//plasmid/measurement
type: Collection
persistent identity: plasmid measurement
display id: plasmid_measurement
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: Screening
member: BBa_J23101
member: GFP report
member: BBa_K292007
member: BBa_K731700
member: BBa_I714945
member: BBa_K256002
member: BBa_K545001
member: pSB3K3

BBa_J23101
type: ComponentDefinition
persistent identity: BBa J23101
display id: BBa_J23101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: later
mutable notes: N/A
mutable provenance: later
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23101_sequence/1

Screening
type: ComponentDefinition
persistent identity: pSB1A10
display id: pSB1A10
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Screening Plasmid v1.0
creator: Josh Michener, Jason Kelly
created: 2006-05-25T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_
m user id: 0
owner id: 2
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: suffix
sequence annotation: I0500 (pBAD/araC)
sequence annotation: spacer artifact from 13450
sequence annotation: B0034
sequence annotation: Hairpin
sequence annotation: E1010 (mRFP1)
sequence annotation: RNAse E site
sequence annotation: Scar from BB suffix removal by I13450
sequence annotation: Scar from BB prefix removal by I13450
sequence annotation: E0040 (EGFP)
sequence annotation: barcode
sequence annotation: psb1a3
sequence annotation: prefix
sequence annotation: B0015 (Term)
sequence: https://synbiohub.org/public/igem/pSB1A10_sequence/1

suffix
type: SequenceAnnotation
persistent identity: annotation1871283
display id: annotation1871283
version: 1
top level: Screening
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB1A10/annotation1871283/range1871283/1

https://synbiohub.org/public/igem/pSB1A10/annotation1871283/range1871283/1
type: Range
persistent identity: range1871283
display id: range1871283
version: 1
direction: direction/forward
top level: Screening
start: 1
end: 21
orientation: inline

I0500 (pBAD/araC)
type: SequenceAnnotation
persistent identity: annotation1871315
display id: annotation1871315
version: 1
top level: Screening
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/pSB1A10/annotation1871315/range1871315/1

https://synbiohub.org/public/igem/pSB1A10/annotation1871315/range1871315/1
type: Range
persistent identity: range1871315
display id: range1871315
version: 1
direction: direction/forward
top level: Screening
start: 3239
end: 4448
orientation: inline

spacer artifact from 13450
type: SequenceAnnotation
persistent identity: annotation1871305
display id: annotation1871305
version: 1
top level: Screening
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1A10/annotation1871305/range1871305/1

https://synbiohub.org/public/igem/pSB1A10/annotation1871305/range1871305/1
type: Range
persistent identity: range1871305
display id: range1871305
version: 1
direction: direction/forward
top level: Screening
start: 22
end: 37
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation1871308
display id: annotation1871308
version: 1
top level: Screening
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/pSB1A10/annotation1871308/range1871308/1

https://synbiohub.org/public/igem/pSB1A10/annotation1871308/range1871308/1
type: Range
persistent identity: range1871308
display id: range1871308
version: 1
direction: direction/forward
top level: Screening
start: 102
end: 113
orientation: inline

Hairpin
type: SequenceAnnotation
persistent identity: annotation1871307
display id: annotation1871307
version: 1
top level: Screening
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB1A10/annotation1871307/range1871307/1

https://synbiohub.org/public/igem/pSB1A10/annotation1871307/range1871307/1
type: Range
persistent identity: range1871307
display id: range1871307
version: 1
direction: direction/forward
top level: Screening
start: 59
end: 93
orientation: inline

E1010 (mRFP1)
type: SequenceAnnotation
persistent identity: annotation1871309
display id: annotation1871309
version: 1
top level: Screening
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB1A10/annotation1871309/range1871309/1

https://synbiohub.org/public/igem/pSB1A10/annotation1871309/range1871309/1
type: Range
persistent identity: range1871309
display id: range1871309
version: 1
direction: direction/forward
top level: Screening
start: 120
end: 800
orientation: inline

RNAse E site
type: SequenceAnnotation
persistent identity: annotation1871306
display id: annotation1871306
version: 1
top level: Screening
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1A10/annotation1871306/range1871306/1

https://synbiohub.org/public/igem/pSB1A10/annotation1871306/range1871306/1
type: Range
persistent identity: range1871306
display id: range1871306
version: 1
direction: direction/forward
top level: Screening
start: 38
end: 58
orientation: inline

