//plasmid/expression/t7
type: Collection
persistent identity: plasmid expression t7
display id: plasmid_expression_t7
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_K1428000
member: BBa_K533003
member: BBa_K921001
member: T7start
member: BBa_K1680026
member: T7RSR
member: BBa_K559010
member: BBa_K533002
member: BBa_K921002
member: BBa_K921000
member: BBa_K1614000
member: BBa_K533001
member: T7p
member: T7stop
member: BBa_K559001

BBa_K1680026
type: ComponentDefinition
persistent identity: BBa K1680026
display id: BBa_K1680026
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pETUE
creator: Katharina Sporbeck
created: 2015-09-17T11:00:00Z
modified: 2015-10-28T03:58:14Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2098_
m user id: 15693
owner id: 26460
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Expression Plasmid coding for His-tag, also containing a thrombine cleavage site between coding region and Histag, so that His-tag can be cleaved off. Vecotr opening with PstI/XmaI, clone part into it with PstI/NgoMIV. Ampicillin resistance.
mutable notes: very strong RBS 6xHis-Tag Thromin-cleavage-site NgoMIV-compatible restriction site for in-frame insertion
mutable provenance: pET-Vector
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: 6x His-tag
sequence annotation: T7 promoter
sequence annotation: ori
sequence annotation: thrombin cleavage site
sequence annotation: Lac operator
sequence annotation: T7 terminator
sequence annotation: tet promoter
sequence annotation: lac operator
sequence annotation: f1 ori
sequence annotation: RBS.1 strong
sequence annotation: AmpR
sequence annotation: rrnB T1 terminator
sequence: https://synbiohub.org/public/igem/BBa_K1680026_sequence/1

6x His-tag
type: SequenceAnnotation
persistent identity: annotation2472216
display id: annotation2472216
version: 1
top level: BBa_K1680026
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1680026/annotation2472216/range2472216/1

https://synbiohub.org/public/igem/BBa_K1680026/annotation2472216/range2472216/1
type: Range
persistent identity: range2472216
display id: range2472216
version: 1
direction: direction/forward
top level: BBa_K1680026
start: 77
end: 94
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2472193
display id: annotation2472193
version: 1
top level: BBa_K1680026
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680026/annotation2472193/range2472193/1

https://synbiohub.org/public/igem/BBa_K1680026/annotation2472193/range2472193/1
type: Range
persistent identity: range2472193
display id: range2472193
version: 1
direction: direction/forward
top level: BBa_K1680026
start: 1
end: 19
orientation: inline

ori
type: SequenceAnnotation
persistent identity: annotation2472305
display id: annotation2472305
version: 1
top level: BBa_K1680026
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1680026/annotation2472305/range2472305/1

https://synbiohub.org/public/igem/BBa_K1680026/annotation2472305/range2472305/1
type: Range
persistent identity: range2472305
display id: range2472305
version: 1
direction: direction/forward
top level: BBa_K1680026
start: 2290
end: 2878
orientation: inline

thrombin cleavage site
type: SequenceAnnotation
persistent identity: annotation2472237
display id: annotation2472237
version: 1
top level: BBa_K1680026
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1680026/annotation2472237/range2472237/1

https://synbiohub.org/public/igem/BBa_K1680026/annotation2472237/range2472237/1
type: Range
persistent identity: range2472237
display id: range2472237
version: 1
direction: direction/forward
top level: BBa_K1680026
start: 104
end: 121
orientation: inline

Lac operator
type: SequenceAnnotation
persistent identity: annotation2472194
display id: annotation2472194
version: 1
top level: BBa_K1680026
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1680026/annotation2472194/range2472194/1

https://synbiohub.org/public/igem/BBa_K1680026/annotation2472194/range2472194/1
type: Range
persistent identity: range2472194
display id: range2472194
version: 1
direction: direction/forward
top level: BBa_K1680026
start: 20
end: 44
orientation: inline

T7 terminator
type: SequenceAnnotation
persistent identity: annotation2472260
display id: annotation2472260
version: 1
top level: BBa_K1680026
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1680026/annotation2472260/range2472260/1

https://synbiohub.org/public/igem/BBa_K1680026/annotation2472260/range2472260/1
type: Range
persistent identity: range2472260
display id: range2472260
version: 1
direction: direction/forward
top level: BBa_K1680026
start: 683
end: 730
orientation: inline

tet promoter
type: SequenceAnnotation
persistent identity: annotation2472238
display id: annotation2472238
version: 1
top level: BBa_K1680026
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680026/annotation2472238/range2472238/1

https://synbiohub.org/public/igem/BBa_K1680026/annotation2472238/range2472238/1
type: Range
persistent identity: range2472238
display id: range2472238
version: 1
direction: direction/reverse
top level: BBa_K1680026
start: 212
end: 240
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation2472328
display id: annotation2472328
version: 1
top level: BBa_K1680026
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1680026/annotation2472328/range2472328/1

https://synbiohub.org/public/igem/BBa_K1680026/annotation2472328/range2472328/1
type: Range
persistent identity: range2472328
display id: range2472328
version: 1
direction: direction/forward
top level: BBa_K1680026
start: 3277
end: 3296
orientation: inline

f1 ori
type: SequenceAnnotation
persistent identity: annotation2472261
display id: annotation2472261
version: 1
top level: BBa_K1680026
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1680026/annotation2472261/range2472261/1

https://synbiohub.org/public/igem/BBa_K1680026/annotation2472261/range2472261/1
type: Range
persistent identity: range2472261
display id: range2472261
version: 1
direction: direction/forward
top level: BBa_K1680026
start: 767
end: 1222
orientation: inline

