//plasmidbackbone/proteinfusion
type: Collection
persistent identity: plasmidbackbone proteinfusion
display id: plasmidbackbone_proteinfusion
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: pMA-BBFR
member: pSB1AT3F
member: pSB1AK3F
member: pSB1AC3F
member: BBa_K249005
member: BBa_K249004
member: Silver vec
member: V0120

pSB1AT3F
type: ComponentDefinition
persistent identity: BBa J18903
display id: BBa_J18903
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB1AT3F construction plasmid for protein fusion BioBricks
creator: Raik Gruenberg
created: 2008-08-10T11:00:00Z
modified: 2015-08-31T04:08:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: pSB1AT3F is almost identical to pSB1AT3 but uses the "Freiburg" extension of the standard BioBrick prefix and suffix and features silent mutations in the tetracycline resistance gene. The Freiburg extension adds a new pair of inner restriction enzymes -- NgoMIV (prefix) and AgeI (suffix) -- which allows the in-frame fusion of protein parts. See [http://openwetware.org/wiki/The_BioBricks_Foundation:Standards/Technical/Formats BB formats]. pSB1AT3F is a high copy number plasmid carrying ampicillin and tetracyclin resistance. The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell). For the version of this plasmid with a CcdB insert, see BBa_P1010. The given sequence starts with the Freiburg BB suffix and ends with the Freiburg BB prefix. The complete vector sequence is therefore obtained from: part + vector
mutable notes: This plasmid was constructed by PCR and InFusion recombination. 1) The pSB1AK3 vector backbone was linearized by PCR, introducing the NgoMIV / AgeI restriction sites 2) The P1010 insert was amplified by PCR, introducing the NgoMIV / AgeI restriction sites 3) The two overlapping PCR products were recombined using the Clonetech InFusion kit. 4) Two NgoMIV restriction sites in the original pSB1AT3 (within the TcR) were deleted following the QuickChange protocol (from Qiagen). PCR reactions were performed with a proof-reading polymerase featuring a very low mutation rate. Nevertheless, the vector backbone may contain mutations with respect to pSB1AT3. Only insert and flanks have been verified by sequencing. The deletion of NgoMIV sites has been verified by restriction digests.
mutable provenance: constructed from pSB1AT3
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
sequence annotation: replication origin (MB1)
sequence annotation: extended BioBrick prefix
sequence annotation: extended BioBrick suffix
sequence annotation: BBVR
sequence annotation: BBVF2
sequence annotation: AmpR
sequence annotation: TetR
sequence: https://synbiohub.org/public/igem/BBa_J18903_sequence/1

replication origin (MB1)
type: SequenceAnnotation
persistent identity: annotation1971371
display id: annotation1971371
version: 1
top level: pSB1AT3F
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J18903/annotation1971371/range1971371/1

https://synbiohub.org/public/igem/BBa_J18903/annotation1971371/range1971371/1
type: Range
persistent identity: range1971371
display id: range1971371
version: 1
direction: direction/reverse
top level: pSB1AT3F
start: 270
end: 884
orientation: inline

extended BioBrick prefix
type: SequenceAnnotation
persistent identity: annotation1971366
display id: annotation1971366
version: 1
top level: pSB1AT3F
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J18903/annotation1971366/range1971366/1

https://synbiohub.org/public/igem/BBa_J18903/annotation1971366/range1971366/1
type: Range
persistent identity: range1971366
display id: range1971366
version: 1
direction: direction/forward
top level: pSB1AT3F
start: 3434
end: 3462
orientation: inline

extended BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation1971365
display id: annotation1971365
version: 1
top level: pSB1AT3F
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J18903/annotation1971365/range1971365/1

https://synbiohub.org/public/igem/BBa_J18903/annotation1971365/range1971365/1
type: Range
persistent identity: range1971365
display id: range1971365
version: 1
direction: direction/reverse
top level: pSB1AT3F
start: 1
end: 30
orientation: inline

