2236337
version: 1
direction: direction/reverse
top level: pSB4A5
start: 177
end: 183
orientation: inline

BBa_I50042
type: SequenceAnnotation
persistent identity: annotation2236365
display id: annotation2236365
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236365/range2236365/1
component: BBa_I50042

https://synbiohub.org/public/igem/pSB4A5/annotation2236365/range2236365/1
type: Range
persistent identity: range2236365
display id: range2236365
version: 1
direction: direction/reverse
top level: pSB4A5
start: 184
end: 2220
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2236329
display id: annotation2236329
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236329/range2236329/1
component: BBa_B0042

https://synbiohub.org/public/igem/pSB4A5/annotation2236329/range2236329/1
type: Range
persistent identity: range2236329
display id: range2236329
version: 1
direction: direction/reverse
top level: pSB4A5
start: 35
end: 46
orientation: inline

BBa_P1002
type: SequenceAnnotation
persistent identity: annotation2236371
display id: annotation2236371
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236371/range2236371/1
component: BBa_P1002

https://synbiohub.org/public/igem/pSB4A5/annotation2236371/range2236371/1
type: Range
persistent identity: range2236371
display id: range2236371
version: 1
direction: direction/reverse
top level: pSB4A5
start: 2229
end: 3171
orientation: inline

BBa_B0054
type: SequenceAnnotation
persistent identity: annotation2236332
display id: annotation2236332
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236332/range2236332/1
component: BBa_B0054

https://synbiohub.org/public/igem/pSB4A5/annotation2236332/range2236332/1
type: Range
persistent identity: range2236332
display id: range2236332
version: 1
direction: direction/reverse
top level: pSB4A5
start: 47
end: 115
orientation: inline

BBa_G00100
type: SequenceAnnotation
persistent identity: annotation2236376
display id: annotation2236376
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236376/range2236376/1
component: BBa_G00100

https://synbiohub.org/public/igem/pSB4A5/annotation2236376/range2236376/1
type: Range
persistent identity: range2236376
display id: range2236376
version: 1
direction: direction/reverse
top level: pSB4A5
start: 3251
end: 3270
orientation: inline

BBa_B0062
type: SequenceAnnotation
persistent identity: annotation2236336
display id: annotation2236336
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236336/range2236336/1
component: BBa_B0062

https://synbiohub.org/public/igem/pSB4A5/annotation2236336/range2236336/1
type: Range
persistent identity: range2236336
display id: range2236336
version: 1
direction: direction/reverse
top level: pSB4A5
start: 136
end: 176
orientation: inline

BBa_G00000
type: SequenceAnnotation
persistent identity: annotation2236396
display id: annotation2236396
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236396/range2236396/1
component: BBa_G00000

https://synbiohub.org/public/igem/pSB4A5/annotation2236396/range2236396/1
type: Range
persistent identity: range2236396
display id: range2236396
version: 1
direction: direction/reverse
top level: pSB4A5
start: 3374
end: 3395
orientation: inline

BBa_B0055
type: SequenceAnnotation
persistent identity: annotation2236379
display id: annotation2236379
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236379/range2236379/1
component: BBa_B0055

https://synbiohub.org/public/igem/pSB4A5/annotation2236379/range2236379/1
type: Range
persistent identity: range2236379
display id: range2236379
version: 1
direction: direction/reverse
top level: pSB4A5
start: 3271
end: 3348
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2236386
display id: annotation2236386
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236386/range2236386/1
component: BBa_B0042

https://synbiohub.org/public/igem/pSB4A5/annotation2236386/range2236386/1
type: Range
persistent identity: range2236386
display id: range2236386
version: 1
direction: direction/reverse
top level: pSB4A5
start: 3349
end: 3360
orientation: inline

BBa_B0053
type: SequenceAnnotation
persistent identity: annotation2236374
display id: annotation2236374
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236374/range2236374/1
component: BBa_B0053

https://synbiohub.org/public/igem/pSB4A5/annotation2236374/range2236374/1
type: Range
persistent identity: range2236374
display id: range2236374
version: 1
direction: direction/reverse
top level: pSB4A5
start: 3179
end: 3250
orientation: inline

BBa_B0044
type: SequenceAnnotation
persistent identity: annotation2236322
display id: annotation2236322
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236322/range2236322/1
component: BBa_B0044

https://synbiohub.org/public/igem/pSB4A5/annotation2236322/range2236322/1
type: Range
persistent identity: range2236322
display id: range2236322
version: 1
direction: direction/reverse
top level: pSB4A5
start: 22
end: 34
orientation: inline

BBa_B0043
type: SequenceAnnotation
persistent identity: annotation2236391
display id: annotation2236391
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236391/range2236391/1
component: BBa_B0043

https://synbiohub.org/public/igem/pSB4A5/annotation2236391/range2236391/1
type: Range
persistent identity: range2236391
display id: range2236391
version: 1
direction: direction/reverse
top level: pSB4A5
start: 3361
end: 3373
orientation: inline

pSB8A15FRT
type: ComponentDefinition
persistent identity: BBa K864020
display id: BBa_K864020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick temperature sensitive standard vector
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB8A15 is a BioBrick standard vector with low copy pSC101ts replication origin (~5 copies per cell) and ampicillin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The pSC101ts is a temperature sensitive pSC101 repelication origin and propagates at 30???C but stop replicating at 42???C. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone. The pSC101ts origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
mutable provenance: Based on BBa_K864017
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K864020_sequence/1

pSB4C50
type: ComponentDefinition
persistent identity: BBa K1362096
display id: BBa_K1362096
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB4C50: Low(?) copy BioBrick cloning/expression backbone carrying Cm resistance
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:04Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1738_
m user id: 0
owner id: 22830
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
component: BBa_G00001
component: BBa_P1004
component: BBa_B0043
component: BBa_G0003
component: BBa_G00102
component: BBa_B0055
component: BBa_B0044
component: BBa_B0062
component: BBa_G0002
component: BBa_G0004
component: BBa_B0042
component: BBa_B0053
component: BBa_K1362463
component: BBa_G00000
component: BBa_I50042
component: BBa_G00100
component: BBa_B0054
sequence annotation: BBa_B0043
sequence annotation: BBa_B0062
sequence annotation: BBa_G0002
sequence annotation: BBa_K1362463
sequence annotation: BBa_B0042
sequence annotation: BBa_G00001
sequence annotation: BBa_B0053
sequence annotation: BBa_B0055
sequence annotation: BBa_G00000
sequence annotation: BBa_G00102
sequence annotation: BBa_G0004
sequence annotation: BBa_G0003
sequence annotation: BBa_B0044
sequence annotation: BBa_P1004
sequence annotation: BBa_B0054
sequence annotation: BBa_G00100
sequence annotation: BBa_I50042
sequence: https://synbiohub.org/public/igem/BBa_K1362096_sequence/1

BBa_G00001
type: Component
persistent identity: component2409852
display id: component2409852
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_G00001/1

BBa_P1004
type: Component
persistent identity: component2409903
display id: component2409903
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_P1004/1

BBa_B0043
type: Component
persistent identity: component2409923
display id: component2409923
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0043/1

BBa_G0003
type: Component
persistent identity: component2409872
display id: component2409872
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_G0003/1

BBa_G00102
type: Component
persistent identity: component2409869
display id: component2409869
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_G00102/1

BBa_B0055
type: Component
persistent identity: component2409911
display id: component2409911
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0055/1

BBa_B0044
type: Component
persistent identity: component2409857
display id: component2409857
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0044/1

BBa_B0062
type: Component
persistent identity: component2409871
display id: component2409871
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0062/1

BBa_G0002
type: Component
persistent identity: component2409901
display id: component2409901
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_G0002/1

BBa_G0004
type: Component
persistent identity: component2409904
display id: component2409904
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_G0004/1

BBa_B0042
type: Component
persistent identity: component2409918
display id: component2409918
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_B0053
type: Component
persistent identity: component2409906
display id: component2409906
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0053/1

BBa_K1362463
type: Component
persistent identity: component2409927
display id: component2409927
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362463/1

BBa_G00000
type: Component
persistent identity: component2409932
display id: component2409932
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_G00000/1

BBa_I50042
type: Component
persistent identity: component2409900
display id: component2409900
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_I50042/1

BBa_B0042
type: Component
persistent identity: component2409864
display id: component2409864
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_G00100
type: Component
persistent identity: component2409908
display id: component2409908
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_G00100/1

BBa_B0054
type: Component
persistent identity: component2409867
display id: component2409867
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0054/1

BBa_B0043
type: SequenceAnnotation
persistent identity: annotation2409923
display id: annotation2409923
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409923/range2409923/1
component: BBa_B0043

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409923/range2409923/1
type: Range
persistent identity: range2409923
display id: range2409923
version: 1
direction: direction/reverse
top level: pSB4C50
start: 3307
end: 3319
orientation: inline

BBa_B0062
type: SequenceAnnotation
persistent identity: annotation2409871
display id: annotation2409871
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409871/range2409871/1
component: BBa_B0062

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409871/range2409871/1
type: Range
persistent identity: range2409871
display id: range2409871
version: 1
direction: direction/reverse
top level: pSB4C50
start: 176
end: 216
orientation: inline

BBa_G0002
type: SequenceAnnotation
persistent identity: annotation2409901
display id: annotation2409901
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409901/range2409901/1
component: BBa_G0002

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409901/range2409901/1
type: Range
persistent identity: range2409901
display id: range2409901
version: 1
direction: direction/reverse
top level: pSB4C50
start: 2285
end: 2292
orientation: inline

BBa_K1362463
type: SequenceAnnotation
persistent identity: annotation2409927
display id: annotation2409927
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409927/range2409927/1
component: BBa_K1362463

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409927/range2409927/1
type: Range
persistent identity: range2409927
display id: range2409927
version: 1
direction: direction/reverse
top level: pSB4C50
start: 3328
end: 3378
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2409864
display id: annotation2409864
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409864/range2409864/1
component: BBa_B0042

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409864/range2409864/1
type: Range
persistent identity: range2409864
display id: range2409864
version: 1
direction: direction/reverse
top level: pSB4C50
start: 51
end: 62
orientation: inline

BBa_G00001
type: SequenceAnnotation
persistent identity: annotation2409852
display id: annotation2409852
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409852/range2409852/1
component: BBa_G00001

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409852/range2409852/1
type: Range
persistent identity: range2409852
display id: range2409852
version: 1
direction: direction/reverse
top level: pSB4C50
start: 1
end: 21
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2409918
display id: annotation2409918
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409918/range2409918/1
component: BBa_B0042

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409918/range2409918/1
type: Range
persistent identity: range2409918
display id: range2409918
version: 1
direction: direction/reverse
top level: pSB4C50
start: 3287
end: 3298
orientation: inline

BBa_B0053
type: SequenceAnnotation
persistent identity: annotation2409906
display id: annotation2409906
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409906/range2409906/1
component: BBa_B0053

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409906/range2409906/1
type: Range
persistent identity: range2409906
display id: range2409906
version: 1
direction: direction/reverse
top level: pSB4C50
start: 3093
end: 3164
orientation: inline

BBa_B0055
type: SequenceAnnotation
persistent identity: annotation2409911
display id: annotation2409911
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409911/range2409911/1
component: BBa_B0055

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409911/range2409911/1
type: Range
persistent identity: range2409911
display id: range2409911
version: 1
direction: direction/reverse
top level: pSB4C50
start: 3201
end: 3278
orientation: inline

BBa_G00000
type: SequenceAnnotation
persistent identity: annotation2409932
display id: annotation2409932
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409932/range2409932/1
component: BBa_G00000

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409932/range2409932/1
type: Range
persistent identity: range2409932
display id: range2409932
version: 1
direction: direction/reverse
top level: pSB4C50
start: 3387
end: 3408
orientation: inline

BBa_G00102
type: SequenceAnnotation
persistent identity: annotation2409869
display id: annotation2409869
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409869/range2409869/1
component: BBa_G00102

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409869/range2409869/1
type: Range
persistent identity: range2409869
display id: range2409869
version: 1
direction: direction/reverse
top level: pSB4C50
start: 148
end: 167
orientation: inline

BBa_G0004
type: SequenceAnnotation
persistent identity: annotation2409904
display id: annotation2409904
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409904/range2409904/1
component: BBa_G0004

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409904/range2409904/1
type: Range
persistent identity: range2409904
display id: range2409904
version: 1
direction: direction/reverse
top level: pSB4C50
start: 3078
end: 3084
orientation: inline

BBa_G0003
type: SequenceAnnotation
persistent identity: annotation2409872
display id: annotation2409872
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409872/range2409872/1
component: BBa_G0003

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409872/range2409872/1
type: Range
persistent identity: range2409872
display id: range2409872
version: 1
direction: direction/reverse
top level: pSB4C50
start: 225
end: 231
orientation: inline

BBa_B0044
type: SequenceAnnotation
persistent identity: annotation2409857
display id: annotation2409857
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409857/range2409857/1
component: BBa_B0044

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409857/range2409857/1
type: Range
persistent identity: range2409857
display id: range2409857
version: 1
direction: direction/reverse
top level: pSB4C50
start: 30
end: 42
orientation: inline

BBa_P1004
type: SequenceAnnotation
persistent identity: annotation2409903
display id: annotation2409903
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409903/range2409903/1
component: BBa_P1004

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409903/range2409903/1
type: Range
persistent identity: range2409903
display id: range2409903
version: 1
direction: direction/reverse
top level: pSB4C50
start: 2301
end: 3069
orientation: inline

BBa_B0054
type: SequenceAnnotation
persistent identity: annotation2409867
display id: annotation2409867
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409867/range2409867/1
component: BBa_B0054

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409867/range2409867/1
type: Range
persistent identity: range2409867
display id: range2409867
version: 1
direction: direction/reverse
top level: pSB4C50
start: 71
end: 139
orientation: inline

BBa_G00100
type: SequenceAnnotation
persistent identity: annotation2409908
display id: annotation2409908
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409908/range2409908/1
component: BBa_G00100

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409908/range2409908/1
type: Range
persistent identity: range2409908
display id: range2409908
version: 1
direction: direction/reverse
top level: pSB4C50
start: 3173
end: 3192
orientation: inline

BBa_I50042
type: SequenceAnnotation
persistent identity: annotation2409900
display id: annotation2409900
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409900/range2409900/1
component: BBa_I50042

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409900/range2409900/1
type: Range
persistent identity: range2409900
display id: range2409900
version: 1
direction: direction/reverse
top level: pSB4C50
start: 240
end: 2276
orientation: inline

BBa_I51001
type: ComponentDefinition
persistent identity: BBa I51001
display id: BBa_I51001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BioBrick base vector, as revised by Codon Devices
creator: Reshma Shetty
created: 2007-01-29T12:00:00Z
modified: 2015-08-31T04:07:42Z
discontinued: true
dominant: false
experience: experience/Issues
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: This is a vector scaffold for constructing new BioBricks vectors. Information on the design is available at http://openwetware.org/wiki/Synthetic_Biology:Vectors/pSB%2A%2A5_design
mutable notes: Documented at http://openwetware.org/wiki/Synthetic_Biology:Vectors/pSB%2A%2A5_design Due to technical difficulties during synthesis, rather than including BBa_P1011 as designed, BBa_P1016 was synthesized as a component of this part. Hence, BBa_I51000 documents the original design and BBa_I51001 is the vector scaffold as synthesized.
mutable provenance: This is a composite part generated from several basic parts obtained from a variety of sources.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
component: BBa_B0055
component: BBa_B0044
component: BBa_B0043
component: BBa_B0045
component: BBa_B0042
component: BBa_P1006
component: BBa_G00001
component: BBa_G00100
component: BBa_G00102
component: BBa_B0054
component: BBa_G00000
component: BBa_B0053
component: BBa_P1016
component: BBa_B0062
component: BBa_I50020
sequence annotation: BBa_B0045
sequence annotation: BBa_B0043
sequence annotation: BBa_P1016
sequence annotation: BBa_B0053
sequence annotation: BBa_B0044
sequence annotation: BBa_I50020
sequence annotation: BBa_G00102
sequence annotation: BBa_G00001
sequence annotation: BBa_B0062
sequence annotation: BBa_B0042
sequence annotation: BBa_B0054
sequence annotation: BBa_G00000
sequence annotation: BBa_P1006
sequence annotation: BBa_B0055
sequence annotation: BBa_G00100
sequence: https://synbiohub.org/public/igem/BBa_I51001_sequence/1

BBa_B0055
type: Component
persistent identity: component1910726
display id: component1910726
version: 1
top level: BBa_I51001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0055/1

BBa_B0044
type: Component
persistent identity: component1910698
display id: component1910698
version: 1
top level: BBa_I51001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0044/1

BBa_B0043
type: Component
persistent identity: component1910738
display id: component1910738
version: 1
top level: BBa_I51001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0043/1

BBa_B0045
type: Component
persistent identity: component1910720
display id: component1910720
version: 1
top level: BBa_I51001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0045/1

BBa_B0042
type: Component
persistent identity: component1910733
display id: component1910733
version: 1
top level: BBa_I51001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_P1006
type: Component
persistent identity: component1910718
display id: component1910718
version: 1
top level: BBa_I51001
access: public
definition: https://synbiohub.org/public/igem/BBa_P1006/1

BBa_B0042
type: Component
persistent identity: component1910705
display id: component1910705
version: 1
top level: BBa_I51001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_G00001
type: Component
persistent identity: component1910693
display id: component1910693
version: 1
top level: BBa_I51001
access: public
definition: https://synbiohub.org/public/igem/BBa_G00001/1

BBa_G00100
type: Component
persistent identity: component1910723
display id: component1910723
version: 1
top level: BBa_I51001
access: public
definition: https://synbiohub.org/public/igem/BBa_G00100/1

BBa_B0045
type: Component
persistent identity: component1910713
display id: component1910713
version: 1
top level: BBa_I51001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0045/1

BBa_G00102
type: Component
persistent identity: component1910709
display id: component1910709
version: 1
top level: BBa_I51001
access: public
definition: https://synbiohub.org/public/igem/BBa_G00102/1

BBa_B0054
type: Component
persistent identity: component1910708
display id: component1910708
version: 1
top level: BBa_I51001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0054/1

BBa_G00000
type: Component
persistent identity: component1910740
display id: component1910740
version: 1
top level: BBa_I51001
access: public
definition: https://synbiohub.org/public/igem/BBa_G00000/1

BBa_B0053
type: Component
persistent identity: component1910722
display id: component1910722
version: 1
top level: BBa_I51001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0053/1

BBa_P1016
type: Component
persistent identity: component1910744
display id: component1910744
version: 1
top level: BBa_I51001
access: public
definition: https://synbiohub.org/public/igem/BBa_P1016/1

BBa_B0062
type: Component
persistent identity: component1910711
display id: component1910711
version: 1
top level: BBa_I51001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0062/1

BBa_I50020
type: Component
persistent identity: component1910759
display id: component1910759
version: 1
top level: BBa_I51001
access: public
definition: https://synbiohub.org/public/igem/BBa_I50020/1

BBa_B0045
type: SequenceAnnotation
persistent identity: annotation1910713
display id: annotation1910713
version: 1
top level: BBa_I51001
location: https://synbiohub.org/public/igem/BBa_I51001/annotation1910713/range1910713/1
component: BBa_B0045

https://synbiohub.org/public/igem/BBa_I51001/annotation1910713/range1910713/1
type: Range
persistent identity: range1910713
display id: range1910713
version: 1
direction: direction/reverse
top level: BBa_I51001
start: 177
end: 182
orientation: inline

BBa_B0043
type: SequenceAnnotation
persistent identity: annotation1910738
display id: annotation1910738
version: 1
top level: BBa_I51001
location: https://synbiohub.org/public/igem/BBa_I51001/annotation1910738/range1910738/1
component: BBa_B0043

https://synbiohub.org/public/igem/BBa_I51001/annotation1910738/range1910738/1
type: Range
persistent identity: range1910738
display id: range1910738
version: 1
direction: direction/reverse
top level: BBa_I51001
start: 1314
end: 1326
orientation: inline

BBa_P1016
type: SequenceAnnotation
persistent identity: annotation1910744
display id: annotation1910744
version: 1
top level: BBa_I51001
location: https://synbiohub.org/public/igem/BBa_I51001/annotation1910744/range1910744/1
component: BBa_P1016

https://synbiohub.org/public/igem/BBa_I51001/annotation1910744/range1910744/1
type: Range
persistent identity: range1910744
display id: range1910744
version: 1
direction: direction/reverse
top level: BBa_I51001
start: 1349
end: 1764
orientation: inline

BBa_B0053
type: SequenceAnnotation
persistent identity: annotation1910722
display id: annotation1910722
version: 1
top level: BBa_I51001
location: https://synbiohub.org/public/igem/BBa_I51001/annotation1910722/range1910722/1
component: BBa_B0053

https://synbiohub.org/public/igem/BBa_I51001/annotation1910722/range1910722/1
type: Range
persistent identity: range1910722
display id: range1910722
version: 1
direction: direction/reverse
top level: BBa_I51001
start: 1132
end: 1203
orientation: inline

BBa_B0044
type: SequenceAnnotation
persistent identity: annotation1910698
display id: annotation1910698
version: 1
top level: BBa_I51001
location: https://synbiohub.org/public/igem/BBa_I51001/annotation1910698/range1910698/1
component: BBa_B0044

https://synbiohub.org/public/igem/BBa_I51001/annotation1910698/range1910698/1
type: Range
persistent identity: range1910698
display id: range1910698
version: 1
direction: direction/reverse
top level: BBa_I51001
start: 22
end: 34
orientation: inline

BBa_I50020
type: SequenceAnnotation
persistent identity: annotation1910759
display id: annotation1910759
version: 1
top level: BBa_I51001
location: https://synbiohub.org/public/igem/BBa_I51001/annotation1910759/range1910759/1
component: BBa_I50020

https://synbiohub.org/public/igem/BBa_I51001/annotation1910759/range1910759/1
type: Range
persistent identity: range1910759
display id: range1910759
version: 1
direction: direction/reverse
top level: BBa_I51001
start: 1765
end: 2438
orientation: inline

BBa_G00102
type: SequenceAnnotation
persistent identity: annotation1910709
display id: annotation1910709
version: 1
top level: BBa_I51001
location: https://synbiohub.org/public/igem/BBa_I51001/annotation1910709/range1910709/1
component: BBa_G00102

https://synbiohub.org/public/igem/BBa_I51001/annotation1910709/range1910709/1
type: Range
persistent identity: range1910709
display id: range1910709
version: 1
direction: direction/reverse
top level: BBa_I51001
start: 116
end: 135
orientation: inline