RBS.1 strong
type: SequenceAnnotation
persistent identity: annotation2472195
display id: annotation2472195
version: 1
top level: BBa_K1680026
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1680026/annotation2472195/range2472195/1

https://synbiohub.org/public/igem/BBa_K1680026/annotation2472195/range2472195/1
type: Range
persistent identity: range2472195
display id: range2472195
version: 1
direction: direction/forward
top level: BBa_K1680026
start: 53
end: 67
orientation: inline

AmpR
type: SequenceAnnotation
persistent identity: annotation2472289
display id: annotation2472289
version: 1
top level: BBa_K1680026
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1680026/annotation2472289/range2472289/1

https://synbiohub.org/public/igem/BBa_K1680026/annotation2472289/range2472289/1
type: Range
persistent identity: range2472289
display id: range2472289
version: 1
direction: direction/forward
top level: BBa_K1680026
start: 1337
end: 2197
orientation: inline

rrnB T1 terminator
type: SequenceAnnotation
persistent identity: annotation2472239
display id: annotation2472239
version: 1
top level: BBa_K1680026
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1680026/annotation2472239/range2472239/1

https://synbiohub.org/public/igem/BBa_K1680026/annotation2472239/range2472239/1
type: Range
persistent identity: range2472239
display id: range2472239
version: 1
direction: direction/forward
top level: BBa_K1680026
start: 410
end: 496
orientation: inline

BBa_K559010
type: ComponentDefinition
persistent identity: BBa K559010
display id: BBa_K559010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Halorhodopsin complete system
creator: Jacky, Fong Chuen, Loo
created: 2011-09-28T11:00:00Z
modified: 2015-05-08T01:12:41Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _727_
m user id: 0
owner id: 6123
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Halorhodopsin gene allow chloride ion to be pumped in by the level of light intensity. Halorhodopsin gene is under regulation by constitute T7 promoter to allow protein coding to be maximized, and the bidirectional terminator is added for high efficiency termination of both forward and backward transcription
mutable notes: The double terminator wass tested to stop effectively on the transcription of both forward and backward transcription to make our protein generator be a controlled system
mutable provenance: Halorhodopsin comes from part BBa_K559000, which is come from genomic sequence of N.pharaonis (genebank ID:J05199.1)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K559001
component: BBa_B0014
sequence annotation: BBa_B0014
sequence annotation: BBa_K559001
sequence: https://synbiohub.org/public/igem/BBa_K559010_sequence/1

BBa_K559001
type: Component
persistent identity: component2177574
display id: component2177574
version: 1
top level: BBa_K559010
access: public
definition: BBa_K559001

BBa_B0014
type: Component
persistent identity: component2177581
display id: component2177581
version: 1
top level: BBa_K559010
access: public
definition: https://synbiohub.org/public/igem/BBa_B0014/1

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2177581
display id: annotation2177581
version: 1
top level: BBa_K559010
location: https://synbiohub.org/public/igem/BBa_K559010/annotation2177581/range2177581/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_K559010/annotation2177581/range2177581/1
type: Range
persistent identity: range2177581
display id: range2177581
version: 1
direction: direction/reverse
top level: BBa_K559010
start: 737
end: 831
orientation: inline

BBa_K559001
type: SequenceAnnotation
persistent identity: annotation2177574
display id: annotation2177574
version: 1
top level: BBa_K559010
location: https://synbiohub.org/public/igem/BBa_K559010/annotation2177574/range2177574/1
component: BBa_K559001

https://synbiohub.org/public/igem/BBa_K559010/annotation2177574/range2177574/1
type: Range
persistent identity: range2177574
display id: range2177574
version: 1
direction: direction/reverse
top level: BBa_K559010
start: 1
end: 728
orientation: inline

T7start
type: ComponentDefinition
persistent identity: BBa J18912
display id: BBa_J18912
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 promoter + RBS + START
creator: Raik Gruenberg
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Strong T7 promoter and RBS including start codon for protein expression in E. coli or in-vitro. Part is provided in RFC 25 assembly format.
mutable notes: * sequence is taken from the commonly used pET3a expression vector * deleted XbaI site between prom. and RBS * T7 promoter sequence (1-19) equals the one in BBa_I712074 * Conservative choice of part boundaries and modifications -- the part's size could probably be reduced.
mutable provenance: pET3a expression vector
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_J18912_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2065586
display id: annotation2065586
version: 1
top level: T7start
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J18912/annotation2065586/range2065586/1

https://synbiohub.org/public/igem/BBa_J18912/annotation2065586/range2065586/1
type: Range
persistent identity: range2065586
display id: range2065586
version: 1
direction: direction/forward
top level: T7start
start: 81
end: 83
orientation: inline

