//plasmidbackbone/copynumber/high
type: Collection
persistent identity: plasmidbackbone copynumber high
display id: plasmidbackbone_copynumber_high
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: pSB1A3
member: BBa_K332033
member: pFun
member: BBa_K801003
member: BBa_K1680026
member: pSB1T3
member: Screening
member: pSB1AK3
member: pSB1C3
member: BBa_K332031
member: BBa_K801002
member: BBa_K598013
member: BBa_K598022
member: BBa_K2168001
member: BBa_K823055
member: pMam
member: pSB1AT3
member: BBa_K598000
member: BBa_K598019
member: pSB1K3
member: GluColi
member: BBa_K598014
member: BBa_K801001
member: pSB1A30
member: BBa_K2168000
member: BBa_K801000
member: BBa_K332032
member: BBa_K598002
member: pSB1A7
member: cry11Aa
member: BBa_K598020
member: pSB1AC3
member: BBa_K801004

BBa_K823055
type: ComponentDefinition
persistent identity: BBa K823055
display id: BBa_K823055
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB1C3F-Vector pSB1C3 with RFP-cassette in Freiburg Standard
creator: Jara Radeck
created: 2013-10-15T11:00:00Z
modified: 2015-05-08T01:13:30Z
discontinued: false
dominant: false
group u list: _1081_
m user id: 0
owner id: 6378
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To allow the possibility to save some nucleotides of primer-overhangs, you can use this vector. The two AgeI-restriction sites from the original RFP-cassette have been removed. The cassette is flanked by the modified Freiburg standard (http://2012.igem.org/wiki/images/b/b9/LMU-Munich_2012_Our_Freiburg_standard_FusionPrefix.pdf) that includes an optimal ribosome binding site between XbaI and NgoMIV restriction sites.
mutable notes: Two AgeI-restriction sites have been removed by two subsequent quikchange site-directed mutagnesis reactions. The RFP-cassette was PCR-amplified with the overhangs: prefix: GAATTCCGCGGCCGCTTCTAGATAAGGAGGAACTACTATGGCCGGC suffix: ACCGGTTAATACTAGTAGCGGCCGCTGCAGT
mutable provenance: pSB1C3; the RFP-part is originally from Discosoma sp.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: ori (pMB1)
sequence annotation: cat
sequence: https://synbiohub.org/public/igem/BBa_K823055_sequence/1

ori (pMB1)
type: SequenceAnnotation
persistent identity: annotation2371772
display id: annotation2371772
version: 1
top level: BBa_K823055
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823055/annotation2371772/range2371772/1

https://synbiohub.org/public/igem/BBa_K823055/annotation2371772/range2371772/1
type: Range
persistent identity: range2371772
display id: range2371772
version: 1
direction: direction/forward
top level: BBa_K823055
start: 260
end: 874
orientation: inline

cat
type: SequenceAnnotation
persistent identity: annotation2371771
display id: annotation2371771
version: 1
top level: BBa_K823055
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823055/annotation2371771/range2371771/1

https://synbiohub.org/public/igem/BBa_K823055/annotation2371771/range2371771/1
type: Range
persistent identity: range2371771
display id: range2371771
version: 1
direction: direction/reverse
top level: BBa_K823055
start: 1169
end: 1828
orientation: inline

BBa_K598020
type: ComponentDefinition
persistent identity: BBa K598020
display id: BBa_K598020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0015+pBAD+vioAB+B0015+pBAD+TPP Up-regulated Hammerhead Ribozyme 1.20+vioD+B0015+pBAD+vioE
creator: Zhenrun ZHANG
created: 2011-09-27T11:00:00Z
modified: 2015-05-08T01:12:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _770_
m user id: 0
owner id: 8606
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: B0015+pBAD+vioAB+B0015+pBAD+TPP ribozyme 1.20+vioD+B0015+pBAD+vioE
mutable notes: The Ribosomal Binding Site of vioD is contained in TPP ribozyme 1.20.
mutable provenance: The hammerhead ribozyme comes from E. coli, and the TPP aptamer comes from B. subtilis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: B0015
sequence annotation: vioE
sequence annotation: rbs
sequence annotation: pBAD
sequence annotation: vioD
sequence annotation: vioB
sequence annotation: vioA
sequence annotation: TPP ribozyme 1.20
sequence: https://synbiohub.org/public/igem/BBa_K598020_sequence/1

B0015
type: SequenceAnnotation
persistent identity: annotation2154583
display id: annotation2154583
version: 1
top level: BBa_K598020
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K598020/annotation2154583/range2154583/1

https://synbiohub.org/public/igem/BBa_K598020/annotation2154583/range2154583/1
type: Range
persistent identity: range2154583
display id: range2154583
version: 1
direction: direction/forward
top level: BBa_K598020
start: 6205
end: 6333
orientation: inline

vioE
type: SequenceAnnotation
persistent identity: annotation2154586
display id: annotation2154586
version: 1
top level: BBa_K598020
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K598020/annotation2154586/range2154586/1

https://synbiohub.org/public/igem/BBa_K598020/annotation2154586/range2154586/1
type: Range
persistent identity: range2154586
display id: range2154586
version: 1
direction: direction/forward
top level: BBa_K598020
start: 6489
end: 7064
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2154516
display id: annotation2154516
version: 1
top level: BBa_K598020
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598020/annotation2154516/range2154516/1

https://synbiohub.org/public/igem/BBa_K598020/annotation2154516/range2154516/1
type: Range
persistent identity: range2154516
display id: range2154516
version: 1
direction: direction/forward
top level: BBa_K598020
start: 273
end: 279
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2154588
display id: annotation2154588
version: 1
top level: BBa_K598020
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598020/annotation2154588/range2154588/1

https://synbiohub.org/public/igem/BBa_K598020/annotation2154588/range2154588/1
type: Range
persistent identity: range2154588
display id: range2154588
version: 1
direction: direction/forward
top level: BBa_K598020
start: 6477
end: 6483
orientation: inline

pBAD
type: SequenceAnnotation
persistent identity: annotation2154548
display id: annotation2154548
version: 1
top level: BBa_K598020
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K598020/annotation2154548/range2154548/1

https://synbiohub.org/public/igem/BBa_K598020/annotation2154548/range2154548/1
type: Range
persistent identity: range2154548
display id: range2154548
version: 1
direction: direction/forward
top level: BBa_K598020
start: 4702
end: 4831
orientation: inline

pBAD
type: SequenceAnnotation
persistent identity: annotation2154515
display id: annotation2154515
version: 1
top level: BBa_K598020
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K598020/annotation2154515/range2154515/1

https://synbiohub.org/public/igem/BBa_K598020/annotation2154515/range2154515/1
type: Range
persistent identity: range2154515
display id: range2154515
version: 1
direction: direction/forward
top level: BBa_K598020
start: 138
end: 267
orientation: inline

vioD
type: SequenceAnnotation
persistent identity: annotation2154576
display id: annotation2154576
version: 1
top level: BBa_K598020
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K598020/annotation2154576/range2154576/1

https://synbiohub.org/public/igem/BBa_K598020/annotation2154576/range2154576/1
type: Range
persistent identity: range2154576
display id: range2154576
version: 1
direction: direction/forward
top level: BBa_K598020
start: 4988
end: 6112
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2154514
display id: annotation2154514
version: 1
top level: BBa_K598020
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K598020/annotation2154514/range2154514/1

https://synbiohub.org/public/igem/BBa_K598020/annotation2154514/range2154514/1
type: Range
persistent identity: range2154514
display id: range2154514
version: 1
direction: direction/forward
top level: BBa_K598020
start: 1
end: 129
orientation: inline

vioB
type: SequenceAnnotation
persistent identity: annotation2154542
display id: annotation2154542
version: 1
top level: BBa_K598020
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K598020/annotation2154542/range2154542/1

https://synbiohub.org/public/igem/BBa_K598020/annotation2154542/range2154542/1
type: Range
persistent identity: range2154542
display id: range2154542
version: 1
direction: direction/forward
top level: BBa_K598020
start: 1559
end: 4556
orientation: inline

pBAD
type: SequenceAnnotation
persistent identity: annotation2154577
display id: annotation2154577
version: 1
top level: BBa_K598020
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K598020/annotation2154577/range2154577/1

https://synbiohub.org/public/igem/BBa_K598020/annotation2154577/range2154577/1
type: Range
persistent identity: range2154577
display id: range2154577
version: 1
direction: direction/forward
top level: BBa_K598020
start: 6342
end: 6471
orientation: inline

vioA
type: SequenceAnnotation
persistent identity: annotation2154517
display id: annotation2154517
version: 1
top level: BBa_K598020
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K598020/annotation2154517/range2154517/1

https://synbiohub.org/public/igem/BBa_K598020/annotation2154517/range2154517/1
type: Range
persistent identity: range2154517
display id: range2154517
version: 1
direction: direction/forward
top level: BBa_K598020
start: 285
end: 1542
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2154575
display id: annotation2154575
version: 1
top level: BBa_K598020
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598020/annotation2154575/range2154575/1

https://synbiohub.org/public/igem/BBa_K598020/annotation2154575/range2154575/1
type: Range
persistent identity: range2154575
display id: range2154575
version: 1
direction: direction/forward
top level: BBa_K598020
start: 4976
end: 4982
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2154518
display id: annotation2154518
version: 1
top level: BBa_K598020
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598020/annotation2154518/range2154518/1

https://synbiohub.org/public/igem/BBa_K598020/annotation2154518/range2154518/1
type: Range
persistent identity: range2154518
display id: range2154518
version: 1
direction: direction/forward
top level: BBa_K598020
start: 1545
end: 1551
orientation: inline

TPP ribozyme 1.20
type: SequenceAnnotation
persistent identity: annotation2154556
display id: annotation2154556
version: 1
top level: BBa_K598020
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K598020/annotation2154556/range2154556/1

https://synbiohub.org/public/igem/BBa_K598020/annotation2154556/range2154556/1
type: Range
persistent identity: range2154556
display id: range2154556
version: 1
direction: direction/forward
top level: BBa_K598020
start: 4838
end: 4987
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2154545
display id: annotation2154545
version: 1
top level: BBa_K598020
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K598020/annotation2154545/range2154545/1

https://synbiohub.org/public/igem/BBa_K598020/annotation2154545/range2154545/1
type: Range
persistent identity: range2154545
display id: range2154545
version: 1
direction: direction/forward
top level: BBa_K598020
start: 4565
end: 4693
orientation: inline

pSB1AC3
type: ComponentDefinition
persistent identity: pSB1AC3
display id: pSB1AC3
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: High copy BioBrick assembly plasmid
creator: Tom Knight
created: 2005-06-06T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _42_
m user id: 0
owner id: 6
owning group id: 42
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: pSB1AC3 is a high copy number plasmid carrying ampicillin and chloramphenicol resistance. The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell). For the version of this plasmid with a CcdB insert, see BBa_P1010.
mutable provenance: pSB1A3 with added Cm resistance
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: APr (rev)
sequence annotation: end of terminator
sequence annotation: terminator stem loop
sequence annotation: Prefix
sequence annotation: deletion of two bases
sequence annotation: VR
sequence annotation: Suffix
sequence annotation: ORI
sequence annotation: VF2
sequence annotation: E. coli his operon terminator
sequence annotation: T0 terminator
sequence annotation: Silent g->a mutation found during sequencing
sequence annotation: Cm R (rev)
sequence annotation: rep (pMB1) (rev)
sequence: https://synbiohub.org/public/igem/pSB1AC3_sequence/1

APr (rev)
type: SequenceAnnotation
persistent identity: annotation1779661
display id: annotation1779661
version: 1
top level: pSB1AC3
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB1AC3/annotation1779661/range1779661/1

https://synbiohub.org/public/igem/pSB1AC3/annotation1779661/range1779661/1
type: Range
persistent identity: range1779661
display id: range1779661
version: 1
direction: direction/reverse
top level: pSB1AC3
start: 1933
end: 2793
orientation: inline

end of terminator
type: SequenceAnnotation
persistent identity: annotation1823949
display id: annotation1823949
version: 1
top level: pSB1AC3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1AC3/annotation1823949/range1823949/1

https://synbiohub.org/public/igem/pSB1AC3/annotation1823949/range1823949/1
type: Range
persistent identity: range1823949
display id: range1823949
version: 1
direction: direction/reverse
top level: pSB1AC3
start: 2992
end: 2995
orientation: inline

terminator stem loop
type: SequenceAnnotation
persistent identity: annotation1779720
display id: annotation1779720
version: 1
top level: pSB1AC3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1AC3/annotation1779720/range1779720/1

https://synbiohub.org/public/igem/pSB1AC3/annotation1779720/range1779720/1
type: Range
persistent identity: range1779720
display id: range1779720
version: 1
direction: direction/reverse
top level: pSB1AC3
start: 3005
end: 3024
orientation: inline

Prefix
type: SequenceAnnotation
persistent identity: annotation1779618
display id: annotation1779618
version: 1
top level: pSB1AC3
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB1AC3/annotation1779618/range1779618/1

https://synbiohub.org/public/igem/pSB1AC3/annotation1779618/range1779618/1
type: Range
persistent identity: range1779618
display id: range1779618
version: 1
direction: direction/forward
top level: pSB1AC3
start: 3034
end: 3055
orientation: inline

deletion of two bases
type: SequenceAnnotation
persistent identity: annotation1823815
display id: annotation1823815
version: 1
top level: pSB1AC3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1AC3/annotation1823815/range1823815/1

https://synbiohub.org/public/igem/pSB1AC3/annotation1823815/range1823815/1
type: Range
persistent identity: range1823815
display id: range1823815
version: 1
direction: direction/forward
top level: pSB1AC3
start: 34
end: 35
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation1779731
display id: annotation1779731
version: 1
top level: pSB1AC3
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB1AC3/annotation1779731/range1779731/1

https://synbiohub.org/public/igem/pSB1AC3/annotation1779731/range1779731/1
type: Range
persistent identity: range1779731
display id: range1779731
version: 1
direction: direction/reverse
top level: pSB1AC3
start: 157
end: 176
orientation: inline

Suffix
type: SequenceAnnotation
persistent identity: annotation1779617
display id: annotation1779617
version: 1
top level: pSB1AC3
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB1AC3/annotation1779617/range1779617/1

https://synbiohub.org/public/igem/pSB1AC3/annotation1779617/range1779617/1
type: Range
persistent identity: range1779617
display id: range1779617
version: 1
direction: direction/forward
top level: pSB1AC3
start: 1
end: 21
orientation: inline

terminator stem loop
type: SequenceAnnotation
persistent identity: annotation1779665
display id: annotation1779665
version: 1
top level: pSB1AC3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1AC3/annotation1779665/range1779665/1

https://synbiohub.org/public/igem/pSB1AC3/annotation1779665/range1779665/1
type: Range
persistent identity: range1779665
display id: range1779665
version: 1
direction: direction/forward
top level: pSB1AC3
start: 30
end: 64
orientation: inline

ORI
type: SequenceAnnotation
persistent identity: annotation1779653
display id: annotation1779653
version: 1
top level: pSB1AC3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1AC3/annotation1779653/range1779653/1

https://synbiohub.org/public/igem/pSB1AC3/annotation1779653/range1779653/1
type: Range
persistent identity: range1779653
display id: range1779653
version: 1
direction: direction/forward
top level: pSB1AC3
start: 276
end: 276
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation1779730
display id: annotation1779730
version: 1
top level: pSB1AC3
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB1AC3/annotation1779730/range1779730/1

https://synbiohub.org/public/igem/pSB1AC3/annotation1779730/range1779730/1
type: Range
persistent identity: range1779730
display id: range1779730
version: 1
direction: direction/forward
top level: pSB1AC3
start: 2916
end: 2935
orientation: inline

E. coli his operon terminator
type: SequenceAnnotation
persistent identity: annotation1823948
display id: annotation1823948
version: 1
top level: pSB1AC3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1AC3/annotation1823948/range1823948/1

https://synbiohub.org/public/igem/pSB1AC3/annotation1823948/range1823948/1
type: Range
persistent identity: range1823948
display id: range1823948
version: 1
direction: direction/forward
top level: pSB1AC3
start: 22
end: 93
orientation: inline

T0 terminator
type: SequenceAnnotation
persistent identity: annotation1782172
display id: annotation1782172
version: 1
top level: pSB1AC3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1AC3/annotation1782172/range1782172/1

https://synbiohub.org/public/igem/pSB1AC3/annotation1782172/range1782172/1
type: Range
persistent identity: range1782172
display id: range1782172
version: 1
direction: direction/forward
top level: pSB1AC3
start: 1042
end: 1147
orientation: inline

Silent g->a mutation found during sequencing
type: SequenceAnnotation
persistent identity: annotation1782173
display id: annotation1782173
version: 1
top level: pSB1AC3
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/pSB1AC3/annotation1782173/range1782173/1

https://synbiohub.org/public/igem/pSB1AC3/annotation1782173/range1782173/1
type: Range
persistent identity: range1782173
display id: range1782173
version: 1
direction: direction/forward
top level: pSB1AC3
start: 1237
end: 1237
orientation: inline

Cm R (rev)
type: SequenceAnnotation
persistent identity: annotation1779657
display id: annotation1779657
version: 1
top level: pSB1AC3
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB1AC3/annotation1779657/range1779657/1

https://synbiohub.org/public/igem/pSB1AC3/annotation1779657/range1779657/1
type: Range
persistent identity: range1779657
display id: range1779657
version: 1
direction: direction/reverse
top level: pSB1AC3
start: 1163
end: 1821
orientation: inline

rep (pMB1) (rev)
type: SequenceAnnotation
persistent identity: annotation1779628
display id: annotation1779628
version: 1
top level: pSB1AC3
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB1AC3/annotation1779628/range1779628/1

https://synbiohub.org/public/igem/pSB1AC3/annotation1779628/range1779628/1
type: Range
persistent identity: range1779628
display id: range1779628
version: 1
direction: direction/reverse
top level: pSB1AC3
start: 261
end: 875
orientation: inline

BBa_K598013
type: ComponentDefinition
persistent identity: BBa K598013
display id: BBa_K598013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBAD+theoHH+CdIntron+E0040+B0015
creator: Xiaowei YAN, Tong MU
created: 2011-09-26T11:00:00Z
modified: 2015-05-08T01:12:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _770_
m user id: 0
owner id: 8713
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This a GFP reporter regulated by an allosteric intron responsive to theophylline.
mutable notes: The ligand response is alter from c-di-GMP to theophylline by merging a theophylline hammerhead ribozyme to the intron.
mutable provenance: The intron derives from the genome of Cd
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: theo HH
sequence annotation: RBS
sequence annotation: BBa_B0015
sequence annotation: pBAD
sequence annotation: eGFP
sequence annotation: group I intron
sequence: https://synbiohub.org/public/igem/BBa_K598013_sequence/1

theo HH
type: SequenceAnnotation
persistent identity: annotation2153072
display id: annotation2153072
version: 1
top level: BBa_K598013
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K598013/annotation2153072/range2153072/1

https://synbiohub.org/public/igem/BBa_K598013/annotation2153072/range2153072/1
type: Range
persistent identity: range2153072
display id: range2153072
version: 1
direction: direction/forward
top level: BBa_K598013
start: 139
end: 210
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2153079
display id: annotation2153079
version: 1
top level: BBa_K598013
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598013/annotation2153079/range2153079/1

https://synbiohub.org/public/igem/BBa_K598013/annotation2153079/range2153079/1
type: Range
persistent identity: range2153079
display id: range2153079
version: 1
direction: direction/forward
top level: BBa_K598013
start: 780
end: 780
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2153078
display id: annotation2153078
version: 1
top level: BBa_K598013
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598013/annotation2153078/range2153078/1

https://synbiohub.org/public/igem/BBa_K598013/annotation2153078/range2153078/1
type: Range
persistent identity: range2153078
display id: range2153078
version: 1
direction: direction/forward
top level: BBa_K598013
start: 212
end: 216
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2153077
display id: annotation2153077
version: 1
top level: BBa_K598013
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K598013/annotation2153077/range2153077/1

https://synbiohub.org/public/igem/BBa_K598013/annotation2153077/range2153077/1
type: Range
persistent identity: range2153077
display id: range2153077
version: 1
direction: direction/forward
top level: BBa_K598013
start: 1518
end: 1646
orientation: inline

pBAD
type: SequenceAnnotation
persistent identity: annotation2153071
display id: annotation2153071
version: 1
top level: BBa_K598013
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K598013/annotation2153071/range2153071/1

https://synbiohub.org/public/igem/BBa_K598013/annotation2153071/range2153071/1
type: Range
persistent identity: range2153071
display id: range2153071
version: 1
direction: direction/forward
top level: BBa_K598013
start: 1
end: 130
orientation: inline

eGFP
type: SequenceAnnotation
persistent identity: annotation2153074
display id: annotation2153074
version: 1
top level: BBa_K598013
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K598013/annotation2153074/range2153074/1

https://synbiohub.org/public/igem/BBa_K598013/annotation2153074/range2153074/1
type: Range
persistent identity: range2153074
display id: range2153074
version: 1
direction: direction/forward
top level: BBa_K598013
start: 790
end: 1509
orientation: inline

group I intron
type: SequenceAnnotation
persistent identity: annotation2153073
display id: annotation2153073
version: 1
top level: BBa_K598013
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K598013/annotation2153073/range2153073/1

https://synbiohub.org/public/igem/BBa_K598013/annotation2153073/range2153073/1
type: Range
persistent identity: range2153073
display id: range2153073
version: 1
direction: direction/forward
top level: BBa_K598013
start: 211
end: 789
orientation: inline

pFun
type: ComponentDefinition
persistent identity: BBa K678046
display id: BBa_K678046
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plasmid-Fun for Plug
creator: DTU-Denmark-2
created: 2011-09-04T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Plasmid for Plug'n'Play. Contains an Ampicillin resistance marker.
mutable notes: Compatible with the Plug'n'Play assembly standard.
mutable provenance: p71
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: pFun
sequence annotation: Linker 6
sequence: https://synbiohub.org/public/igem/BBa_K678046_sequence/1

pFun
type: SequenceAnnotation
persistent identity: annotation2127424
display id: annotation2127424
version: 1
top level: pFun
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678046/annotation2127424/range2127424/1

https://synbiohub.org/public/igem/BBa_K678046/annotation2127424/range2127424/1
type: Range
persistent identity: range2127424
display id: range2127424
version: 1
direction: direction/forward
top level: pFun
start: 9
end: 2878
orientation: inline