BBa_G00001
type: SequenceAnnotation
persistent identity: annotation1910693
display id: annotation1910693
version: 1
top level: BBa_I51001
location: https://synbiohub.org/public/igem/BBa_I51001/annotation1910693/range1910693/1
component: BBa_G00001

https://synbiohub.org/public/igem/BBa_I51001/annotation1910693/range1910693/1
type: Range
persistent identity: range1910693
display id: range1910693
version: 1
direction: direction/reverse
top level: BBa_I51001
start: 1
end: 21
orientation: inline

BBa_B0062
type: SequenceAnnotation
persistent identity: annotation1910711
display id: annotation1910711
version: 1
top level: BBa_I51001
location: https://synbiohub.org/public/igem/BBa_I51001/annotation1910711/range1910711/1
component: BBa_B0062

https://synbiohub.org/public/igem/BBa_I51001/annotation1910711/range1910711/1
type: Range
persistent identity: range1910711
display id: range1910711
version: 1
direction: direction/reverse
top level: BBa_I51001
start: 136
end: 176
orientation: inline

BBa_B0045
type: SequenceAnnotation
persistent identity: annotation1910720
display id: annotation1910720
version: 1
top level: BBa_I51001
location: https://synbiohub.org/public/igem/BBa_I51001/annotation1910720/range1910720/1
component: BBa_B0045

https://synbiohub.org/public/igem/BBa_I51001/annotation1910720/range1910720/1
type: Range
persistent identity: range1910720
display id: range1910720
version: 1
direction: direction/reverse
top level: BBa_I51001
start: 1126
end: 1131
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation1910733
display id: annotation1910733
version: 1
top level: BBa_I51001
location: https://synbiohub.org/public/igem/BBa_I51001/annotation1910733/range1910733/1
component: BBa_B0042

https://synbiohub.org/public/igem/BBa_I51001/annotation1910733/range1910733/1
type: Range
persistent identity: range1910733
display id: range1910733
version: 1
direction: direction/reverse
top level: BBa_I51001
start: 1302
end: 1313
orientation: inline

BBa_B0054
type: SequenceAnnotation
persistent identity: annotation1910708
display id: annotation1910708
version: 1
top level: BBa_I51001
location: https://synbiohub.org/public/igem/BBa_I51001/annotation1910708/range1910708/1
component: BBa_B0054

https://synbiohub.org/public/igem/BBa_I51001/annotation1910708/range1910708/1
type: Range
persistent identity: range1910708
display id: range1910708
version: 1
direction: direction/reverse
top level: BBa_I51001
start: 47
end: 115
orientation: inline

BBa_G00000
type: SequenceAnnotation
persistent identity: annotation1910740
display id: annotation1910740
version: 1
top level: BBa_I51001
location: https://synbiohub.org/public/igem/BBa_I51001/annotation1910740/range1910740/1
component: BBa_G00000

https://synbiohub.org/public/igem/BBa_I51001/annotation1910740/range1910740/1
type: Range
persistent identity: range1910740
display id: range1910740
version: 1
direction: direction/reverse
top level: BBa_I51001
start: 1327
end: 1348
orientation: inline

BBa_P1006
type: SequenceAnnotation
persistent identity: annotation1910718
display id: annotation1910718
version: 1
top level: BBa_I51001
location: https://synbiohub.org/public/igem/BBa_I51001/annotation1910718/range1910718/1
component: BBa_P1006

https://synbiohub.org/public/igem/BBa_I51001/annotation1910718/range1910718/1
type: Range
persistent identity: range1910718
display id: range1910718
version: 1
direction: direction/reverse
top level: BBa_I51001
start: 183
end: 1125
orientation: inline

BBa_B0055
type: SequenceAnnotation
persistent identity: annotation1910726
display id: annotation1910726
version: 1
top level: BBa_I51001
location: https://synbiohub.org/public/igem/BBa_I51001/annotation1910726/range1910726/1
component: BBa_B0055

https://synbiohub.org/public/igem/BBa_I51001/annotation1910726/range1910726/1
type: Range
persistent identity: range1910726
display id: range1910726
version: 1
direction: direction/reverse
top level: BBa_I51001
start: 1224
end: 1301
orientation: inline

BBa_G00100
type: SequenceAnnotation
persistent identity: annotation1910723
display id: annotation1910723
version: 1
top level: BBa_I51001
location: https://synbiohub.org/public/igem/BBa_I51001/annotation1910723/range1910723/1
component: BBa_G00100

https://synbiohub.org/public/igem/BBa_I51001/annotation1910723/range1910723/1
type: Range
persistent identity: range1910723
display id: range1910723
version: 1
direction: direction/reverse
top level: BBa_I51001
start: 1204
end: 1223
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation1910705
display id: annotation1910705
version: 1
top level: BBa_I51001
location: https://synbiohub.org/public/igem/BBa_I51001/annotation1910705/range1910705/1
component: BBa_B0042

https://synbiohub.org/public/igem/BBa_I51001/annotation1910705/range1910705/1
type: Range
persistent identity: range1910705
display id: range1910705
version: 1
direction: direction/reverse
top level: BBa_I51001
start: 35
end: 46
orientation: inline

BBa_K864004
type: ComponentDefinition
persistent identity: BBa K864004
display id: BBa_K864004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector
creator: Erik Lundin
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB4A15 is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and ampicillin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone. The pSC101 origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
mutable provenance: based on pSB3T5
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: AmpR P1002
sequence annotation: FRT site
sequence annotation: -10
sequence annotation: RBS
sequence annotation: Lux terminator
sequence annotation: P22 late terminator
sequence annotation: TonB terminator
sequence annotation: pSC101 ori BBa_K864050
sequence annotation: Lambda Red forward primer
sequence annotation: -35
sequence annotation: VF2
sequence annotation: rrnC terminator
sequence annotation: Lambda Red reverse primer
sequence annotation: VR
sequence annotation: T3 terminator
sequence: https://synbiohub.org/public/igem/BBa_K864004_sequence/1

AmpR P1002
type: SequenceAnnotation
persistent identity: annotation2198875
display id: annotation2198875
version: 1
top level: BBa_K864004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K864004/annotation2198875/range2198875/1

https://synbiohub.org/public/igem/BBa_K864004/annotation2198875/range2198875/1
type: Range
persistent identity: range2198875
display id: range2198875
version: 1
direction: direction/reverse
top level: BBa_K864004
start: 2543
end: 3403
orientation: inline

FRT site
type: SequenceAnnotation
persistent identity: annotation2198880
display id: annotation2198880
version: 1
top level: BBa_K864004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864004/annotation2198880/range2198880/1

https://synbiohub.org/public/igem/BBa_K864004/annotation2198880/range2198880/1
type: Range
persistent identity: range2198880
display id: range2198880
version: 1
direction: direction/reverse
top level: BBa_K864004
start: 3483
end: 3516
orientation: inline

FRT site
type: SequenceAnnotation
persistent identity: annotation2198879
display id: annotation2198879
version: 1
top level: BBa_K864004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864004/annotation2198879/range2198879/1

https://synbiohub.org/public/igem/BBa_K864004/annotation2198879/range2198879/1
type: Range
persistent identity: range2198879
display id: range2198879
version: 1
direction: direction/reverse
top level: BBa_K864004
start: 2506
end: 2539
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2198877
display id: annotation2198877
version: 1
top level: BBa_K864004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K864004/annotation2198877/range2198877/1

https://synbiohub.org/public/igem/BBa_K864004/annotation2198877/range2198877/1
type: Range
persistent identity: range2198877
display id: range2198877
version: 1
direction: direction/reverse
top level: BBa_K864004
start: 3421
end: 3426
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2198876
display id: annotation2198876
version: 1
top level: BBa_K864004
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K864004/annotation2198876/range2198876/1

https://synbiohub.org/public/igem/BBa_K864004/annotation2198876/range2198876/1
type: Range
persistent identity: range2198876
display id: range2198876
version: 1
direction: direction/reverse
top level: BBa_K864004
start: 3408
end: 3412
orientation: inline

Lux terminator
type: SequenceAnnotation
persistent identity: annotation2198871
display id: annotation2198871
version: 1
top level: BBa_K864004
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864004/annotation2198871/range2198871/1

https://synbiohub.org/public/igem/BBa_K864004/annotation2198871/range2198871/1
type: Range
persistent identity: range2198871
display id: range2198871
version: 1
direction: direction/forward
top level: BBa_K864004
start: 2454
end: 2499
orientation: inline

P22 late terminator
type: SequenceAnnotation
persistent identity: annotation2198872
display id: annotation2198872
version: 1
top level: BBa_K864004
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864004/annotation2198872/range2198872/1

https://synbiohub.org/public/igem/BBa_K864004/annotation2198872/range2198872/1
type: Range
persistent identity: range2198872
display id: range2198872
version: 1
direction: direction/forward
top level: BBa_K864004
start: 3523
end: 3563
orientation: inline

TonB terminator
type: SequenceAnnotation
persistent identity: annotation2198866
display id: annotation2198866
version: 1
top level: BBa_K864004
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864004/annotation2198866/range2198866/1

https://synbiohub.org/public/igem/BBa_K864004/annotation2198866/range2198866/1
type: Range
persistent identity: range2198866
display id: range2198866
version: 1
direction: direction/forward
top level: BBa_K864004
start: 47
end: 115
orientation: inline

pSC101 ori BBa_K864050
type: SequenceAnnotation
persistent identity: annotation2198869
display id: annotation2198869
version: 1
top level: BBa_K864004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864004/annotation2198869/range2198869/1

https://synbiohub.org/public/igem/BBa_K864004/annotation2198869/range2198869/1
type: Range
persistent identity: range2198869
display id: range2198869
version: 1
direction: direction/forward
top level: BBa_K864004
start: 190
end: 2405
orientation: inline

Lambda Red forward primer
type: SequenceAnnotation
persistent identity: annotation2198870
display id: annotation2198870
version: 1
top level: BBa_K864004
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864004/annotation2198870/range2198870/1

https://synbiohub.org/public/igem/BBa_K864004/annotation2198870/range2198870/1
type: Range
persistent identity: range2198870
display id: range2198870
version: 1
direction: direction/forward
top level: BBa_K864004
start: 2433
end: 2450
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2198878
display id: annotation2198878
version: 1
top level: BBa_K864004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K864004/annotation2198878/range2198878/1

https://synbiohub.org/public/igem/BBa_K864004/annotation2198878/range2198878/1
type: Range
persistent identity: range2198878
display id: range2198878
version: 1
direction: direction/forward
top level: BBa_K864004
start: 3447
end: 3452
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation2198873
display id: annotation2198873
version: 1
top level: BBa_K864004
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864004/annotation2198873/range2198873/1

https://synbiohub.org/public/igem/BBa_K864004/annotation2198873/range2198873/1
type: Range
persistent identity: range2198873
display id: range2198873
version: 1
direction: direction/forward
top level: BBa_K864004
start: 3564
end: 3583
orientation: inline

rrnC terminator
type: SequenceAnnotation
persistent identity: annotation2198868
display id: annotation2198868
version: 1
top level: BBa_K864004
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864004/annotation2198868/range2198868/1

https://synbiohub.org/public/igem/BBa_K864004/annotation2198868/range2198868/1
type: Range
persistent identity: range2198868
display id: range2198868
version: 1
direction: direction/forward
top level: BBa_K864004
start: 136
end: 175
orientation: inline

Lambda Red reverse primer
type: SequenceAnnotation
persistent identity: annotation2198865
display id: annotation2198865
version: 1
top level: BBa_K864004
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864004/annotation2198865/range2198865/1

https://synbiohub.org/public/igem/BBa_K864004/annotation2198865/range2198865/1
type: Range
persistent identity: range2198865
display id: range2198865
version: 1
direction: direction/reverse
top level: BBa_K864004
start: 18
end: 38
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation2198867
display id: annotation2198867
version: 1
top level: BBa_K864004
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864004/annotation2198867/range2198867/1

https://synbiohub.org/public/igem/BBa_K864004/annotation2198867/range2198867/1
type: Range
persistent identity: range2198867
display id: range2198867
version: 1
direction: direction/forward
top level: BBa_K864004
start: 116
end: 135
orientation: inline

T3 terminator
type: SequenceAnnotation
persistent identity: annotation2198874
display id: annotation2198874
version: 1
top level: BBa_K864004
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864004/annotation2198874/range2198874/1

https://synbiohub.org/public/igem/BBa_K864004/annotation2198874/range2198874/1
type: Range
persistent identity: range2198874
display id: range2198874
version: 1
direction: direction/forward
top level: BBa_K864004
start: 3584
end: 3661
orientation: inline

pSB4K50
type: ComponentDefinition
persistent identity: BBa K1362097
display id: BBa_K1362097
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick expression backbone carrying Kan resistance
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1738_
m user id: 0
owner id: 22830
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
component: BBa_B0044
component: BBa_B0042
component: BBa_I50041
component: BBa_K1362463
component: BBa_G00001
component: BBa_G00100
component: BBa_G00102
component: BBa_B0054
component: BBa_B0062
component: BBa_G0001
component: BBa_B0043
component: BBa_B0055
component: BBa_G00000
component: BBa_P1007
component: BBa_G0005
component: BBa_G0006
component: BBa_B0053
sequence annotation: BBa_B0044
sequence annotation: BBa_B0042
sequence annotation: BBa_G0005
sequence annotation: BBa_I50041
sequence annotation: BBa_B0055
sequence annotation: BBa_B0062
sequence annotation: BBa_G00001
sequence annotation: BBa_G00102
sequence annotation: BBa_G0006
sequence annotation: BBa_G00100
sequence annotation: BBa_K1362463
sequence annotation: BBa_B0053
sequence annotation: BBa_G00000
sequence annotation: BBa_B0043
sequence annotation: BBa_B0054
sequence annotation: BBa_P1007
sequence annotation: BBa_G0001
sequence: https://synbiohub.org/public/igem/BBa_K1362097_sequence/1

BBa_B0044
type: Component
persistent identity: component2409942
display id: component2409942
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0044/1

BBa_B0042
type: Component
persistent identity: component2410003
display id: component2410003
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_I50041
type: Component
persistent identity: component2409988
display id: component2409988
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_I50041/1

BBa_K1362463
type: Component
persistent identity: component2410012
display id: component2410012
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362463/1

BBa_G00001
type: Component
persistent identity: component2409937
display id: component2409937
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_G00001/1

BBa_G00100
type: Component
persistent identity: component2409993
display id: component2409993
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_G00100/1

BBa_G00102
type: Component
persistent identity: component2409954
display id: component2409954
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_G00102/1

BBa_B0054
type: Component
persistent identity: component2409952
display id: component2409952
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0054/1

BBa_B0062
type: Component
persistent identity: component2409956
display id: component2409956
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0062/1

BBa_G0001
type: Component
persistent identity: component2409962
display id: component2409962
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_G0001/1

BBa_B0043
type: Component
persistent identity: component2410008
display id: component2410008
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0043/1

BBa_B0055
type: Component
persistent identity: component2409996
display id: component2409996
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0055/1

BBa_G00000
type: Component
persistent identity: component2410017
display id: component2410017
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_G00000/1

BBa_P1007
type: Component
persistent identity: component2409961
display id: component2409961
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_P1007/1

BBa_B0042
type: Component
persistent identity: component2409949
display id: component2409949
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_G0005
type: Component
persistent identity: component2409957
display id: component2409957
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_G0005/1

BBa_G0006
type: Component
persistent identity: component2409989
display id: component2409989
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_G0006/1

BBa_B0053
type: Component
persistent identity: component2409991
display id: component2409991
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0053/1

BBa_B0044
type: SequenceAnnotation
persistent identity: annotation2409942
display id: annotation2409942
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2409942/range2409942/1
component: BBa_B0044

https://synbiohub.org/public/igem/BBa_K1362097/annotation2409942/range2409942/1
type: Range
persistent identity: range2409942
display id: range2409942
version: 1
direction: direction/reverse
top level: pSB4K50
start: 22
end: 34
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2410003
display id: annotation2410003
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2410003/range2410003/1
component: BBa_B0042

https://synbiohub.org/public/igem/BBa_K1362097/annotation2410003/range2410003/1
type: Range
persistent identity: range2410003
display id: range2410003
version: 1
direction: direction/reverse
top level: pSB4K50
start: 3373
end: 3384
orientation: inline

BBa_G0005
type: SequenceAnnotation
persistent identity: annotation2409957
display id: annotation2409957
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2409957/range2409957/1
component: BBa_G0005

https://synbiohub.org/public/igem/BBa_K1362097/annotation2409957/range2409957/1
type: Range
persistent identity: range2409957
display id: range2409957
version: 1
direction: direction/reverse
top level: pSB4K50
start: 177
end: 183
orientation: inline

BBa_I50041
type: SequenceAnnotation
persistent identity: annotation2409988
display id: annotation2409988
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2409988/range2409988/1
component: BBa_I50041

https://synbiohub.org/public/igem/BBa_K1362097/annotation2409988/range2409988/1
type: Range
persistent identity: range2409988
display id: range2409988
version: 1
direction: direction/reverse
top level: pSB4K50
start: 1159
end: 3195
orientation: inline

BBa_B0055
type: SequenceAnnotation
persistent identity: annotation2409996
display id: annotation2409996
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2409996/range2409996/1
component: BBa_B0055

https://synbiohub.org/public/igem/BBa_K1362097/annotation2409996/range2409996/1
type: Range
persistent identity: range2409996
display id: range2409996
version: 1
direction: direction/reverse
top level: pSB4K50
start: 3295
end: 3372
orientation: inline

BBa_B0062
type: SequenceAnnotation
persistent identity: annotation2409956
display id: annotation2409956
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2409956/range2409956/1
component: BBa_B0062

https://synbiohub.org/public/igem/BBa_K1362097/annotation2409956/range2409956/1
type: Range
persistent identity: range2409956
display id: range2409956
version: 1
direction: direction/reverse
top level: pSB4K50
start: 136
end: 176
orientation: inline

BBa_G00001
type: SequenceAnnotation
persistent identity: annotation2409937
display id: annotation2409937
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2409937/range2409937/1
component: BBa_G00001

https://synbiohub.org/public/igem/BBa_K1362097/annotation2409937/range2409937/1
type: Range
persistent identity: range2409937
display id: range2409937
version: 1
direction: direction/reverse
top level: pSB4K50
start: 1
end: 21
orientation: inline

BBa_G00102
type: SequenceAnnotation
persistent identity: annotation2409954
display id: annotation2409954
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2409954/range2409954/1
component: BBa_G00102

https://synbiohub.org/public/igem/BBa_K1362097/annotation2409954/range2409954/1
type: Range
persistent identity: range2409954
display id: range2409954
version: 1
direction: direction/reverse
top level: pSB4K50
start: 116
end: 135
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2409949
display id: annotation2409949
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2409949/range2409949/1
component: BBa_B0042

https://synbiohub.org/public/igem/BBa_K1362097/annotation2409949/range2409949/1
type: Range
persistent identity: range2409949
display id: range2409949
version: 1
direction: direction/reverse
top level: pSB4K50
start: 35
end: 46
orientation: inline

BBa_G0006
type: SequenceAnnotation
persistent identity: annotation2409989
display id: annotation2409989
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2409989/range2409989/1
component: BBa_G0006

https://synbiohub.org/public/igem/BBa_K1362097/annotation2409989/range2409989/1
type: Range
persistent identity: range2409989
display id: range2409989
version: 1
direction: direction/reverse
top level: pSB4K50
start: 3196
end: 3202
orientation: inline

BBa_G00100
type: SequenceAnnotation
persistent identity: annotation2409993
display id: annotation2409993
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2409993/range2409993/1
component: BBa_G00100

https://synbiohub.org/public/igem/BBa_K1362097/annotation2409993/range2409993/1
type: Range
persistent identity: range2409993
display id: range2409993
version: 1
direction: direction/reverse
top level: pSB4K50
start: 3275
end: 3294
orientation: inline

BBa_K1362463
type: SequenceAnnotation
persistent identity: annotation2410012
display id: annotation2410012
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2410012/range2410012/1
component: BBa_K1362463

https://synbiohub.org/public/igem/BBa_K1362097/annotation2410012/range2410012/1
type: Range
persistent identity: range2410012
display id: range2410012
version: 1
direction: direction/reverse
top level: pSB4K50
start: 3398
end: 3448
orientation: inline

BBa_B0053
type: SequenceAnnotation
persistent identity: annotation2409991
display id: annotation2409991
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2409991/range2409991/1
component: BBa_B0053

https://synbiohub.org/public/igem/BBa_K1362097/annotation2409991/range2409991/1
type: Range
persistent identity: range2409991
display id: range2409991
version: 1
direction: direction/reverse
top level: pSB4K50
start: 3203
end: 3274
orientation: inline

BBa_G00000
type: SequenceAnnotation
persistent identity: annotation2410017
display id: annotation2410017
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2410017/range2410017/1
component: BBa_G00000

https://synbiohub.org/public/igem/BBa_K1362097/annotation2410017/range2410017/1
type: Range
persistent identity: range2410017
display id: range2410017
version: 1
direction: direction/reverse
top level: pSB4K50
start: 3449
end: 3470
orientation: inline

BBa_B0043
type: SequenceAnnotation
persistent identity: annotation2410008
display id: annotation2410008
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2410008/range2410008/1
component: BBa_B0043

https://synbiohub.org/public/igem/BBa_K1362097/annotation2410008/range2410008/1
type: Range
persistent identity: range2410008
display id: range2410008
version: 1
direction: direction/reverse
top level: pSB4K50
start: 3385
end: 3397
orientation: inline

BBa_B0054
type: SequenceAnnotation
persistent identity: annotation2409952
display id: annotation2409952
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2409952/range2409952/1
component: BBa_B0054

https://synbiohub.org/public/igem/BBa_K1362097/annotation2409952/range2409952/1
type: Range
persistent identity: range2409952
display id: range2409952
version: 1
direction: direction/reverse
top level: pSB4K50
start: 47
end: 115
orientation: inline

BBa_P1007
type: SequenceAnnotation
persistent identity: annotation2409961
display id: annotation2409961
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2409961/range2409961/1
component: BBa_P1007

https://synbiohub.org/public/igem/BBa_K1362097/annotation2409961/range2409961/1
type: Range
persistent identity: range2409961
display id: range2409961
version: 1
direction: direction/reverse
top level: pSB4K50
start: 184
end: 1150
orientation: inline

