//plasmidbackbone/libraryscreening/promoter
type: Collection
persistent identity: plasmidbackbone libraryscreening promoter
display id: plasmidbackbone_libraryscreening_promoter
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: pSBBs1C
member: p68
member: BBa_K777133
member: BBa_J61002
member: pSBBs3C

//plasmidbackbone/libraryscreening/rbscodingsequence
type: Collection
persistent identity: plasmidbackbone libraryscreening rbscodingsequence
display id: plasmidbackbone_libraryscreening_rbscodingsequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: BBa_I732950
member: BBa_I732952
member: BBa_J23006

//plasmidbackbone/libraryscreening/codingsequence
type: Collection
persistent identity: plasmidbackbone libraryscreening codingsequence
display id: plasmidbackbone_libraryscreening_codingsequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: BBa_J23019
member: BBa_J61003
member: BBa_J23018

//plasmidbackbone/libraryscreening
type: Collection
persistent identity: plasmidbackbone libraryscreening
display id: plasmidbackbone_libraryscreening
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: //plasmidbackbone/libraryscreening/promoter
member: //plasmidbackbone/libraryscreening/rbscodingsequence
member: //plasmidbackbone/libraryscreening/codingsequence

BBa_I732952
type: ComponentDefinition
persistent identity: BBa I732952
display id: BBa_I732952
version: 1
was generated by: iGEM to SBOL conversion
description: Ptemplate1 + RBS.ARL2A0103
created: 2007-10-25T11:00:00Z
modified: 2015-08-31T04:08:00Z
mutable provenance: N/A
owned by: james
owned by: myers
mutable description: {| border="1" |- |align="center"|'''Genotype''' |align="center"|'''Character''' |align="center"|'''Name''' |- |Top10/pT-TERM |So not express any repressors |align="center"|CR00 |- |Top10/pT-ARL4A0604 |Constitutively express ARL4A0604 |align="center"|CR01 |- |Top10/pT-ARL2A0203 |Constitutively express ARL2A0203 |align="center"|CR10 |- |Top10/pTet-ARL4A0604-ARL2A203 |Constitutively express ARL4A0604 and ARL2A0203 |align="center"|CR11 |}
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: N/A
star: false
creator: Zhan Jian
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: XbaI
sequence annotation: E. coli his operon terminator
sequence annotation: Ptemplate1?
sequence annotation: pBR322 origin
sequence annotation: beta-lactamase (ampR) promoter
sequence annotation: Deletion of AC
sequence annotation: Stem loop
sequence annotation: BioBrick suffix
sequence annotation: Verification forward (VF2) primer binding site
sequence annotation: End of terminator
sequence annotation: beta-lactamase (ampicillin resistance marker)
sequence annotation: Verification reverse (VR) primer binding site
sequence annotation: EcoRI
sequence: https://synbiohub.org/public/igem/BBa_I732952_sequence/1

XbaI
type: SequenceAnnotation
persistent identity: annotation1975790
display id: annotation1975790
version: 1
top level: BBa_I732952
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_I732952/annotation1975790/range1975790/1

https://synbiohub.org/public/igem/BBa_I732952/annotation1975790/range1975790/1
type: Range
persistent identity: range1975790
display id: range1975790
version: 1
top level: BBa_I732952
direction: direction/forward
start: 2301
end: 2306
orientation: inline

E. coli his operon terminator
type: SequenceAnnotation
persistent identity: annotation1975794
display id: annotation1975794
version: 1
top level: BBa_I732952
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I732952/annotation1975794/range1975794/1

https://synbiohub.org/public/igem/BBa_I732952/annotation1975794/range1975794/1
type: Range
persistent identity: range1975794
display id: range1975794
version: 1
top level: BBa_I732952
direction: direction/forward
start: 22
end: 93
orientation: inline

Ptemplate1?
type: SequenceAnnotation
persistent identity: annotation1975798
display id: annotation1975798
version: 1
top level: BBa_I732952
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I732952/annotation1975798/range1975798/1

https://synbiohub.org/public/igem/BBa_I732952/annotation1975798/range1975798/1
type: Range
persistent identity: range1975798
display id: range1975798
version: 1
top level: BBa_I732952
direction: direction/forward
start: 2142
end: 2300
orientation: inline

pBR322 origin
type: SequenceAnnotation
persistent identity: annotation1975785
display id: annotation1975785
version: 1
top level: BBa_I732952
role: feature/dna
role: SO:0000110
location: https://synbiohub.org/public/igem/BBa_I732952/annotation1975785/range1975785/1

https://synbiohub.org/public/igem/BBa_I732952/annotation1975785/range1975785/1
type: Range
persistent identity: range1975785
display id: range1975785
version: 1
top level: BBa_I732952
direction: direction/reverse
start: 261
end: 880
orientation: inline

beta-lactamase (ampR) promoter
type: SequenceAnnotation
persistent identity: annotation1975787
display id: annotation1975787
version: 1
top level: BBa_I732952
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I732952/annotation1975787/range1975787/1

https://synbiohub.org/public/igem/BBa_I732952/annotation1975787/range1975787/1
type: Range
persistent identity: range1975787
display id: range1975787
version: 1
top level: BBa_I732952
direction: direction/reverse
start: 1937
end: 1965
orientation: inline

Deletion of AC
type: SequenceAnnotation
persistent identity: annotation1975796
display id: annotation1975796
version: 1
top level: BBa_I732952
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_I732952/annotation1975796/range1975796/1

https://synbiohub.org/public/igem/BBa_I732952/annotation1975796/range1975796/1
type: Range
persistent identity: range1975796
display id: range1975796
version: 1
top level: BBa_I732952
direction: direction/forward
start: 34
end: 35
orientation: inline

Stem loop
type: SequenceAnnotation
persistent identity: annotation1975795
display id: annotation1975795
version: 1
top level: BBa_I732952
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I732952/annotation1975795/range1975795/1

https://synbiohub.org/public/igem/BBa_I732952/annotation1975795/range1975795/1
type: Range
persistent identity: range1975795
display id: range1975795
version: 1
top level: BBa_I732952
direction: direction/forward
start: 30
end: 64
orientation: inline

BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation1975789
display id: annotation1975789
version: 1
top level: BBa_I732952
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_I732952/annotation1975789/range1975789/1

https://synbiohub.org/public/igem/BBa_I732952/annotation1975789/range1975789/1
type: Range
persistent identity: range1975789
display id: range1975789
version: 1
top level: BBa_I732952
direction: direction/forward
start: 1
end: 21
orientation: inline

Verification forward (VF2) primer binding site
type: SequenceAnnotation
persistent identity: annotation1975791
display id: annotation1975791
version: 1
top level: BBa_I732952
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_I732952/annotation1975791/range1975791/1

https://synbiohub.org/public/igem/BBa_I732952/annotation1975791/range1975791/1
type: Range
persistent identity: range1975791
display id: range1975791
version: 1
top level: BBa_I732952
direction: direction/forward
start: 2018
end: 2037
orientation: inline

End of terminator
type: SequenceAnnotation
persistent identity: annotation1975793
display id: annotation1975793
version: 1
top level: BBa_I732952
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I732952/annotation1975793/range1975793/1

https://synbiohub.org/public/igem/BBa_I732952/annotation1975793/range1975793/1
type: Range
persistent identity: range1975793
display id: range1975793
version: 1
top level: BBa_I732952
direction: direction/reverse
start: 2094
end: 2097
orientation: inline

Stem loop
type: SequenceAnnotation
persistent identity: annotation1975792
display id: annotation1975792
version: 1
top level: BBa_I732952
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I732952/annotation1975792/range1975792/1

https://synbiohub.org/public/igem/BBa_I732952/annotation1975792/range1975792/1
type: Range
persistent identity: range1975792
display id: range1975792
version: 1
top level: BBa_I732952
direction: direction/reverse
start: 2107
end: 2126
orientation: inline

beta-lactamase (ampicillin resistance marker)
type: SequenceAnnotation
persistent identity: annotation1975786
display id: annotation1975786
version: 1
top level: BBa_I732952
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I732952/annotation1975786/range1975786/1

https://synbiohub.org/public/igem/BBa_I732952/annotation1975786/range1975786/1
type: Range
persistent identity: range1975786
display id: range1975786
version: 1
top level: BBa_I732952
direction: direction/reverse
start: 1035
end: 1895
orientation: inline

Verification reverse (VR) primer binding site
type: SequenceAnnotation
persistent identity: annotation1975797
display id: annotation1975797
version: 1
top level: BBa_I732952
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_I732952/annotation1975797/range1975797/1

https://synbiohub.org/public/igem/BBa_I732952/annotation1975797/range1975797/1
type: Range
persistent identity: range1975797
display id: range1975797
version: 1
top level: BBa_I732952
direction: direction/reverse
start: 157
end: 176
orientation: inline

EcoRI
type: SequenceAnnotation
persistent identity: annotation1975788
display id: annotation1975788
version: 1
top level: BBa_I732952
role: feature/dna
role: SO:0000110
location: https://synbiohub.org/public/igem/BBa_I732952/annotation1975788/range1975788/1

https://synbiohub.org/public/igem/BBa_I732952/annotation1975788/range1975788/1
type: Range
persistent identity: range1975788
display id: range1975788
version: 1
top level: BBa_I732952
direction: direction/forward
start: 2136
end: 2141
orientation: inline

BBa_J23006
type: ComponentDefinition
persistent identity: BBa J23006
display id: BBa_J23006
version: 1
was generated by: iGEM to SBOL conversion
description: Part-making plasmid with Ptet-XbaI-[biobrick]-SpeI-PstI
created: 2006-06-30T11:00:00Z
modified: 2015-08-31T04:08:38Z
mutable provenance: pSB1A2-I13521 See KAD062806-Construction of J23006 for details
owned by: james
owned by: myers
mutable description: Plasmid for constructing biobrick parts that provides a minimal set of functions to observe activity.
discontinued: false
dominant: false
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: EcoRI
sequence annotation: BBa_B0015 terminator
sequence annotation: beta-lactamase promoter
sequence annotation: XbaI
sequence annotation: ColE1 origin
sequence annotation: NotI
sequence annotation: BioBrick suffix
sequence annotation: R0040 (pTet promoter)
sequence annotation: pBR322 origin
sequence annotation: rrnB T1 terminator
sequence annotation: beta-lactamase ampicillin resistance marker
sequence: https://synbiohub.org/public/igem/BBa_J23006_sequence/1

EcoRI
type: SequenceAnnotation
persistent identity: annotation1975728
display id: annotation1975728
version: 1
top level: BBa_J23006
role: feature/dna
role: SO:0000110
location: https://synbiohub.org/public/igem/BBa_J23006/annotation1975728/range1975728/1

https://synbiohub.org/public/igem/BBa_J23006/annotation1975728/range1975728/1
type: Range
persistent identity: range1975728
display id: range1975728
version: 1
top level: BBa_J23006
direction: direction/forward
start: 2136
end: 2141
orientation: inline

BBa_B0015 terminator
type: SequenceAnnotation
persistent identity: annotation1975722
display id: annotation1975722
version: 1
top level: BBa_J23006
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J23006/annotation1975722/range1975722/1

https://synbiohub.org/public/igem/BBa_J23006/annotation1975722/range1975722/1
type: Range
persistent identity: range1975722
display id: range1975722
version: 1
top level: BBa_J23006
direction: direction/forward
start: 2158
end: 2286
orientation: inline

beta-lactamase promoter
type: SequenceAnnotation
persistent identity: annotation1975725
display id: annotation1975725
version: 1
top level: BBa_J23006
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J23006/annotation1975725/range1975725/1

https://synbiohub.org/public/igem/BBa_J23006/annotation1975725/range1975725/1
type: Range
persistent identity: range1975725
display id: range1975725
version: 1
top level: BBa_J23006
direction: direction/reverse
start: 1937
end: 1965
orientation: inline