Linker 6
type: SequenceAnnotation
persistent identity: annotation2127423
display id: annotation2127423
version: 1
top level: pFun
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678046/annotation2127423/range2127423/1

https://synbiohub.org/public/igem/BBa_K678046/annotation2127423/range2127423/1
type: Range
persistent identity: range2127423
display id: range2127423
version: 1
direction: direction/forward
top level: pFun
start: 1
end: 8
orientation: inline

pSB1C3
type: ComponentDefinition
persistent identity: pSB1C3
display id: pSB1C3
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: High copy BioBrick assembly plasmid
creator: Austin Che
created: 2008-09-07T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a high copy plasmid for use in assembly of BioBrick® standard biological parts.
mutable notes: Eliminated ampicillin resistance.
mutable provenance: This part was derived from pSB1AC3-P1010.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: rep (pMB1)
sequence annotation: Chloramphenicol resistance
sequence annotation: BioBrick prefix
sequence annotation: T0 terminator
sequence annotation: end of terminator
sequence annotation: Verification forward (VF2) primer binding site
sequence annotation: E. coli his operon terminator
sequence annotation: BioBrick suffix
sequence annotation: Stem loop
sequence annotation: Verification reverse (VR) primer binding site
sequence annotation: ORI
sequence: https://synbiohub.org/public/igem/pSB1C3_sequence/1

rep (pMB1)
type: SequenceAnnotation
persistent identity: annotation2119042
display id: annotation2119042
version: 1
top level: pSB1C3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1C3/annotation2119042/range2119042/1

https://synbiohub.org/public/igem/pSB1C3/annotation2119042/range2119042/1
type: Range
persistent identity: range2119042
display id: range2119042
version: 1
direction: direction/reverse
top level: pSB1C3
start: 261
end: 875
orientation: inline

Chloramphenicol resistance
type: SequenceAnnotation
persistent identity: annotation2119045
display id: annotation2119045
version: 1
top level: pSB1C3
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB1C3/annotation2119045/range2119045/1

https://synbiohub.org/public/igem/pSB1C3/annotation2119045/range2119045/1
type: Range
persistent identity: range2119045
display id: range2119045
version: 1
direction: direction/reverse
top level: pSB1C3
start: 1160
end: 1819
orientation: inline

BioBrick prefix
type: SequenceAnnotation
persistent identity: annotation2119049
display id: annotation2119049
version: 1
top level: pSB1C3
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/pSB1C3/annotation2119049/range2119049/1

https://synbiohub.org/public/igem/pSB1C3/annotation2119049/range2119049/1
type: Range
persistent identity: range2119049
display id: range2119049
version: 1
direction: direction/forward
top level: pSB1C3
start: 2051
end: 2072
orientation: inline

T0 terminator
type: SequenceAnnotation
persistent identity: annotation2119044
display id: annotation2119044
version: 1
top level: pSB1C3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1C3/annotation2119044/range2119044/1

https://synbiohub.org/public/igem/pSB1C3/annotation2119044/range2119044/1
type: Range
persistent identity: range2119044
display id: range2119044
version: 1
direction: direction/forward
top level: pSB1C3
start: 1042
end: 1147
orientation: inline

end of terminator
type: SequenceAnnotation
persistent identity: annotation2119047
display id: annotation2119047
version: 1
top level: pSB1C3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1C3/annotation2119047/range2119047/1

https://synbiohub.org/public/igem/pSB1C3/annotation2119047/range2119047/1
type: Range
persistent identity: range2119047
display id: range2119047
version: 1
direction: direction/reverse
top level: pSB1C3
start: 2009
end: 2012
orientation: inline

Verification forward (VF2) primer binding site
type: SequenceAnnotation
persistent identity: annotation2119046
display id: annotation2119046
version: 1
top level: pSB1C3
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB1C3/annotation2119046/range2119046/1

https://synbiohub.org/public/igem/pSB1C3/annotation2119046/range2119046/1
type: Range
persistent identity: range2119046
display id: range2119046
version: 1
direction: direction/forward
top level: pSB1C3
start: 1933
end: 1952
orientation: inline

E. coli his operon terminator
type: SequenceAnnotation
persistent identity: annotation2119039
display id: annotation2119039
version: 1
top level: pSB1C3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1C3/annotation2119039/range2119039/1

https://synbiohub.org/public/igem/pSB1C3/annotation2119039/range2119039/1
type: Range
persistent identity: range2119039
display id: range2119039
version: 1
direction: direction/forward
top level: pSB1C3
start: 22
end: 93
orientation: inline

BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation2119038
display id: annotation2119038
version: 1
top level: pSB1C3
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/pSB1C3/annotation2119038/range2119038/1

https://synbiohub.org/public/igem/pSB1C3/annotation2119038/range2119038/1
type: Range
persistent identity: range2119038
display id: range2119038
version: 1
direction: direction/forward
top level: pSB1C3
start: 1
end: 21
orientation: inline

Stem loop
type: SequenceAnnotation
persistent identity: annotation2119040
display id: annotation2119040
version: 1
top level: pSB1C3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1C3/annotation2119040/range2119040/1

https://synbiohub.org/public/igem/pSB1C3/annotation2119040/range2119040/1
type: Range
persistent identity: range2119040
display id: range2119040
version: 1
direction: direction/forward
top level: pSB1C3
start: 30
end: 64
orientation: inline

Stem loop
type: SequenceAnnotation
persistent identity: annotation2119048
display id: annotation2119048
version: 1
top level: pSB1C3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1C3/annotation2119048/range2119048/1

https://synbiohub.org/public/igem/pSB1C3/annotation2119048/range2119048/1
type: Range
persistent identity: range2119048
display id: range2119048
version: 1
direction: direction/reverse
top level: pSB1C3
start: 2022
end: 2041
orientation: inline

Verification reverse (VR) primer binding site
type: SequenceAnnotation
persistent identity: annotation2119041
display id: annotation2119041
version: 1
top level: pSB1C3
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB1C3/annotation2119041/range2119041/1

https://synbiohub.org/public/igem/pSB1C3/annotation2119041/range2119041/1
type: Range
persistent identity: range2119041
display id: range2119041
version: 1
direction: direction/reverse
top level: pSB1C3
start: 157
end: 176
orientation: inline

ORI
type: SequenceAnnotation
persistent identity: annotation2119043
display id: annotation2119043
version: 1
top level: pSB1C3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1C3/annotation2119043/range2119043/1

https://synbiohub.org/public/igem/pSB1C3/annotation2119043/range2119043/1
type: Range
persistent identity: range2119043
display id: range2119043
version: 1
direction: direction/forward
top level: pSB1C3
start: 276
end: 276
orientation: inline

BBa_K598022
type: ComponentDefinition
persistent identity: BBa K598022
display id: BBa_K598022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Bistable Switch Mutant 42
creator: Yan GONG, Shuobin CHEN, Yunpeng LIU
created: 2011-09-26T11:00:00Z
modified: 2015-05-08T01:12:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _770_
m user id: 0
owner id: 8817
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: The RBS of CI434 is CGAGACC in this bistble switch, which makes the RFP expresses three times as the GFP does in this bistable switch.
mutable notes: The library is constructed by mutagenesis.
mutable provenance: Engineered
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: PRM (CI434 regulated promoter)
sequence annotation: lamda CI repressor
sequence annotation: RBS
sequence annotation: PR (CI regulated promoter)
sequence annotation: eGFP
sequence annotation: mRFP
sequence annotation: CI434 repressor
sequence: https://synbiohub.org/public/igem/BBa_K598022_sequence/1

PRM (CI434 regulated promoter)
type: SequenceAnnotation
persistent identity: annotation2150617
display id: annotation2150617
version: 1
top level: BBa_K598022
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K598022/annotation2150617/range2150617/1

https://synbiohub.org/public/igem/BBa_K598022/annotation2150617/range2150617/1
type: Range
persistent identity: range2150617
display id: range2150617
version: 1
direction: direction/reverse
top level: BBa_K598022
start: 1440
end: 1491
orientation: inline

lamda CI repressor
type: SequenceAnnotation
persistent identity: annotation2150615
display id: annotation2150615
version: 1
top level: BBa_K598022
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K598022/annotation2150615/range2150615/1

https://synbiohub.org/public/igem/BBa_K598022/annotation2150615/range2150615/1
type: Range
persistent identity: range2150615
display id: range2150615
version: 1
direction: direction/reverse
top level: BBa_K598022
start: 708
end: 1421
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2150616
display id: annotation2150616
version: 1
top level: BBa_K598022
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598022/annotation2150616/range2150616/1

https://synbiohub.org/public/igem/BBa_K598022/annotation2150616/range2150616/1
type: Range
persistent identity: range2150616
display id: range2150616
version: 1
direction: direction/reverse
top level: BBa_K598022
start: 1422
end: 1433
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2150614
display id: annotation2150614
version: 1
top level: BBa_K598022
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598022/annotation2150614/range2150614/1

https://synbiohub.org/public/igem/BBa_K598022/annotation2150614/range2150614/1
type: Range
persistent identity: range2150614
display id: range2150614
version: 1
direction: direction/reverse
top level: BBa_K598022
start: 682
end: 693
orientation: inline

PR (CI regulated promoter)
type: SequenceAnnotation
persistent identity: annotation2150618
display id: annotation2150618
version: 1
top level: BBa_K598022
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K598022/annotation2150618/range2150618/1

https://synbiohub.org/public/igem/BBa_K598022/annotation2150618/range2150618/1
type: Range
persistent identity: range2150618
display id: range2150618
version: 1
direction: direction/forward
top level: BBa_K598022
start: 1492
end: 1540
orientation: inline

eGFP
type: SequenceAnnotation
persistent identity: annotation2150622
display id: annotation2150622
version: 1
top level: BBa_K598022
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K598022/annotation2150622/range2150622/1

https://synbiohub.org/public/igem/BBa_K598022/annotation2150622/range2150622/1
type: Range
persistent identity: range2150622
display id: range2150622
version: 1
direction: direction/forward
top level: BBa_K598022
start: 2226
end: 2945
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2150619
display id: annotation2150619
version: 1
top level: BBa_K598022
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598022/annotation2150619/range2150619/1

https://synbiohub.org/public/igem/BBa_K598022/annotation2150619/range2150619/1
type: Range
persistent identity: range2150619
display id: range2150619
version: 1
direction: direction/forward
top level: BBa_K598022
start: 1556
end: 1567
orientation: inline

mRFP
type: SequenceAnnotation
persistent identity: annotation2150613
display id: annotation2150613
version: 1
top level: BBa_K598022
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K598022/annotation2150613/range2150613/1

https://synbiohub.org/public/igem/BBa_K598022/annotation2150613/range2150613/1
type: Range
persistent identity: range2150613
display id: range2150613
version: 1
direction: direction/reverse
top level: BBa_K598022
start: 1
end: 681
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2150621
display id: annotation2150621
version: 1
top level: BBa_K598022
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598022/annotation2150621/range2150621/1

https://synbiohub.org/public/igem/BBa_K598022/annotation2150621/range2150621/1
type: Range
persistent identity: range2150621
display id: range2150621
version: 1
direction: direction/forward
top level: BBa_K598022
start: 2214
end: 2225
orientation: inline

CI434 repressor
type: SequenceAnnotation
persistent identity: annotation2150620
display id: annotation2150620
version: 1
top level: BBa_K598022
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K598022/annotation2150620/range2150620/1

https://synbiohub.org/public/igem/BBa_K598022/annotation2150620/range2150620/1
type: Range
persistent identity: range2150620
display id: range2150620
version: 1
direction: direction/forward
top level: BBa_K598022
start: 1568
end: 2203
orientation: inline

pSB1A30
type: ComponentDefinition
persistent identity: BBa K1362091
display id: BBa_K1362091
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB1A30: High copy BioBrick cloning/expression backbone carrying Amp resitance
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1738_
m user id: 0
owner id: 22830
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: end of terminator
sequence annotation: VR
sequence annotation: terminator stem loop
sequence annotation: ORI
sequence annotation: T7 promotor
sequence annotation: E. coli his operon terminator
sequence annotation: prefix
sequence annotation: ampR (rev)
sequence annotation: rep(pMB1) (rev)
sequence annotation: lacO reg
sequence annotation: VF2
sequence annotation: suffix
sequence: https://synbiohub.org/public/igem/BBa_K1362091_sequence/1

end of terminator
type: SequenceAnnotation
persistent identity: annotation2404609
display id: annotation2404609
version: 1
top level: pSB1A30
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1362091/annotation2404609/range2404609/1

https://synbiohub.org/public/igem/BBa_K1362091/annotation2404609/range2404609/1
type: Range
persistent identity: range2404609
display id: range2404609
version: 1
direction: direction/reverse
top level: pSB1A30
start: 2094
end: 2097
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation2404602
display id: annotation2404602
version: 1
top level: pSB1A30
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K1362091/annotation2404602/range2404602/1

https://synbiohub.org/public/igem/BBa_K1362091/annotation2404602/range2404602/1
type: Range
persistent identity: range2404602
display id: range2404602
version: 1
direction: direction/reverse
top level: pSB1A30
start: 157
end: 176
orientation: inline

terminator stem loop
type: SequenceAnnotation
persistent identity: annotation2404610
display id: annotation2404610
version: 1
top level: pSB1A30
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1362091/annotation2404610/range2404610/1

https://synbiohub.org/public/igem/BBa_K1362091/annotation2404610/range2404610/1
type: Range
persistent identity: range2404610
display id: range2404610
version: 1
direction: direction/forward
top level: pSB1A30
start: 2107
end: 2126
orientation: inline

ORI
type: SequenceAnnotation
persistent identity: annotation2404606
display id: annotation2404606
version: 1
top level: pSB1A30
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1362091/annotation2404606/range2404606/1

https://synbiohub.org/public/igem/BBa_K1362091/annotation2404606/range2404606/1
type: Range
persistent identity: range2404606
display id: range2404606
version: 1
direction: direction/forward
top level: pSB1A30
start: 276
end: 276
orientation: inline

T7 promotor
type: SequenceAnnotation
persistent identity: annotation2404611
display id: annotation2404611
version: 1
top level: pSB1A30
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1362091/annotation2404611/range2404611/1

https://synbiohub.org/public/igem/BBa_K1362091/annotation2404611/range2404611/1
type: Range
persistent identity: range2404611
display id: range2404611
version: 1
direction: direction/forward
top level: pSB1A30
start: 2139
end: 2157
orientation: inline

E. coli his operon terminator
type: SequenceAnnotation
persistent identity: annotation2404601
display id: annotation2404601
version: 1
top level: pSB1A30
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1362091/annotation2404601/range2404601/1

https://synbiohub.org/public/igem/BBa_K1362091/annotation2404601/range2404601/1
type: Range
persistent identity: range2404601
display id: range2404601
version: 1
direction: direction/forward
top level: pSB1A30
start: 22
end: 93
orientation: inline

terminator stem loop
type: SequenceAnnotation
persistent identity: annotation2404603
display id: annotation2404603
version: 1
top level: pSB1A30
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1362091/annotation2404603/range2404603/1

https://synbiohub.org/public/igem/BBa_K1362091/annotation2404603/range2404603/1
type: Range
persistent identity: range2404603
display id: range2404603
version: 1
direction: direction/forward
top level: pSB1A30
start: 30
end: 64
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation2404632
display id: annotation2404632
version: 1
top level: pSB1A30
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1362091/annotation2404632/range2404632/1

https://synbiohub.org/public/igem/BBa_K1362091/annotation2404632/range2404632/1
type: Range
persistent identity: range2404632
display id: range2404632
version: 1
direction: direction/forward
top level: pSB1A30
start: 2185
end: 2206
orientation: inline

ampR (rev)
type: SequenceAnnotation
persistent identity: annotation2404607
display id: annotation2404607
version: 1
top level: pSB1A30
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1362091/annotation2404607/range2404607/1

https://synbiohub.org/public/igem/BBa_K1362091/annotation2404607/range2404607/1
type: Range
persistent identity: range2404607
display id: range2404607
version: 1
direction: direction/reverse
top level: pSB1A30
start: 1035
end: 1895
orientation: inline

rep(pMB1) (rev)
type: SequenceAnnotation
persistent identity: annotation2404605
display id: annotation2404605
version: 1
top level: pSB1A30
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1362091/annotation2404605/range2404605/1

https://synbiohub.org/public/igem/BBa_K1362091/annotation2404605/range2404605/1
type: Range
persistent identity: range2404605
display id: range2404605
version: 1
direction: direction/reverse
top level: pSB1A30
start: 261
end: 875
orientation: inline

lacO reg
type: SequenceAnnotation
persistent identity: annotation2404631
display id: annotation2404631
version: 1
top level: pSB1A30
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1362091/annotation2404631/range2404631/1

https://synbiohub.org/public/igem/BBa_K1362091/annotation2404631/range2404631/1
type: Range
persistent identity: range2404631
display id: range2404631
version: 1
direction: direction/forward
top level: pSB1A30
start: 2157
end: 2184
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation2404608
display id: annotation2404608
version: 1
top level: pSB1A30
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K1362091/annotation2404608/range2404608/1

https://synbiohub.org/public/igem/BBa_K1362091/annotation2404608/range2404608/1
type: Range
persistent identity: range2404608
display id: range2404608
version: 1
direction: direction/forward
top level: pSB1A30
start: 2018
end: 2037
orientation: inline

suffix
type: SequenceAnnotation
persistent identity: annotation2404600
display id: annotation2404600
version: 1
top level: pSB1A30
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1362091/annotation2404600/range2404600/1

https://synbiohub.org/public/igem/BBa_K1362091/annotation2404600/range2404600/1
type: Range
persistent identity: range2404600
display id: range2404600
version: 1
direction: direction/forward
top level: pSB1A30
start: 1
end: 21
orientation: inline

BBa_K801004
type: ComponentDefinition
persistent identity: BBa K801004
display id: BBa_K801004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pTUM104 yeast shuttle vector with GAL1 promoter
creator: Ingmar Polte
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1057_
m user id: 0
owner id: 13150
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is based on the plasmid pTUM100 (BBa_K801000). The galactose-inducible GAL1 yeast promoter has been cloned in front of the MCS (RFC25 prefix and suffix).
mutable notes: All forbidden restriction sites have been removed, making this plasmid RFC25-compatible.
mutable provenance: Based on invitrogen's pYES2 plasmid. For more information see the part information of pTUM100 (BBa_K801000).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: 2 ?? origin
sequence annotation: pUC1 origin
sequence annotation: URA3
sequence annotation: f1 origin
sequence annotation: CYC1 transcription terminator
sequence annotation: Ampicillin
sequence annotation: T7 promoter/priming site
sequence annotation: PGAL1
sequence: https://synbiohub.org/public/igem/BBa_K801004_sequence/1

2 ?? origin
type: SequenceAnnotation
persistent identity: annotation2213074
display id: annotation2213074
version: 1
top level: BBa_K801004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801004/annotation2213074/range2213074/1

https://synbiohub.org/public/igem/BBa_K801004/annotation2213074/range2213074/1
type: Range
persistent identity: range2213074
display id: range2213074
version: 1
direction: direction/forward
top level: BBa_K801004
start: 3281
end: 4752
orientation: inline

pUC1 origin
type: SequenceAnnotation
persistent identity: annotation2213071
display id: annotation2213071
version: 1
top level: BBa_K801004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801004/annotation2213071/range2213071/1

https://synbiohub.org/public/igem/BBa_K801004/annotation2213071/range2213071/1
type: Range
persistent identity: range2213071
display id: range2213071
version: 1
direction: direction/forward
top level: BBa_K801004
start: 474
end: 1147
orientation: inline