BBa_G0001
type: SequenceAnnotation
persistent identity: annotation2409962
display id: annotation2409962
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2409962/range2409962/1
component: BBa_G0001

https://synbiohub.org/public/igem/BBa_K1362097/annotation2409962/range2409962/1
type: Range
persistent identity: range2409962
display id: range2409962
version: 1
direction: direction/reverse
top level: pSB4K50
start: 1151
end: 1158
orientation: inline

pSB4C5
type: ComponentDefinition
persistent identity: pSB4C5
display id: pSB4C5
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector
creator: Reshma Shetty
created: 2007-02-26T12:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: pSB4C3 is a low copy plasmid based with chloramphenicol resistance. It has a repA pSC101-derived replication origin. (Copy number ~10-12, Lutz and Bujard, 1997.) pSB4C5 is derived from the BioBricks vector scaffold BBa_I51001. However, the direction of the resistance marker and replication origin is not optimal.
mutable notes: None.
mutable provenance: BBa_I51001 BioBricks vector scaffold, pSC101 based origin and a version of the chloramphenicol rsistance cassette that has been cleaned of restriction sites.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
component: BBa_G00001
component: BBa_G0002
component: BBa_G0003
component: BBa_I50042
component: BBa_B0055
component: BBa_B0043
component: BBa_B0054
component: BBa_P1004
component: BBa_B0062
component: BBa_B0042
component: BBa_G00100
component: BBa_G00102
component: BBa_G0004
component: BBa_B0044
component: BBa_G00000
component: BBa_B0053
sequence annotation: BBa_B0044
sequence annotation: BBa_B0042
sequence annotation: BBa_G00100
sequence annotation: BBa_G0004
sequence annotation: BBa_G00001
sequence annotation: BBa_G0002
sequence annotation: BBa_P1004
sequence annotation: BBa_B0055
sequence annotation: BBa_G00102
sequence annotation: BBa_B0054
sequence annotation: BBa_B0043
sequence annotation: BBa_I50042
sequence annotation: BBa_G00000
sequence annotation: BBa_G0003
sequence annotation: BBa_B0062
sequence annotation: BBa_B0053
sequence: https://synbiohub.org/public/igem/pSB4C5_sequence/1

BBa_G00001
type: Component
persistent identity: component2236236
display id: component2236236
version: 1
top level: pSB4C5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00001/1

BBa_G0002
type: Component
persistent identity: component2236285
display id: component2236285
version: 1
top level: pSB4C5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0002/1

BBa_G0003
type: Component
persistent identity: component2236256
display id: component2236256
version: 1
top level: pSB4C5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0003/1

BBa_I50042
type: Component
persistent identity: component2236284
display id: component2236284
version: 1
top level: pSB4C5
access: public
definition: https://synbiohub.org/public/igem/BBa_I50042/1

BBa_B0055
type: Component
persistent identity: component2236295
display id: component2236295
version: 1
top level: pSB4C5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0055/1

BBa_B0043
type: Component
persistent identity: component2236307
display id: component2236307
version: 1
top level: pSB4C5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0043/1

BBa_B0054
type: Component
persistent identity: component2236251
display id: component2236251
version: 1
top level: pSB4C5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0054/1

BBa_P1004
type: Component
persistent identity: component2236287
display id: component2236287
version: 1
top level: pSB4C5
access: public
definition: https://synbiohub.org/public/igem/BBa_P1004/1

BBa_B0062
type: Component
persistent identity: component2236255
display id: component2236255
version: 1
top level: pSB4C5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0062/1

BBa_B0042
type: Component
persistent identity: component2236248
display id: component2236248
version: 1
top level: pSB4C5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_G00100
type: Component
persistent identity: component2236292
display id: component2236292
version: 1
top level: pSB4C5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00100/1

BBa_G00102
type: Component
persistent identity: component2236253
display id: component2236253
version: 1
top level: pSB4C5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00102/1

BBa_G0004
type: Component
persistent identity: component2236288
display id: component2236288
version: 1
top level: pSB4C5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0004/1

BBa_B0042
type: Component
persistent identity: component2236302
display id: component2236302
version: 1
top level: pSB4C5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_B0044
type: Component
persistent identity: component2236241
display id: component2236241
version: 1
top level: pSB4C5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0044/1

BBa_G00000
type: Component
persistent identity: component2236312
display id: component2236312
version: 1
top level: pSB4C5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00000/1

BBa_B0053
type: Component
persistent identity: component2236290
display id: component2236290
version: 1
top level: pSB4C5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0053/1

BBa_B0044
type: SequenceAnnotation
persistent identity: annotation2236241
display id: annotation2236241
version: 1
top level: pSB4C5
location: https://synbiohub.org/public/igem/pSB4C5/annotation2236241/range2236241/1
component: BBa_B0044

https://synbiohub.org/public/igem/pSB4C5/annotation2236241/range2236241/1
type: Range
persistent identity: range2236241
display id: range2236241
version: 1
direction: direction/reverse
top level: pSB4C5
start: 22
end: 34
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2236302
display id: annotation2236302
version: 1
top level: pSB4C5
location: https://synbiohub.org/public/igem/pSB4C5/annotation2236302/range2236302/1
component: BBa_B0042

https://synbiohub.org/public/igem/pSB4C5/annotation2236302/range2236302/1
type: Range
persistent identity: range2236302
display id: range2236302
version: 1
direction: direction/reverse
top level: pSB4C5
start: 3175
end: 3186
orientation: inline

BBa_G00100
type: SequenceAnnotation
persistent identity: annotation2236292
display id: annotation2236292
version: 1
top level: pSB4C5
location: https://synbiohub.org/public/igem/pSB4C5/annotation2236292/range2236292/1
component: BBa_G00100

https://synbiohub.org/public/igem/pSB4C5/annotation2236292/range2236292/1
type: Range
persistent identity: range2236292
display id: range2236292
version: 1
direction: direction/reverse
top level: pSB4C5
start: 3077
end: 3096
orientation: inline

BBa_G0004
type: SequenceAnnotation
persistent identity: annotation2236288
display id: annotation2236288
version: 1
top level: pSB4C5
location: https://synbiohub.org/public/igem/pSB4C5/annotation2236288/range2236288/1
component: BBa_G0004

https://synbiohub.org/public/igem/pSB4C5/annotation2236288/range2236288/1
type: Range
persistent identity: range2236288
display id: range2236288
version: 1
direction: direction/reverse
top level: pSB4C5
start: 2998
end: 3004
orientation: inline

BBa_G00001
type: SequenceAnnotation
persistent identity: annotation2236236
display id: annotation2236236
version: 1
top level: pSB4C5
location: https://synbiohub.org/public/igem/pSB4C5/annotation2236236/range2236236/1
component: BBa_G00001

https://synbiohub.org/public/igem/pSB4C5/annotation2236236/range2236236/1
type: Range
persistent identity: range2236236
display id: range2236236
version: 1
direction: direction/reverse
top level: pSB4C5
start: 1
end: 21
orientation: inline

BBa_G0002
type: SequenceAnnotation
persistent identity: annotation2236285
display id: annotation2236285
version: 1
top level: pSB4C5
location: https://synbiohub.org/public/igem/pSB4C5/annotation2236285/range2236285/1
component: BBa_G0002

https://synbiohub.org/public/igem/pSB4C5/annotation2236285/range2236285/1
type: Range
persistent identity: range2236285
display id: range2236285
version: 1
direction: direction/reverse
top level: pSB4C5
start: 2221
end: 2228
orientation: inline

BBa_P1004
type: SequenceAnnotation
persistent identity: annotation2236287
display id: annotation2236287
version: 1
top level: pSB4C5
location: https://synbiohub.org/public/igem/pSB4C5/annotation2236287/range2236287/1
component: BBa_P1004

https://synbiohub.org/public/igem/pSB4C5/annotation2236287/range2236287/1
type: Range
persistent identity: range2236287
display id: range2236287
version: 1
direction: direction/reverse
top level: pSB4C5
start: 2229
end: 2997
orientation: inline

BBa_B0055
type: SequenceAnnotation
persistent identity: annotation2236295
display id: annotation2236295
version: 1
top level: pSB4C5
location: https://synbiohub.org/public/igem/pSB4C5/annotation2236295/range2236295/1
component: BBa_B0055

https://synbiohub.org/public/igem/pSB4C5/annotation2236295/range2236295/1
type: Range
persistent identity: range2236295
display id: range2236295
version: 1
direction: direction/reverse
top level: pSB4C5
start: 3097
end: 3174
orientation: inline

BBa_G00102
type: SequenceAnnotation
persistent identity: annotation2236253
display id: annotation2236253
version: 1
top level: pSB4C5
location: https://synbiohub.org/public/igem/pSB4C5/annotation2236253/range2236253/1
component: BBa_G00102

https://synbiohub.org/public/igem/pSB4C5/annotation2236253/range2236253/1
type: Range
persistent identity: range2236253
display id: range2236253
version: 1
direction: direction/reverse
top level: pSB4C5
start: 116
end: 135
orientation: inline

BBa_B0054
type: SequenceAnnotation
persistent identity: annotation2236251
display id: annotation2236251
version: 1
top level: pSB4C5
location: https://synbiohub.org/public/igem/pSB4C5/annotation2236251/range2236251/1
component: BBa_B0054

https://synbiohub.org/public/igem/pSB4C5/annotation2236251/range2236251/1
type: Range
persistent identity: range2236251
display id: range2236251
version: 1
direction: direction/reverse
top level: pSB4C5
start: 47
end: 115
orientation: inline

BBa_B0043
type: SequenceAnnotation
persistent identity: annotation2236307
display id: annotation2236307
version: 1
top level: pSB4C5
location: https://synbiohub.org/public/igem/pSB4C5/annotation2236307/range2236307/1
component: BBa_B0043

https://synbiohub.org/public/igem/pSB4C5/annotation2236307/range2236307/1
type: Range
persistent identity: range2236307
display id: range2236307
version: 1
direction: direction/reverse
top level: pSB4C5
start: 3187
end: 3199
orientation: inline

BBa_I50042
type: SequenceAnnotation
persistent identity: annotation2236284
display id: annotation2236284
version: 1
top level: pSB4C5
location: https://synbiohub.org/public/igem/pSB4C5/annotation2236284/range2236284/1
component: BBa_I50042

https://synbiohub.org/public/igem/pSB4C5/annotation2236284/range2236284/1
type: Range
persistent identity: range2236284
display id: range2236284
version: 1
direction: direction/reverse
top level: pSB4C5
start: 184
end: 2220
orientation: inline

BBa_G00000
type: SequenceAnnotation
persistent identity: annotation2236312
display id: annotation2236312
version: 1
top level: pSB4C5
location: https://synbiohub.org/public/igem/pSB4C5/annotation2236312/range2236312/1
component: BBa_G00000

https://synbiohub.org/public/igem/pSB4C5/annotation2236312/range2236312/1
type: Range
persistent identity: range2236312
display id: range2236312
version: 1
direction: direction/reverse
top level: pSB4C5
start: 3200
end: 3221
orientation: inline

BBa_G0003
type: SequenceAnnotation
persistent identity: annotation2236256
display id: annotation2236256
version: 1
top level: pSB4C5
location: https://synbiohub.org/public/igem/pSB4C5/annotation2236256/range2236256/1
component: BBa_G0003

https://synbiohub.org/public/igem/pSB4C5/annotation2236256/range2236256/1
type: Range
persistent identity: range2236256
display id: range2236256
version: 1
direction: direction/reverse
top level: pSB4C5
start: 177
end: 183
orientation: inline

BBa_B0062
type: SequenceAnnotation
persistent identity: annotation2236255
display id: annotation2236255
version: 1
top level: pSB4C5
location: https://synbiohub.org/public/igem/pSB4C5/annotation2236255/range2236255/1
component: BBa_B0062

https://synbiohub.org/public/igem/pSB4C5/annotation2236255/range2236255/1
type: Range
persistent identity: range2236255
display id: range2236255
version: 1
direction: direction/reverse
top level: pSB4C5
start: 136
end: 176
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2236248
display id: annotation2236248
version: 1
top level: pSB4C5
location: https://synbiohub.org/public/igem/pSB4C5/annotation2236248/range2236248/1
component: BBa_B0042

https://synbiohub.org/public/igem/pSB4C5/annotation2236248/range2236248/1
type: Range
persistent identity: range2236248
display id: range2236248
version: 1
direction: direction/reverse
top level: pSB4C5
start: 35
end: 46
orientation: inline

BBa_B0053
type: SequenceAnnotation
persistent identity: annotation2236290
display id: annotation2236290
version: 1
top level: pSB4C5
location: https://synbiohub.org/public/igem/pSB4C5/annotation2236290/range2236290/1
component: BBa_B0053

https://synbiohub.org/public/igem/pSB4C5/annotation2236290/range2236290/1
type: Range
persistent identity: range2236290
display id: range2236290
version: 1
direction: direction/reverse
top level: pSB4C5
start: 3005
end: 3076
orientation: inline

pSB8C15FRT
type: ComponentDefinition
persistent identity: BBa K864021
display id: BBa_K864021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick temperature sensitive standard vector
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB8A15 is a BioBrick standard vector with low copy pSC101ts replication origin (~5 copies per cell) and ampicillin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The pSC101ts is a temperature sensitive pSC101 repelication origin and propagates at 30???C but stop replicating at 42???C. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone. The pSC101ts origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
mutable provenance: Based on BBa_K864017
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K864021_sequence/1

BBa_K864008
type: ComponentDefinition
persistent identity: BBa K864008
display id: BBa_K864008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector with LacI
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB4A15 is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and ampicillin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22. This vector carries a LacIq cassette on the backbone for tight repression of any lac promoter cloned into the cloning site.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone. The pSC101 origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
mutable provenance: Based on BBa_K864000
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K864008_sequence/1

BBa_K864013
type: ComponentDefinition
persistent identity: BBa K864013
display id: BBa_K864013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector with LacI
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB4C15Iq(FRT) is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and chloramphenicol antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22. This vector carries a LacIq cassette on the backbone for tight repression of any lac promoter cloned into the cloning site.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone.
mutable provenance: Based on BBa_K864005
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K864013_sequence/1

pSB8K15FRT
type: ComponentDefinition
persistent identity: BBa K864022
display id: BBa_K864022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick temperature sensitive standard vector
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: awd
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone. The pSC101ts origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
mutable provenance: Based on BBa_K864017
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K864022_sequence/1

pSB3K5
type: ComponentDefinition
persistent identity: pSB3K5
display id: pSB3K5
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low to medium copy BioBrick standard vector
creator: Reshma Shetty
created: 2007-05-02T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: pSB3K5 is a low to medium copy plasmid based with kanamycin resistance. It has a p15A-derived replication origin. pSB3K5 is derived from the BioBricks vector scaffold BBa_I51001.
mutable notes: None.
mutable provenance: BBa_I51001 BioBricks vector scaffold, p15A based origin and a version of the kanamycin resistance cassette that has been cleaned of restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
component: BBa_B0043
component: BBa_B0055
component: BBa_G0004
component: BBa_G00100
component: BBa_B0044
component: BBa_P1003
component: BBa_G00001
component: BBa_G00102
component: BBa_B0042
component: BBa_G0002
component: BBa_B0062
component: BBa_I50032
component: BBa_G00000
component: BBa_G0003
component: BBa_B0054
component: BBa_B0053
sequence annotation: BBa_B0062
sequence annotation: BBa_G00102
sequence annotation: BBa_G00000
sequence annotation: BBa_B0054
sequence annotation: BBa_B0044
sequence annotation: BBa_B0043
sequence annotation: BBa_G00100
sequence annotation: BBa_B0042
sequence annotation: BBa_B0055
sequence annotation: BBa_G0003
sequence annotation: BBa_P1003
sequence annotation: BBa_G00001
sequence annotation: BBa_G0002
sequence annotation: BBa_B0053
sequence annotation: BBa_G0004
sequence annotation: BBa_I50032
sequence: https://synbiohub.org/public/igem/pSB3K5_sequence/1

BBa_B0043
type: Component
persistent identity: component1932122
display id: component1932122
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0043/1

BBa_B0055
type: Component
persistent identity: component1932110
display id: component1932110
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0055/1

BBa_G0004
type: Component
persistent identity: component1932104
display id: component1932104
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0004/1

BBa_G00100
type: Component
persistent identity: component1932107
display id: component1932107
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00100/1

BBa_B0044
type: Component
persistent identity: component1932083
display id: component1932083
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0044/1

BBa_P1003
type: Component
persistent identity: component1932103
display id: component1932103
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_P1003/1

BBa_G00001
type: Component
persistent identity: component1932078
display id: component1932078
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00001/1

BBa_G00102
type: Component
persistent identity: component1932094
display id: component1932094
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00102/1

BBa_B0042
type: Component
persistent identity: component1932117
display id: component1932117
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_G0002
type: Component
persistent identity: component1932101
display id: component1932101
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0002/1

BBa_B0062
type: Component
persistent identity: component1932096
display id: component1932096
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0062/1

BBa_I50032
type: Component
persistent identity: component1932100
display id: component1932100
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_I50032/1

BBa_G00000
type: Component
persistent identity: component1932124
display id: component1932124
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00000/1

BBa_G0003
type: Component
persistent identity: component1932097
display id: component1932097
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0003/1

BBa_B0054
type: Component
persistent identity: component1932093
display id: component1932093
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0054/1

BBa_B0053
type: Component
persistent identity: component1932106
display id: component1932106
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0053/1

BBa_B0042
type: Component
persistent identity: component1932090
display id: component1932090
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_B0062
type: SequenceAnnotation
persistent identity: annotation1932096
display id: annotation1932096
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932096/range1932096/1
component: BBa_B0062

https://synbiohub.org/public/igem/pSB3K5/annotation1932096/range1932096/1
type: Range
persistent identity: range1932096
display id: range1932096
version: 1
direction: direction/reverse
top level: pSB3K5
start: 136
end: 176
orientation: inline

BBa_G00102
type: SequenceAnnotation
persistent identity: annotation1932094
display id: annotation1932094
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932094/range1932094/1
component: BBa_G00102

https://synbiohub.org/public/igem/pSB3K5/annotation1932094/range1932094/1
type: Range
persistent identity: range1932094
display id: range1932094
version: 1
direction: direction/reverse
top level: pSB3K5
start: 116
end: 135
orientation: inline

BBa_G00000
type: SequenceAnnotation
persistent identity: annotation1932124
display id: annotation1932124
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932124/range1932124/1
component: BBa_G00000

https://synbiohub.org/public/igem/pSB3K5/annotation1932124/range1932124/1
type: Range
persistent identity: range1932124
display id: range1932124
version: 1
direction: direction/reverse
top level: pSB3K5
start: 2915
end: 2936
orientation: inline

BBa_B0054
type: SequenceAnnotation
persistent identity: annotation1932093
display id: annotation1932093
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932093/range1932093/1
component: BBa_B0054

https://synbiohub.org/public/igem/pSB3K5/annotation1932093/range1932093/1
type: Range
persistent identity: range1932093
display id: range1932093
version: 1
direction: direction/reverse
top level: pSB3K5
start: 47
end: 115
orientation: inline

BBa_B0044
type: SequenceAnnotation
persistent identity: annotation1932083
display id: annotation1932083
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932083/range1932083/1
component: BBa_B0044

https://synbiohub.org/public/igem/pSB3K5/annotation1932083/range1932083/1
type: Range
persistent identity: range1932083
display id: range1932083
version: 1
direction: direction/reverse
top level: pSB3K5
start: 22
end: 34
orientation: inline

BBa_B0043
type: SequenceAnnotation
persistent identity: annotation1932122
display id: annotation1932122
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932122/range1932122/1
component: BBa_B0043

https://synbiohub.org/public/igem/pSB3K5/annotation1932122/range1932122/1
type: Range
persistent identity: range1932122
display id: range1932122
version: 1
direction: direction/reverse
top level: pSB3K5
start: 2902
end: 2914
orientation: inline

BBa_G00100
type: SequenceAnnotation
persistent identity: annotation1932107
display id: annotation1932107
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932107/range1932107/1
component: BBa_G00100

https://synbiohub.org/public/igem/pSB3K5/annotation1932107/range1932107/1
type: Range
persistent identity: range1932107
display id: range1932107
version: 1
direction: direction/reverse
top level: pSB3K5
start: 2792
end: 2811
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation1932090
display id: annotation1932090
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932090/range1932090/1
component: BBa_B0042

https://synbiohub.org/public/igem/pSB3K5/annotation1932090/range1932090/1
type: Range
persistent identity: range1932090
display id: range1932090
version: 1
direction: direction/reverse
top level: pSB3K5
start: 35
end: 46
orientation: inline

BBa_B0055
type: SequenceAnnotation
persistent identity: annotation1932110
display id: annotation1932110
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932110/range1932110/1
component: BBa_B0055

https://synbiohub.org/public/igem/pSB3K5/annotation1932110/range1932110/1
type: Range
persistent identity: range1932110
display id: range1932110
version: 1
direction: direction/reverse
top level: pSB3K5
start: 2812
end: 2889
orientation: inline

BBa_G0003
type: SequenceAnnotation
persistent identity: annotation1932097
display id: annotation1932097
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932097/range1932097/1
component: BBa_G0003

https://synbiohub.org/public/igem/pSB3K5/annotation1932097/range1932097/1
type: Range
persistent identity: range1932097
display id: range1932097
version: 1
direction: direction/reverse
top level: pSB3K5
start: 177
end: 183
orientation: inline

BBa_P1003
type: SequenceAnnotation
persistent identity: annotation1932103
display id: annotation1932103
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932103/range1932103/1
component: BBa_P1003

https://synbiohub.org/public/igem/pSB3K5/annotation1932103/range1932103/1
type: Range
persistent identity: range1932103
display id: range1932103
version: 1
direction: direction/reverse
top level: pSB3K5
start: 1746
end: 2712
orientation: inline

BBa_G00001
type: SequenceAnnotation
persistent identity: annotation1932078
display id: annotation1932078
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932078/range1932078/1
component: BBa_G00001

https://synbiohub.org/public/igem/pSB3K5/annotation1932078/range1932078/1
type: Range
persistent identity: range1932078
display id: range1932078
version: 1
direction: direction/reverse
top level: pSB3K5
start: 1
end: 21
orientation: inline

BBa_G0002
type: SequenceAnnotation
persistent identity: annotation1932101
display id: annotation1932101
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932101/range1932101/1
component: BBa_G0002

https://synbiohub.org/public/igem/pSB3K5/annotation1932101/range1932101/1
type: Range
persistent identity: range1932101
display id: range1932101
version: 1
direction: direction/reverse
top level: pSB3K5
start: 1738
end: 1745
orientation: inline

BBa_B0053
type: SequenceAnnotation
persistent identity: annotation1932106
display id: annotation1932106
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932106/range1932106/1
component: BBa_B0053

https://synbiohub.org/public/igem/pSB3K5/annotation1932106/range1932106/1
type: Range
persistent identity: range1932106
display id: range1932106
version: 1
direction: direction/reverse
top level: pSB3K5
start: 2720
end: 2791
orientation: inline

BBa_G0004
type: SequenceAnnotation
persistent identity: annotation1932104
display id: annotation1932104
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932104/range1932104/1
component: BBa_G0004

https://synbiohub.org/public/igem/pSB3K5/annotation1932104/range1932104/1
type: Range
persistent identity: range1932104
display id: range1932104
version: 1
direction: direction/reverse
top level: pSB3K5
start: 2713
end: 2719
orientation: inline