XbaI
type: SequenceAnnotation
persistent identity: annotation1975730
display id: annotation1975730
version: 1
top level: BBa_J23006
role: feature/dna
role: SO:0000110
location: https://synbiohub.org/public/igem/BBa_J23006/annotation1975730/range1975730/1

https://synbiohub.org/public/igem/BBa_J23006/annotation1975730/range1975730/1
type: Range
persistent identity: range1975730
display id: range1975730
version: 1
top level: BBa_J23006
direction: direction/forward
start: 2350
end: 2355
orientation: inline

ColE1 origin
type: SequenceAnnotation
persistent identity: annotation1975721
display id: annotation1975721
version: 1
top level: BBa_J23006
role: feature/dna
role: SO:0000110
location: https://synbiohub.org/public/igem/BBa_J23006/annotation1975721/range1975721/1

https://synbiohub.org/public/igem/BBa_J23006/annotation1975721/range1975721/1
type: Range
persistent identity: range1975721
display id: range1975721
version: 1
top level: BBa_J23006
direction: direction/reverse
start: 258
end: 940
orientation: inline

NotI
type: SequenceAnnotation
persistent identity: annotation1975729
display id: annotation1975729
version: 1
top level: BBa_J23006
role: feature/dna
role: SO:0000110
location: https://synbiohub.org/public/igem/BBa_J23006/annotation1975729/range1975729/1

https://synbiohub.org/public/igem/BBa_J23006/annotation1975729/range1975729/1
type: Range
persistent identity: range1975729
display id: range1975729
version: 1
top level: BBa_J23006
direction: direction/forward
start: 2142
end: 2149
orientation: inline

BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation1975727
display id: annotation1975727
version: 1
top level: BBa_J23006
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J23006/annotation1975727/range1975727/1

https://synbiohub.org/public/igem/BBa_J23006/annotation1975727/range1975727/1
type: Range
persistent identity: range1975727
display id: range1975727
version: 1
top level: BBa_J23006
direction: direction/forward
start: 1
end: 21
orientation: inline

R0040 (pTet promoter)
type: SequenceAnnotation
persistent identity: annotation1975720
display id: annotation1975720
version: 1
top level: BBa_J23006
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J23006/annotation1975720/range1975720/1

https://synbiohub.org/public/igem/BBa_J23006/annotation1975720/range1975720/1
type: Range
persistent identity: range1975720
display id: range1975720
version: 1
top level: BBa_J23006
direction: direction/forward
start: 2295
end: 2348
orientation: inline

pBR322 origin
type: SequenceAnnotation
persistent identity: annotation1975726
display id: annotation1975726
version: 1
top level: BBa_J23006
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J23006/annotation1975726/range1975726/1

https://synbiohub.org/public/igem/BBa_J23006/annotation1975726/range1975726/1
type: Range
persistent identity: range1975726
display id: range1975726
version: 1
top level: BBa_J23006
direction: direction/forward
start: 261
end: 880
orientation: inline

rrnB T1 terminator
type: SequenceAnnotation
persistent identity: annotation1975724
display id: annotation1975724
version: 1
top level: BBa_J23006
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_J23006/annotation1975724/range1975724/1

https://synbiohub.org/public/igem/BBa_J23006/annotation1975724/range1975724/1
type: Range
persistent identity: range1975724
display id: range1975724
version: 1
top level: BBa_J23006
direction: direction/forward
start: 2169
end: 2212
orientation: inline

beta-lactamase ampicillin resistance marker
type: SequenceAnnotation
persistent identity: annotation1975723
display id: annotation1975723
version: 1
top level: BBa_J23006
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J23006/annotation1975723/range1975723/1

https://synbiohub.org/public/igem/BBa_J23006/annotation1975723/range1975723/1
type: Range
persistent identity: range1975723
display id: range1975723
version: 1
top level: BBa_J23006
direction: direction/reverse
start: 1035
end: 1895
orientation: inline

BBa_J23018
type: ComponentDefinition
persistent identity: BBa J23018
display id: BBa_J23018
version: 1
was generated by: iGEM to SBOL conversion
description: EN-Ptet-rbs-X-[RFP]-SNP "ORF Expresser"
created: 2006-08-02T11:00:00Z
modified: 2015-08-31T04:08:39Z
mutable provenance: J61003 and E0040
owned by: james
owned by: myers
mutable description: Variant of pBca1021 (J61003) with GFP inserted. In practice, it is a E0040 basic part just in a different pSB1A* plasmid.
discontinued: false
dominant: false
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Verification reverse (VR) primer binding site
sequence annotation: beta-lactamase (ampicillin resistance marker)
sequence annotation: BioBrick suffix
sequence annotation: Ptet
sequence annotation: RBS
sequence annotation: EcoRI
sequence annotation: XbaI
sequence annotation: BBa_B0015 terminator
sequence annotation: pBR322 origin
sequence annotation: rrnB T1 terminator
sequence annotation: BBa_R0040 (Ptet)
sequence annotation: BBa_B0034
sequence annotation: beta-lactamase (ampR) promoter
sequence annotation: NotI
sequence annotation: Verification forward (VF2) primer binding site
sequence annotation: pMB1 origin
sequence: https://synbiohub.org/public/igem/BBa_J23018_sequence/1

Verification reverse (VR) primer binding site
type: SequenceAnnotation
persistent identity: annotation1975761
display id: annotation1975761
version: 1
top level: BBa_J23018
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J23018/annotation1975761/range1975761/1

https://synbiohub.org/public/igem/BBa_J23018/annotation1975761/range1975761/1
type: Range
persistent identity: range1975761
display id: range1975761
version: 1
top level: BBa_J23018
direction: direction/reverse
start: 79
end: 98
orientation: inline

beta-lactamase (ampicillin resistance marker)
type: SequenceAnnotation
persistent identity: annotation1975734
display id: annotation1975734
version: 1
top level: BBa_J23018
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J23018/annotation1975734/range1975734/1

https://synbiohub.org/public/igem/BBa_J23018/annotation1975734/range1975734/1
type: Range
persistent identity: range1975734
display id: range1975734
version: 1
top level: BBa_J23018
direction: direction/reverse
start: 957
end: 1817
orientation: inline

BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation1975737
display id: annotation1975737
version: 1
top level: BBa_J23018
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J23018/annotation1975737/range1975737/1

https://synbiohub.org/public/igem/BBa_J23018/annotation1975737/range1975737/1
type: Range
persistent identity: range1975737
display id: range1975737
version: 1
top level: BBa_J23018
direction: direction/forward
start: 1
end: 21
orientation: inline

Ptet
type: SequenceAnnotation
persistent identity: annotation1975765
display id: annotation1975765
version: 1
top level: BBa_J23018
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J23018/annotation1975765/range1975765/1

https://synbiohub.org/public/igem/BBa_J23018/annotation1975765/range1975765/1
type: Range
persistent identity: range1975765
display id: range1975765
version: 1
top level: BBa_J23018
direction: direction/forward
start: 2217
end: 2270
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1975764
display id: annotation1975764
version: 1
top level: BBa_J23018
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J23018/annotation1975764/range1975764/1

https://synbiohub.org/public/igem/BBa_J23018/annotation1975764/range1975764/1
type: Range
persistent identity: range1975764
display id: range1975764
version: 1
top level: BBa_J23018
direction: direction/forward
start: 2279
end: 2290
orientation: inline

EcoRI
type: SequenceAnnotation
persistent identity: annotation1975738
display id: annotation1975738
version: 1
top level: BBa_J23018
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J23018/annotation1975738/range1975738/1

https://synbiohub.org/public/igem/BBa_J23018/annotation1975738/range1975738/1
type: Range
persistent identity: range1975738
display id: range1975738
version: 1
top level: BBa_J23018
direction: direction/forward
start: 2058
end: 2063
orientation: inline

XbaI
type: SequenceAnnotation
persistent identity: annotation1975740
display id: annotation1975740
version: 1
top level: BBa_J23018
role: feature/dna
role: SO:0000110
location: https://synbiohub.org/public/igem/BBa_J23018/annotation1975740/range1975740/1

https://synbiohub.org/public/igem/BBa_J23018/annotation1975740/range1975740/1
type: Range
persistent identity: range1975740
display id: range1975740
version: 1
top level: BBa_J23018
direction: direction/forward
start: 2292
end: 2297
orientation: inline

BBa_B0015 terminator
type: SequenceAnnotation
persistent identity: annotation1893435
display id: annotation1893435
version: 1
top level: BBa_J23018
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J23018/annotation1893435/range1893435/1

https://synbiohub.org/public/igem/BBa_J23018/annotation1893435/range1893435/1
type: Range
persistent identity: range1893435
display id: range1893435
version: 1
top level: BBa_J23018
direction: direction/forward
start: 2080
end: 2208
orientation: inline

pBR322 origin
type: SequenceAnnotation
persistent identity: annotation1893437
display id: annotation1893437
version: 1
top level: BBa_J23018
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J23018/annotation1893437/range1893437/1

https://synbiohub.org/public/igem/BBa_J23018/annotation1893437/range1893437/1
type: Range
persistent identity: range1893437
display id: range1893437
version: 1
top level: BBa_J23018
direction: direction/reverse
start: 183
end: 802
orientation: inline

rrnB T1 terminator
type: SequenceAnnotation
persistent identity: annotation1975736
display id: annotation1975736
version: 1
top level: BBa_J23018
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_J23018/annotation1975736/range1975736/1

https://synbiohub.org/public/igem/BBa_J23018/annotation1975736/range1975736/1
type: Range
persistent identity: range1975736
display id: range1975736
version: 1
top level: BBa_J23018
direction: direction/forward
start: 2091
end: 2134
orientation: inline

BBa_R0040 (Ptet)
type: SequenceAnnotation
persistent identity: annotation1893436
display id: annotation1893436
version: 1
top level: BBa_J23018
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J23018/annotation1893436/range1893436/1

https://synbiohub.org/public/igem/BBa_J23018/annotation1893436/range1893436/1
type: Range
persistent identity: range1893436
display id: range1893436
version: 1
top level: BBa_J23018
direction: direction/forward
start: 2217
end: 2270
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1975763
display id: annotation1975763
version: 1
top level: BBa_J23018
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J23018/annotation1975763/range1975763/1

https://synbiohub.org/public/igem/BBa_J23018/annotation1975763/range1975763/1
type: Range
persistent identity: range1975763
display id: range1975763
version: 1
top level: BBa_J23018
direction: direction/forward
start: 2279
end: 2290
orientation: inline

beta-lactamase (ampR) promoter
type: SequenceAnnotation
persistent identity: annotation1975735
display id: annotation1975735
version: 1
top level: BBa_J23018
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J23018/annotation1975735/range1975735/1

https://synbiohub.org/public/igem/BBa_J23018/annotation1975735/range1975735/1
type: Range
persistent identity: range1975735
display id: range1975735
version: 1
top level: BBa_J23018
direction: direction/reverse
start: 1859
end: 1887
orientation: inline

