//plasmidbackbone/libraryscreening/rbscodingsequence
type: Collection
persistent identity: plasmidbackbone libraryscreening rbscodingsequence
display id: plasmidbackbone_libraryscreening_rbscodingsequence
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_I732950
member: BBa_I732952
member: BBa_J23006

BBa_I732952
type: ComponentDefinition
persistent identity: BBa I732952
display id: BBa_I732952
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ptemplate1 + RBS.ARL2A0103
creator: Zhan Jian
created: 2007-10-25T11:00:00Z
modified: 2015-08-31T04:08:00Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: {| border="1" |- |align="center"|'''Genotype''' |align="center"|'''Character''' |align="center"|'''Name''' |- |Top10/pT-TERM |So not express any repressors |align="center"|CR00 |- |Top10/pT-ARL4A0604 |Constitutively express ARL4A0604 |align="center"|CR01 |- |Top10/pT-ARL2A0203 |Constitutively express ARL2A0203 |align="center"|CR10 |- |Top10/pTet-ARL4A0604-ARL2A203 |Constitutively express ARL4A0604 and ARL2A0203 |align="center"|CR11 |}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: pBR322 origin
sequence annotation: Stem loop
sequence annotation: beta-lactamase (ampR) promoter
sequence annotation: beta-lactamase (ampicillin resistance marker)
sequence annotation: EcoRI
sequence annotation: Verification forward (VF2) primer binding site
sequence annotation: BioBrick suffix
sequence annotation: E. coli his operon terminator
sequence annotation: Ptemplate1?
sequence annotation: End of terminator
sequence annotation: Verification reverse (VR) primer binding site
sequence annotation: Deletion of AC
sequence annotation: XbaI
sequence: https://synbiohub.org/public/igem/BBa_I732952_sequence/1

pBR322 origin
type: SequenceAnnotation
persistent identity: annotation1975785
display id: annotation1975785
version: 1
top level: BBa_I732952
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_I732952/annotation1975785/range1975785/1

https://synbiohub.org/public/igem/BBa_I732952/annotation1975785/range1975785/1
type: Range
persistent identity: range1975785
display id: range1975785
version: 1
direction: direction/reverse
top level: BBa_I732952
start: 261
end: 880
orientation: inline

Stem loop
type: SequenceAnnotation
persistent identity: annotation1975795
display id: annotation1975795
version: 1
top level: BBa_I732952
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I732952/annotation1975795/range1975795/1

https://synbiohub.org/public/igem/BBa_I732952/annotation1975795/range1975795/1
type: Range
persistent identity: range1975795
display id: range1975795
version: 1
direction: direction/forward
top level: BBa_I732952
start: 30
end: 64
orientation: inline

beta-lactamase (ampR) promoter
type: SequenceAnnotation
persistent identity: annotation1975787
display id: annotation1975787
version: 1
top level: BBa_I732952
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I732952/annotation1975787/range1975787/1

https://synbiohub.org/public/igem/BBa_I732952/annotation1975787/range1975787/1
type: Range
persistent identity: range1975787
display id: range1975787
version: 1
direction: direction/reverse
top level: BBa_I732952
start: 1937
end: 1965
orientation: inline

beta-lactamase (ampicillin resistance marker)
type: SequenceAnnotation
persistent identity: annotation1975786
display id: annotation1975786
version: 1
top level: BBa_I732952
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I732952/annotation1975786/range1975786/1

https://synbiohub.org/public/igem/BBa_I732952/annotation1975786/range1975786/1
type: Range
persistent identity: range1975786
display id: range1975786
version: 1
direction: direction/reverse
top level: BBa_I732952
start: 1035
end: 1895
orientation: inline

EcoRI
type: SequenceAnnotation
persistent identity: annotation1975788
display id: annotation1975788
version: 1
top level: BBa_I732952
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_I732952/annotation1975788/range1975788/1

https://synbiohub.org/public/igem/BBa_I732952/annotation1975788/range1975788/1
type: Range
persistent identity: range1975788
display id: range1975788
version: 1
direction: direction/forward
top level: BBa_I732952
start: 2136
end: 2141
orientation: inline

Verification forward (VF2) primer binding site
type: SequenceAnnotation
persistent identity: annotation1975791
display id: annotation1975791
version: 1
top level: BBa_I732952
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_I732952/annotation1975791/range1975791/1

https://synbiohub.org/public/igem/BBa_I732952/annotation1975791/range1975791/1
type: Range
persistent identity: range1975791
display id: range1975791
version: 1
direction: direction/forward
top level: BBa_I732952
start: 2018
end: 2037
orientation: inline

BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation1975789
display id: annotation1975789
version: 1
top level: BBa_I732952
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_I732952/annotation1975789/range1975789/1

https://synbiohub.org/public/igem/BBa_I732952/annotation1975789/range1975789/1
type: Range
persistent identity: range1975789
display id: range1975789
version: 1
direction: direction/forward
top level: BBa_I732952
start: 1
end: 21
orientation: inline

E. coli his operon terminator
type: SequenceAnnotation
persistent identity: annotation1975794
display id: annotation1975794
version: 1
top level: BBa_I732952
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I732952/annotation1975794/range1975794/1

https://synbiohub.org/public/igem/BBa_I732952/annotation1975794/range1975794/1
type: Range
persistent identity: range1975794
display id: range1975794
version: 1
direction: direction/forward
top level: BBa_I732952
start: 22
end: 93
orientation: inline

Stem loop
type: SequenceAnnotation
persistent identity: annotation1975792
display id: annotation1975792
version: 1
top level: BBa_I732952
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I732952/annotation1975792/range1975792/1

https://synbiohub.org/public/igem/BBa_I732952/annotation1975792/range1975792/1
type: Range
persistent identity: range1975792
display id: range1975792
version: 1
direction: direction/reverse
top level: BBa_I732952
start: 2107
end: 2126
orientation: inline

Ptemplate1?
type: SequenceAnnotation
persistent identity: annotation1975798
display id: annotation1975798
version: 1
top level: BBa_I732952
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I732952/annotation1975798/range1975798/1

https://synbiohub.org/public/igem/BBa_I732952/annotation1975798/range1975798/1
type: Range
persistent identity: range1975798
display id: range1975798
version: 1
direction: direction/forward
top level: BBa_I732952
start: 2142
end: 2300
orientation: inline

End of terminator
type: SequenceAnnotation
persistent identity: annotation1975793
display id: annotation1975793
version: 1
top level: BBa_I732952
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I732952/annotation1975793/range1975793/1

https://synbiohub.org/public/igem/BBa_I732952/annotation1975793/range1975793/1
type: Range
persistent identity: range1975793
display id: range1975793
version: 1
direction: direction/reverse
top level: BBa_I732952
start: 2094
end: 2097
orientation: inline

Verification reverse (VR) primer binding site
type: SequenceAnnotation
persistent identity: annotation1975797
display id: annotation1975797
version: 1
top level: BBa_I732952
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_I732952/annotation1975797/range1975797/1

https://synbiohub.org/public/igem/BBa_I732952/annotation1975797/range1975797/1
type: Range
persistent identity: range1975797
display id: range1975797
version: 1
direction: direction/reverse
top level: BBa_I732952
start: 157
end: 176
orientation: inline

Deletion of AC
type: SequenceAnnotation
persistent identity: annotation1975796
display id: annotation1975796
version: 1
top level: BBa_I732952
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_I732952/annotation1975796/range1975796/1

https://synbiohub.org/public/igem/BBa_I732952/annotation1975796/range1975796/1
type: Range
persistent identity: range1975796
display id: range1975796
version: 1
direction: direction/forward
top level: BBa_I732952
start: 34
end: 35
orientation: inline

XbaI
type: SequenceAnnotation
persistent identity: annotation1975790
display id: annotation1975790
version: 1
top level: BBa_I732952
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_I732952/annotation1975790/range1975790/1

https://synbiohub.org/public/igem/BBa_I732952/annotation1975790/range1975790/1
type: Range
persistent identity: range1975790
display id: range1975790
version: 1
direction: direction/forward
top level: BBa_I732952
start: 2301
end: 2306
orientation: inline

BBa_J23006
type: ComponentDefinition
persistent identity: BBa J23006
display id: BBa_J23006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Part-making plasmid with Ptet-XbaI-[biobrick]-SpeI-PstI
creator: John Anderson
created: 2006-06-30T11:00:00Z
modified: 2015-08-31T04:08:38Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: Plasmid for constructing biobrick parts that provides a minimal set of functions to observe activity.
mutable notes: N/A
mutable provenance: pSB1A2-I13521 See KAD062806-Construction of J23006 for details
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: EcoRI
sequence annotation: XbaI
sequence annotation: beta-lactamase ampicillin resistance marker
sequence annotation: BBa_B0015 terminator
sequence annotation: R0040 (pTet promoter)
sequence annotation: BioBrick suffix
sequence annotation: ColE1 origin
sequence annotation: rrnB T1 terminator
sequence annotation: pBR322 origin
sequence annotation: NotI
sequence annotation: beta-lactamase promoter
sequence: https://synbiohub.org/public/igem/BBa_J23006_sequence/1