BBa_I50032
type: SequenceAnnotation
persistent identity: annotation1932100
display id: annotation1932100
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932100/range1932100/1
component: BBa_I50032

https://synbiohub.org/public/igem/pSB3K5/annotation1932100/range1932100/1
type: Range
persistent identity: range1932100
display id: range1932100
version: 1
direction: direction/reverse
top level: pSB3K5
start: 184
end: 1737
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation1932117
display id: annotation1932117
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932117/range1932117/1
component: BBa_B0042

https://synbiohub.org/public/igem/pSB3K5/annotation1932117/range1932117/1
type: Range
persistent identity: range1932117
display id: range1932117
version: 1
direction: direction/reverse
top level: pSB3K5
start: 2890
end: 2901
orientation: inline

BBa_K864011
type: ComponentDefinition
persistent identity: BBa K864011
display id: BBa_K864011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector with LacI
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB4A15Iq is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and ampicillin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22. This vector carries a LacIq cassette on the backbone for tight repression of any lac promoter cloned into the cloning site.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone.
mutable provenance: Based on BBa_K864003
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K864011_sequence/1

pSB3C5
type: ComponentDefinition
persistent identity: pSB3C5
display id: pSB3C5
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low to medium copy BioBrick standard vector
creator: Reshma Shetty
created: 2007-04-10T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: pSB3C5 is a low to medium copy plasmid based with chloramphenicol resistance. It has a p15A-derived replication origin. pSB3C5 is derived from the BioBricks vector scaffold BBa_I51001.
mutable notes: None.
mutable provenance: BBa_I51001 BioBricks vector scaffold, p15A based origin and a version of the chloramphenicol resistance cassette that has been cleaned of restriction sites.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
component: BBa_G00102
component: BBa_G0002
component: BBa_G0003
component: BBa_G0004
component: BBa_G00001
component: BBa_B0044
component: BBa_B0062
component: BBa_G00100
component: BBa_B0054
component: BBa_G00000
component: BBa_B0042
component: BBa_P1004
component: BBa_B0055
component: BBa_B0043
component: BBa_B0053
component: BBa_I50032
sequence annotation: BBa_B0054
sequence annotation: BBa_G0004
sequence annotation: BBa_G00102
sequence annotation: BBa_B0062
sequence annotation: BBa_B0055
sequence annotation: BBa_B0044
sequence annotation: BBa_G00000
sequence annotation: BBa_G00001
sequence annotation: BBa_G0003
sequence annotation: BBa_B0053
sequence annotation: BBa_B0043
sequence annotation: BBa_G00100
sequence annotation: BBa_B0042
sequence annotation: BBa_I50032
sequence annotation: BBa_P1004
sequence annotation: BBa_G0002
sequence: https://synbiohub.org/public/igem/pSB3C5_sequence/1

BBa_G00102
type: Component
persistent identity: component1928326
display id: component1928326
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00102/1

BBa_G0002
type: Component
persistent identity: component1928333
display id: component1928333
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0002/1

BBa_G0003
type: Component
persistent identity: component1928329
display id: component1928329
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0003/1

BBa_G0004
type: Component
persistent identity: component1928336
display id: component1928336
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0004/1

BBa_G00001
type: Component
persistent identity: component1928310
display id: component1928310
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00001/1

BBa_B0044
type: Component
persistent identity: component1928315
display id: component1928315
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0044/1

BBa_B0062
type: Component
persistent identity: component1928328
display id: component1928328
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0062/1

BBa_G00100
type: Component
persistent identity: component1928339
display id: component1928339
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00100/1

BBa_B0054
type: Component
persistent identity: component1928325
display id: component1928325
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0054/1

BBa_G00000
type: Component
persistent identity: component1928356
display id: component1928356
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00000/1

BBa_B0042
type: Component
persistent identity: component1928349
display id: component1928349
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_P1004
type: Component
persistent identity: component1928335
display id: component1928335
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_P1004/1

BBa_B0055
type: Component
persistent identity: component1928342
display id: component1928342
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0055/1

BBa_B0042
type: Component
persistent identity: component1928322
display id: component1928322
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_B0043
type: Component
persistent identity: component1928354
display id: component1928354
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0043/1

BBa_B0053
type: Component
persistent identity: component1928338
display id: component1928338
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0053/1

BBa_I50032
type: Component
persistent identity: component1928332
display id: component1928332
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_I50032/1

BBa_B0054
type: SequenceAnnotation
persistent identity: annotation1928325
display id: annotation1928325
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928325/range1928325/1
component: BBa_B0054

https://synbiohub.org/public/igem/pSB3C5/annotation1928325/range1928325/1
type: Range
persistent identity: range1928325
display id: range1928325
version: 1
direction: direction/reverse
top level: pSB3C5
start: 47
end: 115
orientation: inline

BBa_G0004
type: SequenceAnnotation
persistent identity: annotation1928336
display id: annotation1928336
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928336/range1928336/1
component: BBa_G0004

https://synbiohub.org/public/igem/pSB3C5/annotation1928336/range1928336/1
type: Range
persistent identity: range1928336
display id: range1928336
version: 1
direction: direction/reverse
top level: pSB3C5
start: 2515
end: 2521
orientation: inline

BBa_G00102
type: SequenceAnnotation
persistent identity: annotation1928326
display id: annotation1928326
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928326/range1928326/1
component: BBa_G00102

https://synbiohub.org/public/igem/pSB3C5/annotation1928326/range1928326/1
type: Range
persistent identity: range1928326
display id: range1928326
version: 1
direction: direction/reverse
top level: pSB3C5
start: 116
end: 135
orientation: inline

BBa_B0062
type: SequenceAnnotation
persistent identity: annotation1928328
display id: annotation1928328
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928328/range1928328/1
component: BBa_B0062

https://synbiohub.org/public/igem/pSB3C5/annotation1928328/range1928328/1
type: Range
persistent identity: range1928328
display id: range1928328
version: 1
direction: direction/reverse
top level: pSB3C5
start: 136
end: 176
orientation: inline

BBa_B0055
type: SequenceAnnotation
persistent identity: annotation1928342
display id: annotation1928342
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928342/range1928342/1
component: BBa_B0055

https://synbiohub.org/public/igem/pSB3C5/annotation1928342/range1928342/1
type: Range
persistent identity: range1928342
display id: range1928342
version: 1
direction: direction/reverse
top level: pSB3C5
start: 2614
end: 2691
orientation: inline

BBa_B0044
type: SequenceAnnotation
persistent identity: annotation1928315
display id: annotation1928315
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928315/range1928315/1
component: BBa_B0044

https://synbiohub.org/public/igem/pSB3C5/annotation1928315/range1928315/1
type: Range
persistent identity: range1928315
display id: range1928315
version: 1
direction: direction/reverse
top level: pSB3C5
start: 22
end: 34
orientation: inline

BBa_G00000
type: SequenceAnnotation
persistent identity: annotation1928356
display id: annotation1928356
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928356/range1928356/1
component: BBa_G00000

https://synbiohub.org/public/igem/pSB3C5/annotation1928356/range1928356/1
type: Range
persistent identity: range1928356
display id: range1928356
version: 1
direction: direction/reverse
top level: pSB3C5
start: 2717
end: 2738
orientation: inline

BBa_G00001
type: SequenceAnnotation
persistent identity: annotation1928310
display id: annotation1928310
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928310/range1928310/1
component: BBa_G00001

https://synbiohub.org/public/igem/pSB3C5/annotation1928310/range1928310/1
type: Range
persistent identity: range1928310
display id: range1928310
version: 1
direction: direction/reverse
top level: pSB3C5
start: 1
end: 21
orientation: inline

BBa_G0003
type: SequenceAnnotation
persistent identity: annotation1928329
display id: annotation1928329
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928329/range1928329/1
component: BBa_G0003

https://synbiohub.org/public/igem/pSB3C5/annotation1928329/range1928329/1
type: Range
persistent identity: range1928329
display id: range1928329
version: 1
direction: direction/reverse
top level: pSB3C5
start: 177
end: 183
orientation: inline

BBa_B0053
type: SequenceAnnotation
persistent identity: annotation1928338
display id: annotation1928338
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928338/range1928338/1
component: BBa_B0053

https://synbiohub.org/public/igem/pSB3C5/annotation1928338/range1928338/1
type: Range
persistent identity: range1928338
display id: range1928338
version: 1
direction: direction/reverse
top level: pSB3C5
start: 2522
end: 2593
orientation: inline

BBa_B0043
type: SequenceAnnotation
persistent identity: annotation1928354
display id: annotation1928354
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928354/range1928354/1
component: BBa_B0043

https://synbiohub.org/public/igem/pSB3C5/annotation1928354/range1928354/1
type: Range
persistent identity: range1928354
display id: range1928354
version: 1
direction: direction/reverse
top level: pSB3C5
start: 2704
end: 2716
orientation: inline

BBa_G00100
type: SequenceAnnotation
persistent identity: annotation1928339
display id: annotation1928339
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928339/range1928339/1
component: BBa_G00100

https://synbiohub.org/public/igem/pSB3C5/annotation1928339/range1928339/1
type: Range
persistent identity: range1928339
display id: range1928339
version: 1
direction: direction/reverse
top level: pSB3C5
start: 2594
end: 2613
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation1928349
display id: annotation1928349
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928349/range1928349/1
component: BBa_B0042

https://synbiohub.org/public/igem/pSB3C5/annotation1928349/range1928349/1
type: Range
persistent identity: range1928349
display id: range1928349
version: 1
direction: direction/reverse
top level: pSB3C5
start: 2692
end: 2703
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation1928322
display id: annotation1928322
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928322/range1928322/1
component: BBa_B0042

https://synbiohub.org/public/igem/pSB3C5/annotation1928322/range1928322/1
type: Range
persistent identity: range1928322
display id: range1928322
version: 1
direction: direction/reverse
top level: pSB3C5
start: 35
end: 46
orientation: inline

BBa_I50032
type: SequenceAnnotation
persistent identity: annotation1928332
display id: annotation1928332
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928332/range1928332/1
component: BBa_I50032

https://synbiohub.org/public/igem/pSB3C5/annotation1928332/range1928332/1
type: Range
persistent identity: range1928332
display id: range1928332
version: 1
direction: direction/reverse
top level: pSB3C5
start: 184
end: 1737
orientation: inline

BBa_P1004
type: SequenceAnnotation
persistent identity: annotation1928335
display id: annotation1928335
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928335/range1928335/1
component: BBa_P1004

https://synbiohub.org/public/igem/pSB3C5/annotation1928335/range1928335/1
type: Range
persistent identity: range1928335
display id: range1928335
version: 1
direction: direction/reverse
top level: pSB3C5
start: 1746
end: 2514
orientation: inline

BBa_G0002
type: SequenceAnnotation
persistent identity: annotation1928333
display id: annotation1928333
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928333/range1928333/1
component: BBa_G0002

https://synbiohub.org/public/igem/pSB3C5/annotation1928333/range1928333/1
type: Range
persistent identity: range1928333
display id: range1928333
version: 1
direction: direction/reverse
top level: pSB3C5
start: 1738
end: 1745
orientation: inline

BB base ve
type: ComponentDefinition
persistent identity: BBa I51020
display id: BBa_I51020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BioBrick base vector (for constructing pSB**5 vectors)
creator: Reshma Shetty
created: 2008-01-05T12:00:00Z
modified: 2015-08-31T04:07:42Z
discontinued: true
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: This is a vector scaffold for constructing new BioBricks vectors. Information on the design is available at http://openwetware.org/wiki/Synthetic_Biology:Vectors/pSB%2A%2A5_design
mutable notes: Documented at http://openwetware.org/wiki/Synthetic_Biology:Vectors/pSB%2A%2A5_design Due to technical difficulties during synthesis, rather than including BBa_P1011 as designed, BBa_P1016 was synthesized as a component of this part. Hence, BBa_I51000 documents the original design and BBa_I51001 is the vector scaffold as synthesized. BBa_I52002 is the base vector as corrected from the synthesized construct.
mutable provenance: This is a composite part generated from several basic parts obtained from a variety of sources.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
component: BBa_B0045
component: BBa_B0044
component: BBa_G00102
component: BBa_I50022
component: BBa_B0053
component: BBa_B0043
component: BBa_B0042
component: BBa_B0055
component: BBa_G00001
component: BBa_G00100
component: BBa_B0062
component: BBa_P1006
component: BBa_B0054
component: BBa_P1016
component: BBa_G00000
sequence annotation: BBa_B0054
sequence annotation: BBa_B0062
sequence annotation: BBa_B0043
sequence annotation: BBa_B0045
sequence annotation: BBa_B0053
sequence annotation: BBa_B0055
sequence annotation: BBa_B0042
sequence annotation: BBa_P1006
sequence annotation: BBa_P1016
sequence annotation: BBa_G00102
sequence annotation: BBa_I50022
sequence annotation: BBa_G00000
sequence annotation: BBa_G00100
sequence annotation: BBa_B0044
sequence annotation: BBa_G00001
sequence: https://synbiohub.org/public/igem/BBa_I51020_sequence/1

BBa_B0045
type: Component
persistent identity: component1959240
display id: component1959240
version: 1
top level: BB base ve
access: public
definition: https://synbiohub.org/public/igem/BBa_B0045/1

BBa_B0044
type: Component
persistent identity: component1959218
display id: component1959218
version: 1
top level: BB base ve
access: public
definition: https://synbiohub.org/public/igem/BBa_B0044/1

BBa_G00102
type: Component
persistent identity: component1959229
display id: component1959229
version: 1
top level: BB base ve
access: public
definition: https://synbiohub.org/public/igem/BBa_G00102/1

BBa_I50022
type: Component
persistent identity: component1959277
display id: component1959277
version: 1
top level: BB base ve
access: public
definition: https://synbiohub.org/public/igem/BBa_I50022/1

BBa_B0053
type: Component
persistent identity: component1959242
display id: component1959242
version: 1
top level: BB base ve
access: public
definition: https://synbiohub.org/public/igem/BBa_B0053/1

BBa_B0043
type: Component
persistent identity: component1959259
display id: component1959259
version: 1
top level: BB base ve
access: public
definition: https://synbiohub.org/public/igem/BBa_B0043/1

BBa_B0042
type: Component
persistent identity: component1959254
display id: component1959254
version: 1
top level: BB base ve
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_B0055
type: Component
persistent identity: component1959247
display id: component1959247
version: 1
top level: BB base ve
access: public
definition: https://synbiohub.org/public/igem/BBa_B0055/1

BBa_G00001
type: Component
persistent identity: component1959213
display id: component1959213
version: 1
top level: BB base ve
access: public
definition: https://synbiohub.org/public/igem/BBa_G00001/1

BBa_G00100
type: Component
persistent identity: component1959244
display id: component1959244
version: 1
top level: BB base ve
access: public
definition: https://synbiohub.org/public/igem/BBa_G00100/1

BBa_B0062
type: Component
persistent identity: component1959231
display id: component1959231
version: 1
top level: BB base ve
access: public
definition: https://synbiohub.org/public/igem/BBa_B0062/1

BBa_B0042
type: Component
persistent identity: component1959225
display id: component1959225
version: 1
top level: BB base ve
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_P1006
type: Component
persistent identity: component1959238
display id: component1959238
version: 1
top level: BB base ve
access: public
definition: https://synbiohub.org/public/igem/BBa_P1006/1

BBa_B0045
type: Component
persistent identity: component1959233
display id: component1959233
version: 1
top level: BB base ve
access: public
definition: https://synbiohub.org/public/igem/BBa_B0045/1

BBa_B0054
type: Component
persistent identity: component1959228
display id: component1959228
version: 1
top level: BB base ve
access: public
definition: https://synbiohub.org/public/igem/BBa_B0054/1

BBa_P1016
type: Component
persistent identity: component1959267
display id: component1959267
version: 1
top level: BB base ve
access: public
definition: https://synbiohub.org/public/igem/BBa_P1016/1

BBa_G00000
type: Component
persistent identity: component1959263
display id: component1959263
version: 1
top level: BB base ve
access: public
definition: https://synbiohub.org/public/igem/BBa_G00000/1

BBa_B0054
type: SequenceAnnotation
persistent identity: annotation1959228
display id: annotation1959228
version: 1
top level: BB base ve
location: https://synbiohub.org/public/igem/BBa_I51020/annotation1959228/range1959228/1
component: BBa_B0054

https://synbiohub.org/public/igem/BBa_I51020/annotation1959228/range1959228/1
type: Range
persistent identity: range1959228
display id: range1959228
version: 1
direction: direction/reverse
top level: BB base ve
start: 47
end: 115
orientation: inline

BBa_B0062
type: SequenceAnnotation
persistent identity: annotation1959231
display id: annotation1959231
version: 1
top level: BB base ve
location: https://synbiohub.org/public/igem/BBa_I51020/annotation1959231/range1959231/1
component: BBa_B0062

https://synbiohub.org/public/igem/BBa_I51020/annotation1959231/range1959231/1
type: Range
persistent identity: range1959231
display id: range1959231
version: 1
direction: direction/reverse
top level: BB base ve
start: 136
end: 176
orientation: inline

BBa_B0043
type: SequenceAnnotation
persistent identity: annotation1959259
display id: annotation1959259
version: 1
top level: BB base ve
location: https://synbiohub.org/public/igem/BBa_I51020/annotation1959259/range1959259/1
component: BBa_B0043

https://synbiohub.org/public/igem/BBa_I51020/annotation1959259/range1959259/1
type: Range
persistent identity: range1959259
display id: range1959259
version: 1
direction: direction/reverse
top level: BB base ve
start: 1314
end: 1326
orientation: inline

BBa_B0045
type: SequenceAnnotation
persistent identity: annotation1959240
display id: annotation1959240
version: 1
top level: BB base ve
location: https://synbiohub.org/public/igem/BBa_I51020/annotation1959240/range1959240/1
component: BBa_B0045

https://synbiohub.org/public/igem/BBa_I51020/annotation1959240/range1959240/1
type: Range
persistent identity: range1959240
display id: range1959240
version: 1
direction: direction/reverse
top level: BB base ve
start: 1126
end: 1131
orientation: inline

BBa_B0053
type: SequenceAnnotation
persistent identity: annotation1959242
display id: annotation1959242
version: 1
top level: BB base ve
location: https://synbiohub.org/public/igem/BBa_I51020/annotation1959242/range1959242/1
component: BBa_B0053

https://synbiohub.org/public/igem/BBa_I51020/annotation1959242/range1959242/1
type: Range
persistent identity: range1959242
display id: range1959242
version: 1
direction: direction/reverse
top level: BB base ve
start: 1132
end: 1203
orientation: inline

BBa_B0055
type: SequenceAnnotation
persistent identity: annotation1959247
display id: annotation1959247
version: 1
top level: BB base ve
location: https://synbiohub.org/public/igem/BBa_I51020/annotation1959247/range1959247/1
component: BBa_B0055

https://synbiohub.org/public/igem/BBa_I51020/annotation1959247/range1959247/1
type: Range
persistent identity: range1959247
display id: range1959247
version: 1
direction: direction/reverse
top level: BB base ve
start: 1224
end: 1301
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation1959254
display id: annotation1959254
version: 1
top level: BB base ve
location: https://synbiohub.org/public/igem/BBa_I51020/annotation1959254/range1959254/1
component: BBa_B0042

https://synbiohub.org/public/igem/BBa_I51020/annotation1959254/range1959254/1
type: Range
persistent identity: range1959254
display id: range1959254
version: 1
direction: direction/reverse
top level: BB base ve
start: 1302
end: 1313
orientation: inline

BBa_P1006
type: SequenceAnnotation
persistent identity: annotation1959238
display id: annotation1959238
version: 1
top level: BB base ve
location: https://synbiohub.org/public/igem/BBa_I51020/annotation1959238/range1959238/1
component: BBa_P1006

https://synbiohub.org/public/igem/BBa_I51020/annotation1959238/range1959238/1
type: Range
persistent identity: range1959238
display id: range1959238
version: 1
direction: direction/reverse
top level: BB base ve
start: 183
end: 1125
orientation: inline

BBa_P1016
type: SequenceAnnotation
persistent identity: annotation1959267
display id: annotation1959267
version: 1
top level: BB base ve
location: https://synbiohub.org/public/igem/BBa_I51020/annotation1959267/range1959267/1
component: BBa_P1016

https://synbiohub.org/public/igem/BBa_I51020/annotation1959267/range1959267/1
type: Range
persistent identity: range1959267
display id: range1959267
version: 1
direction: direction/reverse
top level: BB base ve
start: 1349
end: 1764
orientation: inline

BBa_G00102
type: SequenceAnnotation
persistent identity: annotation1959229
display id: annotation1959229
version: 1
top level: BB base ve
location: https://synbiohub.org/public/igem/BBa_I51020/annotation1959229/range1959229/1
component: BBa_G00102

https://synbiohub.org/public/igem/BBa_I51020/annotation1959229/range1959229/1
type: Range
persistent identity: range1959229
display id: range1959229
version: 1
direction: direction/reverse
top level: BB base ve
start: 116
end: 135
orientation: inline

BBa_I50022
type: SequenceAnnotation
persistent identity: annotation1959277
display id: annotation1959277
version: 1
top level: BB base ve
location: https://synbiohub.org/public/igem/BBa_I51020/annotation1959277/range1959277/1
component: BBa_I50022

https://synbiohub.org/public/igem/BBa_I51020/annotation1959277/range1959277/1
type: Range
persistent identity: range1959277
display id: range1959277
version: 1
direction: direction/reverse
top level: BB base ve
start: 1765
end: 2438
orientation: inline

BBa_B0045
type: SequenceAnnotation
persistent identity: annotation1959233
display id: annotation1959233
version: 1
top level: BB base ve
location: https://synbiohub.org/public/igem/BBa_I51020/annotation1959233/range1959233/1
component: BBa_B0045

https://synbiohub.org/public/igem/BBa_I51020/annotation1959233/range1959233/1
type: Range
persistent identity: range1959233
display id: range1959233
version: 1
direction: direction/reverse
top level: BB base ve
start: 177
end: 182
orientation: inline

BBa_G00000
type: SequenceAnnotation
persistent identity: annotation1959263
display id: annotation1959263
version: 1
top level: BB base ve
location: https://synbiohub.org/public/igem/BBa_I51020/annotation1959263/range1959263/1
component: BBa_G00000

https://synbiohub.org/public/igem/BBa_I51020/annotation1959263/range1959263/1
type: Range
persistent identity: range1959263
display id: range1959263
version: 1
direction: direction/reverse
top level: BB base ve
start: 1327
end: 1348
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation1959225
display id: annotation1959225
version: 1
top level: BB base ve
location: https://synbiohub.org/public/igem/BBa_I51020/annotation1959225/range1959225/1
component: BBa_B0042

https://synbiohub.org/public/igem/BBa_I51020/annotation1959225/range1959225/1
type: Range
persistent identity: range1959225
display id: range1959225
version: 1
direction: direction/reverse
top level: BB base ve
start: 35
end: 46
orientation: inline