NotI
type: SequenceAnnotation
persistent identity: annotation1975739
display id: annotation1975739
version: 1
top level: BBa_J23018
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J23018/annotation1975739/range1975739/1

https://synbiohub.org/public/igem/BBa_J23018/annotation1975739/range1975739/1
type: Range
persistent identity: range1975739
display id: range1975739
version: 1
top level: BBa_J23018
direction: direction/forward
start: 2064
end: 2071
orientation: inline

Verification forward (VF2) primer binding site
type: SequenceAnnotation
persistent identity: annotation1975760
display id: annotation1975760
version: 1
top level: BBa_J23018
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J23018/annotation1975760/range1975760/1

https://synbiohub.org/public/igem/BBa_J23018/annotation1975760/range1975760/1
type: Range
persistent identity: range1975760
display id: range1975760
version: 1
top level: BBa_J23018
direction: direction/forward
start: 1940
end: 1959
orientation: inline

pMB1 origin
type: SequenceAnnotation
persistent identity: annotation1934517
display id: annotation1934517
version: 1
top level: BBa_J23018
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J23018/annotation1934517/range1934517/1

https://synbiohub.org/public/igem/BBa_J23018/annotation1934517/range1934517/1
type: Range
persistent identity: range1934517
display id: range1934517
version: 1
top level: BBa_J23018
direction: direction/reverse
start: 180
end: 895
orientation: inline

BBa_K777133
type: ComponentDefinition
persistent identity: BBa K777133
display id: BBa_K777133
version: 1
was generated by: iGEM to SBOL conversion
description: Similar to pSB1C3, with RFP reporter
created: 2012-09-27T11:00:00Z
modified: 2015-05-08T01:13:17Z
mutable provenance: * This part is a compination of E1010, B0034 and pSB1C3 from the registry.
owned by: james
owned by: myers
mutable description: This part is similar to [http://partsregistry.org/Part:BBa_J61002 J61002] but the backbone is based on pSB1C3 and carries a chloramphenicol resistance.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: * No specific design considerations.
star: false
creator: Team Goettingen
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: PstI
sequence annotation: XbaI
sequence annotation: Chloramphenicol resistance
sequence annotation: SpeI
sequence annotation: EcoRI
sequence annotation: E1010
sequence annotation: VF2 primer binding site
sequence annotation: mRFP1
sequence annotation: RBS
sequence annotation: rep (pMB1)
sequence annotation: BB0034
sequence annotation: VR primer binding site
sequence annotation: ORI
sequence: https://synbiohub.org/public/igem/BBa_K777133_sequence/1

PstI
type: SequenceAnnotation
persistent identity: annotation2203666
display id: annotation2203666
version: 1
top level: BBa_K777133
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K777133/annotation2203666/range2203666/1

https://synbiohub.org/public/igem/BBa_K777133/annotation2203666/range2203666/1
type: Range
persistent identity: range2203666
display id: range2203666
version: 1
top level: BBa_K777133
direction: direction/forward
start: 884
end: 889
orientation: inline

XbaI
type: SequenceAnnotation
persistent identity: annotation2203668
display id: annotation2203668
version: 1
top level: BBa_K777133
role: feature/dna
role: SO:0000110
location: https://synbiohub.org/public/igem/BBa_K777133/annotation2203668/range2203668/1

https://synbiohub.org/public/igem/BBa_K777133/annotation2203668/range2203668/1
type: Range
persistent identity: range2203668
display id: range2203668
version: 1
top level: BBa_K777133
direction: direction/forward
start: 2932
end: 2937
orientation: inline

Chloramphenicol resistance
type: SequenceAnnotation
persistent identity: annotation2203657
display id: annotation2203657
version: 1
top level: BBa_K777133
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K777133/annotation2203657/range2203657/1

https://synbiohub.org/public/igem/BBa_K777133/annotation2203657/range2203657/1
type: Range
persistent identity: range2203657
display id: range2203657
version: 1
top level: BBa_K777133
direction: direction/reverse
start: 2028
end: 2687
orientation: inline

SpeI
type: SequenceAnnotation
persistent identity: annotation2203665
display id: annotation2203665
version: 1
top level: BBa_K777133
role: feature/dna
role: SO:0000110
location: https://synbiohub.org/public/igem/BBa_K777133/annotation2203665/range2203665/1

https://synbiohub.org/public/igem/BBa_K777133/annotation2203665/range2203665/1
type: Range
persistent identity: range2203665
display id: range2203665
version: 1
top level: BBa_K777133
direction: direction/forward
start: 1
end: 6
orientation: inline

EcoRI
type: SequenceAnnotation
persistent identity: annotation2203667
display id: annotation2203667
version: 1
top level: BBa_K777133
role: feature/dna
role: SO:0000110
location: https://synbiohub.org/public/igem/BBa_K777133/annotation2203667/range2203667/1

https://synbiohub.org/public/igem/BBa_K777133/annotation2203667/range2203667/1
type: Range
persistent identity: range2203667
display id: range2203667
version: 1
top level: BBa_K777133
direction: direction/forward
start: 2917
end: 2922
orientation: inline

E1010
type: SequenceAnnotation
persistent identity: annotation2203654
display id: annotation2203654
version: 1
top level: BBa_K777133
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K777133/annotation2203654/range2203654/1

https://synbiohub.org/public/igem/BBa_K777133/annotation2203654/range2203654/1
type: Range
persistent identity: range2203654
display id: range2203654
version: 1
top level: BBa_K777133
direction: direction/forward
start: 26
end: 703
orientation: inline

VF2 primer binding site
type: SequenceAnnotation
persistent identity: annotation2203664
display id: annotation2203664
version: 1
top level: BBa_K777133
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K777133/annotation2203664/range2203664/1

https://synbiohub.org/public/igem/BBa_K777133/annotation2203664/range2203664/1
type: Range
persistent identity: range2203664
display id: range2203664
version: 1
top level: BBa_K777133
direction: direction/forward
start: 2799
end: 2818
orientation: inline

mRFP1
type: SequenceAnnotation
persistent identity: annotation2203653
display id: annotation2203653
version: 1
top level: BBa_K777133
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K777133/annotation2203653/range2203653/1

https://synbiohub.org/public/igem/BBa_K777133/annotation2203653/range2203653/1
type: Range
persistent identity: range2203653
display id: range2203653
version: 1
top level: BBa_K777133
direction: direction/forward
start: 26
end: 703
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2203651
display id: annotation2203651
version: 1
top level: BBa_K777133
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K777133/annotation2203651/range2203651/1

https://synbiohub.org/public/igem/BBa_K777133/annotation2203651/range2203651/1
type: Range
persistent identity: range2203651
display id: range2203651
version: 1
top level: BBa_K777133
direction: direction/forward
start: 8
end: 19
orientation: inline

rep (pMB1)
type: SequenceAnnotation
persistent identity: annotation2203655
display id: annotation2203655
version: 1
top level: BBa_K777133
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K777133/annotation2203655/range2203655/1

https://synbiohub.org/public/igem/BBa_K777133/annotation2203655/range2203655/1
type: Range
persistent identity: range2203655
display id: range2203655
version: 1
top level: BBa_K777133
direction: direction/forward
start: 1129
end: 1735
orientation: inline

BB0034
type: SequenceAnnotation
persistent identity: annotation2203652
display id: annotation2203652
version: 1
top level: BBa_K777133
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K777133/annotation2203652/range2203652/1

https://synbiohub.org/public/igem/BBa_K777133/annotation2203652/range2203652/1
type: Range
persistent identity: range2203652
display id: range2203652
version: 1
top level: BBa_K777133
direction: direction/forward
start: 8
end: 19
orientation: inline

VR primer binding site
type: SequenceAnnotation
persistent identity: annotation2203663
display id: annotation2203663
version: 1
top level: BBa_K777133
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K777133/annotation2203663/range2203663/1

https://synbiohub.org/public/igem/BBa_K777133/annotation2203663/range2203663/1
type: Range
persistent identity: range2203663
display id: range2203663
version: 1
top level: BBa_K777133
direction: direction/forward
start: 1023
end: 1042
orientation: inline

ORI
type: SequenceAnnotation
persistent identity: annotation2203662
display id: annotation2203662
version: 1
top level: BBa_K777133
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K777133/annotation2203662/range2203662/1

https://synbiohub.org/public/igem/BBa_K777133/annotation2203662/range2203662/1
type: Range
persistent identity: range2203662
display id: range2203662
version: 1
top level: BBa_K777133
direction: direction/forward
start: 1144
end: 1144
orientation: inline

p68
type: ComponentDefinition
persistent identity: BBa K678070
display id: BBa_K678070
version: 1
was generated by: iGEM to SBOL conversion
description: p68
created: 2011-09-12T11:00:00Z
modified: 2015-05-08T01:13:05Z
mutable provenance: To be announced
owned by: james
owned by: myers
mutable description: To be announced
discontinued: false
dominant: false
experience: experience/Works
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: To be announced
star: false
creator: DTU-Denmark-2
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
sequence annotation: USER cassette (AsiSI/Nb.BtsI)
sequence annotation: IS-down
sequence annotation: ampR
sequence annotation: TtrpC
sequence annotation: lacZ
sequence annotation: IS-up
sequence annotation: argB marker cassette
sequence: https://synbiohub.org/public/igem/BBa_K678070_sequence/1

USER cassette (AsiSI/Nb.BtsI)
type: SequenceAnnotation
persistent identity: annotation2135107
display id: annotation2135107
version: 1
top level: p68
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678070/annotation2135107/range2135107/1

https://synbiohub.org/public/igem/BBa_K678070/annotation2135107/range2135107/1
type: Range
persistent identity: range2135107
display id: range2135107
version: 1
top level: p68
direction: direction/forward
start: 3285
end: 3318
orientation: inline

IS-down
type: SequenceAnnotation
persistent identity: annotation2135111
display id: annotation2135111
version: 1
top level: p68
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678070/annotation2135111/range2135111/1

https://synbiohub.org/public/igem/BBa_K678070/annotation2135111/range2135111/1
type: Range
persistent identity: range2135111
display id: range2135111
version: 1
top level: p68
direction: direction/forward
start: 9342
end: 11267
orientation: inline

ampR
type: SequenceAnnotation
persistent identity: annotation2135105
display id: annotation2135105
version: 1
top level: p68
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678070/annotation2135105/range2135105/1

https://synbiohub.org/public/igem/BBa_K678070/annotation2135105/range2135105/1
type: Range
persistent identity: range2135105
display id: range2135105
version: 1
top level: p68
direction: direction/forward
start: 1
end: 859
orientation: inline

TtrpC
type: SequenceAnnotation
persistent identity: annotation2135109
display id: annotation2135109
version: 1
top level: p68
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K678070/annotation2135109/range2135109/1

https://synbiohub.org/public/igem/BBa_K678070/annotation2135109/range2135109/1
type: Range
persistent identity: range2135109
display id: range2135109
version: 1
top level: p68
direction: direction/forward
start: 6396
end: 7157
orientation: inline

lacZ
type: SequenceAnnotation
persistent identity: annotation2135108
display id: annotation2135108
version: 1
top level: p68
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K678070/annotation2135108/range2135108/1

https://synbiohub.org/public/igem/BBa_K678070/annotation2135108/range2135108/1
type: Range
persistent identity: range2135108
display id: range2135108
version: 1
top level: p68
direction: direction/forward
start: 3319
end: 6392
orientation: inline