EcoRI
type: SequenceAnnotation
persistent identity: annotation1975728
display id: annotation1975728
version: 1
top level: BBa_J23006
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J23006/annotation1975728/range1975728/1

https://synbiohub.org/public/igem/BBa_J23006/annotation1975728/range1975728/1
type: Range
persistent identity: range1975728
display id: range1975728
version: 1
direction: direction/forward
top level: BBa_J23006
start: 2136
end: 2141
orientation: inline

XbaI
type: SequenceAnnotation
persistent identity: annotation1975730
display id: annotation1975730
version: 1
top level: BBa_J23006
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J23006/annotation1975730/range1975730/1

https://synbiohub.org/public/igem/BBa_J23006/annotation1975730/range1975730/1
type: Range
persistent identity: range1975730
display id: range1975730
version: 1
direction: direction/forward
top level: BBa_J23006
start: 2350
end: 2355
orientation: inline

beta-lactamase ampicillin resistance marker
type: SequenceAnnotation
persistent identity: annotation1975723
display id: annotation1975723
version: 1
top level: BBa_J23006
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J23006/annotation1975723/range1975723/1

https://synbiohub.org/public/igem/BBa_J23006/annotation1975723/range1975723/1
type: Range
persistent identity: range1975723
display id: range1975723
version: 1
direction: direction/reverse
top level: BBa_J23006
start: 1035
end: 1895
orientation: inline

BBa_B0015 terminator
type: SequenceAnnotation
persistent identity: annotation1975722
display id: annotation1975722
version: 1
top level: BBa_J23006
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J23006/annotation1975722/range1975722/1

https://synbiohub.org/public/igem/BBa_J23006/annotation1975722/range1975722/1
type: Range
persistent identity: range1975722
display id: range1975722
version: 1
direction: direction/forward
top level: BBa_J23006
start: 2158
end: 2286
orientation: inline

R0040 (pTet promoter)
type: SequenceAnnotation
persistent identity: annotation1975720
display id: annotation1975720
version: 1
top level: BBa_J23006
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J23006/annotation1975720/range1975720/1

https://synbiohub.org/public/igem/BBa_J23006/annotation1975720/range1975720/1
type: Range
persistent identity: range1975720
display id: range1975720
version: 1
direction: direction/forward
top level: BBa_J23006
start: 2295
end: 2348
orientation: inline

BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation1975727
display id: annotation1975727
version: 1
top level: BBa_J23006
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J23006/annotation1975727/range1975727/1

https://synbiohub.org/public/igem/BBa_J23006/annotation1975727/range1975727/1
type: Range
persistent identity: range1975727
display id: range1975727
version: 1
direction: direction/forward
top level: BBa_J23006
start: 1
end: 21
orientation: inline

ColE1 origin
type: SequenceAnnotation
persistent identity: annotation1975721
display id: annotation1975721
version: 1
top level: BBa_J23006
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J23006/annotation1975721/range1975721/1

https://synbiohub.org/public/igem/BBa_J23006/annotation1975721/range1975721/1
type: Range
persistent identity: range1975721
display id: range1975721
version: 1
direction: direction/reverse
top level: BBa_J23006
start: 258
end: 940
orientation: inline

rrnB T1 terminator
type: SequenceAnnotation
persistent identity: annotation1975724
display id: annotation1975724
version: 1
top level: BBa_J23006
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_J23006/annotation1975724/range1975724/1

https://synbiohub.org/public/igem/BBa_J23006/annotation1975724/range1975724/1
type: Range
persistent identity: range1975724
display id: range1975724
version: 1
direction: direction/forward
top level: BBa_J23006
start: 2169
end: 2212
orientation: inline

pBR322 origin
type: SequenceAnnotation
persistent identity: annotation1975726
display id: annotation1975726
version: 1
top level: BBa_J23006
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J23006/annotation1975726/range1975726/1

https://synbiohub.org/public/igem/BBa_J23006/annotation1975726/range1975726/1
type: Range
persistent identity: range1975726
display id: range1975726
version: 1
direction: direction/forward
top level: BBa_J23006
start: 261
end: 880
orientation: inline