BBa_G00100
type: SequenceAnnotation
persistent identity: annotation1959244
display id: annotation1959244
version: 1
top level: BB base ve
location: https://synbiohub.org/public/igem/BBa_I51020/annotation1959244/range1959244/1
component: BBa_G00100

https://synbiohub.org/public/igem/BBa_I51020/annotation1959244/range1959244/1
type: Range
persistent identity: range1959244
display id: range1959244
version: 1
direction: direction/reverse
top level: BB base ve
start: 1204
end: 1223
orientation: inline

BBa_B0044
type: SequenceAnnotation
persistent identity: annotation1959218
display id: annotation1959218
version: 1
top level: BB base ve
location: https://synbiohub.org/public/igem/BBa_I51020/annotation1959218/range1959218/1
component: BBa_B0044

https://synbiohub.org/public/igem/BBa_I51020/annotation1959218/range1959218/1
type: Range
persistent identity: range1959218
display id: range1959218
version: 1
direction: direction/reverse
top level: BB base ve
start: 22
end: 34
orientation: inline

BBa_G00001
type: SequenceAnnotation
persistent identity: annotation1959213
display id: annotation1959213
version: 1
top level: BB base ve
location: https://synbiohub.org/public/igem/BBa_I51020/annotation1959213/range1959213/1
component: BBa_G00001

https://synbiohub.org/public/igem/BBa_I51020/annotation1959213/range1959213/1
type: Range
persistent identity: range1959213
display id: range1959213
version: 1
direction: direction/reverse
top level: BB base ve
start: 1
end: 21
orientation: inline

BBa_K864007
type: ComponentDefinition
persistent identity: BBa K864007
display id: BBa_K864007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB4S15(FRT) is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and spectinomycin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone. The pSC101 origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
mutable provenance: Based on pSB3T5
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K864007_sequence/1

pSB8S15
type: ComponentDefinition
persistent identity: BBa K864019
display id: BBa_K864019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick temperature sensitive standard vector
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB8S15 is a BioBrick standard vector with low copy pSC101ts replication origin (~5 copies per cell) and spectinomycin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The pSC101ts is a temperature sensitive pSC101 repelication origin and propagates at 30???C but stop replicating at 42???C. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone. The pSC101ts origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
mutable provenance: Based on BBa_K864017
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K864019_sequence/1

BBa_K864000
type: ComponentDefinition
persistent identity: BBa K864000
display id: BBa_K864000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector
creator: Erik Lundin
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB4A15 is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and ??? antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone. The pSC101 origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
mutable provenance: Based on pSB3T5
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: T3 terminator
sequence annotation: Lambda Red reverse primer
sequence annotation: -10
sequence annotation: rrnC terminator
sequence annotation: Tandem repeat
sequence annotation: repA
sequence annotation: pSC101 ori
sequence annotation: Repeat "4"
sequence annotation: VR
sequence annotation: Mutated A>T to remove SpeI
sequence annotation: Repeat "5"
sequence annotation: Lux terminator
sequence annotation: RBS
sequence annotation: -35
sequence annotation: tonB terminator
sequence annotation: VF2
sequence annotation: Lambda Red forward primer
sequence annotation: ampR BBa_P1002
sequence: https://synbiohub.org/public/igem/BBa_K864000_sequence/1

T3 terminator
type: SequenceAnnotation
persistent identity: annotation2198076
display id: annotation2198076
version: 1
top level: BBa_K864000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198076/range2198076/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198076/range2198076/1
type: Range
persistent identity: range2198076
display id: range2198076
version: 1
direction: direction/forward
top level: BBa_K864000
start: 3515
end: 3592
orientation: inline

Lambda Red reverse primer
type: SequenceAnnotation
persistent identity: annotation2198031
display id: annotation2198031
version: 1
top level: BBa_K864000
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198031/range2198031/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198031/range2198031/1
type: Range
persistent identity: range2198031
display id: range2198031
version: 1
direction: direction/reverse
top level: BBa_K864000
start: 18
end: 38
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2198006
display id: annotation2198006
version: 1
top level: BBa_K864000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198006/range2198006/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198006/range2198006/1
type: Range
persistent identity: range2198006
display id: range2198006
version: 1
direction: direction/forward
top level: BBa_K864000
start: 903
end: 908
orientation: inline

rrnC terminator
type: SequenceAnnotation
persistent identity: annotation2198093
display id: annotation2198093
version: 1
top level: BBa_K864000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198093/range2198093/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198093/range2198093/1
type: Range
persistent identity: range2198093
display id: range2198093
version: 1
direction: direction/forward
top level: BBa_K864000
start: 136
end: 175
orientation: inline

Tandem repeat
type: SequenceAnnotation
persistent identity: annotation2198039
display id: annotation2198039
version: 1
top level: BBa_K864000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198039/range2198039/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198039/range2198039/1
type: Range
persistent identity: range2198039
display id: range2198039
version: 1
direction: direction/forward
top level: BBa_K864000
start: 820
end: 841
orientation: inline

repA
type: SequenceAnnotation
persistent identity: annotation2198011
display id: annotation2198011
version: 1
top level: BBa_K864000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198011/range2198011/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198011/range2198011/1
type: Range
persistent identity: range2198011
display id: range2198011
version: 1
direction: direction/forward
top level: BBa_K864000
start: 939
end: 1889
orientation: inline

Tandem repeat
type: SequenceAnnotation
persistent identity: annotation2198036
display id: annotation2198036
version: 1
top level: BBa_K864000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198036/range2198036/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198036/range2198036/1
type: Range
persistent identity: range2198036
display id: range2198036
version: 1
direction: direction/forward
top level: BBa_K864000
start: 790
end: 809
orientation: inline

pSC101 ori
type: SequenceAnnotation
persistent identity: annotation2198057
display id: annotation2198057
version: 1
top level: BBa_K864000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198057/range2198057/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198057/range2198057/1
type: Range
persistent identity: range2198057
display id: range2198057
version: 1
direction: direction/forward
top level: BBa_K864000
start: 190
end: 2405
orientation: inline

Repeat "4"
type: SequenceAnnotation
persistent identity: annotation2198038
display id: annotation2198038
version: 1
top level: BBa_K864000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198038/range2198038/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198038/range2198038/1
type: Range
persistent identity: range2198038
display id: range2198038
version: 1
direction: direction/forward
top level: BBa_K864000
start: 862
end: 880
orientation: inline

Tandem repeat
type: SequenceAnnotation
persistent identity: annotation2198037
display id: annotation2198037
version: 1
top level: BBa_K864000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198037/range2198037/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198037/range2198037/1
type: Range
persistent identity: range2198037
display id: range2198037
version: 1
direction: direction/forward
top level: BBa_K864000
start: 767
end: 788
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation2198033
display id: annotation2198033
version: 1
top level: BBa_K864000
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198033/range2198033/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198033/range2198033/1
type: Range
persistent identity: range2198033
display id: range2198033
version: 1
direction: direction/reverse
top level: BBa_K864000
start: 116
end: 135
orientation: inline

Mutated A>T to remove SpeI
type: SequenceAnnotation
persistent identity: annotation2198029
display id: annotation2198029
version: 1
top level: BBa_K864000
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198029/range2198029/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198029/range2198029/1
type: Range
persistent identity: range2198029
display id: range2198029
version: 1
direction: direction/forward
top level: BBa_K864000
start: 867
end: 867
orientation: inline

Repeat "5"
type: SequenceAnnotation
persistent identity: annotation2198056
display id: annotation2198056
version: 1
top level: BBa_K864000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198056/range2198056/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198056/range2198056/1
type: Range
persistent identity: range2198056
display id: range2198056
version: 1
direction: direction/forward
top level: BBa_K864000
start: 891
end: 912
orientation: inline

Lux terminator
type: SequenceAnnotation
persistent identity: annotation2198075
display id: annotation2198075
version: 1
top level: BBa_K864000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198075/range2198075/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198075/range2198075/1
type: Range
persistent identity: range2198075
display id: range2198075
version: 1
direction: direction/reverse
top level: BBa_K864000
start: 2454
end: 2499
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2198034
display id: annotation2198034
version: 1
top level: BBa_K864000
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198034/range2198034/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198034/range2198034/1
type: Range
persistent identity: range2198034
display id: range2198034
version: 1
direction: direction/forward
top level: BBa_K864000
start: 3374
end: 3378
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2198010
display id: annotation2198010
version: 1
top level: BBa_K864000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198010/range2198010/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198010/range2198010/1
type: Range
persistent identity: range2198010
display id: range2198010
version: 1
direction: direction/reverse
top level: BBa_K864000
start: 3413
end: 3418
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2198007
display id: annotation2198007
version: 1
top level: BBa_K864000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198007/range2198007/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198007/range2198007/1
type: Range
persistent identity: range2198007
display id: range2198007
version: 1
direction: direction/reverse
top level: BBa_K864000
start: 3387
end: 3392
orientation: inline

tonB terminator
type: SequenceAnnotation
persistent identity: annotation2198074
display id: annotation2198074
version: 1
top level: BBa_K864000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198074/range2198074/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198074/range2198074/1
type: Range
persistent identity: range2198074
display id: range2198074
version: 1
direction: direction/forward
top level: BBa_K864000
start: 47
end: 115
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation2198032
display id: annotation2198032
version: 1
top level: BBa_K864000
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198032/range2198032/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198032/range2198032/1
type: Range
persistent identity: range2198032
display id: range2198032
version: 1
direction: direction/forward
top level: BBa_K864000
start: 3495
end: 3514
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2198009
display id: annotation2198009
version: 1
top level: BBa_K864000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198009/range2198009/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198009/range2198009/1
type: Range
persistent identity: range2198009
display id: range2198009
version: 1
direction: direction/forward
top level: BBa_K864000
start: 881
end: 886
orientation: inline

Lambda Red forward primer
type: SequenceAnnotation
persistent identity: annotation2198030
display id: annotation2198030
version: 1
top level: BBa_K864000
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198030/range2198030/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198030/range2198030/1
type: Range
persistent identity: range2198030
display id: range2198030
version: 1
direction: direction/forward
top level: BBa_K864000
start: 2433
end: 2450
orientation: inline

ampR BBa_P1002
type: SequenceAnnotation
persistent identity: annotation2198012
display id: annotation2198012
version: 1
top level: BBa_K864000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198012/range2198012/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198012/range2198012/1
type: Range
persistent identity: range2198012
display id: range2198012
version: 1
direction: direction/reverse
top level: BBa_K864000
start: 2509
end: 3369
orientation: inline

pSB3T5
type: ComponentDefinition
persistent identity: pSB3T5
display id: pSB3T5
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low to medium copy BioBrick standard vector
creator: Reshma Shetty
created: 2007-04-10T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: pSB3T5 is a low to medium copy plasmid based with tetracycline resistance. It has a p15A-derived replication origin. pSB3T5 is derived from the BioBricks vector scaffold BBa_I51001.
mutable notes: None.
mutable provenance: BBa_I51001 BioBricks vector scaffold, p15A based origin and a version of the tetracycline resistance cassette that has been cleaned of restriction sites.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
component: BBa_G0002
component: BBa_G0003
component: BBa_B0055
component: BBa_G00102
component: BBa_B0042
component: BBa_G0004
component: BBa_G00100
component: BBa_B0043
component: BBa_G00001
component: BBa_P1005
component: BBa_I50032
component: BBa_B0054
component: BBa_G00000
component: BBa_B0062
component: BBa_B0044
component: BBa_B0053
sequence annotation: BBa_G00001
sequence annotation: BBa_G00102
sequence annotation: BBa_G0003
sequence annotation: BBa_B0054
sequence annotation: BBa_G00100
sequence annotation: BBa_B0043
sequence annotation: BBa_B0053
sequence annotation: BBa_B0042
sequence annotation: BBa_B0044
sequence annotation: BBa_B0062
sequence annotation: BBa_G0004
sequence annotation: BBa_G00000
sequence annotation: BBa_B0055
sequence annotation: BBa_I50032
sequence annotation: BBa_P1005
sequence annotation: BBa_G0002
sequence: https://synbiohub.org/public/igem/pSB3T5_sequence/1

BBa_G0002
type: Component
persistent identity: component1928381
display id: component1928381
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0002/1

BBa_G0003
type: Component
persistent identity: component1928377
display id: component1928377
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0003/1

BBa_B0055
type: Component
persistent identity: component1928391
display id: component1928391
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0055/1

BBa_G00102
type: Component
persistent identity: component1928374
display id: component1928374
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00102/1

BBa_B0042
type: Component
persistent identity: component1928398
display id: component1928398
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_G0004
type: Component
persistent identity: component1928385
display id: component1928385
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0004/1

BBa_G00100
type: Component
persistent identity: component1928388
display id: component1928388
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00100/1

BBa_B0043
type: Component
persistent identity: component1928403
display id: component1928403
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0043/1

BBa_B0042
type: Component
persistent identity: component1928370
display id: component1928370
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_G00001
type: Component
persistent identity: component1928358
display id: component1928358
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00001/1

BBa_P1005
type: Component
persistent identity: component1928384
display id: component1928384
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_P1005/1

BBa_I50032
type: Component
persistent identity: component1928380
display id: component1928380
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_I50032/1

BBa_B0054
type: Component
persistent identity: component1928373
display id: component1928373
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0054/1

BBa_G00000
type: Component
persistent identity: component1928405
display id: component1928405
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00000/1

BBa_B0062
type: Component
persistent identity: component1928376
display id: component1928376
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0062/1

BBa_B0044
type: Component
persistent identity: component1928363
display id: component1928363
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0044/1

BBa_B0053
type: Component
persistent identity: component1928387
display id: component1928387
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0053/1

BBa_G00001
type: SequenceAnnotation
persistent identity: annotation1928358
display id: annotation1928358
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928358/range1928358/1
component: BBa_G00001

https://synbiohub.org/public/igem/pSB3T5/annotation1928358/range1928358/1
type: Range
persistent identity: range1928358
display id: range1928358
version: 1
direction: direction/reverse
top level: pSB3T5
start: 1
end: 21
orientation: inline

BBa_G00102
type: SequenceAnnotation
persistent identity: annotation1928374
display id: annotation1928374
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928374/range1928374/1
component: BBa_G00102

https://synbiohub.org/public/igem/pSB3T5/annotation1928374/range1928374/1
type: Range
persistent identity: range1928374
display id: range1928374
version: 1
direction: direction/reverse
top level: pSB3T5
start: 116
end: 135
orientation: inline

BBa_G0003
type: SequenceAnnotation
persistent identity: annotation1928377
display id: annotation1928377
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928377/range1928377/1
component: BBa_G0003

https://synbiohub.org/public/igem/pSB3T5/annotation1928377/range1928377/1
type: Range
persistent identity: range1928377
display id: range1928377
version: 1
direction: direction/reverse
top level: pSB3T5
start: 177
end: 183
orientation: inline

BBa_B0054
type: SequenceAnnotation
persistent identity: annotation1928373
display id: annotation1928373
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928373/range1928373/1
component: BBa_B0054

https://synbiohub.org/public/igem/pSB3T5/annotation1928373/range1928373/1
type: Range
persistent identity: range1928373
display id: range1928373
version: 1
direction: direction/reverse
top level: pSB3T5
start: 47
end: 115
orientation: inline

BBa_G00100
type: SequenceAnnotation
persistent identity: annotation1928388
display id: annotation1928388
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928388/range1928388/1
component: BBa_G00100

https://synbiohub.org/public/igem/pSB3T5/annotation1928388/range1928388/1
type: Range
persistent identity: range1928388
display id: range1928388
version: 1
direction: direction/reverse
top level: pSB3T5
start: 3108
end: 3127
orientation: inline

BBa_B0043
type: SequenceAnnotation
persistent identity: annotation1928403
display id: annotation1928403
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928403/range1928403/1
component: BBa_B0043

https://synbiohub.org/public/igem/pSB3T5/annotation1928403/range1928403/1
type: Range
persistent identity: range1928403
display id: range1928403
version: 1
direction: direction/reverse
top level: pSB3T5
start: 3218
end: 3230
orientation: inline

BBa_B0053
type: SequenceAnnotation
persistent identity: annotation1928387
display id: annotation1928387
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928387/range1928387/1
component: BBa_B0053

https://synbiohub.org/public/igem/pSB3T5/annotation1928387/range1928387/1
type: Range
persistent identity: range1928387
display id: range1928387
version: 1
direction: direction/reverse
top level: pSB3T5
start: 3036
end: 3107
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation1928370
display id: annotation1928370
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928370/range1928370/1
component: BBa_B0042

https://synbiohub.org/public/igem/pSB3T5/annotation1928370/range1928370/1
type: Range
persistent identity: range1928370
display id: range1928370
version: 1
direction: direction/reverse
top level: pSB3T5
start: 35
end: 46
orientation: inline

BBa_B0044
type: SequenceAnnotation
persistent identity: annotation1928363
display id: annotation1928363
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928363/range1928363/1
component: BBa_B0044

https://synbiohub.org/public/igem/pSB3T5/annotation1928363/range1928363/1
type: Range
persistent identity: range1928363
display id: range1928363
version: 1
direction: direction/reverse
top level: pSB3T5
start: 22
end: 34
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation1928398
display id: annotation1928398
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928398/range1928398/1
component: BBa_B0042

https://synbiohub.org/public/igem/pSB3T5/annotation1928398/range1928398/1
type: Range
persistent identity: range1928398
display id: range1928398
version: 1
direction: direction/reverse
top level: pSB3T5
start: 3206
end: 3217
orientation: inline

BBa_B0062
type: SequenceAnnotation
persistent identity: annotation1928376
display id: annotation1928376
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928376/range1928376/1
component: BBa_B0062

https://synbiohub.org/public/igem/pSB3T5/annotation1928376/range1928376/1
type: Range
persistent identity: range1928376
display id: range1928376
version: 1
direction: direction/reverse
top level: pSB3T5
start: 136
end: 176
orientation: inline

BBa_G0004
type: SequenceAnnotation
persistent identity: annotation1928385
display id: annotation1928385
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928385/range1928385/1
component: BBa_G0004

https://synbiohub.org/public/igem/pSB3T5/annotation1928385/range1928385/1
type: Range
persistent identity: range1928385
display id: range1928385
version: 1
direction: direction/reverse
top level: pSB3T5
start: 3029
end: 3035
orientation: inline

BBa_G00000
type: SequenceAnnotation
persistent identity: annotation1928405
display id: annotation1928405
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928405/range1928405/1
component: BBa_G00000

https://synbiohub.org/public/igem/pSB3T5/annotation1928405/range1928405/1
type: Range
persistent identity: range1928405
display id: range1928405
version: 1
direction: direction/reverse
top level: pSB3T5
start: 3231
end: 3252
orientation: inline

BBa_B0055
type: SequenceAnnotation
persistent identity: annotation1928391
display id: annotation1928391
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928391/range1928391/1
component: BBa_B0055

https://synbiohub.org/public/igem/pSB3T5/annotation1928391/range1928391/1
type: Range
persistent identity: range1928391
display id: range1928391
version: 1
direction: direction/reverse
top level: pSB3T5
start: 3128
end: 3205
orientation: inline

BBa_I50032
type: SequenceAnnotation
persistent identity: annotation1928380
display id: annotation1928380
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928380/range1928380/1
component: BBa_I50032

https://synbiohub.org/public/igem/pSB3T5/annotation1928380/range1928380/1
type: Range
persistent identity: range1928380
display id: range1928380
version: 1
direction: direction/reverse
top level: pSB3T5
start: 184
end: 1737
orientation: inline

BBa_P1005
type: SequenceAnnotation
persistent identity: annotation1928384
display id: annotation1928384
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928384/range1928384/1
component: BBa_P1005

https://synbiohub.org/public/igem/pSB3T5/annotation1928384/range1928384/1
type: Range
persistent identity: range1928384
display id: range1928384
version: 1
direction: direction/reverse
top level: pSB3T5
start: 1746
end: 3028
orientation: inline

BBa_G0002
type: SequenceAnnotation
persistent identity: annotation1928381
display id: annotation1928381
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928381/range1928381/1
component: BBa_G0002

https://synbiohub.org/public/igem/pSB3T5/annotation1928381/range1928381/1
type: Range
persistent identity: range1928381
display id: range1928381
version: 1
direction: direction/reverse
top level: pSB3T5
start: 1738
end: 1745
orientation: inline

BBa_K864012
type: ComponentDefinition
persistent identity: BBa K864012
display id: BBa_K864012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector with LacI
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB4A15Iq(FRT) is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and ampicillin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22. This vector carries a LacIq cassette on the backbone for tight repression of any lac promoter cloned into the cloning site.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone.
mutable provenance: Based on BBa_K864005
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K864012_sequence/1

BBa_K864010
type: ComponentDefinition
persistent identity: BBa K864010
display id: BBa_K864010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector with LacI
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB4K15Iq is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and kanamycin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22. This vector carries a LacIq cassette on the backbone for tight repression of any lac promoter cloned into the cloning site.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone.
mutable provenance: Based on BBa_K864002
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K864010_sequence/1

BBa_K864005
type: ComponentDefinition
persistent identity: BBa K864005
display id: BBa_K864005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector
creator: Erik Lundin
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB4A15 is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and ampicillin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22. The resistance cassette is flanked by FRT sites (BBa_J61020) which allows for subsequent removal of the resistance cassette from the chromosome by the FLP recombinase. The pSB8x15 series are especially suitable for carrying the FLP recombinase, since they can be removed by incubation at 42??C.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone. The pSC101 origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
mutable provenance: Based on pSB3T5
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: VR
sequence annotation: Lux terminator
sequence annotation: Lambda Red reverse primer
sequence annotation: pSC101 ori BBa_K864050
sequence annotation: rrnC terminator
sequence annotation: Lambda Red forward primer
sequence annotation: TonB terminator
sequence: https://synbiohub.org/public/igem/BBa_K864005_sequence/1

VR
type: SequenceAnnotation
persistent identity: annotation2200046
display id: annotation2200046
version: 1
top level: BBa_K864005
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864005/annotation2200046/range2200046/1

https://synbiohub.org/public/igem/BBa_K864005/annotation2200046/range2200046/1
type: Range
persistent identity: range2200046
display id: range2200046
version: 1
direction: direction/reverse
top level: BBa_K864005
start: 116
end: 135
orientation: inline

Lux terminator
type: SequenceAnnotation
persistent identity: annotation2200051
display id: annotation2200051
version: 1
top level: BBa_K864005
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864005/annotation2200051/range2200051/1

https://synbiohub.org/public/igem/BBa_K864005/annotation2200051/range2200051/1
type: Range
persistent identity: range2200051
display id: range2200051
version: 1
direction: direction/forward
top level: BBa_K864005
start: 2454
end: 2499
orientation: inline