Scar from BB suffix removal by I13450
type: SequenceAnnotation
persistent identity: annotation1871312
display id: annotation1871312
version: 1
top level: Screening
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1A10/annotation1871312/range1871312/1

https://synbiohub.org/public/igem/pSB1A10/annotation1871312/range1871312/1
type: Range
persistent identity: range1871312
display id: range1871312
version: 1
direction: direction/forward
top level: Screening
start: 963
end: 1044
orientation: inline

RNAse E site
type: SequenceAnnotation
persistent identity: annotation1871318
display id: annotation1871318
version: 1
top level: Screening
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1A10/annotation1871318/range1871318/1

https://synbiohub.org/public/igem/pSB1A10/annotation1871318/range1871318/1
type: Range
persistent identity: range1871318
display id: range1871318
version: 1
direction: direction/forward
top level: Screening
start: 5203
end: 5223
orientation: inline

Scar from BB prefix removal by I13450
type: SequenceAnnotation
persistent identity: annotation1871314
display id: annotation1871314
version: 1
top level: Screening
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1A10/annotation1871314/range1871314/1

https://synbiohub.org/public/igem/pSB1A10/annotation1871314/range1871314/1
type: Range
persistent identity: range1871314
display id: range1871314
version: 1
direction: direction/forward
top level: Screening
start: 3158
end: 3238
orientation: inline

E0040 (EGFP)
type: SequenceAnnotation
persistent identity: annotation1871317
display id: annotation1871317
version: 1
top level: Screening
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB1A10/annotation1871317/range1871317/1

https://synbiohub.org/public/igem/pSB1A10/annotation1871317/range1871317/1
type: Range
persistent identity: range1871317
display id: range1871317
version: 1
direction: direction/forward
top level: Screening
start: 4475
end: 5194
orientation: inline

barcode
type: SequenceAnnotation
persistent identity: annotation1871310
display id: annotation1871310
version: 1
top level: Screening
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/pSB1A10/annotation1871310/range1871310/1

https://synbiohub.org/public/igem/pSB1A10/annotation1871310/range1871310/1
type: Range
persistent identity: range1871310
display id: range1871310
version: 1
direction: direction/forward
top level: Screening
start: 801
end: 825
orientation: inline

psb1a3
type: SequenceAnnotation
persistent identity: annotation1871313
display id: annotation1871313
version: 1
top level: Screening
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1A10/annotation1871313/range1871313/1

https://synbiohub.org/public/igem/pSB1A10/annotation1871313/range1871313/1
type: Range
persistent identity: range1871313
display id: range1871313
version: 1
direction: direction/forward
top level: Screening
start: 1045
end: 3157
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation1871320
display id: annotation1871320
version: 1
top level: Screening
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB1A10/annotation1871320/range1871320/1

https://synbiohub.org/public/igem/pSB1A10/annotation1871320/range1871320/1
type: Range
persistent identity: range1871320
display id: range1871320
version: 1
direction: direction/forward
top level: Screening
start: 5242
end: 5263
orientation: inline

spacer artifact from 13450
type: SequenceAnnotation
persistent identity: annotation1871319
display id: annotation1871319
version: 1
top level: Screening
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1A10/annotation1871319/range1871319/1

https://synbiohub.org/public/igem/pSB1A10/annotation1871319/range1871319/1
type: Range
persistent identity: range1871319
display id: range1871319
version: 1
direction: direction/forward
top level: Screening
start: 5224
end: 5241
orientation: inline

B0015 (Term)
type: SequenceAnnotation
persistent identity: annotation1871311
display id: annotation1871311
version: 1
top level: Screening
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB1A10/annotation1871311/range1871311/1

https://synbiohub.org/public/igem/pSB1A10/annotation1871311/range1871311/1
type: Range
persistent identity: range1871311
display id: range1871311
version: 1
direction: direction/forward
top level: Screening
start: 834
end: 962
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation1871316
display id: annotation1871316
version: 1
top level: Screening
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/pSB1A10/annotation1871316/range1871316/1

https://synbiohub.org/public/igem/pSB1A10/annotation1871316/range1871316/1
type: Range
persistent identity: range1871316
display id: range1871316
version: 1
direction: direction/forward
top level: Screening
start: 4457
end: 4468
orientation: inline

GFP report
type: ComponentDefinition
persistent identity: BBa E0240
display id: BBa_E0240
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP generator
creator: Jennifer Braff
created: 2004-10-17T11:00:00Z
modified: 2015-08-31T04:07:26Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: B0032.E0040.B0015
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0032
component: BBa_B0012
component: BBa_E0040
component: BBa_B0010
sequence annotation: BBa_E0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0032
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_E0240_sequence/1