BBa_K533001
type: ComponentDefinition
persistent identity: BBa K533001
display id: BBa_K533001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: OmpA-SH3
creator: ZHANG Yunxiao
created: 2011-09-28T11:00:00Z
modified: 2015-05-08T01:12:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _699_
m user id: 0
owner id: 6884
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part contains the N-terminal 180 amino acids of OmpA fused to SH3 domain in human Grb2 protein. The N-terminal of OmpA allows the moiety fused to it to be presented on to the outer membrane of E. Coli. SH3 domain allows for binding to multi-proline peptide in human cells, which functions as a crucial adaptor module in signaling. In our part, this is specially for binding to the proteins for transportation by E. Coli. All the coding sequence is under T7 promoter and lac repressor binding site. The parts is most efficiently presented onto the membrane when induced at 0.1mM IPTG at 18 centigrade in BL21 E. Coli strain.
mutable notes: High expression efficiency as well as inducible expression is most desirable when considering a coding sequence.
mutable provenance: OmpA is derived from the genomic DNA of BL21 (DE3) E. Coli strain while SH3 is from human Grb2.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: OmpA
sequence annotation: T7 promoter
sequence annotation: SH3
sequence annotation: lac operator
sequence: https://synbiohub.org/public/igem/BBa_K533001_sequence/1

OmpA
type: SequenceAnnotation
persistent identity: annotation2150408
display id: annotation2150408
version: 1
top level: BBa_K533001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K533001/annotation2150408/range2150408/1

https://synbiohub.org/public/igem/BBa_K533001/annotation2150408/range2150408/1
type: Range
persistent identity: range2150408
display id: range2150408
version: 1
direction: direction/forward
top level: BBa_K533001
start: 87
end: 632
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2150407
display id: annotation2150407
version: 1
top level: BBa_K533001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K533001/annotation2150407/range2150407/1

https://synbiohub.org/public/igem/BBa_K533001/annotation2150407/range2150407/1
type: Range
persistent identity: range2150407
display id: range2150407
version: 1
direction: direction/forward
top level: BBa_K533001
start: 1
end: 18
orientation: inline

SH3
type: SequenceAnnotation
persistent identity: annotation2150409
display id: annotation2150409
version: 1
top level: BBa_K533001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K533001/annotation2150409/range2150409/1

https://synbiohub.org/public/igem/BBa_K533001/annotation2150409/range2150409/1
type: Range
persistent identity: range2150409
display id: range2150409
version: 1
direction: direction/forward
top level: BBa_K533001
start: 633
end: 815
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation2150406
display id: annotation2150406
version: 1
top level: BBa_K533001
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K533001/annotation2150406/range2150406/1

https://synbiohub.org/public/igem/BBa_K533001/annotation2150406/range2150406/1
type: Range
persistent identity: range2150406
display id: range2150406
version: 1
direction: direction/forward
top level: BBa_K533001
start: 20
end: 44
orientation: inline

T7stop
type: ComponentDefinition
persistent identity: BBa J18913
display id: BBa_J18913
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 terminator (incl. STOP)
creator: Raik Gruenberg
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: T7 terminator for protein expression in E. coli or in vitro. Part is provided in R.F.C. 25 assembly format.
mutable notes: * taken from pET3a expression plasmid * added two STOP to 5' * conservative choice of part boundary, could probably be shortened
mutable provenance: pET3a expression plasmid
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_J18913_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2065587
display id: annotation2065587
version: 1
top level: T7stop
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_J18913/annotation2065587/range2065587/1

https://synbiohub.org/public/igem/BBa_J18913/annotation2065587/range2065587/1
type: Range
persistent identity: range2065587
display id: range2065587
version: 1
direction: direction/forward
top level: T7stop
start: 1
end: 6
orientation: inline

BBa_K559001
type: ComponentDefinition
persistent identity: BBa K559001
display id: BBa_K559001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Halorhodopsin under T7 promoter
creator: Jacky, Fong Chuen, Loo
created: 2011-09-28T11:00:00Z
modified: 2015-05-08T01:12:41Z
discontinued: false
dominant: false
experience: experience/None
group u list: _727_
m user id: 0
owner id: 6123
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Halorhodopsin under constitute T7 promoter to regulate the gene transcription to be maximized and no restrict to other regulatory factor
mutable notes: The coding sequence of N.pharaonis J05199.1 is selected from 61nt to 700nt and the T7 promotor placed in front by 3A assembly
mutable provenance: Halorhodopsin come from N.pharaonis, gene bank J05199.1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: lac repressor
sequence annotation: Halorhodopsin
sequence annotation: RBS
sequence annotation: T7 promotor
sequence: https://synbiohub.org/public/igem/BBa_K559001_sequence/1

lac repressor
type: SequenceAnnotation
persistent identity: annotation2177553
display id: annotation2177553
version: 1
top level: BBa_K559001
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K559001/annotation2177553/range2177553/1

https://synbiohub.org/public/igem/BBa_K559001/annotation2177553/range2177553/1
type: Range
persistent identity: range2177553
display id: range2177553
version: 1
direction: direction/forward
top level: BBa_K559001
start: 22
end: 42
orientation: inline