IS-up
type: SequenceAnnotation
persistent identity: annotation2135106
display id: annotation2135106
version: 1
top level: p68
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678070/annotation2135106/range2135106/1

https://synbiohub.org/public/igem/BBa_K678070/annotation2135106/range2135106/1
type: Range
persistent identity: range2135106
display id: range2135106
version: 1
top level: p68
direction: direction/forward
start: 1318
end: 3266
orientation: inline

argB marker cassette
type: SequenceAnnotation
persistent identity: annotation2135110
display id: annotation2135110
version: 1
top level: p68
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678070/annotation2135110/range2135110/1

https://synbiohub.org/public/igem/BBa_K678070/annotation2135110/range2135110/1
type: Range
persistent identity: range2135110
display id: range2135110
version: 1
top level: p68
direction: direction/forward
start: 7171
end: 9327
orientation: inline

pSBBs1C
type: ComponentDefinition
persistent identity: BBa K823021
display id: BBa_K823021
version: 1
was generated by: iGEM to SBOL conversion
description: pSBBs1C-lacZ (lacZ reporter vector for B. subtilis)
created: 2012-08-15T11:00:00Z
modified: 2015-05-08T01:13:30Z
mutable provenance: This vector is the Bacillus vector pAC6 brought into the iGEM BioBrick standard.
owned by: james
owned by: myers
mutable description: This part is a backbone vector for Bacillus subtilis. It integrates at the amyE locus. It has a Ampicillin resistance for cloning in E.coli and Chloramphenicol resistance for B.subtilis. The multiple cloning site is followed by a lacZ gene for reporter assays. This BioBrick is part of the LMU 2012 igem project Bacillus BioBrick Box http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 0
owner id: 6378
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: This reporter vector can be used with the normal BioBrick assembly strategies.
star: false
creator: Jara Radeck
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: cat
sequence annotation: lacZ
sequence annotation: bla
sequence annotation: amyE'
sequence annotation: 'amyE
sequence: https://synbiohub.org/public/igem/BBa_K823021_sequence/1

cat
type: SequenceAnnotation
persistent identity: annotation2179606
display id: annotation2179606
version: 1
top level: pSBBs1C
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823021/annotation2179606/range2179606/1

https://synbiohub.org/public/igem/BBa_K823021/annotation2179606/range2179606/1
type: Range
persistent identity: range2179606
display id: range2179606
version: 1
top level: pSBBs1C
direction: direction/reverse
start: 8813
end: 9463
orientation: inline

lacZ
type: SequenceAnnotation
persistent identity: annotation2179602
display id: annotation2179602
version: 1
top level: pSBBs1C
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823021/annotation2179602/range2179602/1

https://synbiohub.org/public/igem/BBa_K823021/annotation2179602/range2179602/1
type: Range
persistent identity: range2179602
display id: range2179602
version: 1
top level: pSBBs1C
direction: direction/forward
start: 49
end: 3112
orientation: inline

bla
type: SequenceAnnotation
persistent identity: annotation2179604
display id: annotation2179604
version: 1
top level: pSBBs1C
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823021/annotation2179604/range2179604/1

https://synbiohub.org/public/igem/BBa_K823021/annotation2179604/range2179604/1
type: Range
persistent identity: range2179604
display id: range2179604
version: 1
top level: pSBBs1C
direction: direction/forward
start: 4023
end: 4883
orientation: inline

amyE'
type: SequenceAnnotation
persistent identity: annotation2179603
display id: annotation2179603
version: 1
top level: pSBBs1C
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823021/annotation2179603/range2179603/1

https://synbiohub.org/public/igem/BBa_K823021/annotation2179603/range2179603/1
type: Range
persistent identity: range2179603
display id: range2179603
version: 1
top level: pSBBs1C
direction: direction/reverse
start: 3165
end: 3698
orientation: inline

'amyE
type: SequenceAnnotation
persistent identity: annotation2179605
display id: annotation2179605
version: 1
top level: pSBBs1C
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823021/annotation2179605/range2179605/1

https://synbiohub.org/public/igem/BBa_K823021/annotation2179605/range2179605/1
type: Range
persistent identity: range2179605
display id: range2179605
version: 1
top level: pSBBs1C
direction: direction/reverse
start: 7402
end: 8229
orientation: inline

BBa_J61002
type: ComponentDefinition
persistent identity: BBa J61002
display id: BBa_J61002
version: 1
was generated by: iGEM to SBOL conversion
description: EX-Ptet-S-rbsRFP-P "RFP reporter"
created: 2006-07-30T11:00:00Z
modified: 2015-08-31T02:02:59Z
mutable provenance: PCR ca1020F/R on pSB1A2-I13521 (900bp, SpeI/PstI) Sub into pSB1A2-I13522 (SpeI/PstI) Product is pBca1020, will be an iGEM number ultimately ---- ca1020F Forward SpeI oligo for X-rbsRFP-P plasmid gcactACTAGTgaaagaggagaaatactagatggc ca1020R Reverse PstI oligo for X-rbsRFP-P plasmid ccggactgcagcggccgcttctagtatataaacg
owned by: james
owned by: myers
mutable description: Plasmid for construction of promoter basic parts. Insertion of a promoter element between XbaI and SpeI sites results in a RFP reporter while retaining the ability to do biobrick assembly. Part J61002 is the tet promoter variant of the plasmid.
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: SpeI
sequence annotation: Verification forward (VF2) primer binding site
sequence annotation: BBa_B0034 RBS
sequence annotation: beta-lactamase ampicillin resistance marker
sequence annotation: RBS
sequence annotation: mRFP
sequence annotation: NotI
sequence annotation: PstI
sequence annotation: pBR322 origin
sequence annotation: ColE1 origin
sequence annotation: beta-lactamase (ampR, bla) promoter
sequence annotation: BBa_B0015
sequence annotation: Verification reverse (VR) primer binding site
sequence annotation: BioBrick prefix
sequence annotation: Ambiguity; should be a T
sequence annotation: rrnB terminator
sequence: https://synbiohub.org/public/igem/BBa_J61002_sequence/1

SpeI
type: SequenceAnnotation
persistent identity: annotation1975716
display id: annotation1975716
version: 1
top level: BBa_J61002
role: feature/dna
role: SO:0000110
location: https://synbiohub.org/public/igem/BBa_J61002/annotation1975716/range1975716/1

https://synbiohub.org/public/igem/BBa_J61002/annotation1975716/range1975716/1
type: Range
persistent identity: range1975716
display id: range1975716
version: 1
top level: BBa_J61002
direction: direction/forward
start: 2
end: 7
orientation: inline

Verification forward (VF2) primer binding site
type: SequenceAnnotation
persistent identity: annotation1975769
display id: annotation1975769
version: 1
top level: BBa_J61002
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J61002/annotation1975769/range1975769/1

https://synbiohub.org/public/igem/BBa_J61002/annotation1975769/range1975769/1
type: Range
persistent identity: range1975769
display id: range1975769
version: 1
top level: BBa_J61002
direction: direction/forward
start: 2809
end: 2828
orientation: inline

BBa_B0034 RBS
type: SequenceAnnotation
persistent identity: annotation1975767
display id: annotation1975767
version: 1
top level: BBa_J61002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J61002/annotation1975767/range1975767/1

https://synbiohub.org/public/igem/BBa_J61002/annotation1975767/range1975767/1
type: Range
persistent identity: range1975767
display id: range1975767
version: 1
top level: BBa_J61002
direction: direction/forward
start: 9
end: 20
orientation: inline

beta-lactamase ampicillin resistance marker
type: SequenceAnnotation
persistent identity: annotation1892276
display id: annotation1892276
version: 1
top level: BBa_J61002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J61002/annotation1892276/range1892276/1

https://synbiohub.org/public/igem/BBa_J61002/annotation1892276/range1892276/1
type: Range
persistent identity: range1892276
display id: range1892276
version: 1
top level: BBa_J61002
direction: direction/reverse
start: 1826
end: 2686
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1975766
display id: annotation1975766
version: 1
top level: BBa_J61002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J61002/annotation1975766/range1975766/1

https://synbiohub.org/public/igem/BBa_J61002/annotation1975766/range1975766/1
type: Range
persistent identity: range1975766
display id: range1975766
version: 1
top level: BBa_J61002
direction: direction/forward
start: 9
end: 20
orientation: inline

mRFP
type: SequenceAnnotation
persistent identity: annotation1892273
display id: annotation1892273
version: 1
top level: BBa_J61002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J61002/annotation1892273/range1892273/1

https://synbiohub.org/public/igem/BBa_J61002/annotation1892273/range1892273/1
type: Range
persistent identity: range1892273
display id: range1892273
version: 1
top level: BBa_J61002
direction: direction/forward
start: 27
end: 707
orientation: inline

NotI
type: SequenceAnnotation
persistent identity: annotation1975718
display id: annotation1975718
version: 1
top level: BBa_J61002
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J61002/annotation1975718/range1975718/1

https://synbiohub.org/public/igem/BBa_J61002/annotation1975718/range1975718/1
type: Range
persistent identity: range1975718
display id: range1975718
version: 1
top level: BBa_J61002
direction: direction/forward
start: 878
end: 885
orientation: inline

PstI
type: SequenceAnnotation
persistent identity: annotation1975717
display id: annotation1975717
version: 1
top level: BBa_J61002
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J61002/annotation1975717/range1975717/1

https://synbiohub.org/public/igem/BBa_J61002/annotation1975717/range1975717/1
type: Range
persistent identity: range1975717
display id: range1975717
version: 1
top level: BBa_J61002
direction: direction/forward
start: 885
end: 890
orientation: inline

pBR322 origin
type: SequenceAnnotation
persistent identity: annotation1975714
display id: annotation1975714
version: 1
top level: BBa_J61002
role: feature/dna
role: SO:0000110
location: https://synbiohub.org/public/igem/BBa_J61002/annotation1975714/range1975714/1

https://synbiohub.org/public/igem/BBa_J61002/annotation1975714/range1975714/1
type: Range
persistent identity: range1975714
display id: range1975714
version: 1
top level: BBa_J61002
direction: direction/reverse
start: 1052
end: 1671
orientation: inline

ColE1 origin
type: SequenceAnnotation
persistent identity: annotation1892275
display id: annotation1892275
version: 1
top level: BBa_J61002
role: feature/dna
role: SO:0000110
location: https://synbiohub.org/public/igem/BBa_J61002/annotation1892275/range1892275/1

https://synbiohub.org/public/igem/BBa_J61002/annotation1892275/range1892275/1
type: Range
persistent identity: range1892275
display id: range1892275
version: 1
top level: BBa_J61002
direction: direction/reverse
start: 1049
end: 1731
orientation: inline

beta-lactamase (ampR, bla) promoter
type: SequenceAnnotation
persistent identity: annotation1892272
display id: annotation1892272
version: 1
top level: BBa_J61002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J61002/annotation1892272/range1892272/1

https://synbiohub.org/public/igem/BBa_J61002/annotation1892272/range1892272/1
type: Range
persistent identity: range1892272
display id: range1892272
version: 1
top level: BBa_J61002
direction: direction/reverse
start: 2728
end: 2756
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation1892274
display id: annotation1892274
version: 1
top level: BBa_J61002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J61002/annotation1892274/range1892274/1

https://synbiohub.org/public/igem/BBa_J61002/annotation1892274/range1892274/1
type: Range
persistent identity: range1892274
display id: range1892274
version: 1
top level: BBa_J61002
direction: direction/forward
start: 741
end: 869
orientation: inline