URA3
type: SequenceAnnotation
persistent identity: annotation2213073
display id: annotation2213073
version: 1
top level: BBa_K801004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801004/annotation2213073/range2213073/1

https://synbiohub.org/public/igem/BBa_K801004/annotation2213073/range2213073/1
type: Range
persistent identity: range2213073
display id: range2213073
version: 1
direction: direction/forward
top level: BBa_K801004
start: 2170
end: 3277
orientation: inline

f1 origin
type: SequenceAnnotation
persistent identity: annotation2213075
display id: annotation2213075
version: 1
top level: BBa_K801004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801004/annotation2213075/range2213075/1

https://synbiohub.org/public/igem/BBa_K801004/annotation2213075/range2213075/1
type: Range
persistent identity: range2213075
display id: range2213075
version: 1
direction: direction/forward
top level: BBa_K801004
start: 4820
end: 5275
orientation: inline

CYC1 transcription terminator
type: SequenceAnnotation
persistent identity: annotation2213070
display id: annotation2213070
version: 1
top level: BBa_K801004
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K801004/annotation2213070/range2213070/1

https://synbiohub.org/public/igem/BBa_K801004/annotation2213070/range2213070/1
type: Range
persistent identity: range2213070
display id: range2213070
version: 1
direction: direction/forward
top level: BBa_K801004
start: 44
end: 292
orientation: inline

Ampicillin
type: SequenceAnnotation
persistent identity: annotation2213072
display id: annotation2213072
version: 1
top level: BBa_K801004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801004/annotation2213072/range2213072/1

https://synbiohub.org/public/igem/BBa_K801004/annotation2213072/range2213072/1
type: Range
persistent identity: range2213072
display id: range2213072
version: 1
direction: direction/forward
top level: BBa_K801004
start: 1292
end: 2152
orientation: inline

T7 promoter/priming site
type: SequenceAnnotation
persistent identity: annotation2213077
display id: annotation2213077
version: 1
top level: BBa_K801004
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K801004/annotation2213077/range2213077/1

https://synbiohub.org/public/igem/BBa_K801004/annotation2213077/range2213077/1
type: Range
persistent identity: range2213077
display id: range2213077
version: 1
direction: direction/forward
top level: BBa_K801004
start: 5767
end: 5786
orientation: inline

PGAL1
type: SequenceAnnotation
persistent identity: annotation2213076
display id: annotation2213076
version: 1
top level: BBa_K801004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K801004/annotation2213076/range2213076/1

https://synbiohub.org/public/igem/BBa_K801004/annotation2213076/range2213076/1
type: Range
persistent identity: range2213076
display id: range2213076
version: 1
direction: direction/forward
top level: BBa_K801004
start: 5293
end: 5743
orientation: inline

BBa_K598019
type: ComponentDefinition
persistent identity: BBa K598019
display id: BBa_K598019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0015+pBAD+vioABD+B0015+pBAD+TPP Down-regulated Hammerhead Ribozyme 2.5+vioE
creator: Zhenrun ZHANG
created: 2011-09-26T11:00:00Z
modified: 2015-05-08T01:12:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _770_
m user id: 0
owner id: 8606
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: B0015+pBAD+vioABD+B0015+pBAD+TPP Down-regulated Hammerhead Ribozyme 2.5+vioE
mutable notes: the Ribosomal Binding Site of vioE is contained in TPP Ribozyme.
mutable provenance: Hammerhead Ribozyme backbone comes from E.coli, and TPP aptamer comes from B.subtilis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: TPP ribozyme 2.5
sequence annotation: vioB
sequence annotation: B0015
sequence annotation: vioA
sequence annotation: pBAD
sequence annotation: rbs
sequence annotation: vioD
sequence annotation: vioE
sequence: https://synbiohub.org/public/igem/BBa_K598019_sequence/1

TPP ribozyme 2.5
type: SequenceAnnotation
persistent identity: annotation2144624
display id: annotation2144624
version: 1
top level: BBa_K598019
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K598019/annotation2144624/range2144624/1

https://synbiohub.org/public/igem/BBa_K598019/annotation2144624/range2144624/1
type: Range
persistent identity: range2144624
display id: range2144624
version: 1
direction: direction/forward
top level: BBa_K598019
start: 5985
end: 6134
orientation: inline

vioB
type: SequenceAnnotation
persistent identity: annotation2144599
display id: annotation2144599
version: 1
top level: BBa_K598019
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K598019/annotation2144599/range2144599/1

https://synbiohub.org/public/igem/BBa_K598019/annotation2144599/range2144599/1
type: Range
persistent identity: range2144599
display id: range2144599
version: 1
direction: direction/forward
top level: BBa_K598019
start: 1559
end: 4556
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2144601
display id: annotation2144601
version: 1
top level: BBa_K598019
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K598019/annotation2144601/range2144601/1

https://synbiohub.org/public/igem/BBa_K598019/annotation2144601/range2144601/1
type: Range
persistent identity: range2144601
display id: range2144601
version: 1
direction: direction/forward
top level: BBa_K598019
start: 5712
end: 5841
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2144593
display id: annotation2144593
version: 1
top level: BBa_K598019
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K598019/annotation2144593/range2144593/1

https://synbiohub.org/public/igem/BBa_K598019/annotation2144593/range2144593/1
type: Range
persistent identity: range2144593
display id: range2144593
version: 1
direction: direction/forward
top level: BBa_K598019
start: 1
end: 129
orientation: inline

vioA
type: SequenceAnnotation
persistent identity: annotation2144597
display id: annotation2144597
version: 1
top level: BBa_K598019
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K598019/annotation2144597/range2144597/1

https://synbiohub.org/public/igem/BBa_K598019/annotation2144597/range2144597/1
type: Range
persistent identity: range2144597
display id: range2144597
version: 1
direction: direction/forward
top level: BBa_K598019
start: 285
end: 1542
orientation: inline

pBAD
type: SequenceAnnotation
persistent identity: annotation2144602
display id: annotation2144602
version: 1
top level: BBa_K598019
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K598019/annotation2144602/range2144602/1

https://synbiohub.org/public/igem/BBa_K598019/annotation2144602/range2144602/1
type: Range
persistent identity: range2144602
display id: range2144602
version: 1
direction: direction/forward
top level: BBa_K598019
start: 5849
end: 5978
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2144598
display id: annotation2144598
version: 1
top level: BBa_K598019
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598019/annotation2144598/range2144598/1

https://synbiohub.org/public/igem/BBa_K598019/annotation2144598/range2144598/1
type: Range
persistent identity: range2144598
display id: range2144598
version: 1
direction: direction/forward
top level: BBa_K598019
start: 1545
end: 1551
orientation: inline

vioD
type: SequenceAnnotation
persistent identity: annotation2144600
display id: annotation2144600
version: 1
top level: BBa_K598019
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K598019/annotation2144600/range2144600/1

https://synbiohub.org/public/igem/BBa_K598019/annotation2144600/range2144600/1
type: Range
persistent identity: range2144600
display id: range2144600
version: 1
direction: direction/forward
top level: BBa_K598019
start: 4579
end: 5704
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2144596
display id: annotation2144596
version: 1
top level: BBa_K598019
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598019/annotation2144596/range2144596/1

https://synbiohub.org/public/igem/BBa_K598019/annotation2144596/range2144596/1
type: Range
persistent identity: range2144596
display id: range2144596
version: 1
direction: direction/forward
top level: BBa_K598019
start: 273
end: 279
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2144630
display id: annotation2144630
version: 1
top level: BBa_K598019
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598019/annotation2144630/range2144630/1

https://synbiohub.org/public/igem/BBa_K598019/annotation2144630/range2144630/1
type: Range
persistent identity: range2144630
display id: range2144630
version: 1
direction: direction/forward
top level: BBa_K598019
start: 6122
end: 6129
orientation: inline

vioE
type: SequenceAnnotation
persistent identity: annotation2144631
display id: annotation2144631
version: 1
top level: BBa_K598019
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K598019/annotation2144631/range2144631/1

https://synbiohub.org/public/igem/BBa_K598019/annotation2144631/range2144631/1
type: Range
persistent identity: range2144631
display id: range2144631
version: 1
direction: direction/forward
top level: BBa_K598019
start: 6135
end: 6710
orientation: inline

pBAD
type: SequenceAnnotation
persistent identity: annotation2144595
display id: annotation2144595
version: 1
top level: BBa_K598019
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K598019/annotation2144595/range2144595/1

https://synbiohub.org/public/igem/BBa_K598019/annotation2144595/range2144595/1
type: Range
persistent identity: range2144595
display id: range2144595
version: 1
direction: direction/forward
top level: BBa_K598019
start: 138
end: 267
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2144594
display id: annotation2144594
version: 1
top level: BBa_K598019
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598019/annotation2144594/range2144594/1

https://synbiohub.org/public/igem/BBa_K598019/annotation2144594/range2144594/1
type: Range
persistent identity: range2144594
display id: range2144594
version: 1
direction: direction/forward
top level: BBa_K598019
start: 4565
end: 4571
orientation: inline

BBa_K801001
type: ComponentDefinition
persistent identity: BBa K801001
display id: BBa_K801001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pTUM101 yeast shuttle vector with pTEF1 promoter
creator: Georg Sch??tzinger
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1057_
m user id: 0
owner id: 13624
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part corresponds to pTUM100 (BBa_K801000). The constitutive pTEF1 promoter has been cloned in front of the MCS (RFC25 prefix and suffix).
mutable notes: All forbidden restriction sites have been removed from the plasmid backbone, making it RFC25 compatible
mutable provenance: Based on invitrogen's pYES2 plasmid. For mor information see the part information of pTUM100 (BBa_K801000).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: 2 micron (??) origin
sequence annotation: URA3
sequence annotation: CYC1-Terminator
sequence annotation: T7-Promoter
sequence annotation: pUC-origin
sequence annotation: TEF1-Promoter
sequence annotation: AmpR
sequence: https://synbiohub.org/public/igem/BBa_K801001_sequence/1

2 micron (??) origin
type: SequenceAnnotation
persistent identity: annotation2201773
display id: annotation2201773
version: 1
top level: BBa_K801001
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K801001/annotation2201773/range2201773/1

https://synbiohub.org/public/igem/BBa_K801001/annotation2201773/range2201773/1
type: Range
persistent identity: range2201773
display id: range2201773
version: 1
direction: direction/reverse
top level: BBa_K801001
start: 3777
end: 5248
orientation: inline

URA3
type: SequenceAnnotation
persistent identity: annotation2201772
display id: annotation2201772
version: 1
top level: BBa_K801001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801001/annotation2201772/range2201772/1

https://synbiohub.org/public/igem/BBa_K801001/annotation2201772/range2201772/1
type: Range
persistent identity: range2201772
display id: range2201772
version: 1
direction: direction/reverse
top level: BBa_K801001
start: 2666
end: 3773
orientation: inline

CYC1-Terminator
type: SequenceAnnotation
persistent identity: annotation2201769
display id: annotation2201769
version: 1
top level: BBa_K801001
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K801001/annotation2201769/range2201769/1

https://synbiohub.org/public/igem/BBa_K801001/annotation2201769/range2201769/1
type: Range
persistent identity: range2201769
display id: range2201769
version: 1
direction: direction/forward
top level: BBa_K801001
start: 540
end: 787
orientation: inline

T7-Promoter
type: SequenceAnnotation
persistent identity: annotation2201767
display id: annotation2201767
version: 1
top level: BBa_K801001
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K801001/annotation2201767/range2201767/1

https://synbiohub.org/public/igem/BBa_K801001/annotation2201767/range2201767/1
type: Range
persistent identity: range2201767
display id: range2201767
version: 1
direction: direction/forward
top level: BBa_K801001
start: 4
end: 23
orientation: inline

pUC-origin
type: SequenceAnnotation
persistent identity: annotation2201770
display id: annotation2201770
version: 1
top level: BBa_K801001
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K801001/annotation2201770/range2201770/1

https://synbiohub.org/public/igem/BBa_K801001/annotation2201770/range2201770/1
type: Range
persistent identity: range2201770
display id: range2201770
version: 1
direction: direction/reverse
top level: BBa_K801001
start: 970
end: 1643
orientation: inline

TEF1-Promoter
type: SequenceAnnotation
persistent identity: annotation2201768
display id: annotation2201768
version: 1
top level: BBa_K801001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K801001/annotation2201768/range2201768/1

https://synbiohub.org/public/igem/BBa_K801001/annotation2201768/range2201768/1
type: Range
persistent identity: range2201768
display id: range2201768
version: 1
direction: direction/forward
top level: BBa_K801001
start: 58
end: 505
orientation: inline

AmpR
type: SequenceAnnotation
persistent identity: annotation2201771
display id: annotation2201771
version: 1
top level: BBa_K801001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801001/annotation2201771/range2201771/1

https://synbiohub.org/public/igem/BBa_K801001/annotation2201771/range2201771/1
type: Range
persistent identity: range2201771
display id: range2201771
version: 1
direction: direction/reverse
top level: BBa_K801001
start: 1788
end: 2647
orientation: inline

pSB1T3
type: ComponentDefinition
persistent identity: pSB1T3
display id: pSB1T3
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: High copy BioBrick assembly plasmid
creator: Austin Che
created: 2008-09-07T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a high copy plasmid for use in assembly of BioBrick® standard biological parts.
mutable notes: The ampicillin resistance marker needed to be eliminated.
mutable provenance: Austin Che constructed this plamid from pSB1AT3-P1010.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: end of terminator
sequence annotation: Promoter P1
sequence annotation: ORI
sequence annotation: Tetracycline resistance
sequence annotation: Bind Site 1
sequence annotation: Stem loop
sequence annotation: C->T mutation
sequence annotation: Bind Site 5
sequence annotation: Verification reverse (VR) primer binding site
sequence annotation: Bind Site 2
sequence annotation: E. coli his operon terminator
sequence annotation: BioBrick prefix
sequence annotation: BioBrick suffix
sequence annotation: Bind Site 4
sequence annotation: Bind Site 3
sequence annotation: Verifiction forward (VF2) primer binding site
sequence annotation: rep (pMB1)
sequence annotation: Promoter P2
sequence annotation: two base pair AC deletion
sequence: https://synbiohub.org/public/igem/pSB1T3_sequence/1

end of terminator
type: SequenceAnnotation
persistent identity: annotation1975553
display id: annotation1975553
version: 1
top level: pSB1T3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1T3/annotation1975553/range1975553/1

https://synbiohub.org/public/igem/pSB1T3/annotation1975553/range1975553/1
type: Range
persistent identity: range1975553
display id: range1975553
version: 1
direction: direction/reverse
top level: pSB1T3
start: 2400
end: 2403
orientation: inline

Promoter P1
type: SequenceAnnotation
persistent identity: annotation1975543
display id: annotation1975543
version: 1
top level: pSB1T3
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB1T3/annotation1975543/range1975543/1

https://synbiohub.org/public/igem/pSB1T3/annotation1975543/range1975543/1
type: Range
persistent identity: range1975543
display id: range1975543
version: 1
direction: direction/reverse
top level: pSB1T3
start: 1068
end: 1074
orientation: inline

ORI
type: SequenceAnnotation
persistent identity: annotation1975541
display id: annotation1975541
version: 1
top level: pSB1T3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1T3/annotation1975541/range1975541/1

https://synbiohub.org/public/igem/pSB1T3/annotation1975541/range1975541/1
type: Range
persistent identity: range1975541
display id: range1975541
version: 1
direction: direction/forward
top level: pSB1T3
start: 276
end: 276
orientation: inline

Tetracycline resistance
type: SequenceAnnotation
persistent identity: annotation1975550
display id: annotation1975550
version: 1
top level: pSB1T3
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB1T3/annotation1975550/range1975550/1

https://synbiohub.org/public/igem/pSB1T3/annotation1975550/range1975550/1
type: Range
persistent identity: range1975550
display id: range1975550
version: 1
direction: direction/forward
top level: pSB1T3
start: 1127
end: 2317
orientation: inline

Bind Site 1
type: SequenceAnnotation
persistent identity: annotation1975544
display id: annotation1975544
version: 1
top level: pSB1T3
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/pSB1T3/annotation1975544/range1975544/1

https://synbiohub.org/public/igem/pSB1T3/annotation1975544/range1975544/1
type: Range
persistent identity: range1975544
display id: range1975544
version: 1
direction: direction/forward
top level: pSB1T3
start: 1065
end: 1068
orientation: inline

Stem loop
type: SequenceAnnotation
persistent identity: annotation1975538
display id: annotation1975538
version: 1
top level: pSB1T3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1T3/annotation1975538/range1975538/1

https://synbiohub.org/public/igem/pSB1T3/annotation1975538/range1975538/1
type: Range
persistent identity: range1975538
display id: range1975538
version: 1
direction: direction/forward
top level: pSB1T3
start: 30
end: 64
orientation: inline

C->T mutation
type: SequenceAnnotation
persistent identity: annotation1975551
display id: annotation1975551
version: 1
top level: pSB1T3
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB1T3/annotation1975551/range1975551/1

https://synbiohub.org/public/igem/pSB1T3/annotation1975551/range1975551/1
type: Range
persistent identity: range1975551
display id: range1975551
version: 1
direction: direction/forward
top level: pSB1T3
start: 2175
end: 2175
orientation: inline

Bind Site 5
type: SequenceAnnotation
persistent identity: annotation1975549
display id: annotation1975549
version: 1
top level: pSB1T3
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/pSB1T3/annotation1975549/range1975549/1

https://synbiohub.org/public/igem/pSB1T3/annotation1975549/range1975549/1
type: Range
persistent identity: range1975549
display id: range1975549
version: 1
direction: direction/forward
top level: pSB1T3
start: 1121
end: 1124
orientation: inline

Verification reverse (VR) primer binding site
type: SequenceAnnotation
persistent identity: annotation1975540
display id: annotation1975540
version: 1
top level: pSB1T3
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB1T3/annotation1975540/range1975540/1

https://synbiohub.org/public/igem/pSB1T3/annotation1975540/range1975540/1
type: Range
persistent identity: range1975540
display id: range1975540
version: 1
direction: direction/reverse
top level: pSB1T3
start: 157
end: 176
orientation: inline

Bind Site 2
type: SequenceAnnotation
persistent identity: annotation1975545
display id: annotation1975545
version: 1
top level: pSB1T3
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/pSB1T3/annotation1975545/range1975545/1

https://synbiohub.org/public/igem/pSB1T3/annotation1975545/range1975545/1
type: Range
persistent identity: range1975545
display id: range1975545
version: 1
direction: direction/forward
top level: pSB1T3
start: 1080
end: 1083
orientation: inline

E. coli his operon terminator
type: SequenceAnnotation
persistent identity: annotation1975539
display id: annotation1975539
version: 1
top level: pSB1T3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1T3/annotation1975539/range1975539/1

https://synbiohub.org/public/igem/pSB1T3/annotation1975539/range1975539/1
type: Range
persistent identity: range1975539
display id: range1975539
version: 1
direction: direction/forward
top level: pSB1T3
start: 22
end: 93
orientation: inline

BioBrick prefix
type: SequenceAnnotation
persistent identity: annotation1975555
display id: annotation1975555
version: 1
top level: pSB1T3
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/pSB1T3/annotation1975555/range1975555/1

https://synbiohub.org/public/igem/pSB1T3/annotation1975555/range1975555/1
type: Range
persistent identity: range1975555
display id: range1975555
version: 1
direction: direction/forward
top level: pSB1T3
start: 2442
end: 2463
orientation: inline

BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation1975537
display id: annotation1975537
version: 1
top level: pSB1T3
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/pSB1T3/annotation1975537/range1975537/1

https://synbiohub.org/public/igem/pSB1T3/annotation1975537/range1975537/1
type: Range
persistent identity: range1975537
display id: range1975537
version: 1
direction: direction/forward
top level: pSB1T3
start: 1
end: 21
orientation: inline

Bind Site 4
type: SequenceAnnotation
persistent identity: annotation1975548
display id: annotation1975548
version: 1
top level: pSB1T3
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/pSB1T3/annotation1975548/range1975548/1

https://synbiohub.org/public/igem/pSB1T3/annotation1975548/range1975548/1
type: Range
persistent identity: range1975548
display id: range1975548
version: 1
direction: direction/forward
top level: pSB1T3
start: 1108
end: 1111
orientation: inline

Bind Site 3
type: SequenceAnnotation
persistent identity: annotation1975546
display id: annotation1975546
version: 1
top level: pSB1T3
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/pSB1T3/annotation1975546/range1975546/1

https://synbiohub.org/public/igem/pSB1T3/annotation1975546/range1975546/1
type: Range
persistent identity: range1975546
display id: range1975546
version: 1
direction: direction/forward
top level: pSB1T3
start: 1094
end: 1097
orientation: inline

Stem loop
type: SequenceAnnotation
persistent identity: annotation1975554
display id: annotation1975554
version: 1
top level: pSB1T3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1T3/annotation1975554/range1975554/1

https://synbiohub.org/public/igem/pSB1T3/annotation1975554/range1975554/1
type: Range
persistent identity: range1975554
display id: range1975554
version: 1
direction: direction/reverse
top level: pSB1T3
start: 2413
end: 2432
orientation: inline