Halorhodopsin
type: SequenceAnnotation
persistent identity: annotation2177554
display id: annotation2177554
version: 1
top level: BBa_K559001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K559001/annotation2177554/range2177554/1

https://synbiohub.org/public/igem/BBa_K559001/annotation2177554/range2177554/1
type: Range
persistent identity: range2177554
display id: range2177554
version: 1
direction: direction/forward
top level: BBa_K559001
start: 89
end: 728
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2177555
display id: annotation2177555
version: 1
top level: BBa_K559001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K559001/annotation2177555/range2177555/1

https://synbiohub.org/public/igem/BBa_K559001/annotation2177555/range2177555/1
type: Range
persistent identity: range2177555
display id: range2177555
version: 1
direction: direction/forward
top level: BBa_K559001
start: 66
end: 77
orientation: inline

T7 promotor
type: SequenceAnnotation
persistent identity: annotation2177551
display id: annotation2177551
version: 1
top level: BBa_K559001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K559001/annotation2177551/range2177551/1

https://synbiohub.org/public/igem/BBa_K559001/annotation2177551/range2177551/1
type: Range
persistent identity: range2177551
display id: range2177551
version: 1
direction: direction/forward
top level: BBa_K559001
start: 1
end: 16
orientation: inline

BBa_K921002
type: ComponentDefinition
persistent identity: BBa K921002
display id: BBa_K921002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 RNAP + IPTG->PoPs (Mutant III)
creator: Eric Pederson
created: 2012-09-30T11:00:00Z
modified: 2015-05-08T01:13:46Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1186_
m user id: 0
owner id: 12713
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is a mutant T7 promoter that includes a lacO sequence. This is repressible by the LacI protein and is induced in the presence of IPTG. The promoter sits in the 5' region of the gene of interest and initiates transcription when the cell expresses T7 RNA polymerase. Be sure you are using a cell strain that includes this gene (DE3).
mutable notes: There are three portions to the T7 promoter: the recognition site, the melting box (or TATA box) and the initation site. Mutations in these sequences caused differential behavior from the wild type.
mutable provenance: The part consists of a sequences that resemble a T7 promoter and the lacO sequence found in E. coli.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/T7
role: SO:0001207
sequence annotation: Initiation
sequence annotation: T7 Promoter
sequence annotation: LacO
sequence: https://synbiohub.org/public/igem/BBa_K921002_sequence/1

Initiation
type: SequenceAnnotation
persistent identity: annotation2205318
display id: annotation2205318
version: 1
top level: BBa_K921002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K921002/annotation2205318/range2205318/1

https://synbiohub.org/public/igem/BBa_K921002/annotation2205318/range2205318/1
type: Range
persistent identity: range2205318
display id: range2205318
version: 1
direction: direction/forward
top level: BBa_K921002
start: 18
end: 20
orientation: inline

T7 Promoter
type: SequenceAnnotation
persistent identity: annotation2205316
display id: annotation2205316
version: 1
top level: BBa_K921002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K921002/annotation2205316/range2205316/1

https://synbiohub.org/public/igem/BBa_K921002/annotation2205316/range2205316/1
type: Range
persistent identity: range2205316
display id: range2205316
version: 1
direction: direction/forward
top level: BBa_K921002
start: 1
end: 20
orientation: inline

LacO
type: SequenceAnnotation
persistent identity: annotation2205317
display id: annotation2205317
version: 1
top level: BBa_K921002
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K921002/annotation2205317/range2205317/1

https://synbiohub.org/public/igem/BBa_K921002/annotation2205317/range2205317/1
type: Range
persistent identity: range2205317
display id: range2205317
version: 1
direction: direction/forward
top level: BBa_K921002
start: 19
end: 47
orientation: inline

BBa_K921000
type: ComponentDefinition
persistent identity: BBa K921000
display id: BBa_K921000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 RNAP + IPTG->PoPs (Mutant I)
creator: Eric Pederson
created: 2012-09-30T11:00:00Z
modified: 2015-05-08T01:13:46Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1186_
m user id: 0
owner id: 12713
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is a mutant T7 promoter that includes a lacO sequence. This is repressible by the LacI protein and is induced in the presence of IPTG. The promoter sits in the 5' region of the gene of interest and initiates transcription when the cell expresses T7 RNA polymerase. Be sure you are using a cell strain that includes this gene (DE3).
mutable notes: There are three portions to the T7 promoter: the recognition site, the melting box (or TATA box) and the initation site. Mutations in these sequences caused differential behavior from the wild type.
mutable provenance: The part consists of a sequences that resemble a T7 promoter and the lacO sequence found in E. coli.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/T7
role: SO:0001207
sequence annotation: Initiation
sequence annotation: T7 promoter
sequence annotation: LacO
sequence: https://synbiohub.org/public/igem/BBa_K921000_sequence/1