BBa_B0032
type: Component
persistent identity: component1249213
display id: component1249213
version: 1
top level: GFP report
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_B0012
type: Component
persistent identity: component1249231
display id: component1249231
version: 1
top level: GFP report
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E0040
type: Component
persistent identity: component1249216
display id: component1249216
version: 1
top level: GFP report
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0010
type: Component
persistent identity: component1249221
display id: component1249221
version: 1
top level: GFP report
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1249216
display id: annotation1249216
version: 1
top level: GFP report
location: https://synbiohub.org/public/igem/BBa_E0240/annotation1249216/range1249216/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_E0240/annotation1249216/range1249216/1
type: Range
persistent identity: range1249216
display id: range1249216
version: 1
direction: direction/forward
top level: GFP report
start: 20
end: 739
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1249221
display id: annotation1249221
version: 1
top level: GFP report
location: https://synbiohub.org/public/igem/BBa_E0240/annotation1249221/range1249221/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_E0240/annotation1249221/range1249221/1
type: Range
persistent identity: range1249221
display id: range1249221
version: 1
direction: direction/forward
top level: GFP report
start: 748
end: 827
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1249213
display id: annotation1249213
version: 1
top level: GFP report
location: https://synbiohub.org/public/igem/BBa_E0240/annotation1249213/range1249213/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_E0240/annotation1249213/range1249213/1
type: Range
persistent identity: range1249213
display id: range1249213
version: 1
direction: direction/forward
top level: GFP report
start: 1
end: 13
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1249231
display id: annotation1249231
version: 1
top level: GFP report
location: https://synbiohub.org/public/igem/BBa_E0240/annotation1249231/range1249231/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_E0240/annotation1249231/range1249231/1
type: Range
persistent identity: range1249231
display id: range1249231
version: 1
direction: direction/forward
top level: GFP report
start: 836
end: 876
orientation: inline

BBa_K292007
type: ComponentDefinition
persistent identity: BBa K292007
display id: BBa_K292007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CMV + GFP + Double terminator
creator: David Charoy
created: 2009-10-18T11:00:00Z
modified: 2015-05-08T01:11:49Z
discontinued: false
dominant: false
group u list: _394_
m user id: 0
owner id: 5212
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part contains a green fluorescence protein (GFP) under control of a CMV promoter and its double terminator (BBa_I712669 + BBa_B0014). This part is reliable to use when we want to know if a gene is inserted into a eukaryotic cell. For example we can use it when we design a recombinant virus. In fact we inserted this bio-brick into the synthetic virus; finally we are able to see if the virus can infect the targeted cells. This bio-brick already contains a terminator, so it is ready to use.
mutable notes: N/A
mutable provenance: Part:BBa_I712669, Designed by Rok Gaber Group: iGEM07_Ljubljana (2007-10-24) Part:BBa_B0014, Designed by Reshma Shetty Group: Registry (2003-07-16)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J52642
component: BBa_B0011
component: BBa_B0012
component: BBa_I712004
sequence annotation: BBa_B0012
sequence annotation: BBa_J52642
sequence annotation: BBa_I712004
sequence annotation: BBa_B0011
sequence: https://synbiohub.org/public/igem/BBa_K292007_sequence/1

BBa_J52642
type: Component
persistent identity: component2054628
display id: component2054628
version: 1
top level: BBa_K292007
access: public
definition: https://synbiohub.org/public/igem/BBa_J52642/1

BBa_B0011
type: Component
persistent identity: component2054633
display id: component2054633
version: 1
top level: BBa_K292007
access: public
definition: https://synbiohub.org/public/igem/BBa_B0011/1

BBa_B0012
type: Component
persistent identity: component2054629
display id: component2054629
version: 1
top level: BBa_K292007
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_I712004
type: Component
persistent identity: component2054624
display id: component2054624
version: 1
top level: BBa_K292007
access: public
definition: https://synbiohub.org/public/igem/BBa_I712004/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2054629
display id: annotation2054629
version: 1
top level: BBa_K292007
location: https://synbiohub.org/public/igem/BBa_K292007/annotation2054629/range2054629/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K292007/annotation2054629/range2054629/1
type: Range
persistent identity: range2054629
display id: range2054629
version: 1
direction: direction/forward
top level: BBa_K292007
start: 1389
end: 1429
orientation: inline