BBVR
type: SequenceAnnotation
persistent identity: annotation1971367
display id: annotation1971367
version: 1
top level: pSB1AT3F
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J18903/annotation1971367/range1971367/1

https://synbiohub.org/public/igem/BBa_J18903/annotation1971367/range1971367/1
type: Range
persistent identity: range1971367
display id: range1971367
version: 1
direction: direction/reverse
top level: pSB1AT3F
start: 166
end: 185
orientation: inline

BBVF2
type: SequenceAnnotation
persistent identity: annotation1971368
display id: annotation1971368
version: 1
top level: pSB1AT3F
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J18903/annotation1971368/range1971368/1

https://synbiohub.org/public/igem/BBa_J18903/annotation1971368/range1971368/1
type: Range
persistent identity: range1971368
display id: range1971368
version: 1
direction: direction/forward
top level: pSB1AT3F
start: 3315
end: 3335
orientation: inline

AmpR
type: SequenceAnnotation
persistent identity: annotation1971369
display id: annotation1971369
version: 1
top level: pSB1AT3F
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J18903/annotation1971369/range1971369/1

https://synbiohub.org/public/igem/BBa_J18903/annotation1971369/range1971369/1
type: Range
persistent identity: range1971369
display id: range1971369
version: 1
direction: direction/reverse
top level: pSB1AT3F
start: 2333
end: 3193
orientation: inline

TetR
type: SequenceAnnotation
persistent identity: annotation1971370
display id: annotation1971370
version: 1
top level: pSB1AT3F
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J18903/annotation1971370/range1971370/1

https://synbiohub.org/public/igem/BBa_J18903/annotation1971370/range1971370/1
type: Range
persistent identity: range1971370
display id: range1971370
version: 1
direction: direction/forward
top level: pSB1AT3F
start: 1136
end: 2326
orientation: inline

BBa_K249005
type: ComponentDefinition
persistent identity: BBa K249005
display id: BBa_K249005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: C-terminal Arginine Fusion Vector
creator: Roxanne Shank
created: 2009-08-25T11:00:00Z
modified: 2015-05-08T01:11:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _342_
m user id: 0
owner id: 3171
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: pSB1A3 with an C-terminal Arginine tag. This particular plasmid utilizes the BioFusion (aka Silver Lab) Standard for BioBrick assembly. Adds 10 Arginine residues to the C-terminus of a Protein (Includes Stop Codon)
mutable notes: Standard
mutable provenance: Synthesized DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K249005_sequence/1

BBa_K249004
type: ComponentDefinition
persistent identity: BBa K249004
display id: BBa_K249004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: N-terminal Arginine fusion vector
creator: Roxanne Shank
created: 2009-08-25T11:00:00Z
modified: 2015-05-08T01:11:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _342_
m user id: 0
owner id: 3171
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: pSB1A3 with an N-terminal Arginine tag. This particular plasmid utilizes the BioFusion (aka Silver Lab) Standard for BioBrick assembly. Adds 10 Arginine residues to the N-terminus of a Protein.
mutable notes: Standard
mutable provenance: Synthesized Tag
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K249004_sequence/1