Verification reverse (VR) primer binding site
type: SequenceAnnotation
persistent identity: annotation1975768
display id: annotation1975768
version: 1
top level: BBa_J61002
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J61002/annotation1975768/range1975768/1

https://synbiohub.org/public/igem/BBa_J61002/annotation1975768/range1975768/1
type: Range
persistent identity: range1975768
display id: range1975768
version: 1
top level: BBa_J61002
direction: direction/reverse
start: 948
end: 967
orientation: inline

BioBrick prefix
type: SequenceAnnotation
persistent identity: annotation1975715
display id: annotation1975715
version: 1
top level: BBa_J61002
role: feature/dna
role: SO:0000110
location: https://synbiohub.org/public/igem/BBa_J61002/annotation1975715/range1975715/1

https://synbiohub.org/public/igem/BBa_J61002/annotation1975715/range1975715/1
type: Range
persistent identity: range1975715
display id: range1975715
version: 1
top level: BBa_J61002
direction: direction/forward
start: 2927
end: 2948
orientation: inline

Ambiguity; should be a T
type: SequenceAnnotation
persistent identity: annotation1975921
display id: annotation1975921
version: 1
top level: BBa_J61002
role: feature/mutation
role: SO:0001059
location: https://synbiohub.org/public/igem/BBa_J61002/annotation1975921/range1975921/1

https://synbiohub.org/public/igem/BBa_J61002/annotation1975921/range1975921/1
type: Range
persistent identity: range1975921
display id: range1975921
version: 1
top level: BBa_J61002
direction: direction/forward
start: 2941
end: 2941
orientation: inline

rrnB terminator
type: SequenceAnnotation
persistent identity: annotation1975713
display id: annotation1975713
version: 1
top level: BBa_J61002
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_J61002/annotation1975713/range1975713/1

https://synbiohub.org/public/igem/BBa_J61002/annotation1975713/range1975713/1
type: Range
persistent identity: range1975713
display id: range1975713
version: 1
top level: BBa_J61002
direction: direction/forward
start: 752
end: 795
orientation: inline

BBa_J61003
type: ComponentDefinition
persistent identity: BBa J61003
display id: BBa_J61003
version: 1
was generated by: iGEM to SBOL conversion
description: EN-Ptet-rbs-X-[GFP]-SNP "ORF Expresser"
created: 2006-07-30T11:00:00Z
modified: 2015-08-31T02:02:59Z
mutable provenance: PCR KB001/ca1021R on pSB1A2-J01022 (250 bp, EcoRI/XbaI) sub into pSB1A2-E0040(GFP;DNA-1-5H) (EcoRI/XbaI) Product is pBca1021-E0040 ---- ca1021R Reverse b0034 XbaI AGCCATCTAGAATTTCTCCTCTTTCTCTAG KB001 Forward EcoRI to construct Ptet part-making biobrick plasmid ttctggaattcgcggccgcaactagagccaggcatc
owned by: james
owned by: myers
mutable description: Plasmid for assembly of open reading frame basic parts. A Tet promoter (r0040) and strong ribosome binding site (b0034) lie between the EcoRI and XbaI sites. Insertion of an open reading frame basic part into the XbaI and PstI sites results in constitutive expression but retains basic part utilityin standard assembly.
discontinued: false
dominant: true
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Ptet promoter
sequence annotation: pBR322
sequence annotation: RBS
sequence annotation: ColE1 origin
sequence annotation: BBa_R0040 (Ptet)
sequence annotation: BBa_B0015 (terminator)
sequence annotation: beta-lactamase (ampR) promoter
sequence annotation: BioBrick suffix
sequence annotation: EcoRI
sequence annotation: NotI
sequence annotation: BBa_B0034 RBS
sequence annotation: Verification reverse (VR) primer binding site
sequence annotation: Verification forward (VF2) primer binding site
sequence annotation: XbaI
sequence annotation: rrnB T1 terminator
sequence annotation: beta-lactamase (ampicillin resistance marker)
sequence: https://synbiohub.org/public/igem/BBa_J61003_sequence/1

Ptet promoter
type: SequenceAnnotation
persistent identity: annotation1975757
display id: annotation1975757
version: 1
top level: BBa_J61003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J61003/annotation1975757/range1975757/1

https://synbiohub.org/public/igem/BBa_J61003/annotation1975757/range1975757/1
type: Range
persistent identity: range1975757
display id: range1975757
version: 1
top level: BBa_J61003
direction: direction/forward
start: 2217
end: 2270
orientation: inline

pBR322
type: SequenceAnnotation
persistent identity: annotation1975748
display id: annotation1975748
version: 1
top level: BBa_J61003
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J61003/annotation1975748/range1975748/1

https://synbiohub.org/public/igem/BBa_J61003/annotation1975748/range1975748/1
type: Range
persistent identity: range1975748
display id: range1975748
version: 1
top level: BBa_J61003
direction: direction/reverse
start: 183
end: 802
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1975756
display id: annotation1975756
version: 1
top level: BBa_J61003
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J61003/annotation1975756/range1975756/1

https://synbiohub.org/public/igem/BBa_J61003/annotation1975756/range1975756/1
type: Range
persistent identity: range1975756
display id: range1975756
version: 1
top level: BBa_J61003
direction: direction/forward
start: 2279
end: 2290
orientation: inline

ColE1 origin
type: SequenceAnnotation
persistent identity: annotation1892280
display id: annotation1892280
version: 1
top level: BBa_J61003
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J61003/annotation1892280/range1892280/1

https://synbiohub.org/public/igem/BBa_J61003/annotation1892280/range1892280/1
type: Range
persistent identity: range1892280
display id: range1892280
version: 1
top level: BBa_J61003
direction: direction/forward
start: 180
end: 862
orientation: inline

BBa_R0040 (Ptet)
type: SequenceAnnotation
persistent identity: annotation1892278
display id: annotation1892278
version: 1
top level: BBa_J61003
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J61003/annotation1892278/range1892278/1

https://synbiohub.org/public/igem/BBa_J61003/annotation1892278/range1892278/1
type: Range
persistent identity: range1892278
display id: range1892278
version: 1
top level: BBa_J61003
direction: direction/forward
start: 2217
end: 2270
orientation: inline

BBa_B0015 (terminator)
type: SequenceAnnotation
persistent identity: annotation1892277
display id: annotation1892277
version: 1
top level: BBa_J61003
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J61003/annotation1892277/range1892277/1

https://synbiohub.org/public/igem/BBa_J61003/annotation1892277/range1892277/1
type: Range
persistent identity: range1892277
display id: range1892277
version: 1
top level: BBa_J61003
direction: direction/forward
start: 2080
end: 2208
orientation: inline

beta-lactamase (ampR) promoter
type: SequenceAnnotation
persistent identity: annotation1892279
display id: annotation1892279
version: 1
top level: BBa_J61003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J61003/annotation1892279/range1892279/1

https://synbiohub.org/public/igem/BBa_J61003/annotation1892279/range1892279/1
type: Range
persistent identity: range1892279
display id: range1892279
version: 1
top level: BBa_J61003
direction: direction/reverse
start: 1859
end: 1887
orientation: inline

BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation1975749
display id: annotation1975749
version: 1
top level: BBa_J61003
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J61003/annotation1975749/range1975749/1

https://synbiohub.org/public/igem/BBa_J61003/annotation1975749/range1975749/1
type: Range
persistent identity: range1975749
display id: range1975749
version: 1
top level: BBa_J61003
direction: direction/forward
start: 1
end: 21
orientation: inline

EcoRI
type: SequenceAnnotation
persistent identity: annotation1975750
display id: annotation1975750
version: 1
top level: BBa_J61003
role: feature/dna
role: SO:0000110
location: https://synbiohub.org/public/igem/BBa_J61003/annotation1975750/range1975750/1

https://synbiohub.org/public/igem/BBa_J61003/annotation1975750/range1975750/1
type: Range
persistent identity: range1975750
display id: range1975750
version: 1
top level: BBa_J61003
direction: direction/forward
start: 2058
end: 2063
orientation: inline

NotI
type: SequenceAnnotation
persistent identity: annotation1975751
display id: annotation1975751
version: 1
top level: BBa_J61003
role: feature/dna
role: SO:0000110
location: https://synbiohub.org/public/igem/BBa_J61003/annotation1975751/range1975751/1

https://synbiohub.org/public/igem/BBa_J61003/annotation1975751/range1975751/1
type: Range
persistent identity: range1975751
display id: range1975751
version: 1
top level: BBa_J61003
direction: direction/forward
start: 2064
end: 2071
orientation: inline

BBa_B0034 RBS
type: SequenceAnnotation
persistent identity: annotation1975755
display id: annotation1975755
version: 1
top level: BBa_J61003
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J61003/annotation1975755/range1975755/1

https://synbiohub.org/public/igem/BBa_J61003/annotation1975755/range1975755/1
type: Range
persistent identity: range1975755
display id: range1975755
version: 1
top level: BBa_J61003
direction: direction/forward
start: 2279
end: 2290
orientation: inline

Verification reverse (VR) primer binding site
type: SequenceAnnotation
persistent identity: annotation1975754
display id: annotation1975754
version: 1
top level: BBa_J61003
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J61003/annotation1975754/range1975754/1

https://synbiohub.org/public/igem/BBa_J61003/annotation1975754/range1975754/1
type: Range
persistent identity: range1975754
display id: range1975754
version: 1
top level: BBa_J61003
direction: direction/reverse
start: 79
end: 98
orientation: inline

Verification forward (VF2) primer binding site
type: SequenceAnnotation
persistent identity: annotation1975753
display id: annotation1975753
version: 1
top level: BBa_J61003
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J61003/annotation1975753/range1975753/1

https://synbiohub.org/public/igem/BBa_J61003/annotation1975753/range1975753/1
type: Range
persistent identity: range1975753
display id: range1975753
version: 1
top level: BBa_J61003
direction: direction/forward
start: 1940
end: 1959
orientation: inline