Verifiction forward (VF2) primer binding site
type: SequenceAnnotation
persistent identity: annotation1975552
display id: annotation1975552
version: 1
top level: pSB1T3
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB1T3/annotation1975552/range1975552/1

https://synbiohub.org/public/igem/pSB1T3/annotation1975552/range1975552/1
type: Range
persistent identity: range1975552
display id: range1975552
version: 1
direction: direction/forward
top level: pSB1T3
start: 2324
end: 2343
orientation: inline

rep (pMB1)
type: SequenceAnnotation
persistent identity: annotation1975542
display id: annotation1975542
version: 1
top level: pSB1T3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1T3/annotation1975542/range1975542/1

https://synbiohub.org/public/igem/pSB1T3/annotation1975542/range1975542/1
type: Range
persistent identity: range1975542
display id: range1975542
version: 1
direction: direction/reverse
top level: pSB1T3
start: 261
end: 875
orientation: inline

Promoter P2
type: SequenceAnnotation
persistent identity: annotation1975547
display id: annotation1975547
version: 1
top level: pSB1T3
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB1T3/annotation1975547/range1975547/1

https://synbiohub.org/public/igem/pSB1T3/annotation1975547/range1975547/1
type: Range
persistent identity: range1975547
display id: range1975547
version: 1
direction: direction/forward
top level: pSB1T3
start: 1084
end: 1090
orientation: inline

two base pair AC deletion
type: SequenceAnnotation
persistent identity: annotation1975536
display id: annotation1975536
version: 1
top level: pSB1T3
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB1T3/annotation1975536/range1975536/1

https://synbiohub.org/public/igem/pSB1T3/annotation1975536/range1975536/1
type: Range
persistent identity: range1975536
display id: range1975536
version: 1
direction: direction/forward
top level: pSB1T3
start: 34
end: 35
orientation: inline

BBa_K598002
type: ComponentDefinition
persistent identity: BBa K598002
display id: BBa_K598002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Bistable Switch Mutant 68
creator: Yan GONG, Shuobin CHEN, Yunpeng LIU, Xiaowei YAN
created: 2011-09-26T11:00:00Z
modified: 2015-05-08T01:12:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _770_
m user id: 0
owner id: 8683
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: RFP+RBS_SDA-C1+OR21 double directed promoter+SDcro CI434+eGFP
mutable notes: mutate the RBS seqence change the translation rate of CI434 to get the appropriate ratio of green and red.
mutable provenance: The part is made up of the standard parts from partsregistry, and some of the sub-parts are inverted by PCR.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: PRM (CI434 regulated promoter)
sequence annotation: PR (CI regulated promoter)
sequence annotation: eGFP
sequence annotation: RBS
sequence annotation: lamda CI repressor
sequence annotation: mRFP
sequence annotation: CI434 repressor
sequence: https://synbiohub.org/public/igem/BBa_K598002_sequence/1

PRM (CI434 regulated promoter)
type: SequenceAnnotation
persistent identity: annotation2150243
display id: annotation2150243
version: 1
top level: BBa_K598002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K598002/annotation2150243/range2150243/1

https://synbiohub.org/public/igem/BBa_K598002/annotation2150243/range2150243/1
type: Range
persistent identity: range2150243
display id: range2150243
version: 1
direction: direction/reverse
top level: BBa_K598002
start: 1440
end: 1491
orientation: inline

PR (CI regulated promoter)
type: SequenceAnnotation
persistent identity: annotation2150246
display id: annotation2150246
version: 1
top level: BBa_K598002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K598002/annotation2150246/range2150246/1

https://synbiohub.org/public/igem/BBa_K598002/annotation2150246/range2150246/1
type: Range
persistent identity: range2150246
display id: range2150246
version: 1
direction: direction/forward
top level: BBa_K598002
start: 1492
end: 1540
orientation: inline

eGFP
type: SequenceAnnotation
persistent identity: annotation2150250
display id: annotation2150250
version: 1
top level: BBa_K598002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K598002/annotation2150250/range2150250/1

https://synbiohub.org/public/igem/BBa_K598002/annotation2150250/range2150250/1
type: Range
persistent identity: range2150250
display id: range2150250
version: 1
direction: direction/forward
top level: BBa_K598002
start: 2226
end: 2945
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2150249
display id: annotation2150249
version: 1
top level: BBa_K598002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K598002/annotation2150249/range2150249/1

https://synbiohub.org/public/igem/BBa_K598002/annotation2150249/range2150249/1
type: Range
persistent identity: range2150249
display id: range2150249
version: 1
direction: direction/forward
top level: BBa_K598002
start: 2214
end: 2225
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2150247
display id: annotation2150247
version: 1
top level: BBa_K598002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598002/annotation2150247/range2150247/1

https://synbiohub.org/public/igem/BBa_K598002/annotation2150247/range2150247/1
type: Range
persistent identity: range2150247
display id: range2150247
version: 1
direction: direction/forward
top level: BBa_K598002
start: 1556
end: 1567
orientation: inline

lamda CI repressor
type: SequenceAnnotation
persistent identity: annotation2150238
display id: annotation2150238
version: 1
top level: BBa_K598002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K598002/annotation2150238/range2150238/1

https://synbiohub.org/public/igem/BBa_K598002/annotation2150238/range2150238/1
type: Range
persistent identity: range2150238
display id: range2150238
version: 1
direction: direction/reverse
top level: BBa_K598002
start: 708
end: 1421
orientation: inline

mRFP
type: SequenceAnnotation
persistent identity: annotation2150236
display id: annotation2150236
version: 1
top level: BBa_K598002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K598002/annotation2150236/range2150236/1

https://synbiohub.org/public/igem/BBa_K598002/annotation2150236/range2150236/1
type: Range
persistent identity: range2150236
display id: range2150236
version: 1
direction: direction/reverse
top level: BBa_K598002
start: 1
end: 681
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2150237
display id: annotation2150237
version: 1
top level: BBa_K598002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598002/annotation2150237/range2150237/1

https://synbiohub.org/public/igem/BBa_K598002/annotation2150237/range2150237/1
type: Range
persistent identity: range2150237
display id: range2150237
version: 1
direction: direction/reverse
top level: BBa_K598002
start: 682
end: 693
orientation: inline

CI434 repressor
type: SequenceAnnotation
persistent identity: annotation2150248
display id: annotation2150248
version: 1
top level: BBa_K598002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K598002/annotation2150248/range2150248/1

https://synbiohub.org/public/igem/BBa_K598002/annotation2150248/range2150248/1
type: Range
persistent identity: range2150248
display id: range2150248
version: 1
direction: direction/forward
top level: BBa_K598002
start: 1568
end: 2203
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2150239
display id: annotation2150239
version: 1
top level: BBa_K598002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598002/annotation2150239/range2150239/1

https://synbiohub.org/public/igem/BBa_K598002/annotation2150239/range2150239/1
type: Range
persistent identity: range2150239
display id: range2150239
version: 1
direction: direction/reverse
top level: BBa_K598002
start: 1422
end: 1433
orientation: inline

GluColi
type: ComponentDefinition
persistent identity: BBa K196014
display id: BBa_K196014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Final GluColi device.
creator: Laetitia Warny
created: 2009-10-17T11:00:00Z
modified: 2015-05-08T01:11:17Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _309_
m user id: 0
owner id: 4813
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is the part we actually created to test the glue production by E. coli. HfsG and HfsH come from Caulobacter crescentu. Caulobacter crescentus is an aquatic, Gram-negative bacterium that divides asymmetrically and is able to synthetize a strong glue. This glue is mainly made of a polysaccharide. There are different proteins needed to synthetize, export and attach it to the stalk of Caulobacter. To see the hole system, please see our wiki page [1]. In our project, we would like this glue to be produced by Escherichia coli. As E. coli does have homolog genes for many of these proteins, but not for HfsG and HfsH, we decided to create a plasmid including only the genes coding for these two proteins. HfsG is a glycosyltransferase and HfsH is a carbohydrate esterase. RFP has been added as a reporter.
mutable notes: As many mutations were needed to make the part compatible with the standard 10, we decided to make hfsG and hfsH synthetized by GeneArt [2]. We also optimized it for E. coli. We mean that the codons were changed to favour the most present in E. coli.
mutable provenance: HfsG and HfsH sequences come from Caulobacter crescentus.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K196007
component: BBa_K196005
sequence annotation: BBa_K196005
sequence annotation: BBa_K196007
sequence: https://synbiohub.org/public/igem/BBa_K196014_sequence/1

BBa_K196007
type: Component
persistent identity: component2267879
display id: component2267879
version: 1
top level: GluColi
access: public
definition: https://synbiohub.org/public/igem/BBa_K196007/1

BBa_K196005
type: Component
persistent identity: component2267888
display id: component2267888
version: 1
top level: GluColi
access: public
definition: https://synbiohub.org/public/igem/BBa_K196005/1

BBa_K196005
type: SequenceAnnotation
persistent identity: annotation2267888
display id: annotation2267888
version: 1
top level: GluColi
location: https://synbiohub.org/public/igem/BBa_K196014/annotation2267888/range2267888/1
component: BBa_K196005

https://synbiohub.org/public/igem/BBa_K196014/annotation2267888/range2267888/1
type: Range
persistent identity: range2267888
display id: range2267888
version: 1
direction: direction/reverse
top level: GluColi
start: 794
end: 2646
orientation: inline

BBa_K196007
type: SequenceAnnotation
persistent identity: annotation2267879
display id: annotation2267879
version: 1
top level: GluColi
location: https://synbiohub.org/public/igem/BBa_K196014/annotation2267879/range2267879/1
component: BBa_K196007

https://synbiohub.org/public/igem/BBa_K196014/annotation2267879/range2267879/1
type: Range
persistent identity: range2267879
display id: range2267879
version: 1
direction: direction/reverse
top level: GluColi
start: 1
end: 787
orientation: inline

pSB1K3
type: ComponentDefinition
persistent identity: pSB1K3
display id: pSB1K3
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: High copy BioBrick assembly plasmid
creator: Austin Che
created: 2008-09-07T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a high copy plasmid for use in assembly of BioBrick® standard biological parts.
mutable notes: Eliminated ampicillin resistance.
mutable provenance: This plasmid was derived from pSB1AK3-P1010.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Stem loop
sequence annotation: BioBrick prefix
sequence annotation: end of terminator
sequence annotation: Verification forward (VF2) primer binding site
sequence annotation: Verification reverse (VR) primer binding site
sequence annotation: Kanamycin resistance marker
sequence annotation: E. coli his operon terminator
sequence annotation: rep (pMB1)
sequence annotation: G->C mutation to remove XhoI site
sequence annotation: BioBrick suffix
sequence annotation: repeat region
sequence annotation: ORI
sequence: https://synbiohub.org/public/igem/pSB1K3_sequence/1

Stem loop
type: SequenceAnnotation
persistent identity: annotation2120011
display id: annotation2120011
version: 1
top level: pSB1K3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1K3/annotation2120011/range2120011/1

https://synbiohub.org/public/igem/pSB1K3/annotation2120011/range2120011/1
type: Range
persistent identity: range2120011
display id: range2120011
version: 1
direction: direction/forward
top level: pSB1K3
start: 30
end: 64
orientation: inline

BioBrick prefix
type: SequenceAnnotation
persistent identity: annotation2120021
display id: annotation2120021
version: 1
top level: pSB1K3
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/pSB1K3/annotation2120021/range2120021/1

https://synbiohub.org/public/igem/pSB1K3/annotation2120021/range2120021/1
type: Range
persistent identity: range2120021
display id: range2120021
version: 1
direction: direction/forward
top level: pSB1K3
start: 2185
end: 2206
orientation: inline

end of terminator
type: SequenceAnnotation
persistent identity: annotation2120019
display id: annotation2120019
version: 1
top level: pSB1K3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1K3/annotation2120019/range2120019/1

https://synbiohub.org/public/igem/pSB1K3/annotation2120019/range2120019/1
type: Range
persistent identity: range2120019
display id: range2120019
version: 1
direction: direction/reverse
top level: pSB1K3
start: 2143
end: 2146
orientation: inline

Verification forward (VF2) primer binding site
type: SequenceAnnotation
persistent identity: annotation2120018
display id: annotation2120018
version: 1
top level: pSB1K3
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB1K3/annotation2120018/range2120018/1

https://synbiohub.org/public/igem/pSB1K3/annotation2120018/range2120018/1
type: Range
persistent identity: range2120018
display id: range2120018
version: 1
direction: direction/forward
top level: pSB1K3
start: 2067
end: 2086
orientation: inline

Stem loop
type: SequenceAnnotation
persistent identity: annotation2120020
display id: annotation2120020
version: 1
top level: pSB1K3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1K3/annotation2120020/range2120020/1

https://synbiohub.org/public/igem/pSB1K3/annotation2120020/range2120020/1
type: Range
persistent identity: range2120020
display id: range2120020
version: 1
direction: direction/reverse
top level: pSB1K3
start: 2156
end: 2175
orientation: inline

Verification reverse (VR) primer binding site
type: SequenceAnnotation
persistent identity: annotation2120012
display id: annotation2120012
version: 1
top level: pSB1K3
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB1K3/annotation2120012/range2120012/1

https://synbiohub.org/public/igem/pSB1K3/annotation2120012/range2120012/1
type: Range
persistent identity: range2120012
display id: range2120012
version: 1
direction: direction/reverse
top level: pSB1K3
start: 157
end: 176
orientation: inline

Kanamycin resistance marker
type: SequenceAnnotation
persistent identity: annotation2120015
display id: annotation2120015
version: 1
top level: pSB1K3
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB1K3/annotation2120015/range2120015/1

https://synbiohub.org/public/igem/pSB1K3/annotation2120015/range2120015/1
type: Range
persistent identity: range2120015
display id: range2120015
version: 1
direction: direction/reverse
top level: pSB1K3
start: 1097
end: 1912
orientation: inline

E. coli his operon terminator
type: SequenceAnnotation
persistent identity: annotation2120010
display id: annotation2120010
version: 1
top level: pSB1K3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1K3/annotation2120010/range2120010/1

https://synbiohub.org/public/igem/pSB1K3/annotation2120010/range2120010/1
type: Range
persistent identity: range2120010
display id: range2120010
version: 1
direction: direction/forward
top level: pSB1K3
start: 22
end: 93
orientation: inline

rep (pMB1)
type: SequenceAnnotation
persistent identity: annotation2120013
display id: annotation2120013
version: 1
top level: pSB1K3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1K3/annotation2120013/range2120013/1

https://synbiohub.org/public/igem/pSB1K3/annotation2120013/range2120013/1
type: Range
persistent identity: range2120013
display id: range2120013
version: 1
direction: direction/reverse
top level: pSB1K3
start: 261
end: 875
orientation: inline

G->C mutation to remove XhoI site
type: SequenceAnnotation
persistent identity: annotation2120016
display id: annotation2120016
version: 1
top level: pSB1K3
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/pSB1K3/annotation2120016/range2120016/1

https://synbiohub.org/public/igem/pSB1K3/annotation2120016/range2120016/1
type: Range
persistent identity: range2120016
display id: range2120016
version: 1
direction: direction/reverse
top level: pSB1K3
start: 1880
end: 1880
orientation: inline

BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation2120009
display id: annotation2120009
version: 1
top level: pSB1K3
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/pSB1K3/annotation2120009/range2120009/1

https://synbiohub.org/public/igem/pSB1K3/annotation2120009/range2120009/1
type: Range
persistent identity: range2120009
display id: range2120009
version: 1
direction: direction/forward
top level: pSB1K3
start: 1
end: 21
orientation: inline

repeat region
type: SequenceAnnotation
persistent identity: annotation2120017
display id: annotation2120017
version: 1
top level: pSB1K3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1K3/annotation2120017/range2120017/1

https://synbiohub.org/public/igem/pSB1K3/annotation2120017/range2120017/1
type: Range
persistent identity: range2120017
display id: range2120017
version: 1
direction: direction/reverse
top level: pSB1K3
start: 2017
end: 2060
orientation: inline

ORI
type: SequenceAnnotation
persistent identity: annotation2120014
display id: annotation2120014
version: 1
top level: pSB1K3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1K3/annotation2120014/range2120014/1

https://synbiohub.org/public/igem/pSB1K3/annotation2120014/range2120014/1
type: Range
persistent identity: range2120014
display id: range2120014
version: 1
direction: direction/forward
top level: pSB1K3
start: 276
end: 276
orientation: inline

BBa_K1680026
type: ComponentDefinition
persistent identity: BBa K1680026
display id: BBa_K1680026
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pETUE
creator: Katharina Sporbeck
created: 2015-09-17T11:00:00Z
modified: 2015-10-28T03:58:14Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2098_
m user id: 15693
owner id: 26460
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Expression Plasmid coding for His-tag, also containing a thrombine cleavage site between coding region and Histag, so that His-tag can be cleaved off. Vecotr opening with PstI/XmaI, clone part into it with PstI/NgoMIV. Ampicillin resistance.
mutable notes: very strong RBS 6xHis-Tag Thromin-cleavage-site NgoMIV-compatible restriction site for in-frame insertion
mutable provenance: pET-Vector
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: T7 promoter
sequence annotation: thrombin cleavage site
sequence annotation: T7 terminator
sequence annotation: RBS.1 strong
sequence annotation: Lac operator
sequence annotation: 6x His-tag
sequence annotation: tet promoter
sequence annotation: f1 ori
sequence annotation: ori
sequence annotation: AmpR
sequence annotation: rrnB T1 terminator
sequence annotation: lac operator
sequence: https://synbiohub.org/public/igem/BBa_K1680026_sequence/1

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2472193
display id: annotation2472193
version: 1
top level: BBa_K1680026
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680026/annotation2472193/range2472193/1

https://synbiohub.org/public/igem/BBa_K1680026/annotation2472193/range2472193/1
type: Range
persistent identity: range2472193
display id: range2472193
version: 1
direction: direction/forward
top level: BBa_K1680026
start: 1
end: 19
orientation: inline

thrombin cleavage site
type: SequenceAnnotation
persistent identity: annotation2472237
display id: annotation2472237
version: 1
top level: BBa_K1680026
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1680026/annotation2472237/range2472237/1

https://synbiohub.org/public/igem/BBa_K1680026/annotation2472237/range2472237/1
type: Range
persistent identity: range2472237
display id: range2472237
version: 1
direction: direction/forward
top level: BBa_K1680026
start: 104
end: 121
orientation: inline

T7 terminator
type: SequenceAnnotation
persistent identity: annotation2472260
display id: annotation2472260
version: 1
top level: BBa_K1680026
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1680026/annotation2472260/range2472260/1

https://synbiohub.org/public/igem/BBa_K1680026/annotation2472260/range2472260/1
type: Range
persistent identity: range2472260
display id: range2472260
version: 1
direction: direction/forward
top level: BBa_K1680026
start: 683
end: 730
orientation: inline

RBS.1 strong
type: SequenceAnnotation
persistent identity: annotation2472195
display id: annotation2472195
version: 1
top level: BBa_K1680026
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1680026/annotation2472195/range2472195/1

https://synbiohub.org/public/igem/BBa_K1680026/annotation2472195/range2472195/1
type: Range
persistent identity: range2472195
display id: range2472195
version: 1
direction: direction/forward
top level: BBa_K1680026
start: 53
end: 67
orientation: inline

Lac operator
type: SequenceAnnotation
persistent identity: annotation2472194
display id: annotation2472194
version: 1
top level: BBa_K1680026
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1680026/annotation2472194/range2472194/1

https://synbiohub.org/public/igem/BBa_K1680026/annotation2472194/range2472194/1
type: Range
persistent identity: range2472194
display id: range2472194
version: 1
direction: direction/forward
top level: BBa_K1680026
start: 20
end: 44
orientation: inline

6x His-tag
type: SequenceAnnotation
persistent identity: annotation2472216
display id: annotation2472216
version: 1
top level: BBa_K1680026
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1680026/annotation2472216/range2472216/1

https://synbiohub.org/public/igem/BBa_K1680026/annotation2472216/range2472216/1
type: Range
persistent identity: range2472216
display id: range2472216
version: 1
direction: direction/forward
top level: BBa_K1680026
start: 77
end: 94
orientation: inline

tet promoter
type: SequenceAnnotation
persistent identity: annotation2472238
display id: annotation2472238
version: 1
top level: BBa_K1680026
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680026/annotation2472238/range2472238/1

https://synbiohub.org/public/igem/BBa_K1680026/annotation2472238/range2472238/1
type: Range
persistent identity: range2472238
display id: range2472238
version: 1
direction: direction/reverse
top level: BBa_K1680026
start: 212
end: 240
orientation: inline

f1 ori
type: SequenceAnnotation
persistent identity: annotation2472261
display id: annotation2472261
version: 1
top level: BBa_K1680026
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1680026/annotation2472261/range2472261/1

https://synbiohub.org/public/igem/BBa_K1680026/annotation2472261/range2472261/1
type: Range
persistent identity: range2472261
display id: range2472261
version: 1
direction: direction/forward
top level: BBa_K1680026
start: 767
end: 1222
orientation: inline

ori
type: SequenceAnnotation
persistent identity: annotation2472305
display id: annotation2472305
version: 1
top level: BBa_K1680026
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1680026/annotation2472305/range2472305/1

https://synbiohub.org/public/igem/BBa_K1680026/annotation2472305/range2472305/1
type: Range
persistent identity: range2472305
display id: range2472305
version: 1
direction: direction/forward
top level: BBa_K1680026
start: 2290
end: 2878
orientation: inline