pSB1AC3F
type: ComponentDefinition
persistent identity: BBa J18901
display id: BBa_J18901
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB1AC3F construction plasmid for protein fusion BioBricks
creator: Raik Gruenberg
created: 2008-08-10T11:00:00Z
modified: 2015-08-31T04:08:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: pSB1AC3F is identical to pSB1AC3 but uses the "Freiburg" extension of the standard BioBrick prefix and suffix. This Freiburg extension adds a new pair of inner restriction enzymes -- NgoMIV (prefix) and AgeI (suffix) -- which allows the in-frame fusion of protein parts. See [http://openwetware.org/wiki/The_BioBricks_Foundation:Standards/Technical/Formats BB formats]. pSB1AC3F is a high copy number plasmid carrying ampicillin and chloramphenicol resistance. The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell). For the version of this plasmid with a CcdB insert, see BBa_P1010. The given sequence starts with the Freiburg BB suffix and ends with the Freiburg BB prefix. The complete vector sequence is therefore obtained from: part + vector
mutable notes: This plasmid was constructed by PCR and InFusion recombination. 1) The pSB1AC3 vector backbone was linearized by PCR, introducing the NgoMIV / AgeI restriction sites 2) The P1010 insert was amplified by PCR, introducing the NgoMIV / AgeI restriction sites 3) The two overlapping PCR products were recombined using the clonetech InFusion kit. All PCR reactions were performed with a proof-reading polymerase featuring a very low mutation rate. Nevertheless, the vector backbone may contain mutations with respect to pSB1AC3. Only insert and flanks have been verified by sequencing.
mutable provenance: constructed from pSB1AC3
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
sequence annotation: extended BioBrick prefix
sequence annotation: BBVF2
sequence annotation: BBVR (G00101)
sequence annotation: extended BioBrick suffix
sequence annotation: ChlR
sequence annotation: Replication Origin (MB1)
sequence annotation: AmpR
sequence: https://synbiohub.org/public/igem/BBa_J18901_sequence/1

extended BioBrick prefix
type: SequenceAnnotation
persistent identity: annotation1971352
display id: annotation1971352
version: 1
top level: pSB1AC3F
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J18901/annotation1971352/range1971352/1

https://synbiohub.org/public/igem/BBa_J18901/annotation1971352/range1971352/1
type: Range
persistent identity: range1971352
display id: range1971352
version: 1
direction: direction/forward
top level: pSB1AC3F
start: 3043
end: 3071
orientation: inline

BBVF2
type: SequenceAnnotation
persistent identity: annotation1971353
display id: annotation1971353
version: 1
top level: pSB1AC3F
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J18901/annotation1971353/range1971353/1

https://synbiohub.org/public/igem/BBa_J18901/annotation1971353/range1971353/1
type: Range
persistent identity: range1971353
display id: range1971353
version: 1
direction: direction/forward
top level: pSB1AC3F
start: 2924
end: 2944
orientation: inline

BBVR (G00101)
type: SequenceAnnotation
persistent identity: annotation1971354
display id: annotation1971354
version: 1
top level: pSB1AC3F
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J18901/annotation1971354/range1971354/1

https://synbiohub.org/public/igem/BBa_J18901/annotation1971354/range1971354/1
type: Range
persistent identity: range1971354
display id: range1971354
version: 1
direction: direction/reverse
top level: pSB1AC3F
start: 166
end: 185
orientation: inline

extended BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation1971351
display id: annotation1971351
version: 1
top level: pSB1AC3F
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J18901/annotation1971351/range1971351/1

https://synbiohub.org/public/igem/BBa_J18901/annotation1971351/range1971351/1
type: Range
persistent identity: range1971351
display id: range1971351
version: 1
direction: direction/reverse
top level: pSB1AC3F
start: 1
end: 30
orientation: inline

ChlR
type: SequenceAnnotation
persistent identity: annotation1971356
display id: annotation1971356
version: 1
top level: pSB1AC3F
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J18901/annotation1971356/range1971356/1

https://synbiohub.org/public/igem/BBa_J18901/annotation1971356/range1971356/1
type: Range
persistent identity: range1971356
display id: range1971356
version: 1
direction: direction/reverse
top level: pSB1AC3F
start: 1173
end: 1830
orientation: inline

Replication Origin (MB1)
type: SequenceAnnotation
persistent identity: annotation1971357
display id: annotation1971357
version: 1
top level: pSB1AC3F
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J18901/annotation1971357/range1971357/1

https://synbiohub.org/public/igem/BBa_J18901/annotation1971357/range1971357/1
type: Range
persistent identity: range1971357
display id: range1971357
version: 1
direction: direction/reverse
top level: pSB1AC3F
start: 270
end: 883
orientation: inline