BBa_J52642
type: SequenceAnnotation
persistent identity: annotation2054628
display id: annotation2054628
version: 1
top level: BBa_K292007
location: https://synbiohub.org/public/igem/BBa_K292007/annotation2054628/range2054628/1
component: BBa_J52642

https://synbiohub.org/public/igem/BBa_K292007/annotation2054628/range2054628/1
type: Range
persistent identity: range2054628
display id: range2054628
version: 1
direction: direction/reverse
top level: BBa_K292007
start: 661
end: 1380
orientation: inline

BBa_I712004
type: SequenceAnnotation
persistent identity: annotation2054624
display id: annotation2054624
version: 1
top level: BBa_K292007
location: https://synbiohub.org/public/igem/BBa_K292007/annotation2054624/range2054624/1
component: BBa_I712004

https://synbiohub.org/public/igem/BBa_K292007/annotation2054624/range2054624/1
type: Range
persistent identity: range2054624
display id: range2054624
version: 1
direction: direction/reverse
top level: BBa_K292007
start: 1
end: 654
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation2054633
display id: annotation2054633
version: 1
top level: BBa_K292007
location: https://synbiohub.org/public/igem/BBa_K292007/annotation2054633/range2054633/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_K292007/annotation2054633/range2054633/1
type: Range
persistent identity: range2054633
display id: range2054633
version: 1
direction: direction/forward
top level: BBa_K292007
start: 1438
end: 1483
orientation: inline

BBa_I714945
type: ComponentDefinition
persistent identity: BBa I714945
display id: BBa_I714945
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLX012
creator: Xili Liu
created: 2007-10-24T11:00:00Z
modified: 2015-08-31T04:07:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _142_
m user id: 0
owner id: 2250
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: a plasmid with EGFP reporter to report double repressed promoter activity
mutable notes: EGFP with strong SD signal, a MCS before EGFP to insert double repressed promoter, high efficient double terminator T1T2 locates on both upstream of MCS and downstream of EGFP.
mutable provenance: pCRT-1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
sequence annotation: MCS
sequence annotation: ColE ori
sequence annotation: Amp
sequence annotation: T1T2
sequence annotation: stop
sequence annotation: EGFP
sequence annotation: SDY
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_I714945_sequence/1

MCS
type: SequenceAnnotation
persistent identity: annotation1955156
display id: annotation1955156
version: 1
top level: BBa_I714945
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_I714945/annotation1955156/range1955156/1

https://synbiohub.org/public/igem/BBa_I714945/annotation1955156/range1955156/1
type: Range
persistent identity: range1955156
display id: range1955156
version: 1
direction: direction/forward
top level: BBa_I714945
start: 229
end: 246
orientation: inline

ColE ori
type: SequenceAnnotation
persistent identity: annotation1955157
display id: annotation1955157
version: 1
top level: BBa_I714945
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_I714945/annotation1955157/range1955157/1

https://synbiohub.org/public/igem/BBa_I714945/annotation1955157/range1955157/1
type: Range
persistent identity: range1955157
display id: range1955157
version: 1
direction: direction/reverse
top level: BBa_I714945
start: 1494
end: 2082
orientation: inline

Amp
type: SequenceAnnotation
persistent identity: annotation1955155
display id: annotation1955155
version: 1
top level: BBa_I714945
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_I714945/annotation1955155/range1955155/1

https://synbiohub.org/public/igem/BBa_I714945/annotation1955155/range1955155/1
type: Range
persistent identity: range1955155
display id: range1955155
version: 1
direction: direction/reverse
top level: BBa_I714945
start: 2264
end: 3195
orientation: inline

T1T2
type: SequenceAnnotation
persistent identity: annotation1955132
display id: annotation1955132
version: 1
top level: BBa_I714945
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I714945/annotation1955132/range1955132/1

https://synbiohub.org/public/igem/BBa_I714945/annotation1955132/range1955132/1
type: Range
persistent identity: range1955132
display id: range1955132
version: 1
direction: direction/forward
top level: BBa_I714945
start: 1
end: 203
orientation: inline

T1T2
type: SequenceAnnotation
persistent identity: annotation1955154
display id: annotation1955154
version: 1
top level: BBa_I714945
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I714945/annotation1955154/range1955154/1

https://synbiohub.org/public/igem/BBa_I714945/annotation1955154/range1955154/1
type: Range
persistent identity: range1955154
display id: range1955154
version: 1
direction: direction/forward
top level: BBa_I714945
start: 1028
end: 1230
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1955153
display id: annotation1955153
version: 1
top level: BBa_I714945
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I714945/annotation1955153/range1955153/1

https://synbiohub.org/public/igem/BBa_I714945/annotation1955153/range1955153/1
type: Range
persistent identity: range1955153
display id: range1955153
version: 1
direction: direction/forward
top level: BBa_I714945
start: 967
end: 967
orientation: inline