Lambda Red reverse primer
type: SequenceAnnotation
persistent identity: annotation2200041
display id: annotation2200041
version: 1
top level: BBa_K864005
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864005/annotation2200041/range2200041/1

https://synbiohub.org/public/igem/BBa_K864005/annotation2200041/range2200041/1
type: Range
persistent identity: range2200041
display id: range2200041
version: 1
direction: direction/reverse
top level: BBa_K864005
start: 18
end: 38
orientation: inline

pSC101 ori BBa_K864050
type: SequenceAnnotation
persistent identity: annotation2200048
display id: annotation2200048
version: 1
top level: BBa_K864005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864005/annotation2200048/range2200048/1

https://synbiohub.org/public/igem/BBa_K864005/annotation2200048/range2200048/1
type: Range
persistent identity: range2200048
display id: range2200048
version: 1
direction: direction/forward
top level: BBa_K864005
start: 190
end: 2405
orientation: inline

rrnC terminator
type: SequenceAnnotation
persistent identity: annotation2200047
display id: annotation2200047
version: 1
top level: BBa_K864005
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864005/annotation2200047/range2200047/1

https://synbiohub.org/public/igem/BBa_K864005/annotation2200047/range2200047/1
type: Range
persistent identity: range2200047
display id: range2200047
version: 1
direction: direction/forward
top level: BBa_K864005
start: 136
end: 175
orientation: inline

Lambda Red forward primer
type: SequenceAnnotation
persistent identity: annotation2200050
display id: annotation2200050
version: 1
top level: BBa_K864005
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864005/annotation2200050/range2200050/1

https://synbiohub.org/public/igem/BBa_K864005/annotation2200050/range2200050/1
type: Range
persistent identity: range2200050
display id: range2200050
version: 1
direction: direction/forward
top level: BBa_K864005
start: 2433
end: 2450
orientation: inline

TonB terminator
type: SequenceAnnotation
persistent identity: annotation2200045
display id: annotation2200045
version: 1
top level: BBa_K864005
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864005/annotation2200045/range2200045/1

https://synbiohub.org/public/igem/BBa_K864005/annotation2200045/range2200045/1
type: Range
persistent identity: range2200045
display id: range2200045
version: 1
direction: direction/forward
top level: BBa_K864005
start: 47
end: 115
orientation: inline

pSB4T5
type: ComponentDefinition
persistent identity: pSB4T5
display id: pSB4T5
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector
creator: Reshma Shetty
created: 2007-02-28T12:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: pSB4T3 is a low copy plasmid with tetracycline resistance. It has a repA pSC101-derived replication origin. (Copy number ~10-12, Lutz and Bujard, 1997.) It is constructed from the BioBricks vector scaffold BBa_I51001.
mutable notes: See http://openwetware.org/wiki/Synthetic_Biology:Vectors/pSB%2A%2A5_design for more information.
mutable provenance: BBa_I51001 vector scaffold, BBa_I50040 replication origin and BBa_P1005 resistance marker.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
component: BBa_B0053
component: BBa_G0003
component: BBa_B0055
component: BBa_B0042
component: BBa_P1005
component: BBa_B0043
component: BBa_G00100
component: BBa_G00001
component: BBa_B0062
component: BBa_B0044
component: BBa_G00102
component: BBa_B0054
component: BBa_G0002
component: BBa_I50042
component: BBa_G0004
component: BBa_G00000
sequence annotation: BBa_G0003
sequence annotation: BBa_G0004
sequence annotation: BBa_B0053
sequence annotation: BBa_B0042
sequence annotation: BBa_P1005
sequence annotation: BBa_B0054
sequence annotation: BBa_G0002
sequence annotation: BBa_G00000
sequence annotation: BBa_G00100
sequence annotation: BBa_B0055
sequence annotation: BBa_B0044
sequence annotation: BBa_B0062
sequence annotation: BBa_G00001
sequence annotation: BBa_I50042
sequence annotation: BBa_G00102
sequence annotation: BBa_B0043
sequence: https://synbiohub.org/public/igem/pSB4T5_sequence/1

BBa_B0053
type: Component
persistent identity: component2236456
display id: component2236456
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0053/1

BBa_G0003
type: Component
persistent identity: component2236421
display id: component2236421
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0003/1

BBa_B0055
type: Component
persistent identity: component2236461
display id: component2236461
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0055/1

BBa_B0042
type: Component
persistent identity: component2236468
display id: component2236468
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_P1005
type: Component
persistent identity: component2236453
display id: component2236453
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_P1005/1

BBa_B0043
type: Component
persistent identity: component2236473
display id: component2236473
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0043/1

BBa_G00100
type: Component
persistent identity: component2236458
display id: component2236458
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00100/1

BBa_G00001
type: Component
persistent identity: component2236401
display id: component2236401
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00001/1

BBa_B0062
type: Component
persistent identity: component2236420
display id: component2236420
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0062/1

BBa_B0044
type: Component
persistent identity: component2236406
display id: component2236406
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0044/1

BBa_G00102
type: Component
persistent identity: component2236418
display id: component2236418
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00102/1

BBa_B0054
type: Component
persistent identity: component2236416
display id: component2236416
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0054/1

BBa_G0002
type: Component
persistent identity: component2236450
display id: component2236450
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0002/1

BBa_B0042
type: Component
persistent identity: component2236413
display id: component2236413
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_I50042
type: Component
persistent identity: component2236449
display id: component2236449
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_I50042/1

BBa_G0004
type: Component
persistent identity: component2236454
display id: component2236454
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0004/1

BBa_G00000
type: Component
persistent identity: component2236478
display id: component2236478
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00000/1

BBa_G0003
type: SequenceAnnotation
persistent identity: annotation2236421
display id: annotation2236421
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236421/range2236421/1
component: BBa_G0003

https://synbiohub.org/public/igem/pSB4T5/annotation2236421/range2236421/1
type: Range
persistent identity: range2236421
display id: range2236421
version: 1
direction: direction/reverse
top level: pSB4T5
start: 177
end: 183
orientation: inline

BBa_G0004
type: SequenceAnnotation
persistent identity: annotation2236454
display id: annotation2236454
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236454/range2236454/1
component: BBa_G0004

https://synbiohub.org/public/igem/pSB4T5/annotation2236454/range2236454/1
type: Range
persistent identity: range2236454
display id: range2236454
version: 1
direction: direction/reverse
top level: pSB4T5
start: 3512
end: 3518
orientation: inline

BBa_B0053
type: SequenceAnnotation
persistent identity: annotation2236456
display id: annotation2236456
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236456/range2236456/1
component: BBa_B0053

https://synbiohub.org/public/igem/pSB4T5/annotation2236456/range2236456/1
type: Range
persistent identity: range2236456
display id: range2236456
version: 1
direction: direction/reverse
top level: pSB4T5
start: 3519
end: 3590
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2236413
display id: annotation2236413
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236413/range2236413/1
component: BBa_B0042

https://synbiohub.org/public/igem/pSB4T5/annotation2236413/range2236413/1
type: Range
persistent identity: range2236413
display id: range2236413
version: 1
direction: direction/reverse
top level: pSB4T5
start: 35
end: 46
orientation: inline

BBa_P1005
type: SequenceAnnotation
persistent identity: annotation2236453
display id: annotation2236453
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236453/range2236453/1
component: BBa_P1005

https://synbiohub.org/public/igem/pSB4T5/annotation2236453/range2236453/1
type: Range
persistent identity: range2236453
display id: range2236453
version: 1
direction: direction/reverse
top level: pSB4T5
start: 2229
end: 3511
orientation: inline

BBa_B0054
type: SequenceAnnotation
persistent identity: annotation2236416
display id: annotation2236416
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236416/range2236416/1
component: BBa_B0054

https://synbiohub.org/public/igem/pSB4T5/annotation2236416/range2236416/1
type: Range
persistent identity: range2236416
display id: range2236416
version: 1
direction: direction/reverse
top level: pSB4T5
start: 47
end: 115
orientation: inline

BBa_G0002
type: SequenceAnnotation
persistent identity: annotation2236450
display id: annotation2236450
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236450/range2236450/1
component: BBa_G0002

https://synbiohub.org/public/igem/pSB4T5/annotation2236450/range2236450/1
type: Range
persistent identity: range2236450
display id: range2236450
version: 1
direction: direction/reverse
top level: pSB4T5
start: 2221
end: 2228
orientation: inline

BBa_G00000
type: SequenceAnnotation
persistent identity: annotation2236478
display id: annotation2236478
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236478/range2236478/1
component: BBa_G00000

https://synbiohub.org/public/igem/pSB4T5/annotation2236478/range2236478/1
type: Range
persistent identity: range2236478
display id: range2236478
version: 1
direction: direction/reverse
top level: pSB4T5
start: 3714
end: 3735
orientation: inline

BBa_G00100
type: SequenceAnnotation
persistent identity: annotation2236458
display id: annotation2236458
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236458/range2236458/1
component: BBa_G00100

https://synbiohub.org/public/igem/pSB4T5/annotation2236458/range2236458/1
type: Range
persistent identity: range2236458
display id: range2236458
version: 1
direction: direction/reverse
top level: pSB4T5
start: 3591
end: 3610
orientation: inline

BBa_B0055
type: SequenceAnnotation
persistent identity: annotation2236461
display id: annotation2236461
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236461/range2236461/1
component: BBa_B0055

https://synbiohub.org/public/igem/pSB4T5/annotation2236461/range2236461/1
type: Range
persistent identity: range2236461
display id: range2236461
version: 1
direction: direction/reverse
top level: pSB4T5
start: 3611
end: 3688
orientation: inline

BBa_B0044
type: SequenceAnnotation
persistent identity: annotation2236406
display id: annotation2236406
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236406/range2236406/1
component: BBa_B0044

https://synbiohub.org/public/igem/pSB4T5/annotation2236406/range2236406/1
type: Range
persistent identity: range2236406
display id: range2236406
version: 1
direction: direction/reverse
top level: pSB4T5
start: 22
end: 34
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2236468
display id: annotation2236468
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236468/range2236468/1
component: BBa_B0042

https://synbiohub.org/public/igem/pSB4T5/annotation2236468/range2236468/1
type: Range
persistent identity: range2236468
display id: range2236468
version: 1
direction: direction/reverse
top level: pSB4T5
start: 3689
end: 3700
orientation: inline

BBa_B0062
type: SequenceAnnotation
persistent identity: annotation2236420
display id: annotation2236420
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236420/range2236420/1
component: BBa_B0062

https://synbiohub.org/public/igem/pSB4T5/annotation2236420/range2236420/1
type: Range
persistent identity: range2236420
display id: range2236420
version: 1
direction: direction/reverse
top level: pSB4T5
start: 136
end: 176
orientation: inline

BBa_G00001
type: SequenceAnnotation
persistent identity: annotation2236401
display id: annotation2236401
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236401/range2236401/1
component: BBa_G00001

https://synbiohub.org/public/igem/pSB4T5/annotation2236401/range2236401/1
type: Range
persistent identity: range2236401
display id: range2236401
version: 1
direction: direction/reverse
top level: pSB4T5
start: 1
end: 21
orientation: inline

BBa_I50042
type: SequenceAnnotation
persistent identity: annotation2236449
display id: annotation2236449
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236449/range2236449/1
component: BBa_I50042

https://synbiohub.org/public/igem/pSB4T5/annotation2236449/range2236449/1
type: Range
persistent identity: range2236449
display id: range2236449
version: 1
direction: direction/reverse
top level: pSB4T5
start: 184
end: 2220
orientation: inline

BBa_G00102
type: SequenceAnnotation
persistent identity: annotation2236418
display id: annotation2236418
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236418/range2236418/1
component: BBa_G00102

https://synbiohub.org/public/igem/pSB4T5/annotation2236418/range2236418/1
type: Range
persistent identity: range2236418
display id: range2236418
version: 1
direction: direction/reverse
top level: pSB4T5
start: 116
end: 135
orientation: inline

BBa_B0043
type: SequenceAnnotation
persistent identity: annotation2236473
display id: annotation2236473
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236473/range2236473/1
component: BBa_B0043

https://synbiohub.org/public/igem/pSB4T5/annotation2236473/range2236473/1
type: Range
persistent identity: range2236473
display id: range2236473
version: 1
direction: direction/reverse
top level: pSB4T5
start: 3701
end: 3713
orientation: inline

BBa_K864009
type: ComponentDefinition
persistent identity: BBa K864009
display id: BBa_K864009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector with LacI
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB4A15Iq is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and ampicillin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22. This vector carries a LacIq cassette on the backbone for tight repression of any lac promoter cloned into the cloning site.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone.
mutable provenance: Based on BBa_K864001
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Promoter
sequence annotation: -10
sequence annotation: RBS
sequence annotation: pSC101 ori
sequence annotation: P22 late terminator
sequence annotation: -35
sequence annotation: cmR
sequence annotation: lacI
sequence annotation: tonB terminator
sequence annotation: Lux terminator
sequence annotation: PlacIq promoter
sequence annotation: T3 terminator
sequence annotation: rrnC
sequence: https://synbiohub.org/public/igem/BBa_K864009_sequence/1

Promoter
type: SequenceAnnotation
persistent identity: annotation2202844
display id: annotation2202844
version: 1
top level: BBa_K864009
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K864009/annotation2202844/range2202844/1

https://synbiohub.org/public/igem/BBa_K864009/annotation2202844/range2202844/1
type: Range
persistent identity: range2202844
display id: range2202844
version: 1
direction: direction/reverse
top level: BBa_K864009
start: 4415
end: 4441
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2202857
display id: annotation2202857
version: 1
top level: BBa_K864009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864009/annotation2202857/range2202857/1

https://synbiohub.org/public/igem/BBa_K864009/annotation2202857/range2202857/1
type: Range
persistent identity: range2202857
display id: range2202857
version: 1
direction: direction/forward
top level: BBa_K864009
start: 2464
end: 2469
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2202848
display id: annotation2202848
version: 1
top level: BBa_K864009
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K864009/annotation2202848/range2202848/1

https://synbiohub.org/public/igem/BBa_K864009/annotation2202848/range2202848/1
type: Range
persistent identity: range2202848
display id: range2202848
version: 1
direction: direction/forward
top level: BBa_K864009
start: 2509
end: 2514
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2202856
display id: annotation2202856
version: 1
top level: BBa_K864009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864009/annotation2202856/range2202856/1

https://synbiohub.org/public/igem/BBa_K864009/annotation2202856/range2202856/1
type: Range
persistent identity: range2202856
display id: range2202856
version: 1
direction: direction/forward
top level: BBa_K864009
start: 903
end: 908
orientation: inline

pSC101 ori
type: SequenceAnnotation
persistent identity: annotation2202828
display id: annotation2202828
version: 1
top level: BBa_K864009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864009/annotation2202828/range2202828/1

https://synbiohub.org/public/igem/BBa_K864009/annotation2202828/range2202828/1
type: Range
persistent identity: range2202828
display id: range2202828
version: 1
direction: direction/forward
top level: BBa_K864009
start: 190
end: 2405
orientation: inline

P22 late terminator
type: SequenceAnnotation
persistent identity: annotation2202852
display id: annotation2202852
version: 1
top level: BBa_K864009
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K864009/annotation2202852/range2202852/1

https://synbiohub.org/public/igem/BBa_K864009/annotation2202852/range2202852/1
type: Range
persistent identity: range2202852
display id: range2202852
version: 1
direction: direction/forward
top level: BBa_K864009
start: 4461
end: 4501
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2202849
display id: annotation2202849
version: 1
top level: BBa_K864009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864009/annotation2202849/range2202849/1

https://synbiohub.org/public/igem/BBa_K864009/annotation2202849/range2202849/1
type: Range
persistent identity: range2202849
display id: range2202849
version: 1
direction: direction/forward
top level: BBa_K864009
start: 881
end: 886
orientation: inline

cmR
type: SequenceAnnotation
persistent identity: annotation2202847
display id: annotation2202847
version: 1
top level: BBa_K864009
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K864009/annotation2202847/range2202847/1

https://synbiohub.org/public/igem/BBa_K864009/annotation2202847/range2202847/1
type: Range
persistent identity: range2202847
display id: range2202847
version: 1
direction: direction/reverse
top level: BBa_K864009
start: 3686
end: 4348
orientation: inline

lacI
type: SequenceAnnotation
persistent identity: annotation2202846
display id: annotation2202846
version: 1
top level: BBa_K864009
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K864009/annotation2202846/range2202846/1

https://synbiohub.org/public/igem/BBa_K864009/annotation2202846/range2202846/1
type: Range
persistent identity: range2202846
display id: range2202846
version: 1
direction: direction/forward
top level: BBa_K864009
start: 2521
end: 3603
orientation: inline

tonB terminator
type: SequenceAnnotation
persistent identity: annotation2202854
display id: annotation2202854
version: 1
top level: BBa_K864009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864009/annotation2202854/range2202854/1

https://synbiohub.org/public/igem/BBa_K864009/annotation2202854/range2202854/1
type: Range
persistent identity: range2202854
display id: range2202854
version: 1
direction: direction/forward
top level: BBa_K864009
start: 47
end: 115
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2202850
display id: annotation2202850
version: 1
top level: BBa_K864009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864009/annotation2202850/range2202850/1

https://synbiohub.org/public/igem/BBa_K864009/annotation2202850/range2202850/1
type: Range
persistent identity: range2202850
display id: range2202850
version: 1
direction: direction/forward
top level: BBa_K864009
start: 2441
end: 2446
orientation: inline

Lux terminator
type: SequenceAnnotation
persistent identity: annotation2202851
display id: annotation2202851
version: 1
top level: BBa_K864009
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K864009/annotation2202851/range2202851/1

https://synbiohub.org/public/igem/BBa_K864009/annotation2202851/range2202851/1
type: Range
persistent identity: range2202851
display id: range2202851
version: 1
direction: direction/reverse
top level: BBa_K864009
start: 3634
end: 3679
orientation: inline

PlacIq promoter
type: SequenceAnnotation
persistent identity: annotation2202845
display id: annotation2202845
version: 1
top level: BBa_K864009
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K864009/annotation2202845/range2202845/1

https://synbiohub.org/public/igem/BBa_K864009/annotation2202845/range2202845/1
type: Range
persistent identity: range2202845
display id: range2202845
version: 1
direction: direction/forward
top level: BBa_K864009
start: 2439
end: 2494
orientation: inline

T3 terminator
type: SequenceAnnotation
persistent identity: annotation2202853
display id: annotation2202853
version: 1
top level: BBa_K864009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864009/annotation2202853/range2202853/1

https://synbiohub.org/public/igem/BBa_K864009/annotation2202853/range2202853/1
type: Range
persistent identity: range2202853
display id: range2202853
version: 1
direction: direction/forward
top level: BBa_K864009
start: 4522
end: 4599
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation2202855
display id: annotation2202855
version: 1
top level: BBa_K864009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864009/annotation2202855/range2202855/1

https://synbiohub.org/public/igem/BBa_K864009/annotation2202855/range2202855/1
type: Range
persistent identity: range2202855
display id: range2202855
version: 1
direction: direction/forward
top level: BBa_K864009
start: 136
end: 175
orientation: inline

pSB8C15
type: ComponentDefinition
persistent identity: BBa K864017
display id: BBa_K864017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick temperature sensitive standard vector
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB8C15 is a BioBrick standard vector with low copy pSC101ts replication origin (~5 copies per cell) and Chloramphenicol antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The pSC101ts is a temperature sensitive pSC101 repelication origin and propagates at 30???C but stop replicating at 42???C. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone. The pSC101ts origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
mutable provenance: Based on BBa_K864001
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: cmR
sequence annotation: -35
sequence annotation: -10
sequence annotation: Lux terminator
sequence annotation: VR reverse primer binding site
sequence annotation: T3 terminator
sequence annotation: Promoter
sequence annotation: VF2 forward primer binding site
sequence annotation: tonB terminator
sequence annotation: rrnC
sequence annotation: P22 late terminator
sequence annotation: pSC101-ts ori
sequence: https://synbiohub.org/public/igem/BBa_K864017_sequence/1

cmR
type: SequenceAnnotation
persistent identity: annotation2203155
display id: annotation2203155
version: 1
top level: pSB8C15
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K864017/annotation2203155/range2203155/1

https://synbiohub.org/public/igem/BBa_K864017/annotation2203155/range2203155/1
type: Range
persistent identity: range2203155
display id: range2203155
version: 1
direction: direction/reverse
top level: pSB8C15
start: 2506
end: 3168
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2203156
display id: annotation2203156
version: 1
top level: pSB8C15
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864017/annotation2203156/range2203156/1

https://synbiohub.org/public/igem/BBa_K864017/annotation2203156/range2203156/1
type: Range
persistent identity: range2203156
display id: range2203156
version: 1
direction: direction/forward
top level: pSB8C15
start: 881
end: 886
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2203162
display id: annotation2203162
version: 1
top level: pSB8C15
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864017/annotation2203162/range2203162/1

https://synbiohub.org/public/igem/BBa_K864017/annotation2203162/range2203162/1
type: Range
persistent identity: range2203162
display id: range2203162
version: 1
direction: direction/forward
top level: pSB8C15
start: 903
end: 908
orientation: inline

Lux terminator
type: SequenceAnnotation
persistent identity: annotation2203159
display id: annotation2203159
version: 1
top level: pSB8C15
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864017/annotation2203159/range2203159/1

https://synbiohub.org/public/igem/BBa_K864017/annotation2203159/range2203159/1
type: Range
persistent identity: range2203159
display id: range2203159
version: 1
direction: direction/reverse
top level: pSB8C15
start: 2454
end: 2499
orientation: inline

VR reverse primer binding site
type: SequenceAnnotation
persistent identity: annotation2203046
display id: annotation2203046
version: 1
top level: pSB8C15
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864017/annotation2203046/range2203046/1

https://synbiohub.org/public/igem/BBa_K864017/annotation2203046/range2203046/1
type: Range
persistent identity: range2203046
display id: range2203046
version: 1
direction: direction/reverse
top level: pSB8C15
start: 116
end: 135
orientation: inline

T3 terminator
type: SequenceAnnotation
persistent identity: annotation2203161
display id: annotation2203161
version: 1
top level: pSB8C15
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864017/annotation2203161/range2203161/1

https://synbiohub.org/public/igem/BBa_K864017/annotation2203161/range2203161/1
type: Range
persistent identity: range2203161
display id: range2203161
version: 1
direction: direction/forward
top level: pSB8C15
start: 3342
end: 3419
orientation: inline