AmpR
type: SequenceAnnotation
persistent identity: annotation1971355
display id: annotation1971355
version: 1
top level: pSB1AC3F
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J18901/annotation1971355/range1971355/1

https://synbiohub.org/public/igem/BBa_J18901/annotation1971355/range1971355/1
type: Range
persistent identity: range1971355
display id: range1971355
version: 1
direction: direction/reverse
top level: pSB1AC3F
start: 1942
end: 2802
orientation: inline

pSB1AK3F
type: ComponentDefinition
persistent identity: BBa J18902
display id: BBa_J18902
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB1AK3F construction plasmid for protein fusion BioBricks
creator: Raik Gruenberg
created: 2008-08-10T11:00:00Z
modified: 2015-08-31T04:08:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: pSB1AK3F is identical to pSB1AK3 but uses the "Freiburg" extension of the standard BioBrick prefix and suffix. This Freiburg extension adds a new pair of inner restriction enzymes -- NgoMIV (prefix) and AgeI (suffix) -- which allows the in-frame fusion of protein parts. See [http://openwetware.org/wiki/The_BioBricks_Foundation:Standards/Technical/Formats BB formats]. pSB1AK3F is a high copy number plasmid carrying ampicillin and kanamycin resistance. The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell). For the version of this plasmid with a CcdB insert, see BBa_P1010. The given sequence starts with the Freiburg BB suffix and ends with the Freiburg BB prefix. The complete vector sequence is therefore obtained from: part + vector
mutable notes: This plasmid was constructed by PCR and InFusion recombination. 1) The pSB1AK3 vector backbone was linearized by PCR, introducing the NgoMIV / AgeI restriction sites 2) The P1010 insert was amplified by PCR, introducing the NgoMIV / AgeI restriction sites 3) The two overlapping PCR products were recombined using the clonetech InFusion kit. All PCR reactions were performed with a proof-reading polymerase featuring a very low mutation rate. Nevertheless, the vector backbone may contain mutations with respect to pSB1AC3. Only insert and flanks have been verified by sequencing.
mutable provenance: constructed from pSB1AK3
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
sequence annotation: extended BioBrick prefix
sequence annotation: Replication Origin (MB1)
sequence annotation: AmpR
sequence annotation: KanR
sequence annotation: BBVR
sequence annotation: BBF2
sequence annotation: extended BioBrick suffix
sequence: https://synbiohub.org/public/igem/BBa_J18902_sequence/1

extended BioBrick prefix
type: SequenceAnnotation
persistent identity: annotation1971359
display id: annotation1971359
version: 1
top level: pSB1AK3F
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J18902/annotation1971359/range1971359/1

https://synbiohub.org/public/igem/BBa_J18902/annotation1971359/range1971359/1
type: Range
persistent identity: range1971359
display id: range1971359
version: 1
direction: direction/forward
top level: pSB1AK3F
start: 3177
end: 3205
orientation: inline

Replication Origin (MB1)
type: SequenceAnnotation
persistent identity: annotation1971364
display id: annotation1971364
version: 1
top level: pSB1AK3F
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J18902/annotation1971364/range1971364/1

https://synbiohub.org/public/igem/BBa_J18902/annotation1971364/range1971364/1
type: Range
persistent identity: range1971364
display id: range1971364
version: 1
direction: direction/reverse
top level: pSB1AK3F
start: 270
end: 883
orientation: inline

AmpR
type: SequenceAnnotation
persistent identity: annotation1971361
display id: annotation1971361
version: 1
top level: pSB1AK3F
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J18902/annotation1971361/range1971361/1

https://synbiohub.org/public/igem/BBa_J18902/annotation1971361/range1971361/1
type: Range
persistent identity: range1971361
display id: range1971361
version: 1
direction: direction/forward
top level: pSB1AK3F
start: 2076
end: 2936
orientation: inline