EGFP
type: SequenceAnnotation
persistent identity: annotation1955152
display id: annotation1955152
version: 1
top level: BBa_I714945
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_I714945/annotation1955152/range1955152/1

https://synbiohub.org/public/igem/BBa_I714945/annotation1955152/range1955152/1
type: Range
persistent identity: range1955152
display id: range1955152
version: 1
direction: direction/forward
top level: BBa_I714945
start: 263
end: 967
orientation: inline

SDY
type: SequenceAnnotation
persistent identity: annotation1955150
display id: annotation1955150
version: 1
top level: BBa_I714945
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_I714945/annotation1955150/range1955150/1

https://synbiohub.org/public/igem/BBa_I714945/annotation1955150/range1955150/1
type: Range
persistent identity: range1955150
display id: range1955150
version: 1
direction: direction/forward
top level: BBa_I714945
start: 248
end: 262
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1955151
display id: annotation1955151
version: 1
top level: BBa_I714945
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I714945/annotation1955151/range1955151/1

https://synbiohub.org/public/igem/BBa_I714945/annotation1955151/range1955151/1
type: Range
persistent identity: range1955151
display id: range1955151
version: 1
direction: direction/forward
top level: BBa_I714945
start: 263
end: 263
orientation: inline

BBa_K731700
type: ComponentDefinition
persistent identity: BBa K731700
display id: BBa_K731700
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Platform for terminators analysis under the control of T7 promoter
creator: Giacomo Giacomelli, Anna Depetris
created: 2012-08-13T11:00:00Z
modified: 2015-05-08T01:13:06Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _977_
m user id: 0
owner id: 12063
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: We used this part to test terminators: the two subsequent proteins allow to quantify the termination efficiency as the ratio between Venus's and Cherry's fluorescence. The vector is pET21b, that contains lacI, t7 promoter and ampicillin resistance gene. The two fluorescent proteins have partially overlapping emission spectra, thus the fluorimetric measures should be taken with these parameters, to avoid interference between the two: Venus excitation: 485nm, Venus emission: 528nm Cherry excitation: 528nm, Cherry emission: 615nm We inserted the prefix-suffix linker between the two proteins, to allow terminator characterization.
mutable notes: Using and modifying this construct is important to remember that the two proteins have strong N-terminal homology, especially designing primers. For any future improvement, maybe it would be better two use two different fluorescence proteins, because these two has partially overlapping emission spectra and different folding kinetics. Using and modifying this construct is important to remember that the two proteins have strong N-terminal homology, especially designing primers. For any future improvement, maybe it would be better two use two different fluorescence proteins, because these two has partially overlapping emission spectra and different folding kinetics.
mutable provenance: It's made from a Roberta Lentini's construct (from Mansy lab) that consist of pET21b with the two proteins and a 20bp linker between them. That construct was mutated two times to eliminate illegal restriction sites; prefix-suffix linker was added by insertion PCR.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: ampicillin resistance gene
sequence annotation: T7 PROMOTER
sequence annotation: A206K VENUS
sequence annotation: mCHERRY
sequence annotation: T7 TERMINATOR
sequence annotation: lacO
sequence annotation: lacI
sequence: https://synbiohub.org/public/igem/BBa_K731700_sequence/1

ampicillin resistance gene
type: SequenceAnnotation
persistent identity: annotation2179558
display id: annotation2179558
version: 1
top level: BBa_K731700
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K731700/annotation2179558/range2179558/1

https://synbiohub.org/public/igem/BBa_K731700/annotation2179558/range2179558/1
type: Range
persistent identity: range2179558
display id: range2179558
version: 1
direction: direction/forward
top level: BBa_K731700
start: 1711
end: 2370
orientation: inline

T7 PROMOTER
type: SequenceAnnotation
persistent identity: annotation2179560
display id: annotation2179560
version: 1
top level: BBa_K731700
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K731700/annotation2179560/range2179560/1

https://synbiohub.org/public/igem/BBa_K731700/annotation2179560/range2179560/1
type: Range
persistent identity: range2179560
display id: range2179560
version: 1
direction: direction/forward
top level: BBa_K731700
start: 6031
end: 6050
orientation: inline