Promoter
type: SequenceAnnotation
persistent identity: annotation2202888
display id: annotation2202888
version: 1
top level: pSB8C15
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K864017/annotation2202888/range2202888/1

https://synbiohub.org/public/igem/BBa_K864017/annotation2202888/range2202888/1
type: Range
persistent identity: range2202888
display id: range2202888
version: 1
direction: direction/reverse
top level: pSB8C15
start: 3235
end: 3461
orientation: inline

VF2 forward primer binding site
type: SequenceAnnotation
persistent identity: annotation2203154
display id: annotation2203154
version: 1
top level: pSB8C15
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864017/annotation2203154/range2203154/1

https://synbiohub.org/public/igem/BBa_K864017/annotation2203154/range2203154/1
type: Range
persistent identity: range2203154
display id: range2203154
version: 1
direction: direction/forward
top level: pSB8C15
start: 3322
end: 3341
orientation: inline

tonB terminator
type: SequenceAnnotation
persistent identity: annotation2203157
display id: annotation2203157
version: 1
top level: pSB8C15
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K864017/annotation2203157/range2203157/1

https://synbiohub.org/public/igem/BBa_K864017/annotation2203157/range2203157/1
type: Range
persistent identity: range2203157
display id: range2203157
version: 1
direction: direction/forward
top level: pSB8C15
start: 47
end: 115
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation2203158
display id: annotation2203158
version: 1
top level: pSB8C15
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864017/annotation2203158/range2203158/1

https://synbiohub.org/public/igem/BBa_K864017/annotation2203158/range2203158/1
type: Range
persistent identity: range2203158
display id: range2203158
version: 1
direction: direction/forward
top level: pSB8C15
start: 136
end: 175
orientation: inline

P22 late terminator
type: SequenceAnnotation
persistent identity: annotation2203160
display id: annotation2203160
version: 1
top level: pSB8C15
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864017/annotation2203160/range2203160/1

https://synbiohub.org/public/igem/BBa_K864017/annotation2203160/range2203160/1
type: Range
persistent identity: range2203160
display id: range2203160
version: 1
direction: direction/forward
top level: pSB8C15
start: 3281
end: 3321
orientation: inline

pSC101-ts ori
type: SequenceAnnotation
persistent identity: annotation2203153
display id: annotation2203153
version: 1
top level: pSB8C15
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864017/annotation2203153/range2203153/1

https://synbiohub.org/public/igem/BBa_K864017/annotation2203153/range2203153/1
type: Range
persistent identity: range2203153
display id: range2203153
version: 1
direction: direction/forward
top level: pSB8C15
start: 190
end: 2405
orientation: inline

BBa_K864014
type: ComponentDefinition
persistent identity: BBa K864014
display id: BBa_K864014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector with LacI
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB4A15Iq(FRT) is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and ampicillin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22. This vector carries a LacIq cassette on the backbone for tight repression of any lac promoter cloned into the cloning site.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone.
mutable provenance: Based on BBa_K864006
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K864014_sequence/1

pSB8A15
type: ComponentDefinition
persistent identity: BBa K864016
display id: BBa_K864016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick temperature sensitive standard vector
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB8A15 is a BioBrick standard vector with low copy pSC101ts replication origin (~5 copies per cell) and ampicillin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The pSC101ts is a temperature sensitive pSC101 repelication origin and propagates at 30???C but stop replicating at 42???C. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone. The pSC101ts origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
mutable provenance: Based on BBa_K864000
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K864016_sequence/1

BBa_K864015
type: ComponentDefinition
persistent identity: BBa K864015
display id: BBa_K864015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector with LacI
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB4S15Iq(FRT) is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and spectinomycin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22. This vector carries a LacIq cassette on the backbone for tight repression of any lac promoter cloned into the cloning site.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone.
mutable provenance: Based on BBa_K864007
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K864015_sequence/1

BBa_I51019
type: ComponentDefinition
persistent identity: BBa I51019
display id: BBa_I51019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BioBrick base vector, as synthesized by Codon Devices
creator: Reshma Shetty
created: 2008-03-22T12:00:00Z
modified: 2015-08-31T04:07:42Z
discontinued: true
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: BBa_I51019 is the sequence of the BioBrick base vector as synthesized by Codon Devices, Inc. in Cambridge, MA. The original design of the BioBrick base vector is available as BBa_I51000. BBa_I51001 is the sequence of the BioBrick base vector as reported by Codon Devices, Inc. although the true sequence is something like to BBa_I51019. The exact sequence of the provided DNA is difficult to determine. The corrected BioBrick base vector that was successfully constructed from BBa_I51019 is BBa_I51020.
mutable notes: Synthesis of the BioBrick base vector design (BBa_I51000) was problematic. We encountered two issues while trying to construct the base vector. First, troubleshooting efforts during synthesis compromised the design of our parts: failed attempts to clone the base vector into an ''E. coli'' strain intolerant of expression of the toxic protein CcdB led to an unnecessary redesign of the ''ccdB'' positive selection marker in the BioBrick base vector. As a result, a revised design for the BioBrick base vector (BBa_I51001) was specified instead. Second, faulty part design adversely impacted the synthesis process: our pUC19-based replication origin design (BBa_I50020) was similarly nonfunctional, so the revised base vector (BBa_I51001) could not be propagated as specified. We eventually determined that the provided DNA for the BioBrick base vector was actually a fusion of two slightly different copies of the base vector: one with a nonfunctional version of the pUC19 origin (BBa_I50020) and one with a functional version of the pUC19 origin (BBa_I50022). We corrected the synthesized BioBrick base vector (BBa_I51019) with molecular biology techniques to make the functional BioBrick base vector BBa_I51020 (see Methods). Commercial DNA synthesis processes currently rely on cloning, assembly and propagation of synthesized DNA in ''E. coli''. Difficulties during synthesis stemmed from the inclusion of both a ''ccdB'' positive selection marker that is toxic to most ''E. coli'' strains and a synthetic origin incapable of supporting plasmid replication of the BioBrick base vector. In general, for parts whose function are incompatible with growth and replication of ''E. coli'', the processes of DNA design and DNA synthesis cannot be easily decoupled.
mutable provenance: BBa_I51001
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
component: BBa_B0055
component: BBa_B0053
component: BBa_G00001
component: BBa_P1006
component: BBa_B0045
component: BBa_B0044
component: BBa_B0043
component: BBa_G00000
component: BBa_B0042
component: BBa_B0054
component: BBa_G00102
component: BBa_G00100
component: BBa_I50022
component: BBa_B0062
component: BBa_P1016
component: BBa_I50020
sequence annotation: BBa_B0053
sequence annotation: BBa_B0045
sequence annotation: BBa_B0044
sequence annotation: BBa_B0054
sequence annotation: BBa_B0043
sequence annotation: BBa_G00000
sequence annotation: BBa_B0055
sequence annotation: BBa_B0062
sequence annotation: BBa_B0042
sequence annotation: BBa_G00100

BBa_B0055
type: Component
persistent identity: component1961891
display id: component1961891
version: 1
top level: BBa_I51019
access: public
definition: https://synbiohub.org/public/igem/BBa_B0055/1

BBa_B0053
type: Component
persistent identity: component1961886
display id: component1961886
version: 1
top level: BBa_I51019
access: public
definition: https://synbiohub.org/public/igem/BBa_B0053/1

BBa_G00001
type: Component
persistent identity: component1961856
display id: component1961856
version: 1
top level: BBa_I51019
access: public
definition: https://synbiohub.org/public/igem/BBa_G00001/1

BBa_P1006
type: Component
persistent identity: component1961958
display id: component1961958
version: 1
top level: BBa_I51019
access: public
definition: https://synbiohub.org/public/igem/BBa_P1006/1

BBa_B0045
type: Component
persistent identity: component1961953
display id: component1961953
version: 1
top level: BBa_I51019
access: public
definition: https://synbiohub.org/public/igem/BBa_B0045/1

BBa_B0044
type: Component
persistent identity: component1961937
display id: component1961937
version: 1
top level: BBa_I51019
access: public
definition: https://synbiohub.org/public/igem/BBa_B0044/1

BBa_B0053
type: Component
persistent identity: component1961962
display id: component1961962
version: 1
top level: BBa_I51019
access: public
definition: https://synbiohub.org/public/igem/BBa_B0053/1

BBa_B0043
type: Component
persistent identity: component1961903
display id: component1961903
version: 1
top level: BBa_I51019
access: public
definition: https://synbiohub.org/public/igem/BBa_B0043/1

BBa_G00000
type: Component
persistent identity: component1961984
display id: component1961984
version: 1
top level: BBa_I51019
access: public
definition: https://synbiohub.org/public/igem/BBa_G00000/1

BBa_B0042
type: Component
persistent identity: component1961944
display id: component1961944
version: 1
top level: BBa_I51019
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_B0043
type: Component
persistent identity: component1961979
display id: component1961979
version: 1
top level: BBa_I51019
access: public
definition: https://synbiohub.org/public/igem/BBa_B0043/1

BBa_B0045
type: Component
persistent identity: component1961877
display id: component1961877
version: 1
top level: BBa_I51019
access: public
definition: https://synbiohub.org/public/igem/BBa_B0045/1

BBa_B0054
type: Component
persistent identity: component1961947
display id: component1961947
version: 1
top level: BBa_I51019
access: public
definition: https://synbiohub.org/public/igem/BBa_B0054/1

BBa_B0045
type: Component
persistent identity: component1961960
display id: component1961960
version: 1
top level: BBa_I51019
access: public
definition: https://synbiohub.org/public/igem/BBa_B0045/1

BBa_G00102
type: Component
persistent identity: component1961949
display id: component1961949
version: 1
top level: BBa_I51019
access: public
definition: https://synbiohub.org/public/igem/BBa_G00102/1

BBa_G00100
type: Component
persistent identity: component1961888
display id: component1961888
version: 1
top level: BBa_I51019
access: public
definition: https://synbiohub.org/public/igem/BBa_G00100/1

BBa_G00001
type: Component
persistent identity: component1961932
display id: component1961932
version: 1
top level: BBa_I51019
access: public
definition: https://synbiohub.org/public/igem/BBa_G00001/1

BBa_B0045
type: Component
persistent identity: component1961884
display id: component1961884
version: 1
top level: BBa_I51019
access: public
definition: https://synbiohub.org/public/igem/BBa_B0045/1

BBa_B0044
type: Component
persistent identity: component1961861
display id: component1961861
version: 1
top level: BBa_I51019
access: public
definition: https://synbiohub.org/public/igem/BBa_B0044/1

BBa_G00000
type: Component
persistent identity: component1961908
display id: component1961908
version: 1
top level: BBa_I51019
access: public
definition: https://synbiohub.org/public/igem/BBa_G00000/1

BBa_B0054
type: Component
persistent identity: component1961871
display id: component1961871
version: 1
top level: BBa_I51019
access: public
definition: https://synbiohub.org/public/igem/BBa_B0054/1

BBa_P1006
type: Component
persistent identity: component1961882
display id: component1961882
version: 1
top level: BBa_I51019
access: public
definition: https://synbiohub.org/public/igem/BBa_P1006/1

BBa_I50022
type: Component
persistent identity: component1961998
display id: component1961998
version: 1
top level: BBa_I51019
access: public
definition: https://synbiohub.org/public/igem/BBa_I50022/1

BBa_B0062
type: Component
persistent identity: component1961875
display id: component1961875
version: 1
top level: BBa_I51019
access: public
definition: https://synbiohub.org/public/igem/BBa_B0062/1

BBa_P1016
type: Component
persistent identity: component1961988
display id: component1961988
version: 1
top level: BBa_I51019
access: public
definition: https://synbiohub.org/public/igem/BBa_P1016/1

BBa_B0062
type: Component
persistent identity: component1961951
display id: component1961951
version: 1
top level: BBa_I51019
access: public
definition: https://synbiohub.org/public/igem/BBa_B0062/1

BBa_B0042
type: Component
persistent identity: component1961898
display id: component1961898
version: 1
top level: BBa_I51019
access: public
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BBa_B0043
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BBa_G00000
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BBa_B0062
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BBa_B0042
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BBa_G00100
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BBa_G00000
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BBa_B0042
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BBa_P1016
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BBa_B0045
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BBa_G00001
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BBa_P1006
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BBa_I50020
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persistent identity: annotation1961927
display id: annotation1961927
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display id: range1961927
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BBa_B0062
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display id: annotation1961875
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location: https://synbiohub.org/public/igem/BBa_I51019/annotation1961875/range1961875/1
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display id: range1961875
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BBa_B0045
type: SequenceAnnotation
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display id: annotation1961953
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persistent identity: range1961953
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BBa_G00001
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display id: annotation1961932
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BBa_G00102
type: SequenceAnnotation
persistent identity: annotation1961873
display id: annotation1961873
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BBa_P1006
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display id: annotation1961882
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display id: range1961882
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BBa_I50022
type: SequenceAnnotation
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BBa_G00100
type: SequenceAnnotation
persistent identity: annotation1961964
display id: annotation1961964
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BBa_G00102
type: SequenceAnnotation
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version: 1
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pSB8S15FRT
type: ComponentDefinition
persistent identity: BBa K864023
display id: BBa_K864023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick temperature sensitive standard vector
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB8S15(FRT) is a BioBrick standard vector with low copy pSC101ts replication origin (~5 copies per cell) and spectinomycin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The pSC101ts is a temperature sensitive pSC101 repelication origin and propagates at 30???C but stop replicating at 42???C. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone. The pSC101ts origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
mutable provenance: Based on BBa_K864017
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K864023_sequence/1

BBa_K864001
type: ComponentDefinition
persistent identity: BBa K864001
display id: BBa_K864001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector
creator: Erik Lundin
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB4C15 is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and chloramphenicol antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone. The pSC101 origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
mutable provenance: Based on pSB3T5
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: pSC101 ori
sequence annotation: Tandem repeat
sequence annotation: repA
sequence annotation: CmR BBa_P1004
sequence annotation: -10
sequence annotation: Lambda Red reverse primer
sequence annotation: VR
sequence annotation: Lux terminator
sequence annotation: Mutated A>T to remove SpeI
sequence annotation: P22 late terminator
sequence annotation: T3 terminator
sequence annotation: Lambda Red forward primer
sequence annotation: Repeat "4"
sequence annotation: Repeat "5"
sequence annotation: VF2
sequence annotation: -35
sequence annotation: rrnC terminator
sequence annotation: Promoter
sequence annotation: tonB terminator
sequence: https://synbiohub.org/public/igem/BBa_K864001_sequence/1

pSC101 ori
type: SequenceAnnotation
persistent identity: annotation2198568
display id: annotation2198568
version: 1
top level: BBa_K864001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198568/range2198568/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198568/range2198568/1
type: Range
persistent identity: range2198568
display id: range2198568
version: 1
direction: direction/forward
top level: BBa_K864001
start: 190
end: 2405
orientation: inline

Tandem repeat
type: SequenceAnnotation
persistent identity: annotation2198578
display id: annotation2198578
version: 1
top level: BBa_K864001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198578/range2198578/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198578/range2198578/1
type: Range
persistent identity: range2198578
display id: range2198578
version: 1
direction: direction/forward
top level: BBa_K864001
start: 820
end: 841
orientation: inline

repA
type: SequenceAnnotation
persistent identity: annotation2198605
display id: annotation2198605
version: 1
top level: BBa_K864001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198605/range2198605/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198605/range2198605/1
type: Range
persistent identity: range2198605
display id: range2198605
version: 1
direction: direction/forward
top level: BBa_K864001
start: 939
end: 1889
orientation: inline

CmR BBa_P1004
type: SequenceAnnotation
persistent identity: annotation2198620
display id: annotation2198620
version: 1
top level: BBa_K864001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198620/range2198620/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198620/range2198620/1
type: Range
persistent identity: range2198620
display id: range2198620
version: 1
direction: direction/reverse
top level: BBa_K864001
start: 2506
end: 3168
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2198603
display id: annotation2198603
version: 1
top level: BBa_K864001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198603/range2198603/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198603/range2198603/1
type: Range
persistent identity: range2198603
display id: range2198603
version: 1
direction: direction/forward
top level: BBa_K864001
start: 903
end: 908
orientation: inline

Tandem repeat
type: SequenceAnnotation
persistent identity: annotation2198576
display id: annotation2198576
version: 1
top level: BBa_K864001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198576/range2198576/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198576/range2198576/1
type: Range
persistent identity: range2198576
display id: range2198576
version: 1
direction: direction/forward
top level: BBa_K864001
start: 790
end: 809
orientation: inline

Lambda Red reverse primer
type: SequenceAnnotation
persistent identity: annotation2198340
display id: annotation2198340
version: 1
top level: BBa_K864001
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198340/range2198340/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198340/range2198340/1
type: Range
persistent identity: range2198340
display id: range2198340
version: 1
direction: direction/reverse
top level: BBa_K864001
start: 18
end: 38
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation2198565
display id: annotation2198565
version: 1
top level: BBa_K864001
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198565/range2198565/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198565/range2198565/1
type: Range
persistent identity: range2198565
display id: range2198565
version: 1
direction: direction/reverse
top level: BBa_K864001
start: 116
end: 135
orientation: inline

Lux terminator
type: SequenceAnnotation
persistent identity: annotation2198616
display id: annotation2198616
version: 1
top level: BBa_K864001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198616/range2198616/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198616/range2198616/1
type: Range
persistent identity: range2198616
display id: range2198616
version: 1
direction: direction/reverse
top level: BBa_K864001
start: 2454
end: 2499
orientation: inline

Mutated A>T to remove SpeI
type: SequenceAnnotation
persistent identity: annotation2198582
display id: annotation2198582
version: 1
top level: BBa_K864001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198582/range2198582/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198582/range2198582/1
type: Range
persistent identity: range2198582
display id: range2198582
version: 1
direction: direction/forward
top level: BBa_K864001
start: 867
end: 867
orientation: inline

P22 late terminator
type: SequenceAnnotation
persistent identity: annotation2198628
display id: annotation2198628
version: 1
top level: BBa_K864001
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198628/range2198628/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198628/range2198628/1
type: Range
persistent identity: range2198628
display id: range2198628
version: 1
direction: direction/forward
top level: BBa_K864001
start: 3251
end: 3321
orientation: inline

T3 terminator
type: SequenceAnnotation
persistent identity: annotation2198614
display id: annotation2198614
version: 1
top level: BBa_K864001
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198614/range2198614/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198614/range2198614/1
type: Range
persistent identity: range2198614
display id: range2198614
version: 1
direction: direction/forward
top level: BBa_K864001
start: 3342
end: 3419
orientation: inline

Lambda Red forward primer
type: SequenceAnnotation
persistent identity: annotation2198606
display id: annotation2198606
version: 1
top level: BBa_K864001
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198606/range2198606/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198606/range2198606/1
type: Range
persistent identity: range2198606
display id: range2198606
version: 1
direction: direction/forward
top level: BBa_K864001
start: 2433
end: 2450
orientation: inline

Repeat "4"
type: SequenceAnnotation
persistent identity: annotation2198580
display id: annotation2198580
version: 1
top level: BBa_K864001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198580/range2198580/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198580/range2198580/1
type: Range
persistent identity: range2198580
display id: range2198580
version: 1
direction: direction/forward
top level: BBa_K864001
start: 862
end: 880
orientation: inline

Repeat "5"
type: SequenceAnnotation
persistent identity: annotation2198585
display id: annotation2198585
version: 1
top level: BBa_K864001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198585/range2198585/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198585/range2198585/1
type: Range
persistent identity: range2198585
display id: range2198585
version: 1
direction: direction/forward
top level: BBa_K864001
start: 891
end: 912
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation2198629
display id: annotation2198629
version: 1
top level: BBa_K864001
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198629/range2198629/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198629/range2198629/1
type: Range
persistent identity: range2198629
display id: range2198629
version: 1
direction: direction/forward
top level: BBa_K864001
start: 3322
end: 3341
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2198584
display id: annotation2198584
version: 1
top level: BBa_K864001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198584/range2198584/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198584/range2198584/1
type: Range
persistent identity: range2198584
display id: range2198584
version: 1
direction: direction/forward
top level: BBa_K864001
start: 881
end: 886
orientation: inline

rrnC terminator
type: SequenceAnnotation
persistent identity: annotation2198566
display id: annotation2198566
version: 1
top level: BBa_K864001
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198566/range2198566/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198566/range2198566/1
type: Range
persistent identity: range2198566
display id: range2198566
version: 1
direction: direction/forward
top level: BBa_K864001
start: 136
end: 175
orientation: inline

Promoter
type: SequenceAnnotation
persistent identity: annotation2198622
display id: annotation2198622
version: 1
top level: BBa_K864001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198622/range2198622/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198622/range2198622/1
type: Range
persistent identity: range2198622
display id: range2198622
version: 1
direction: direction/reverse
top level: BBa_K864001
start: 3235
end: 3261
orientation: inline

tonB terminator
type: SequenceAnnotation
persistent identity: annotation2198561
display id: annotation2198561
version: 1
top level: BBa_K864001
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198561/range2198561/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198561/range2198561/1
type: Range
persistent identity: range2198561
display id: range2198561
version: 1
direction: direction/forward
top level: BBa_K864001
start: 47
end: 115
orientation: inline

Tandem repeat
type: SequenceAnnotation
persistent identity: annotation2198574
display id: annotation2198574
version: 1
top level: BBa_K864001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198574/range2198574/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198574/range2198574/1
type: Range
persistent identity: range2198574
display id: range2198574
version: 1
direction: direction/forward
top level: BBa_K864001
start: 767
end: 788
orientation: inline

pSB8K15
type: ComponentDefinition
persistent identity: BBa K864018
display id: BBa_K864018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick temperature sensitive standard vector
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB8K15 is a BioBrick standard vector with low copy pSC101ts replication origin (~5 copies per cell) and ampicillin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The pSC101ts is a temperature sensitive pSC101 repelication origin and propagates at 30???C but stop replicating at 42???C. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone. The pSC101ts origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
mutable provenance: Based on BBa_K864002
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K864018_sequence/1

BBa_K864002
type: ComponentDefinition
persistent identity: BBa K864002
display id: BBa_K864002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector
creator: Erik Lundin
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB4K15 is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and kanamycin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone. The pSC101 origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
mutable provenance: Based on pSB3T5
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: rrnC terminator
sequence annotation: P22 late terminator
sequence annotation: KanR BBa_P1003
sequence annotation: pSC101 ori BBa_K864050
sequence annotation: Lux terminator
sequence annotation: Lambda Red forward primer
sequence annotation: TonB terminator
sequence annotation: VF2
sequence annotation: Lambda Red reverse primer
sequence annotation: T3 terminator
sequence annotation: VR
sequence: https://synbiohub.org/public/igem/BBa_K864002_sequence/1

rrnC terminator
type: SequenceAnnotation
persistent identity: annotation2198834
display id: annotation2198834
version: 1
top level: BBa_K864002
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864002/annotation2198834/range2198834/1

https://synbiohub.org/public/igem/BBa_K864002/annotation2198834/range2198834/1
type: Range
persistent identity: range2198834
display id: range2198834
version: 1
direction: direction/forward
top level: BBa_K864002
start: 136
end: 175
orientation: inline