Initiation
type: SequenceAnnotation
persistent identity: annotation2205291
display id: annotation2205291
version: 1
top level: BBa_K921000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K921000/annotation2205291/range2205291/1

https://synbiohub.org/public/igem/BBa_K921000/annotation2205291/range2205291/1
type: Range
persistent identity: range2205291
display id: range2205291
version: 1
direction: direction/forward
top level: BBa_K921000
start: 19
end: 20
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2205289
display id: annotation2205289
version: 1
top level: BBa_K921000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K921000/annotation2205289/range2205289/1

https://synbiohub.org/public/igem/BBa_K921000/annotation2205289/range2205289/1
type: Range
persistent identity: range2205289
display id: range2205289
version: 1
direction: direction/forward
top level: BBa_K921000
start: 1
end: 19
orientation: inline

LacO
type: SequenceAnnotation
persistent identity: annotation2205290
display id: annotation2205290
version: 1
top level: BBa_K921000
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K921000/annotation2205290/range2205290/1

https://synbiohub.org/public/igem/BBa_K921000/annotation2205290/range2205290/1
type: Range
persistent identity: range2205290
display id: range2205290
version: 1
direction: direction/forward
top level: BBa_K921000
start: 19
end: 46
orientation: inline

T7p
type: ComponentDefinition
persistent identity: BBa K133041
display id: BBa_K133041
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 promoter expression plasmid backbone
creator: Ana Lasic
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:09:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _231_
m user id: 0
owner id: 3352
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
sequence annotation: amp promoter
sequence annotation: partial BioBrick prefix (missing EcoRI)
sequence annotation: pBR322
sequence annotation: BioBrick suffix
sequence annotation: kanamycin2 marker
sequence annotation: lacO regulatory site
sequence annotation: T7 promoter
sequence annotation: beta-lactamase (ampicillin resistance marker)
sequence: https://synbiohub.org/public/igem/BBa_K133041_sequence/1

amp promoter
type: SequenceAnnotation
persistent identity: annotation1999657
display id: annotation1999657
version: 1
top level: T7p
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K133041/annotation1999657/range1999657/1

https://synbiohub.org/public/igem/BBa_K133041/annotation1999657/range1999657/1
type: Range
persistent identity: range1999657
display id: range1999657
version: 1
direction: direction/reverse
top level: T7p
start: 2969
end: 2997
orientation: inline

partial BioBrick prefix (missing EcoRI)
type: SequenceAnnotation
persistent identity: annotation1999660
display id: annotation1999660
version: 1
top level: T7p
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K133041/annotation1999660/range1999660/1

https://synbiohub.org/public/igem/BBa_K133041/annotation1999660/range1999660/1
type: Range
persistent identity: range1999660
display id: range1999660
version: 1
direction: direction/forward
top level: T7p
start: 3258
end: 3273
orientation: inline

pBR322
type: SequenceAnnotation
persistent identity: annotation1999653
display id: annotation1999653
version: 1
top level: T7p
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K133041/annotation1999653/range1999653/1

https://synbiohub.org/public/igem/BBa_K133041/annotation1999653/range1999653/1
type: Range
persistent identity: range1999653
display id: range1999653
version: 1
direction: direction/reverse
top level: T7p
start: 261
end: 880
orientation: inline

BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation1999654
display id: annotation1999654
version: 1
top level: T7p
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K133041/annotation1999654/range1999654/1

https://synbiohub.org/public/igem/BBa_K133041/annotation1999654/range1999654/1
type: Range
persistent identity: range1999654
display id: range1999654
version: 1
direction: direction/forward
top level: T7p
start: 1
end: 21
orientation: inline

kanamycin2 marker
type: SequenceAnnotation
persistent identity: annotation1999655
display id: annotation1999655
version: 1
top level: T7p
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K133041/annotation1999655/range1999655/1

https://synbiohub.org/public/igem/BBa_K133041/annotation1999655/range1999655/1
type: Range
persistent identity: range1999655
display id: range1999655
version: 1
direction: direction/reverse
top level: T7p
start: 1097
end: 1912
orientation: inline

lacO regulatory site
type: SequenceAnnotation
persistent identity: annotation1999659
display id: annotation1999659
version: 1
top level: T7p
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K133041/annotation1999659/range1999659/1

https://synbiohub.org/public/igem/BBa_K133041/annotation1999659/range1999659/1
type: Range
persistent identity: range1999659
display id: range1999659
version: 1
direction: direction/forward
top level: T7p
start: 3191
end: 3218
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation1999658
display id: annotation1999658
version: 1
top level: T7p
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K133041/annotation1999658/range1999658/1

https://synbiohub.org/public/igem/BBa_K133041/annotation1999658/range1999658/1
type: Range
persistent identity: range1999658
display id: range1999658
version: 1
direction: direction/forward
top level: T7p
start: 3173
end: 3191
orientation: inline

beta-lactamase (ampicillin resistance marker)
type: SequenceAnnotation
persistent identity: annotation1999656
display id: annotation1999656
version: 1
top level: T7p
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K133041/annotation1999656/range1999656/1

https://synbiohub.org/public/igem/BBa_K133041/annotation1999656/range1999656/1
type: Range
persistent identity: range1999656
display id: range1999656
version: 1
direction: direction/reverse
top level: T7p
start: 2067
end: 2927
orientation: inline