AmpR
type: SequenceAnnotation
persistent identity: annotation2472289
display id: annotation2472289
version: 1
top level: BBa_K1680026
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1680026/annotation2472289/range2472289/1

https://synbiohub.org/public/igem/BBa_K1680026/annotation2472289/range2472289/1
type: Range
persistent identity: range2472289
display id: range2472289
version: 1
direction: direction/forward
top level: BBa_K1680026
start: 1337
end: 2197
orientation: inline

rrnB T1 terminator
type: SequenceAnnotation
persistent identity: annotation2472239
display id: annotation2472239
version: 1
top level: BBa_K1680026
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1680026/annotation2472239/range2472239/1

https://synbiohub.org/public/igem/BBa_K1680026/annotation2472239/range2472239/1
type: Range
persistent identity: range2472239
display id: range2472239
version: 1
direction: direction/forward
top level: BBa_K1680026
start: 410
end: 496
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation2472328
display id: annotation2472328
version: 1
top level: BBa_K1680026
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1680026/annotation2472328/range2472328/1

https://synbiohub.org/public/igem/BBa_K1680026/annotation2472328/range2472328/1
type: Range
persistent identity: range2472328
display id: range2472328
version: 1
direction: direction/forward
top level: BBa_K1680026
start: 3277
end: 3296
orientation: inline

BBa_K801000
type: ComponentDefinition
persistent identity: BBa K801000
display id: BBa_K801000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pTUM100 yeast shuttle vector based on pYES2
creator: Ingmar Polte
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1057_
m user id: 0
owner id: 13150
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: A yeast shuttle vector containing the following structural elements: * 2?? ori for replication in ''S. cerevisiae'' * URA3-gene for selection of transformed yeast via Uracil prototrophy * pUC ori for replication in ''E. coli'' * AmpR-gene for selection of transformed ''E. coli'' via Ampicillin resistance * f1 ori * MCS containing the RFC25 pre- and suffix for easy insertion of biobricks * following the MCS: CYC1 yeast transcriptional terminator Note: To use this backbone, a ''S. cerevisiae'' strain with Uracil-auxotrophy (such as strain INVSc1) is required for positive selection of transformants. Successfully transformed yeast cells will become prototrophic for uracil and can be selected on Agar-plates deficient of uracil.
mutable notes: -
mutable provenance: Based on invitrogen's pYES2-plasmid.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: URA3
sequence annotation: RFC25 MCS
sequence annotation: T7-Promoter
sequence annotation: 2 micron (??) origin
sequence annotation: Strep-tag II
sequence annotation: AmpR
sequence annotation: pUC-origin
sequence annotation: CYC1-Terminator
sequence: https://synbiohub.org/public/igem/BBa_K801000_sequence/1

URA3
type: SequenceAnnotation
persistent identity: annotation2202325
display id: annotation2202325
version: 1
top level: BBa_K801000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801000/annotation2202325/range2202325/1

https://synbiohub.org/public/igem/BBa_K801000/annotation2202325/range2202325/1
type: Range
persistent identity: range2202325
display id: range2202325
version: 1
direction: direction/reverse
top level: BBa_K801000
start: 2274
end: 3381
orientation: inline

RFC25 MCS
type: SequenceAnnotation
persistent identity: annotation2202314
display id: annotation2202314
version: 1
top level: BBa_K801000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801000/annotation2202314/range2202314/1

https://synbiohub.org/public/igem/BBa_K801000/annotation2202314/range2202314/1
type: Range
persistent identity: range2202314
display id: range2202314
version: 1
direction: direction/forward
top level: BBa_K801000
start: 36
end: 134
orientation: inline

T7-Promoter
type: SequenceAnnotation
persistent identity: annotation2202258
display id: annotation2202258
version: 1
top level: BBa_K801000
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K801000/annotation2202258/range2202258/1

https://synbiohub.org/public/igem/BBa_K801000/annotation2202258/range2202258/1
type: Range
persistent identity: range2202258
display id: range2202258
version: 1
direction: direction/forward
top level: BBa_K801000
start: 4
end: 23
orientation: inline

2 micron (??) origin
type: SequenceAnnotation
persistent identity: annotation2202326
display id: annotation2202326
version: 1
top level: BBa_K801000
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K801000/annotation2202326/range2202326/1

https://synbiohub.org/public/igem/BBa_K801000/annotation2202326/range2202326/1
type: Range
persistent identity: range2202326
display id: range2202326
version: 1
direction: direction/forward
top level: BBa_K801000
start: 3385
end: 4856
orientation: inline

Strep-tag II
type: SequenceAnnotation
persistent identity: annotation2202315
display id: annotation2202315
version: 1
top level: BBa_K801000
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K801000/annotation2202315/range2202315/1

https://synbiohub.org/public/igem/BBa_K801000/annotation2202315/range2202315/1
type: Range
persistent identity: range2202315
display id: range2202315
version: 1
direction: direction/forward
top level: BBa_K801000
start: 78
end: 101
orientation: inline

AmpR
type: SequenceAnnotation
persistent identity: annotation2202324
display id: annotation2202324
version: 1
top level: BBa_K801000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801000/annotation2202324/range2202324/1

https://synbiohub.org/public/igem/BBa_K801000/annotation2202324/range2202324/1
type: Range
persistent identity: range2202324
display id: range2202324
version: 1
direction: direction/reverse
top level: BBa_K801000
start: 1396
end: 2256
orientation: inline

pUC-origin
type: SequenceAnnotation
persistent identity: annotation2202317
display id: annotation2202317
version: 1
top level: BBa_K801000
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K801000/annotation2202317/range2202317/1

https://synbiohub.org/public/igem/BBa_K801000/annotation2202317/range2202317/1
type: Range
persistent identity: range2202317
display id: range2202317
version: 1
direction: direction/forward
top level: BBa_K801000
start: 578
end: 1251
orientation: inline

CYC1-Terminator
type: SequenceAnnotation
persistent identity: annotation2202316
display id: annotation2202316
version: 1
top level: BBa_K801000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K801000/annotation2202316/range2202316/1

https://synbiohub.org/public/igem/BBa_K801000/annotation2202316/range2202316/1
type: Range
persistent identity: range2202316
display id: range2202316
version: 1
direction: direction/forward
top level: BBa_K801000
start: 148
end: 396
orientation: inline

BBa_K2168001
type: ComponentDefinition
persistent identity: BBa K2168001
display id: BBa_K2168001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBAD/araC - (N-6xHis) - Ag43 - sfGFP
creator: Arunavo Chakraborty
created: 2016-10-12T11:00:00Z
modified: 2016-10-13T03:15:53Z
discontinued: false
dominant: false
group u list: _2639_
m user id: 30486
owner id: 30486
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This is a fusion protein with Ag43, a protein from E coli tagged with a 6xHis tag on the N-terminal and sfGFP tagged on C-terminal of the pro-protein. Ag43 causes cell-cell adhesion in E coli, and the 6xHis and sfGFP tags are present to allow convenient study dependence of Ag43 mediated aggregation on Ag43 expression levels, which can be determined from the sfGFP fluoresence and/or quantification of 6xHis tag. The 6xHis tag is included to allow protein concentration estimation in case the sfGFP fails to fold properly.
mutable notes: The location for the 6xHis tag is the same as that for BBa_K2168000(Ag43 tagged with 6xHis on N-terminal under pBAD/araC promoter) and uses the same design principles. For independent folding, a flexible GGGGSGGGGS linker was added between Ag43 and sfGFP, and the linker was choosen to be not very hydrophilic or hydrophobic, since Ag43 lies very close to lipid membranes.
mutable provenance: This part was made from Gibson assemblies from fragments of two previous parts - BBa_K1352000 and BBa_I746908, which code for Ag43 and sfGFP respectively.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: 6xHis
sequence annotation: B0034
sequence annotation: Double terminator (B0015)
sequence annotation: (GGGGS)2 linker
sequence annotation: sfGFP
sequence annotation: Ag43(PstI sites removed)
sequence annotation: pBAD
sequence annotation: araC
sequence: https://synbiohub.org/public/igem/BBa_K2168001_sequence/1

6xHis
type: SequenceAnnotation
persistent identity: annotation2499490
display id: annotation2499490
version: 1
top level: BBa_K2168001
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K2168001/annotation2499490/range2499490/1

https://synbiohub.org/public/igem/BBa_K2168001/annotation2499490/range2499490/1
type: Range
persistent identity: range2499490
display id: range2499490
version: 1
direction: direction/forward
top level: BBa_K2168001
start: 1396
end: 1413
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2499485
display id: annotation2499485
version: 1
top level: BBa_K2168001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K2168001/annotation2499485/range2499485/1

https://synbiohub.org/public/igem/BBa_K2168001/annotation2499485/range2499485/1
type: Range
persistent identity: range2499485
display id: range2499485
version: 1
direction: direction/forward
top level: BBa_K2168001
start: 1219
end: 1230
orientation: inline

Double terminator (B0015)
type: SequenceAnnotation
persistent identity: annotation2499489
display id: annotation2499489
version: 1
top level: BBa_K2168001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K2168001/annotation2499489/range2499489/1

https://synbiohub.org/public/igem/BBa_K2168001/annotation2499489/range2499489/1
type: Range
persistent identity: range2499489
display id: range2499489
version: 1
direction: direction/forward
top level: BBa_K2168001
start: 5130
end: 5258
orientation: inline

(GGGGS)2 linker
type: SequenceAnnotation
persistent identity: annotation2499487
display id: annotation2499487
version: 1
top level: BBa_K2168001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K2168001/annotation2499487/range2499487/1

https://synbiohub.org/public/igem/BBa_K2168001/annotation2499487/range2499487/1
type: Range
persistent identity: range2499487
display id: range2499487
version: 1
direction: direction/forward
top level: BBa_K2168001
start: 4372
end: 4401
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2499488
display id: annotation2499488
version: 1
top level: BBa_K2168001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K2168001/annotation2499488/range2499488/1

https://synbiohub.org/public/igem/BBa_K2168001/annotation2499488/range2499488/1
type: Range
persistent identity: range2499488
display id: range2499488
version: 1
direction: direction/forward
top level: BBa_K2168001
start: 4402
end: 5118
orientation: inline

Ag43(PstI sites removed)
type: SequenceAnnotation
persistent identity: annotation2499486
display id: annotation2499486
version: 1
top level: BBa_K2168001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K2168001/annotation2499486/range2499486/1

https://synbiohub.org/public/igem/BBa_K2168001/annotation2499486/range2499486/1
type: Range
persistent identity: range2499486
display id: range2499486
version: 1
direction: direction/forward
top level: BBa_K2168001
start: 1237
end: 4371
orientation: inline

pBAD
type: SequenceAnnotation
persistent identity: annotation2499484
display id: annotation2499484
version: 1
top level: BBa_K2168001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K2168001/annotation2499484/range2499484/1

https://synbiohub.org/public/igem/BBa_K2168001/annotation2499484/range2499484/1
type: Range
persistent identity: range2499484
display id: range2499484
version: 1
direction: direction/forward
top level: BBa_K2168001
start: 906
end: 1190
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation2499465
display id: annotation2499465
version: 1
top level: BBa_K2168001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K2168001/annotation2499465/range2499465/1

https://synbiohub.org/public/igem/BBa_K2168001/annotation2499465/range2499465/1
type: Range
persistent identity: range2499465
display id: range2499465
version: 1
direction: direction/reverse
top level: BBa_K2168001
start: 1
end: 879
orientation: inline

BBa_K332032
type: ComponentDefinition
persistent identity: BBa K332032
display id: BBa_K332032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutive promoter+37℃ induced RBS+tetR+double terminator+Ptet+RBS+GFP+double terminator
creator: KUO, CHIA-CHUN
created: 2010-10-21T11:00:00Z
modified: 2015-05-08T01:12:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _452_
m user id: 0
owner id: 7059
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: We want to utilize a temperature-regulated mechanism to control the expression period of a crystal protein. Therefore, we took advantage of the interaction between 37?? C induced RBS, tetR gene and pTet promoter to create the temperature regulating circuit. The following is a description of our prescribed strategy. First, we use the constitutive promoter, 37?? C induced RBS (K115002), the tetR gene and terminators. We will call this part the ???Regulator???.
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_R0040
component: BBa_K332031
component: BBa_B0034
component: BBa_E0040
component: BBa_J61048
sequence annotation: BBa_E0040
sequence annotation: BBa_K332031
sequence annotation: BBa_B0034
sequence annotation: BBa_R0040
sequence annotation: BBa_J61048
sequence: https://synbiohub.org/public/igem/BBa_K332032_sequence/1

BBa_R0040
type: Component
persistent identity: component2273247
display id: component2273247
version: 1
top level: BBa_K332032
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_K332031
type: Component
persistent identity: component2273246
display id: component2273246
version: 1
top level: BBa_K332032
access: public
definition: BBa_K332031

BBa_B0034
type: Component
persistent identity: component2273253
display id: component2273253
version: 1
top level: BBa_K332032
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0040
type: Component
persistent identity: component2273255
display id: component2273255
version: 1
top level: BBa_K332032
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_J61048
type: Component
persistent identity: component2273256
display id: component2273256
version: 1
top level: BBa_K332032
access: public
definition: https://synbiohub.org/public/igem/BBa_J61048/1

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2273255
display id: annotation2273255
version: 1
top level: BBa_K332032
location: https://synbiohub.org/public/igem/BBa_K332032/annotation2273255/range2273255/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K332032/annotation2273255/range2273255/1
type: Range
persistent identity: range2273255
display id: range2273255
version: 1
direction: direction/reverse
top level: BBa_K332032
start: 996
end: 1715
orientation: inline

BBa_K332031
type: SequenceAnnotation
persistent identity: annotation2273246
display id: annotation2273246
version: 1
top level: BBa_K332032
location: https://synbiohub.org/public/igem/BBa_K332032/annotation2273246/range2273246/1
component: BBa_K332031

https://synbiohub.org/public/igem/BBa_K332032/annotation2273246/range2273246/1
type: Range
persistent identity: range2273246
display id: range2273246
version: 1
direction: direction/reverse
top level: BBa_K332032
start: 1
end: 907
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2273253
display id: annotation2273253
version: 1
top level: BBa_K332032
location: https://synbiohub.org/public/igem/BBa_K332032/annotation2273253/range2273253/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K332032/annotation2273253/range2273253/1
type: Range
persistent identity: range2273253
display id: range2273253
version: 1
direction: direction/reverse
top level: BBa_K332032
start: 978
end: 989
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2273247
display id: annotation2273247
version: 1
top level: BBa_K332032
location: https://synbiohub.org/public/igem/BBa_K332032/annotation2273247/range2273247/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K332032/annotation2273247/range2273247/1
type: Range
persistent identity: range2273247
display id: range2273247
version: 1
direction: direction/forward
top level: BBa_K332032
start: 916
end: 969
orientation: inline

BBa_J61048
type: SequenceAnnotation
persistent identity: annotation2273256
display id: annotation2273256
version: 1
top level: BBa_K332032
location: https://synbiohub.org/public/igem/BBa_K332032/annotation2273256/range2273256/1
component: BBa_J61048

https://synbiohub.org/public/igem/BBa_K332032/annotation2273256/range2273256/1
type: Range
persistent identity: range2273256
display id: range2273256
version: 1
direction: direction/reverse
top level: BBa_K332032
start: 1724
end: 1836
orientation: inline

BBa_K801003
type: ComponentDefinition
persistent identity: BBa K801003
display id: BBa_K801003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pTUM103 yeast shuttle vector with pADH1 promoter
creator: Georg Sch??tzinger
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1057_
m user id: 0
owner id: 13624
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part corresponds to pTUM100 (BBa_K801000). The constitutive pADH1 promoter has been cloned in front of the MCS (RFC25 prefix and suffix).
mutable notes: All forbidden restriction sites have been removed from the plasmid backbone, making it RFC25 compatible .
mutable provenance: Based on invitrogen's pYES2 plasmid. For more information see the part information of pTUM100 (BBa_K801000).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: 2 micron (??) origin
sequence annotation: URA3
sequence annotation: pUC-origin
sequence annotation: AmpR
sequence annotation: T7-Promoter
sequence annotation: ADH1-Promoter
sequence annotation: CYC1-Terminator
sequence: https://synbiohub.org/public/igem/BBa_K801003_sequence/1

2 micron (??) origin
type: SequenceAnnotation
persistent identity: annotation2201910
display id: annotation2201910
version: 1
top level: BBa_K801003
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K801003/annotation2201910/range2201910/1

https://synbiohub.org/public/igem/BBa_K801003/annotation2201910/range2201910/1
type: Range
persistent identity: range2201910
display id: range2201910
version: 1
direction: direction/reverse
top level: BBa_K801003
start: 4047
end: 5518
orientation: inline

URA3
type: SequenceAnnotation
persistent identity: annotation2201841
display id: annotation2201841
version: 1
top level: BBa_K801003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801003/annotation2201841/range2201841/1

https://synbiohub.org/public/igem/BBa_K801003/annotation2201841/range2201841/1
type: Range
persistent identity: range2201841
display id: range2201841
version: 1
direction: direction/reverse
top level: BBa_K801003
start: 2936
end: 4043
orientation: inline

pUC-origin
type: SequenceAnnotation
persistent identity: annotation2201911
display id: annotation2201911
version: 1
top level: BBa_K801003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801003/annotation2201911/range2201911/1

https://synbiohub.org/public/igem/BBa_K801003/annotation2201911/range2201911/1
type: Range
persistent identity: range2201911
display id: range2201911
version: 1
direction: direction/reverse
top level: BBa_K801003
start: 1240
end: 1913
orientation: inline

AmpR
type: SequenceAnnotation
persistent identity: annotation2201842
display id: annotation2201842
version: 1
top level: BBa_K801003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801003/annotation2201842/range2201842/1

https://synbiohub.org/public/igem/BBa_K801003/annotation2201842/range2201842/1
type: Range
persistent identity: range2201842
display id: range2201842
version: 1
direction: direction/reverse
top level: BBa_K801003
start: 2058
end: 2917
orientation: inline

T7-Promoter
type: SequenceAnnotation
persistent identity: annotation2201845
display id: annotation2201845
version: 1
top level: BBa_K801003
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K801003/annotation2201845/range2201845/1

https://synbiohub.org/public/igem/BBa_K801003/annotation2201845/range2201845/1
type: Range
persistent identity: range2201845
display id: range2201845
version: 1
direction: direction/forward
top level: BBa_K801003
start: 4
end: 23
orientation: inline

ADH1-Promoter
type: SequenceAnnotation
persistent identity: annotation2201844
display id: annotation2201844
version: 1
top level: BBa_K801003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K801003/annotation2201844/range2201844/1

https://synbiohub.org/public/igem/BBa_K801003/annotation2201844/range2201844/1
type: Range
persistent identity: range2201844
display id: range2201844
version: 1
direction: direction/forward
top level: BBa_K801003
start: 58
end: 775
orientation: inline

CYC1-Terminator
type: SequenceAnnotation
persistent identity: annotation2201840
display id: annotation2201840
version: 1
top level: BBa_K801003
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K801003/annotation2201840/range2201840/1

https://synbiohub.org/public/igem/BBa_K801003/annotation2201840/range2201840/1
type: Range
persistent identity: range2201840
display id: range2201840
version: 1
direction: direction/forward
top level: BBa_K801003
start: 810
end: 1057
orientation: inline

pSB1AT3
type: ComponentDefinition
persistent identity: pSB1AT3
display id: pSB1AT3
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: High copy BioBrick assembly plasmid
creator: Tom Knight
created: 2005-06-06T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_48_1_
m user id: 0
owner id: 6
owning group id: 42
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: pSB1AT3 is a high copy number plasmid carrying ampicillin and tetracycline resistance. The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell).
mutable provenance: pSB1A3 with added Tet resistance
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: deletion of two bases
sequence annotation: ORI
sequence annotation: Prefix
sequence annotation: terminator stem loop
sequence annotation: end of terminator
sequence annotation: Tet-r
sequence annotation: Suffix
sequence annotation: E. coli his operon terminator
sequence annotation: rep (pMB1) (rev)
sequence annotation: VR
sequence annotation: APr (rev)
sequence annotation: VF2
sequence: https://synbiohub.org/public/igem/pSB1AT3_sequence/1

deletion of two bases
type: SequenceAnnotation
persistent identity: annotation1823801
display id: annotation1823801
version: 1
top level: pSB1AT3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1AT3/annotation1823801/range1823801/1

https://synbiohub.org/public/igem/pSB1AT3/annotation1823801/range1823801/1
type: Range
persistent identity: range1823801
display id: range1823801
version: 1
direction: direction/forward
top level: pSB1AT3
start: 34
end: 35
orientation: inline

ORI
type: SequenceAnnotation
persistent identity: annotation1780315
display id: annotation1780315
version: 1
top level: pSB1AT3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1AT3/annotation1780315/range1780315/1

https://synbiohub.org/public/igem/pSB1AT3/annotation1780315/range1780315/1
type: Range
persistent identity: range1780315
display id: range1780315
version: 1
direction: direction/forward
top level: pSB1AT3
start: 276
end: 276
orientation: inline