XbaI
type: SequenceAnnotation
persistent identity: annotation1975752
display id: annotation1975752
version: 1
top level: BBa_J61003
role: feature/dna
role: SO:0000110
location: https://synbiohub.org/public/igem/BBa_J61003/annotation1975752/range1975752/1

https://synbiohub.org/public/igem/BBa_J61003/annotation1975752/range1975752/1
type: Range
persistent identity: range1975752
display id: range1975752
version: 1
top level: BBa_J61003
direction: direction/forward
start: 2292
end: 2297
orientation: inline

rrnB T1 terminator
type: SequenceAnnotation
persistent identity: annotation1975747
display id: annotation1975747
version: 1
top level: BBa_J61003
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_J61003/annotation1975747/range1975747/1

https://synbiohub.org/public/igem/BBa_J61003/annotation1975747/range1975747/1
type: Range
persistent identity: range1975747
display id: range1975747
version: 1
top level: BBa_J61003
direction: direction/forward
start: 2091
end: 2134
orientation: inline

beta-lactamase (ampicillin resistance marker)
type: SequenceAnnotation
persistent identity: annotation1892281
display id: annotation1892281
version: 1
top level: BBa_J61003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J61003/annotation1892281/range1892281/1

https://synbiohub.org/public/igem/BBa_J61003/annotation1892281/range1892281/1
type: Range
persistent identity: range1892281
display id: range1892281
version: 1
top level: BBa_J61003
direction: direction/reverse
start: 957
end: 1817
orientation: inline

pSBBs3C
type: ComponentDefinition
persistent identity: BBa K823025
display id: BBa_K823025
version: 1
was generated by: iGEM to SBOL conversion
description: pSBBs3C-luxABCDE (lux reporter vector for B.subtilis)
created: 2012-08-20T11:00:00Z
modified: 2015-05-08T01:13:30Z
mutable provenance: This vector is an igem compatible version of the pAH328 Bacillus reporter vecotr.
owned by: james
owned by: myers
mutable description: This part is a reporter vector for Bacillus subtilis. It integrates at the sacA locus of B.subtilis genome. It has a ampicillin resistance for cloning in E.coli and Chloramphenicol resistance for selection in B. subtilis. The multiple cloning site has a rfp for selection and is followed by the luxABCDE gene cluster for luminescence reporter assays. This BioBrick is part of the LMU-Munich 2012 igem project Bacillus BioBrickBOX http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 0
owner id: 11555
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: We removed any forbidden restriction sites by site-directed mutagenesis PCR. The clonging site also includes a rfp for selection for cloning in E.coli.
star: false
creator: Jara Radeck
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: luxE
sequence annotation: luxD
sequence annotation: bla
sequence annotation: cat
sequence annotation: luxA
sequence annotation: luxB
sequence annotation: sacA
sequence annotation: luxC
sequence: https://synbiohub.org/public/igem/BBa_K823025_sequence/1

luxE
type: SequenceAnnotation
persistent identity: annotation2180251
display id: annotation2180251
version: 1
top level: pSBBs3C
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823025/annotation2180251/range2180251/1

https://synbiohub.org/public/igem/BBa_K823025/annotation2180251/range2180251/1
type: Range
persistent identity: range2180251
display id: range2180251
version: 1
top level: pSBBs3C
direction: direction/forward
start: 4583
end: 5695
orientation: inline

luxD
type: SequenceAnnotation
persistent identity: annotation2180250
display id: annotation2180250
version: 1
top level: pSBBs3C
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823025/annotation2180250/range2180250/1

https://synbiohub.org/public/igem/BBa_K823025/annotation2180250/range2180250/1
type: Range
persistent identity: range2180250
display id: range2180250
version: 1
top level: pSBBs3C
direction: direction/forward
start: 3635
end: 4558
orientation: inline

bla
type: SequenceAnnotation
persistent identity: annotation2180253
display id: annotation2180253
version: 1
top level: pSBBs3C
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823025/annotation2180253/range2180253/1

https://synbiohub.org/public/igem/BBa_K823025/annotation2180253/range2180253/1
type: Range
persistent identity: range2180253
display id: range2180253
version: 1
top level: pSBBs3C
direction: direction/forward
start: 6849
end: 7721
orientation: inline

cat
type: SequenceAnnotation
persistent identity: annotation2180255
display id: annotation2180255
version: 1
top level: pSBBs3C
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823025/annotation2180255/range2180255/1

https://synbiohub.org/public/igem/BBa_K823025/annotation2180255/range2180255/1
type: Range
persistent identity: range2180255
display id: range2180255
version: 1
top level: pSBBs3C
direction: direction/reverse
start: 9661
end: 10323
orientation: inline

luxA
type: SequenceAnnotation
persistent identity: annotation2180247
display id: annotation2180247
version: 1
top level: pSBBs3C
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823025/annotation2180247/range2180247/1

https://synbiohub.org/public/igem/BBa_K823025/annotation2180247/range2180247/1
type: Range
persistent identity: range2180247
display id: range2180247
version: 1
top level: pSBBs3C
direction: direction/forward
start: 73
end: 1155
orientation: inline

luxB
type: SequenceAnnotation
persistent identity: annotation2180248
display id: annotation2180248
version: 1
top level: pSBBs3C
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823025/annotation2180248/range2180248/1

https://synbiohub.org/public/igem/BBa_K823025/annotation2180248/range2180248/1
type: Range
persistent identity: range2180248
display id: range2180248
version: 1
top level: pSBBs3C
direction: direction/forward
start: 1172
end: 2155
orientation: inline

sacA
type: SequenceAnnotation
persistent identity: annotation2180254
display id: annotation2180254
version: 1
top level: pSBBs3C
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823025/annotation2180254/range2180254/1

https://synbiohub.org/public/igem/BBa_K823025/annotation2180254/range2180254/1
type: Range
persistent identity: range2180254
display id: range2180254
version: 1
top level: pSBBs3C
direction: direction/forward
start: 8712
end: 9503
orientation: inline

sacA
type: SequenceAnnotation
persistent identity: annotation2180252
display id: annotation2180252
version: 1
top level: pSBBs3C
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823025/annotation2180252/range2180252/1

https://synbiohub.org/public/igem/BBa_K823025/annotation2180252/range2180252/1
type: Range
persistent identity: range2180252
display id: range2180252
version: 1
top level: pSBBs3C
direction: direction/forward
start: 5676
end: 6515
orientation: inline

luxC
type: SequenceAnnotation
persistent identity: annotation2180249
display id: annotation2180249
version: 1
top level: pSBBs3C
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823025/annotation2180249/range2180249/1

https://synbiohub.org/public/igem/BBa_K823025/annotation2180249/range2180249/1
type: Range
persistent identity: range2180249
display id: range2180249
version: 1
top level: pSBBs3C
direction: direction/forward
start: 2179
end: 3621
orientation: inline

BBa_J23019
type: ComponentDefinition
persistent identity: BBa J23019
display id: BBa_J23019
version: 1
was generated by: iGEM to SBOL conversion
description: EN-Ptet-rbs-X-[RFP]-SNP "ORF Expresser"
created: 2006-08-02T11:00:00Z
modified: 2015-08-31T04:08:39Z
mutable provenance: J23018 pAC997
owned by: james
owned by: myers
mutable description: Variant of pBca1021 (J61003) with GFP inserted. Part J23019 is the same as J23018 but in pSB3C6.
discontinued: false
dominant: false
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: chloramphenicol acetyl transferase (CmR resistance marker)
sequence annotation: BioBrick suffix
sequence annotation: NotI
sequence annotation: p15A origin
sequence annotation: BBa_B0015 terminator
sequence annotation: Ptet
sequence annotation: EcoRI
sequence annotation: XbaI
sequence annotation: RBS
sequence annotation: BBa_R0040 (Ptet)
sequence annotation: BBa_B0034 RBS
sequence annotation: rrnB T1 terminator
sequence: https://synbiohub.org/public/igem/BBa_J23019_sequence/1

chloramphenicol acetyl transferase (CmR resistance marker)
type: SequenceAnnotation
persistent identity: annotation1975742
display id: annotation1975742
version: 1
top level: BBa_J23019
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J23019/annotation1975742/range1975742/1

https://synbiohub.org/public/igem/BBa_J23019/annotation1975742/range1975742/1
type: Range
persistent identity: range1975742
display id: range1975742
version: 1
top level: BBa_J23019
direction: direction/forward
start: 1232
end: 1891
orientation: inline

BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation1893440
display id: annotation1893440
version: 1
top level: BBa_J23019
role: feature/dna
role: SO:0000110
location: https://synbiohub.org/public/igem/BBa_J23019/annotation1893440/range1893440/1

https://synbiohub.org/public/igem/BBa_J23019/annotation1893440/range1893440/1
type: Range
persistent identity: range1893440
display id: range1893440
version: 1
top level: BBa_J23019
direction: direction/forward
start: 1
end: 21
orientation: inline

NotI
type: SequenceAnnotation
persistent identity: annotation1975745
display id: annotation1975745
version: 1
top level: BBa_J23019
role: feature/dna
role: SO:0000110
location: https://synbiohub.org/public/igem/BBa_J23019/annotation1975745/range1975745/1

https://synbiohub.org/public/igem/BBa_J23019/annotation1975745/range1975745/1
type: Range
persistent identity: range1975745
display id: range1975745
version: 1
top level: BBa_J23019
direction: direction/forward
start: 2050
end: 2057
orientation: inline

p15A origin
type: SequenceAnnotation
persistent identity: annotation1975741
display id: annotation1975741
version: 1
top level: BBa_J23019
role: feature/dna
role: SO:0000110
location: https://synbiohub.org/public/igem/BBa_J23019/annotation1975741/range1975741/1

https://synbiohub.org/public/igem/BBa_J23019/annotation1975741/range1975741/1
type: Range
persistent identity: range1975741
display id: range1975741
version: 1
top level: BBa_J23019
direction: direction/reverse
start: 22
end: 870
orientation: inline

BBa_B0015 terminator
type: SequenceAnnotation
persistent identity: annotation1893438
display id: annotation1893438
version: 1
top level: BBa_J23019
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J23019/annotation1893438/range1893438/1

https://synbiohub.org/public/igem/BBa_J23019/annotation1893438/range1893438/1
type: Range
persistent identity: range1893438
display id: range1893438
version: 1
top level: BBa_J23019
direction: direction/forward
start: 2066
end: 2194
orientation: inline

Ptet
type: SequenceAnnotation
persistent identity: annotation1975762
display id: annotation1975762
version: 1
top level: BBa_J23019
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J23019/annotation1975762/range1975762/1

https://synbiohub.org/public/igem/BBa_J23019/annotation1975762/range1975762/1
type: Range
persistent identity: range1975762
display id: range1975762
version: 1
top level: BBa_J23019
direction: direction/forward
start: 2203
end: 2256
orientation: inline

EcoRI
type: SequenceAnnotation
persistent identity: annotation1975744
display id: annotation1975744
version: 1
top level: BBa_J23019
role: feature/dna
role: SO:0000110
location: https://synbiohub.org/public/igem/BBa_J23019/annotation1975744/range1975744/1

https://synbiohub.org/public/igem/BBa_J23019/annotation1975744/range1975744/1
type: Range
persistent identity: range1975744
display id: range1975744
version: 1
top level: BBa_J23019
direction: direction/forward
start: 2044
end: 2049
orientation: inline

XbaI
type: SequenceAnnotation
persistent identity: annotation1975746
display id: annotation1975746
version: 1
top level: BBa_J23019
role: feature/dna
role: SO:0000110
location: https://synbiohub.org/public/igem/BBa_J23019/annotation1975746/range1975746/1

https://synbiohub.org/public/igem/BBa_J23019/annotation1975746/range1975746/1
type: Range
persistent identity: range1975746
display id: range1975746
version: 1
top level: BBa_J23019
direction: direction/forward
start: 2278
end: 2283
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1975758
display id: annotation1975758
version: 1
top level: BBa_J23019
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J23019/annotation1975758/range1975758/1

https://synbiohub.org/public/igem/BBa_J23019/annotation1975758/range1975758/1
type: Range
persistent identity: range1975758
display id: range1975758
version: 1
top level: BBa_J23019
direction: direction/forward
start: 2265
end: 2276
orientation: inline

BBa_R0040 (Ptet)
type: SequenceAnnotation
persistent identity: annotation1893439
display id: annotation1893439
version: 1
top level: BBa_J23019
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J23019/annotation1893439/range1893439/1

https://synbiohub.org/public/igem/BBa_J23019/annotation1893439/range1893439/1
type: Range
persistent identity: range1893439
display id: range1893439
version: 1
top level: BBa_J23019
direction: direction/forward
start: 2203
end: 2256
orientation: inline