A206K VENUS
type: SequenceAnnotation
persistent identity: annotation2179556
display id: annotation2179556
version: 1
top level: BBa_K731700
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K731700/annotation2179556/range2179556/1

https://synbiohub.org/public/igem/BBa_K731700/annotation2179556/range2179556/1
type: Range
persistent identity: range2179556
display id: range2179556
version: 1
direction: direction/forward
top level: BBa_K731700
start: 36
end: 752
orientation: inline

mCHERRY
type: SequenceAnnotation
persistent identity: annotation2179562
display id: annotation2179562
version: 1
top level: BBa_K731700
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K731700/annotation2179562/range2179562/1

https://synbiohub.org/public/igem/BBa_K731700/annotation2179562/range2179562/1
type: Range
persistent identity: range2179562
display id: range2179562
version: 1
direction: direction/forward
top level: BBa_K731700
start: 6119
end: 6829
orientation: inline

T7 TERMINATOR
type: SequenceAnnotation
persistent identity: annotation2179557
display id: annotation2179557
version: 1
top level: BBa_K731700
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K731700/annotation2179557/range2179557/1

https://synbiohub.org/public/igem/BBa_K731700/annotation2179557/range2179557/1
type: Range
persistent identity: range2179557
display id: range2179557
version: 1
direction: direction/forward
top level: BBa_K731700
start: 843
end: 890
orientation: inline

lacO
type: SequenceAnnotation
persistent identity: annotation2179561
display id: annotation2179561
version: 1
top level: BBa_K731700
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K731700/annotation2179561/range2179561/1

https://synbiohub.org/public/igem/BBa_K731700/annotation2179561/range2179561/1
type: Range
persistent identity: range2179561
display id: range2179561
version: 1
direction: direction/forward
top level: BBa_K731700
start: 6050
end: 6072
orientation: inline

lacI
type: SequenceAnnotation
persistent identity: annotation2179559
display id: annotation2179559
version: 1
top level: BBa_K731700
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K731700/annotation2179559/range2179559/1

https://synbiohub.org/public/igem/BBa_K731700/annotation2179559/range2179559/1
type: Range
persistent identity: range2179559
display id: range2179559
version: 1
direction: direction/reverse
top level: BBa_K731700
start: 4562
end: 5653
orientation: inline

BBa_K256002
type: ComponentDefinition
persistent identity: BBa K256002
display id: BBa_K256002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23101:GFP
creator: iGEM09_NTU-Singapore
created: 2009-10-15T11:00:00Z
modified: 2015-05-08T01:11:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _359_
m user id: 0
owner id: 4864
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: GFP Reporter
mutable notes: --
mutable provenance: --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_J23101
component: BBa_B0034
component: BBa_E0040
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_E0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_J23101
sequence: https://synbiohub.org/public/igem/BBa_K256002_sequence/1

BBa_J23101
type: Component
persistent identity: component2056715
display id: component2056715
version: 1
top level: BBa_K256002
access: public
definition: BBa_J23101

BBa_B0034
type: Component
persistent identity: component2056717
display id: component2056717
version: 1
top level: BBa_K256002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0040
type: Component
persistent identity: component2056719
display id: component2056719
version: 1
top level: BBa_K256002
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0012
type: Component
persistent identity: component2056722
display id: component2056722
version: 1
top level: BBa_K256002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component2056720
display id: component2056720
version: 1
top level: BBa_K256002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2056722
display id: annotation2056722
version: 1
top level: BBa_K256002
location: https://synbiohub.org/public/igem/BBa_K256002/annotation2056722/range2056722/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K256002/annotation2056722/range2056722/1
type: Range
persistent identity: range2056722
display id: range2056722
version: 1
direction: direction/forward
top level: BBa_K256002
start: 878
end: 918
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2056719
display id: annotation2056719
version: 1
top level: BBa_K256002
location: https://synbiohub.org/public/igem/BBa_K256002/annotation2056719/range2056719/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K256002/annotation2056719/range2056719/1
type: Range
persistent identity: range2056719
display id: range2056719
version: 1
direction: direction/reverse
top level: BBa_K256002
start: 62
end: 781
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2056720
display id: annotation2056720
version: 1
top level: BBa_K256002
location: https://synbiohub.org/public/igem/BBa_K256002/annotation2056720/range2056720/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K256002/annotation2056720/range2056720/1
type: Range
persistent identity: range2056720
display id: range2056720
version: 1
direction: direction/forward
top level: BBa_K256002
start: 790
end: 869
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2056717
display id: annotation2056717
version: 1
top level: BBa_K256002
location: https://synbiohub.org/public/igem/BBa_K256002/annotation2056717/range2056717/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K256002/annotation2056717/range2056717/1
type: Range
persistent identity: range2056717
display id: range2056717
version: 1
direction: direction/reverse
top level: BBa_K256002
start: 44
end: 55
orientation: inline