NotI
type: SequenceAnnotation
persistent identity: annotation1975729
display id: annotation1975729
version: 1
top level: BBa_J23006
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J23006/annotation1975729/range1975729/1

https://synbiohub.org/public/igem/BBa_J23006/annotation1975729/range1975729/1
type: Range
persistent identity: range1975729
display id: range1975729
version: 1
direction: direction/forward
top level: BBa_J23006
start: 2142
end: 2149
orientation: inline

beta-lactamase promoter
type: SequenceAnnotation
persistent identity: annotation1975725
display id: annotation1975725
version: 1
top level: BBa_J23006
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J23006/annotation1975725/range1975725/1

https://synbiohub.org/public/igem/BBa_J23006/annotation1975725/range1975725/1
type: Range
persistent identity: range1975725
display id: range1975725
version: 1
direction: direction/reverse
top level: BBa_J23006
start: 1937
end: 1965
orientation: inline

BBa_I732950
type: ComponentDefinition
persistent identity: BBa I732950
display id: BBa_I732950
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: P_template1 + lacZ
creator: Zhan Jian
created: 2007-10-25T11:00:00Z
modified: 2015-08-31T04:08:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Intermediate in the construction of P_template1
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: pBR322 origin
sequence annotation: End of terminator
sequence annotation: beta-lactamase (ampR) promoter
sequence annotation: BioBrick suffix
sequence annotation: Stem loop
sequence annotation: beta-lactamase ampicillin resistance marker
sequence annotation: Ptemplate1?
sequence annotation: Verification reverse (VR) primer binding site
sequence annotation: XbaI
sequence annotation: Deletion of AC
sequence annotation: kanamycin resistance marker
sequence annotation: EcoRI
sequence annotation: E. coli his operon terminator
sequence annotation: Verification forward (VF2) primer binding site
sequence: https://synbiohub.org/public/igem/BBa_I732950_sequence/1

pBR322 origin
type: SequenceAnnotation
persistent identity: annotation1975770
display id: annotation1975770
version: 1
top level: BBa_I732950
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_I732950/annotation1975770/range1975770/1

https://synbiohub.org/public/igem/BBa_I732950/annotation1975770/range1975770/1
type: Range
persistent identity: range1975770
display id: range1975770
version: 1
direction: direction/reverse
top level: BBa_I732950
start: 261
end: 880
orientation: inline

End of terminator
type: SequenceAnnotation
persistent identity: annotation1975783
display id: annotation1975783
version: 1
top level: BBa_I732950
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I732950/annotation1975783/range1975783/1

https://synbiohub.org/public/igem/BBa_I732950/annotation1975783/range1975783/1
type: Range
persistent identity: range1975783
display id: range1975783
version: 1
direction: direction/reverse
top level: BBa_I732950
start: 3126
end: 3129
orientation: inline

beta-lactamase (ampR) promoter
type: SequenceAnnotation
persistent identity: annotation1975773
display id: annotation1975773
version: 1
top level: BBa_I732950
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I732950/annotation1975773/range1975773/1

https://synbiohub.org/public/igem/BBa_I732950/annotation1975773/range1975773/1
type: Range
persistent identity: range1975773
display id: range1975773
version: 1
direction: direction/reverse
top level: BBa_I732950
start: 2969
end: 2997
orientation: inline

BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation1975774
display id: annotation1975774
version: 1
top level: BBa_I732950
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_I732950/annotation1975774/range1975774/1

https://synbiohub.org/public/igem/BBa_I732950/annotation1975774/range1975774/1
type: Range
persistent identity: range1975774
display id: range1975774
version: 1
direction: direction/forward
top level: BBa_I732950
start: 1
end: 21
orientation: inline

Stem loop
type: SequenceAnnotation
persistent identity: annotation1975782
display id: annotation1975782
version: 1
top level: BBa_I732950
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I732950/annotation1975782/range1975782/1

https://synbiohub.org/public/igem/BBa_I732950/annotation1975782/range1975782/1
type: Range
persistent identity: range1975782
display id: range1975782
version: 1
direction: direction/reverse
top level: BBa_I732950
start: 3139
end: 3158
orientation: inline

beta-lactamase ampicillin resistance marker
type: SequenceAnnotation
persistent identity: annotation1975772
display id: annotation1975772
version: 1
top level: BBa_I732950
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I732950/annotation1975772/range1975772/1

https://synbiohub.org/public/igem/BBa_I732950/annotation1975772/range1975772/1
type: Range
persistent identity: range1975772
display id: range1975772
version: 1
direction: direction/reverse
top level: BBa_I732950
start: 2067
end: 2927
orientation: inline