BBa_K1428000
type: ComponentDefinition
persistent identity: BBa K1428000
display id: BBa_K1428000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 promoter-driven BMC structural genes with a His6-tagged component for purification
creator: Lai Hei Ming
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:23Z
discontinued: false
dominant: false
group u list: _1806_
m user id: 0
owner id: 20650
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: T7 promoter diving the expression of His6-tagged EutS, with subsequent Plac-driven EutM, EutN, EutL, EutK
mutable notes: Clone BBa_K311004 into pET28a via NheI and NdeI
mutable provenance: BBa_K311004, pET28a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: signal peptide
sequence annotation: fusion linker (TGS)
sequence annotation: T7 RBS
sequence: https://synbiohub.org/public/igem/BBa_K1428000_sequence/1

signal peptide
type: SequenceAnnotation
persistent identity: annotation2417041
display id: annotation2417041
version: 1
top level: BBa_K1428000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1428000/annotation2417041/range2417041/1

https://synbiohub.org/public/igem/BBa_K1428000/annotation2417041/range2417041/1
type: Range
persistent identity: range2417041
display id: range2417041
version: 1
direction: direction/forward
top level: BBa_K1428000
start: 37
end: 93
orientation: inline

fusion linker (TGS)
type: SequenceAnnotation
persistent identity: annotation2417042
display id: annotation2417042
version: 1
top level: BBa_K1428000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1428000/annotation2417042/range2417042/1

https://synbiohub.org/public/igem/BBa_K1428000/annotation2417042/range2417042/1
type: Range
persistent identity: range2417042
display id: range2417042
version: 1
direction: direction/forward
top level: BBa_K1428000
start: 94
end: 103
orientation: inline

T7 RBS
type: SequenceAnnotation
persistent identity: annotation2417040
display id: annotation2417040
version: 1
top level: BBa_K1428000
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1428000/annotation2417040/range2417040/1

https://synbiohub.org/public/igem/BBa_K1428000/annotation2417040/range2417040/1
type: Range
persistent identity: range2417040
display id: range2417040
version: 1
direction: direction/forward
top level: BBa_K1428000
start: 13
end: 29
orientation: inline

BBa_K533002
type: ComponentDefinition
persistent identity: BBa K533002
display id: BBa_K533002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: OmpA-HIV protease site-SH3
creator: ZHANG Yunxiao
created: 2011-09-28T11:00:00Z
modified: 2015-05-08T01:12:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _699_
m user id: 0
owner id: 6884
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Added HIV protease site between OmpA and SH3 for easier release.
mutable notes: The HIV protease site is embedded in the protein so that a proteolytic cleavage can release the binding cassette.
mutable provenance: OmpA is from BL21(DE3) E. Coli genomic DNA. SH3 is from human Grb2 protein. HIV protease site is from a former part.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: OmpA
sequence annotation: BBa_I712015
sequence annotation: T7 promoter
sequence annotation: SH3
sequence annotation: lacI
sequence: https://synbiohub.org/public/igem/BBa_K533002_sequence/1

OmpA
type: SequenceAnnotation
persistent identity: annotation2150424
display id: annotation2150424
version: 1
top level: BBa_K533002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K533002/annotation2150424/range2150424/1

https://synbiohub.org/public/igem/BBa_K533002/annotation2150424/range2150424/1
type: Range
persistent identity: range2150424
display id: range2150424
version: 1
direction: direction/forward
top level: BBa_K533002
start: 87
end: 633
orientation: inline

BBa_I712015
type: SequenceAnnotation
persistent identity: annotation2146914
display id: annotation2146914
version: 1
top level: BBa_K533002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K533002/annotation2146914/range2146914/1

https://synbiohub.org/public/igem/BBa_K533002/annotation2146914/range2146914/1
type: Range
persistent identity: range2146914
display id: range2146914
version: 1
direction: direction/forward
top level: BBa_K533002
start: 634
end: 675
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2146913
display id: annotation2146913
version: 1
top level: BBa_K533002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K533002/annotation2146913/range2146913/1

https://synbiohub.org/public/igem/BBa_K533002/annotation2146913/range2146913/1
type: Range
persistent identity: range2146913
display id: range2146913
version: 1
direction: direction/forward
top level: BBa_K533002
start: 1
end: 18
orientation: inline

SH3
type: SequenceAnnotation
persistent identity: annotation2150425
display id: annotation2150425
version: 1
top level: BBa_K533002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K533002/annotation2150425/range2150425/1

https://synbiohub.org/public/igem/BBa_K533002/annotation2150425/range2150425/1
type: Range
persistent identity: range2150425
display id: range2150425
version: 1
direction: direction/forward
top level: BBa_K533002
start: 676
end: 857
orientation: inline

lacI
type: SequenceAnnotation
persistent identity: annotation2150423
display id: annotation2150423
version: 1
top level: BBa_K533002
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K533002/annotation2150423/range2150423/1

https://synbiohub.org/public/igem/BBa_K533002/annotation2150423/range2150423/1
type: Range
persistent identity: range2150423
display id: range2150423
version: 1
direction: direction/forward
top level: BBa_K533002
start: 20
end: 44
orientation: inline