BBa_B0034 RBS
type: SequenceAnnotation
persistent identity: annotation1975759
display id: annotation1975759
version: 1
top level: BBa_J23019
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J23019/annotation1975759/range1975759/1

https://synbiohub.org/public/igem/BBa_J23019/annotation1975759/range1975759/1
type: Range
persistent identity: range1975759
display id: range1975759
version: 1
top level: BBa_J23019
direction: direction/forward
start: 2265
end: 2276
orientation: inline

rrnB T1 terminator
type: SequenceAnnotation
persistent identity: annotation1975743
display id: annotation1975743
version: 1
top level: BBa_J23019
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_J23019/annotation1975743/range1975743/1

https://synbiohub.org/public/igem/BBa_J23019/annotation1975743/range1975743/1
type: Range
persistent identity: range1975743
display id: range1975743
version: 1
top level: BBa_J23019
direction: direction/forward
start: 2077
end: 2120
orientation: inline

BBa_I732950
type: ComponentDefinition
persistent identity: BBa I732950
display id: BBa_I732950
version: 1
was generated by: iGEM to SBOL conversion
description: P_template1 + lacZ
created: 2007-10-25T11:00:00Z
modified: 2015-08-31T04:08:00Z
mutable provenance: N/A
owned by: james
owned by: myers
mutable description: Intermediate in the construction of P_template1
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: N/A
star: false
creator: Zhan Jian
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: End of terminator
sequence annotation: Verification reverse (VR) primer binding site
sequence annotation: beta-lactamase ampicillin resistance marker
sequence annotation: Stem loop
sequence annotation: kanamycin resistance marker
sequence annotation: beta-lactamase (ampR) promoter
sequence annotation: Deletion of AC
sequence annotation: Verification forward (VF2) primer binding site
sequence annotation: E. coli his operon terminator
sequence annotation: Ptemplate1?
sequence annotation: pBR322 origin
sequence annotation: EcoRI
sequence annotation: BioBrick suffix
sequence annotation: XbaI
sequence: https://synbiohub.org/public/igem/BBa_I732950_sequence/1

End of terminator
type: SequenceAnnotation
persistent identity: annotation1975783
display id: annotation1975783
version: 1
top level: BBa_I732950
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I732950/annotation1975783/range1975783/1

https://synbiohub.org/public/igem/BBa_I732950/annotation1975783/range1975783/1
type: Range
persistent identity: range1975783
display id: range1975783
version: 1
top level: BBa_I732950
direction: direction/reverse
start: 3126
end: 3129
orientation: inline

Verification reverse (VR) primer binding site
type: SequenceAnnotation
persistent identity: annotation1975778
display id: annotation1975778
version: 1
top level: BBa_I732950
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_I732950/annotation1975778/range1975778/1

https://synbiohub.org/public/igem/BBa_I732950/annotation1975778/range1975778/1
type: Range
persistent identity: range1975778
display id: range1975778
version: 1
top level: BBa_I732950
direction: direction/reverse
start: 157
end: 176
orientation: inline

beta-lactamase ampicillin resistance marker
type: SequenceAnnotation
persistent identity: annotation1975772
display id: annotation1975772
version: 1
top level: BBa_I732950
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I732950/annotation1975772/range1975772/1

https://synbiohub.org/public/igem/BBa_I732950/annotation1975772/range1975772/1
type: Range
persistent identity: range1975772
display id: range1975772
version: 1
top level: BBa_I732950
direction: direction/reverse
start: 2067
end: 2927
orientation: inline

Stem loop
type: SequenceAnnotation
persistent identity: annotation1975782
display id: annotation1975782
version: 1
top level: BBa_I732950
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I732950/annotation1975782/range1975782/1

https://synbiohub.org/public/igem/BBa_I732950/annotation1975782/range1975782/1
type: Range
persistent identity: range1975782
display id: range1975782
version: 1
top level: BBa_I732950
direction: direction/reverse
start: 3139
end: 3158
orientation: inline

kanamycin resistance marker
type: SequenceAnnotation
persistent identity: annotation1975771
display id: annotation1975771
version: 1
top level: BBa_I732950
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I732950/annotation1975771/range1975771/1

https://synbiohub.org/public/igem/BBa_I732950/annotation1975771/range1975771/1
type: Range
persistent identity: range1975771
display id: range1975771
version: 1
top level: BBa_I732950
direction: direction/reverse
start: 1097
end: 1912
orientation: inline

beta-lactamase (ampR) promoter
type: SequenceAnnotation
persistent identity: annotation1975773
display id: annotation1975773
version: 1
top level: BBa_I732950
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I732950/annotation1975773/range1975773/1

https://synbiohub.org/public/igem/BBa_I732950/annotation1975773/range1975773/1
type: Range
persistent identity: range1975773
display id: range1975773
version: 1
top level: BBa_I732950
direction: direction/reverse
start: 2969
end: 2997
orientation: inline

Deletion of AC
type: SequenceAnnotation
persistent identity: annotation1975780
display id: annotation1975780
version: 1
top level: BBa_I732950
role: feature/mutation
role: SO:0001059
location: https://synbiohub.org/public/igem/BBa_I732950/annotation1975780/range1975780/1

https://synbiohub.org/public/igem/BBa_I732950/annotation1975780/range1975780/1
type: Range
persistent identity: range1975780
display id: range1975780
version: 1
top level: BBa_I732950
direction: direction/forward
start: 34
end: 35
orientation: inline

Verification forward (VF2) primer binding site
type: SequenceAnnotation
persistent identity: annotation1975777
display id: annotation1975777
version: 1
top level: BBa_I732950
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_I732950/annotation1975777/range1975777/1

https://synbiohub.org/public/igem/BBa_I732950/annotation1975777/range1975777/1
type: Range
persistent identity: range1975777
display id: range1975777
version: 1
top level: BBa_I732950
direction: direction/forward
start: 3050
end: 3069
orientation: inline

E. coli his operon terminator
type: SequenceAnnotation
persistent identity: annotation1975779
display id: annotation1975779
version: 1
top level: BBa_I732950
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I732950/annotation1975779/range1975779/1

https://synbiohub.org/public/igem/BBa_I732950/annotation1975779/range1975779/1
type: Range
persistent identity: range1975779
display id: range1975779
version: 1
top level: BBa_I732950
direction: direction/forward
start: 22
end: 93
orientation: inline

Stem loop
type: SequenceAnnotation
persistent identity: annotation1975781
display id: annotation1975781
version: 1
top level: BBa_I732950
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I732950/annotation1975781/range1975781/1

https://synbiohub.org/public/igem/BBa_I732950/annotation1975781/range1975781/1
type: Range
persistent identity: range1975781
display id: range1975781
version: 1
top level: BBa_I732950
direction: direction/forward
start: 30
end: 64
orientation: inline

Ptemplate1?
type: SequenceAnnotation
persistent identity: annotation1975784
display id: annotation1975784
version: 1
top level: BBa_I732950
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I732950/annotation1975784/range1975784/1

https://synbiohub.org/public/igem/BBa_I732950/annotation1975784/range1975784/1
type: Range
persistent identity: range1975784
display id: range1975784
version: 1
top level: BBa_I732950
direction: direction/forward
start: 3174
end: 3332
orientation: inline

pBR322 origin
type: SequenceAnnotation
persistent identity: annotation1975770
display id: annotation1975770
version: 1
top level: BBa_I732950
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_I732950/annotation1975770/range1975770/1

https://synbiohub.org/public/igem/BBa_I732950/annotation1975770/range1975770/1
type: Range
persistent identity: range1975770
display id: range1975770
version: 1
top level: BBa_I732950
direction: direction/reverse
start: 261
end: 880
orientation: inline

EcoRI
type: SequenceAnnotation
persistent identity: annotation1975775
display id: annotation1975775
version: 1
top level: BBa_I732950
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_I732950/annotation1975775/range1975775/1

https://synbiohub.org/public/igem/BBa_I732950/annotation1975775/range1975775/1
type: Range
persistent identity: range1975775
display id: range1975775
version: 1
top level: BBa_I732950
direction: direction/forward
start: 3168
end: 3173
orientation: inline

BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation1975774
display id: annotation1975774
version: 1
top level: BBa_I732950
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_I732950/annotation1975774/range1975774/1

https://synbiohub.org/public/igem/BBa_I732950/annotation1975774/range1975774/1
type: Range
persistent identity: range1975774
display id: range1975774
version: 1
top level: BBa_I732950
direction: direction/forward
start: 1
end: 21
orientation: inline

XbaI
type: SequenceAnnotation
persistent identity: annotation1975776
display id: annotation1975776
version: 1
top level: BBa_I732950
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_I732950/annotation1975776/range1975776/1

https://synbiohub.org/public/igem/BBa_I732950/annotation1975776/range1975776/1
type: Range
persistent identity: range1975776
display id: range1975776
version: 1
top level: BBa_I732950
direction: direction/forward
start: 3333
end: 3338
orientation: inline