Ptemplate1?
type: SequenceAnnotation
persistent identity: annotation1975784
display id: annotation1975784
version: 1
top level: BBa_I732950
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I732950/annotation1975784/range1975784/1

https://synbiohub.org/public/igem/BBa_I732950/annotation1975784/range1975784/1
type: Range
persistent identity: range1975784
display id: range1975784
version: 1
direction: direction/forward
top level: BBa_I732950
start: 3174
end: 3332
orientation: inline

Stem loop
type: SequenceAnnotation
persistent identity: annotation1975781
display id: annotation1975781
version: 1
top level: BBa_I732950
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I732950/annotation1975781/range1975781/1

https://synbiohub.org/public/igem/BBa_I732950/annotation1975781/range1975781/1
type: Range
persistent identity: range1975781
display id: range1975781
version: 1
direction: direction/forward
top level: BBa_I732950
start: 30
end: 64
orientation: inline

Verification reverse (VR) primer binding site
type: SequenceAnnotation
persistent identity: annotation1975778
display id: annotation1975778
version: 1
top level: BBa_I732950
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_I732950/annotation1975778/range1975778/1

https://synbiohub.org/public/igem/BBa_I732950/annotation1975778/range1975778/1
type: Range
persistent identity: range1975778
display id: range1975778
version: 1
direction: direction/reverse
top level: BBa_I732950
start: 157
end: 176
orientation: inline

XbaI
type: SequenceAnnotation
persistent identity: annotation1975776
display id: annotation1975776
version: 1
top level: BBa_I732950
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_I732950/annotation1975776/range1975776/1

https://synbiohub.org/public/igem/BBa_I732950/annotation1975776/range1975776/1
type: Range
persistent identity: range1975776
display id: range1975776
version: 1
direction: direction/forward
top level: BBa_I732950
start: 3333
end: 3338
orientation: inline

Deletion of AC
type: SequenceAnnotation
persistent identity: annotation1975780
display id: annotation1975780
version: 1
top level: BBa_I732950
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_I732950/annotation1975780/range1975780/1

https://synbiohub.org/public/igem/BBa_I732950/annotation1975780/range1975780/1
type: Range
persistent identity: range1975780
display id: range1975780
version: 1
direction: direction/forward
top level: BBa_I732950
start: 34
end: 35
orientation: inline

kanamycin resistance marker
type: SequenceAnnotation
persistent identity: annotation1975771
display id: annotation1975771
version: 1
top level: BBa_I732950
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I732950/annotation1975771/range1975771/1

https://synbiohub.org/public/igem/BBa_I732950/annotation1975771/range1975771/1
type: Range
persistent identity: range1975771
display id: range1975771
version: 1
direction: direction/reverse
top level: BBa_I732950
start: 1097
end: 1912
orientation: inline

EcoRI
type: SequenceAnnotation
persistent identity: annotation1975775
display id: annotation1975775
version: 1
top level: BBa_I732950
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_I732950/annotation1975775/range1975775/1

https://synbiohub.org/public/igem/BBa_I732950/annotation1975775/range1975775/1
type: Range
persistent identity: range1975775
display id: range1975775
version: 1
direction: direction/forward
top level: BBa_I732950
start: 3168
end: 3173
orientation: inline

E. coli his operon terminator
type: SequenceAnnotation
persistent identity: annotation1975779
display id: annotation1975779
version: 1
top level: BBa_I732950
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I732950/annotation1975779/range1975779/1

https://synbiohub.org/public/igem/BBa_I732950/annotation1975779/range1975779/1
type: Range
persistent identity: range1975779
display id: range1975779
version: 1
direction: direction/forward
top level: BBa_I732950
start: 22
end: 93
orientation: inline

Verification forward (VF2) primer binding site
type: SequenceAnnotation
persistent identity: annotation1975777
display id: annotation1975777
version: 1
top level: BBa_I732950
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_I732950/annotation1975777/range1975777/1

https://synbiohub.org/public/igem/BBa_I732950/annotation1975777/range1975777/1
type: Range
persistent identity: range1975777
display id: range1975777
version: 1
direction: direction/forward
top level: BBa_I732950
start: 3050
end: 3069
orientation: inline