Prefix
type: SequenceAnnotation
persistent identity: annotation1780064
display id: annotation1780064
version: 1
top level: pSB1AT3
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB1AT3/annotation1780064/range1780064/1

https://synbiohub.org/public/igem/pSB1AT3/annotation1780064/range1780064/1
type: Range
persistent identity: range1780064
display id: range1780064
version: 1
direction: direction/forward
top level: pSB1AT3
start: 3425
end: 3446
orientation: inline

terminator stem loop
type: SequenceAnnotation
persistent identity: annotation1780328
display id: annotation1780328
version: 1
top level: pSB1AT3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1AT3/annotation1780328/range1780328/1

https://synbiohub.org/public/igem/pSB1AT3/annotation1780328/range1780328/1
type: Range
persistent identity: range1780328
display id: range1780328
version: 1
direction: direction/forward
top level: pSB1AT3
start: 30
end: 64
orientation: inline

end of terminator
type: SequenceAnnotation
persistent identity: annotation1824010
display id: annotation1824010
version: 1
top level: pSB1AT3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1AT3/annotation1824010/range1824010/1

https://synbiohub.org/public/igem/pSB1AT3/annotation1824010/range1824010/1
type: Range
persistent identity: range1824010
display id: range1824010
version: 1
direction: direction/reverse
top level: pSB1AT3
start: 3383
end: 3386
orientation: inline

Tet-r
type: SequenceAnnotation
persistent identity: annotation1780313
display id: annotation1780313
version: 1
top level: pSB1AT3
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB1AT3/annotation1780313/range1780313/1

https://synbiohub.org/public/igem/pSB1AT3/annotation1780313/range1780313/1
type: Range
persistent identity: range1780313
display id: range1780313
version: 1
direction: direction/forward
top level: pSB1AT3
start: 1127
end: 2317
orientation: inline

Suffix
type: SequenceAnnotation
persistent identity: annotation1780046
display id: annotation1780046
version: 1
top level: pSB1AT3
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB1AT3/annotation1780046/range1780046/1

https://synbiohub.org/public/igem/pSB1AT3/annotation1780046/range1780046/1
type: Range
persistent identity: range1780046
display id: range1780046
version: 1
direction: direction/forward
top level: pSB1AT3
start: 1
end: 21
orientation: inline

E. coli his operon terminator
type: SequenceAnnotation
persistent identity: annotation1824009
display id: annotation1824009
version: 1
top level: pSB1AT3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1AT3/annotation1824009/range1824009/1

https://synbiohub.org/public/igem/pSB1AT3/annotation1824009/range1824009/1
type: Range
persistent identity: range1824009
display id: range1824009
version: 1
direction: direction/forward
top level: pSB1AT3
start: 22
end: 93
orientation: inline

rep (pMB1) (rev)
type: SequenceAnnotation
persistent identity: annotation1780066
display id: annotation1780066
version: 1
top level: pSB1AT3
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB1AT3/annotation1780066/range1780066/1

https://synbiohub.org/public/igem/pSB1AT3/annotation1780066/range1780066/1
type: Range
persistent identity: range1780066
display id: range1780066
version: 1
direction: direction/reverse
top level: pSB1AT3
start: 261
end: 875
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation1780316
display id: annotation1780316
version: 1
top level: pSB1AT3
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB1AT3/annotation1780316/range1780316/1

https://synbiohub.org/public/igem/pSB1AT3/annotation1780316/range1780316/1
type: Range
persistent identity: range1780316
display id: range1780316
version: 1
direction: direction/reverse
top level: pSB1AT3
start: 157
end: 176
orientation: inline

APr (rev)
type: SequenceAnnotation
persistent identity: annotation1780314
display id: annotation1780314
version: 1
top level: pSB1AT3
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB1AT3/annotation1780314/range1780314/1

https://synbiohub.org/public/igem/pSB1AT3/annotation1780314/range1780314/1
type: Range
persistent identity: range1780314
display id: range1780314
version: 1
direction: direction/reverse
top level: pSB1AT3
start: 2324
end: 3184
orientation: inline

terminator stem loop
type: SequenceAnnotation
persistent identity: annotation1780318
display id: annotation1780318
version: 1
top level: pSB1AT3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1AT3/annotation1780318/range1780318/1

https://synbiohub.org/public/igem/pSB1AT3/annotation1780318/range1780318/1
type: Range
persistent identity: range1780318
display id: range1780318
version: 1
direction: direction/reverse
top level: pSB1AT3
start: 3396
end: 3415
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation1780317
display id: annotation1780317
version: 1
top level: pSB1AT3
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB1AT3/annotation1780317/range1780317/1

https://synbiohub.org/public/igem/pSB1AT3/annotation1780317/range1780317/1
type: Range
persistent identity: range1780317
display id: range1780317
version: 1
direction: direction/forward
top level: pSB1AT3
start: 3307
end: 3326
orientation: inline

pMam
type: ComponentDefinition
persistent identity: BBa K678023
display id: BBa_K678023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plasmid-Mam for Plug
creator: DTU-Denmark-2
created: 2011-09-04T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Plasmid-Mam for Plug'n'Play
mutable notes: Compatible with the Plug'n'Play assembly standard
mutable provenance: PU0020
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Plasmid Mam
sequence annotation: Linker
sequence: https://synbiohub.org/public/igem/BBa_K678023_sequence/1

Plasmid Mam
type: SequenceAnnotation
persistent identity: annotation2127361
display id: annotation2127361
version: 1
top level: pMam
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678023/annotation2127361/range2127361/1

https://synbiohub.org/public/igem/BBa_K678023/annotation2127361/range2127361/1
type: Range
persistent identity: range2127361
display id: range2127361
version: 1
direction: direction/forward
top level: pMam
start: 9
end: 2711
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2127360
display id: annotation2127360
version: 1
top level: pMam
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678023/annotation2127360/range2127360/1

https://synbiohub.org/public/igem/BBa_K678023/annotation2127360/range2127360/1
type: Range
persistent identity: range2127360
display id: range2127360
version: 1
direction: direction/forward
top level: pMam
start: 1
end: 8
orientation: inline

pSB1AK3
type: ComponentDefinition
persistent identity: pSB1AK3
display id: pSB1AK3
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: High copy BioBrick assembly plasmid
creator: Registry
created: 2004-07-27T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_48_1_
m user id: 0
owner id: 60
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: pSB1AK3 is a high copy number plasmid carrying ampicillin and kanamycin resistance. The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell).
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: deletion of two bases
sequence annotation: rep (pMB1) (rev)
sequence annotation: ampR (rev)
sequence annotation: Suffix
sequence annotation: E. coli his terminator
sequence annotation: terminator stem loop
sequence annotation: VR
sequence annotation: Prefix
sequence annotation: kanR (rev)
sequence annotation: VF2
sequence annotation: end of terminator
sequence annotation: ORI
sequence: https://synbiohub.org/public/igem/pSB1AK3_sequence/1

deletion of two bases
type: SequenceAnnotation
persistent identity: annotation1823805
display id: annotation1823805
version: 1
top level: pSB1AK3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1AK3/annotation1823805/range1823805/1

https://synbiohub.org/public/igem/pSB1AK3/annotation1823805/range1823805/1
type: Range
persistent identity: range1823805
display id: range1823805
version: 1
direction: direction/forward
top level: pSB1AK3
start: 34
end: 35
orientation: inline

rep (pMB1) (rev)
type: SequenceAnnotation
persistent identity: annotation1780429
display id: annotation1780429
version: 1
top level: pSB1AK3
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB1AK3/annotation1780429/range1780429/1

https://synbiohub.org/public/igem/pSB1AK3/annotation1780429/range1780429/1
type: Range
persistent identity: range1780429
display id: range1780429
version: 1
direction: direction/reverse
top level: pSB1AK3
start: 261
end: 875
orientation: inline

ampR (rev)
type: SequenceAnnotation
persistent identity: annotation1780431
display id: annotation1780431
version: 1
top level: pSB1AK3
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB1AK3/annotation1780431/range1780431/1

https://synbiohub.org/public/igem/pSB1AK3/annotation1780431/range1780431/1
type: Range
persistent identity: range1780431
display id: range1780431
version: 1
direction: direction/reverse
top level: pSB1AK3
start: 2067
end: 2927
orientation: inline

Suffix
type: SequenceAnnotation
persistent identity: annotation1780400
display id: annotation1780400
version: 1
top level: pSB1AK3
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB1AK3/annotation1780400/range1780400/1

https://synbiohub.org/public/igem/pSB1AK3/annotation1780400/range1780400/1
type: Range
persistent identity: range1780400
display id: range1780400
version: 1
direction: direction/forward
top level: pSB1AK3
start: 1
end: 21
orientation: inline

E. coli his terminator
type: SequenceAnnotation
persistent identity: annotation1823992
display id: annotation1823992
version: 1
top level: pSB1AK3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1AK3/annotation1823992/range1823992/1

https://synbiohub.org/public/igem/pSB1AK3/annotation1823992/range1823992/1
type: Range
persistent identity: range1823992
display id: range1823992
version: 1
direction: direction/forward
top level: pSB1AK3
start: 22
end: 93
orientation: inline

terminator stem loop
type: SequenceAnnotation
persistent identity: annotation1780433
display id: annotation1780433
version: 1
top level: pSB1AK3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1AK3/annotation1780433/range1780433/1

https://synbiohub.org/public/igem/pSB1AK3/annotation1780433/range1780433/1
type: Range
persistent identity: range1780433
display id: range1780433
version: 1
direction: direction/reverse
top level: pSB1AK3
start: 3139
end: 3158
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation1780436
display id: annotation1780436
version: 1
top level: pSB1AK3
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB1AK3/annotation1780436/range1780436/1

https://synbiohub.org/public/igem/pSB1AK3/annotation1780436/range1780436/1
type: Range
persistent identity: range1780436
display id: range1780436
version: 1
direction: direction/reverse
top level: pSB1AK3
start: 157
end: 176
orientation: inline

Prefix
type: SequenceAnnotation
persistent identity: annotation1780401
display id: annotation1780401
version: 1
top level: pSB1AK3
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB1AK3/annotation1780401/range1780401/1

https://synbiohub.org/public/igem/pSB1AK3/annotation1780401/range1780401/1
type: Range
persistent identity: range1780401
display id: range1780401
version: 1
direction: direction/forward
top level: pSB1AK3
start: 3168
end: 3189
orientation: inline

kanR (rev)
type: SequenceAnnotation
persistent identity: annotation1780430
display id: annotation1780430
version: 1
top level: pSB1AK3
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB1AK3/annotation1780430/range1780430/1

https://synbiohub.org/public/igem/pSB1AK3/annotation1780430/range1780430/1
type: Range
persistent identity: range1780430
display id: range1780430
version: 1
direction: direction/reverse
top level: pSB1AK3
start: 1097
end: 1912
orientation: inline

terminator stem loop
type: SequenceAnnotation
persistent identity: annotation1780434
display id: annotation1780434
version: 1
top level: pSB1AK3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1AK3/annotation1780434/range1780434/1

https://synbiohub.org/public/igem/pSB1AK3/annotation1780434/range1780434/1
type: Range
persistent identity: range1780434
display id: range1780434
version: 1
direction: direction/forward
top level: pSB1AK3
start: 30
end: 64
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation1780435
display id: annotation1780435
version: 1
top level: pSB1AK3
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB1AK3/annotation1780435/range1780435/1

https://synbiohub.org/public/igem/pSB1AK3/annotation1780435/range1780435/1
type: Range
persistent identity: range1780435
display id: range1780435
version: 1
direction: direction/forward
top level: pSB1AK3
start: 3050
end: 3069
orientation: inline

end of terminator
type: SequenceAnnotation
persistent identity: annotation1823993
display id: annotation1823993
version: 1
top level: pSB1AK3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1AK3/annotation1823993/range1823993/1

https://synbiohub.org/public/igem/pSB1AK3/annotation1823993/range1823993/1
type: Range
persistent identity: range1823993
display id: range1823993
version: 1
direction: direction/reverse
top level: pSB1AK3
start: 3126
end: 3129
orientation: inline

ORI
type: SequenceAnnotation
persistent identity: annotation1780432
display id: annotation1780432
version: 1
top level: pSB1AK3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1AK3/annotation1780432/range1780432/1

https://synbiohub.org/public/igem/pSB1AK3/annotation1780432/range1780432/1
type: Range
persistent identity: range1780432
display id: range1780432
version: 1
direction: direction/forward
top level: pSB1AK3
start: 276
end: 276
orientation: inline

BBa_K332033
type: ComponentDefinition
persistent identity: BBa K332033
display id: BBa_K332033
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 37℃ induced RBS + tetR + double terminator
creator: KUO, CHIA-CHUN
created: 2010-10-23T11:00:00Z
modified: 2015-05-08T01:12:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _452_
m user id: 0
owner id: 7059
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: It is a transcriptional unit including 37℃ induced RBS + tetR + double terminator. It can be added various promoter to adapt your conditions.
mutable notes: To regulate following genes by temperature. To maintain cells at 37?? C ↑ : the tetR protein be generated and repress Ptet's circuit. To release cells at 37?? C ↓ : the tetR protein not be generated and do not repress Ptet's circuit.
mutable provenance: the 37℃ induced RBS cames from iGEM08_TUDelft
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_K115002
component: BBa_C0040
component: BBa_J61048
sequence annotation: BBa_K115002
sequence annotation: BBa_J61048
sequence annotation: BBa_C0040
sequence: https://synbiohub.org/public/igem/BBa_K332033_sequence/1

BBa_K115002
type: Component
persistent identity: component2247611
display id: component2247611
version: 1
top level: BBa_K332033
access: public
definition: https://synbiohub.org/public/igem/BBa_K115002/1

BBa_C0040
type: Component
persistent identity: component2247615
display id: component2247615
version: 1
top level: BBa_K332033
access: public
definition: https://synbiohub.org/public/igem/BBa_C0040/1

BBa_J61048
type: Component
persistent identity: component2247616
display id: component2247616
version: 1
top level: BBa_K332033
access: public
definition: https://synbiohub.org/public/igem/BBa_J61048/1

BBa_K115002
type: SequenceAnnotation
persistent identity: annotation2247611
display id: annotation2247611
version: 1
top level: BBa_K332033
location: https://synbiohub.org/public/igem/BBa_K332033/annotation2247611/range2247611/1
component: BBa_K115002

https://synbiohub.org/public/igem/BBa_K332033/annotation2247611/range2247611/1
type: Range
persistent identity: range2247611
display id: range2247611
version: 1
direction: direction/reverse
top level: BBa_K332033
start: 1
end: 52
orientation: inline

BBa_J61048
type: SequenceAnnotation
persistent identity: annotation2247616
display id: annotation2247616
version: 1
top level: BBa_K332033
location: https://synbiohub.org/public/igem/BBa_K332033/annotation2247616/range2247616/1
component: BBa_J61048

https://synbiohub.org/public/igem/BBa_K332033/annotation2247616/range2247616/1
type: Range
persistent identity: range2247616
display id: range2247616
version: 1
direction: direction/reverse
top level: BBa_K332033
start: 752
end: 864
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation2247615
display id: annotation2247615
version: 1
top level: BBa_K332033
location: https://synbiohub.org/public/igem/BBa_K332033/annotation2247615/range2247615/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_K332033/annotation2247615/range2247615/1
type: Range
persistent identity: range2247615
display id: range2247615
version: 1
direction: direction/reverse
top level: BBa_K332033
start: 59
end: 743
orientation: inline

pSB1A7
type: ComponentDefinition
persistent identity: pSB1A7
display id: pSB1A7
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Transcriptionally insulated high copy BioBrick plasmid
creator: Karmella Haynes
created: 2006-10-18T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _61_
m user id: 0
owner id: 1144
owning group id: 61
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: pSB1A4 was constructed by adding double terminators ([BBa_B0015]) before the BioBrick prefix and after the BioBrick suffix in pSB1A3. This insulator blocks read-through transcription into BioBrick parts/ devices.
mutable notes: The double terminators were assembled from small smaller overlapping ssDNA oligos. The outer-most EcoRI and PstI sites were mutated while the inner-most BioBrick cut sites were restored.
mutable provenance: Based upon pSB1A3; Includes two copies of [BBa_B0015] assembled from DNA oligos
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: stem_loop
sequence annotation: B0015 (rev)
sequence annotation: B0015
sequence annotation: end of terminator
sequence annotation: mutated EcoRI
sequence annotation: ampR (rev)
sequence annotation: VR
sequence annotation: suffix
sequence annotation: rep(pMB1) (rev)
sequence annotation: prefix
sequence annotation: c > g
sequence annotation: VF2
sequence annotation: E. coli his operon terminator
sequence annotation: mutated PstI
sequence: https://synbiohub.org/public/igem/pSB1A7_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation1903646
display id: annotation1903646
version: 1
top level: pSB1A7
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1A7/annotation1903646/range1903646/1

https://synbiohub.org/public/igem/pSB1A7/annotation1903646/range1903646/1
type: Range
persistent identity: range1903646
display id: range1903646
version: 1
direction: direction/reverse
top level: pSB1A7
start: 98
end: 141
orientation: inline

B0015 (rev)
type: SequenceAnnotation
persistent identity: annotation1903644
display id: annotation1903644
version: 1
top level: pSB1A7
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB1A7/annotation1903644/range1903644/1

https://synbiohub.org/public/igem/pSB1A7/annotation1903644/range1903644/1
type: Range
persistent identity: range1903644
display id: range1903644
version: 1
direction: direction/reverse
top level: pSB1A7
start: 24
end: 152
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation1903658
display id: annotation1903658
version: 1
top level: pSB1A7
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB1A7/annotation1903658/range1903658/1

https://synbiohub.org/public/igem/pSB1A7/annotation1903658/range1903658/1
type: Range
persistent identity: range1903658
display id: range1903658
version: 1
direction: direction/forward
top level: pSB1A7
start: 2279
end: 2407
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1903645
display id: annotation1903645
version: 1
top level: pSB1A7
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1A7/annotation1903645/range1903645/1

https://synbiohub.org/public/igem/pSB1A7/annotation1903645/range1903645/1
type: Range
persistent identity: range1903645
display id: range1903645
version: 1
direction: direction/reverse
top level: pSB1A7
start: 38
end: 57
orientation: inline

end of terminator
type: SequenceAnnotation
persistent identity: annotation1903654
display id: annotation1903654
version: 1
top level: pSB1A7
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1A7/annotation1903654/range1903654/1

https://synbiohub.org/public/igem/pSB1A7/annotation1903654/range1903654/1
type: Range
persistent identity: range1903654
display id: range1903654
version: 1
direction: direction/forward
top level: pSB1A7
start: 2231
end: 2234
orientation: inline

mutated EcoRI
type: SequenceAnnotation
persistent identity: annotation1903656
display id: annotation1903656
version: 1
top level: pSB1A7
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1A7/annotation1903656/range1903656/1

https://synbiohub.org/public/igem/pSB1A7/annotation1903656/range1903656/1
type: Range
persistent identity: range1903656
display id: range1903656
version: 1
direction: direction/forward
top level: pSB1A7
start: 2273
end: 2278
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1903650
display id: annotation1903650
version: 1
top level: pSB1A7
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1A7/annotation1903650/range1903650/1

https://synbiohub.org/public/igem/pSB1A7/annotation1903650/range1903650/1
type: Range
persistent identity: range1903650
display id: range1903650
version: 1
direction: direction/forward
top level: pSB1A7
start: 167
end: 201
orientation: inline

ampR (rev)
type: SequenceAnnotation
persistent identity: annotation1903653
display id: annotation1903653
version: 1
top level: pSB1A7
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB1A7/annotation1903653/range1903653/1

https://synbiohub.org/public/igem/pSB1A7/annotation1903653/range1903653/1
type: Range
persistent identity: range1903653
display id: range1903653
version: 1
direction: direction/reverse
top level: pSB1A7
start: 1172
end: 2032
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation1903651
display id: annotation1903651
version: 1
top level: pSB1A7
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB1A7/annotation1903651/range1903651/1

https://synbiohub.org/public/igem/pSB1A7/annotation1903651/range1903651/1
type: Range
persistent identity: range1903651
display id: range1903651
version: 1
direction: direction/reverse
top level: pSB1A7
start: 294
end: 313
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1903659
display id: annotation1903659
version: 1
top level: pSB1A7
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1A7/annotation1903659/range1903659/1

https://synbiohub.org/public/igem/pSB1A7/annotation1903659/range1903659/1
type: Range
persistent identity: range1903659
display id: range1903659
version: 1
direction: direction/forward
top level: pSB1A7
start: 2290
end: 2333
orientation: inline

suffix
type: SequenceAnnotation
persistent identity: annotation1903642
display id: annotation1903642
version: 1
top level: pSB1A7
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB1A7/annotation1903642/range1903642/1

https://synbiohub.org/public/igem/pSB1A7/annotation1903642/range1903642/1
type: Range
persistent identity: range1903642
display id: range1903642
version: 1
direction: direction/forward
top level: pSB1A7
start: 1
end: 20
orientation: inline

rep(pMB1) (rev)
type: SequenceAnnotation
persistent identity: annotation1903652
display id: annotation1903652
version: 1
top level: pSB1A7
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB1A7/annotation1903652/range1903652/1

https://synbiohub.org/public/igem/pSB1A7/annotation1903652/range1903652/1
type: Range
persistent identity: range1903652
display id: range1903652
version: 1
direction: direction/reverse
top level: pSB1A7
start: 398
end: 1012
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation1903661
display id: annotation1903661
version: 1
top level: pSB1A7
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB1A7/annotation1903661/range1903661/1

https://synbiohub.org/public/igem/pSB1A7/annotation1903661/range1903661/1
type: Range
persistent identity: range1903661
display id: range1903661
version: 1
direction: direction/forward
top level: pSB1A7
start: 2411
end: 2431
orientation: inline

c > g
type: SequenceAnnotation
persistent identity: annotation1903647
display id: annotation1903647
version: 1
top level: pSB1A7
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB1A7/annotation1903647/range1903647/1

https://synbiohub.org/public/igem/pSB1A7/annotation1903647/range1903647/1
type: Range
persistent identity: range1903647
display id: range1903647
version: 1
direction: direction/forward
top level: pSB1A7
start: 153
end: 153
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation1903643
display id: annotation1903643
version: 1
top level: pSB1A7
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB1A7/annotation1903643/range1903643/1

https://synbiohub.org/public/igem/pSB1A7/annotation1903643/range1903643/1
type: Range
persistent identity: range1903643
display id: range1903643
version: 1
direction: direction/forward
top level: pSB1A7
start: 2155
end: 2174
orientation: inline

