{| border="1" |- |align="center"|'''Genotype''' |align="center"|'''Character''' |align="center"|'''Name''' |- |Top10/pT-TERM |So not express any repressors |align="center"|CR00 |- |Top10/pT-ARL4A0604 |Constitutively express ARL4A0604 |align="center"|CR01 |- |Top10/pT-ARL2A0203 |Constitutively express ARL2A0203 |align="center"|CR10 |- |Top10/pTet-ARL4A0604-ARL2A203 |Constitutively express ARL4A0604 and ARL2A0203 |align="center"|CR11 |}
Verification forward (VF2) primer binding site
beta-lactamase (ampicillin resistance marker)
Verification reverse (VR) primer binding site
Part-making plasmid with Ptet-XbaI-[biobrick]-SpeI-PstI
pSB1A2-I13521 See KAD062806-Construction of J23006 for details
Plasmid for constructing biobrick parts that provides a minimal set of functions to observe activity.
beta-lactamase ampicillin resistance marker
Variant of pBca1021 (J61003) with GFP inserted. In practice, it is a E0040 basic part just in a different pSB1A* plasmid.
* This part is a compination of E1010, B0034 and pSB1C3 from the registry.
This part is similar to [http://partsregistry.org/Part:BBa_J61002 J61002] but the backbone is based on pSB1C3 and carries a chloramphenicol resistance.
pSBBs1C-lacZ (lacZ reporter vector for B. subtilis)
This vector is the Bacillus vector pAC6 brought into the iGEM BioBrick standard.
This part is a backbone vector for Bacillus subtilis. It integrates at the amyE locus. It has a Ampicillin resistance for cloning in E.coli and Chloramphenicol resistance for B.subtilis. The multiple cloning site is followed by a lacZ gene for reporter assays. This BioBrick is part of the LMU 2012 igem project Bacillus BioBrick Box http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks
This reporter vector can be used with the normal BioBrick assembly strategies.
PCR ca1020F/R on pSB1A2-I13521 (900bp, SpeI/PstI) Sub into pSB1A2-I13522 (SpeI/PstI) Product is pBca1020, will be an iGEM number ultimately ---- ca1020F Forward SpeI oligo for X-rbsRFP-P plasmid gcactACTAGTgaaagaggagaaatactagatggc ca1020R Reverse PstI oligo for X-rbsRFP-P plasmid ccggactgcagcggccgcttctagtatataaacg
Plasmid for construction of promoter basic parts. Insertion of a promoter element between XbaI and SpeI sites results in a RFP reporter while retaining the ability to do biobrick assembly. Part J61002 is the tet promoter variant of the plasmid.
PCR KB001/ca1021R on pSB1A2-J01022 (250 bp, EcoRI/XbaI) sub into pSB1A2-E0040(GFP;DNA-1-5H) (EcoRI/XbaI) Product is pBca1021-E0040 ---- ca1021R Reverse b0034 XbaI AGCCATCTAGAATTTCTCCTCTTTCTCTAG KB001 Forward EcoRI to construct Ptet part-making biobrick plasmid ttctggaattcgcggccgcaactagagccaggcatc
Plasmid for assembly of open reading frame basic parts. A Tet promoter (r0040) and strong ribosome binding site (b0034) lie between the EcoRI and XbaI sites. Insertion of an open reading frame basic part into the XbaI and PstI sites results in constitutive expression but retains basic part utilityin standard assembly.
pSBBs3C-luxABCDE (lux reporter vector for B.subtilis)
This vector is an igem compatible version of the pAH328 Bacillus reporter vecotr.
This part is a reporter vector for Bacillus subtilis. It integrates at the sacA locus of B.subtilis genome. It has a ampicillin resistance for cloning in E.coli and Chloramphenicol resistance for selection in B. subtilis. The multiple cloning site has a rfp for selection and is followed by the luxABCDE gene cluster for luminescence reporter assays. This BioBrick is part of the LMU-Munich 2012 igem project Bacillus BioBrickBOX http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks
We removed any forbidden restriction sites by site-directed mutagenesis PCR. The clonging site also includes a rfp for selection for cloning in E.coli.
Variant of pBca1021 (J61003) with GFP inserted. Part J23019 is the same as J23018 but in pSB3C6.
chloramphenicol acetyl transferase (CmR resistance marker)
Intermediate in the construction of P_template1
Conversion of the iGEM parts registry to SBOL2.1