BBa_J23101
type: SequenceAnnotation
persistent identity: annotation2056715
display id: annotation2056715
version: 1
top level: BBa_K256002
location: https://synbiohub.org/public/igem/BBa_K256002/annotation2056715/range2056715/1
component: BBa_J23101

https://synbiohub.org/public/igem/BBa_K256002/annotation2056715/range2056715/1
type: Range
persistent identity: range2056715
display id: range2056715
version: 1
direction: direction/reverse
top level: BBa_K256002
start: 1
end: 35
orientation: inline

pSB3K3
type: ComponentDefinition
persistent identity: pSB3K3
display id: pSB3K3
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low to medium copy BioBrick standard vector
creator: Reshma Shetty
created: 2004-06-28T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
m user id: 0
owner id: 60
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: pSB3K3-1 is a medium copy plasmid with kanamycin resistance. It has a p15A pMR101-derived replication origin, copy number 20-30. (Lutz and Bujard, 1997) pSB3K3-1 has a terminator upstream of its MCS, which is oriented to prevent transcription from *inside* the MCS from reading out into the vectorl. A second terminator (E.coli His operon-derived) is downstream of the MCS, again insulating the vector from transcription reading out of the MCS. Ideally, future versions of standard biobrick vectors would have terminators bracketing the MCS that were 100% efficient in terminating transcription both into and out of the MCS region.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Prefix
sequence annotation: p15A ORI (rev)
sequence annotation: E. coli his operon terminator
sequence annotation: terminator stem loop
sequence annotation: end of terminator
sequence annotation: VF2
sequence annotation: VR
sequence annotation: deletion of two bases
sequence annotation: Suffix
sequence annotation: kanR (rev)
sequence: https://synbiohub.org/public/igem/pSB3K3_sequence/1

Prefix
type: SequenceAnnotation
persistent identity: annotation1023043
display id: annotation1023043
version: 1
top level: pSB3K3
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB3K3/annotation1023043/range1023043/1

https://synbiohub.org/public/igem/pSB3K3/annotation1023043/range1023043/1
type: Range
persistent identity: range1023043
display id: range1023043
version: 1
direction: direction/forward
top level: pSB3K3
start: 2729
end: 2750
orientation: inline

p15A ORI (rev)
type: SequenceAnnotation
persistent identity: annotation1023046
display id: annotation1023046
version: 1
top level: pSB3K3
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB3K3/annotation1023046/range1023046/1

https://synbiohub.org/public/igem/pSB3K3/annotation1023046/range1023046/1
type: Range
persistent identity: range1023046
display id: range1023046
version: 1
direction: direction/reverse
top level: pSB3K3
start: 1386
end: 2210
orientation: inline

E. coli his operon terminator
type: SequenceAnnotation
persistent identity: annotation1824021
display id: annotation1824021
version: 1
top level: pSB3K3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB3K3/annotation1824021/range1824021/1

https://synbiohub.org/public/igem/pSB3K3/annotation1824021/range1824021/1
type: Range
persistent identity: range1824021
display id: range1824021
version: 1
direction: direction/forward
top level: pSB3K3
start: 22
end: 93
orientation: inline

terminator stem loop
type: SequenceAnnotation
persistent identity: annotation1824022
display id: annotation1824022
version: 1
top level: pSB3K3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB3K3/annotation1824022/range1824022/1

https://synbiohub.org/public/igem/pSB3K3/annotation1824022/range1824022/1
type: Range
persistent identity: range1824022
display id: range1824022
version: 1
direction: direction/forward
top level: pSB3K3
start: 30
end: 64
orientation: inline

end of terminator
type: SequenceAnnotation
persistent identity: annotation1824024
display id: annotation1824024
version: 1
top level: pSB3K3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB3K3/annotation1824024/range1824024/1

https://synbiohub.org/public/igem/pSB3K3/annotation1824024/range1824024/1
type: Range
persistent identity: range1824024
display id: range1824024
version: 1
direction: direction/reverse
top level: pSB3K3
start: 2687
end: 2690
orientation: inline

terminator stem loop
type: SequenceAnnotation
persistent identity: annotation1824023
display id: annotation1824023
version: 1
top level: pSB3K3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB3K3/annotation1824023/range1824023/1