T7RSR
type: ComponentDefinition
persistent identity: BBa K786031
display id: BBa_K786031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7promoter-RSR-T7teminator
creator: Kaiyin Feng
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:22Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1041_
m user id: 0
owner id: 14280
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: It is a repeat-spacer-repeat region designed according to E.coli's CRISPR/Cas system. It also includes a T7 promoter and T7 terminator. THe spacer is designed to target on a segment of non-coding-sequence in E.coli's genome. This biobrick can be transformed into E.coli cells to hopefully construct a suicide system with E.coli's CRISPR/Cas system.
mutable notes: The repeat-spacer-repeat region design should be consistent with the operating principles of E.coli's CRISPR/cas system.
mutable provenance: The 'repeat' sequence in CRISPR/cas system of E.coli strain K12.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: T7-promoter
sequence annotation: T7-terminator
sequence annotation: repeat-spacer-repeat region
sequence: https://synbiohub.org/public/igem/BBa_K786031_sequence/1

T7-promoter
type: SequenceAnnotation
persistent identity: annotation2203441
display id: annotation2203441
version: 1
top level: T7RSR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K786031/annotation2203441/range2203441/1

https://synbiohub.org/public/igem/BBa_K786031/annotation2203441/range2203441/1
type: Range
persistent identity: range2203441
display id: range2203441
version: 1
direction: direction/forward
top level: T7RSR
start: 1
end: 23
orientation: inline

T7-terminator
type: SequenceAnnotation
persistent identity: annotation2203443
display id: annotation2203443
version: 1
top level: T7RSR
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K786031/annotation2203443/range2203443/1

https://synbiohub.org/public/igem/BBa_K786031/annotation2203443/range2203443/1
type: Range
persistent identity: range2203443
display id: range2203443
version: 1
direction: direction/forward
top level: T7RSR
start: 812
end: 956
orientation: inline

repeat-spacer-repeat region
type: SequenceAnnotation
persistent identity: annotation2203442
display id: annotation2203442
version: 1
top level: T7RSR
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K786031/annotation2203442/range2203442/1

https://synbiohub.org/public/igem/BBa_K786031/annotation2203442/range2203442/1
type: Range
persistent identity: range2203442
display id: range2203442
version: 1
direction: direction/forward
top level: T7RSR
start: 31
end: 803
orientation: inline

BBa_K1614000
type: ComponentDefinition
persistent identity: BBa K1614000
display id: BBa_K1614000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 promoter for expression of functional RNA
creator: Stefan Holderbach
created: 2015-09-13T11:00:00Z
modified: 2015-09-14T12:29:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2031_
m user id: 12380
owner id: 16422
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Test
mutable notes: None.
mutable provenance: T7 phage minimal promoter (consensus)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: T7 promoter
sequence: https://synbiohub.org/public/igem/BBa_K1614000_sequence/1

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2454728
display id: annotation2454728
version: 1
top level: BBa_K1614000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1614000/annotation2454728/range2454728/1

https://synbiohub.org/public/igem/BBa_K1614000/annotation2454728/range2454728/1
type: Range
persistent identity: range2454728
display id: range2454728
version: 1
direction: direction/forward
top level: BBa_K1614000
start: 1
end: 17
orientation: inline

BBa_K533003
type: ComponentDefinition
persistent identity: BBa K533003
display id: BBa_K533003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: OmpA-HIV aspartyl protease
creator: ZHANG Yunxiao
created: 2011-09-28T11:00:00Z
modified: 2015-05-08T01:12:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _699_
m user id: 0
owner id: 6884
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: OmpA is used to present HIV aspartyl protease outside the E. Coli membrane so that it can cut other proteins with the specific sequence outside in the media.
mutable notes: Since HIV protease is a dimer, dimerization is a great concern to its function. Function of this protease is tested in BL21.
mutable provenance: HIV protease sequence is from former part BBa_I712667. OmpA sequence is from BL21(DE3) e. Coli strain.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: START
sequence annotation: rbs
sequence annotation: HIV protease
sequence annotation: OmpA
sequence annotation: STOP
sequence annotation: lacI
sequence annotation: T7
sequence annotation: BBa_I712667
sequence: https://synbiohub.org/public/igem/BBa_K533003_sequence/1

START
type: SequenceAnnotation
persistent identity: annotation2146916
display id: annotation2146916
version: 1
top level: BBa_K533003
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K533003/annotation2146916/range2146916/1

https://synbiohub.org/public/igem/BBa_K533003/annotation2146916/range2146916/1
type: Range
persistent identity: range2146916
display id: range2146916
version: 1
direction: direction/forward
top level: BBa_K533003
start: 87
end: 89
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2150442
display id: annotation2150442
version: 1
top level: BBa_K533003
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K533003/annotation2150442/range2150442/1

https://synbiohub.org/public/igem/BBa_K533003/annotation2150442/range2150442/1
type: Range
persistent identity: range2150442
display id: range2150442
version: 1
direction: direction/forward
top level: BBa_K533003
start: 75
end: 79
orientation: inline