E. coli his operon terminator
type: SequenceAnnotation
persistent identity: annotation1903649
display id: annotation1903649
version: 1
top level: pSB1A7
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1A7/annotation1903649/range1903649/1

https://synbiohub.org/public/igem/pSB1A7/annotation1903649/range1903649/1
type: Range
persistent identity: range1903649
display id: range1903649
version: 1
direction: direction/forward
top level: pSB1A7
start: 159
end: 229
orientation: inline

mutated PstI
type: SequenceAnnotation
persistent identity: annotation1903648
display id: annotation1903648
version: 1
top level: pSB1A7
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1A7/annotation1903648/range1903648/1

https://synbiohub.org/public/igem/pSB1A7/annotation1903648/range1903648/1
type: Range
persistent identity: range1903648
display id: range1903648
version: 1
direction: direction/forward
top level: pSB1A7
start: 153
end: 158
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1903655
display id: annotation1903655
version: 1
top level: pSB1A7
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1A7/annotation1903655/range1903655/1

https://synbiohub.org/public/igem/pSB1A7/annotation1903655/range1903655/1
type: Range
persistent identity: range1903655
display id: range1903655
version: 1
direction: direction/forward
top level: pSB1A7
start: 2244
end: 2263
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1903660
display id: annotation1903660
version: 1
top level: pSB1A7
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1A7/annotation1903660/range1903660/1

https://synbiohub.org/public/igem/pSB1A7/annotation1903660/range1903660/1
type: Range
persistent identity: range1903660
display id: range1903660
version: 1
direction: direction/forward
top level: pSB1A7
start: 2374
end: 2393
orientation: inline

c > g
type: SequenceAnnotation
persistent identity: annotation1903657
display id: annotation1903657
version: 1
top level: pSB1A7
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB1A7/annotation1903657/range1903657/1

https://synbiohub.org/public/igem/pSB1A7/annotation1903657/range1903657/1
type: Range
persistent identity: range1903657
display id: range1903657
version: 1
direction: direction/forward
top level: pSB1A7
start: 2278
end: 2278
orientation: inline

Screening
type: ComponentDefinition
persistent identity: pSB1A10
display id: pSB1A10
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Screening Plasmid v1.0
creator: Josh Michener, Jason Kelly
created: 2006-05-25T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_
m user id: 0
owner id: 2
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Scar from BB suffix removal by I13450
sequence annotation: I0500 (pBAD/araC)
sequence annotation: prefix
sequence annotation: B0034
sequence annotation: B0015 (Term)
sequence annotation: Hairpin
sequence annotation: RNAse E site
sequence annotation: Scar from BB prefix removal by I13450
sequence annotation: spacer artifact from 13450
sequence annotation: E0040 (EGFP)
sequence annotation: psb1a3
sequence annotation: barcode
sequence annotation: suffix
sequence annotation: E1010 (mRFP1)
sequence: https://synbiohub.org/public/igem/pSB1A10_sequence/1

Scar from BB suffix removal by I13450
type: SequenceAnnotation
persistent identity: annotation1871312
display id: annotation1871312
version: 1
top level: Screening
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1A10/annotation1871312/range1871312/1

https://synbiohub.org/public/igem/pSB1A10/annotation1871312/range1871312/1
type: Range
persistent identity: range1871312
display id: range1871312
version: 1
direction: direction/forward
top level: Screening
start: 963
end: 1044
orientation: inline

I0500 (pBAD/araC)
type: SequenceAnnotation
persistent identity: annotation1871315
display id: annotation1871315
version: 1
top level: Screening
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/pSB1A10/annotation1871315/range1871315/1

https://synbiohub.org/public/igem/pSB1A10/annotation1871315/range1871315/1
type: Range
persistent identity: range1871315
display id: range1871315
version: 1
direction: direction/forward
top level: Screening
start: 3239
end: 4448
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation1871320
display id: annotation1871320
version: 1
top level: Screening
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB1A10/annotation1871320/range1871320/1

https://synbiohub.org/public/igem/pSB1A10/annotation1871320/range1871320/1
type: Range
persistent identity: range1871320
display id: range1871320
version: 1
direction: direction/forward
top level: Screening
start: 5242
end: 5263
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation1871308
display id: annotation1871308
version: 1
top level: Screening
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/pSB1A10/annotation1871308/range1871308/1

https://synbiohub.org/public/igem/pSB1A10/annotation1871308/range1871308/1
type: Range
persistent identity: range1871308
display id: range1871308
version: 1
direction: direction/forward
top level: Screening
start: 102
end: 113
orientation: inline

B0015 (Term)
type: SequenceAnnotation
persistent identity: annotation1871311
display id: annotation1871311
version: 1
top level: Screening
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1A10/annotation1871311/range1871311/1

https://synbiohub.org/public/igem/pSB1A10/annotation1871311/range1871311/1
type: Range
persistent identity: range1871311
display id: range1871311
version: 1
direction: direction/forward
top level: Screening
start: 834
end: 962
orientation: inline

Hairpin
type: SequenceAnnotation
persistent identity: annotation1871307
display id: annotation1871307
version: 1
top level: Screening
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1A10/annotation1871307/range1871307/1

https://synbiohub.org/public/igem/pSB1A10/annotation1871307/range1871307/1
type: Range
persistent identity: range1871307
display id: range1871307
version: 1
direction: direction/forward
top level: Screening
start: 59
end: 93
orientation: inline

RNAse E site
type: SequenceAnnotation
persistent identity: annotation1871306
display id: annotation1871306
version: 1
top level: Screening
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1A10/annotation1871306/range1871306/1

https://synbiohub.org/public/igem/pSB1A10/annotation1871306/range1871306/1
type: Range
persistent identity: range1871306
display id: range1871306
version: 1
direction: direction/forward
top level: Screening
start: 38
end: 58
orientation: inline

RNAse E site
type: SequenceAnnotation
persistent identity: annotation1871318
display id: annotation1871318
version: 1
top level: Screening
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1A10/annotation1871318/range1871318/1

https://synbiohub.org/public/igem/pSB1A10/annotation1871318/range1871318/1
type: Range
persistent identity: range1871318
display id: range1871318
version: 1
direction: direction/forward
top level: Screening
start: 5203
end: 5223
orientation: inline

Scar from BB prefix removal by I13450
type: SequenceAnnotation
persistent identity: annotation1871314
display id: annotation1871314
version: 1
top level: Screening
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1A10/annotation1871314/range1871314/1

https://synbiohub.org/public/igem/pSB1A10/annotation1871314/range1871314/1
type: Range
persistent identity: range1871314
display id: range1871314
version: 1
direction: direction/forward
top level: Screening
start: 3158
end: 3238
orientation: inline

spacer artifact from 13450
type: SequenceAnnotation
persistent identity: annotation1871305
display id: annotation1871305
version: 1
top level: Screening
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1A10/annotation1871305/range1871305/1

https://synbiohub.org/public/igem/pSB1A10/annotation1871305/range1871305/1
type: Range
persistent identity: range1871305
display id: range1871305
version: 1
direction: direction/forward
top level: Screening
start: 22
end: 37
orientation: inline

E0040 (EGFP)
type: SequenceAnnotation
persistent identity: annotation1871317
display id: annotation1871317
version: 1
top level: Screening
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB1A10/annotation1871317/range1871317/1

https://synbiohub.org/public/igem/pSB1A10/annotation1871317/range1871317/1
type: Range
persistent identity: range1871317
display id: range1871317
version: 1
direction: direction/forward
top level: Screening
start: 4475
end: 5194
orientation: inline

psb1a3
type: SequenceAnnotation
persistent identity: annotation1871313
display id: annotation1871313
version: 1
top level: Screening
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1A10/annotation1871313/range1871313/1

https://synbiohub.org/public/igem/pSB1A10/annotation1871313/range1871313/1
type: Range
persistent identity: range1871313
display id: range1871313
version: 1
direction: direction/forward
top level: Screening
start: 1045
end: 3157
orientation: inline

barcode
type: SequenceAnnotation
persistent identity: annotation1871310
display id: annotation1871310
version: 1
top level: Screening
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/pSB1A10/annotation1871310/range1871310/1

https://synbiohub.org/public/igem/pSB1A10/annotation1871310/range1871310/1
type: Range
persistent identity: range1871310
display id: range1871310
version: 1
direction: direction/forward
top level: Screening
start: 801
end: 825
orientation: inline

suffix
type: SequenceAnnotation
persistent identity: annotation1871283
display id: annotation1871283
version: 1
top level: Screening
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB1A10/annotation1871283/range1871283/1

https://synbiohub.org/public/igem/pSB1A10/annotation1871283/range1871283/1
type: Range
persistent identity: range1871283
display id: range1871283
version: 1
direction: direction/forward
top level: Screening
start: 1
end: 21
orientation: inline

spacer artifact from 13450
type: SequenceAnnotation
persistent identity: annotation1871319
display id: annotation1871319
version: 1
top level: Screening
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1A10/annotation1871319/range1871319/1

https://synbiohub.org/public/igem/pSB1A10/annotation1871319/range1871319/1
type: Range
persistent identity: range1871319
display id: range1871319
version: 1
direction: direction/forward
top level: Screening
start: 5224
end: 5241
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation1871316
display id: annotation1871316
version: 1
top level: Screening
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/pSB1A10/annotation1871316/range1871316/1

https://synbiohub.org/public/igem/pSB1A10/annotation1871316/range1871316/1
type: Range
persistent identity: range1871316
display id: range1871316
version: 1
direction: direction/forward
top level: Screening
start: 4457
end: 4468
orientation: inline

E1010 (mRFP1)
type: SequenceAnnotation
persistent identity: annotation1871309
display id: annotation1871309
version: 1
top level: Screening
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB1A10/annotation1871309/range1871309/1

https://synbiohub.org/public/igem/pSB1A10/annotation1871309/range1871309/1
type: Range
persistent identity: range1871309
display id: range1871309
version: 1
direction: direction/forward
top level: Screening
start: 120
end: 800
orientation: inline

BBa_K332031
type: ComponentDefinition
persistent identity: BBa K332031
display id: BBa_K332031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutive promoter + 37℃ induced RBS + tetR + double terminator
creator: KUO, CHIA-CHUN
created: 2010-10-21T11:00:00Z
modified: 2015-05-08T01:12:05Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _452_
m user id: 0
owner id: 7059
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: we took advantage of the interaction between 37?? C induced RBS, tetR gene and pTet promoter to create the temperature regulating circuit. The following is a description of our prescribed strategy. First, we use the constitutive promoter, 37?? C induced RBS (K115002), the tetR gene and terminators. We will call this part the ???Regulator???. We speculate that when the ???Regulator??? and ???Production??? sections are combined, we can achieve a high bacteria growth rate and close to no target protein producing rate at T>37??C, and low bacteria growth rate and high target protein producing rate at T<37??C.
mutable notes: To regulate following gene by temperature
mutable provenance: iGEM08_TUDelft
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_C0040
component: BBa_K115002
component: BBa_J23101
component: BBa_J61048
sequence annotation: BBa_J23101
sequence annotation: BBa_C0040
sequence annotation: BBa_J61048
sequence annotation: BBa_K115002
sequence: https://synbiohub.org/public/igem/BBa_K332031_sequence/1

BBa_C0040
type: Component
persistent identity: component2250406
display id: component2250406
version: 1
top level: BBa_K332031
access: public
definition: https://synbiohub.org/public/igem/BBa_C0040/1

BBa_K115002
type: Component
persistent identity: component2250402
display id: component2250402
version: 1
top level: BBa_K332031
access: public
definition: https://synbiohub.org/public/igem/BBa_K115002/1

BBa_J23101
type: Component
persistent identity: component2250397
display id: component2250397
version: 1
top level: BBa_K332031
access: public
definition: https://synbiohub.org/public/igem/BBa_J23101/1

BBa_J61048
type: Component
persistent identity: component2250407
display id: component2250407
version: 1
top level: BBa_K332031
access: public
definition: https://synbiohub.org/public/igem/BBa_J61048/1

BBa_J23101
type: SequenceAnnotation
persistent identity: annotation2250397
display id: annotation2250397
version: 1
top level: BBa_K332031
location: https://synbiohub.org/public/igem/BBa_K332031/annotation2250397/range2250397/1
component: BBa_J23101

https://synbiohub.org/public/igem/BBa_K332031/annotation2250397/range2250397/1
type: Range
persistent identity: range2250397
display id: range2250397
version: 1
direction: direction/reverse
top level: BBa_K332031
start: 1
end: 35
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation2250406
display id: annotation2250406
version: 1
top level: BBa_K332031
location: https://synbiohub.org/public/igem/BBa_K332031/annotation2250406/range2250406/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_K332031/annotation2250406/range2250406/1
type: Range
persistent identity: range2250406
display id: range2250406
version: 1
direction: direction/reverse
top level: BBa_K332031
start: 102
end: 786
orientation: inline

BBa_J61048
type: SequenceAnnotation
persistent identity: annotation2250407
display id: annotation2250407
version: 1
top level: BBa_K332031
location: https://synbiohub.org/public/igem/BBa_K332031/annotation2250407/range2250407/1
component: BBa_J61048

https://synbiohub.org/public/igem/BBa_K332031/annotation2250407/range2250407/1
type: Range
persistent identity: range2250407
display id: range2250407
version: 1
direction: direction/reverse
top level: BBa_K332031
start: 795
end: 907
orientation: inline

BBa_K115002
type: SequenceAnnotation
persistent identity: annotation2250402
display id: annotation2250402
version: 1
top level: BBa_K332031
location: https://synbiohub.org/public/igem/BBa_K332031/annotation2250402/range2250402/1
component: BBa_K115002

https://synbiohub.org/public/igem/BBa_K332031/annotation2250402/range2250402/1
type: Range
persistent identity: range2250402
display id: range2250402
version: 1
direction: direction/reverse
top level: BBa_K332031
start: 44
end: 95
orientation: inline

BBa_K801002
type: ComponentDefinition
persistent identity: BBa K801002
display id: BBa_K801002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pTUM102 yeast shuttle vector with pTEF2 promoter
creator: Georg Sch??tzinger
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1057_
m user id: 0
owner id: 13624
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part corresponds to pTUM100 (BBa_K801000). The constitutive pTEF1 promoter has been cloned in front of the MCS (RFC25 prefix and suffix).
mutable notes: All forbidden restriction sites have been removed from the plasmid backbone, making it RFC25 compatible.
mutable provenance: Based on invitrogen's pYES2 plasmid. For mor information see the part information of pTUM100 (BBa_K801000).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: pUC-origin
sequence annotation: 2 micron (??) origin
sequence annotation: TEF2-Promoter
sequence annotation: CYC1-T
sequence annotation: T7-Promoter
sequence annotation: URA3
sequence annotation: AmpR
sequence: https://synbiohub.org/public/igem/BBa_K801002_sequence/1

pUC-origin
type: SequenceAnnotation
persistent identity: annotation2201446
display id: annotation2201446
version: 1
top level: BBa_K801002
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K801002/annotation2201446/range2201446/1

https://synbiohub.org/public/igem/BBa_K801002/annotation2201446/range2201446/1
type: Range
persistent identity: range2201446
display id: range2201446
version: 1
direction: direction/forward
top level: BBa_K801002
start: 1169
end: 1842
orientation: inline

2 micron (??) origin
type: SequenceAnnotation
persistent identity: annotation2201452
display id: annotation2201452
version: 1
top level: BBa_K801002
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K801002/annotation2201452/range2201452/1

https://synbiohub.org/public/igem/BBa_K801002/annotation2201452/range2201452/1
type: Range
persistent identity: range2201452
display id: range2201452
version: 1
direction: direction/reverse
top level: BBa_K801002
start: 3976
end: 5447
orientation: inline

TEF2-Promoter
type: SequenceAnnotation
persistent identity: annotation2201314
display id: annotation2201314
version: 1
top level: BBa_K801002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K801002/annotation2201314/range2201314/1

https://synbiohub.org/public/igem/BBa_K801002/annotation2201314/range2201314/1
type: Range
persistent identity: range2201314
display id: range2201314
version: 1
direction: direction/forward
top level: BBa_K801002
start: 58
end: 705
orientation: inline

CYC1-T
type: SequenceAnnotation
persistent identity: annotation2201315
display id: annotation2201315
version: 1
top level: BBa_K801002
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K801002/annotation2201315/range2201315/1

https://synbiohub.org/public/igem/BBa_K801002/annotation2201315/range2201315/1
type: Range
persistent identity: range2201315
display id: range2201315
version: 1
direction: direction/forward
top level: BBa_K801002
start: 739
end: 984
orientation: inline

T7-Promoter
type: SequenceAnnotation
persistent identity: annotation2201456
display id: annotation2201456
version: 1
top level: BBa_K801002
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K801002/annotation2201456/range2201456/1

https://synbiohub.org/public/igem/BBa_K801002/annotation2201456/range2201456/1
type: Range
persistent identity: range2201456
display id: range2201456
version: 1
direction: direction/forward
top level: BBa_K801002
start: 4
end: 23
orientation: inline

URA3
type: SequenceAnnotation
persistent identity: annotation2201448
display id: annotation2201448
version: 1
top level: BBa_K801002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801002/annotation2201448/range2201448/1

https://synbiohub.org/public/igem/BBa_K801002/annotation2201448/range2201448/1
type: Range
persistent identity: range2201448
display id: range2201448
version: 1
direction: direction/reverse
top level: BBa_K801002
start: 2865
end: 3972
orientation: inline