KanR
type: SequenceAnnotation
persistent identity: annotation1971360
display id: annotation1971360
version: 1
top level: pSB1AK3F
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J18902/annotation1971360/range1971360/1

https://synbiohub.org/public/igem/BBa_J18902/annotation1971360/range1971360/1
type: Range
persistent identity: range1971360
display id: range1971360
version: 1
direction: direction/reverse
top level: pSB1AK3F
start: 1106
end: 1921
orientation: inline

BBVR
type: SequenceAnnotation
persistent identity: annotation1971363
display id: annotation1971363
version: 1
top level: pSB1AK3F
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J18902/annotation1971363/range1971363/1

https://synbiohub.org/public/igem/BBa_J18902/annotation1971363/range1971363/1
type: Range
persistent identity: range1971363
display id: range1971363
version: 1
direction: direction/reverse
top level: pSB1AK3F
start: 166
end: 185
orientation: inline

BBF2
type: SequenceAnnotation
persistent identity: annotation1971362
display id: annotation1971362
version: 1
top level: pSB1AK3F
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J18902/annotation1971362/range1971362/1

https://synbiohub.org/public/igem/BBa_J18902/annotation1971362/range1971362/1
type: Range
persistent identity: range1971362
display id: range1971362
version: 1
direction: direction/forward
top level: pSB1AK3F
start: 3058
end: 3078
orientation: inline

extended BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation1971358
display id: annotation1971358
version: 1
top level: pSB1AK3F
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J18902/annotation1971358/range1971358/1

https://synbiohub.org/public/igem/BBa_J18902/annotation1971358/range1971358/1
type: Range
persistent identity: range1971358
display id: range1971358
version: 1
direction: direction/reverse
top level: pSB1AK3F
start: 1
end: 30
orientation: inline

Silver vec
type: ComponentDefinition
persistent identity: BBa J63009
display id: BBa_J63009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy protein fusion vector (Silver lab standard)
creator: Caroline Ajo-Franklin
created: 2006-10-13T11:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _97_
m user id: 0
owner id: 545
owning group id: 97
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Silver Lab alias V0002. This is a modified biobrick vector (fusion brick vector) that contains the specified EcoRI, SpeI, XbaI, and PstI sites. The only difference is that the part sequence immediately follows the XbaI site and immediately precedes the SpeI site. This change allows in-frame fusions of protein coding regions to be made using standard biobricks assembly methods.
mutable notes: If the part starts with the nucleotides tc, then that part-containing plasmid must be grow in a DamI- strain to avoid methylation (and inhibition of digestion) at the XbaI site.
mutable provenance: based on pUC19 plasmid
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: rep pMB1 replication origin
sequence annotation: bla(P3) promoter -10 sequence
sequence annotation: RNAII transcript
sequence annotation: beta-lactamase (bla gene)
sequence annotation: bla gene
sequence annotation: RNAI transcript
sequence annotation: bla(P3) promoter -35 sequence
sequence annotation: modified BioBrick prefix
sequence annotation: modified BioBrick suffix
sequence: https://synbiohub.org/public/igem/BBa_J63009_sequence/1

rep pMB1 replication origin
type: SequenceAnnotation
persistent identity: annotation1969661
display id: annotation1969661
version: 1
top level: Silver vec
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J63009/annotation1969661/range1969661/1

https://synbiohub.org/public/igem/BBa_J63009/annotation1969661/range1969661/1
type: Range
persistent identity: range1969661
display id: range1969661
version: 1
direction: direction/reverse
top level: Silver vec
start: 182
end: 796
orientation: inline

bla(P3) promoter -10 sequence
type: SequenceAnnotation
persistent identity: annotation1969665
display id: annotation1969665
version: 1
top level: Silver vec
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J63009/annotation1969665/range1969665/1

https://synbiohub.org/public/igem/BBa_J63009/annotation1969665/range1969665/1
type: Range
persistent identity: range1969665
display id: range1969665
version: 1
direction: direction/reverse
top level: Silver vec
start: 1860
end: 1865
orientation: inline