https://synbiohub.org/public/igem/BBa_I732950_sequence/1
type: Sequence
persistent identity: BBa I732950 sequence
display id: BBa_I732950_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagtagcggccgctgcagtccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagctcgagtcccgtcaagtcagcgtaatgctctgccagtgttacaaccaattaaccaattctgattagaaaaactcatcgagcatcaaatgaaactgcaatttattcatatcaggattatcaataccatatttttgaaaaagccgtttctgtaatgaaggagaaaactcaccgaggcagttccataggatggcaagatcctggtatcggtctgcgattccgactcgtccaacatcaatacaacctattaatttcccctcgtcaaaaataaggttatcaagtgagaaatcaccatgagtgacgactgaatccggtgagaatggcaaaagcttatgcatttctttccagacttgttcaacaggccagccattacgctcgtcatcaaaatcactcgcatcaaccaaaccgttattcattcgtgattgcgcctgagcgagacgaaatacgcgatcgctgttaaaaggacaattacaaacaggaatcgaatgcaaccggcgcaggaacactgccagcgcatcaacaatattttcacctgaatcaggatattcttctaatacctggaatgctgttttcccggggatcgcagtggtgagtaaccatgcatcatcaggagtacggataaaatgcttgatggtcggaagaggcataaattccgtcagccagtttagtctgaccatctcatctgtaacatcattggcaacgctacctttgccatgtttcagaaacaactctggcgcatcgggcttcccatacaatcgatagattgtcgcacctgattgcccgacattatcgcgagcccatttatacccatataaatcagcatccatgttggaatttaatcgcggcctggagcaagacgtttcccgttgaatatggctcataacaccccttgtattactgtttatgtaagcagacagttttattgttcatgatgatatatttttatcttgtgcaatgtaacatcagaga…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J61003_sequence/1
type: Sequence
persistent identity: BBa J61003 sequence
display id: BBa_J61003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagtagcggccgctgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtat…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K823021_sequence/1
type: Sequence
persistent identity: BBa K823021 sequence
display id: BBa_K823021_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagtagcggccgctgcagggatccacagtacataaaaaaggagacatgacgatggtcgttttacaacgtgactgggtcgaccgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtgcgatcttcctgaggccgatactgtcgtcgtcccctcaaactggcagatgcacggttacgatgcgcccatctacaccaacgtaacctatcccattacggtcaatccgccgtttgttcccacggagaatccgacgggttgttactcgctcacatttaatgttgatgaaagctggctacaggaaggccagacgcgaattatttttgatggcgttaactcggcgtttcatctgtggtgcaacgggcgctgggtcggttacggccaggacagtcgtttgccgtctgaatttgacctgagcgcatttttacgcgccggagaaaaccgcctcgcggtgatggtgctgcgttggagtgacggcagttatctggaagatcaggatatgtggcggatgagcggcattttccgtgacgtctcgttgctgcataaaccgactacacaaatcagcgatttccatgttgccactcgctttaatgatgatttcagccgcgctgtactggaggctgaagttcagatgtgcggcgagttgcgtgactacctacgggtaacagtttctttatggcagggtgaaacgcaggtcgccagcggcaccgcgcctttcggcggtgaaattatcgatgagcgtggtggttatgccgatcgcgtcacactacgtctgaacgtcgaaaacccgaaactgtggagcgccgaaatcccgaatctctatcgtgcggtggttgaactgcacaccgccgacggcacgctgattgaagcagaagcctgcgatgtcggtttccgcgaggtgcggattgaaaatggtctgctgctgctgaacggcaagccgttgctgattcgaggcgttaaccgtcacgagcatcatcctctgcatggtcaggtcatggatgagcagacgatggtgcaggatatcctgctgatgaagcagaacaactttaacgccgtgcgctgttcgcattatccgaaccatccgctgtggtacacgctgtgcgaccgctacggcctgtatgtggtggatgaagccaatattgaaacccacggcatggtgccaatgaatcgtctgaccgatgatccgcgctggctaccggcgatgagcgaacgcgtaacgcgaatggtgcagcgcgatcgtaatcacccgagtgtgatcatctggtcgctggggaatgaatcaggccacggcgctaatcacgacgcgctgtatcgctggatcaaatctgtcgatccttcccgcccggtgcagtatgaaggcggcggagccgacaccacggccaccgatattatttgcccgatgtacgcgcgcgtggatgaagaccagcccttcccggctgtgccgaaatggtccatcaaaaaatggctttcgctacctggagagacgcgcccgctgatcctttgcgaatacgcccacgcgatgggtaacagtcttggcggtttcgctaaatactggcaggcgtttcgtcagtatccccgtttacagggcggcttcgtctgggactgggtggatcagtcgctgattaaatatgatgaaaacggcaacccgtggtcggcttacggcggtgattttggcgatacgccgaacgatcgccagttctgtatgaacggtctggtctttgccgaccgcacgccgcatccagcgctgacggaagcaaaacaccagcagcagtttttccagttccgtttatccgggcaaaccatcgaagtgaccagcgaatacctgttccgtcatagcgataacgagctcctgcactg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23006_sequence/1
type: Sequence
persistent identity: BBa J23006 sequence
display id: BBa_J23006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagtagcggccgctgcagtccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J61002_sequence/1
type: Sequence
persistent identity: BBa J61002 sequence
display id: BBa_J61002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagtgaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaagcggccgctgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagca…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K678070_sequence/1
type: Sequence
persistent identity: BBa K678070 sequence
display id: BBa_K678070_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: accaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagcgctgcgatgataccgcgagaaccacgctcaccggctccggatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccatcgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatattcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggtcagtgttacaaccaattaaccaattctgaacattatcgcgagcccatttatacctgaatatggctcataacaccccttgtttgcctggcggcagtagcgcggtggtcccacctgaccccatgccgaactcagaagtgaaacgccgtagcgccgatggtagtgtggggactccccatgcgagagtagggaactgccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgcccgggctaattatggggtgtcgcccttattcgactctatagtgaagttcctattctctagaaagtataggaacttctgaagtggggatttaaatgcggccgcgctgagggtttaatcgacgccagcagtgtcacaatcgaccacgacccagatcggttgtactttttgcgcgaagactctgacacagcaaccagggaatttattgatcttactgaggatgagacgacgcatgatggcgaatacataaccgacgagtgtttcgaggtactgcgcgaggactggcttgcttccgcgtgtgcttccagtcctctctcgccggagcttccggaagggcagctgctagaagaggtctgtgatgctgacggaattgtctacaagccgggcaattccgtcgagctccacgatggcgcattcttccgaatttgctctatacggcaagcattgaaaggcaatataatattgaccggccgccggttgctcaaattcaaagaccacccagacaaataccttccccaatggcgcaatgagctgatttgggttgccgatgaaactgcggagattcctctctggttcgttcgacgttttgtctcagtacacttcagcaacgtttgtcccattggtcaagactgtcagaagaataataatcctgatggcctattctgccgcttgaagagggttattcagaaagagactacttcaattgaatacctgacattcgaggaagccgatgccgggttccgcgcgccctcggcaagcttgcgccatggttggcggggggaaaccgctcctttcggcagca…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23019_sequence/1
type: Sequence
persistent identity: BBa J23019 sequence
display id: BBa_J23019_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagtagcggccgctgcagctagaaatattttatctgattaataagatgatcttcttgagatcgttttggtctgcgcgtaatctcttgctctgaaaacgaaaaaaccgccttgcagggcggtttttcgaaggttctctgagctaccaactctttgaaccgaggtaactggcttggaggagcgcagtcaccaaaacttgtcctttcagtttagccttaaccggcgcatgacttcaagactaactcctctaaatcaattaccagtggctgctgccagtggtgcttttgcatgtctttccgggttggactcaagacgatagttaccggataaggcgcagcggtcggactgaacggggggttcgtgcatacagtccagcttggagcgaactgcctacccggaactgagtgtcaggcgtggaatgagacaaacgcggccataacagcggaatgacaccggtaaaccgaaaggcaggaacaggagagcgcacgagggagccgccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccaccactgatttgagcgtcagatttcgtgatgcttgtcaggggggcggagcctatggaaaaacggctttgccgcggccctctcacttccctgttaagtatcttcctggcatcttccaggaaatctccgccccgttcgtaagccatttccgctcgccgcagtcgaacgaccgagcgtagcgagtcagtgagcgaggaagcggaatatatcctgtatcacatattctgctgacgcaccggtgcagccttttttctcctgccacatgaagcacttcactgacaccctcatcagtgccaacatagtaagccagtatacactccgctagcgctgatgtccggcggtgcttttgccgttacgcaccaccccgtcagtagctgaacaggagggacagctgatagaaacagaagccactggagcacctcaaaaacaccatcatacactaaatcagtaagttggcagcatcacccgacgcactttgcgccgaataaatacctgtgacggaagatcacttcgcagaataaataaatcctggtgtccctgttgataccgggaagccctgggccaacttttggcgaaaatgagacgttgatcggcacgtaagaggttccaactttcaccataatgaaataagatcactaccgggcgtattttttgagttatcgagattttcaggagctaaggaagctaaaatggagaaaaaaatcactggatataccaccgttgatatatcccaatggcatcgtaaagaacattttgaggcatttcagtcagttgctcaatgtacctataaccagaccgttcagctggatattacggcctttttaaagaccgtaaagaaaaataagcacaagttttatccggcctttattcacattcttgcccgcctgatgaatgctcatccggagttccgtatggcaatgaaagacggtgagctggtgatatgggatagtgttcacccttgttacaccgttttccatgagcaaactgaaacgttttcatcgctctggagtgaataccacgacgatttccggcagtttctacacatatattcgcaagatgtggcgtgttacggtgaaaacctggcctatttccctaaagggtttattgagaatatgtttttcgtctcagccaatccctgggtgagtttcaccagttttgatttaaacgtggccaatatggacaacttcttcgcccccgttttcacgatgggcaaatattatacgcaaggcgacaaggtgctgatgccgctggcgattcaggttcatcatgccgtctgtgatggcttccatgtcggcagaatgcttaatgaattacaacagtactgcgatgagtggcagggcggggcgtaatttttttaaggcagttattggtgcccttaaacgcctggtgctacgcctgaataagtgataataagcggatgaatggcagaaattcgaaagcaaattcgacccggtcgtc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I732952_sequence/1
type: Sequence
persistent identity: BBa I732952 sequence
display id: BBa_I732952_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagtagcggccgctgcagtccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K823025_sequence/1
type: Sequence
persistent identity: BBa K823025 sequence
display id: BBa_K823025_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagtagcggccgctgcagtccggcaaaaaagggcaaggtgtcctagtaaggtcgacaggaggactctctatgaaatttggaaactttttgcttacataccaacctccccaattttctcaaacagaggtaatgaaacgtttggttaaattaggtcgcatctctgaggagtgtggttttgataccgtatggttactggagcatcatttcacggagtttggtttgcttggtaacccttatgtcgctgctgcatatttacttggcgcgactaaaaaattgaatgtaggaactgccgctattgttcttcccacagcccatccagtacgccaacttgaagatgtgaatttattggatcaaatgtcaaaaggacgatttcggtttggtatttgccgagggctttacaacaaggactttcgcgtattcggcacagatatgaataacagtcgcgccttagcggaatgctggtacgggctgataaagaatggcatgacagagggatatatggaagctgataatgaacatatcaagttccataaggtaaaagtaaaccccgcggcgtatagcagaggtggcgcaccggtttatgtggtggctgaatcagcttcgacgactgagtgggctgctcaatttggcctaccgatgatattaagttggattataaatactaacgaaaagaaagcacaacttgagctttataatgaagtggctcaagaatatgggcacgatattcataatatcgaccattgcttatcatatataacatctgtagatcatgactcaattaaagcgaaagagatttgccggaaatttctggggcattggtatgattcttatgtgaatgctacgactatttttgatgattcagaccaaacaagaggttatgatttcaataaagggcagtggcgtgactttgtattaaaaggacataaagatactaatcgccgtattgattacagttacgaaatcaatcccgtgggaacgccgcaggaatgtattgacataattcaaaaagacattgatgctacaggaatatcaaatatttgttgtggatttgaagctaatggaacagtagacgaaattattgcttccatgaagctcttccagtctgatgtcatgccatttcttaaagaaaaacaacgttcgctattatattagctaaggaggtaaagaaatgaaatttggattgttcttccttaacttcatcaattcaacaactgttcaagaacaaagtatagttcgcatgcaggaaataacggagtatgttgataagttgaattttgaacagattttagtgtatgaaaatcatttttcagataatggtgttgtcggcgctcctctgactgtttctggttttctgctcggtttaacagagaaaattaaaattggttcattaaatcacatcattacaactcatcatcctgtcgccatagcggaggaagcttgcttattggatcagttaagtgaagggagatttattttagggtttagtgattgcgaaaaaaaagatgaaatgcatttttttaatcgcccggttgaatatcaacagcaactatttgaagagtgttatgaaatcattaacgatgctttaacaacaggctattgtaatccagataacgatttttatagcttccctaaaatatctgtaaatccccatgcttatacgccaggcggacctcggaaatatgtaacagcaaccagtcatcatattgttgagtgggcggccaaaaaaggtattcctctcatctttaagtgggatgattctaatgatgttagatatgaatatgctgaaagatataaagccgttgcggataaatatgacgttgacctatcagagatagaccatcagttaatgatattagttaactataacgaagatagtaataaagctaaacaagagacgcgtgcatttattagtgattatgttcttgaaatgcaccctaatgaaaatttcgaaaataaacttgaagaaataattgcagaaaacgctgtcggaaattata…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K777133_sequence/1
type: Sequence
persistent identity: BBa K777133 sequence
display id: BBa_K777133_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: actagtgaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaagcggccgctgcagtccggcaaaaaagggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccacaggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagctcgaggcttggattctcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggagttctgaggtcattactggatctatcaac…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23018_sequence/1
type: Sequence
persistent identity: BBa J23018 sequence
display id: BBa_J23018_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagtagcggccgctgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtat…
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z