P22 late terminator
type: SequenceAnnotation
persistent identity: annotation2198839
display id: annotation2198839
version: 1
top level: BBa_K864002
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864002/annotation2198839/range2198839/1

https://synbiohub.org/public/igem/BBa_K864002/annotation2198839/range2198839/1
type: Range
persistent identity: range2198839
display id: range2198839
version: 1
direction: direction/forward
top level: BBa_K864002
start: 3478
end: 3518
orientation: inline

KanR BBa_P1003
type: SequenceAnnotation
persistent identity: annotation2198838
display id: annotation2198838
version: 1
top level: BBa_K864002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864002/annotation2198838/range2198838/1

https://synbiohub.org/public/igem/BBa_K864002/annotation2198838/range2198838/1
type: Range
persistent identity: range2198838
display id: range2198838
version: 1
direction: direction/forward
top level: BBa_K864002
start: 2506
end: 3224
orientation: inline

pSC101 ori BBa_K864050
type: SequenceAnnotation
persistent identity: annotation2198835
display id: annotation2198835
version: 1
top level: BBa_K864002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864002/annotation2198835/range2198835/1

https://synbiohub.org/public/igem/BBa_K864002/annotation2198835/range2198835/1
type: Range
persistent identity: range2198835
display id: range2198835
version: 1
direction: direction/forward
top level: BBa_K864002
start: 190
end: 2405
orientation: inline

Lux terminator
type: SequenceAnnotation
persistent identity: annotation2198837
display id: annotation2198837
version: 1
top level: BBa_K864002
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864002/annotation2198837/range2198837/1

https://synbiohub.org/public/igem/BBa_K864002/annotation2198837/range2198837/1
type: Range
persistent identity: range2198837
display id: range2198837
version: 1
direction: direction/reverse
top level: BBa_K864002
start: 2454
end: 2499
orientation: inline

Lambda Red forward primer
type: SequenceAnnotation
persistent identity: annotation2198836
display id: annotation2198836
version: 1
top level: BBa_K864002
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864002/annotation2198836/range2198836/1

https://synbiohub.org/public/igem/BBa_K864002/annotation2198836/range2198836/1
type: Range
persistent identity: range2198836
display id: range2198836
version: 1
direction: direction/forward
top level: BBa_K864002
start: 2433
end: 2450
orientation: inline

TonB terminator
type: SequenceAnnotation
persistent identity: annotation2198832
display id: annotation2198832
version: 1
top level: BBa_K864002
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864002/annotation2198832/range2198832/1

https://synbiohub.org/public/igem/BBa_K864002/annotation2198832/range2198832/1
type: Range
persistent identity: range2198832
display id: range2198832
version: 1
direction: direction/forward
top level: BBa_K864002
start: 47
end: 115
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation2198840
display id: annotation2198840
version: 1
top level: BBa_K864002
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864002/annotation2198840/range2198840/1

https://synbiohub.org/public/igem/BBa_K864002/annotation2198840/range2198840/1
type: Range
persistent identity: range2198840
display id: range2198840
version: 1
direction: direction/forward
top level: BBa_K864002
start: 3519
end: 3538
orientation: inline

Lambda Red reverse primer
type: SequenceAnnotation
persistent identity: annotation2198831
display id: annotation2198831
version: 1
top level: BBa_K864002
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864002/annotation2198831/range2198831/1

https://synbiohub.org/public/igem/BBa_K864002/annotation2198831/range2198831/1
type: Range
persistent identity: range2198831
display id: range2198831
version: 1
direction: direction/reverse
top level: BBa_K864002
start: 18
end: 38
orientation: inline

T3 terminator
type: SequenceAnnotation
persistent identity: annotation2198841
display id: annotation2198841
version: 1
top level: BBa_K864002
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864002/annotation2198841/range2198841/1

https://synbiohub.org/public/igem/BBa_K864002/annotation2198841/range2198841/1
type: Range
persistent identity: range2198841
display id: range2198841
version: 1
direction: direction/forward
top level: BBa_K864002
start: 3539
end: 3616
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation2198833
display id: annotation2198833
version: 1
top level: BBa_K864002
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864002/annotation2198833/range2198833/1

https://synbiohub.org/public/igem/BBa_K864002/annotation2198833/range2198833/1
type: Range
persistent identity: range2198833
display id: range2198833
version: 1
direction: direction/forward
top level: BBa_K864002
start: 116
end: 135
orientation: inline

pSB4K5
type: ComponentDefinition
persistent identity: pSB4K5
display id: pSB4K5
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector
creator: Reshma Shetty
created: 2007-02-28T12:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: pSB4K5 is a low copy plasmid with kanamycin resistance. It has a repA pSC101-derived replication origin. (Copy number ~10-12, Lutz and Bujard, 1997.) It is derived from the BioBricks vector scaffold BBa_I51001.
mutable notes: See http://openwetware.org/wiki/Synthetic_Biology:Vectors/pSB%2A%2A5_design for more information.
mutable provenance: BBa_I51001 vector scaffold, BBa_I50040 replication origin and BBa_P1003 antibiotic resistance marker.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
component: BBa_G0006
component: BBa_G00100
component: BBa_B0062
component: BBa_G0005
component: BBa_G00102
component: BBa_B0043
component: BBa_G0001
component: BBa_B0044
component: BBa_I50041
component: BBa_B0055
component: BBa_B0042
component: BBa_G00001
component: BBa_B0053
component: BBa_P1007
component: BBa_G00000
component: BBa_B0054
sequence annotation: BBa_B0054
sequence annotation: BBa_G0005
sequence annotation: BBa_B0044
sequence annotation: BBa_B0042
sequence annotation: BBa_P1007
sequence annotation: BBa_G00000
sequence annotation: BBa_G00100
sequence annotation: BBa_G00001
sequence annotation: BBa_B0053
sequence annotation: BBa_G0001
sequence annotation: BBa_G0006
sequence annotation: BBa_G00102
sequence annotation: BBa_I50041
sequence annotation: BBa_B0062
sequence annotation: BBa_B0043
sequence annotation: BBa_B0055
sequence: https://synbiohub.org/public/igem/pSB4K5_sequence/1

BBa_G0006
type: Component
persistent identity: component2236031
display id: component2236031
version: 1
top level: pSB4K5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0006/1

BBa_G00100
type: Component
persistent identity: component2236035
display id: component2236035
version: 1
top level: pSB4K5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00100/1

BBa_B0062
type: Component
persistent identity: component2235998
display id: component2235998
version: 1
top level: pSB4K5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0062/1

BBa_G0005
type: Component
persistent identity: component2235999
display id: component2235999
version: 1
top level: pSB4K5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0005/1

BBa_G00102
type: Component
persistent identity: component2235996
display id: component2235996
version: 1
top level: pSB4K5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00102/1

BBa_B0043
type: Component
persistent identity: component2236050
display id: component2236050
version: 1
top level: pSB4K5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0043/1

BBa_G0001
type: Component
persistent identity: component2236004
display id: component2236004
version: 1
top level: pSB4K5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0001/1

BBa_B0044
type: Component
persistent identity: component2235984
display id: component2235984
version: 1
top level: pSB4K5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0044/1

BBa_I50041
type: Component
persistent identity: component2236030
display id: component2236030
version: 1
top level: pSB4K5
access: public
definition: https://synbiohub.org/public/igem/BBa_I50041/1

BBa_B0055
type: Component
persistent identity: component2236038
display id: component2236038
version: 1
top level: pSB4K5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0055/1

BBa_B0042
type: Component
persistent identity: component2236045
display id: component2236045
version: 1
top level: pSB4K5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_G00001
type: Component
persistent identity: component2235979
display id: component2235979
version: 1
top level: pSB4K5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00001/1

BBa_B0053
type: Component
persistent identity: component2236033
display id: component2236033
version: 1
top level: pSB4K5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0053/1

BBa_P1007
type: Component
persistent identity: component2236003
display id: component2236003
version: 1
top level: pSB4K5
access: public
definition: https://synbiohub.org/public/igem/BBa_P1007/1

BBa_B0042
type: Component
persistent identity: component2235991
display id: component2235991
version: 1
top level: pSB4K5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_G00000
type: Component
persistent identity: component2236055
display id: component2236055
version: 1
top level: pSB4K5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00000/1

BBa_B0054
type: Component
persistent identity: component2235994
display id: component2235994
version: 1
top level: pSB4K5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0054/1

BBa_B0054
type: SequenceAnnotation
persistent identity: annotation2235994
display id: annotation2235994
version: 1
top level: pSB4K5
location: https://synbiohub.org/public/igem/pSB4K5/annotation2235994/range2235994/1
component: BBa_B0054

https://synbiohub.org/public/igem/pSB4K5/annotation2235994/range2235994/1
type: Range
persistent identity: range2235994
display id: range2235994
version: 1
direction: direction/reverse
top level: pSB4K5
start: 47
end: 115
orientation: inline

BBa_G0005
type: SequenceAnnotation
persistent identity: annotation2235999
display id: annotation2235999
version: 1
top level: pSB4K5
location: https://synbiohub.org/public/igem/pSB4K5/annotation2235999/range2235999/1
component: BBa_G0005

https://synbiohub.org/public/igem/pSB4K5/annotation2235999/range2235999/1
type: Range
persistent identity: range2235999
display id: range2235999
version: 1
direction: direction/reverse
top level: pSB4K5
start: 177
end: 183
orientation: inline

BBa_B0044
type: SequenceAnnotation
persistent identity: annotation2235984
display id: annotation2235984
version: 1
top level: pSB4K5
location: https://synbiohub.org/public/igem/pSB4K5/annotation2235984/range2235984/1
component: BBa_B0044

https://synbiohub.org/public/igem/pSB4K5/annotation2235984/range2235984/1
type: Range
persistent identity: range2235984
display id: range2235984
version: 1
direction: direction/reverse
top level: pSB4K5
start: 22
end: 34
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2235991
display id: annotation2235991
version: 1
top level: pSB4K5
location: https://synbiohub.org/public/igem/pSB4K5/annotation2235991/range2235991/1
component: BBa_B0042

https://synbiohub.org/public/igem/pSB4K5/annotation2235991/range2235991/1
type: Range
persistent identity: range2235991
display id: range2235991
version: 1
direction: direction/reverse
top level: pSB4K5
start: 35
end: 46
orientation: inline

BBa_P1007
type: SequenceAnnotation
persistent identity: annotation2236003
display id: annotation2236003
version: 1
top level: pSB4K5
location: https://synbiohub.org/public/igem/pSB4K5/annotation2236003/range2236003/1
component: BBa_P1007

https://synbiohub.org/public/igem/pSB4K5/annotation2236003/range2236003/1
type: Range
persistent identity: range2236003
display id: range2236003
version: 1
direction: direction/reverse
top level: pSB4K5
start: 184
end: 1150
orientation: inline

BBa_G00000
type: SequenceAnnotation
persistent identity: annotation2236055
display id: annotation2236055
version: 1
top level: pSB4K5
location: https://synbiohub.org/public/igem/pSB4K5/annotation2236055/range2236055/1
component: BBa_G00000

https://synbiohub.org/public/igem/pSB4K5/annotation2236055/range2236055/1
type: Range
persistent identity: range2236055
display id: range2236055
version: 1
direction: direction/reverse
top level: pSB4K5
start: 3398
end: 3419
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2236045
display id: annotation2236045
version: 1
top level: pSB4K5
location: https://synbiohub.org/public/igem/pSB4K5/annotation2236045/range2236045/1
component: BBa_B0042

https://synbiohub.org/public/igem/pSB4K5/annotation2236045/range2236045/1
type: Range
persistent identity: range2236045
display id: range2236045
version: 1
direction: direction/reverse
top level: pSB4K5
start: 3373
end: 3384
orientation: inline

BBa_G00100
type: SequenceAnnotation
persistent identity: annotation2236035
display id: annotation2236035
version: 1
top level: pSB4K5
location: https://synbiohub.org/public/igem/pSB4K5/annotation2236035/range2236035/1
component: BBa_G00100

https://synbiohub.org/public/igem/pSB4K5/annotation2236035/range2236035/1
type: Range
persistent identity: range2236035
display id: range2236035
version: 1
direction: direction/reverse
top level: pSB4K5
start: 3275
end: 3294
orientation: inline

BBa_G00001
type: SequenceAnnotation
persistent identity: annotation2235979
display id: annotation2235979
version: 1
top level: pSB4K5
location: https://synbiohub.org/public/igem/pSB4K5/annotation2235979/range2235979/1
component: BBa_G00001

https://synbiohub.org/public/igem/pSB4K5/annotation2235979/range2235979/1
type: Range
persistent identity: range2235979
display id: range2235979
version: 1
direction: direction/reverse
top level: pSB4K5
start: 1
end: 21
orientation: inline

BBa_B0053
type: SequenceAnnotation
persistent identity: annotation2236033
display id: annotation2236033
version: 1
top level: pSB4K5
location: https://synbiohub.org/public/igem/pSB4K5/annotation2236033/range2236033/1
component: BBa_B0053

https://synbiohub.org/public/igem/pSB4K5/annotation2236033/range2236033/1
type: Range
persistent identity: range2236033
display id: range2236033
version: 1
direction: direction/reverse
top level: pSB4K5
start: 3203
end: 3274
orientation: inline

BBa_G0001
type: SequenceAnnotation
persistent identity: annotation2236004
display id: annotation2236004
version: 1
top level: pSB4K5
location: https://synbiohub.org/public/igem/pSB4K5/annotation2236004/range2236004/1
component: BBa_G0001

https://synbiohub.org/public/igem/pSB4K5/annotation2236004/range2236004/1
type: Range
persistent identity: range2236004
display id: range2236004
version: 1
direction: direction/reverse
top level: pSB4K5
start: 1151
end: 1158
orientation: inline

BBa_G0006
type: SequenceAnnotation
persistent identity: annotation2236031
display id: annotation2236031
version: 1
top level: pSB4K5
location: https://synbiohub.org/public/igem/pSB4K5/annotation2236031/range2236031/1
component: BBa_G0006

https://synbiohub.org/public/igem/pSB4K5/annotation2236031/range2236031/1
type: Range
persistent identity: range2236031
display id: range2236031
version: 1
direction: direction/reverse
top level: pSB4K5
start: 3196
end: 3202
orientation: inline

BBa_G00102
type: SequenceAnnotation
persistent identity: annotation2235996
display id: annotation2235996
version: 1
top level: pSB4K5
location: https://synbiohub.org/public/igem/pSB4K5/annotation2235996/range2235996/1
component: BBa_G00102

https://synbiohub.org/public/igem/pSB4K5/annotation2235996/range2235996/1
type: Range
persistent identity: range2235996
display id: range2235996
version: 1
direction: direction/reverse
top level: pSB4K5
start: 116
end: 135
orientation: inline

BBa_I50041
type: SequenceAnnotation
persistent identity: annotation2236030
display id: annotation2236030
version: 1
top level: pSB4K5
location: https://synbiohub.org/public/igem/pSB4K5/annotation2236030/range2236030/1
component: BBa_I50041

https://synbiohub.org/public/igem/pSB4K5/annotation2236030/range2236030/1
type: Range
persistent identity: range2236030
display id: range2236030
version: 1
direction: direction/reverse
top level: pSB4K5
start: 1159
end: 3195
orientation: inline

BBa_B0062
type: SequenceAnnotation
persistent identity: annotation2235998
display id: annotation2235998
version: 1
top level: pSB4K5
location: https://synbiohub.org/public/igem/pSB4K5/annotation2235998/range2235998/1
component: BBa_B0062

https://synbiohub.org/public/igem/pSB4K5/annotation2235998/range2235998/1
type: Range
persistent identity: range2235998
display id: range2235998
version: 1
direction: direction/reverse
top level: pSB4K5
start: 136
end: 176
orientation: inline

BBa_B0043
type: SequenceAnnotation
persistent identity: annotation2236050
display id: annotation2236050
version: 1
top level: pSB4K5
location: https://synbiohub.org/public/igem/pSB4K5/annotation2236050/range2236050/1
component: BBa_B0043

https://synbiohub.org/public/igem/pSB4K5/annotation2236050/range2236050/1
type: Range
persistent identity: range2236050
display id: range2236050
version: 1
direction: direction/reverse
top level: pSB4K5
start: 3385
end: 3397
orientation: inline

BBa_B0055
type: SequenceAnnotation
persistent identity: annotation2236038
display id: annotation2236038
version: 1
top level: pSB4K5
location: https://synbiohub.org/public/igem/pSB4K5/annotation2236038/range2236038/1
component: BBa_B0055

https://synbiohub.org/public/igem/pSB4K5/annotation2236038/range2236038/1
type: Range
persistent identity: range2236038
display id: range2236038
version: 1
direction: direction/reverse
top level: pSB4K5
start: 3295
end: 3372
orientation: inline

BBa_K864003
type: ComponentDefinition
persistent identity: BBa K864003
display id: BBa_K864003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector
creator: Erik Lundin
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB4A15 is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and ampicillin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone. The pSC101 origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
mutable provenance: Based on pSB3T5
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: P22 late terminator
sequence annotation: rnC terminator
sequence annotation: VF2
sequence annotation: VR
sequence annotation: Lambda Red forward primer
sequence annotation: Promoter
sequence annotation: Lux terminator
sequence annotation: T3 terminator
sequence annotation: TonB terminator
sequence annotation: Lambda Red reverse primer
sequence annotation: spcR
sequence annotation: pSC101 ori BBa_K864050
sequence: https://synbiohub.org/public/igem/BBa_K864003_sequence/1

P22 late terminator
type: SequenceAnnotation
persistent identity: annotation2201243
display id: annotation2201243
version: 1
top level: BBa_K864003
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864003/annotation2201243/range2201243/1

https://synbiohub.org/public/igem/BBa_K864003/annotation2201243/range2201243/1
type: Range
persistent identity: range2201243
display id: range2201243
version: 1
direction: direction/forward
top level: BBa_K864003
start: 3608
end: 3648
orientation: inline

rnC terminator
type: SequenceAnnotation
persistent identity: annotation2201237
display id: annotation2201237
version: 1
top level: BBa_K864003
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864003/annotation2201237/range2201237/1

https://synbiohub.org/public/igem/BBa_K864003/annotation2201237/range2201237/1
type: Range
persistent identity: range2201237
display id: range2201237
version: 1
direction: direction/forward
top level: BBa_K864003
start: 136
end: 175
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation2201244
display id: annotation2201244
version: 1
top level: BBa_K864003
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864003/annotation2201244/range2201244/1

https://synbiohub.org/public/igem/BBa_K864003/annotation2201244/range2201244/1
type: Range
persistent identity: range2201244
display id: range2201244
version: 1
direction: direction/forward
top level: BBa_K864003
start: 3649
end: 3668
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation2201236
display id: annotation2201236
version: 1
top level: BBa_K864003
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864003/annotation2201236/range2201236/1

https://synbiohub.org/public/igem/BBa_K864003/annotation2201236/range2201236/1
type: Range
persistent identity: range2201236
display id: range2201236
version: 1
direction: direction/forward
top level: BBa_K864003
start: 116
end: 135
orientation: inline

Lambda Red forward primer
type: SequenceAnnotation
persistent identity: annotation2201239
display id: annotation2201239
version: 1
top level: BBa_K864003
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864003/annotation2201239/range2201239/1

https://synbiohub.org/public/igem/BBa_K864003/annotation2201239/range2201239/1
type: Range
persistent identity: range2201239
display id: range2201239
version: 1
direction: direction/forward
top level: BBa_K864003
start: 2433
end: 2450
orientation: inline

Promoter
type: SequenceAnnotation
persistent identity: annotation2201242
display id: annotation2201242
version: 1
top level: BBa_K864003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K864003/annotation2201242/range2201242/1

https://synbiohub.org/public/igem/BBa_K864003/annotation2201242/range2201242/1
type: Range
persistent identity: range2201242
display id: range2201242
version: 1
direction: direction/reverse
top level: BBa_K864003
start: 3575
end: 3601
orientation: inline

Lux terminator
type: SequenceAnnotation
persistent identity: annotation2201240
display id: annotation2201240
version: 1
top level: BBa_K864003
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864003/annotation2201240/range2201240/1

https://synbiohub.org/public/igem/BBa_K864003/annotation2201240/range2201240/1
type: Range
persistent identity: range2201240
display id: range2201240
version: 1
direction: direction/reverse
top level: BBa_K864003
start: 2454
end: 2499
orientation: inline

T3 terminator
type: SequenceAnnotation
persistent identity: annotation2201245
display id: annotation2201245
version: 1
top level: BBa_K864003
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864003/annotation2201245/range2201245/1

https://synbiohub.org/public/igem/BBa_K864003/annotation2201245/range2201245/1
type: Range
persistent identity: range2201245
display id: range2201245
version: 1
direction: direction/forward
top level: BBa_K864003
start: 3669
end: 3746
orientation: inline

TonB terminator
type: SequenceAnnotation
persistent identity: annotation2201235
display id: annotation2201235
version: 1
top level: BBa_K864003
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864003/annotation2201235/range2201235/1

https://synbiohub.org/public/igem/BBa_K864003/annotation2201235/range2201235/1
type: Range
persistent identity: range2201235
display id: range2201235
version: 1
direction: direction/forward
top level: BBa_K864003
start: 47
end: 115
orientation: inline

Lambda Red reverse primer
type: SequenceAnnotation
persistent identity: annotation2201234
display id: annotation2201234
version: 1
top level: BBa_K864003
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864003/annotation2201234/range2201234/1

https://synbiohub.org/public/igem/BBa_K864003/annotation2201234/range2201234/1
type: Range
persistent identity: range2201234
display id: range2201234
version: 1
direction: direction/reverse
top level: BBa_K864003
start: 18
end: 38
orientation: inline

spcR
type: SequenceAnnotation
persistent identity: annotation2201241
display id: annotation2201241
version: 1
top level: BBa_K864003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K864003/annotation2201241/range2201241/1

https://synbiohub.org/public/igem/BBa_K864003/annotation2201241/range2201241/1
type: Range
persistent identity: range2201241
display id: range2201241
version: 1
direction: direction/reverse
top level: BBa_K864003
start: 2510
end: 3508
orientation: inline

pSC101 ori BBa_K864050
type: SequenceAnnotation
persistent identity: annotation2201238
display id: annotation2201238
version: 1
top level: BBa_K864003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864003/annotation2201238/range2201238/1

https://synbiohub.org/public/igem/BBa_K864003/annotation2201238/range2201238/1
type: Range
persistent identity: range2201238
display id: range2201238
version: 1
direction: direction/forward
top level: BBa_K864003
start: 190
end: 2405
orientation: inline