RNAII transcript
type: SequenceAnnotation
persistent identity: annotation1969660
display id: annotation1969660
version: 1
top level: Silver vec
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J63009/annotation1969660/range1969660/1

https://synbiohub.org/public/igem/BBa_J63009/annotation1969660/range1969660/1
type: Range
persistent identity: range1969660
display id: range1969660
version: 1
direction: direction/reverse
top level: Silver vec
start: 197
end: 749
orientation: inline

beta-lactamase (bla gene)
type: SequenceAnnotation
persistent identity: annotation1969663
display id: annotation1969663
version: 1
top level: Silver vec
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J63009/annotation1969663/range1969663/1

https://synbiohub.org/public/igem/BBa_J63009/annotation1969663/range1969663/1
type: Range
persistent identity: range1969663
display id: range1969663
version: 1
direction: direction/reverse
top level: Silver vec
start: 956
end: 1816
orientation: inline

bla gene
type: SequenceAnnotation
persistent identity: annotation1969664
display id: annotation1969664
version: 1
top level: Silver vec
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J63009/annotation1969664/range1969664/1

https://synbiohub.org/public/igem/BBa_J63009/annotation1969664/range1969664/1
type: Range
persistent identity: range1969664
display id: range1969664
version: 1
direction: direction/reverse
top level: Silver vec
start: 1824
end: 1828
orientation: inline

RNAI transcript
type: SequenceAnnotation
persistent identity: annotation1969662
display id: annotation1969662
version: 1
top level: Silver vec
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J63009/annotation1969662/range1969662/1

https://synbiohub.org/public/igem/BBa_J63009/annotation1969662/range1969662/1
type: Range
persistent identity: range1969662
display id: range1969662
version: 1
direction: direction/forward
top level: Silver vec
start: 639
end: 746
orientation: inline

bla(P3) promoter -35 sequence
type: SequenceAnnotation
persistent identity: annotation1969666
display id: annotation1969666
version: 1
top level: Silver vec
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J63009/annotation1969666/range1969666/1

https://synbiohub.org/public/igem/BBa_J63009/annotation1969666/range1969666/1
type: Range
persistent identity: range1969666
display id: range1969666
version: 1
direction: direction/reverse
top level: Silver vec
start: 1881
end: 1886
orientation: inline

modified BioBrick prefix
type: SequenceAnnotation
persistent identity: annotation1969667
display id: annotation1969667
version: 1
top level: Silver vec
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J63009/annotation1969667/range1969667/1

https://synbiohub.org/public/igem/BBa_J63009/annotation1969667/range1969667/1
type: Range
persistent identity: range1969667
display id: range1969667
version: 1
direction: direction/forward
top level: Silver vec
start: 2057
end: 2077
orientation: inline

modified BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation1969659
display id: annotation1969659
version: 1
top level: Silver vec
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J63009/annotation1969659/range1969659/1

https://synbiohub.org/public/igem/BBa_J63009/annotation1969659/range1969659/1
type: Range
persistent identity: range1969659
display id: range1969659
version: 1
direction: direction/forward
top level: Silver vec
start: 1
end: 20
orientation: inline

pMA-BBFR
type: ComponentDefinition
persistent identity: BBa K157000
display id: BBa_K157000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pMA-BBFR - plasmid description
creator: Raik Gruenberg and iGEM Team Freiburg
created: 2008-10-23T11:00:00Z
modified: 2015-05-08T01:10:54Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _232_
m user id: 0
owner id: 1675
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Plasmid with BioBrick 3.0 prefix and suffix
mutable notes: none
mutable provenance: Plasmid designed by Raik Groenberg
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: NgoMIV
sequence annotation: Prefix BioBrick 3.0
sequence annotation: AgeI
sequence annotation: stop
sequence annotation: start
sequence annotation: Suffix BioBrick 3.0
sequence annotation: AmpR
sequence: https://synbiohub.org/public/igem/BBa_K157000_sequence/1