AmpR
type: SequenceAnnotation
persistent identity: annotation2201447
display id: annotation2201447
version: 1
top level: BBa_K801002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K801002/annotation2201447/range2201447/1

https://synbiohub.org/public/igem/BBa_K801002/annotation2201447/range2201447/1
type: Range
persistent identity: range2201447
display id: range2201447
version: 1
direction: direction/reverse
top level: BBa_K801002
start: 1987
end: 2846
orientation: inline

cry11Aa
type: ComponentDefinition
persistent identity: BBa K332011
display id: BBa_K332011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Crystal protein gene (cry11Aa) from Bacillus thuringiensis subsp. Israelensis.
creator: Chia-Le Meng
created: 2010-10-23T11:00:00Z
modified: 2015-05-08T01:12:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _452_
m user id: 0
owner id: 7487
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: The cry weapon system produce crystal protein, targetting the wrigglers, larvae of mosquitoes. The cry11Aa gene is cloned from Bacillus thuringiensis subsp. Israelensis. The cry protein is controlled by the tetR-repressible promoter PtetR (BBa_R0040), which in turn is regulated by a temperature control system. When E.coli is released into environment (<37C), tetR begins to degrade, resulting in the promoter PtetR (BBa_R0011) expressing downstream genes, cry11Aa (BBa_K332012) and Green Fluorescent Protein (GFP)(BBa_E0040).
mutable notes: Procedures (I) Culture Bti. Bacillus thuringiensis subsp. Israelensis (BCRC15860) 1. Prepare agar plate for Bti. Beef extract 3.0g Peptone 5.0g Agar 15.0g ddH2O 1 L *adjust PH to 7.0 *No antibiotic *Be aware of contamination 2. Plate Bti. on the medium and incubate the cultures at 30??C overnight. (II) Clone cry11Aa from Bti. into TA vector 1. Extract genomic DNA of Bti. by liquid nitrogen. 2. Add some ddH2O to dilute the DNA. 3. Design primers Vf2: ATTCAATAAAAGGTGGAATGAATTATGGA Tm: 53??C VR: GTGCTAACATGACTTCTACTTTAGT Tm: 52.8??C 4. Find the best PCR condition by gradient PCR. Anneling temperature: 51??C??10??C 5. PCR by B-taq plus on the best condition Template DNA（10ng/μl） 2.0 B-taq buffer 5.0 dNTP（2mM） 5.0 forward primer（10μM） 1.5 reverse primer（10μM） 1.5 B-taq plus DNA polymerase（2Kb） 1.0 ddH2O 34.0 Total 50 μl 6. Digestion to confirm the cry11Aa fragment and enzyme sites. 7. TA clone 8. DNA sequencing (III) PCR mutagenesis at two enzyme sites --- EcoR1 and Spe1 1. Design primers by primerX. EcoR1-Vf2: CGG GTA CAA TCT CAG AAC TCG GGA AAT AAT AGA A EcoR1-VR: TTC TAT TAT TTC CCG AGT TCT GAG ATT GTA CCC G Spe1-Vf2: ATA ATG AAT GGG GAG GAC TGG TTT ATA AGT TAT TAA TGG G Spe1-VR: CTT CCC CCA TTA ATA ACT TAT AAA CTA GTC CTC CCC ATT CAT 2. Digestion to confirm the fragments (IV) PCR construction of Biobrick parts 1. Design primers by Assembly standard 10. Vf2: GTTTCTTC GAATTC GCGGCCGC T TCTAG ATGGAAGATAGTTCTTTAGATACT VR: GTTTCTTC CTGCAG CGGCCGC T ACTAGT A CTACTTTAGTAACGGATT 2. PCR condition 3. Ligation to backbones(Psb1C3). (V) Transform into E.coli 1. Thaw competent cells and BBa_K332012 plasmid on ice. 2. Add 2ul plasmid to competent cell and place in ice for 5 minutes. 3. Put the transformed cells into 42℃ water bath for 45 seconds. 4. Plate the cells on the appropriate media and antibiotic, such as agar plates with 25 ??g/ml kanamycin. 5. Incubate the cultures at 37??C overnight. (VI) Culture with mosquito larvae and observe
mutable provenance: Crystal protein gene (cry11Aa) from Bacillus thuringiensis subsp. Israelensis. BCRC Number: 15860 Organism: Bacillus thuringiensis subsp. israelensis de Barjac History: << ATCC << USDA << ONR 60 << H. de Barjac << L. J. Goldberg 60 Other Collection No.: ATCC 35646 Source: sewage, Israel Characterization: Production of delta-endotoxin against dipteran larvae --B3009 Others: referred to as the type strain --B3008 Growth Conditions: 30??C , Medium ID: 3 Oxygen Requirement: Aerobic Biosafety Level: 1 References: Temeyer, K. B. 1984. Larvicidal activity of Bacillus thuringiensis subsp. israelensis in the dipteran Haematobia irritans. Appl. Environ. Microbiol.47 : 952-955. PMID: 6742837. Demezas, D. H. and J. Bell. 1995. Evaluation of low molecular weight RNA profiles and ribotyping to differentiate some Bacillus species. Syst. Appl. Microbiol.18 : 582-589.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K332011_sequence/1

BBa_K598000
type: ComponentDefinition
persistent identity: BBa K598000
display id: BBa_K598000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TPP Down-regulated Hammerhead Ribozyme 2.5 with Native RBS+E0040+B0015
creator: Yangyang ZHAO, Yan GONG, Zhenrun ZHANG, Qingyang XIAO
created: 2011-09-17T11:00:00Z
modified: 2015-05-08T01:12:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _770_
m user id: 0
owner id: 4411
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This part is TPP up-regulated hammerhead ribozyme, consisting of 131 nucleotides.
mutable notes: This part contains a native ribosomal binding site
mutable provenance: This part was an engineered RNA controller composed of a TPP aptamer from B.subtilis and a hammerhead motif.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: native RBS
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: stop
sequence annotation: stem_loop
sequence annotation: TPP Ribozyme 2.5
sequence annotation: BBa_E0040
sequence annotation: T7 TE
sequence annotation: eGFP
sequence annotation: polya
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K598000_sequence/1

native RBS
type: SequenceAnnotation
persistent identity: annotation2131876
display id: annotation2131876
version: 1
top level: BBa_K598000
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598000/annotation2131876/range2131876/1

https://synbiohub.org/public/igem/BBa_K598000/annotation2131876/range2131876/1
type: Range
persistent identity: range2131876
display id: range2131876
version: 1
direction: direction/forward
top level: BBa_K598000
start: 138
end: 145
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2152836
display id: annotation2152836
version: 1
top level: BBa_K598000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K598000/annotation2152836/range2152836/1

https://synbiohub.org/public/igem/BBa_K598000/annotation2152836/range2152836/1
type: Range
persistent identity: range2152836
display id: range2152836
version: 1
direction: direction/forward
top level: BBa_K598000
start: 879
end: 958
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2152867
display id: annotation2152867
version: 1
top level: BBa_K598000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K598000/annotation2152867/range2152867/1

https://synbiohub.org/public/igem/BBa_K598000/annotation2152867/range2152867/1
type: Range
persistent identity: range2152867
display id: range2152867
version: 1
direction: direction/forward
top level: BBa_K598000
start: 967
end: 1007
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2152872
display id: annotation2152872
version: 1
top level: BBa_K598000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K598000/annotation2152872/range2152872/1

https://synbiohub.org/public/igem/BBa_K598000/annotation2152872/range2152872/1
type: Range
persistent identity: range2152872
display id: range2152872
version: 1
direction: direction/forward
top level: BBa_K598000
start: 1000
end: 1000
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2152857
display id: annotation2152857
version: 1
top level: BBa_K598000
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K598000/annotation2152857/range2152857/1

https://synbiohub.org/public/igem/BBa_K598000/annotation2152857/range2152857/1
type: Range
persistent identity: range2152857
display id: range2152857
version: 1
direction: direction/forward
top level: BBa_K598000
start: 890
end: 933
orientation: inline

TPP Ribozyme 2.5
type: SequenceAnnotation
persistent identity: annotation2152875
display id: annotation2152875
version: 1
top level: BBa_K598000
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K598000/annotation2152875/range2152875/1

https://synbiohub.org/public/igem/BBa_K598000/annotation2152875/range2152875/1
type: Range
persistent identity: range2152875
display id: range2152875
version: 1
direction: direction/forward
top level: BBa_K598000
start: 1
end: 150
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2152880
display id: annotation2152880
version: 1
top level: BBa_K598000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K598000/annotation2152880/range2152880/1

https://synbiohub.org/public/igem/BBa_K598000/annotation2152880/range2152880/1
type: Range
persistent identity: range2152880
display id: range2152880
version: 1
direction: direction/forward
top level: BBa_K598000
start: 151
end: 870
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2152868
display id: annotation2152868
version: 1
top level: BBa_K598000
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K598000/annotation2152868/range2152868/1

https://synbiohub.org/public/igem/BBa_K598000/annotation2152868/range2152868/1
type: Range
persistent identity: range2152868
display id: range2152868
version: 1
direction: direction/forward
top level: BBa_K598000
start: 974
end: 993
orientation: inline

eGFP
type: SequenceAnnotation
persistent identity: annotation2152835
display id: annotation2152835
version: 1
top level: BBa_K598000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K598000/annotation2152835/range2152835/1

https://synbiohub.org/public/igem/BBa_K598000/annotation2152835/range2152835/1
type: Range
persistent identity: range2152835
display id: range2152835
version: 1
direction: direction/forward
top level: BBa_K598000
start: 151
end: 870
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation2152870
display id: annotation2152870
version: 1
top level: BBa_K598000
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K598000/annotation2152870/range2152870/1

https://synbiohub.org/public/igem/BBa_K598000/annotation2152870/range2152870/1
type: Range
persistent identity: range2152870
display id: range2152870
version: 1
direction: direction/forward
top level: BBa_K598000
start: 994
end: 1007
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2153033
display id: annotation2153033
version: 1
top level: BBa_K598000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K598000/annotation2153033/range2153033/1

https://synbiohub.org/public/igem/BBa_K598000/annotation2153033/range2153033/1
type: Range
persistent identity: range2153033
display id: range2153033
version: 1
direction: direction/forward
top level: BBa_K598000
start: 879
end: 1007
orientation: inline

BBa_K598014
type: ComponentDefinition
persistent identity: BBa K598014
display id: BBa_K598014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBAD+HH+CdIntron+E0040+b0015
creator: Xiaowei YAN, Tong MU
created: 2011-09-26T11:00:00Z
modified: 2015-05-08T01:12:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _770_
m user id: 0
owner id: 8713
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This a GFP reporter regulated by a self-cleaving hammerhead ribozyme. The self-cleaving of the hammerhead ribozyme would lead to the formation of the intron P1 stem, thus triggering the self-splicing of the intron.
mutable notes: The self-cleaving of the hammerhead ribozyme would lead to the formation of the intron P1 stem, thus triggering the self-splicing of the intron.
mutable provenance: The intron derives from the genome of Cd
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: HH
sequence annotation: RBS
sequence annotation: eGFP
sequence annotation: BBa_B0015
sequence annotation: pBAD
sequence annotation: group I intron
sequence: https://synbiohub.org/public/igem/BBa_K598014_sequence/1

HH
type: SequenceAnnotation
persistent identity: annotation2153082
display id: annotation2153082
version: 1
top level: BBa_K598014
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K598014/annotation2153082/range2153082/1

https://synbiohub.org/public/igem/BBa_K598014/annotation2153082/range2153082/1
type: Range
persistent identity: range2153082
display id: range2153082
version: 1
direction: direction/forward
top level: BBa_K598014
start: 139
end: 185
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2153088
display id: annotation2153088
version: 1
top level: BBa_K598014
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598014/annotation2153088/range2153088/1

https://synbiohub.org/public/igem/BBa_K598014/annotation2153088/range2153088/1
type: Range
persistent identity: range2153088
display id: range2153088
version: 1
direction: direction/forward
top level: BBa_K598014
start: 187
end: 191
orientation: inline

eGFP
type: SequenceAnnotation
persistent identity: annotation2153086
display id: annotation2153086
version: 1
top level: BBa_K598014
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K598014/annotation2153086/range2153086/1

https://synbiohub.org/public/igem/BBa_K598014/annotation2153086/range2153086/1
type: Range
persistent identity: range2153086
display id: range2153086
version: 1
direction: direction/forward
top level: BBa_K598014
start: 765
end: 1484
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2153087
display id: annotation2153087
version: 1
top level: BBa_K598014
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K598014/annotation2153087/range2153087/1

https://synbiohub.org/public/igem/BBa_K598014/annotation2153087/range2153087/1
type: Range
persistent identity: range2153087
display id: range2153087
version: 1
direction: direction/forward
top level: BBa_K598014
start: 1493
end: 1621
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2153089
display id: annotation2153089
version: 1
top level: BBa_K598014
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598014/annotation2153089/range2153089/1

https://synbiohub.org/public/igem/BBa_K598014/annotation2153089/range2153089/1
type: Range
persistent identity: range2153089
display id: range2153089
version: 1
direction: direction/forward
top level: BBa_K598014
start: 758
end: 758
orientation: inline

pBAD
type: SequenceAnnotation
persistent identity: annotation2153081
display id: annotation2153081
version: 1
top level: BBa_K598014
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K598014/annotation2153081/range2153081/1

https://synbiohub.org/public/igem/BBa_K598014/annotation2153081/range2153081/1
type: Range
persistent identity: range2153081
display id: range2153081
version: 1
direction: direction/forward
top level: BBa_K598014
start: 1
end: 130
orientation: inline

group I intron
type: SequenceAnnotation
persistent identity: annotation2153083
display id: annotation2153083
version: 1
top level: BBa_K598014
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K598014/annotation2153083/range2153083/1

https://synbiohub.org/public/igem/BBa_K598014/annotation2153083/range2153083/1
type: Range
persistent identity: range2153083
display id: range2153083
version: 1
direction: direction/forward
top level: BBa_K598014
start: 186
end: 764
orientation: inline

pSB1A3
type: ComponentDefinition
persistent identity: pSB1A3
display id: pSB1A3
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: High copy BioBrick assembly plasmid
creator: Reshma Shetty & Tom Knight
created: 2004-05-03T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
m user id: 0
owner id: 52
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: pSB1A3-1 is a high copy number plasmid carrying ampicillin resistance. The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell). pSB1A3 has terminators bracketing its MCS which are designed to prevent transcription from *inside* the MCS from reading out into the vector. The efficiency of these terminators is known to be < 100%. Ideally we would construct a future set of terminators for bracketing a MCS that were 100% efficient in terminating both into and out of the MCS region. A fully annotated Genbank file is attached below.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: rep(pMB1) (rev)
sequence annotation: prefix
sequence annotation: end of terminator
sequence annotation: VR
sequence annotation: ORI
sequence annotation: ampR (rev)
sequence annotation: terminator stem loop
sequence annotation: E. coli his operon terminator
sequence annotation: VF2
sequence annotation: suffix
sequence: https://synbiohub.org/public/igem/pSB1A3_sequence/1

rep(pMB1) (rev)
type: SequenceAnnotation
persistent identity: annotation2119973
display id: annotation2119973
version: 1
top level: pSB1A3
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB1A3/annotation2119973/range2119973/1

https://synbiohub.org/public/igem/pSB1A3/annotation2119973/range2119973/1
type: Range
persistent identity: range2119973
display id: range2119973
version: 1
direction: direction/reverse
top level: pSB1A3
start: 261
end: 875
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation2119979
display id: annotation2119979
version: 1
top level: pSB1A3
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB1A3/annotation2119979/range2119979/1

https://synbiohub.org/public/igem/pSB1A3/annotation2119979/range2119979/1
type: Range
persistent identity: range2119979
display id: range2119979
version: 1
direction: direction/forward
top level: pSB1A3
start: 2136
end: 2157
orientation: inline

end of terminator
type: SequenceAnnotation
persistent identity: annotation2119977
display id: annotation2119977
version: 1
top level: pSB1A3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1A3/annotation2119977/range2119977/1

https://synbiohub.org/public/igem/pSB1A3/annotation2119977/range2119977/1
type: Range
persistent identity: range2119977
display id: range2119977
version: 1
direction: direction/reverse
top level: pSB1A3
start: 2094
end: 2097
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation2119972
display id: annotation2119972
version: 1
top level: pSB1A3
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB1A3/annotation2119972/range2119972/1

https://synbiohub.org/public/igem/pSB1A3/annotation2119972/range2119972/1
type: Range
persistent identity: range2119972
display id: range2119972
version: 1
direction: direction/reverse
top level: pSB1A3
start: 157
end: 176
orientation: inline

ORI
type: SequenceAnnotation
persistent identity: annotation2119974
display id: annotation2119974
version: 1
top level: pSB1A3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1A3/annotation2119974/range2119974/1

https://synbiohub.org/public/igem/pSB1A3/annotation2119974/range2119974/1
type: Range
persistent identity: range2119974
display id: range2119974
version: 1
direction: direction/forward
top level: pSB1A3
start: 276
end: 276
orientation: inline

ampR (rev)
type: SequenceAnnotation
persistent identity: annotation2119975
display id: annotation2119975
version: 1
top level: pSB1A3
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB1A3/annotation2119975/range2119975/1

https://synbiohub.org/public/igem/pSB1A3/annotation2119975/range2119975/1
type: Range
persistent identity: range2119975
display id: range2119975
version: 1
direction: direction/reverse
top level: pSB1A3
start: 1035
end: 1895
orientation: inline

terminator stem loop
type: SequenceAnnotation
persistent identity: annotation2119978
display id: annotation2119978
version: 1
top level: pSB1A3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1A3/annotation2119978/range2119978/1

https://synbiohub.org/public/igem/pSB1A3/annotation2119978/range2119978/1
type: Range
persistent identity: range2119978
display id: range2119978
version: 1
direction: direction/reverse
top level: pSB1A3
start: 2107
end: 2126
orientation: inline

terminator stem loop
type: SequenceAnnotation
persistent identity: annotation2119971
display id: annotation2119971
version: 1
top level: pSB1A3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1A3/annotation2119971/range2119971/1

https://synbiohub.org/public/igem/pSB1A3/annotation2119971/range2119971/1
type: Range
persistent identity: range2119971
display id: range2119971
version: 1
direction: direction/forward
top level: pSB1A3
start: 30
end: 64
orientation: inline

E. coli his operon terminator
type: SequenceAnnotation
persistent identity: annotation2119970
display id: annotation2119970
version: 1
top level: pSB1A3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1A3/annotation2119970/range2119970/1

https://synbiohub.org/public/igem/pSB1A3/annotation2119970/range2119970/1
type: Range
persistent identity: range2119970
display id: range2119970
version: 1
direction: direction/forward
top level: pSB1A3
start: 22
end: 93
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation2119976
display id: annotation2119976
version: 1
top level: pSB1A3
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB1A3/annotation2119976/range2119976/1

https://synbiohub.org/public/igem/pSB1A3/annotation2119976/range2119976/1
type: Range
persistent identity: range2119976
display id: range2119976
version: 1
direction: direction/forward
top level: pSB1A3
start: 2018
end: 2037
orientation: inline

suffix
type: SequenceAnnotation
persistent identity: annotation2119969
display id: annotation2119969
version: 1
top level: pSB1A3
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB1A3/annotation2119969/range2119969/1

https://synbiohub.org/public/igem/pSB1A3/annotation2119969/range2119969/1
type: Range
persistent identity: range2119969
display id: range2119969
version: 1
direction: direction/forward
top level: pSB1A3
start: 1
end: 21
orientation: inline

BBa_K2168000
type: ComponentDefinition
persistent identity: BBa K2168000
display id: BBa_K2168000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBAD/araC - N(6xHis) Ag43
creator: Arunavo Chakraborty
created: 2016-10-12T11:00:00Z
modified: 2016-10-13T02:44:12Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2639_
m user id: 30486
owner id: 30486
owning group id: 9
status: status/Planning
bookmark: true
mutable description: This part encodes E coli protein Ag43, with an additional 6xHis tag for easy purification and quantification. Ag43 expression is known to cause E coli cells to adhere together, and the 6xHis tag was added to allow for convenient study of variation of E coli aggregation with respect to Ag43 expression levels.
mutable notes: Ag43 is an outer membrane E coli protein, so it has a N-terminal 52 amino acid long signal peptide sequence, which is cleaved after Ag43 pro-protein is transported into the periplasmic space. Since strong dependence has been demonstrated on the signal peptide cleavage on the signal peptide-protein junction, the 6xHis tag should be added after Ala53, the residue immediately after the signal peptide sequence.
mutable provenance: This comes from Gibson assembly of fragments from a previous biobrick, BBa_K1352000 by Team:Aberdeen_Scotland, 2014.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: araC
sequence annotation: Double terminator (B0015)
sequence annotation: Ag43(PstI sites removed)
sequence annotation: 6xHis tag
sequence annotation: pBAD
sequence annotation: B0034
sequence: https://synbiohub.org/public/igem/BBa_K2168000_sequence/1

araC
type: SequenceAnnotation
persistent identity: annotation2499395
display id: annotation2499395
version: 1
top level: BBa_K2168000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K2168000/annotation2499395/range2499395/1

https://synbiohub.org/public/igem/BBa_K2168000/annotation2499395/range2499395/1
type: Range
persistent identity: range2499395
display id: range2499395
version: 1
direction: direction/reverse
top level: BBa_K2168000
start: 1
end: 879
orientation: inline

Double terminator (B0015)
type: SequenceAnnotation
persistent identity: annotation2499426
display id: annotation2499426
version: 1
top level: BBa_K2168000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K2168000/annotation2499426/range2499426/1

https://synbiohub.org/public/igem/BBa_K2168000/annotation2499426/range2499426/1
type: Range
persistent identity: range2499426
display id: range2499426
version: 1
direction: direction/forward
top level: BBa_K2168000
start: 4383
end: 4511
orientation: inline

Ag43(PstI sites removed)
type: SequenceAnnotation
persistent identity: annotation2499418
display id: annotation2499418
version: 1
top level: BBa_K2168000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K2168000/annotation2499418/range2499418/1

https://synbiohub.org/public/igem/BBa_K2168000/annotation2499418/range2499418/1
type: Range
persistent identity: range2499418
display id: range2499418
version: 1
direction: direction/forward
top level: BBa_K2168000
start: 1237
end: 4374
orientation: inline

6xHis tag
type: SequenceAnnotation
persistent identity: annotation2499423
display id: annotation2499423
version: 1
top level: BBa_K2168000
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K2168000/annotation2499423/range2499423/1

https://synbiohub.org/public/igem/BBa_K2168000/annotation2499423/range2499423/1
type: Range
persistent identity: range2499423
display id: range2499423
version: 1
direction: direction/forward
top level: BBa_K2168000
start: 1396
end: 1413
orientation: inline

pBAD
type: SequenceAnnotation
persistent identity: annotation2499411
display id: annotation2499411
version: 1
top level: BBa_K2168000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K2168000/annotation2499411/range2499411/1

https://synbiohub.org/public/igem/BBa_K2168000/annotation2499411/range2499411/1
type: Range
persistent identity: range2499411
display id: range2499411
version: 1
direction: direction/forward
top level: BBa_K2168000
start: 906
end: 1190
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2499416
display id: annotation2499416
version: 1
top level: BBa_K2168000
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K2168000/annotation2499416/range2499416/1

https://synbiohub.org/public/igem/BBa_K2168000/annotation2499416/range2499416/1
type: Range
persistent identity: range2499416
display id: range2499416
version: 1
direction: direction/forward
top level: BBa_K2168000
start: 1219
end: 1230
orientation: inline