https://synbiohub.org/public/igem/pSB3K3/annotation1824023/range1824023/1
type: Range
persistent identity: range1824023
display id: range1824023
version: 1
direction: direction/reverse
top level: pSB3K3
start: 2700
end: 2719
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation1782459
display id: annotation1782459
version: 1
top level: pSB3K3
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB3K3/annotation1782459/range1782459/1

https://synbiohub.org/public/igem/pSB3K3/annotation1782459/range1782459/1
type: Range
persistent identity: range1782459
display id: range1782459
version: 1
direction: direction/forward
top level: pSB3K3
start: 2611
end: 2630
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation1782476
display id: annotation1782476
version: 1
top level: pSB3K3
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB3K3/annotation1782476/range1782476/1

https://synbiohub.org/public/igem/pSB3K3/annotation1782476/range1782476/1
type: Range
persistent identity: range1782476
display id: range1782476
version: 1
direction: direction/reverse
top level: pSB3K3
start: 157
end: 176
orientation: inline

deletion of two bases
type: SequenceAnnotation
persistent identity: annotation1823788
display id: annotation1823788
version: 1
top level: pSB3K3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB3K3/annotation1823788/range1823788/1

https://synbiohub.org/public/igem/pSB3K3/annotation1823788/range1823788/1
type: Range
persistent identity: range1823788
display id: range1823788
version: 1
direction: direction/forward
top level: pSB3K3
start: 34
end: 35
orientation: inline

Suffix
type: SequenceAnnotation
persistent identity: annotation1023044
display id: annotation1023044
version: 1
top level: pSB3K3
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB3K3/annotation1023044/range1023044/1

https://synbiohub.org/public/igem/pSB3K3/annotation1023044/range1023044/1
type: Range
persistent identity: range1023044
display id: range1023044
version: 1
direction: direction/forward
top level: pSB3K3
start: 1
end: 21
orientation: inline

kanR (rev)
type: SequenceAnnotation
persistent identity: annotation1023045
display id: annotation1023045
version: 1
top level: pSB3K3
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB3K3/annotation1023045/range1023045/1

https://synbiohub.org/public/igem/pSB3K3/annotation1023045/range1023045/1
type: Range
persistent identity: range1023045
display id: range1023045
version: 1
direction: direction/reverse
top level: pSB3K3
start: 239
end: 1054
orientation: inline

BBa_K545001
type: ComponentDefinition
persistent identity: BBa K545001
display id: BBa_K545001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pConst+GFP
creator: Grenoble 2011
created: 2011-09-19T11:00:00Z
modified: 2015-05-08T01:12:39Z
discontinued: false
dominant: false
experience: experience/None
group u list: _712_
m user id: 0
owner id: 8648
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Green fluorescent protein (E0240) is constitutively produced thanks to a constitutive promoter (J23119).
mutable notes: This part was build following the standard construction protocol.
mutable provenance: J23119 is an E.coli constitutive promoter. The GFP sequence included in E0240 is a green fluorescent protein derived from jellyfish Aequeora victoria wild type GFP.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_J23119
component: BBa_E0240
sequence annotation: BBa_J23119
sequence annotation: BBa_E0240
sequence: https://synbiohub.org/public/igem/BBa_K545001_sequence/1

BBa_J23119
type: Component
persistent identity: component2135394
display id: component2135394
version: 1
top level: BBa_K545001
access: public
definition: https://synbiohub.org/public/igem/BBa_J23119/1

BBa_E0240
type: Component
persistent identity: component2135405
display id: component2135405
version: 1
top level: BBa_K545001
access: public
definition: GFP report

BBa_J23119
type: SequenceAnnotation
persistent identity: annotation2135394
display id: annotation2135394
version: 1
top level: BBa_K545001
location: https://synbiohub.org/public/igem/BBa_K545001/annotation2135394/range2135394/1
component: BBa_J23119

https://synbiohub.org/public/igem/BBa_K545001/annotation2135394/range2135394/1
type: Range
persistent identity: range2135394
display id: range2135394
version: 1
direction: direction/reverse
top level: BBa_K545001
start: 1
end: 35
orientation: inline

BBa_E0240
type: SequenceAnnotation
persistent identity: annotation2135405
display id: annotation2135405
version: 1
top level: BBa_K545001
location: https://synbiohub.org/public/igem/BBa_K545001/annotation2135405/range2135405/1
component: BBa_E0240

https://synbiohub.org/public/igem/BBa_K545001/annotation2135405/range2135405/1
type: Range
persistent identity: range2135405
display id: range2135405
version: 1
direction: direction/reverse
top level: BBa_K545001
start: 44
end: 919
orientation: inline