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2064660
display id: annotation2064660
version: 1
top level: pMA-BBFR
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157000/annotation2064660/range2064660/1

https://synbiohub.org/public/igem/BBa_K157000/annotation2064660/range2064660/1
type: Range
persistent identity: range2064660
display id: range2064660
version: 1
direction: direction/forward
top level: pMA-BBFR
start: 2423
end: 2428
orientation: inline

Prefix BioBrick 3.0
type: SequenceAnnotation
persistent identity: annotation1986395
display id: annotation1986395
version: 1
top level: pMA-BBFR
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157000/annotation1986395/range1986395/1

https://synbiohub.org/public/igem/BBa_K157000/annotation1986395/range1986395/1
type: Range
persistent identity: range1986395
display id: range1986395
version: 1
direction: direction/forward
top level: pMA-BBFR
start: 2400
end: 2428
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation1986391
display id: annotation1986391
version: 1
top level: pMA-BBFR
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157000/annotation1986391/range1986391/1

https://synbiohub.org/public/igem/BBa_K157000/annotation1986391/range1986391/1
type: Range
persistent identity: range1986391
display id: range1986391
version: 1
direction: direction/forward
top level: pMA-BBFR
start: 1
end: 6
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1986393
display id: annotation1986393
version: 1
top level: pMA-BBFR
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K157000/annotation1986393/range1986393/1

https://synbiohub.org/public/igem/BBa_K157000/annotation1986393/range1986393/1
type: Range
persistent identity: range1986393
display id: range1986393
version: 1
direction: direction/forward
top level: pMA-BBFR
start: 7
end: 9
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1986396
display id: annotation1986396
version: 1
top level: pMA-BBFR
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K157000/annotation1986396/range1986396/1

https://synbiohub.org/public/igem/BBa_K157000/annotation1986396/range1986396/1
type: Range
persistent identity: range1986396
display id: range1986396
version: 1
direction: direction/forward
top level: pMA-BBFR
start: 2420
end: 2422
orientation: inline

Suffix BioBrick 3.0
type: SequenceAnnotation
persistent identity: annotation1986392
display id: annotation1986392
version: 1
top level: pMA-BBFR
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157000/annotation1986392/range1986392/1

https://synbiohub.org/public/igem/BBa_K157000/annotation1986392/range1986392/1
type: Range
persistent identity: range1986392
display id: range1986392
version: 1
direction: direction/forward
top level: pMA-BBFR
start: 1
end: 30
orientation: inline

AmpR
type: SequenceAnnotation
persistent identity: annotation1986394
display id: annotation1986394
version: 1
top level: pMA-BBFR
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157000/annotation1986394/range1986394/1

https://synbiohub.org/public/igem/BBa_K157000/annotation1986394/range1986394/1
type: Range
persistent identity: range1986394
display id: range1986394
version: 1
direction: direction/forward
top level: pMA-BBFR
start: 541
end: 1398
orientation: inline

V0120
type: ComponentDefinition
persistent identity: BBa J63010
display id: BBa_J63010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Protein fusion vector (Silver lab standard)
creator: Ira Phillips and Caroline Ajo-Franklin
created: 2006-10-17T11:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _97_
m user id: 0
owner id: 545
owning group id: 97
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: standard biofusion plasmid containing ccdB death gene and chlorenphenicol resistance
mutable notes: transform into TOP10 survival
mutable provenance: synthetized DNA
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: f1 origin
sequence annotation: beta-lactamase (bla gene)
sequence annotation: RNAI transcript
sequence annotation: modified BioBrick suffix
sequence annotation: modified BioBrick prefix
sequence annotation: incorrect silver fusion prefix
sequence annotation: RNAII transcript
sequence annotation: lacZ alpha peptide
sequence annotation: rep pMB1 replication origin
sequence: https://synbiohub.org/public/igem/BBa_J63010_sequence/1