HIV protease
type: SequenceAnnotation
persistent identity: annotation2146915
display id: annotation2146915
version: 1
top level: BBa_K533003
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K533003/annotation2146915/range2146915/1

https://synbiohub.org/public/igem/BBa_K533003/annotation2146915/range2146915/1
type: Range
persistent identity: range2146915
display id: range2146915
version: 1
direction: direction/forward
top level: BBa_K533003
start: 633
end: 935
orientation: inline

OmpA
type: SequenceAnnotation
persistent identity: annotation2150441
display id: annotation2150441
version: 1
top level: BBa_K533003
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K533003/annotation2150441/range2150441/1

https://synbiohub.org/public/igem/BBa_K533003/annotation2150441/range2150441/1
type: Range
persistent identity: range2150441
display id: range2150441
version: 1
direction: direction/forward
top level: BBa_K533003
start: 87
end: 632
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation2146917
display id: annotation2146917
version: 1
top level: BBa_K533003
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K533003/annotation2146917/range2146917/1

https://synbiohub.org/public/igem/BBa_K533003/annotation2146917/range2146917/1
type: Range
persistent identity: range2146917
display id: range2146917
version: 1
direction: direction/forward
top level: BBa_K533003
start: 933
end: 935
orientation: inline

lacI
type: SequenceAnnotation
persistent identity: annotation2150440
display id: annotation2150440
version: 1
top level: BBa_K533003
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K533003/annotation2150440/range2150440/1

https://synbiohub.org/public/igem/BBa_K533003/annotation2150440/range2150440/1
type: Range
persistent identity: range2150440
display id: range2150440
version: 1
direction: direction/forward
top level: BBa_K533003
start: 20
end: 44
orientation: inline

T7
type: SequenceAnnotation
persistent identity: annotation2150439
display id: annotation2150439
version: 1
top level: BBa_K533003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K533003/annotation2150439/range2150439/1

https://synbiohub.org/public/igem/BBa_K533003/annotation2150439/range2150439/1
type: Range
persistent identity: range2150439
display id: range2150439
version: 1
direction: direction/forward
top level: BBa_K533003
start: 1
end: 18
orientation: inline

BBa_I712667
type: SequenceAnnotation
persistent identity: annotation2146918
display id: annotation2146918
version: 1
top level: BBa_K533003
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K533003/annotation2146918/range2146918/1

https://synbiohub.org/public/igem/BBa_K533003/annotation2146918/range2146918/1
type: Range
persistent identity: range2146918
display id: range2146918
version: 1
direction: direction/reverse
top level: BBa_K533003
start: 633
end: 935
orientation: inline

BBa_K921001
type: ComponentDefinition
persistent identity: BBa K921001
display id: BBa_K921001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 RNAP + IPTG->PoPs (Mutant II)
creator: Eric Pederson
created: 2012-09-30T11:00:00Z
modified: 2015-05-08T01:13:46Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1186_
m user id: 0
owner id: 12713
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a mutant T7 promoter that includes a lacO sequence. This is repressible by the LacI protein and is induced in the presence of IPTG. The promoter sits in the 5' region of the gene of interest and initiates transcription when the cell expresses T7 RNA polymerase. Be sure you are using a cell strain that includes this gene (DE3).
mutable notes: There are three portions to the T7 promoter: the recognition site, the melting box (or TATA box) and the initation site. Mutations in these sequences caused differential behavior from the wild type.
mutable provenance: The part consists of a sequences that resemble a T7 promoter and the lacO sequence found in E. coli.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/T7
role: SO:0001207
sequence annotation: LacO
sequence annotation: T7 Promoter
sequence annotation: Initiation
sequence: https://synbiohub.org/public/igem/BBa_K921001_sequence/1

LacO
type: SequenceAnnotation
persistent identity: annotation2205297
display id: annotation2205297
version: 1
top level: BBa_K921001
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K921001/annotation2205297/range2205297/1

https://synbiohub.org/public/igem/BBa_K921001/annotation2205297/range2205297/1
type: Range
persistent identity: range2205297
display id: range2205297
version: 1
direction: direction/forward
top level: BBa_K921001
start: 22
end: 48
orientation: inline

T7 Promoter
type: SequenceAnnotation
persistent identity: annotation2205296
display id: annotation2205296
version: 1
top level: BBa_K921001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K921001/annotation2205296/range2205296/1

https://synbiohub.org/public/igem/BBa_K921001/annotation2205296/range2205296/1
type: Range
persistent identity: range2205296
display id: range2205296
version: 1
direction: direction/forward
top level: BBa_K921001
start: 1
end: 20
orientation: inline

Initiation
type: SequenceAnnotation
persistent identity: annotation2205298
display id: annotation2205298
version: 1
top level: BBa_K921001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K921001/annotation2205298/range2205298/1

https://synbiohub.org/public/igem/BBa_K921001/annotation2205298/range2205298/1
type: Range
persistent identity: range2205298
display id: range2205298
version: 1
direction: direction/forward
top level: BBa_K921001
start: 18
end: 20
orientation: inline