f1 origin
type: SequenceAnnotation
persistent identity: annotation1969668
display id: annotation1969668
version: 1
top level: V0120
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J63010/annotation1969668/range1969668/1

https://synbiohub.org/public/igem/BBa_J63010/annotation1969668/range1969668/1
type: Range
persistent identity: range1969668
display id: range1969668
version: 1
direction: direction/forward
top level: V0120
start: 2632
end: 2938
orientation: inline

beta-lactamase (bla gene)
type: SequenceAnnotation
persistent identity: annotation1908165
display id: annotation1908165
version: 1
top level: V0120
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J63010/annotation1908165/range1908165/1

https://synbiohub.org/public/igem/BBa_J63010/annotation1908165/range1908165/1
type: Range
persistent identity: range1908165
display id: range1908165
version: 1
direction: direction/reverse
top level: V0120
start: 1253
end: 2113
orientation: inline

RNAI transcript
type: SequenceAnnotation
persistent identity: annotation1969671
display id: annotation1969671
version: 1
top level: V0120
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J63010/annotation1969671/range1969671/1

https://synbiohub.org/public/igem/BBa_J63010/annotation1969671/range1969671/1
type: Range
persistent identity: range1969671
display id: range1969671
version: 1
direction: direction/forward
top level: V0120
start: 936
end: 1043
orientation: inline

modified BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation1908166
display id: annotation1908166
version: 1
top level: V0120
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J63010/annotation1908166/range1908166/1

https://synbiohub.org/public/igem/BBa_J63010/annotation1908166/range1908166/1
type: Range
persistent identity: range1908166
display id: range1908166
version: 1
direction: direction/forward
top level: V0120
start: 1
end: 20
orientation: inline

modified BioBrick prefix
type: SequenceAnnotation
persistent identity: annotation1969672
display id: annotation1969672
version: 1
top level: V0120
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J63010/annotation1969672/range1969672/1

https://synbiohub.org/public/igem/BBa_J63010/annotation1969672/range1969672/1
type: Range
persistent identity: range1969672
display id: range1969672
version: 1
direction: direction/forward
top level: V0120
start: 3246
end: 3266
orientation: inline

incorrect silver fusion prefix
type: SequenceAnnotation
persistent identity: annotation2172657
display id: annotation2172657
version: 1
top level: V0120
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J63010/annotation2172657/range2172657/1

https://synbiohub.org/public/igem/BBa_J63010/annotation2172657/range2172657/1
type: Range
persistent identity: range2172657
display id: range2172657
version: 1
direction: direction/forward
top level: V0120
start: 3260
end: 3260
orientation: inline

RNAII transcript
type: SequenceAnnotation
persistent identity: annotation1969670
display id: annotation1969670
version: 1
top level: V0120
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J63010/annotation1969670/range1969670/1

https://synbiohub.org/public/igem/BBa_J63010/annotation1969670/range1969670/1
type: Range
persistent identity: range1969670
display id: range1969670
version: 1
direction: direction/reverse
top level: V0120
start: 494
end: 1046
orientation: inline

lacZ alpha peptide
type: SequenceAnnotation
persistent identity: annotation1969669
display id: annotation1969669
version: 1
top level: V0120
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J63010/annotation1969669/range1969669/1

https://synbiohub.org/public/igem/BBa_J63010/annotation1969669/range1969669/1
type: Range
persistent identity: range1969669
display id: range1969669
version: 1
direction: direction/reverse
top level: V0120
start: 3028
end: 3187
orientation: inline

rep pMB1 replication origin
type: SequenceAnnotation
persistent identity: annotation1908167
display id: annotation1908167
version: 1
top level: V0120
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J63010/annotation1908167/range1908167/1

https://synbiohub.org/public/igem/BBa_J63010/annotation1908167/range1908167/1
type: Range
persistent identity: range1908167
display id: range1908167
version: 1
direction: direction/forward
top level: V0120
start: 479
end: 1093
orientation: inline